9137442

5-amino-N-cyclohexyl-1-(2-methylphenyl)triazole-4-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3264
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 17015726
SMILES CC1=CC=CC=C1N2C(=C(N=N2)C(=O)NC3CCCCC3)N
Standardized SMILES Cc1ccccc1n2nnc(C(=O)NC3CCCCC3)c2N
Molecular weight 299.3708
ALogP 2.95
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.45
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17015726
Download HIP data (tab-delimited text)  (excel)
Gene:CDC24(YAL041W)|FD-Score:-3.75|P-value:8.90E-5|Clearance:0||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:CDC45(YLR103C)|FD-Score:-3.71|P-value:1.03E-4|Clearance:0||SGD DESC:DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes Gene:COG2(YGR120C)|FD-Score:4.08|P-value:2.26E-5|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAD1(YDR016C)|FD-Score:-4.38|P-value:5.87E-6|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DUT1(YBR252W)|FD-Score:-4.32|P-value:7.90E-6|Clearance:0||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:MRS6(YOR370C)|FD-Score:-3.12|P-value:9.09E-4|Clearance:0||SGD DESC:Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress Gene:MSL5(YLR116W)|FD-Score:3.1|P-value:9.59E-4|Clearance:0.01||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:MTR3(YGR158C)|FD-Score:-3.1|P-value:9.76E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NFS1(YCL017C)|FD-Score:3.6|P-value:1.62E-4|Clearance:0.1||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:POP6(YGR030C)|FD-Score:4.07|P-value:2.30E-5|Clearance:0.03||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RAD53(YPL153C)|FD-Score:-3.95|P-value:3.98E-5|Clearance:0||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RET1(YOR207C)|FD-Score:6.53|P-value:3.19E-11|Clearance:1.44||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPP1(YHR062C)|FD-Score:5.52|P-value:1.66E-8|Clearance:1.44||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS31(YLR167W)|FD-Score:3.43|P-value:3.03E-4|Clearance:0.33||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:RRP17(YDR412W)|FD-Score:3.1|P-value:9.77E-4|Clearance:0.13||SGD DESC:Component of the pre-60S pre-ribosomal particle; required for cell viability under standard (aerobic) conditions but not under anaerobic conditions; exonuclease required for 5′ end processing of pre-60S ribosomal RNA Gene:SEC23(YPR181C)|FD-Score:5.75|P-value:4.43E-9|Clearance:1.44||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SMC4(YLR086W)|FD-Score:4.05|P-value:2.57E-5|Clearance:0.45||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:SRP102(YKL154W)|FD-Score:-3.73|P-value:9.64E-5|Clearance:0||SGD DESC:Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane Gene:UTP14(YML093W)|FD-Score:3.49|P-value:2.41E-4|Clearance:0.06||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YDL196W(YDL196W_d)|FD-Score:-3.32|P-value:4.56E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:CDC24(YAL041W)|FD-Score:-3.75|P-value:8.90E-5|Clearance:0||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:CDC45(YLR103C)|FD-Score:-3.71|P-value:1.03E-4|Clearance:0||SGD DESC:DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes Gene:COG2(YGR120C)|FD-Score:4.08|P-value:2.26E-5|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAD1(YDR016C)|FD-Score:-4.38|P-value:5.87E-6|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DUT1(YBR252W)|FD-Score:-4.32|P-value:7.90E-6|Clearance:0||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:MRS6(YOR370C)|FD-Score:-3.12|P-value:9.09E-4|Clearance:0||SGD DESC:Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress Gene:MSL5(YLR116W)|FD-Score:3.1|P-value:9.59E-4|Clearance:0.01||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:MTR3(YGR158C)|FD-Score:-3.1|P-value:9.76E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NFS1(YCL017C)|FD-Score:3.6|P-value:1.62E-4|Clearance:0.1||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:POP6(YGR030C)|FD-Score:4.07|P-value:2.30E-5|Clearance:0.03||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RAD53(YPL153C)|FD-Score:-3.95|P-value:3.98E-5|Clearance:0||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RET1(YOR207C)|FD-Score:6.53|P-value:3.19E-11|Clearance:1.44||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPP1(YHR062C)|FD-Score:5.52|P-value:1.66E-8|Clearance:1.44||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS31(YLR167W)|FD-Score:3.43|P-value:3.03E-4|Clearance:0.33||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:RRP17(YDR412W)|FD-Score:3.1|P-value:9.77E-4|Clearance:0.13||SGD DESC:Component of the pre-60S pre-ribosomal particle; required for cell viability under standard (aerobic) conditions but not under anaerobic conditions; exonuclease required for 5′ end processing of pre-60S ribosomal RNA Gene:SEC23(YPR181C)|FD-Score:5.75|P-value:4.43E-9|Clearance:1.44||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SMC4(YLR086W)|FD-Score:4.05|P-value:2.57E-5|Clearance:0.45||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:SRP102(YKL154W)|FD-Score:-3.73|P-value:9.64E-5|Clearance:0||SGD DESC:Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane Gene:UTP14(YML093W)|FD-Score:3.49|P-value:2.41E-4|Clearance:0.06||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YDL196W(YDL196W_d)|FD-Score:-3.32|P-value:4.56E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17015726
Download HOP data (tab-delimited text)  (excel)
Gene:AEP3(YPL005W)|FD-Score:-3.33|P-value:4.36E-4||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:ARC18(YLR370C)|FD-Score:-4.59|P-value:2.22E-6||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:AST1(YBL069W)|FD-Score:4.94|P-value:3.83E-7||SGD DESC:Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication Gene:ATG12(YBR217W)|FD-Score:-3.15|P-value:8.10E-4||SGD DESC:Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation Gene:BNA2(YJR078W)|FD-Score:3.22|P-value:6.30E-4||SGD DESC:Putative tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase, required for de novo biosynthesis of NAD from tryptophan via kynurenine; interacts genetically with telomere capping gene CDC13; regulated by Hst1p and Aftp Gene:BUD4(YJR092W)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Anillin-like protein involved in bud-site selection; required for the axial budding pattern; localizes with septins to the bud neck in mitosis and may constitute an axial landmark for the next round of budding; required for the formation and disassembly of the double septin ring structure, and generally for septin organization; in vivo substrate of Cdc28p/Clb2p Gene:COA1(YIL157C)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly Gene:COX9(YDL067C)|FD-Score:4.13|P-value:1.78E-5||SGD DESC:Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:DNF1(YER166W)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:DPB3(YBR278W)|FD-Score:-3.28|P-value:5.11E-4||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:DUF1(YOL087C)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:EMC5(YIL027C)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:GAL80(YML051W)|FD-Score:-3.74|P-value:9.12E-5||SGD DESC:Transcriptional regulator involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding Gene:HXT17(YNR072W)|FD-Score:3.78|P-value:7.71E-5||SGD DESC:Hexose transporter, up-regulated in media containing raffinose and galactose at pH 7.7 versus pH 4.7, repressed by high levels of glucose Gene:IRC13(YOR235W_d)|FD-Score:5.64|P-value:8.66E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:IZH1(YDR492W)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc Gene:MAL11(YGR289C)|FD-Score:3.93|P-value:4.19E-5||SGD DESC:Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization Gene:MIR1(YJR077C)|FD-Score:4.07|P-value:2.40E-5||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated Gene:NDL1(YLR254C)|FD-Score:3.87|P-value:5.43E-5||SGD DESC:Homolog of nuclear distribution factor NudE, NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends Gene:NEW1(YPL226W)|FD-Score:-3.12|P-value:8.90E-4||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:NOP6(YDL213C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes Gene:OCT1(YKL134C)|FD-Score:-3.11|P-value:9.25E-4||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:PET18(YCR020C)|FD-Score:5.21|P-value:9.60E-8||SGD DESC:Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin Gene:PEX1(YKL197C)|FD-Score:-5.79|P-value:3.58E-9||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis Gene:PHO84(YML123C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PKR1(YMR123W)|FD-Score:-3.37|P-value:3.77E-4||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PXA1(YPL147W)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter ABCD1 and ABCD2 and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder Gene:RBK1(YCR036W)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Putative ribokinase Gene:RGI1(YER067W)|FD-Score:-3.87|P-value:5.45E-5||SGD DESC:Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:ROM2(YLR371W)|FD-Score:-3.89|P-value:5.07E-5||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL24B(YGR148C)|FD-Score:-3.21|P-value:6.71E-4||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RSM18(YER050C)|FD-Score:-3.23|P-value:6.14E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:RTC6(YPL183W-A)|FD-Score:-4.01|P-value:3.04E-5||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SCD6(YPR129W)|FD-Score:-3.37|P-value:3.75E-4||SGD DESC:Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress Gene:SLG1(YOR008C)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SPE3(YPR069C)|FD-Score:7.92|P-value:1.17E-15||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SPO1(YNL012W)|FD-Score:4.17|P-value:1.53E-5||SGD DESC:Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B Gene:STE50(YCL032W)|FD-Score:-3.3|P-value:4.84E-4||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:STR3(YGL184C)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Peroxisomal cystathionine beta-lyase, converts cystathionine into homocysteine; may be redox regulated by Gto1p Gene:SUR4(YLR372W)|FD-Score:4.61|P-value:1.97E-6||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TDA5(YLR426W_p)|FD-Score:-3.26|P-value:5.64E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TOS3(YGL179C)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Protein kinase, related to and functionally redundant with Elm1p and Sak1p for the phosphorylation and activation of Snf1p; functionally orthologous to LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:UFD2(YDL190C)|FD-Score:4.49|P-value:3.58E-6||SGD DESC:Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 Gene:URA1(YKL216W)|FD-Score:-4.36|P-value:6.59E-6||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:YER119C-A(YER119C-A_d)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YFL041W-A(YFL041W-A_p)|FD-Score:-3.29|P-value:4.96E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGR064W(YGR064W_d)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YGR069W(YGR069W_d)|FD-Score:3.75|P-value:8.84E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHR035W(YHR035W_p)|FD-Score:4.58|P-value:2.29E-6||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YIL024C(YIL024C_p)|FD-Score:-3.19|P-value:7.23E-4||SGD DESC:Putative protein of unknown function; non-essential gene; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p Gene:YLR152C(YLR152C_p)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Putative protein of unknown function; YLR152C is not an essential gene Gene:YLR225C(YLR225C_p)|FD-Score:-3.22|P-value:6.50E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene Gene:YLR257W(YLR257W_p)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Protein of unknown function; protein abundance increases in response to DNA replication stress Gene:YMR172C-A(YMR172C-A_d)|FD-Score:-3.28|P-value:5.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR279C(YMR279C)|FD-Score:-4.31|P-value:8.16E-6||SGD DESC:Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication Gene:YNL184C(YNL184C_p)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR111W(YOR111W_p)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Putative protein of unknown function Gene:YOR121C(YOR121C_d)|FD-Score:-3.13|P-value:8.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YOR376W-A(YOR376W-A_p)|FD-Score:3.15|P-value:8.28E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPL039W(YPL039W_p)|FD-Score:5.37|P-value:4.01E-8||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:YPL185W(YPL185W_d)|FD-Score:-3.27|P-value:5.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C Gene:YPR059C(YPR059C_d)|FD-Score:4.86|P-value:5.76E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W Gene:YPR063C(YPR063C_p)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:ER-localized protein of unknown function Gene:YPT31(YER031C)|FD-Score:-3.37|P-value:3.74E-4||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:AEP3(YPL005W)|FD-Score:-3.33|P-value:4.36E-4||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:ARC18(YLR370C)|FD-Score:-4.59|P-value:2.22E-6||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:AST1(YBL069W)|FD-Score:4.94|P-value:3.83E-7||SGD DESC:Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication Gene:ATG12(YBR217W)|FD-Score:-3.15|P-value:8.10E-4||SGD DESC:Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation Gene:BNA2(YJR078W)|FD-Score:3.22|P-value:6.30E-4||SGD DESC:Putative tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase, required for de novo biosynthesis of NAD from tryptophan via kynurenine; interacts genetically with telomere capping gene CDC13; regulated by Hst1p and Aftp Gene:BUD4(YJR092W)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Anillin-like protein involved in bud-site selection; required for the axial budding pattern; localizes with septins to the bud neck in mitosis and may constitute an axial landmark for the next round of budding; required for the formation and disassembly of the double septin ring structure, and generally for septin organization; in vivo substrate of Cdc28p/Clb2p Gene:COA1(YIL157C)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly Gene:COX9(YDL067C)|FD-Score:4.13|P-value:1.78E-5||SGD DESC:Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:DNF1(YER166W)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:DPB3(YBR278W)|FD-Score:-3.28|P-value:5.11E-4||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:DUF1(YOL087C)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:EMC5(YIL027C)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:GAL80(YML051W)|FD-Score:-3.74|P-value:9.12E-5||SGD DESC:Transcriptional regulator involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding Gene:HXT17(YNR072W)|FD-Score:3.78|P-value:7.71E-5||SGD DESC:Hexose transporter, up-regulated in media containing raffinose and galactose at pH 7.7 versus pH 4.7, repressed by high levels of glucose Gene:IRC13(YOR235W_d)|FD-Score:5.64|P-value:8.66E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:IZH1(YDR492W)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc Gene:MAL11(YGR289C)|FD-Score:3.93|P-value:4.19E-5||SGD DESC:Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization Gene:MIR1(YJR077C)|FD-Score:4.07|P-value:2.40E-5||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated Gene:NDL1(YLR254C)|FD-Score:3.87|P-value:5.43E-5||SGD DESC:Homolog of nuclear distribution factor NudE, NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends Gene:NEW1(YPL226W)|FD-Score:-3.12|P-value:8.90E-4||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:NOP6(YDL213C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes Gene:OCT1(YKL134C)|FD-Score:-3.11|P-value:9.25E-4||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:PET18(YCR020C)|FD-Score:5.21|P-value:9.60E-8||SGD DESC:Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin Gene:PEX1(YKL197C)|FD-Score:-5.79|P-value:3.58E-9||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis Gene:PHO84(YML123C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PKR1(YMR123W)|FD-Score:-3.37|P-value:3.77E-4||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PXA1(YPL147W)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter ABCD1 and ABCD2 and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder Gene:RBK1(YCR036W)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Putative ribokinase Gene:RGI1(YER067W)|FD-Score:-3.87|P-value:5.45E-5||SGD DESC:Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:ROM2(YLR371W)|FD-Score:-3.89|P-value:5.07E-5||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL24B(YGR148C)|FD-Score:-3.21|P-value:6.71E-4||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RSM18(YER050C)|FD-Score:-3.23|P-value:6.14E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:RTC6(YPL183W-A)|FD-Score:-4.01|P-value:3.04E-5||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SCD6(YPR129W)|FD-Score:-3.37|P-value:3.75E-4||SGD DESC:Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress Gene:SLG1(YOR008C)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SPE3(YPR069C)|FD-Score:7.92|P-value:1.17E-15||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SPO1(YNL012W)|FD-Score:4.17|P-value:1.53E-5||SGD DESC:Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B Gene:STE50(YCL032W)|FD-Score:-3.3|P-value:4.84E-4||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:STR3(YGL184C)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Peroxisomal cystathionine beta-lyase, converts cystathionine into homocysteine; may be redox regulated by Gto1p Gene:SUR4(YLR372W)|FD-Score:4.61|P-value:1.97E-6||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TDA5(YLR426W_p)|FD-Score:-3.26|P-value:5.64E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TOS3(YGL179C)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Protein kinase, related to and functionally redundant with Elm1p and Sak1p for the phosphorylation and activation of Snf1p; functionally orthologous to LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:UFD2(YDL190C)|FD-Score:4.49|P-value:3.58E-6||SGD DESC:Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 Gene:URA1(YKL216W)|FD-Score:-4.36|P-value:6.59E-6||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:YER119C-A(YER119C-A_d)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YFL041W-A(YFL041W-A_p)|FD-Score:-3.29|P-value:4.96E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGR064W(YGR064W_d)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YGR069W(YGR069W_d)|FD-Score:3.75|P-value:8.84E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHR035W(YHR035W_p)|FD-Score:4.58|P-value:2.29E-6||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YIL024C(YIL024C_p)|FD-Score:-3.19|P-value:7.23E-4||SGD DESC:Putative protein of unknown function; non-essential gene; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p Gene:YLR152C(YLR152C_p)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Putative protein of unknown function; YLR152C is not an essential gene Gene:YLR225C(YLR225C_p)|FD-Score:-3.22|P-value:6.50E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene Gene:YLR257W(YLR257W_p)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Protein of unknown function; protein abundance increases in response to DNA replication stress Gene:YMR172C-A(YMR172C-A_d)|FD-Score:-3.28|P-value:5.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR279C(YMR279C)|FD-Score:-4.31|P-value:8.16E-6||SGD DESC:Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication Gene:YNL184C(YNL184C_p)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR111W(YOR111W_p)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Putative protein of unknown function Gene:YOR121C(YOR121C_d)|FD-Score:-3.13|P-value:8.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YOR376W-A(YOR376W-A_p)|FD-Score:3.15|P-value:8.28E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPL039W(YPL039W_p)|FD-Score:5.37|P-value:4.01E-8||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:YPL185W(YPL185W_d)|FD-Score:-3.27|P-value:5.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C Gene:YPR059C(YPR059C_d)|FD-Score:4.86|P-value:5.76E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W Gene:YPR063C(YPR063C_p)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:ER-localized protein of unknown function Gene:YPT31(YER031C)|FD-Score:-3.37|P-value:3.74E-4||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR207C6.533.19E-111.44RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YPR181C5.754.43E-91.44SEC23GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p
YHR062C5.521.66E-81.44RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YGR120C4.082.26E-50.00COG2Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YGR030C4.072.30E-50.03POP6Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YLR086W4.052.57E-50.45SMC4Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate
YCL017C3.601.62E-40.10NFS1Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria
YML093W3.492.41E-40.06UTP14Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit
YLR167W3.433.03E-40.33RPS31Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog
YLR116W3.109.59E-40.01MSL5Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA
YDR412W3.109.77E-40.13RRP17Component of the pre-60S pre-ribosomal particle; required for cell viability under standard (aerobic) conditions but not under anaerobic conditions; exonuclease required for 5′ end processing of pre-60S ribosomal RNA
YDL141W2.970.001510.03BPL1Biotin:apoprotein ligase, covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation
YDL152W_d2.940.001650.00YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YER127W2.940.001670.02LCP5Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus
YDR361C2.910.001780.01BCP1Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPR069C7.921.17E-15SPE3Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells
YOR235W_d5.648.66E-9IRC13_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci
YPL039W_p5.374.01E-8YPL039W_pPutative protein of unknown function; YPL039W is not an essential gene
YCR020C5.219.60E-8PET18Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin
YBL069W4.943.83E-7AST1Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication
YPR059C_d4.865.76E-7YPR059C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W
YLR372W4.611.97E-6SUR4Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
YHR035W_p4.582.29E-6YHR035W_pPutative protein of unknown function; not an essential gene
YDL190C4.493.58E-6UFD2Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3
YOL087C4.201.35E-5DUF1Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid
YNL012W4.171.53E-5SPO1Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B
YDL067C4.131.78E-5COX9Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain
YJR077C4.072.40E-5MIR1Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated
YGR289C3.934.19E-5MAL11Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization
YLR254C3.875.43E-5NDL1Homolog of nuclear distribution factor NudE, NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends

GO enrichment analysis for SGTC_3264
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1072.06E-16SGTC_32619137475 49.5 μMChembridge (Drug-like library)170276900.126582RPP1 & pyrimidine depletion
0.0943.82E-13SGTC_1643st009866 20.2 μMTimTec (Natural product derivative library)31562730.1375
0.0936.36E-13SGTC_32719137798 49.5 μMChembridge (Drug-like library)64983260.666667
0.0931.02E-12SGTC_23067482397 179.5 μMChembridge (Fragment library)8914480.0694444RPP1 & pyrimidine depletion
0.0931.05E-12SGTC_23236148992 148.3 μMChembridge (Fragment library)28882200.142857
0.0895.78E-12SGTC_31609100216 49.5 μMChembridge (Drug-like library)171910390.148148
0.0872.41E-11SGTC_503tyrphostin 8 294.0 μMICCB bioactive library929700.0645161TSC3-RPN4
0.0855.15E-11SGTC_8201582-0060 59.9 μMChemDiv (Drug-like library)38488400.109589
0.0841.31E-10SGTC_1661st013063 9.7 μMTimTec (Natural product derivative library)7213950.106667
0.0764.38E-9SGTC_24984'-Methoxychalcone 54.6 μMMicrosource (Natural product library)6418180.138462RPP1 & pyrimidine depletion
0.0757.45E-9SGTC_29217974240 10.7 μMChembridge (Drug-like library)29790740.106667
0.0732.25E-8SGTC_32249130643 49.5 μMChembridge (Drug-like library)176443950.08RPP1 & pyrimidine depletion
0.0714.69E-8SGTC_265mycophenolic acid 32.9 μMMiscellaneous4465410.0823529RPP1 & pyrimidine depletion
0.0715.48E-8SGTC_2522biochanin a 4.9 μMMicrosource (Natural product library)52803730.0649351RNA pol III & RNase P/MRP
0.0706.01E-8SGTC_16014'-hydroxyflavanone 55.5 μMTimTec (Pure natural product library)1655060.117647RPP1 & pyrimidine depletion

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3271913779849.47 μM0.6666676498326Chembridge (Drug-like library)299.370842.94824
SGTC_3270913756349.47 μM0.62745117015724Chembridge (Drug-like library)293.32322.66624RPP1 & pyrimidine depletion
SGTC_3212912830749.47 μM0.58928627243542Chembridge (Drug-like library)350.213742.75424
SGTC_3355915105844.05 μM0.58181817015728Chembridge (Drug-like library)311.3136632.87125RNA processing & uracil transport
SGTC_3274913829849.47 μM0.48214317015756Chembridge (Drug-like library)293.32322.66624
SGTC_3228913179949.47 μM0.44262317015818Chembridge (Drug-like library)307.349783.15224amide catabolism
SGTC_3225913081949.47 μM0.4098364547121Chembridge (Drug-like library)293.32322.66624amide catabolism
SGTC_3245913482149.47 μM0.38709717014901Chembridge (Drug-like library)307.349783.12224
SGTC_3201911399949.47 μM0.38095216649224Chembridge (Drug-like library)336.38773.90314
SGTC_2754granisetron64.02 μM0.3283585284566Miscellaneous312.409362.40113