2810-4341

5-(5-ethyl-1,3-benzoxazol-2-yl)-2-methylaniline

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_325
Screen concentration 181.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 774970
SMILES CCC1=CC2=C(C=C1)OC(=N2)C3=CC(=C(C=C3)C)N
Standardized SMILES CCc1ccc2oc(nc2c1)c3ccc(C)c(N)c3
Molecular weight 252.311
ALogP 3.96
H-bond donor count 1
H-bond acceptor count 3
Response signature amide catabolism

Pool Growth Kinetics
% growth inhibition (Het. pool) 17.23
% growth inhibition (Hom. pool) 11.26


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 774970
Download HIP data (tab-delimited text)  (excel)
Gene:CAB2(YIL083C)|FD-Score:-3.34|P-value:4.17E-4|Clearance:0||SGD DESC:Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity) Gene:CCT6(YDR188W)|FD-Score:4.16|P-value:1.58E-5|Clearance:0.13||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CDC12(YHR107C)|FD-Score:-3.42|P-value:3.14E-4|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:COF1(YLL050C)|FD-Score:3.9|P-value:4.72E-5|Clearance:0.05||SGD DESC:Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes Gene:GCD2(YGR083C)|FD-Score:3.59|P-value:1.62E-4|Clearance:0.12||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:INO80(YGL150C)|FD-Score:3.73|P-value:9.47E-5|Clearance:0.12||SGD DESC:ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription Gene:MPE1(YKL059C)|FD-Score:4.32|P-value:7.65E-6|Clearance:0.16||SGD DESC:Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif Gene:PRP2(YNR011C)|FD-Score:3.61|P-value:1.52E-4|Clearance:0.02||SGD DESC:RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 Gene:RFC3(YNL290W)|FD-Score:5.15|P-value:1.34E-7|Clearance:0.82||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RFT1(YBL020W)|FD-Score:3.11|P-value:9.40E-4|Clearance:0.04||SGD DESC:Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein Gene:RPB10(YOR210W)|FD-Score:3.26|P-value:5.57E-4|Clearance:0.15||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:SMC1(YFL008W)|FD-Score:-4.01|P-value:3.07E-5|Clearance:0||SGD DESC:Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure Gene:SMD3(YLR147C)|FD-Score:-4.44|P-value:4.44E-6|Clearance:0||SGD DESC:Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 Gene:SPP381(YBR152W)|FD-Score:3.85|P-value:5.92E-5|Clearance:0.12||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:UTP5(YDR398W)|FD-Score:4.03|P-value:2.78E-5|Clearance:0.13||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YDR187C(YDR187C_d)|FD-Score:6.06|P-value:6.61E-10|Clearance:0.92||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YJR012C(YJR012C_p)|FD-Score:3.48|P-value:2.54E-4|Clearance:0.22||SGD DESC:Essential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W Gene:YOR203W(YOR203W_d)|FD-Score:-4.1|P-value:2.05E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation Gene:CAB2(YIL083C)|FD-Score:-3.34|P-value:4.17E-4|Clearance:0||SGD DESC:Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity) Gene:CCT6(YDR188W)|FD-Score:4.16|P-value:1.58E-5|Clearance:0.13||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CDC12(YHR107C)|FD-Score:-3.42|P-value:3.14E-4|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:COF1(YLL050C)|FD-Score:3.9|P-value:4.72E-5|Clearance:0.05||SGD DESC:Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes Gene:GCD2(YGR083C)|FD-Score:3.59|P-value:1.62E-4|Clearance:0.12||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:INO80(YGL150C)|FD-Score:3.73|P-value:9.47E-5|Clearance:0.12||SGD DESC:ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription Gene:MPE1(YKL059C)|FD-Score:4.32|P-value:7.65E-6|Clearance:0.16||SGD DESC:Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif Gene:PRP2(YNR011C)|FD-Score:3.61|P-value:1.52E-4|Clearance:0.02||SGD DESC:RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 Gene:RFC3(YNL290W)|FD-Score:5.15|P-value:1.34E-7|Clearance:0.82||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RFT1(YBL020W)|FD-Score:3.11|P-value:9.40E-4|Clearance:0.04||SGD DESC:Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein Gene:RPB10(YOR210W)|FD-Score:3.26|P-value:5.57E-4|Clearance:0.15||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:SMC1(YFL008W)|FD-Score:-4.01|P-value:3.07E-5|Clearance:0||SGD DESC:Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure Gene:SMD3(YLR147C)|FD-Score:-4.44|P-value:4.44E-6|Clearance:0||SGD DESC:Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 Gene:SPP381(YBR152W)|FD-Score:3.85|P-value:5.92E-5|Clearance:0.12||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:UTP5(YDR398W)|FD-Score:4.03|P-value:2.78E-5|Clearance:0.13||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YDR187C(YDR187C_d)|FD-Score:6.06|P-value:6.61E-10|Clearance:0.92||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YJR012C(YJR012C_p)|FD-Score:3.48|P-value:2.54E-4|Clearance:0.22||SGD DESC:Essential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W Gene:YOR203W(YOR203W_d)|FD-Score:-4.1|P-value:2.05E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 774970
Download HOP data (tab-delimited text)  (excel)
Gene:ALK2(YBL009W)|FD-Score:-3.18|P-value:7.42E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins Gene:COX9(YDL067C)|FD-Score:3.27|P-value:5.41E-4||SGD DESC:Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CRT10(YOL063C)|FD-Score:-3.32|P-value:4.44E-4||SGD DESC:Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat Gene:CRZ1(YNL027W)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress Gene:DAL81(YIR023W)|FD-Score:12.2|P-value:1.98E-34||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DSF2(YBR007C_p)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:FUN30(YAL019W)|FD-Score:4.59|P-value:2.21E-6||SGD DESC:Snf2p family member with ATP-dependent chromatin remodeling activity; has a role in silencing at the mating type locus, telomeres and centromeres; enriched at centromeres and is required for correct chromatin structure around centromeres, as well as at the boundary element of the silent HMR; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection of DSBs; potential Cdc28p substrate Gene:GUP1(YGL084C)|FD-Score:-4.16|P-value:1.62E-5||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HMO1(YDR174W)|FD-Score:4.82|P-value:7.19E-7||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:HOM6(YJR139C)|FD-Score:-3.75|P-value:8.96E-5||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:LSM6(YDR378C)|FD-Score:3.24|P-value:6.04E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MRE11(YMR224C)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:MSA1(YOR066W)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:OAF3(YKR064W)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:PRO2(YOR323C)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RCK1(YGL158W)|FD-Score:-4.08|P-value:2.29E-5||SGD DESC:Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication Gene:RDL2(YOR286W)|FD-Score:-4.17|P-value:1.49E-5||SGD DESC:Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss Gene:RKM4(YDR257C)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein Gene:RPL13B(YMR142C)|FD-Score:5|P-value:2.92E-7||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RPL20A(YMR242C)|FD-Score:3.95|P-value:3.89E-5||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPO41(YFL036W)|FD-Score:4.33|P-value:7.41E-6||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RPS18B(YML026C)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS24A(YER074W)|FD-Score:5.25|P-value:7.80E-8||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:SPO16(YHR153C)|FD-Score:4.35|P-value:6.75E-6||SGD DESC:Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation Gene:SSD1(YDR293C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:TRM10(YOL093W)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:tRNA methyltransferase; methylates the N-1 position of guanosine in tRNAs; protein abundance increases in response to DNA replication stress Gene:TRP1(YDR007W)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:YAL067W-A(YAL067W-A_p)|FD-Score:3.82|P-value:6.75E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDR056C(YDR056C_p)|FD-Score:3.16|P-value:7.93E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein Gene:YDR124W(YDR124W_p)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor Gene:YDR491C(YDR491C_d)|FD-Score:3.74|P-value:9.35E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL067C(YEL067C_p)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YER135C(YER135C_d)|FD-Score:-4|P-value:3.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YLR279W(YLR279W_d)|FD-Score:4.57|P-value:2.41E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR014C(YPR014C_d)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YSC84(YHR016C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication Gene:YVC1(YOR087W)|FD-Score:-3.37|P-value:3.71E-4||SGD DESC:Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock Gene:ALK2(YBL009W)|FD-Score:-3.18|P-value:7.42E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins Gene:COX9(YDL067C)|FD-Score:3.27|P-value:5.41E-4||SGD DESC:Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CRT10(YOL063C)|FD-Score:-3.32|P-value:4.44E-4||SGD DESC:Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat Gene:CRZ1(YNL027W)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress Gene:DAL81(YIR023W)|FD-Score:12.2|P-value:1.98E-34||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DSF2(YBR007C_p)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:FUN30(YAL019W)|FD-Score:4.59|P-value:2.21E-6||SGD DESC:Snf2p family member with ATP-dependent chromatin remodeling activity; has a role in silencing at the mating type locus, telomeres and centromeres; enriched at centromeres and is required for correct chromatin structure around centromeres, as well as at the boundary element of the silent HMR; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection of DSBs; potential Cdc28p substrate Gene:GUP1(YGL084C)|FD-Score:-4.16|P-value:1.62E-5||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HMO1(YDR174W)|FD-Score:4.82|P-value:7.19E-7||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:HOM6(YJR139C)|FD-Score:-3.75|P-value:8.96E-5||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:LSM6(YDR378C)|FD-Score:3.24|P-value:6.04E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MRE11(YMR224C)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:MSA1(YOR066W)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:OAF3(YKR064W)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:PRO2(YOR323C)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RCK1(YGL158W)|FD-Score:-4.08|P-value:2.29E-5||SGD DESC:Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication Gene:RDL2(YOR286W)|FD-Score:-4.17|P-value:1.49E-5||SGD DESC:Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss Gene:RKM4(YDR257C)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein Gene:RPL13B(YMR142C)|FD-Score:5|P-value:2.92E-7||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RPL20A(YMR242C)|FD-Score:3.95|P-value:3.89E-5||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPO41(YFL036W)|FD-Score:4.33|P-value:7.41E-6||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RPS18B(YML026C)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS24A(YER074W)|FD-Score:5.25|P-value:7.80E-8||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:SPO16(YHR153C)|FD-Score:4.35|P-value:6.75E-6||SGD DESC:Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation Gene:SSD1(YDR293C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:TRM10(YOL093W)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:tRNA methyltransferase; methylates the N-1 position of guanosine in tRNAs; protein abundance increases in response to DNA replication stress Gene:TRP1(YDR007W)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:YAL067W-A(YAL067W-A_p)|FD-Score:3.82|P-value:6.75E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDR056C(YDR056C_p)|FD-Score:3.16|P-value:7.93E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein Gene:YDR124W(YDR124W_p)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor Gene:YDR491C(YDR491C_d)|FD-Score:3.74|P-value:9.35E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL067C(YEL067C_p)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YER135C(YER135C_d)|FD-Score:-4|P-value:3.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YLR279W(YLR279W_d)|FD-Score:4.57|P-value:2.41E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR014C(YPR014C_d)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YSC84(YHR016C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication Gene:YVC1(YOR087W)|FD-Score:-3.37|P-value:3.71E-4||SGD DESC:Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR187C_d6.066.61E-100.92YDR187C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W
YNL290W5.151.34E-70.82RFC3Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YKL059C4.327.65E-60.16MPE1Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif
YDR188W4.161.58E-50.13CCT6Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif
YDR398W4.032.78E-50.13UTP5Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit
YLL050C3.904.72E-50.05COF1Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes
YBR152W3.855.92E-50.12SPP381mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p
YGL150C3.739.47E-50.12INO80ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription
YNR011C3.611.52E-40.02PRP2RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16
YGR083C3.591.62E-40.12GCD2Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YJR012C_p3.482.54E-40.22YJR012C_pEssential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W
YOR210W3.265.57E-40.15RPB10RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
YBL020W3.119.40E-40.04RFT1Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein
YHR020W3.070.001060.17YHR020WProlyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments
YER104W2.900.001870.05RTT105Protein with a role in regulation of Ty1 transposition

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIR023W12.201.98E-34DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YER074W5.257.80E-8RPS24AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication
YMR142C5.002.92E-7RPL13BRibosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication
YDR174W4.827.19E-7HMO1Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase
YAL019W4.592.21E-6FUN30Snf2p family member with ATP-dependent chromatin remodeling activity; has a role in silencing at the mating type locus, telomeres and centromeres; enriched at centromeres and is required for correct chromatin structure around centromeres, as well as at the boundary element of the silent HMR; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection of DSBs; potential Cdc28p substrate
YLR279W_d4.572.41E-6YLR279W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YHR153C4.356.75E-6SPO16Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation
YFL036W4.337.41E-6RPO41Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
YPR014C_d4.231.17E-5YPR014C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene
YMR242C3.953.89E-5RPL20ARibosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication
YAL067W-A_p3.826.75E-5YAL067W-A_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YDR491C_d3.749.35E-5YDR491C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDR293C3.651.32E-4SSD1Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function
YHR016C3.621.50E-4YSC84Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication
YBR007C_p3.591.66E-4DSF2_pDeletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress

GO enrichment analysis for SGTC_325
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2856.90E-111SGTC_486niguldipine 82.0 μMMiscellaneous12360.0721649amide catabolism
0.2531.23E-86SGTC_24605552655 174.2 μMMiscellaneous54019890.0571429amide catabolism
0.2466.83E-82SGTC_3371315-0038 354.0 μMChemDiv (Drug-like library)16264370.142857amide catabolism
0.2243.12E-68SGTC_28459003024 43.0 μMChembridge (Drug-like library)29901730.1amide catabolism
0.2015.56E-55SGTC_32289131799 49.5 μMChembridge (Drug-like library)170158180.138889amide catabolism
0.1946.87E-51SGTC_9903985-0011 41.3 μMChemDiv (Drug-like library)53995650.134328amide catabolism
0.1864.44E-47SGTC_5694130-2812 160.0 μMChemDiv (Drug-like library)7435520.133333amide catabolism
0.1791.56E-43SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.0757576
0.1714.51E-40SGTC_10563448-1962 115.0 μMChemDiv (Drug-like library)7155070.125amide catabolism
0.1673.25E-38SGTC_10583448-5425 97.3 μMChemDiv (Drug-like library)72797050.129032
0.1525.46E-32SGTC_11811222-0449 220.0 μMChemDiv (Drug-like library)6583180.180328
0.1505.05E-31SGTC_1864st057645 30.4 μMTimTec (Natural product derivative library)6886660.107692amide catabolism
0.1482.64E-30SGTC_1759st043059 70.0 μMTimTec (Natural product derivative library)6694260.0714286amide catabolism
0.1461.12E-29SGTC_7003453-2283 142.0 μMChemDiv (Drug-like library)5656640.123077amide catabolism
0.1423.98E-28SGTC_31679103034 49.5 μMChembridge (Drug-like library)51278590.0857143RPP1 & pyrimidine depletion

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2343799071614.84 μM0.5319156456862Chembridge (Fragment library)238.284462.87213
SGTC_21525568786200 μM0.43755399883Chembridge (Fragment library)226.23071.76424
SGTC_21285255449168.49 μM0.384615736008Chembridge (Fragment library)211.219361.38314
SGTC_930109-004559.23 μM0.38297988733ChemDiv (Drug-like library)210.23132.53413DNA damage response
SGTC_15130337-055919 μM0.367347775073ChemDiv (Drug-like library)211.216063.03813
SGTC_6841140-0066328 μM0.3661976813534ChemDiv (Drug-like library)406.861564.73715Golgi
SGTC_12131315-0166115 μM0.362069768401ChemDiv (Drug-like library)266.294562.88713
SGTC_5860957-02658.15 μM0.3571436752309ChemDiv (Drug-like library)388.41592.83726cell wall
SGTC_3542783-35004.88 μM0.3518526740761ChemDiv (Drug-like library)254.283862.28624cell wall
SGTC_7192810-423027.3 μM0.333333721079ChemDiv (Drug-like library)279.121423.86213