9137683

2,8,10-trimethyl-4-piperidin-1-ylpyrido[2,3]pyrazolo[2,4-a]pyrimidine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3268
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 1953228
SMILES CC1=CC(=NC2=NN3C(=CC(=NC3=C12)C)N4CCCCC4)C
Standardized SMILES Cc1cc(C)c2c(n1)nn3c(cc(C)nc23)N4CCCCC4
Molecular weight 295.3821
ALogP 3.15
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.32
% growth inhibition (Hom. pool) -2.59


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1953228
Download HIP data (tab-delimited text)  (excel)
Gene:APC5(YOR249C)|FD-Score:3.64|P-value:1.38E-4|Clearance:0.23||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:DBP5(YOR046C)|FD-Score:-3.46|P-value:2.70E-4|Clearance:0||SGD DESC:Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p Gene:DUT1(YBR252W)|FD-Score:6.33|P-value:1.21E-10|Clearance:1.24||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:ERV1(YGR029W)|FD-Score:3.41|P-value:3.30E-4|Clearance:0.25||SGD DESC:Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) Gene:IQG1(YPL242C)|FD-Score:3.16|P-value:7.91E-4|Clearance:0.14||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RPL33A(YPL143W)|FD-Score:3.72|P-value:1.02E-4|Clearance:0.08||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:RPL5(YPL131W)|FD-Score:4.84|P-value:6.51E-7|Clearance:0.54||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:SEC26(YDR238C)|FD-Score:4.3|P-value:8.39E-6|Clearance:0.59||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:UTP4(YDR324C)|FD-Score:5.09|P-value:1.80E-7|Clearance:0.25||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:APC5(YOR249C)|FD-Score:3.64|P-value:1.38E-4|Clearance:0.23||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:DBP5(YOR046C)|FD-Score:-3.46|P-value:2.70E-4|Clearance:0||SGD DESC:Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p Gene:DUT1(YBR252W)|FD-Score:6.33|P-value:1.21E-10|Clearance:1.24||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:ERV1(YGR029W)|FD-Score:3.41|P-value:3.30E-4|Clearance:0.25||SGD DESC:Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) Gene:IQG1(YPL242C)|FD-Score:3.16|P-value:7.91E-4|Clearance:0.14||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RPL33A(YPL143W)|FD-Score:3.72|P-value:1.02E-4|Clearance:0.08||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:RPL5(YPL131W)|FD-Score:4.84|P-value:6.51E-7|Clearance:0.54||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:SEC26(YDR238C)|FD-Score:4.3|P-value:8.39E-6|Clearance:0.59||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:UTP4(YDR324C)|FD-Score:5.09|P-value:1.80E-7|Clearance:0.25||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1953228
Download HOP data (tab-delimited text)  (excel)
Gene:AIM3(YBR108W)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Protein interacting with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:APP1(YNL094W)|FD-Score:4.02|P-value:2.94E-5||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:ARF2(YDL137W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication Gene:CCS1(YMR038C)|FD-Score:3.73|P-value:9.51E-5||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CPA2(YJR109C)|FD-Score:-3.1|P-value:9.80E-4||SGD DESC:Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor Gene:CPR3(YML078W)|FD-Score:5.19|P-value:1.05E-7||SGD DESC:Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria Gene:ELC1(YPL046C)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair Gene:ELP4(YPL101W)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:EMP47(YFL048C)|FD-Score:-3.9|P-value:4.89E-5||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:FYV7(YLR068W)|FD-Score:-4.02|P-value:2.93E-5||SGD DESC:Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin Gene:GPT2(YKR067W)|FD-Score:4.89|P-value:5.04E-7||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:HST2(YPL015C)|FD-Score:7.14|P-value:4.74E-13||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:IRC20(YLR247C)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Putative helicase; localizes to the mitochondrion and the nucleus; YLR247C is not an essential gene; null mutant displays increased levels of spontaneous Rad52p foci Gene:MDM30(YLR368W)|FD-Score:-3.79|P-value:7.52E-5||SGD DESC:F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains Gene:MET8(YBR213W)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MFG1(YDL233W_p)|FD-Score:-3.13|P-value:8.64E-4||SGD DESC:Regulator of filamentous growth; interacts with FLO11 promoter and regulates FLO11 expression; binds to transcription factors Flo8p and Mss11p; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YDL233W is not an essential gene Gene:MTC6(YHR151C)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:PFK1(YGR240C)|FD-Score:-3.2|P-value:6.99E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PMC1(YGL006W)|FD-Score:3.12|P-value:8.93E-4||SGD DESC:Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium; similar to mammalian PMCA1a Gene:PUF4(YGL014W)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:RAD33(YML011C)|FD-Score:4.39|P-value:5.76E-6||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RMD8(YFR048W)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Cytosolic protein required for sporulation Gene:RPS22B(YLR367W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication Gene:SAS4(YDR181C)|FD-Score:3.11|P-value:9.34E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SUT1(YGL162W)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Transcription factor of the Zn2Cys6 family involved in sterol uptake; involved in induction of hypoxic gene expression; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:VPS69(YPR087W_d)|FD-Score:-3.16|P-value:7.85E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:YBR242W(YBR242W_p)|FD-Score:3.09|P-value:9.92E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YCR050C(YCR050C_p)|FD-Score:4.15|P-value:1.70E-5||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YEF1(YEL041W)|FD-Score:4.45|P-value:4.25E-6||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YER119C-A(YER119C-A_d)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YFR018C(YFR018C_p)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Putative protein of unknown function Gene:YGK3(YOL128C)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YIL025C(YIL025C_d)|FD-Score:3.22|P-value:6.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL028W(YIL028W_d)|FD-Score:4.4|P-value:5.33E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIR035C(YIR035C_p)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Putative cytoplasmic short-chain dehydrogenase/reductase Gene:YPL080C(YPL080C_d)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR170C(YPR170C_d)|FD-Score:3.73|P-value:9.50E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:AIM3(YBR108W)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Protein interacting with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:APP1(YNL094W)|FD-Score:4.02|P-value:2.94E-5||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:ARF2(YDL137W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication Gene:CCS1(YMR038C)|FD-Score:3.73|P-value:9.51E-5||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CPA2(YJR109C)|FD-Score:-3.1|P-value:9.80E-4||SGD DESC:Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor Gene:CPR3(YML078W)|FD-Score:5.19|P-value:1.05E-7||SGD DESC:Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria Gene:ELC1(YPL046C)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair Gene:ELP4(YPL101W)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:EMP47(YFL048C)|FD-Score:-3.9|P-value:4.89E-5||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:FYV7(YLR068W)|FD-Score:-4.02|P-value:2.93E-5||SGD DESC:Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin Gene:GPT2(YKR067W)|FD-Score:4.89|P-value:5.04E-7||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:HST2(YPL015C)|FD-Score:7.14|P-value:4.74E-13||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:IRC20(YLR247C)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Putative helicase; localizes to the mitochondrion and the nucleus; YLR247C is not an essential gene; null mutant displays increased levels of spontaneous Rad52p foci Gene:MDM30(YLR368W)|FD-Score:-3.79|P-value:7.52E-5||SGD DESC:F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains Gene:MET8(YBR213W)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MFG1(YDL233W_p)|FD-Score:-3.13|P-value:8.64E-4||SGD DESC:Regulator of filamentous growth; interacts with FLO11 promoter and regulates FLO11 expression; binds to transcription factors Flo8p and Mss11p; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YDL233W is not an essential gene Gene:MTC6(YHR151C)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:PFK1(YGR240C)|FD-Score:-3.2|P-value:6.99E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PMC1(YGL006W)|FD-Score:3.12|P-value:8.93E-4||SGD DESC:Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium; similar to mammalian PMCA1a Gene:PUF4(YGL014W)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:RAD33(YML011C)|FD-Score:4.39|P-value:5.76E-6||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RMD8(YFR048W)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Cytosolic protein required for sporulation Gene:RPS22B(YLR367W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication Gene:SAS4(YDR181C)|FD-Score:3.11|P-value:9.34E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SUT1(YGL162W)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Transcription factor of the Zn2Cys6 family involved in sterol uptake; involved in induction of hypoxic gene expression; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:VPS69(YPR087W_d)|FD-Score:-3.16|P-value:7.85E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:YBR242W(YBR242W_p)|FD-Score:3.09|P-value:9.92E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YCR050C(YCR050C_p)|FD-Score:4.15|P-value:1.70E-5||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YEF1(YEL041W)|FD-Score:4.45|P-value:4.25E-6||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YER119C-A(YER119C-A_d)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YFR018C(YFR018C_p)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Putative protein of unknown function Gene:YGK3(YOL128C)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YIL025C(YIL025C_d)|FD-Score:3.22|P-value:6.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL028W(YIL028W_d)|FD-Score:4.4|P-value:5.33E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIR035C(YIR035C_p)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Putative cytoplasmic short-chain dehydrogenase/reductase Gene:YPL080C(YPL080C_d)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR170C(YPR170C_d)|FD-Score:3.73|P-value:9.50E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR252W6.331.21E-101.24DUT1deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate
YDR324C5.091.80E-70.25UTP4Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript
YPL131W4.846.51E-70.54RPL5Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly
YDR238C4.308.39E-60.59SEC26Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
YPL143W3.721.02E-40.08RPL33ARibosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication
YOR249C3.641.38E-40.23APC5Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress
YGR029W3.413.30E-40.25ERV1Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR)
YPL242C3.167.91E-40.14IQG1Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress
YGR253C3.020.001250.00PUP2Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
YNL131W3.020.001270.07TOM22Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes
YJL009W_d2.950.001580.01YJL009W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex
YCR035C2.940.001640.09RRP43Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress
YGL123W2.850.002160.01RPS2Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5
YPL160W2.840.002250.00CDC60Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA
YGR265W_d2.840.002260.03YGR265W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL015C7.144.74E-13HST2Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export
YML078W5.191.05E-7CPR3Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria
YKR067W4.895.04E-7GPT2Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen
YEL041W4.454.25E-6YEF1ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication
YIL028W_d4.405.33E-6YIL028W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YML011C4.395.76E-6RAD33Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
YFR018C_p4.211.27E-5YFR018C_pPutative protein of unknown function
YCR050C_p4.151.70E-5YCR050C_pNon-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein
YNL094W4.022.94E-5APP1Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway
YPR170C_d3.739.50E-5YPR170C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B
YMR038C3.739.51E-5CCS1Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress
YGL014W3.611.50E-4PUF4Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors
YLR367W3.581.74E-4RPS22BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication
YHR151C3.551.90E-4MTC6Protein of unknown function; mtc6 is synthetically sick with cdc13-1
YGL162W3.551.95E-4SUT1Transcription factor of the Zn2Cys6 family involved in sterol uptake; involved in induction of hypoxic gene expression; relocalizes from nucleus to cytoplasm upon DNA replication stress

GO enrichment analysis for SGTC_3268
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0912.30E-12SGTC_32739137859 49.5 μMChembridge (Drug-like library)23772340.0769231
0.0672.62E-7SGTC_32659137266 49.5 μMChembridge (Drug-like library)172242650.1
0.0672.79E-7SGTC_32399133757 49.5 μMChembridge (Drug-like library)170369230.0519481
0.0631.22E-6SGTC_28559016525 58.4 μMChembridge (Drug-like library)438114740.115942
0.0603.58E-6SGTC_11750741-0056 27.6 μMChemDiv (Drug-like library)7659270.0526316
0.0571.17E-5SGTC_30509093513 49.5 μMChembridge (Drug-like library)252366640.116883
0.0561.82E-5SGTC_8143342-0569 96.0 μMChemDiv (Drug-like library)43462830.0493827
0.0542.83E-5SGTC_30239089636 49.5 μMChembridge (Drug-like library)172492410.106667
0.0535.34E-5SGTC_31469095609 49.5 μMChembridge (Drug-like library)171739990.118421
0.0526.51E-5SGTC_32639137018 49.5 μMChembridge (Drug-like library)438449570.0897436
0.0519.60E-5SGTC_1459k035-0031 35.7 μMChemDiv (Drug-like library)38771280.05DNA intercalators
0.0511.03E-4SGTC_8053852-0218 479.0 μMChemDiv (Drug-like library)66153550.0705882ERAD & cell cycle
0.0491.56E-4SGTC_1931st074438 57.1 μMTimTec (Natural product derivative library)27873130.0493827
0.0491.58E-4SGTC_21966573537 42.7 μMChembridge (Fragment library)29030870.0806452copper-dependent oxidative stress
0.0491.77E-4SGTC_30519093778 49.5 μMChembridge (Drug-like library)172173780.109589

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3341914722225.87 μM0.51939759Chembridge (Drug-like library)269.344862.6114RNA processing & uracil transport
SGTC_3235913260249.47 μM0.4642864912240Chembridge (Drug-like library)352.430163.31605
SGTC_3328914062429.04 μM0.4181824904392Chembridge (Drug-like library)335.4463.33104
SGTC_3280914096149.47 μM0.389834901226Chembridge (Drug-like library)355.864483.5090460S ribosome export
SGTC_3222912830149.47 μM0.3833334911358Chembridge (Drug-like library)375.390813.9330760S ribosome export
SGTC_3257913920632.98 μM0.3684214910041Chembridge (Drug-like library)321.419423.04804
SGTC_3276913906049.47 μM0.3538464870619Chembridge (Drug-like library)346.428883.6551560S ribosome export
SGTC_3329914123327.56 μM0.3484854909101Chembridge (Drug-like library)405.416793.39418
SGTC_3349914970323 μM0.3333334910494Chembridge (Drug-like library)351.44542.50915
SGTC_3333914182012.09 μM0.3015871939433Chembridge (Drug-like library)349.472583.97404