9135871

N-butyl-4-oxo-1H-quinazoline-2-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3252
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 17682838
SMILES CCCCNC(=O)C1=NC(=O)C2=CC=CC=C2N1
Standardized SMILES CCCCNC(=O)c1nc(O)c2ccccc2n1
Molecular weight 245.2771
ALogP 1.51
H-bond donor count 2
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.69
% growth inhibition (Hom. pool) -1.4


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17682838
Download HIP data (tab-delimited text)  (excel)
Gene:DUT1(YBR252W)|FD-Score:3.4|P-value:3.38E-4|Clearance:0.06||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:DYS1(YHR068W)|FD-Score:5.35|P-value:4.47E-8|Clearance:0.63||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:IRR1(YIL026C)|FD-Score:3.34|P-value:4.22E-4|Clearance:0.27||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:NUS1(YDL193W)|FD-Score:4.72|P-value:1.19E-6|Clearance:0.27||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PKC1(YBL105C)|FD-Score:3.42|P-value:3.10E-4|Clearance:0.02||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRP43(YGL120C)|FD-Score:-3.3|P-value:4.77E-4|Clearance:0||SGD DESC:RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome Gene:RER2(YBR002C)|FD-Score:4.04|P-value:2.67E-5|Clearance:0.44||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RET2(YFR051C)|FD-Score:-3.24|P-value:5.89E-4|Clearance:0||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPC17(YJL011C)|FD-Score:4.09|P-value:2.13E-5|Clearance:0.05||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPO26(YPR187W)|FD-Score:4.45|P-value:4.32E-6|Clearance:0.36||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPT5(YOR117W)|FD-Score:7.2|P-value:2.93E-13|Clearance:1.86||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:SMX2(YFL017W-A)|FD-Score:3.6|P-value:1.62E-4|Clearance:0.12||SGD DESC:Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G Gene:YJL086C(YJL086C_d)|FD-Score:3.48|P-value:2.54E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:DUT1(YBR252W)|FD-Score:3.4|P-value:3.38E-4|Clearance:0.06||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:DYS1(YHR068W)|FD-Score:5.35|P-value:4.47E-8|Clearance:0.63||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:IRR1(YIL026C)|FD-Score:3.34|P-value:4.22E-4|Clearance:0.27||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:NUS1(YDL193W)|FD-Score:4.72|P-value:1.19E-6|Clearance:0.27||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PKC1(YBL105C)|FD-Score:3.42|P-value:3.10E-4|Clearance:0.02||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRP43(YGL120C)|FD-Score:-3.3|P-value:4.77E-4|Clearance:0||SGD DESC:RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome Gene:RER2(YBR002C)|FD-Score:4.04|P-value:2.67E-5|Clearance:0.44||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RET2(YFR051C)|FD-Score:-3.24|P-value:5.89E-4|Clearance:0||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPC17(YJL011C)|FD-Score:4.09|P-value:2.13E-5|Clearance:0.05||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPO26(YPR187W)|FD-Score:4.45|P-value:4.32E-6|Clearance:0.36||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPT5(YOR117W)|FD-Score:7.2|P-value:2.93E-13|Clearance:1.86||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:SMX2(YFL017W-A)|FD-Score:3.6|P-value:1.62E-4|Clearance:0.12||SGD DESC:Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G Gene:YJL086C(YJL086C_d)|FD-Score:3.48|P-value:2.54E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17682838
Download HOP data (tab-delimited text)  (excel)
Gene:AEP2(YMR282C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AGE2(YIL044C)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif Gene:ASG1(YIL130W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Zinc cluster protein proposed to function as a transcriptional regulator involved in the stress response; null mutants have a respiratory deficiency, calcofluor white sensitivity and slightly increased cycloheximide resistance Gene:CEM1(YER061C)|FD-Score:3.89|P-value:4.95E-5||SGD DESC:Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration Gene:CHS3(YBR023C)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:COG7(YGL005C)|FD-Score:-3.18|P-value:7.42E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRP1(YHR146W)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Protein that binds to cruciform DNA structures Gene:CTK1(YKL139W)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:DSE3(YOR264W)|FD-Score:5.1|P-value:1.73E-7||SGD DESC:Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ECL1(YGR146C)|FD-Score:4.31|P-value:8.18E-6||SGD DESC:Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:ERG3(YLR056W)|FD-Score:-3.19|P-value:7.08E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:ERP1(YAR002C-A)|FD-Score:-3.86|P-value:5.57E-5||SGD DESC:Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication Gene:FAA1(YOR317W)|FD-Score:-4.26|P-value:1.02E-5||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FET4(YMR319C)|FD-Score:6.19|P-value:3.10E-10||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GCV3(YAL044C)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:HNT1(YDL125C)|FD-Score:-3.35|P-value:4.09E-4||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:HTD2(YHR067W)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology Gene:ICE2(YIL090W)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells Gene:JJJ3(YJR097W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:KTR6(YPL053C)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication Gene:LRE1(YCL051W)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:MTC1(YJL123C)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); mtc1 is synthetically lethal with cdc13-1 Gene:MUP3(YHL036W)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NRT1(YOR071C)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity Gene:PDR16(YNL231C)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PDR8(YLR266C)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PTH1(YHR189W)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:RMA1(YKL132C)|FD-Score:-3.19|P-value:7.17E-4||SGD DESC:Putative dihydrofolate synthetase; has similarity to Fol3p and to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RPS12(YOR369C)|FD-Score:4.15|P-value:1.70E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog Gene:SHU1(YHL006C)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SIR1(YKR101W)|FD-Score:4.46|P-value:4.06E-6||SGD DESC:Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin Gene:SRV2(YNL138W)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis Gene:TAT1(YBR069C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TEA1(YOR337W)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Ty1 enhancer activator required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein Gene:TIP41(YPR040W)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:VPS3(YDR495C)|FD-Score:4.46|P-value:4.14E-6||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:YDR008C(YDR008C_d)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR179W-A(YDR179W-A_p)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Putative protein of unknown function Gene:YDR509W(YDR509W_d)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL020C(YEL020C_p)|FD-Score:3.81|P-value:7.01E-5||SGD DESC:Protein of unknown function with low sequence identity to Pdc1p; mRNA identified as translated by ribosome profiling data Gene:YFR020W(YFR020W_p)|FD-Score:3.79|P-value:7.60E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGR054W(YGR054W)|FD-Score:5.44|P-value:2.62E-8||SGD DESC:Eukaryotic initiation factor (eIF) 2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A Gene:YIL096C(YIL096C_p)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis Gene:YJR018W(YJR018W_d)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR142W(YJR142W_p)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member Gene:YKR018C(YKR018C_p)|FD-Score:-4.42|P-value:4.95E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YPR098C(YPR098C)|FD-Score:-3.97|P-value:3.58E-5||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:AEP2(YMR282C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AGE2(YIL044C)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif Gene:ASG1(YIL130W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Zinc cluster protein proposed to function as a transcriptional regulator involved in the stress response; null mutants have a respiratory deficiency, calcofluor white sensitivity and slightly increased cycloheximide resistance Gene:CEM1(YER061C)|FD-Score:3.89|P-value:4.95E-5||SGD DESC:Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration Gene:CHS3(YBR023C)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:COG7(YGL005C)|FD-Score:-3.18|P-value:7.42E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRP1(YHR146W)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Protein that binds to cruciform DNA structures Gene:CTK1(YKL139W)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:DSE3(YOR264W)|FD-Score:5.1|P-value:1.73E-7||SGD DESC:Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ECL1(YGR146C)|FD-Score:4.31|P-value:8.18E-6||SGD DESC:Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:ERG3(YLR056W)|FD-Score:-3.19|P-value:7.08E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:ERP1(YAR002C-A)|FD-Score:-3.86|P-value:5.57E-5||SGD DESC:Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication Gene:FAA1(YOR317W)|FD-Score:-4.26|P-value:1.02E-5||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FET4(YMR319C)|FD-Score:6.19|P-value:3.10E-10||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GCV3(YAL044C)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:HNT1(YDL125C)|FD-Score:-3.35|P-value:4.09E-4||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:HTD2(YHR067W)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology Gene:ICE2(YIL090W)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells Gene:JJJ3(YJR097W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:KTR6(YPL053C)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication Gene:LRE1(YCL051W)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:MTC1(YJL123C)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); mtc1 is synthetically lethal with cdc13-1 Gene:MUP3(YHL036W)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NRT1(YOR071C)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity Gene:PDR16(YNL231C)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PDR8(YLR266C)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PTH1(YHR189W)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:RMA1(YKL132C)|FD-Score:-3.19|P-value:7.17E-4||SGD DESC:Putative dihydrofolate synthetase; has similarity to Fol3p and to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RPS12(YOR369C)|FD-Score:4.15|P-value:1.70E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog Gene:SHU1(YHL006C)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SIR1(YKR101W)|FD-Score:4.46|P-value:4.06E-6||SGD DESC:Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin Gene:SRV2(YNL138W)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis Gene:TAT1(YBR069C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TEA1(YOR337W)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Ty1 enhancer activator required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein Gene:TIP41(YPR040W)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:VPS3(YDR495C)|FD-Score:4.46|P-value:4.14E-6||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:YDR008C(YDR008C_d)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR179W-A(YDR179W-A_p)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Putative protein of unknown function Gene:YDR509W(YDR509W_d)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL020C(YEL020C_p)|FD-Score:3.81|P-value:7.01E-5||SGD DESC:Protein of unknown function with low sequence identity to Pdc1p; mRNA identified as translated by ribosome profiling data Gene:YFR020W(YFR020W_p)|FD-Score:3.79|P-value:7.60E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGR054W(YGR054W)|FD-Score:5.44|P-value:2.62E-8||SGD DESC:Eukaryotic initiation factor (eIF) 2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A Gene:YIL096C(YIL096C_p)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis Gene:YJR018W(YJR018W_d)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR142W(YJR142W_p)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member Gene:YKR018C(YKR018C_p)|FD-Score:-4.42|P-value:4.95E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YPR098C(YPR098C)|FD-Score:-3.97|P-value:3.58E-5||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR117W7.202.93E-131.86RPT5One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
YHR068W5.354.47E-80.63DYS1Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric
YDL193W4.721.19E-60.27NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY
YPR187W4.454.32E-60.35RPO26RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit
YJL011C4.092.13E-50.05RPC17RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress
YBR002C4.042.67E-50.44RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YFL017W-A3.601.62E-40.12SMX2Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G
YJL086C_d3.482.54E-40.05YJL086C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1
YBL105C3.423.10E-40.02PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YBR252W3.403.38E-40.06DUT1deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate
YIL026C3.344.22E-40.27IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YNL061W3.070.001070.07NOP2Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles
YPL169C3.000.001340.04MEX67Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
YER013W2.960.001520.00PRP22DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
YDR404C2.960.001520.10RPB7RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR319C6.193.10E-10FET4Low-affinity Fe(II) transporter of the plasma membrane
YGR054W5.442.62E-8YGR054WEukaryotic initiation factor (eIF) 2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A
YOR264W5.101.73E-7DSE3Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress
YHR189W4.691.38E-6PTH1One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium
YKR101W4.464.06E-6SIR1Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin
YDR495C4.464.14E-6VPS3Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
YGR146C4.318.18E-6ECL1Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation
YOR369C4.151.70E-5RPS12Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog
YJL123C4.062.44E-5MTC1Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); mtc1 is synthetically lethal with cdc13-1
YBR023C3.993.28E-5CHS3Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
YAL044C3.934.22E-5GCV3H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF
YER061C3.894.95E-5CEM1Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration
YDR509W_d3.865.66E-5YDR509W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YEL020C_p3.817.01E-5YEL020C_pProtein of unknown function with low sequence identity to Pdc1p; mRNA identified as translated by ribosome profiling data
YFR020W_p3.797.60E-5YFR020W_pProtein of unknown function; mRNA identified as translated by ribosome profiling data

GO enrichment analysis for SGTC_3252
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1057.96E-16SGTC_2129girgensonine 121.1 μMChembridge (Fragment library)4426380.046875heme biosynthesis & mitochondrial translocase
0.1032.12E-15SGTC_2231micatex 6.9 μMMiscellaneous207370.0545455
0.0985.78E-14SGTC_23369001819 198.7 μMChembridge (Fragment library)29896020.152542heme biosynthesis & mitochondrial translocase
0.0943.94E-13SGTC_14780453-0656 35.7 μMChemDiv (Drug-like library)52584960.134328
0.0945.11E-13SGTC_190279-0214 38.4 μMChemDiv (Drug-like library)35662740.0666667TRP & mitochondrial translation
0.0831.78E-10SGTC_2743auranofin 4.3 μMMiscellaneous166676690.0821918RPP1 & pyrimidine depletion
0.0831.99E-10SGTC_31789107506 49.5 μMChembridge (Drug-like library)88971940.152778
0.0822.52E-10SGTC_100279-0212 42.6 μMChemDiv (Drug-like library)398510.103448TRP & mitochondrial translation
0.0823.25E-10SGTC_2513baicalein 25.6 μMMicrosource (Natural product library)52816050.107692calcium & mitochondrial duress
0.0764.55E-9SGTC_20645220758 200.0 μMChembridge (Fragment library)7885180.151515
0.0741.21E-8SGTC_23459052315 157.4 μMChembridge (Fragment library)64692650.267857
0.0741.53E-8SGTC_267212a-hydroxy-5-deoxydehydromunduserone 100.0 μMMicrosource (Natural product library)46208980.0649351
0.0732.05E-8SGTC_748act1.228 77.9 μMChemDiv (Drug-like library)44215050.0508475redox potentiating
0.0708.83E-8SGTC_9332914-0560 114.0 μMChemDiv (Drug-like library)4638870.0615385
0.0681.50E-7SGTC_7670929-0023 86.0 μMChemDiv (Drug-like library)12045630.169231TRP & mitochondrial translation

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_22297354935190 μM0.372549318112Chembridge (Fragment library)237.256641.74722RSC complex & mRNA processing
SGTC_1074carmofur3.8 μM0.3275862577NIH Clinical Collection257.2614431.12524exosome
SGTC_5931203-021861.7 μM0.3272733666956ChemDiv (Drug-like library)244.33213.65302
SGTC_6731082-0474110 μM0.3214293098046ChemDiv (Drug-like library)247.246621.8414RSC complex & mRNA processing
SGTC_229benomyl22.87 μM0.3174628780Miscellaneous290.317722.96524tubulin folding & SWR complex
SGTC_7313966-0296387 μM0.301587660956ChemDiv (Drug-like library)318.367582.44314
SGTC_22127123476200 μM0.2884621085715Chembridge (Fragment library)204.291382.85711
SGTC_21685727979200 μM0.283019695210Chembridge (Fragment library)206.26422.23603
SGTC_3281914061349.47 μM0.26865716013766Chembridge (Drug-like library)361.436923.12703RPP1 & pyrimidine depletion
SGTC_23459052315157.45 μM0.2678576469265Chembridge (Fragment library)193.24561.48922