9136860

3-phenyl-5-propyl-N-(pyridin-2-ylmethyl)pyrazolo[1,5-a]pyrimidin-7-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3266
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 4871271
SMILES CCCC1=NC2=C(C=NN2C(=C1)NCC3=CC=CC=N3)C4=CC=CC=C4
Standardized SMILES CCCc1cc(NCc2ccccn2)n3ncc(c4ccccc4)c3n1
Molecular weight 343.4249
ALogP 4.02
H-bond donor count 1
H-bond acceptor count 4
Response signature cell wall

Pool Growth Kinetics
% growth inhibition (Het. pool) 25.71
% growth inhibition (Hom. pool) 26.23


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4871271
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:4.3|P-value:8.44E-6|Clearance:0.22||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARP4(YJL081C)|FD-Score:3.4|P-value:3.42E-4|Clearance:0.05||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:CCT3(YJL014W)|FD-Score:4.36|P-value:6.56E-6|Clearance:0.06||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:COG2(YGR120C)|FD-Score:3.24|P-value:5.97E-4|Clearance:0.01||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DED1(YOR204W)|FD-Score:3.81|P-value:7.04E-5|Clearance:0||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:DPM1(YPR183W)|FD-Score:5.2|P-value:9.78E-8|Clearance:0.23||SGD DESC:Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation Gene:ERG11(YHR007C)|FD-Score:6.05|P-value:7.19E-10|Clearance:0.16||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG12(YMR208W)|FD-Score:3.8|P-value:7.12E-5|Clearance:0.27||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:GLC7(YER133W)|FD-Score:3.53|P-value:2.07E-4|Clearance:0.07||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:MCD4(YKL165C)|FD-Score:-3.68|P-value:1.15E-4|Clearance:0||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MCM2(YBL023C)|FD-Score:4.08|P-value:2.24E-5|Clearance:0.19||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:MCM7(YBR202W)|FD-Score:5.57|P-value:1.29E-8|Clearance:0.36||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:MED4(YOR174W)|FD-Score:-4.2|P-value:1.33E-5|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NAB3(YPL190C)|FD-Score:-3.35|P-value:4.01E-4|Clearance:0||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing Gene:NOP8(YOL144W)|FD-Score:3.46|P-value:2.67E-4|Clearance:0.07||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NUP57(YGR119C)|FD-Score:-3.52|P-value:2.20E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:PFY1(YOR122C)|FD-Score:-3.28|P-value:5.14E-4|Clearance:0||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:POL3(YDL102W)|FD-Score:-4.65|P-value:1.64E-6|Clearance:0||SGD DESC:Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) Gene:POP6(YGR030C)|FD-Score:3.21|P-value:6.75E-4|Clearance:0.12||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RHO1(YPR165W)|FD-Score:17.4|P-value:1.82E-68|Clearance:7.9||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RLI1(YDR091C)|FD-Score:-4.18|P-value:1.46E-5|Clearance:0||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RPL33A(YPL143W)|FD-Score:-4.42|P-value:4.86E-6|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:RPL5(YPL131W)|FD-Score:-4.7|P-value:1.31E-6|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPN2(YIL075C)|FD-Score:3.23|P-value:6.23E-4|Clearance:0.02||SGD DESC:Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress Gene:RPN9(YDR427W)|FD-Score:3.28|P-value:5.26E-4|Clearance:0.04||SGD DESC:Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress Gene:RPS13(YDR064W)|FD-Score:-3.43|P-value:2.99E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPS15(YOL040C)|FD-Score:-4.5|P-value:3.39E-6|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPS2(YGL123W)|FD-Score:-3.13|P-value:8.79E-4|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RSC58(YLR033W)|FD-Score:7.56|P-value:1.96E-14|Clearance:1.51||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:3.35|P-value:4.11E-4|Clearance:0.07||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC9(YML127W)|FD-Score:9.55|P-value:6.80E-22|Clearance:1.98||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC21(YNL287W)|FD-Score:-3.28|P-value:5.28E-4|Clearance:0||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SEC26(YDR238C)|FD-Score:-4.4|P-value:5.50E-6|Clearance:0||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SEC5(YDR166C)|FD-Score:-3.9|P-value:4.81E-5|Clearance:0||SGD DESC:Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip Gene:SEN1(YLR430W)|FD-Score:-3.77|P-value:8.28E-5|Clearance:0||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:SPC98(YNL126W)|FD-Score:4.56|P-value:2.60E-6|Clearance:0.2||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SRP102(YKL154W)|FD-Score:-3.11|P-value:9.38E-4|Clearance:0||SGD DESC:Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane Gene:YBR124W(YBR124W_d)|FD-Score:3.89|P-value:4.96E-5|Clearance:0.09||SGD DESC:Putative protein of unknown function Gene:YBR190W(YBR190W_d)|FD-Score:-5.89|P-value:1.89E-9|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YDL196W(YDL196W_d)|FD-Score:-6.97|P-value:1.58E-12|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YDR187C(YDR187C_d)|FD-Score:5.89|P-value:1.95E-9|Clearance:0.32||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YKL111C(YKL111C_d)|FD-Score:4.98|P-value:3.25E-7|Clearance:0.42||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene ABF1 Gene:ACC1(YNR016C)|FD-Score:4.3|P-value:8.44E-6|Clearance:0.22||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARP4(YJL081C)|FD-Score:3.4|P-value:3.42E-4|Clearance:0.05||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:CCT3(YJL014W)|FD-Score:4.36|P-value:6.56E-6|Clearance:0.06||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:COG2(YGR120C)|FD-Score:3.24|P-value:5.97E-4|Clearance:0.01||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DED1(YOR204W)|FD-Score:3.81|P-value:7.04E-5|Clearance:0||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:DPM1(YPR183W)|FD-Score:5.2|P-value:9.78E-8|Clearance:0.23||SGD DESC:Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation Gene:ERG11(YHR007C)|FD-Score:6.05|P-value:7.19E-10|Clearance:0.16||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG12(YMR208W)|FD-Score:3.8|P-value:7.12E-5|Clearance:0.27||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:GLC7(YER133W)|FD-Score:3.53|P-value:2.07E-4|Clearance:0.07||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:MCD4(YKL165C)|FD-Score:-3.68|P-value:1.15E-4|Clearance:0||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MCM2(YBL023C)|FD-Score:4.08|P-value:2.24E-5|Clearance:0.19||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:MCM7(YBR202W)|FD-Score:5.57|P-value:1.29E-8|Clearance:0.36||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:MED4(YOR174W)|FD-Score:-4.2|P-value:1.33E-5|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NAB3(YPL190C)|FD-Score:-3.35|P-value:4.01E-4|Clearance:0||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing Gene:NOP8(YOL144W)|FD-Score:3.46|P-value:2.67E-4|Clearance:0.07||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NUP57(YGR119C)|FD-Score:-3.52|P-value:2.20E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:PFY1(YOR122C)|FD-Score:-3.28|P-value:5.14E-4|Clearance:0||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:POL3(YDL102W)|FD-Score:-4.65|P-value:1.64E-6|Clearance:0||SGD DESC:Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) Gene:POP6(YGR030C)|FD-Score:3.21|P-value:6.75E-4|Clearance:0.12||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RHO1(YPR165W)|FD-Score:17.4|P-value:1.82E-68|Clearance:7.9||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RLI1(YDR091C)|FD-Score:-4.18|P-value:1.46E-5|Clearance:0||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RPL33A(YPL143W)|FD-Score:-4.42|P-value:4.86E-6|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:RPL5(YPL131W)|FD-Score:-4.7|P-value:1.31E-6|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPN2(YIL075C)|FD-Score:3.23|P-value:6.23E-4|Clearance:0.02||SGD DESC:Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress Gene:RPN9(YDR427W)|FD-Score:3.28|P-value:5.26E-4|Clearance:0.04||SGD DESC:Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress Gene:RPS13(YDR064W)|FD-Score:-3.43|P-value:2.99E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPS15(YOL040C)|FD-Score:-4.5|P-value:3.39E-6|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPS2(YGL123W)|FD-Score:-3.13|P-value:8.79E-4|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RSC58(YLR033W)|FD-Score:7.56|P-value:1.96E-14|Clearance:1.51||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:3.35|P-value:4.11E-4|Clearance:0.07||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC9(YML127W)|FD-Score:9.55|P-value:6.80E-22|Clearance:1.98||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC21(YNL287W)|FD-Score:-3.28|P-value:5.28E-4|Clearance:0||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SEC26(YDR238C)|FD-Score:-4.4|P-value:5.50E-6|Clearance:0||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SEC5(YDR166C)|FD-Score:-3.9|P-value:4.81E-5|Clearance:0||SGD DESC:Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip Gene:SEN1(YLR430W)|FD-Score:-3.77|P-value:8.28E-5|Clearance:0||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:SPC98(YNL126W)|FD-Score:4.56|P-value:2.60E-6|Clearance:0.2||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SRP102(YKL154W)|FD-Score:-3.11|P-value:9.38E-4|Clearance:0||SGD DESC:Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane Gene:YBR124W(YBR124W_d)|FD-Score:3.89|P-value:4.96E-5|Clearance:0.09||SGD DESC:Putative protein of unknown function Gene:YBR190W(YBR190W_d)|FD-Score:-5.89|P-value:1.89E-9|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YDL196W(YDL196W_d)|FD-Score:-6.97|P-value:1.58E-12|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YDR187C(YDR187C_d)|FD-Score:5.89|P-value:1.95E-9|Clearance:0.32||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YKL111C(YKL111C_d)|FD-Score:4.98|P-value:3.25E-7|Clearance:0.42||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene ABF1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4871271
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:8.93|P-value:2.11E-19||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AEP2(YMR282C)|FD-Score:-4.83|P-value:6.93E-7||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AEP3(YPL005W)|FD-Score:-4.62|P-value:1.88E-6||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:AIM26(YKL037W)|FD-Score:3.86|P-value:5.67E-5||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:ALG5(YPL227C)|FD-Score:-8.58|P-value:4.59E-18||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:ALG6(YOR002W)|FD-Score:-5.34|P-value:4.67E-8||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:ALG8(YOR067C)|FD-Score:-6.81|P-value:4.73E-12||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:APN1(YKL114C)|FD-Score:5.11|P-value:1.63E-7||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARC18(YLR370C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARG82(YDR173C)|FD-Score:-4.03|P-value:2.79E-5||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ARO1(YDR127W)|FD-Score:-3.75|P-value:9.00E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARP1(YHR129C)|FD-Score:3.97|P-value:3.59E-5||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:ARP5(YNL059C)|FD-Score:3.92|P-value:4.44E-5||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ARP8(YOR141C)|FD-Score:4.63|P-value:1.83E-6||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:ATP7(YKL016C)|FD-Score:-3.3|P-value:4.84E-4||SGD DESC:Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BCK1(YJL095W)|FD-Score:11.9|P-value:3.62E-33||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BEM3(YPL115C)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:BNI4(YNL233W)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BOI1(YBL085W)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:-3.12|P-value:8.91E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD26(YDR241W_d)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUL1(YMR275C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication Gene:BUR2(YLR226W)|FD-Score:-3.19|P-value:7.12E-4||SGD DESC:Cyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2p comprise a CDK-cyclin complex involved in transcriptional regulation through its phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II Gene:CCW12(YLR110C)|FD-Score:-4.62|P-value:1.89E-6||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CHO1(YER026C)|FD-Score:-3.54|P-value:2.03E-4||SGD DESC:Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline Gene:CHS3(YBR023C)|FD-Score:13.7|P-value:3.34E-43||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:CHS5(YLR330W)|FD-Score:6.05|P-value:7.44E-10||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CHS6(YJL099W)|FD-Score:8.23|P-value:9.33E-17||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:CNB1(YKL190W)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:CSF1(YLR087C)|FD-Score:-4.72|P-value:1.16E-6||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CTK3(YML112W)|FD-Score:-3.57|P-value:1.79E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CTR1(YPR124W)|FD-Score:-3.31|P-value:4.60E-4||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CYS3(YAL012W)|FD-Score:-7.55|P-value:2.25E-14||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:CYS4(YGR155W)|FD-Score:-7.37|P-value:8.35E-14||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAL82(YNL314W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain Gene:DCS1(YLR270W)|FD-Score:-3.16|P-value:7.78E-4||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DCV1(YFR012W)|FD-Score:4.99|P-value:2.95E-7||SGD DESC:Protein of unknown function; deletion mutant shows strong genetic interaction with cdc28-as1 mutant in the presence of 1-NM-PP1; DCV1 has a paralog, YOL019W, that arose from the whole genome duplication Gene:DFG5(YMR238W)|FD-Score:-3.13|P-value:8.84E-4||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DIG1(YPL049C)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DOC1(YGL240W)|FD-Score:-3.45|P-value:2.84E-4||SGD DESC:Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain Gene:DRS2(YAL026C)|FD-Score:-4.06|P-value:2.40E-5||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:DSS1(YMR287C)|FD-Score:-3.7|P-value:1.10E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:DUG2(YBR281C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:EMI2(YDR516C)|FD-Score:-3.74|P-value:9.11E-5||SGD DESC:Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:ERF2(YLR246W)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:Subunit of a palmitoyltransferase, composed of Erf2p and Shr5p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole Gene:ERG2(YMR202W)|FD-Score:-6.82|P-value:4.43E-12||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG3(YLR056W)|FD-Score:-4.01|P-value:3.07E-5||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:ERI1(YPL096C-A)|FD-Score:-3.47|P-value:2.65E-4||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:FAB1(YFR019W)|FD-Score:-3.65|P-value:1.32E-4||SGD DESC:1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis Gene:FAT1(YBR041W)|FD-Score:-4.05|P-value:2.55E-5||SGD DESC:Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids Gene:FLC2(YAL053W)|FD-Score:-4.78|P-value:8.90E-7||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:FMP21(YBR269C_p)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FPS1(YLL043W)|FD-Score:5.65|P-value:8.15E-9||SGD DESC:Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress Gene:FYV12(YOR183W_p)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GAL11(YOL051W)|FD-Score:-4.24|P-value:1.13E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GCS1(YDL226C)|FD-Score:-3.14|P-value:8.52E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GET2(YER083C)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GET3(YDL100C)|FD-Score:-6.33|P-value:1.21E-10||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GLY1(YEL046C)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GPD1(YDL022W)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:GPM2(YDL021W)|FD-Score:4.71|P-value:1.21E-6||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:GTR1(YML121W)|FD-Score:4.35|P-value:6.84E-6||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:GTR2(YGR163W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:GUP1(YGL084C)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:GYP1(YOR070C)|FD-Score:-5.72|P-value:5.24E-9||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HFI1(YPL254W)|FD-Score:-3.43|P-value:3.04E-4||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HIT1(YJR055W)|FD-Score:-4.36|P-value:6.44E-6||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HUR1(YGL168W)|FD-Score:-5.67|P-value:6.94E-9||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:IDP1(YDL066W)|FD-Score:9.51|P-value:9.42E-22||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IES6(YEL044W)|FD-Score:5.01|P-value:2.76E-7||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes Gene:IRA2(YOL081W)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication Gene:IRC4(YDR540C)|FD-Score:4.4|P-value:5.46E-6||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:IRS4(YKR019C)|FD-Score:6.68|P-value:1.16E-11||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISA1(YLL027W)|FD-Score:-3.28|P-value:5.21E-4||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:JEM1(YJL073W)|FD-Score:3.93|P-value:4.33E-5||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:JIP3(YLR331C_d)|FD-Score:10.4|P-value:1.40E-25||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2 Gene:KGD2(YDR148C)|FD-Score:-3.97|P-value:3.62E-5||SGD DESC:Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated Gene:LDB16(YCL005W)|FD-Score:5.74|P-value:4.72E-9||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:LDB7(YBL006C)|FD-Score:23|P-value:1.68E-117||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LIP5(YOR196C)|FD-Score:-3.25|P-value:5.77E-4||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:LSM6(YDR378C)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:LST7(YGR057C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MAC1(YMR021C)|FD-Score:-4.19|P-value:1.38E-5||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDM12(YOL009C)|FD-Score:-3.17|P-value:7.59E-4||SGD DESC:Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins Gene:MEH1(YKR007W)|FD-Score:5.97|P-value:1.20E-9||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MET32(YDR253C)|FD-Score:4.42|P-value:4.90E-6||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication Gene:MHR1(YDR296W)|FD-Score:-3.16|P-value:7.79E-4||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MID2(YLR332W)|FD-Score:4.96|P-value:3.59E-7||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MMM1(YLL006W)|FD-Score:-6.09|P-value:5.73E-10||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRP21(YBL090W)|FD-Score:-4.58|P-value:2.29E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPL11(YDL202W)|FD-Score:-3.38|P-value:3.68E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL22(YNL177C)|FD-Score:-4.09|P-value:2.14E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL28(YDR462W)|FD-Score:-4.01|P-value:3.02E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPL35(YDR322W)|FD-Score:-5.35|P-value:4.44E-8||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL4(YLR439W)|FD-Score:-5.18|P-value:1.08E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MRPL51(YPR100W)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL8(YJL063C)|FD-Score:-3.65|P-value:1.32E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS12(YNR036C)|FD-Score:-4.6|P-value:2.10E-6||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MRPS35(YGR165W)|FD-Score:-5.02|P-value:2.58E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MRPS5(YBR251W)|FD-Score:-3.18|P-value:7.49E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSC1(YML128C)|FD-Score:8.05|P-value:4.15E-16||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated Gene:MSS116(YDR194C)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing Gene:MSW1(YDR268W)|FD-Score:-6.02|P-value:8.92E-10||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MTF2(YDL044C)|FD-Score:-4.33|P-value:7.43E-6||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:MTG1(YMR097C)|FD-Score:-5.46|P-value:2.40E-8||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:MTO1(YGL236C)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants Gene:NAB6(YML117W)|FD-Score:4.09|P-value:2.16E-5||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NAT3(YPR131C)|FD-Score:-3.38|P-value:3.68E-4||SGD DESC:Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met Gene:NHX1(YDR456W)|FD-Score:-3.75|P-value:8.69E-5||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:NOP6(YDL213C)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes Gene:OCT1(YKL134C)|FD-Score:-6.42|P-value:6.79E-11||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:OPI3(YJR073C)|FD-Score:-3.22|P-value:6.44E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OSH3(YHR073W)|FD-Score:3.89|P-value:5.11E-5||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PDR1(YGL013C)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEP12(YOR036W)|FD-Score:-3.23|P-value:6.09E-4||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP3(YLR148W)|FD-Score:-6.61|P-value:1.93E-11||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEP7(YDR323C)|FD-Score:-6.06|P-value:6.77E-10||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PER1(YCR044C)|FD-Score:-4.45|P-value:4.35E-6||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PET111(YMR257C)|FD-Score:-3.97|P-value:3.53E-5||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PET309(YLR067C)|FD-Score:-3.22|P-value:6.33E-4||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PGD1(YGL025C)|FD-Score:-5.51|P-value:1.76E-8||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PIF1(YML061C)|FD-Score:-4.41|P-value:5.15E-6||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PIM1(YBL022C)|FD-Score:5.08|P-value:1.88E-7||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PKH2(YOL100W)|FD-Score:-4.01|P-value:3.07E-5||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PSY1(YKL076C_d)|FD-Score:4.44|P-value:4.54E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C Gene:RAI1(YGL246C)|FD-Score:-3.85|P-value:5.86E-5||SGD DESC:Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z Gene:RAM1(YDL090C)|FD-Score:-4.4|P-value:5.39E-6||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RCY1(YJL204C)|FD-Score:-4|P-value:3.20E-5||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:REG1(YDR028C)|FD-Score:-5.64|P-value:8.58E-9||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RGD1(YBR260C)|FD-Score:3.12|P-value:8.90E-4||SGD DESC:GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization Gene:RIM1(YCR028C-A)|FD-Score:-7.08|P-value:7.39E-13||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RNR4(YGR180C)|FD-Score:-3.78|P-value:7.93E-5||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ROM2(YLR371W)|FD-Score:10.9|P-value:4.35E-28||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPA12(YJR063W)|FD-Score:-3.22|P-value:6.32E-4||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPL20A(YMR242C)|FD-Score:5.39|P-value:3.47E-8||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPL20B(YOR312C)|FD-Score:-5.36|P-value:4.24E-8||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPL21B(YPL079W)|FD-Score:-3.42|P-value:3.10E-4||SGD DESC:Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:-3.83|P-value:6.37E-5||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPP1A(YDL081C)|FD-Score:-3.94|P-value:4.12E-5||SGD DESC:Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component Gene:RPS0B(YLR048W)|FD-Score:-5.56|P-value:1.38E-8||SGD DESC:Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2 Gene:RPS16A(YMR143W)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication Gene:RPS18A(YDR450W)|FD-Score:-3.2|P-value:6.95E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:RPS29B(YDL061C)|FD-Score:-3.36|P-value:3.95E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication Gene:RPS4B(YHR203C)|FD-Score:-4.18|P-value:1.44E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication Gene:RRT12(YCR045C)|FD-Score:4.42|P-value:4.96E-6||SGD DESC:Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores Gene:RSA1(YPL193W)|FD-Score:-7.07|P-value:7.75E-13||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:RSC1(YGR056W)|FD-Score:3.79|P-value:7.49E-5||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:RSM18(YER050C)|FD-Score:-7.87|P-value:1.80E-15||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:RSM27(YGR215W)|FD-Score:-4.28|P-value:9.45E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTC6(YPL183W-A)|FD-Score:-4.34|P-value:7.26E-6||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:RXT2(YBR095C)|FD-Score:-3.35|P-value:4.10E-4||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SAC1(YKL212W)|FD-Score:-5.3|P-value:5.72E-8||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SCP160(YJL080C)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SEC22(YLR268W)|FD-Score:-3.48|P-value:2.47E-4||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SEH1(YGL100W)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SEM1(YDR363W-A)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress Gene:SHE1(YBL031W)|FD-Score:5.73|P-value:5.12E-9||SGD DESC:Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function Gene:SIN4(YNL236W)|FD-Score:-5.67|P-value:7.16E-9||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SKN7(YHR206W)|FD-Score:-3.56|P-value:1.83E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SKT5(YBL061C)|FD-Score:4.97|P-value:3.28E-7||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:SLS1(YLR139C)|FD-Score:-4.41|P-value:5.07E-6||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SLT2(YHR030C)|FD-Score:9.67|P-value:2.11E-22||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SLX8(YER116C)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:SLX9(YGR081C)|FD-Score:-4.35|P-value:6.93E-6||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SNF2(YOR290C)|FD-Score:-3.28|P-value:5.20E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SNF7(YLR025W)|FD-Score:-7.29|P-value:1.55E-13||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNF8(YPL002C)|FD-Score:-3.27|P-value:5.47E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SOH1(YGL127C)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SPT3(YDR392W)|FD-Score:-6.1|P-value:5.23E-10||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SPT8(YLR055C)|FD-Score:-4.28|P-value:9.33E-6||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:SSD1(YDR293C)|FD-Score:7.07|P-value:7.63E-13||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSN2(YDR443C)|FD-Score:-4.75|P-value:9.99E-7||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SSN3(YPL042C)|FD-Score:-6.43|P-value:6.30E-11||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:STP22(YCL008C)|FD-Score:-3.27|P-value:5.40E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SWI6(YLR182W)|FD-Score:-3.87|P-value:5.49E-5||SGD DESC:Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators Gene:SWS2(YNL081C)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TDA5(YLR426W_p)|FD-Score:3.77|P-value:8.04E-5||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TED1(YIL039W)|FD-Score:-5.51|P-value:1.83E-8||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TIF4632(YGL049C)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); TIF4632 has a paralog, TIF4631, that arose from the whole genome duplication Gene:TOM7(YNL070W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TOS1(YBR162C)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C Gene:TRK1(YJL129C)|FD-Score:-3.66|P-value:1.24E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TRS33(YOR115C)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TRS65(YGR166W)|FD-Score:-4.53|P-value:2.94E-6||SGD DESC:Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Gene:TUF1(YOR187W)|FD-Score:-3.18|P-value:7.27E-4||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:UBC12(YLR306W)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Enzyme that mediates the conjugation of Rub1p, a ubiquitin-like protein, to other proteins; related to E2 ubiquitin-conjugating enzymes Gene:UBP3(YER151C)|FD-Score:-4.57|P-value:2.46E-6||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:UBP8(YMR223W)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B Gene:URE2(YNL229C)|FD-Score:13|P-value:5.32E-39||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VMA1(YDL185W)|FD-Score:-4.69|P-value:1.37E-6||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA11(YPL234C)|FD-Score:-6.79|P-value:5.53E-12||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:VMA13(YPR036W)|FD-Score:-4.66|P-value:1.56E-6||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA16(YHR026W)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VMA9(YCL005W-A)|FD-Score:-3.15|P-value:8.13E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VOA1(YGR106C)|FD-Score:4.41|P-value:5.11E-6||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:VPS1(YKR001C)|FD-Score:-4.32|P-value:7.95E-6||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS20(YMR077C)|FD-Score:-6.81|P-value:4.76E-12||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS24(YKL041W)|FD-Score:-6.07|P-value:6.25E-10||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS25(YJR102C)|FD-Score:-3.98|P-value:3.49E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS27(YNR006W)|FD-Score:-3.43|P-value:2.97E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS28(YPL065W)|FD-Score:-3.86|P-value:5.59E-5||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS3(YDR495C)|FD-Score:-3.11|P-value:9.27E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS36(YLR417W)|FD-Score:-3.87|P-value:5.42E-5||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS4(YPR173C)|FD-Score:-3.25|P-value:5.83E-4||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:VPS45(YGL095C)|FD-Score:-8.11|P-value:2.50E-16||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:WSC2(YNL283C)|FD-Score:5.38|P-value:3.64E-8||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YBL062W(YBL062W_d)|FD-Score:4.59|P-value:2.21E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL083C(YBL083C_d)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YBR292C(YBR292C_d)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene Gene:YBR296C-A(YBR296C-A_p)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDL011C(YDL011C_d)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W Gene:YDL041W(YDL041W_d)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDR114C(YDR114C_p)|FD-Score:-5.57|P-value:1.31E-8||SGD DESC:Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium Gene:YDR149C(YDR149C_d)|FD-Score:5.51|P-value:1.76E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YDR161W(YDR161W_p)|FD-Score:-3.17|P-value:7.61E-4||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YDR203W(YDR203W_d)|FD-Score:-4.17|P-value:1.55E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR509W(YDR509W_d)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR524C-B(YDR524C-B_p)|FD-Score:-4.43|P-value:4.76E-6||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YER077C(YER077C_p)|FD-Score:-4.61|P-value:2.00E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YER119C-A(YER119C-A_d)|FD-Score:9.03|P-value:8.57E-20||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YGR064W(YGR064W_d)|FD-Score:-7.23|P-value:2.45E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YGR160W(YGR160W_d)|FD-Score:-4.85|P-value:6.15E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIM1(YMR152W)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:YJL206C(YJL206C_p)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Putative protein of unknown function; similar to transcriptional regulators from the Zn[2]-Cys[6] binuclear cluster protein family; mRNA is weakly cell cycle regulated, peaking in S phase; induced rapidly upon MMS treatment Gene:YJR111C(YJR111C_p)|FD-Score:5.11|P-value:1.64E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria Gene:YJR142W(YJR142W_p)|FD-Score:5.24|P-value:8.18E-8||SGD DESC:Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member Gene:YKL096C-B(YKL096C-B_p)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YKL169C(YKL169C_d)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLL059C(YLL059C_d)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR124W(YMR124W_p)|FD-Score:8.86|P-value:3.83E-19||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization Gene:YMR247W-A(YMR247W-A_p)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Putative protein of unknown function Gene:YNL184C(YNL184C_p)|FD-Score:-3.56|P-value:1.83E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR331C(YOR331C_d)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W Gene:YPK1(YKL126W)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL185W(YPL185W_d)|FD-Score:-3.19|P-value:7.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C Gene:YPL205C(YPL205C_d)|FD-Score:-3.17|P-value:7.53E-4||SGD DESC:Hypothetical protein; deletion of locus affects telomere length Gene:YPR099C(YPR099C_d)|FD-Score:-3.72|P-value:1.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W Gene:YPR123C(YPR123C_d)|FD-Score:-5.57|P-value:1.26E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPR197C(YPR197C_d)|FD-Score:-3.1|P-value:9.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT31(YER031C)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:ACB1(YGR037C)|FD-Score:8.93|P-value:2.11E-19||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AEP2(YMR282C)|FD-Score:-4.83|P-value:6.93E-7||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AEP3(YPL005W)|FD-Score:-4.62|P-value:1.88E-6||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:AIM26(YKL037W)|FD-Score:3.86|P-value:5.67E-5||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:ALG5(YPL227C)|FD-Score:-8.58|P-value:4.59E-18||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:ALG6(YOR002W)|FD-Score:-5.34|P-value:4.67E-8||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:ALG8(YOR067C)|FD-Score:-6.81|P-value:4.73E-12||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:APN1(YKL114C)|FD-Score:5.11|P-value:1.63E-7||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARC18(YLR370C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARG82(YDR173C)|FD-Score:-4.03|P-value:2.79E-5||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ARO1(YDR127W)|FD-Score:-3.75|P-value:9.00E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARP1(YHR129C)|FD-Score:3.97|P-value:3.59E-5||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:ARP5(YNL059C)|FD-Score:3.92|P-value:4.44E-5||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ARP8(YOR141C)|FD-Score:4.63|P-value:1.83E-6||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:ATP7(YKL016C)|FD-Score:-3.3|P-value:4.84E-4||SGD DESC:Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BCK1(YJL095W)|FD-Score:11.9|P-value:3.62E-33||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BEM3(YPL115C)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:BNI4(YNL233W)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BOI1(YBL085W)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:-3.12|P-value:8.91E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD26(YDR241W_d)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUL1(YMR275C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication Gene:BUR2(YLR226W)|FD-Score:-3.19|P-value:7.12E-4||SGD DESC:Cyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2p comprise a CDK-cyclin complex involved in transcriptional regulation through its phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II Gene:CCW12(YLR110C)|FD-Score:-4.62|P-value:1.89E-6||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CHO1(YER026C)|FD-Score:-3.54|P-value:2.03E-4||SGD DESC:Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline Gene:CHS3(YBR023C)|FD-Score:13.7|P-value:3.34E-43||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:CHS5(YLR330W)|FD-Score:6.05|P-value:7.44E-10||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CHS6(YJL099W)|FD-Score:8.23|P-value:9.33E-17||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:CNB1(YKL190W)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:CSF1(YLR087C)|FD-Score:-4.72|P-value:1.16E-6||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CTK3(YML112W)|FD-Score:-3.57|P-value:1.79E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CTR1(YPR124W)|FD-Score:-3.31|P-value:4.60E-4||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CYS3(YAL012W)|FD-Score:-7.55|P-value:2.25E-14||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:CYS4(YGR155W)|FD-Score:-7.37|P-value:8.35E-14||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAL82(YNL314W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain Gene:DCS1(YLR270W)|FD-Score:-3.16|P-value:7.78E-4||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DCV1(YFR012W)|FD-Score:4.99|P-value:2.95E-7||SGD DESC:Protein of unknown function; deletion mutant shows strong genetic interaction with cdc28-as1 mutant in the presence of 1-NM-PP1; DCV1 has a paralog, YOL019W, that arose from the whole genome duplication Gene:DFG5(YMR238W)|FD-Score:-3.13|P-value:8.84E-4||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DIG1(YPL049C)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DOC1(YGL240W)|FD-Score:-3.45|P-value:2.84E-4||SGD DESC:Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain Gene:DRS2(YAL026C)|FD-Score:-4.06|P-value:2.40E-5||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:DSS1(YMR287C)|FD-Score:-3.7|P-value:1.10E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:DUG2(YBR281C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:EMI2(YDR516C)|FD-Score:-3.74|P-value:9.11E-5||SGD DESC:Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:ERF2(YLR246W)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:Subunit of a palmitoyltransferase, composed of Erf2p and Shr5p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole Gene:ERG2(YMR202W)|FD-Score:-6.82|P-value:4.43E-12||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG3(YLR056W)|FD-Score:-4.01|P-value:3.07E-5||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:ERI1(YPL096C-A)|FD-Score:-3.47|P-value:2.65E-4||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:FAB1(YFR019W)|FD-Score:-3.65|P-value:1.32E-4||SGD DESC:1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis Gene:FAT1(YBR041W)|FD-Score:-4.05|P-value:2.55E-5||SGD DESC:Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids Gene:FLC2(YAL053W)|FD-Score:-4.78|P-value:8.90E-7||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:FMP21(YBR269C_p)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FPS1(YLL043W)|FD-Score:5.65|P-value:8.15E-9||SGD DESC:Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress Gene:FYV12(YOR183W_p)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GAL11(YOL051W)|FD-Score:-4.24|P-value:1.13E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GCS1(YDL226C)|FD-Score:-3.14|P-value:8.52E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GET2(YER083C)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GET3(YDL100C)|FD-Score:-6.33|P-value:1.21E-10||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GLY1(YEL046C)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GPD1(YDL022W)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:GPM2(YDL021W)|FD-Score:4.71|P-value:1.21E-6||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:GTR1(YML121W)|FD-Score:4.35|P-value:6.84E-6||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:GTR2(YGR163W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:GUP1(YGL084C)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:GYP1(YOR070C)|FD-Score:-5.72|P-value:5.24E-9||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HFI1(YPL254W)|FD-Score:-3.43|P-value:3.04E-4||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HIT1(YJR055W)|FD-Score:-4.36|P-value:6.44E-6||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HUR1(YGL168W)|FD-Score:-5.67|P-value:6.94E-9||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:IDP1(YDL066W)|FD-Score:9.51|P-value:9.42E-22||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IES6(YEL044W)|FD-Score:5.01|P-value:2.76E-7||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes Gene:IRA2(YOL081W)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication Gene:IRC4(YDR540C)|FD-Score:4.4|P-value:5.46E-6||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:IRS4(YKR019C)|FD-Score:6.68|P-value:1.16E-11||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISA1(YLL027W)|FD-Score:-3.28|P-value:5.21E-4||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:JEM1(YJL073W)|FD-Score:3.93|P-value:4.33E-5||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:JIP3(YLR331C_d)|FD-Score:10.4|P-value:1.40E-25||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2 Gene:KGD2(YDR148C)|FD-Score:-3.97|P-value:3.62E-5||SGD DESC:Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated Gene:LDB16(YCL005W)|FD-Score:5.74|P-value:4.72E-9||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:LDB7(YBL006C)|FD-Score:23|P-value:1.68E-117||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LIP5(YOR196C)|FD-Score:-3.25|P-value:5.77E-4||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:LSM6(YDR378C)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:LST7(YGR057C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MAC1(YMR021C)|FD-Score:-4.19|P-value:1.38E-5||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDM12(YOL009C)|FD-Score:-3.17|P-value:7.59E-4||SGD DESC:Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins Gene:MEH1(YKR007W)|FD-Score:5.97|P-value:1.20E-9||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MET32(YDR253C)|FD-Score:4.42|P-value:4.90E-6||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication Gene:MHR1(YDR296W)|FD-Score:-3.16|P-value:7.79E-4||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MID2(YLR332W)|FD-Score:4.96|P-value:3.59E-7||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MMM1(YLL006W)|FD-Score:-6.09|P-value:5.73E-10||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRP21(YBL090W)|FD-Score:-4.58|P-value:2.29E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPL11(YDL202W)|FD-Score:-3.38|P-value:3.68E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL22(YNL177C)|FD-Score:-4.09|P-value:2.14E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL28(YDR462W)|FD-Score:-4.01|P-value:3.02E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPL35(YDR322W)|FD-Score:-5.35|P-value:4.44E-8||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL4(YLR439W)|FD-Score:-5.18|P-value:1.08E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MRPL51(YPR100W)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL8(YJL063C)|FD-Score:-3.65|P-value:1.32E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS12(YNR036C)|FD-Score:-4.6|P-value:2.10E-6||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MRPS35(YGR165W)|FD-Score:-5.02|P-value:2.58E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MRPS5(YBR251W)|FD-Score:-3.18|P-value:7.49E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSC1(YML128C)|FD-Score:8.05|P-value:4.15E-16||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated Gene:MSS116(YDR194C)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing Gene:MSW1(YDR268W)|FD-Score:-6.02|P-value:8.92E-10||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MTF2(YDL044C)|FD-Score:-4.33|P-value:7.43E-6||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:MTG1(YMR097C)|FD-Score:-5.46|P-value:2.40E-8||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:MTO1(YGL236C)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants Gene:NAB6(YML117W)|FD-Score:4.09|P-value:2.16E-5||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NAT3(YPR131C)|FD-Score:-3.38|P-value:3.68E-4||SGD DESC:Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met Gene:NHX1(YDR456W)|FD-Score:-3.75|P-value:8.69E-5||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:NOP6(YDL213C)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes Gene:OCT1(YKL134C)|FD-Score:-6.42|P-value:6.79E-11||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:OPI3(YJR073C)|FD-Score:-3.22|P-value:6.44E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OSH3(YHR073W)|FD-Score:3.89|P-value:5.11E-5||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PDR1(YGL013C)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEP12(YOR036W)|FD-Score:-3.23|P-value:6.09E-4||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP3(YLR148W)|FD-Score:-6.61|P-value:1.93E-11||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEP7(YDR323C)|FD-Score:-6.06|P-value:6.77E-10||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PER1(YCR044C)|FD-Score:-4.45|P-value:4.35E-6||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PET111(YMR257C)|FD-Score:-3.97|P-value:3.53E-5||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PET309(YLR067C)|FD-Score:-3.22|P-value:6.33E-4||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PGD1(YGL025C)|FD-Score:-5.51|P-value:1.76E-8||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PIF1(YML061C)|FD-Score:-4.41|P-value:5.15E-6||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PIM1(YBL022C)|FD-Score:5.08|P-value:1.88E-7||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PKH2(YOL100W)|FD-Score:-4.01|P-value:3.07E-5||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PSY1(YKL076C_d)|FD-Score:4.44|P-value:4.54E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C Gene:RAI1(YGL246C)|FD-Score:-3.85|P-value:5.86E-5||SGD DESC:Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z Gene:RAM1(YDL090C)|FD-Score:-4.4|P-value:5.39E-6||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RCY1(YJL204C)|FD-Score:-4|P-value:3.20E-5||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:REG1(YDR028C)|FD-Score:-5.64|P-value:8.58E-9||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RGD1(YBR260C)|FD-Score:3.12|P-value:8.90E-4||SGD DESC:GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization Gene:RIM1(YCR028C-A)|FD-Score:-7.08|P-value:7.39E-13||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RNR4(YGR180C)|FD-Score:-3.78|P-value:7.93E-5||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ROM2(YLR371W)|FD-Score:10.9|P-value:4.35E-28||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPA12(YJR063W)|FD-Score:-3.22|P-value:6.32E-4||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPL20A(YMR242C)|FD-Score:5.39|P-value:3.47E-8||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPL20B(YOR312C)|FD-Score:-5.36|P-value:4.24E-8||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPL21B(YPL079W)|FD-Score:-3.42|P-value:3.10E-4||SGD DESC:Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:-3.83|P-value:6.37E-5||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPP1A(YDL081C)|FD-Score:-3.94|P-value:4.12E-5||SGD DESC:Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component Gene:RPS0B(YLR048W)|FD-Score:-5.56|P-value:1.38E-8||SGD DESC:Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2 Gene:RPS16A(YMR143W)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication Gene:RPS18A(YDR450W)|FD-Score:-3.2|P-value:6.95E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:RPS29B(YDL061C)|FD-Score:-3.36|P-value:3.95E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication Gene:RPS4B(YHR203C)|FD-Score:-4.18|P-value:1.44E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication Gene:RRT12(YCR045C)|FD-Score:4.42|P-value:4.96E-6||SGD DESC:Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores Gene:RSA1(YPL193W)|FD-Score:-7.07|P-value:7.75E-13||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:RSC1(YGR056W)|FD-Score:3.79|P-value:7.49E-5||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:RSM18(YER050C)|FD-Score:-7.87|P-value:1.80E-15||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:RSM27(YGR215W)|FD-Score:-4.28|P-value:9.45E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTC6(YPL183W-A)|FD-Score:-4.34|P-value:7.26E-6||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:RXT2(YBR095C)|FD-Score:-3.35|P-value:4.10E-4||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SAC1(YKL212W)|FD-Score:-5.3|P-value:5.72E-8||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SCP160(YJL080C)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SEC22(YLR268W)|FD-Score:-3.48|P-value:2.47E-4||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SEH1(YGL100W)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SEM1(YDR363W-A)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress Gene:SHE1(YBL031W)|FD-Score:5.73|P-value:5.12E-9||SGD DESC:Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function Gene:SIN4(YNL236W)|FD-Score:-5.67|P-value:7.16E-9||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SKN7(YHR206W)|FD-Score:-3.56|P-value:1.83E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SKT5(YBL061C)|FD-Score:4.97|P-value:3.28E-7||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:SLS1(YLR139C)|FD-Score:-4.41|P-value:5.07E-6||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SLT2(YHR030C)|FD-Score:9.67|P-value:2.11E-22||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SLX8(YER116C)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:SLX9(YGR081C)|FD-Score:-4.35|P-value:6.93E-6||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SNF2(YOR290C)|FD-Score:-3.28|P-value:5.20E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SNF7(YLR025W)|FD-Score:-7.29|P-value:1.55E-13||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNF8(YPL002C)|FD-Score:-3.27|P-value:5.47E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SOH1(YGL127C)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SPT3(YDR392W)|FD-Score:-6.1|P-value:5.23E-10||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SPT8(YLR055C)|FD-Score:-4.28|P-value:9.33E-6||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:SSD1(YDR293C)|FD-Score:7.07|P-value:7.63E-13||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSN2(YDR443C)|FD-Score:-4.75|P-value:9.99E-7||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SSN3(YPL042C)|FD-Score:-6.43|P-value:6.30E-11||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:STP22(YCL008C)|FD-Score:-3.27|P-value:5.40E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SWI6(YLR182W)|FD-Score:-3.87|P-value:5.49E-5||SGD DESC:Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators Gene:SWS2(YNL081C)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TDA5(YLR426W_p)|FD-Score:3.77|P-value:8.04E-5||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TED1(YIL039W)|FD-Score:-5.51|P-value:1.83E-8||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TIF4632(YGL049C)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); TIF4632 has a paralog, TIF4631, that arose from the whole genome duplication Gene:TOM7(YNL070W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TOS1(YBR162C)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C Gene:TRK1(YJL129C)|FD-Score:-3.66|P-value:1.24E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TRS33(YOR115C)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TRS65(YGR166W)|FD-Score:-4.53|P-value:2.94E-6||SGD DESC:Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Gene:TUF1(YOR187W)|FD-Score:-3.18|P-value:7.27E-4||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:UBC12(YLR306W)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Enzyme that mediates the conjugation of Rub1p, a ubiquitin-like protein, to other proteins; related to E2 ubiquitin-conjugating enzymes Gene:UBP3(YER151C)|FD-Score:-4.57|P-value:2.46E-6||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:UBP8(YMR223W)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B Gene:URE2(YNL229C)|FD-Score:13|P-value:5.32E-39||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VMA1(YDL185W)|FD-Score:-4.69|P-value:1.37E-6||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA11(YPL234C)|FD-Score:-6.79|P-value:5.53E-12||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:VMA13(YPR036W)|FD-Score:-4.66|P-value:1.56E-6||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA16(YHR026W)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VMA9(YCL005W-A)|FD-Score:-3.15|P-value:8.13E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VOA1(YGR106C)|FD-Score:4.41|P-value:5.11E-6||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:VPS1(YKR001C)|FD-Score:-4.32|P-value:7.95E-6||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS20(YMR077C)|FD-Score:-6.81|P-value:4.76E-12||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS24(YKL041W)|FD-Score:-6.07|P-value:6.25E-10||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS25(YJR102C)|FD-Score:-3.98|P-value:3.49E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS27(YNR006W)|FD-Score:-3.43|P-value:2.97E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS28(YPL065W)|FD-Score:-3.86|P-value:5.59E-5||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS3(YDR495C)|FD-Score:-3.11|P-value:9.27E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS36(YLR417W)|FD-Score:-3.87|P-value:5.42E-5||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS4(YPR173C)|FD-Score:-3.25|P-value:5.83E-4||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:VPS45(YGL095C)|FD-Score:-8.11|P-value:2.50E-16||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:WSC2(YNL283C)|FD-Score:5.38|P-value:3.64E-8||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YBL062W(YBL062W_d)|FD-Score:4.59|P-value:2.21E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL083C(YBL083C_d)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YBR292C(YBR292C_d)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene Gene:YBR296C-A(YBR296C-A_p)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDL011C(YDL011C_d)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W Gene:YDL041W(YDL041W_d)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDR114C(YDR114C_p)|FD-Score:-5.57|P-value:1.31E-8||SGD DESC:Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium Gene:YDR149C(YDR149C_d)|FD-Score:5.51|P-value:1.76E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YDR161W(YDR161W_p)|FD-Score:-3.17|P-value:7.61E-4||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YDR203W(YDR203W_d)|FD-Score:-4.17|P-value:1.55E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR509W(YDR509W_d)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR524C-B(YDR524C-B_p)|FD-Score:-4.43|P-value:4.76E-6||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YER077C(YER077C_p)|FD-Score:-4.61|P-value:2.00E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YER119C-A(YER119C-A_d)|FD-Score:9.03|P-value:8.57E-20||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YGR064W(YGR064W_d)|FD-Score:-7.23|P-value:2.45E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YGR160W(YGR160W_d)|FD-Score:-4.85|P-value:6.15E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIM1(YMR152W)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:YJL206C(YJL206C_p)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Putative protein of unknown function; similar to transcriptional regulators from the Zn[2]-Cys[6] binuclear cluster protein family; mRNA is weakly cell cycle regulated, peaking in S phase; induced rapidly upon MMS treatment Gene:YJR111C(YJR111C_p)|FD-Score:5.11|P-value:1.64E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria Gene:YJR142W(YJR142W_p)|FD-Score:5.24|P-value:8.18E-8||SGD DESC:Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member Gene:YKL096C-B(YKL096C-B_p)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YKL169C(YKL169C_d)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLL059C(YLL059C_d)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR124W(YMR124W_p)|FD-Score:8.86|P-value:3.83E-19||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization Gene:YMR247W-A(YMR247W-A_p)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Putative protein of unknown function Gene:YNL184C(YNL184C_p)|FD-Score:-3.56|P-value:1.83E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR331C(YOR331C_d)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W Gene:YPK1(YKL126W)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL185W(YPL185W_d)|FD-Score:-3.19|P-value:7.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C Gene:YPL205C(YPL205C_d)|FD-Score:-3.17|P-value:7.53E-4||SGD DESC:Hypothetical protein; deletion of locus affects telomere length Gene:YPR099C(YPR099C_d)|FD-Score:-3.72|P-value:1.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W Gene:YPR123C(YPR123C_d)|FD-Score:-5.57|P-value:1.26E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPR197C(YPR197C_d)|FD-Score:-3.1|P-value:9.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT31(YER031C)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR165W17.401.82E-687.90RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YML127W9.556.80E-221.98RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YLR033W7.561.96E-141.51RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YHR007C6.057.19E-100.16ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YDR187C_d5.891.95E-90.32YDR187C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W
YBR202W5.571.29E-80.36MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
YPR183W5.209.78E-80.23DPM1Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation
YKL111C_d4.983.25E-70.42YKL111C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene ABF1
YNL126W4.562.60E-60.20SPC98Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YJL014W4.366.56E-60.06CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YNR016C4.308.44E-60.22ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YBL023C4.082.24E-50.19MCM2Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress
YBR124W_d3.894.96E-50.09YBR124W_dPutative protein of unknown function
YOR204W3.817.04E-50.00DED1ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility
YMR208W3.807.12E-50.27ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBL006C23.001.68E-117LDB7Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions
YBR023C13.703.34E-43CHS3Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
YNL229C13.005.32E-39URE2Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion
YJL095W11.903.62E-33BCK1Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
YLR371W10.904.35E-28ROM2GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p
YLR331C_d10.401.40E-25JIP3_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
YHR030C9.672.11E-22SLT2Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
YDL066W9.519.42E-22IDP1Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes
YER119C-A_d9.038.57E-20YER119C-A_dDubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2
YGR037C8.932.11E-19ACB1Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress
YMR124W_p8.863.83E-19YMR124W_pProtein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
YJL099W8.239.33E-17CHS6Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
YML128C8.054.15E-16MSC1Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated
YDR293C7.077.63E-13SSD1Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function
YKR019C6.681.16E-11IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication

GO enrichment analysis for SGTC_3266
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4503.49E-292SGTC_33119131146 2.9 μMChembridge (Drug-like library)49004060.714286cell wall
0.4281.75E-261SGTC_8630466-0191 129.0 μMChemDiv (Drug-like library)7651600.0921053cell wall
0.3791.74E-200SGTC_33219137793 26.8 μMChembridge (Drug-like library)49041920.342466cell wall
0.3656.93E-186SGTC_3041310-0298 97.0 μMChemDiv (Drug-like library)15502340.0649351cell wall
0.3571.03E-176SGTC_1380850-0259 40.9 μMChemDiv (Drug-like library)68130040.1cell wall
0.3492.52E-168SGTC_33509150499 3.6 μMChembridge (Drug-like library)49109960.364865cell wall
0.3476.21E-167SGTC_2584androsterone 48.7 μMMicrosource (Natural product library)27541300.0238095cell wall
0.3461.94E-165SGTC_9511171-0579 82.7 μMChemDiv (Drug-like library)16160500.060241cell wall
0.3285.42E-148SGTC_29127964298 10.9 μMChembridge (Drug-like library)29758500.102564cell wall
0.3131.63E-134SGTC_33229139058 2.1 μMChembridge (Drug-like library)49089260.719298cell wall
0.3017.64E-124SGTC_6660272-0154 136.0 μMChemDiv (Drug-like library)68703120.139241cell wall
0.2993.29E-122SGTC_33319141060 2.0 μMChembridge (Drug-like library)49065670.426471cell wall
0.2942.26E-118SGTC_32859112818 20.9 μMChembridge (Drug-like library)170179470.0898876cell wall
0.2937.16E-117SGTC_33569151811 4.5 μMChembridge (Drug-like library)18592540.492537cell wall
0.2884.89E-113SGTC_3600957-0399 1.0 μMChemDiv (Drug-like library)67523010.0851064cell wall

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_332291390582.11 μM0.7192984908926Chembridge (Drug-like library)343.424943.80714cell wall
SGTC_331191311462.88 μM0.7142864900406Chembridge (Drug-like library)343.424944.02914cell wall
SGTC_3276913906049.47 μM0.5714294870619Chembridge (Drug-like library)346.428883.6551560S ribosome export
SGTC_335691518114.48 μM0.4925371859254Chembridge (Drug-like library)357.451524.0914cell wall
SGTC_3340914663411.1 μM0.4776124907387Chembridge (Drug-like library)365.471983.14615
SGTC_333191410601.96 μM0.4264714906567Chembridge (Drug-like library)363.843423.84614cell wall
SGTC_3342914779114.51 μM0.4264711838168Chembridge (Drug-like library)349.816843.34914
SGTC_3351915031221.6 μM0.4029854904905Chembridge (Drug-like library)329.398363.17114cell wall
SGTC_334491488623.9 μM0.3857144905352Chembridge (Drug-like library)349.816843.34914
SGTC_3353915164439.28 μM0.3787884909158Chembridge (Drug-like library)309.408722.87614