9138902

2-anilino-6-methyl-5-(2-phenylethyl)-1H-pyrimidin-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3256
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 17228773
SMILES CC1=C(C(=O)N=C(N1)NC2=CC=CC=C2)CCC3=CC=CC=C3
Standardized SMILES Cc1nc(Nc2ccccc2)nc(O)c1CCc3ccccc3
Molecular weight 305.3737
ALogP 3.73
H-bond donor count 2
H-bond acceptor count 1
Response signature PDR1

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.88
% growth inhibition (Hom. pool) -1.49


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17228773
Download HIP data (tab-delimited text)  (excel)
Gene:RIB7(YBR153W)|FD-Score:-3.15|P-value:8.04E-4|Clearance:0||SGD DESC:Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway Gene:YCL041C(YCL041C_d)|FD-Score:3.2|P-value:6.83E-4|Clearance:0.25||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W Gene:RIB7(YBR153W)|FD-Score:-3.15|P-value:8.04E-4|Clearance:0||SGD DESC:Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway Gene:YCL041C(YCL041C_d)|FD-Score:3.2|P-value:6.83E-4|Clearance:0.25||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17228773
Download HOP data (tab-delimited text)  (excel)
Gene:GEF1(YJR040W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism Gene:HXK2(YGL253W)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication Gene:PDR1(YGL013C)|FD-Score:3.75|P-value:8.76E-5||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:QRI5(YLR204W)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:TCB1(YOR086C)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:YGR011W(YGR011W_d)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL338W(YNL338W_d)|FD-Score:4.01|P-value:3.06E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps TEL14L-XC, which is Telomeric X element Core sequence on the left arm of Chromosome XIV Gene:YOL073C(YOL073C_p)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; ortholog of fission yeast dsc2 Gene:GEF1(YJR040W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism Gene:HXK2(YGL253W)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication Gene:PDR1(YGL013C)|FD-Score:3.75|P-value:8.76E-5||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:QRI5(YLR204W)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:TCB1(YOR086C)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:YGR011W(YGR011W_d)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL338W(YNL338W_d)|FD-Score:4.01|P-value:3.06E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps TEL14L-XC, which is Telomeric X element Core sequence on the left arm of Chromosome XIV Gene:YOL073C(YOL073C_p)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; ortholog of fission yeast dsc2

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YCL041C_d3.206.83E-40.25YCL041C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W
YER168C2.970.001490.25CCA1ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites
YBL026W2.930.001720.25LSM2Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YLR355C2.910.001810.25ILV5Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
YKL088W2.660.003950.06CAB3Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC
YKL182W2.600.004700.07FAS1Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
YKL173W2.530.005710.05SNU114GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2
YHR107C2.480.006500.11CDC12Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YHR024C2.380.008710.01MAS2Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
YPR165W2.360.009049.02E-5RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YAR008W2.360.009040.07SEN34Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease
YKR062W2.290.011000.03TFA2TFIIE small subunit, involved in RNA polymerase II transcription initiation
YOR232W2.260.012000.02MGE1Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE
YGL065C2.230.012700.03ALG2Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol
YKL095W2.210.013600.01YJU2Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL338W_d4.013.06E-5YNL338W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps TEL14L-XC, which is Telomeric X element Core sequence on the left arm of Chromosome XIV
YGL013C3.758.76E-5PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YGR011W_d3.641.39E-4YGR011W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YJR040W3.472.57E-4GEF1Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism
YLR204W3.246.00E-4QRI5Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA
YOL073C_p3.187.33E-4YOL073C_pSubunit of the DSC ubiquitin ligase complex; ortholog of fission yeast dsc2
YGL253W3.187.46E-4HXK2Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication
YKL201C3.040.00119MNN4Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication
YHR048W_p2.960.00153YHK8_pPresumed antiporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles
YDR451C2.940.00165YHP1Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication
YGR108W2.880.00202CLB1B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome
YGR050C_p2.870.00208YGR050C_pProtein of unknown function; mRNA identified as translated by ribosome profiling data
YPL015C2.850.00221HST2Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export
YER180C2.800.00257ISC10Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells
YDR105C2.740.00307TMS1Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance

GO enrichment analysis for SGTC_3256
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2343.50E-74SGTC_32419133421 49.5 μMChembridge (Drug-like library)170182210.0823529PDR1
0.2033.22E-56SGTC_5274476-4215 268.0 μMChemDiv (Drug-like library)44187540.0777778PDR1
0.2003.95E-54SGTC_12770873-0036 22.0 μMChemDiv (Drug-like library)59657350.0704225PDR1
0.1979.28E-53SGTC_295k015-0027 31.0 μMChemDiv (Drug-like library)38874240.134328PDR1
0.1951.74E-51SGTC_1700st030840 7.9 μMTimTec (Natural product derivative library)54993070.0930233PDR1
0.1942.17E-51SGTC_12430416-0003 5.7 μMChemDiv (Drug-like library)21419770.0508475PDR1
0.1623.56E-36SGTC_250brefeldin a 552.2 μMICCB bioactive library64361870.0684932PDR1
0.1529.65E-32SGTC_1139k015-0024 46.9 μMChemDiv (Drug-like library)27666520.15625PDR1
0.1499.74E-31SGTC_6641222-0054 14.9 μMChemDiv (Drug-like library)68139910.117647sphingolipid biosynthesis & PDR1
0.1403.33E-27SGTC_30259087895 49.5 μMChembridge (Drug-like library)171736410.116883PDR1
0.1375.39E-26SGTC_2508celastrol 40.5 μMMicrosource (Natural product library)1227240.0705882PDR1
0.1322.45E-24SGTC_12870971-0001 34.6 μMChemDiv (Drug-like library)46658690.126984sphingolipid biosynthesis & PDR1
0.1293.32E-23SGTC_2752diclazuril 33.1 μMMiscellaneous4563890.109589
0.1284.26E-23SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.125ERG2
0.1263.28E-22SGTC_2416tofa 880.0 nMMiscellaneous1151750.09375sphingolipid biosynthesis & PDR1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_22977971671152.32 μM0.293103909016Chembridge (Fragment library)256.259981.39323RNA processing & uracil transport
SGTC_2351902685872.31 μM0.2777781409300Chembridge (Fragment library)231.250520.87923RNA processing & uracil transport
SGTC_20825219693150 μM0.2727272834649Chembridge (Fragment library)236.228820.44424RNA processing & uracil transport
SGTC_3171910319249.47 μM0.27118613018434Chembridge (Drug-like library)269.338263.50512
SGTC_3207911416349.47 μM0.271186849233Chembridge (Drug-like library)269.338263.43812
SGTC_13261431-209410.8 μM0.269231616278ChemDiv (Drug-like library)234.295764.59112tubulin folding & SWR complex
SGTC_9841492-02552.91 μM0.2678571585198ChemDiv (Drug-like library)344.452726.12813SWF1 & branched chain AA biosynthesis
SGTC_2839900395125.97 μM0.2666672990634Chembridge (Drug-like library)283.364843.81512
SGTC_21755807670165.31 μM0.259259241186Chembridge (Fragment library)218.27492.03713
SGTC_23427990637118.86 μM0.2586212033102Chembridge (Fragment library)242.23341.07123RNA processing & uracil transport