9135987

3-methyl-5-oxo-N-(2-phenylethyl)-[1,3]thiazolo[3,2-a]pyrimidine-6-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3253
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 4182430
SMILES CC1=CSC2=NC=C(C(=O)N12)C(=O)NCCC3=CC=CC=C3
Standardized SMILES CC1=CSC2=NC=C(C(=O)NCCc3ccccc3)C(=O)N12
Molecular weight 313.3742
ALogP 1.47
H-bond donor count 1
H-bond acceptor count 4
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.69
% growth inhibition (Hom. pool) 2.69


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4182430
Download HIP data (tab-delimited text)  (excel)
Gene:CDC15(YAR019C)|FD-Score:-3.26|P-value:5.64E-4|Clearance:0||SGD DESC:Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress Gene:DRE2(YKR071C)|FD-Score:-3.77|P-value:8.21E-5|Clearance:0||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:HSF1(YGL073W)|FD-Score:-3.22|P-value:6.40E-4|Clearance:0||SGD DESC:Trimeric heat shock transcription factor, activates multiple genes in response to stresses that include hyperthermia; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; posttranslationally regulated Gene:IMP3(YHR148W)|FD-Score:-3.56|P-value:1.88E-4|Clearance:0||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:LSG1(YGL099W)|FD-Score:4.77|P-value:9.37E-7|Clearance:0.65||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MGE1(YOR232W)|FD-Score:-3.7|P-value:1.10E-4|Clearance:0||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:NMD3(YHR170W)|FD-Score:3.13|P-value:8.69E-4|Clearance:0.01||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOC4(YPR144C)|FD-Score:-3.82|P-value:6.63E-5|Clearance:0||SGD DESC:Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits Gene:PMI40(YER003C)|FD-Score:4.4|P-value:5.50E-6|Clearance:0.65||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:RER2(YBR002C)|FD-Score:3.75|P-value:8.82E-5|Clearance:0.47||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RFA3(YJL173C)|FD-Score:-3.11|P-value:9.48E-4|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:SPC25(YER018C)|FD-Score:4.74|P-value:1.09E-6|Clearance:0.65||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:YGR190C(YGR190C_d)|FD-Score:3.13|P-value:8.86E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W Gene:YIF1(YNL263C)|FD-Score:3.28|P-value:5.26E-4|Clearance:0.14||SGD DESC:Integral membrane protein required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3 Gene:CDC15(YAR019C)|FD-Score:-3.26|P-value:5.64E-4|Clearance:0||SGD DESC:Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress Gene:DRE2(YKR071C)|FD-Score:-3.77|P-value:8.21E-5|Clearance:0||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:HSF1(YGL073W)|FD-Score:-3.22|P-value:6.40E-4|Clearance:0||SGD DESC:Trimeric heat shock transcription factor, activates multiple genes in response to stresses that include hyperthermia; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; posttranslationally regulated Gene:IMP3(YHR148W)|FD-Score:-3.56|P-value:1.88E-4|Clearance:0||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:LSG1(YGL099W)|FD-Score:4.77|P-value:9.37E-7|Clearance:0.65||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MGE1(YOR232W)|FD-Score:-3.7|P-value:1.10E-4|Clearance:0||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:NMD3(YHR170W)|FD-Score:3.13|P-value:8.69E-4|Clearance:0.01||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOC4(YPR144C)|FD-Score:-3.82|P-value:6.63E-5|Clearance:0||SGD DESC:Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits Gene:PMI40(YER003C)|FD-Score:4.4|P-value:5.50E-6|Clearance:0.65||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:RER2(YBR002C)|FD-Score:3.75|P-value:8.82E-5|Clearance:0.47||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RFA3(YJL173C)|FD-Score:-3.11|P-value:9.48E-4|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:SPC25(YER018C)|FD-Score:4.74|P-value:1.09E-6|Clearance:0.65||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:YGR190C(YGR190C_d)|FD-Score:3.13|P-value:8.86E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W Gene:YIF1(YNL263C)|FD-Score:3.28|P-value:5.26E-4|Clearance:0.14||SGD DESC:Integral membrane protein required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4182430
Download HOP data (tab-delimited text)  (excel)
Gene:AIM1(YAL046C)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:Protein involved in mitochondrial function or organization; null mutant displays elevated frequency of mitochondrial genome loss Gene:BTS1(YPL069C)|FD-Score:-6.04|P-value:7.92E-10||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CHS6(YJL099W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:FAR11(YNL127W)|FD-Score:-3.52|P-value:2.14E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:GOT1(YMR292W)|FD-Score:3.85|P-value:5.83E-5||SGD DESC:Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition Gene:GPT2(YKR067W)|FD-Score:3.94|P-value:4.09E-5||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:HTZ1(YOL012C)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:IRC11(YOR013W_d)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Dubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci Gene:IZH4(YOL101C)|FD-Score:4.57|P-value:2.38E-6||SGD DESC:Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress Gene:KTR2(YKR061W)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication Gene:LAP3(YNL239W)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:MAD3(YJL013C)|FD-Score:-3.56|P-value:1.85E-4||SGD DESC:Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover Gene:MDV1(YJL112W)|FD-Score:5.4|P-value:3.41E-8||SGD DESC:Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication Gene:MFM1(YPL060W)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MID2(YLR332W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:PDR17(YNL264C)|FD-Score:-3.83|P-value:6.51E-5||SGD DESC:Phosphatidylinositol transfer protein (PITP), downregulates Plb1p-mediated turnover of phosphatidylcholine, found in the cytosol and microsomes, homologous to Pdr16p, deletion affects phospholipid composition Gene:PIN2(YOR104W)|FD-Score:-4.12|P-value:1.86E-5||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated Gene:PUT2(YHR037W)|FD-Score:4.75|P-value:1.00E-6||SGD DESC:Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism Gene:SER2(YGR208W)|FD-Score:3.9|P-value:4.72E-5||SGD DESC:Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source Gene:SGE1(YPR198W)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SKY1(YMR216C)|FD-Score:5.44|P-value:2.72E-8||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SNF7(YLR025W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:STP22(YCL008C)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SWC3(YAL011W)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:TAT1(YBR069C)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDA11(YHR159W_p)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:TRP3(YKL211C)|FD-Score:-3.88|P-value:5.17E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.73|P-value:9.71E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TUB3(YML124C)|FD-Score:5.17|P-value:1.16E-7||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:VPS4(YPR173C)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:VPS71(YML041C)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBR184W(YBR184W_p)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Putative protein of unknown function; YBR184W is not an essential gene Gene:YCR090C(YCR090C_p)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene Gene:YDR090C(YDR090C_p)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:Putative protein of unknown function Gene:YDR509W(YDR509W_d)|FD-Score:3.99|P-value:3.33E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR069W(YGR069W_d)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR210C(YGR210C_p)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGR290W(YGR290W_d)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YLL006W-A(YLL006W-A_p)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YMR084W(YMR084W_p)|FD-Score:3.77|P-value:8.01E-5||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR087W(YMR087W)|FD-Score:4.74|P-value:1.08E-6||SGD DESC:Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain Gene:YNL195C(YNL195C_p)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR342C(YOR342C_p)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication Gene:YPR197C(YPR197C_d)|FD-Score:3.87|P-value:5.46E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:AIM1(YAL046C)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:Protein involved in mitochondrial function or organization; null mutant displays elevated frequency of mitochondrial genome loss Gene:BTS1(YPL069C)|FD-Score:-6.04|P-value:7.92E-10||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CHS6(YJL099W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:FAR11(YNL127W)|FD-Score:-3.52|P-value:2.14E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:GOT1(YMR292W)|FD-Score:3.85|P-value:5.83E-5||SGD DESC:Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition Gene:GPT2(YKR067W)|FD-Score:3.94|P-value:4.09E-5||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:HTZ1(YOL012C)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:IRC11(YOR013W_d)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Dubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci Gene:IZH4(YOL101C)|FD-Score:4.57|P-value:2.38E-6||SGD DESC:Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress Gene:KTR2(YKR061W)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication Gene:LAP3(YNL239W)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:MAD3(YJL013C)|FD-Score:-3.56|P-value:1.85E-4||SGD DESC:Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover Gene:MDV1(YJL112W)|FD-Score:5.4|P-value:3.41E-8||SGD DESC:Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication Gene:MFM1(YPL060W)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MID2(YLR332W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:PDR17(YNL264C)|FD-Score:-3.83|P-value:6.51E-5||SGD DESC:Phosphatidylinositol transfer protein (PITP), downregulates Plb1p-mediated turnover of phosphatidylcholine, found in the cytosol and microsomes, homologous to Pdr16p, deletion affects phospholipid composition Gene:PIN2(YOR104W)|FD-Score:-4.12|P-value:1.86E-5||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated Gene:PUT2(YHR037W)|FD-Score:4.75|P-value:1.00E-6||SGD DESC:Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism Gene:SER2(YGR208W)|FD-Score:3.9|P-value:4.72E-5||SGD DESC:Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source Gene:SGE1(YPR198W)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SKY1(YMR216C)|FD-Score:5.44|P-value:2.72E-8||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SNF7(YLR025W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:STP22(YCL008C)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SWC3(YAL011W)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:TAT1(YBR069C)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDA11(YHR159W_p)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:TRP3(YKL211C)|FD-Score:-3.88|P-value:5.17E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.73|P-value:9.71E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TUB3(YML124C)|FD-Score:5.17|P-value:1.16E-7||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:VPS4(YPR173C)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:VPS71(YML041C)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBR184W(YBR184W_p)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Putative protein of unknown function; YBR184W is not an essential gene Gene:YCR090C(YCR090C_p)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene Gene:YDR090C(YDR090C_p)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:Putative protein of unknown function Gene:YDR509W(YDR509W_d)|FD-Score:3.99|P-value:3.33E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR069W(YGR069W_d)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR210C(YGR210C_p)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGR290W(YGR290W_d)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YLL006W-A(YLL006W-A_p)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YMR084W(YMR084W_p)|FD-Score:3.77|P-value:8.01E-5||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR087W(YMR087W)|FD-Score:4.74|P-value:1.08E-6||SGD DESC:Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain Gene:YNL195C(YNL195C_p)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR342C(YOR342C_p)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication Gene:YPR197C(YPR197C_d)|FD-Score:3.87|P-value:5.46E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W4.779.37E-70.65LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YER018C4.741.09E-60.65SPC25Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
YER003C4.405.50E-60.65PMI40Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
YBR002C3.758.82E-50.47RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YNL263C3.285.26E-40.14YIF1Integral membrane protein required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3
YHR170W3.138.69E-40.01NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YGR190C_d3.138.86E-40.06YGR190C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W
YDR086C3.060.001100.03SSS1Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p
YJR076C3.030.001200.07CDC11Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YIL051C2.960.001530.03MMF1Mitochondrial protein required for transamination of isoleucine but not of valine or leucine; may regulate specificity of branched-chain transaminases Bat1p and Bat2p; interacts genetically with mitochondrial ribosomal protein genes
YPL169C2.940.001660.02MEX67Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
YHL015W2.910.001780.08RPS20Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10
YDR327W_d2.840.002290.03YDR327W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1
YDL058W2.810.002480.06USO1Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
YKL036C_d2.750.003007.64E-5YKL036C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR216C5.442.72E-8SKY1SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1
YJL112W5.403.41E-8MDV1Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication
YML124C5.171.16E-7TUB3Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
YHR037W4.751.00E-6PUT2Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism
YMR087W4.741.08E-6YMR087WPutative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain
YOL101C4.572.38E-6IZH4Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress
YBR069C4.191.40E-5TAT1Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
YML041C4.151.69E-5VPS71Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
YOL012C4.052.61E-5HTZ1Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin
YDR509W_d3.993.33E-5YDR509W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YAL011W3.953.84E-5SWC3Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae
YKR067W3.944.09E-5GPT2Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen
YGR208W3.904.72E-5SER2Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source
YPR197C_d3.875.46E-5YPR197C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YMR292W3.855.83E-5GOT1Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition

GO enrichment analysis for SGTC_3253
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1441.19E-28SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.10810860S ribosome export
0.1382.54E-26SGTC_13261431-2094 10.8 μMChemDiv (Drug-like library)6162780.153846tubulin folding & SWR complex
0.1343.53E-25SGTC_23196033012 152.6 μMChembridge (Fragment library)8472460.161765tubulin folding & SWR complex
0.1291.76E-23SGTC_229benomyl 22.9 μMMiscellaneous287800.184211tubulin folding & SWR complex
0.1285.48E-23SGTC_286k060-0067 22.9 μMChemDiv (Drug-like library)59850510.0963855tubulin folding & SWR complex
0.1231.89E-21SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.10638360S ribosome export
0.1232.16E-21SGTC_13521496-0991 99.0 μMChemDiv (Drug-like library)30000390.134328tubulin folding & SWR complex
0.1202.03E-20SGTC_21745790901 200.0 μMChembridge (Fragment library)7860200.173913tubulin folding & SWR complex
0.1141.37E-18SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.090909160S ribosome export
0.1118.88E-18SGTC_1033k072-0102 21.3 μMChemDiv (Drug-like library)59659310.11688360S ribosome export
0.1088.50E-17SGTC_21415621638 7.7 μMChembridge (Fragment library)28562380.131579tubulin folding & SWR complex
0.1081.07E-16SGTC_468gf-109203x 121.0 μMICCB bioactive library23960.104651amide catabolism
0.1071.40E-16SGTC_8251988-2224 89.6 μMChemDiv (Drug-like library)11808590.120482cell wall signaling
0.1023.79E-15SGTC_23529027995 87.8 μMChembridge (Fragment library)29973470.111111tubulin folding & SWR complex
0.1016.36E-15SGTC_1971st069429 63.5 μMTimTec (Natural product derivative library)12465220.20547960S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3223912997049.47 μM0.39682517248386Chembridge (Drug-like library)327.400782.62614
SGTC_3242913394049.47 μM0.37313417248444Chembridge (Drug-like library)305.395261.94414
SGTC_2888905854712.99 μM0.33333316476514Chembridge (Drug-like library)279.333084.11812
SGTC_6530203-001655.2 μM0.306452295302ChemDiv (Drug-like library)289.756743.44212
SGTC_3217912967349.47 μM0.29850717248312Chembridge (Drug-like library)305.395261.8214
SGTC_6681682-049962.6 μM0.287879682214ChemDiv (Drug-like library)324.20184.10612
SGTC_23619034121116 μM0.2786896496917Chembridge (Fragment library)228.269721.3603RSC complex & mRNA processing
SGTC_3260913710949.47 μM0.27419417478346Chembridge (Drug-like library)255.311683.1561260S ribosome export
SGTC_9061165-0455526 μM0.2622953252859ChemDiv (Drug-like library)304.364141.90224
SGTC_3256913890249.47 μM0.25757617228773Chembridge (Drug-like library)305.373663.7321PDR1