9137266

N-(3,4-dichlorophenyl)-1-(2,2-dimethylpropanoyl)piperidine-4-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3265
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 17224265
SMILES CC(C)(C)C(=O)N1CCC(CC1)C(=O)NC2=CC(=C(C=C2)Cl)Cl
Standardized SMILES CC(C)(C)C(=O)N1CCC(CC1)C(=O)Nc2ccc(Cl)c(Cl)c2
Molecular weight 357.2748
ALogP 3.71
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.45
% growth inhibition (Hom. pool) 1.11


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17224265
Download HIP data (tab-delimited text)  (excel)
Gene:CDC123(YLR215C)|FD-Score:-3.26|P-value:5.48E-4|Clearance:0||SGD DESC:Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein Gene:DPM1(YPR183W)|FD-Score:3.31|P-value:4.68E-4|Clearance:0.18||SGD DESC:Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation Gene:DUT1(YBR252W)|FD-Score:6.15|P-value:3.92E-10|Clearance:1.12||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:FRS2(YFL022C)|FD-Score:-3.3|P-value:4.92E-4|Clearance:0||SGD DESC:Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar Gene:GLC7(YER133W)|FD-Score:3.12|P-value:8.89E-4|Clearance:0.15||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:NAF1(YNL124W)|FD-Score:3.98|P-value:3.51E-5|Clearance:0.67||SGD DESC:RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p Gene:PFY1(YOR122C)|FD-Score:-3.79|P-value:7.42E-5|Clearance:0||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:RCL1(YOL010W)|FD-Score:-3.77|P-value:8.21E-5|Clearance:0||SGD DESC:Endonuclease that cleaves pre-rRNA at site A2 for 18S rRNA biogenesis; subunit of U3-containing 90S preribosome processome complex involved in small ribosomal subunit assembly; stimulates Bms1p GTPase and U3 binding activity; similar to RNA cyclase-like proteins but no cyclase activity detected Gene:RHO3(YIL118W)|FD-Score:-3.28|P-value:5.16E-4|Clearance:0||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p Gene:YGR265W(YGR265W_d)|FD-Score:5.03|P-value:2.49E-7|Clearance:1.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:YHC1(YLR298C)|FD-Score:-3.98|P-value:3.51E-5|Clearance:0||SGD DESC:Component of the U1 snRNP complex required for pre-mRNA splicing; putative ortholog of human U1C protein, which is involved in formation of a complex between U1 snRNP and the pre-mRNA 5' splice site Gene:CDC123(YLR215C)|FD-Score:-3.26|P-value:5.48E-4|Clearance:0||SGD DESC:Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein Gene:DPM1(YPR183W)|FD-Score:3.31|P-value:4.68E-4|Clearance:0.18||SGD DESC:Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation Gene:DUT1(YBR252W)|FD-Score:6.15|P-value:3.92E-10|Clearance:1.12||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:FRS2(YFL022C)|FD-Score:-3.3|P-value:4.92E-4|Clearance:0||SGD DESC:Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar Gene:GLC7(YER133W)|FD-Score:3.12|P-value:8.89E-4|Clearance:0.15||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:NAF1(YNL124W)|FD-Score:3.98|P-value:3.51E-5|Clearance:0.67||SGD DESC:RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p Gene:PFY1(YOR122C)|FD-Score:-3.79|P-value:7.42E-5|Clearance:0||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:RCL1(YOL010W)|FD-Score:-3.77|P-value:8.21E-5|Clearance:0||SGD DESC:Endonuclease that cleaves pre-rRNA at site A2 for 18S rRNA biogenesis; subunit of U3-containing 90S preribosome processome complex involved in small ribosomal subunit assembly; stimulates Bms1p GTPase and U3 binding activity; similar to RNA cyclase-like proteins but no cyclase activity detected Gene:RHO3(YIL118W)|FD-Score:-3.28|P-value:5.16E-4|Clearance:0||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p Gene:YGR265W(YGR265W_d)|FD-Score:5.03|P-value:2.49E-7|Clearance:1.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:YHC1(YLR298C)|FD-Score:-3.98|P-value:3.51E-5|Clearance:0||SGD DESC:Component of the U1 snRNP complex required for pre-mRNA splicing; putative ortholog of human U1C protein, which is involved in formation of a complex between U1 snRNP and the pre-mRNA 5' splice site

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17224265
Download HOP data (tab-delimited text)  (excel)
Gene:ACS1(YAL054C)|FD-Score:3.86|P-value:5.76E-5||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions Gene:AHC1(YOR023C)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex Gene:ALG5(YPL227C)|FD-Score:-5.6|P-value:1.08E-8||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:APP1(YNL094W)|FD-Score:3.84|P-value:6.20E-5||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:ATP14(YLR295C)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:BDH2(YAL061W_p)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3 Gene:COG6(YNL041C)|FD-Score:-3.74|P-value:9.16E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DIG1(YPL049C)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:ELP2(YGR200C)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:FRE5(YOR384W)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ILM1(YJR118C)|FD-Score:4.29|P-value:9.00E-6||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:IOC3(YFR013W)|FD-Score:-3.1|P-value:9.67E-4||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:LTE1(YAL024C)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MAC1(YMR021C)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MNN2(YBR015C)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRS3(YJL133W)|FD-Score:4.66|P-value:1.61E-6||SGD DESC:Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication Gene:MSB4(YOL112W)|FD-Score:-3.29|P-value:4.98E-4||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:PAU7(YAR020C)|FD-Score:-3.15|P-value:8.25E-4||SGD DESC:Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme Gene:PIB1(YDR313C)|FD-Score:3.81|P-value:7.02E-5||SGD DESC:RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain Gene:PTC6(YCR079W)|FD-Score:-4.44|P-value:4.54E-6||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase Gene:RGT1(YKL038W)|FD-Score:-5.76|P-value:4.30E-9||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:ROM2(YLR371W)|FD-Score:-3.2|P-value:6.99E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL23A(YBL087C)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication Gene:RRM3(YHR031C)|FD-Score:-4.09|P-value:2.19E-5||SGD DESC:DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p Gene:SEC72(YLR292C)|FD-Score:-4.39|P-value:5.75E-6||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SHB17(YKR043C)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SIT4(YDL047W)|FD-Score:5.28|P-value:6.35E-8||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SOH1(YGL127C)|FD-Score:-3.3|P-value:4.83E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SPT3(YDR392W)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:TCB3(YML072C)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TMA64(YDR117C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity Gene:VPS24(YKL041W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS52(YDR484W)|FD-Score:-4.29|P-value:8.98E-6||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:VPS72(YDR485C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:XYL2(YLR070C)|FD-Score:-3.91|P-value:4.70E-5||SGD DESC:Xylitol dehydrogenase, converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect Gene:YDL180W(YDL180W_p)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YDR034W-B(YDR034W-B_p)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR514C(YDR514C)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication Gene:YFL051C(YFL051C_p)|FD-Score:3.22|P-value:6.39E-4||SGD DESC:Putative protein of unknown function; YFL051C is not an essential gene Gene:YIA6(YIL006W)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs Gene:YIL012W(YIL012W_d)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR111C(YJR111C_p)|FD-Score:-3.23|P-value:6.16E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria Gene:YLL053C(YLL053C_p)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin Gene:YME1(YPR024W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Catalytic subunit of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; also has a role in intermembrane space protein folding; mutation causes an elevated rate of mitochondrial turnover Gene:YMR160W(YMR160W_p)|FD-Score:3.79|P-value:7.63E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress Gene:YMR272W-B(YMR272W-B_p)|FD-Score:3.6|P-value:1.56E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YMR306C-A(YMR306C-A_d)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:3.1|P-value:9.56E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL025C(YPL025C_d)|FD-Score:6.24|P-value:2.25E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR063C(YPR063C_p)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:ER-localized protein of unknown function Gene:ACS1(YAL054C)|FD-Score:3.86|P-value:5.76E-5||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions Gene:AHC1(YOR023C)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex Gene:ALG5(YPL227C)|FD-Score:-5.6|P-value:1.08E-8||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:APP1(YNL094W)|FD-Score:3.84|P-value:6.20E-5||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:ATP14(YLR295C)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:BDH2(YAL061W_p)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3 Gene:COG6(YNL041C)|FD-Score:-3.74|P-value:9.16E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DIG1(YPL049C)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:ELP2(YGR200C)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:FRE5(YOR384W)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ILM1(YJR118C)|FD-Score:4.29|P-value:9.00E-6||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:IOC3(YFR013W)|FD-Score:-3.1|P-value:9.67E-4||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:LTE1(YAL024C)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MAC1(YMR021C)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MNN2(YBR015C)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRS3(YJL133W)|FD-Score:4.66|P-value:1.61E-6||SGD DESC:Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication Gene:MSB4(YOL112W)|FD-Score:-3.29|P-value:4.98E-4||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:PAU7(YAR020C)|FD-Score:-3.15|P-value:8.25E-4||SGD DESC:Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme Gene:PIB1(YDR313C)|FD-Score:3.81|P-value:7.02E-5||SGD DESC:RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain Gene:PTC6(YCR079W)|FD-Score:-4.44|P-value:4.54E-6||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase Gene:RGT1(YKL038W)|FD-Score:-5.76|P-value:4.30E-9||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:ROM2(YLR371W)|FD-Score:-3.2|P-value:6.99E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL23A(YBL087C)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication Gene:RRM3(YHR031C)|FD-Score:-4.09|P-value:2.19E-5||SGD DESC:DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p Gene:SEC72(YLR292C)|FD-Score:-4.39|P-value:5.75E-6||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SHB17(YKR043C)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SIT4(YDL047W)|FD-Score:5.28|P-value:6.35E-8||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SOH1(YGL127C)|FD-Score:-3.3|P-value:4.83E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SPT3(YDR392W)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:TCB3(YML072C)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TMA64(YDR117C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity Gene:VPS24(YKL041W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS52(YDR484W)|FD-Score:-4.29|P-value:8.98E-6||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:VPS72(YDR485C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:XYL2(YLR070C)|FD-Score:-3.91|P-value:4.70E-5||SGD DESC:Xylitol dehydrogenase, converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect Gene:YDL180W(YDL180W_p)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YDR034W-B(YDR034W-B_p)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR514C(YDR514C)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication Gene:YFL051C(YFL051C_p)|FD-Score:3.22|P-value:6.39E-4||SGD DESC:Putative protein of unknown function; YFL051C is not an essential gene Gene:YIA6(YIL006W)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs Gene:YIL012W(YIL012W_d)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR111C(YJR111C_p)|FD-Score:-3.23|P-value:6.16E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria Gene:YLL053C(YLL053C_p)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin Gene:YME1(YPR024W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Catalytic subunit of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; also has a role in intermembrane space protein folding; mutation causes an elevated rate of mitochondrial turnover Gene:YMR160W(YMR160W_p)|FD-Score:3.79|P-value:7.63E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress Gene:YMR272W-B(YMR272W-B_p)|FD-Score:3.6|P-value:1.56E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YMR306C-A(YMR306C-A_d)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:3.1|P-value:9.56E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL025C(YPL025C_d)|FD-Score:6.24|P-value:2.25E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR063C(YPR063C_p)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:ER-localized protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR252W6.153.92E-101.12DUT1deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate
YGR265W_d5.032.49E-71.05YGR265W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase
YNL124W3.983.51E-50.67NAF1RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p
YPR183W3.314.68E-40.18DPM1Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation
YER133W3.128.89E-40.15GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YPR165W2.970.001480.06RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YDL102W2.910.001810.03POL3Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)
YJL090C2.880.001990.15DPB11DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress
YDR243C2.730.003210.05PRP28RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site
YDR449C2.680.003700.14UTP6Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YOR063W2.540.005580.00RPL3Ribosomal 60S subunit protein L3; homologous to mammalian ribosomal protein L3 and bacterial L3; involved in the replication and maintenance of killer double stranded RNA virus
YDR325W2.540.005600.01YCG1Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YLR086W2.530.005700.10SMC4Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate
YNR035C2.430.007500.04ARC35Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin
YGR116W2.390.008370.12SPT6Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL025C_d6.242.25E-10YPL025C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDL047W5.286.35E-8SIT4Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization
YJL133W4.661.61E-6MRS3Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication
YJR118C4.299.00E-6ILM1Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth
YOR023C4.221.24E-5AHC1Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex
YDR392W4.201.35E-5SPT3Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
YOR384W4.161.58E-5FRE5Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YAL054C3.865.76E-5ACS1Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions
YNL094W3.846.20E-5APP1Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway
YDR313C3.817.02E-5PIB1RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain
YMR160W_p3.797.63E-5YMR160W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress
YKL041W3.711.05E-4VPS24One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway
YPR063C_p3.691.12E-4YPR063C_pER-localized protein of unknown function
YDR117C3.641.35E-4TMA64Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity
YMR272W-B_p3.601.56E-4YMR272W-B_pIdentified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching

GO enrichment analysis for SGTC_3265
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1288.04E-23SGTC_32629137218 49.5 μMChembridge (Drug-like library)176506640.171875
0.0823.29E-10SGTC_29329016711 44.5 μMChembridge (Drug-like library)71173280.108108
0.0681.50E-7SGTC_390986-0283 58.3 μMChemDiv (Drug-like library)31106000.128571calcium & mitochondrial duress
0.0672.62E-7SGTC_32689137683 49.5 μMChembridge (Drug-like library)19532280.1
0.0664.18E-7SGTC_2545solidagenone 89.6 μMMicrosource (Natural product library)67085720.0875
0.0655.76E-7SGTC_500h-89 112.0 μMICCB bioactive library4492410.0588235cell wall signaling
0.0631.08E-6SGTC_31369095672 49.5 μMChembridge (Drug-like library)172173800.191781
0.0612.64E-6SGTC_1625st003711 30.1 μMTimTec (Natural product derivative library)242070340.10989calcium & mitochondrial duress
0.0613.21E-6SGTC_14113909-8223 150.0 μMChemDiv (Drug-like library)X14110.107143
0.0603.66E-6SGTC_120849-0099 9.3 μMChemDiv (Drug-like library)60713880.0945946
0.0589.41E-6SGTC_6341611-4317 11.2 μMChemDiv (Drug-like library)28435220.161765ERG2
0.0561.54E-5SGTC_10553448-0672 86.0 μMChemDiv (Drug-like library)7483780.174603
0.0561.79E-5SGTC_31079121808 49.5 μMChembridge (Drug-like library)6626810.0625
0.0561.81E-5SGTC_7764476-4378 112.0 μMChemDiv (Drug-like library)28920080.115385ERG2
0.0542.93E-5SGTC_12910993-0025 5.8 μMChemDiv (Drug-like library)43533560.0909091

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1460k062-00599.15 μM0.4107141373085ChemDiv (Drug-like library)368.2328434.31513Golgi
SGTC_1823448-4371550.52 μM0.3709683491403ChemDiv (Drug-like library)363.28095.42211
SGTC_3129912725749.47 μM0.363636837894Chembridge (Drug-like library)249.371763.61812
SGTC_1270180-033359.13 μM0.350877771601ChemDiv (Drug-like library)324.158743.71113mitochondrial response to ROS
SGTC_12971068-011114.1 μM0.345455767057ChemDiv (Drug-like library)312.214244.33712
SGTC_12961068-010211.9 μM0.333333767053ChemDiv (Drug-like library)310.175224.02712
SGTC_3283910933235.82 μM0.33333317027281Chembridge (Drug-like library)329.82393.45913DNA intercalators
SGTC_7211187-1332279 μM0.32075525730ChemDiv (Drug-like library)281.13733.96522cell wall signaling
SGTC_1543389-1638511.12 μM0.3134335238686ChemDiv (Drug-like library)391.2915.29112
SGTC_296090793895.97 μM0.31343316457355Chembridge (Drug-like library)310.775962.36514