9139206

5-methyl-3-(4-methylphenyl)-7-(4-methylpiperazin-1-yl)pyrazolo[1,5-a]pyrimidine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3257
Screen concentration 33.0 μM
Source Chembridge (Drug-like library)
PubChem CID 4910041
SMILES CC1=CC=C(C=C1)C2=C3N=C(C=C(N3N=C2)N4CCN(CC4)C)C
Standardized SMILES CN1CCN(CC1)c2cc(C)nc3c(cnn23)c4ccc(C)cc4
Molecular weight 321.4194
ALogP 3.05
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.81
% growth inhibition (Hom. pool) -0.18


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4910041
Download HIP data (tab-delimited text)  (excel)
Gene:BBP1(YPL255W)|FD-Score:3.26|P-value:5.52E-4|Clearance:0.15||SGD DESC:Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p Gene:ERD2(YBL040C)|FD-Score:3.76|P-value:8.52E-5|Clearance:0.07||SGD DESC:HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins Gene:LSG1(YGL099W)|FD-Score:4.59|P-value:2.18E-6|Clearance:0.83||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MES1(YGR264C)|FD-Score:5.67|P-value:7.31E-9|Clearance:0.83||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:NMD3(YHR170W)|FD-Score:3.53|P-value:2.04E-4|Clearance:0.11||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOC2(YOR206W)|FD-Score:3.37|P-value:3.70E-4|Clearance:0.11||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NOP53(YPL146C)|FD-Score:3.63|P-value:1.41E-4|Clearance:0.1||SGD DESC:Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired Gene:PDS1(YDR113C)|FD-Score:-3.78|P-value:7.82E-5|Clearance:0||SGD DESC:Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:PRP24(YMR268C)|FD-Score:3.63|P-value:1.39E-4|Clearance:0||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:PRP45(YAL032C)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.06||SGD DESC:Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene Gene:RIX1(YHR197W)|FD-Score:4.69|P-value:1.37E-6|Clearance:0.83||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:SEC9(YGR009C)|FD-Score:3.43|P-value:3.07E-4|Clearance:0.05||SGD DESC:t-SNARE protein important for fusion of secretory vesicles with the plasma membrane; similar to but not functionally redundant with Spo20p; SNAP-25 homolog Gene:SED5(YLR026C)|FD-Score:4.86|P-value:5.99E-7|Clearance:0.83||SGD DESC:cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins Gene:YAL034C-B(YAL034C-B_d)|FD-Score:3.11|P-value:9.43E-4|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKL153W(YKL153W_d)|FD-Score:-3.22|P-value:6.38E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant Gene:BBP1(YPL255W)|FD-Score:3.26|P-value:5.52E-4|Clearance:0.15||SGD DESC:Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p Gene:ERD2(YBL040C)|FD-Score:3.76|P-value:8.52E-5|Clearance:0.07||SGD DESC:HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins Gene:LSG1(YGL099W)|FD-Score:4.59|P-value:2.18E-6|Clearance:0.83||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MES1(YGR264C)|FD-Score:5.67|P-value:7.31E-9|Clearance:0.83||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:NMD3(YHR170W)|FD-Score:3.53|P-value:2.04E-4|Clearance:0.11||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOC2(YOR206W)|FD-Score:3.37|P-value:3.70E-4|Clearance:0.11||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NOP53(YPL146C)|FD-Score:3.63|P-value:1.41E-4|Clearance:0.1||SGD DESC:Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired Gene:PDS1(YDR113C)|FD-Score:-3.78|P-value:7.82E-5|Clearance:0||SGD DESC:Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:PRP24(YMR268C)|FD-Score:3.63|P-value:1.39E-4|Clearance:0||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:PRP45(YAL032C)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.06||SGD DESC:Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene Gene:RIX1(YHR197W)|FD-Score:4.69|P-value:1.37E-6|Clearance:0.83||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:SEC9(YGR009C)|FD-Score:3.43|P-value:3.07E-4|Clearance:0.05||SGD DESC:t-SNARE protein important for fusion of secretory vesicles with the plasma membrane; similar to but not functionally redundant with Spo20p; SNAP-25 homolog Gene:SED5(YLR026C)|FD-Score:4.86|P-value:5.99E-7|Clearance:0.83||SGD DESC:cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins Gene:YAL034C-B(YAL034C-B_d)|FD-Score:3.11|P-value:9.43E-4|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKL153W(YKL153W_d)|FD-Score:-3.22|P-value:6.38E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4910041
Download HOP data (tab-delimited text)  (excel)
Gene:ANT1(YPR128C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:ARF2(YDL137W)|FD-Score:3.8|P-value:7.34E-5||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication Gene:CNB1(YKL190W)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:COA3(YJL062W-A)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Mitochondrial inner membrane protein that participates in regulation of COX1 translation, Cox1p stabilization, and cytochrome oxidase assembly Gene:CRG1(YHR209W)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:CYS4(YGR155W)|FD-Score:-3.12|P-value:9.03E-4||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:ELM1(YKL048C)|FD-Score:4.52|P-value:3.10E-6||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:EPS1(YIL005W)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:GCV2(YMR189W)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm Gene:MCM22(YJR135C)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4 Gene:MTF2(YDL044C)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:PUT3(YKL015W)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences as a dimer and undergoes a conformational change to form the active state; differentially phosphorylated in the presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain Gene:RDR1(YOR380W)|FD-Score:-3.22|P-value:6.51E-4||SGD DESC:Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins Gene:RGI1(YER067W)|FD-Score:-3.18|P-value:7.43E-4||SGD DESC:Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:RSM18(YER050C)|FD-Score:-3.21|P-value:6.72E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:SHU2(YDR078C)|FD-Score:3.83|P-value:6.28E-5||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SNF2(YOR290C)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:TRS33(YOR115C)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:VPS1(YKR001C)|FD-Score:-3.24|P-value:5.98E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS70(YJR126C)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Protein of unknown function involved in vacuolar protein sorting Gene:YDR132C(YDR132C_p)|FD-Score:4.02|P-value:2.95E-5||SGD DESC:Protein of unknown function; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; YDR132C has a paralog, YLR108C, that arose from the whole genome duplication Gene:YDR182W-A(YDR182W-A_p)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR209C(YDR209C_d)|FD-Score:-4.01|P-value:2.97E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YDR210W. Gene:YDR222W(YDR222W_p)|FD-Score:3.75|P-value:8.80E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YGL117W(YGL117W_p)|FD-Score:4.8|P-value:7.74E-7||SGD DESC:Putative protein of unknown function Gene:YGR111W(YGR111W_p)|FD-Score:3.77|P-value:8.04E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YKL169C(YKL169C_d)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YNL022C(YNL022C_p)|FD-Score:-3.44|P-value:2.86E-4||SGD DESC:Putative cytosine 5-methyltransferase, contains seven beta-strand methyltransferase motif similar to NOP2/YNL061W; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; predicted to be involved in ribosome biogenesis Gene:YNL190W(YNL190W_p)|FD-Score:-3.09|P-value:9.88E-4||SGD DESC:Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site Gene:YPR123C(YPR123C_d)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YTA12(YMR089C)|FD-Score:-4.16|P-value:1.60E-5||SGD DESC:Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes Gene:ANT1(YPR128C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:ARF2(YDL137W)|FD-Score:3.8|P-value:7.34E-5||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication Gene:CNB1(YKL190W)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:COA3(YJL062W-A)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Mitochondrial inner membrane protein that participates in regulation of COX1 translation, Cox1p stabilization, and cytochrome oxidase assembly Gene:CRG1(YHR209W)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:CYS4(YGR155W)|FD-Score:-3.12|P-value:9.03E-4||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:ELM1(YKL048C)|FD-Score:4.52|P-value:3.10E-6||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:EPS1(YIL005W)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:GCV2(YMR189W)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm Gene:MCM22(YJR135C)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4 Gene:MTF2(YDL044C)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:PUT3(YKL015W)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences as a dimer and undergoes a conformational change to form the active state; differentially phosphorylated in the presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain Gene:RDR1(YOR380W)|FD-Score:-3.22|P-value:6.51E-4||SGD DESC:Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins Gene:RGI1(YER067W)|FD-Score:-3.18|P-value:7.43E-4||SGD DESC:Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:RSM18(YER050C)|FD-Score:-3.21|P-value:6.72E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:SHU2(YDR078C)|FD-Score:3.83|P-value:6.28E-5||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SNF2(YOR290C)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:TRS33(YOR115C)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:VPS1(YKR001C)|FD-Score:-3.24|P-value:5.98E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS70(YJR126C)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Protein of unknown function involved in vacuolar protein sorting Gene:YDR132C(YDR132C_p)|FD-Score:4.02|P-value:2.95E-5||SGD DESC:Protein of unknown function; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; YDR132C has a paralog, YLR108C, that arose from the whole genome duplication Gene:YDR182W-A(YDR182W-A_p)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR209C(YDR209C_d)|FD-Score:-4.01|P-value:2.97E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YDR210W. Gene:YDR222W(YDR222W_p)|FD-Score:3.75|P-value:8.80E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YGL117W(YGL117W_p)|FD-Score:4.8|P-value:7.74E-7||SGD DESC:Putative protein of unknown function Gene:YGR111W(YGR111W_p)|FD-Score:3.77|P-value:8.04E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YKL169C(YKL169C_d)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YNL022C(YNL022C_p)|FD-Score:-3.44|P-value:2.86E-4||SGD DESC:Putative cytosine 5-methyltransferase, contains seven beta-strand methyltransferase motif similar to NOP2/YNL061W; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; predicted to be involved in ribosome biogenesis Gene:YNL190W(YNL190W_p)|FD-Score:-3.09|P-value:9.88E-4||SGD DESC:Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site Gene:YPR123C(YPR123C_d)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YTA12(YMR089C)|FD-Score:-4.16|P-value:1.60E-5||SGD DESC:Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR264C5.677.31E-90.83MES1Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs
YLR026C4.865.99E-70.83SED5cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins
YHR197W4.691.37E-60.83RIX1Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene
YGL099W4.592.18E-60.83LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YBL040C3.768.52E-50.07ERD2HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins
YAL032C3.691.12E-40.06PRP45Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene
YMR268C3.631.39E-40.00PRP24Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling
YPL146C3.631.41E-40.10NOP53Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired
YHR170W3.532.04E-40.11NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YGR009C3.433.07E-40.05SEC9t-SNARE protein important for fusion of secretory vesicles with the plasma membrane; similar to but not functionally redundant with Spo20p; SNAP-25 homolog
YOR206W3.373.70E-40.11NOC2Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors
YPL255W3.265.52E-40.15BBP1Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p
YAL034C-B_d3.119.43E-40.07YAL034C-B_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YLR230W_d3.030.001200.05YLR230W_dDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity
YMR260C2.990.001410.00TIF11Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL117W_p4.807.74E-7YGL117W_pPutative protein of unknown function
YKL048C4.523.10E-6ELM1Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring
YDR132C_p4.022.95E-5YDR132C_pProtein of unknown function; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; YDR132C has a paralog, YLR108C, that arose from the whole genome duplication
YDR078C3.836.28E-5SHU2Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function
YDL137W3.807.34E-5ARF2ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication
YGR111W_p3.778.04E-5YGR111W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
YDR222W_p3.758.80E-5YDR222W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
YPR123C_d3.571.78E-4YPR123C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR
YJR126C3.532.05E-4VPS70Protein of unknown function involved in vacuolar protein sorting
YJL062W-A3.512.20E-4COA3Mitochondrial inner membrane protein that participates in regulation of COX1 translation, Cox1p stabilization, and cytochrome oxidase assembly
YPR128C3.492.43E-4ANT1Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation
YDR182W-A_p3.482.55E-4YDR182W-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YOR115C3.334.39E-4TRS33One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
YDL044C3.304.78E-4MTF2Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription
YIL005W3.304.84E-4EPS1ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family

GO enrichment analysis for SGTC_3257
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1353.12E-25SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.064516160S ribosome export
0.1331.05E-24SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.097222260S ribosome export
0.1314.92E-24SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.088235360S ribosome export
0.1211.10E-20SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.14473760S ribosome export
0.1196.00E-20SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.069444460S ribosome export
0.1181.08E-19SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.093333360S ribosome export
0.1095.87E-17SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.16901460S ribosome export
0.1081.06E-16SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.10975660S ribosome export
0.0961.62E-13SGTC_1350933-0004 26.3 μMChemDiv (Drug-like library)212306170.066666760S ribosome export
0.0904.26E-12SGTC_32519135047 49.5 μMChembridge (Drug-like library)176829630.11392460S ribosome export
0.0895.90E-12SGTC_32769139060 49.5 μMChembridge (Drug-like library)48706190.16455760S ribosome export
0.0897.07E-12SGTC_490717-0965 32.1 μMChemDiv (Drug-like library)58893490.092105360S ribosome export
0.0897.89E-12SGTC_22067254556 147.0 μMChembridge (Fragment library)43989050.08450760S ribosome export
0.0898.88E-12SGTC_2731amoxapine 43.4 μMMiscellaneous21700.138889
0.0899.41E-12SGTC_21005377183 190.0 μMChembridge (Fragment library)28442020.11764760S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3349914970323 μM0.725494910494Chembridge (Drug-like library)351.44542.50915
SGTC_3328914062429.04 μM0.644904392Chembridge (Drug-like library)335.4463.33104
SGTC_3280914096149.47 μM0.4827594901226Chembridge (Drug-like library)355.864483.5090460S ribosome export
SGTC_3335914331529.63 μM0.476194899691Chembridge (Drug-like library)365.471982.62615fatty acid desaturase (OLE1)
SGTC_3222912830149.47 μM0.4745764911358Chembridge (Drug-like library)375.390813.9330760S ribosome export
SGTC_3316913663910.55 μM0.4516134903665Chembridge (Drug-like library)347.45673.5704
SGTC_334891494643.54 μM0.4444441951442Chembridge (Drug-like library)379.498563.56915
SGTC_3340914663411.1 μM0.43754907387Chembridge (Drug-like library)365.471983.14615
SGTC_3320913700535.14 μM0.3934434908713Chembridge (Drug-like library)335.4463.39704endomembrane recycling
SGTC_3235913260249.47 μM0.3870974912240Chembridge (Drug-like library)352.430163.31605