9138477

3-(2-methoxyphenyl)-5-methyl-N-(2-morpholin-4-ylethyl)pyrazolo[1,5-a]pyrimidin-7-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3258
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 4970355
SMILES CC1=NC2=C(C=NN2C(=C1)NCCN3CCOCC3)C4=CC=CC=C4OC
Standardized SMILES COc1ccccc1c2cnn3c(NCCN4CCOCC4)cc(C)nc23
Molecular weight 367.4448
ALogP 2.06
H-bond donor count 1
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0
% growth inhibition (Hom. pool) -2.98


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4970355
Download HIP data (tab-delimited text)  (excel)
Gene:RPA43(YOR340C)|FD-Score:3.88|P-value:5.29E-5|Clearance:0.84||SGD DESC:RNA polymerase I subunit A43 Gene:SED5(YLR026C)|FD-Score:3.84|P-value:6.15E-5|Clearance:0.84||SGD DESC:cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins Gene:RPA43(YOR340C)|FD-Score:3.88|P-value:5.29E-5|Clearance:0.84||SGD DESC:RNA polymerase I subunit A43 Gene:SED5(YLR026C)|FD-Score:3.84|P-value:6.15E-5|Clearance:0.84||SGD DESC:cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4970355
Download HOP data (tab-delimited text)  (excel)
Gene:ARL1(YBR164C)|FD-Score:-3.95|P-value:3.84E-5||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:CHS7(YHR142W)|FD-Score:-3.17|P-value:7.57E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:COG5(YNL051W)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DBP3(YGL078C)|FD-Score:3.76|P-value:8.38E-5||SGD DESC:RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype Gene:DNL4(YOR005C)|FD-Score:-3.1|P-value:9.71E-4||SGD DESC:DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth Gene:EHD3(YDR036C)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:3-hydroxyisobutyryl-CoA hydrolase, member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis Gene:ESC1(YMR219W)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Protein localized to the nuclear periphery, involved in telomeric silencing; interacts with PAD4-domain of Sir4p Gene:FAA1(YOR317W)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:GCR2(YNL199C)|FD-Score:-4.14|P-value:1.71E-5||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:HST2(YPL015C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:HXT12(YIL170W)|FD-Score:-3.18|P-value:7.27E-4||SGD DESC:Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family Gene:MCM22(YJR135C)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4 Gene:MPH1(YIR002C)|FD-Score:-3.95|P-value:3.87E-5||SGD DESC:3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases Gene:MRP2(YPR166C)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSD1(YPL104W)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:NMA111(YNL123W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NTO1(YPR031W)|FD-Score:4.36|P-value:6.65E-6||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:ROY1(YMR258C)|FD-Score:3.09|P-value:9.87E-4||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:SAY1(YGR263C)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:SIT4(YDL047W)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:TDA3(YHR009C_p)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:THO2(YNL139C)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids Gene:TPS2(YDR074W)|FD-Score:4.94|P-value:3.97E-7||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:TRP2(YER090W)|FD-Score:4.3|P-value:8.48E-6||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UME1(YPL139C)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p Gene:VAC14(YLR386W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VPS61(YDR136C_d)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YEL067C(YEL067C_p)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YGL218W(YGL218W_d)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance Gene:YHM2(YMR241W)|FD-Score:5.05|P-value:2.23E-7||SGD DESC:Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome Gene:YIL012W(YIL012W_d)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL086C(YIL086C_d)|FD-Score:3.13|P-value:8.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR366W(YLR366W_d)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A Gene:YML003W(YML003W_p)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Putative protein of unknown function Gene:YNR073C(YNR073C_p)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Putative mannitol dehydrogenase; identical in amino acid sequence to Dsf1p Gene:YOL073C(YOL073C_p)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; ortholog of fission yeast dsc2 Gene:YOR114W(YOR114W_p)|FD-Score:3.82|P-value:6.65E-5||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:YOR283W(YOR283W)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene Gene:YPR098C(YPR098C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YPT31(YER031C)|FD-Score:-3.42|P-value:3.09E-4||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:ZIP2(YGL249W)|FD-Score:-3.88|P-value:5.32E-5||SGD DESC:Meiosis-specific protein; involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:ARL1(YBR164C)|FD-Score:-3.95|P-value:3.84E-5||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:CHS7(YHR142W)|FD-Score:-3.17|P-value:7.57E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:COG5(YNL051W)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DBP3(YGL078C)|FD-Score:3.76|P-value:8.38E-5||SGD DESC:RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype Gene:DNL4(YOR005C)|FD-Score:-3.1|P-value:9.71E-4||SGD DESC:DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth Gene:EHD3(YDR036C)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:3-hydroxyisobutyryl-CoA hydrolase, member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis Gene:ESC1(YMR219W)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Protein localized to the nuclear periphery, involved in telomeric silencing; interacts with PAD4-domain of Sir4p Gene:FAA1(YOR317W)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:GCR2(YNL199C)|FD-Score:-4.14|P-value:1.71E-5||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:HST2(YPL015C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:HXT12(YIL170W)|FD-Score:-3.18|P-value:7.27E-4||SGD DESC:Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family Gene:MCM22(YJR135C)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4 Gene:MPH1(YIR002C)|FD-Score:-3.95|P-value:3.87E-5||SGD DESC:3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases Gene:MRP2(YPR166C)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSD1(YPL104W)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:NMA111(YNL123W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NTO1(YPR031W)|FD-Score:4.36|P-value:6.65E-6||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:ROY1(YMR258C)|FD-Score:3.09|P-value:9.87E-4||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:SAY1(YGR263C)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:SIT4(YDL047W)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:TDA3(YHR009C_p)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:THO2(YNL139C)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids Gene:TPS2(YDR074W)|FD-Score:4.94|P-value:3.97E-7||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:TRP2(YER090W)|FD-Score:4.3|P-value:8.48E-6||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UME1(YPL139C)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p Gene:VAC14(YLR386W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VPS61(YDR136C_d)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YEL067C(YEL067C_p)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YGL218W(YGL218W_d)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance Gene:YHM2(YMR241W)|FD-Score:5.05|P-value:2.23E-7||SGD DESC:Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome Gene:YIL012W(YIL012W_d)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL086C(YIL086C_d)|FD-Score:3.13|P-value:8.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR366W(YLR366W_d)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A Gene:YML003W(YML003W_p)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Putative protein of unknown function Gene:YNR073C(YNR073C_p)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Putative mannitol dehydrogenase; identical in amino acid sequence to Dsf1p Gene:YOL073C(YOL073C_p)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; ortholog of fission yeast dsc2 Gene:YOR114W(YOR114W_p)|FD-Score:3.82|P-value:6.65E-5||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:YOR283W(YOR283W)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene Gene:YPR098C(YPR098C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YPT31(YER031C)|FD-Score:-3.42|P-value:3.09E-4||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:ZIP2(YGL249W)|FD-Score:-3.88|P-value:5.32E-5||SGD DESC:Meiosis-specific protein; involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR340C3.885.29E-50.84RPA43RNA polymerase I subunit A43
YLR026C3.846.15E-50.84SED5cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins
YCL041C_d3.000.001360.27YCL041C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W
YER094C2.730.003210.09PUP3Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
YNR017W2.640.004190.02TIM23Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel
YLR230W_d2.620.004460.06YLR230W_dDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity
YML025C2.560.005270.01YML6Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins
YPR082C2.550.005370.03DIB117-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein
YFL008W2.520.005870.05SMC1Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure
YGL075C2.470.006700.00MPS2Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p
YFL045C2.470.006750.00SEC53Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen
YNR011C2.470.006770.01PRP2RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16
YDR164C2.460.007030.01SEC1Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function
YER013W2.440.007260.01PRP22DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
YDR053W_d2.440.007430.02YDR053W_dPutative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR241W5.052.23E-7YHM2Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome
YDR074W4.943.97E-7TPS2Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress
YPR031W4.366.65E-6NTO1Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3
YER090W4.308.48E-6TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YPL139C4.261.04E-5UME1Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p
YNL123W4.141.77E-5NMA111Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases
YOR114W_p3.826.65E-5YOR114W_pPutative protein of unknown function; null mutant is viable
YGL078C3.768.38E-5DBP3RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype
YLR386W3.661.28E-4VAC14Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p
YPL015C3.601.60E-4HST2Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export
YPR098C3.532.06E-4YPR098CProtein of unknown function, localized to the mitochondrial outer membrane
YOR283W3.452.85E-4YOR283WPhosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene
YOL073C_p3.393.53E-4YOL073C_pSubunit of the DSC ubiquitin ligase complex; ortholog of fission yeast dsc2
YML003W_p3.324.43E-4YML003W_pPutative protein of unknown function
YLR366W_d3.304.80E-4YLR366W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A

GO enrichment analysis for SGTC_3258
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1461.73E-29SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0266667
0.1366.43E-26SGTC_14554262-0120 131.0 μMChemDiv (Drug-like library)15295870.144928plasma membrane duress
0.1316.34E-24SGTC_2409alverine citrate 64.0 μMMiscellaneous217180.0985916
0.1241.38E-21SGTC_22246650123 117.6 μMChembridge (Fragment library)29083700.0666667
0.1218.30E-21SGTC_15465-methoxyflavanone 78.7 μMTimTec (Pure natural product library)1477950.181818
0.1181.15E-19SGTC_1671st016596 74.5 μMTimTec (Natural product derivative library)28291670.111111
0.1163.16E-19SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.0833333
0.1163.79E-19SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.103448
0.1159.76E-19SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.111111
0.1132.30E-18SGTC_23047443016 91.4 μMChembridge (Fragment library)9398540.0705882
0.1094.78E-17SGTC_15412',3',6-trimethoxyflavone 64.0 μMTimTec (Pure natural product library)6888010.1875
0.1089.92E-17SGTC_11594092-0538 61.2 μMChemDiv (Drug-like library)7173010.0963855
0.1041.12E-15SGTC_22037227154 166.4 μMChembridge (Fragment library)8963040.12
0.1031.86E-15SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.047619TSC3-RPN4
0.1032.12E-15SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0449438

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3335914331529.63 μM0.6212124899691Chembridge (Drug-like library)365.471982.62615fatty acid desaturase (OLE1)
SGTC_3249913518349.47 μM0.5428574902779Chembridge (Drug-like library)365.471983.0271560S ribosome export
SGTC_3319913845016.76 μM0.4533332029690Chembridge (Drug-like library)379.498563.4915
SGTC_334491488623.9 μM0.4246584905352Chembridge (Drug-like library)349.816843.34914
SGTC_3342914779114.51 μM0.3684211838168Chembridge (Drug-like library)349.816843.34914
SGTC_3349914970323 μM0.364910494Chembridge (Drug-like library)351.44542.50915
SGTC_3257913920632.98 μM0.3150684910041Chembridge (Drug-like library)321.419423.04804
SGTC_334891494643.54 μM0.31251951442Chembridge (Drug-like library)379.498563.56915
SGTC_3340914663411.1 μM0.3086424907387Chembridge (Drug-like library)365.471983.14615
SGTC_3266913686049.47 μM0.3037974871271Chembridge (Drug-like library)343.424944.02214cell wall