k292-0812

3-butyl-2-[2-(3,4-dihydroxyphenyl)-2-oxoethyl]sulfanylthieno[3,2-d]pyrimidin-4-one

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_326
Screen concentration 17.5 μM
Source ChemDiv (Drug-like library)
PubChem CID 3579357
SMILES CCCCN1C(=O)C2=C(C=CS2)N=C1SCC(=O)C3=CC(=C(C=C3)O)O
Standardized SMILES CCCCN1C(=Nc2ccsc2C1=O)SCC(=O)c3ccc(O)c(O)c3
Molecular weight 390.4765
ALogP 4.29
H-bond donor count 2
H-bond acceptor count 7
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.35
% growth inhibition (Hom. pool) 2.26


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3579357
Download HIP data (tab-delimited text)  (excel)
Gene:BDP1(YNL039W)|FD-Score:-3.78|P-value:7.89E-5|Clearance:0||SGD DESC:Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs Gene:BRL1(YHR036W)|FD-Score:3.97|P-value:3.53E-5|Clearance:0.13||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:COF1(YLL050C)|FD-Score:3.15|P-value:8.21E-4|Clearance:0.27||SGD DESC:Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes Gene:MPE1(YKL059C)|FD-Score:6.62|P-value:1.77E-11|Clearance:2.61||SGD DESC:Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif Gene:NOP14(YDL148C)|FD-Score:-3.18|P-value:7.34E-4|Clearance:0||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:RER2(YBR002C)|FD-Score:-3.17|P-value:7.59E-4|Clearance:0||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPB8(YOR224C)|FD-Score:3.23|P-value:6.17E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPR2(YIR015W)|FD-Score:3.23|P-value:6.18E-4|Clearance:0.08||SGD DESC:Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress Gene:RSC58(YLR033W)|FD-Score:3.68|P-value:1.18E-4|Clearance:0.45||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SMC1(YFL008W)|FD-Score:-3.13|P-value:8.88E-4|Clearance:0||SGD DESC:Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure Gene:SWC4(YGR002C)|FD-Score:3.85|P-value:5.95E-5|Clearance:0.17||SGD DESC:Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex Gene:TRE2(YOR256C)|FD-Score:4.01|P-value:3.04E-5|Clearance:0.04||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:WBP1(YEL002C)|FD-Score:-3.47|P-value:2.58E-4|Clearance:0||SGD DESC:Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum Gene:YBL073W(YBL073W_d)|FD-Score:-3.32|P-value:4.47E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene AAR2/YBL074C Gene:BDP1(YNL039W)|FD-Score:-3.78|P-value:7.89E-5|Clearance:0||SGD DESC:Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs Gene:BRL1(YHR036W)|FD-Score:3.97|P-value:3.53E-5|Clearance:0.13||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:COF1(YLL050C)|FD-Score:3.15|P-value:8.21E-4|Clearance:0.27||SGD DESC:Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes Gene:MPE1(YKL059C)|FD-Score:6.62|P-value:1.77E-11|Clearance:2.61||SGD DESC:Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif Gene:NOP14(YDL148C)|FD-Score:-3.18|P-value:7.34E-4|Clearance:0||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:RER2(YBR002C)|FD-Score:-3.17|P-value:7.59E-4|Clearance:0||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPB8(YOR224C)|FD-Score:3.23|P-value:6.17E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPR2(YIR015W)|FD-Score:3.23|P-value:6.18E-4|Clearance:0.08||SGD DESC:Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress Gene:RSC58(YLR033W)|FD-Score:3.68|P-value:1.18E-4|Clearance:0.45||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SMC1(YFL008W)|FD-Score:-3.13|P-value:8.88E-4|Clearance:0||SGD DESC:Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure Gene:SWC4(YGR002C)|FD-Score:3.85|P-value:5.95E-5|Clearance:0.17||SGD DESC:Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex Gene:TRE2(YOR256C)|FD-Score:4.01|P-value:3.04E-5|Clearance:0.04||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:WBP1(YEL002C)|FD-Score:-3.47|P-value:2.58E-4|Clearance:0||SGD DESC:Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum Gene:YBL073W(YBL073W_d)|FD-Score:-3.32|P-value:4.47E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene AAR2/YBL074C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3579357
Download HOP data (tab-delimited text)  (excel)
Gene:ALT2(YDR111C_p)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Catalytically inactive paralog of ALT1, an alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p Gene:ARI1(YGL157W)|FD-Score:6.61|P-value:1.89E-11||SGD DESC:NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily Gene:AVL9(YLR114C)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:CAR2(YLR438W)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress Gene:CCE1(YKL011C)|FD-Score:4.43|P-value:4.72E-6||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:FRT2(YAL028W)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Tail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication Gene:GOR1(YNL274C)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Glyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:GTT2(YLL060C)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:HEL1(YKR017C_p)|FD-Score:3.92|P-value:4.36E-5||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU) Gene:HOS3(YPL116W)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Trichostatin A-insensitive homodimeric histone deacetylase (HDAC) with specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats Gene:HPM1(YIL110W)|FD-Score:-3.27|P-value:5.31E-4||SGD DESC:AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance Gene:LEA1(YPL213W)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein Gene:MET22(YOL064C)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MUS81(YDR386W)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:NNK1(YKL171W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm Gene:NOP13(YNL175C)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:PEF1(YGR058W)|FD-Score:3.77|P-value:8.12E-5||SGD DESC:Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly Gene:PFK1(YGR240C)|FD-Score:-3.45|P-value:2.86E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PMT6(YGR199W)|FD-Score:4.75|P-value:1.02E-6||SGD DESC:Protein O-mannosyltransferase, transfers mannose from dolichyl phosphate-D-mannose to protein serine/threonine residues of secretory proteins; reaction is essential for cell wall rigidity; member of a family of mannosyltransferases Gene:QDR3(YBR043C)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin Gene:SKG3(YLR187W_p)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication Gene:TRP4(YDR354W)|FD-Score:-3.25|P-value:5.74E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBS1(YBR165W)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity Gene:URN1(YPR152C)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Putative protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase Gene:YBR051W(YBR051W_d)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partiallly overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase Gene:YDR056C(YDR056C_p)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein Gene:YDR338C(YDR338C_p)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Putative protein of unknown function, member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily Gene:YLR225C(YLR225C_p)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene Gene:YML002W(YML002W_p)|FD-Score:3.85|P-value:5.87E-5||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YNL120C(YNL120C_d)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene Gene:YOR050C(YOR050C_d)|FD-Score:-3.12|P-value:9.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutation is viable Gene:YPC1(YBR183W)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Alkaline ceramidase; also has reverse (CoA-independent) ceramide synthase activity; catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance; YPC1 has a paralog, YDC1, that arose from the whole genome duplication Gene:YPR077C(YPR077C_d)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:ALT2(YDR111C_p)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Catalytically inactive paralog of ALT1, an alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p Gene:ARI1(YGL157W)|FD-Score:6.61|P-value:1.89E-11||SGD DESC:NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily Gene:AVL9(YLR114C)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:CAR2(YLR438W)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress Gene:CCE1(YKL011C)|FD-Score:4.43|P-value:4.72E-6||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:FRT2(YAL028W)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Tail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication Gene:GOR1(YNL274C)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Glyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:GTT2(YLL060C)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:HEL1(YKR017C_p)|FD-Score:3.92|P-value:4.36E-5||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU) Gene:HOS3(YPL116W)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Trichostatin A-insensitive homodimeric histone deacetylase (HDAC) with specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats Gene:HPM1(YIL110W)|FD-Score:-3.27|P-value:5.31E-4||SGD DESC:AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance Gene:LEA1(YPL213W)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein Gene:MET22(YOL064C)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MUS81(YDR386W)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:NNK1(YKL171W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm Gene:NOP13(YNL175C)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:PEF1(YGR058W)|FD-Score:3.77|P-value:8.12E-5||SGD DESC:Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly Gene:PFK1(YGR240C)|FD-Score:-3.45|P-value:2.86E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PMT6(YGR199W)|FD-Score:4.75|P-value:1.02E-6||SGD DESC:Protein O-mannosyltransferase, transfers mannose from dolichyl phosphate-D-mannose to protein serine/threonine residues of secretory proteins; reaction is essential for cell wall rigidity; member of a family of mannosyltransferases Gene:QDR3(YBR043C)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin Gene:SKG3(YLR187W_p)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication Gene:TRP4(YDR354W)|FD-Score:-3.25|P-value:5.74E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBS1(YBR165W)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity Gene:URN1(YPR152C)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Putative protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase Gene:YBR051W(YBR051W_d)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partiallly overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase Gene:YDR056C(YDR056C_p)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein Gene:YDR338C(YDR338C_p)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Putative protein of unknown function, member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily Gene:YLR225C(YLR225C_p)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene Gene:YML002W(YML002W_p)|FD-Score:3.85|P-value:5.87E-5||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YNL120C(YNL120C_d)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene Gene:YOR050C(YOR050C_d)|FD-Score:-3.12|P-value:9.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutation is viable Gene:YPC1(YBR183W)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Alkaline ceramidase; also has reverse (CoA-independent) ceramide synthase activity; catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance; YPC1 has a paralog, YDC1, that arose from the whole genome duplication Gene:YPR077C(YPR077C_d)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL059C6.621.77E-112.61MPE1Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif
YOR256C4.013.04E-50.04TRE2Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication
YHR036W3.973.53E-50.13BRL1Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
YGR002C3.855.95E-50.17SWC4Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex
YLR033W3.681.18E-40.45RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YOR224C3.236.17E-44.05E-4RPB8RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III
YIR015W3.236.18E-40.08RPR2Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress
YLL050C3.158.21E-40.27COF1Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes
YKR083C2.870.002020.08DAD2Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YNL181W_p2.800.002570.01YNL181W_pPutative oxidoreductase; required for cell viability
YDL060W2.790.002610.02TSR1Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
YDR087C2.770.002800.04RRP1Essential evolutionarily conserved nucleolar protein necessary for biogenesis of 60S ribosomal subunits and processing of pre-rRNAs to mature rRNAs, associated with several distinct 66S pre-ribosomal particles
YHR088W2.730.003170.01RPF1Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA
YNL290W2.720.003280.00RFC3Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YPL190C2.720.003300.04NAB3RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL157W6.611.89E-11ARI1NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily
YGR199W4.751.02E-6PMT6Protein O-mannosyltransferase, transfers mannose from dolichyl phosphate-D-mannose to protein serine/threonine residues of secretory proteins; reaction is essential for cell wall rigidity; member of a family of mannosyltransferases
YKL011C4.434.72E-6CCE1Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA
YNL120C_d4.221.20E-5YNL120C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene
YPL213W4.151.65E-5LEA1Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein
YLL060C4.102.03E-5GTT2Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress
YKR017C_p3.924.36E-5HEL1_pRING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU)
YML002W_p3.855.87E-5YML002W_pPutative protein of unknown function; expression induced by heat and by calcium shortage
YGR058W3.778.12E-5PEF1Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
YKL171W3.711.02E-4NNK1Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm
YLR187W_p3.571.75E-4SKG3_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication
YLR225C_p3.393.50E-4YLR225C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene
YDR338C_p3.393.53E-4YDR338C_pPutative protein of unknown function, member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily
YBR165W3.373.72E-4UBS1Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity
YNL274C3.304.80E-4GOR1Glyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_326
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0823.33E-10SGTC_22297354935 190.0 μMChembridge (Fragment library)3181120.103896RSC complex & mRNA processing
0.0791.52E-9SGTC_3252810-4341 181.0 μMChemDiv (Drug-like library)7749700.0952381amide catabolism
0.0732.00E-8SGTC_14464239-0190 62.0 μMChemDiv (Drug-like library)13400850.117021
0.0714.64E-8SGTC_9701315-0089 19.5 μMChemDiv (Drug-like library)68110830.106383RSC & ERG11
0.0715.29E-8SGTC_328k915-0120 63.5 μMChemDiv (Drug-like library)66245430.0879121TSC3-RPN4
0.0706.40E-8SGTC_22167189485 200.0 μMChembridge (Fragment library)7312730.155844
0.0681.99E-7SGTC_21035378138 10.0 μMChembridge (Fragment library)2441420.0810811
0.0604.38E-6SGTC_23296138490 200.0 μMChembridge (Fragment library)7200190.12987
0.0595.62E-6SGTC_3241315-0088 19.6 μMChemDiv (Drug-like library)67874210.0898876RSC & ERG11
0.0588.13E-6SGTC_21866124449 200.0 μMChembridge (Fragment library)38341820.107143RSC complex & mRNA processing
0.0589.61E-6SGTC_3234013-0906 34.3 μMChemDiv (Drug-like library)29100860.0707071ERG2
0.0552.23E-5SGTC_5720960-0087 17.7 μMChemDiv (Drug-like library)67963650.108911
0.0552.49E-5SGTC_643k048-0106 28.4 μMChemDiv (Drug-like library)67639200.0804598RSC & ERG11
0.0542.88E-5SGTC_1652st011932 78.0 μMTimTec (Natural product derivative library)15510760.117647RSC complex & mRNA processing
0.0543.85E-5SGTC_1567st069303 68.4 μMTimTec (Pure natural product library)55813190.0618557mitochondrial processes

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_711k292-0785179 μM0.3636364146315ChemDiv (Drug-like library)240.345083.42413DNA intercalators
SGTC_3084911687749.47 μM0.30769227241325Chembridge (Drug-like library)385.28814.07205
SGTC_1000086-012849.59 μM0.25714393297ChemDiv (Drug-like library)248.234682.08504copper-dependent oxidative stress
SGTC_534k292-078863.4 μM0.2465754146317ChemDiv (Drug-like library)254.371663.67613mitochondrial processes
SGTC_12760871-006894.2 μM0.2461543414906ChemDiv (Drug-like library)261.319562.57104
SGTC_2680butyl paraben22.24 μM0.2424247184Microsource (Natural product library)194.227062.77213amide catabolism
SGTC_6660272-0154136 μM0.2307696870312ChemDiv (Drug-like library)276.357342.41523cell wall
SGTC_9983996-0085177 μM0.228916715391ChemDiv (Drug-like library)340.399543.15316
SGTC_790052-000794.09 μM0.2285712801472ChemDiv (Drug-like library)276.370664.63813
SGTC_1038k292-0204154 μM0.2266674454212ChemDiv (Drug-like library)288.38788413