9137823

4-methyl-N-(3-methylphenyl)-2-pyridin-3-yl-1,3-thiazole-5-carboxamide

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3269
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 17682936
SMILES CC1=CC(=CC=C1)NC(=O)C2=C(N=C(S2)C3=CN=CC=C3)C
Standardized SMILES Cc1cccc(NC(=O)c2sc(nc2C)c3cccnc3)c1
Molecular weight 309.3855
ALogP 2.9
H-bond donor count 1
H-bond acceptor count 4
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.96
% growth inhibition (Hom. pool) 10.2


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17682936
Download HIP data (tab-delimited text)  (excel)
Gene:FMN1(YDR236C)|FD-Score:3.44|P-value:2.94E-4|Clearance:0.03||SGD DESC:Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane Gene:LSG1(YGL099W)|FD-Score:3.41|P-value:3.24E-4|Clearance:0.04||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MTR3(YGR158C)|FD-Score:3.37|P-value:3.77E-4|Clearance:0.41||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NMD3(YHR170W)|FD-Score:5.14|P-value:1.39E-7|Clearance:0.8||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP15(YNL110C)|FD-Score:-4.52|P-value:3.08E-6|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:RAD53(YPL153C)|FD-Score:-3.4|P-value:3.41E-4|Clearance:0||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:REB1(YBR049C)|FD-Score:3.44|P-value:2.91E-4|Clearance:0||SGD DESC:RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication Gene:RRP9(YPR137W)|FD-Score:-3.27|P-value:5.31E-4|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:RSC6(YCR052W)|FD-Score:4.32|P-value:7.89E-6|Clearance:0.36||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC23(YPR181C)|FD-Score:-3.78|P-value:7.97E-5|Clearance:0||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:TSC10(YBR265W)|FD-Score:-3.75|P-value:9.00E-5|Clearance:0||SGD DESC:3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family Gene:YLR230W(YLR230W_d)|FD-Score:4.34|P-value:7.15E-6|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity Gene:YPL142C(YPL142C_d)|FD-Score:3.96|P-value:3.80E-5|Clearance:0.52||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Gene:FMN1(YDR236C)|FD-Score:3.44|P-value:2.94E-4|Clearance:0.03||SGD DESC:Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane Gene:LSG1(YGL099W)|FD-Score:3.41|P-value:3.24E-4|Clearance:0.04||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MTR3(YGR158C)|FD-Score:3.37|P-value:3.77E-4|Clearance:0.41||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NMD3(YHR170W)|FD-Score:5.14|P-value:1.39E-7|Clearance:0.8||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP15(YNL110C)|FD-Score:-4.52|P-value:3.08E-6|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:RAD53(YPL153C)|FD-Score:-3.4|P-value:3.41E-4|Clearance:0||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:REB1(YBR049C)|FD-Score:3.44|P-value:2.91E-4|Clearance:0||SGD DESC:RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication Gene:RRP9(YPR137W)|FD-Score:-3.27|P-value:5.31E-4|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:RSC6(YCR052W)|FD-Score:4.32|P-value:7.89E-6|Clearance:0.36||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC23(YPR181C)|FD-Score:-3.78|P-value:7.97E-5|Clearance:0||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:TSC10(YBR265W)|FD-Score:-3.75|P-value:9.00E-5|Clearance:0||SGD DESC:3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family Gene:YLR230W(YLR230W_d)|FD-Score:4.34|P-value:7.15E-6|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity Gene:YPL142C(YPL142C_d)|FD-Score:3.96|P-value:3.80E-5|Clearance:0.52||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17682936
Download HOP data (tab-delimited text)  (excel)
Gene:AEP3(YPL005W)|FD-Score:4.45|P-value:4.35E-6||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:AIM26(YKL037W)|FD-Score:3.24|P-value:5.87E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:ALG12(YNR030W)|FD-Score:-3.16|P-value:7.95E-4||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:ALG5(YPL227C)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:ATG7(YHR171W)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:ATP3(YBR039W)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:CIT2(YCR005C)|FD-Score:-3.41|P-value:3.21E-4||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, peroxisomal isozyme involved in glyoxylate cycle; expression is controlled by Rtg1p and Rtg2p transcription factors; CIT2 has a paralog, CIT1, that arose from the whole genome duplication Gene:COG5(YNL051W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:3.8|P-value:7.38E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:4.48|P-value:3.74E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPA2(YJR109C)|FD-Score:-3.81|P-value:6.84E-5||SGD DESC:Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor Gene:DAL81(YIR023W)|FD-Score:5.35|P-value:4.46E-8||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DPH1(YIL103W)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p Gene:FAU1(YER183C)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FMP23(YBR047W_p)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GLY1(YEL046C)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:HIT1(YJR055W)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:IDP3(YNL009W)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication Gene:INP51(YIL002C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:IRC6(YFR043C)|FD-Score:3.12|P-value:8.95E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MET17(YLR303W)|FD-Score:-3.86|P-value:5.72E-5||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MFT1(YML062C)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MIR1(YJR077C)|FD-Score:-3.25|P-value:5.78E-4||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated Gene:MRE11(YMR224C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:PAA1(YDR071C)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication Gene:PIR1(YKL164C)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication Gene:PKH2(YOL100W)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PYK2(YOR347C)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication Gene:RAM1(YDL090C)|FD-Score:6.17|P-value:3.31E-10||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RED1(YLR263W)|FD-Score:-3.82|P-value:6.62E-5||SGD DESC:Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p Gene:RGP1(YDR137W)|FD-Score:4.83|P-value:6.80E-7||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RGT1(YKL038W)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:RPL17B(YJL177W)|FD-Score:4.52|P-value:3.11E-6||SGD DESC:Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication Gene:RPL7A(YGL076C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Ribosomal 60S subunit protein L7A; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7A has a paralog, RPL7B, that arose from the whole genome duplication Gene:RPS22A(YJL190C)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RTC2(YBR147W)|FD-Score:-3.33|P-value:4.35E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:SLX9(YGR081C)|FD-Score:-3.48|P-value:2.52E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SPE3(YPR069C)|FD-Score:5.39|P-value:3.49E-8||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SPS2(YDR522C)|FD-Score:-3.1|P-value:9.69E-4||SGD DESC:Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component Gene:SRL1(YOR247W)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:SSA4(YER103W)|FD-Score:4.7|P-value:1.33E-6||SGD DESC:Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication Gene:THP2(YHR167W)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:TKL1(YPR074C)|FD-Score:-3.72|P-value:9.96E-5||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TRP3(YKL211C)|FD-Score:-3.28|P-value:5.24E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TUB3(YML124C)|FD-Score:-5.35|P-value:4.47E-8||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UBC4(YBR082C)|FD-Score:3.19|P-value:7.01E-4||SGD DESC:Ubiquitin-conjugating enzyme (E2); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication Gene:UBC5(YDR059C)|FD-Score:-3.13|P-value:8.69E-4||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication Gene:VPS55(YJR044C)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:VPS63(YLR261C_d)|FD-Score:5.67|P-value:6.99E-9||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YAL044W-A(YAL044W-A_p)|FD-Score:3.88|P-value:5.24E-5||SGD DESC:Putative protein of unknown function; similar to S. pombe uvi31 which is a putative DNA repair protein Gene:YCR102C(YCR102C_p)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YDR433W(YDR433W_d)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER119C-A(YER119C-A_d)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YGL138C(YGL138C_p)|FD-Score:-3.85|P-value:5.88E-5||SGD DESC:Putative protein of unknown function; has no significant sequence similarity to any known protein Gene:YGL188C-A(YGL188C-A_p)|FD-Score:4.66|P-value:1.60E-6||SGD DESC:Putative protein of unknown function Gene:YGR160W(YGR160W_d)|FD-Score:-3.8|P-value:7.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR269W(YGR269W_d)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C Gene:YJR151W-A(YJR151W-A_p)|FD-Score:-3.28|P-value:5.12E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR; predicted to have a role in transcription based on computational guilt by association analysis Gene:YLL056C(YLL056C_p)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Putative protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p and genes involved in pleiotropic drug resistance (PDR); expression is induced in cells treated with the mycotoxin patulin Gene:YLR171W(YLR171W_d)|FD-Score:-4.5|P-value:3.34E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR285C-A(YLR285C-A_p)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YMR105W-A(YMR105W-A_p)|FD-Score:4.33|P-value:7.45E-6||SGD DESC:Putative protein of unknown function Gene:YMR245W(YMR245W_d)|FD-Score:3.74|P-value:9.27E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL324W(YNL324W_d)|FD-Score:3.92|P-value:4.35E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR066C(YNR066C_p)|FD-Score:3.8|P-value:7.15E-5||SGD DESC:Putative membrane-localized protein of unknown function Gene:YOR012W(YOR012W_p)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Putative protein of unknown function Gene:YOR111W(YOR111W_p)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:Putative protein of unknown function Gene:YOR304C-A(YOR304C-A_p)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck Gene:AEP3(YPL005W)|FD-Score:4.45|P-value:4.35E-6||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:AIM26(YKL037W)|FD-Score:3.24|P-value:5.87E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:ALG12(YNR030W)|FD-Score:-3.16|P-value:7.95E-4||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:ALG5(YPL227C)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:ATG7(YHR171W)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:ATP3(YBR039W)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:CIT2(YCR005C)|FD-Score:-3.41|P-value:3.21E-4||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, peroxisomal isozyme involved in glyoxylate cycle; expression is controlled by Rtg1p and Rtg2p transcription factors; CIT2 has a paralog, CIT1, that arose from the whole genome duplication Gene:COG5(YNL051W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:3.8|P-value:7.38E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:4.48|P-value:3.74E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPA2(YJR109C)|FD-Score:-3.81|P-value:6.84E-5||SGD DESC:Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor Gene:DAL81(YIR023W)|FD-Score:5.35|P-value:4.46E-8||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DPH1(YIL103W)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p Gene:FAU1(YER183C)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FMP23(YBR047W_p)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GLY1(YEL046C)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:HIT1(YJR055W)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:IDP3(YNL009W)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication Gene:INP51(YIL002C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:IRC6(YFR043C)|FD-Score:3.12|P-value:8.95E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MET17(YLR303W)|FD-Score:-3.86|P-value:5.72E-5||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MFT1(YML062C)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MIR1(YJR077C)|FD-Score:-3.25|P-value:5.78E-4||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated Gene:MRE11(YMR224C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:PAA1(YDR071C)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication Gene:PIR1(YKL164C)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication Gene:PKH2(YOL100W)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PYK2(YOR347C)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication Gene:RAM1(YDL090C)|FD-Score:6.17|P-value:3.31E-10||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RED1(YLR263W)|FD-Score:-3.82|P-value:6.62E-5||SGD DESC:Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p Gene:RGP1(YDR137W)|FD-Score:4.83|P-value:6.80E-7||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RGT1(YKL038W)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:RPL17B(YJL177W)|FD-Score:4.52|P-value:3.11E-6||SGD DESC:Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication Gene:RPL7A(YGL076C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Ribosomal 60S subunit protein L7A; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7A has a paralog, RPL7B, that arose from the whole genome duplication Gene:RPS22A(YJL190C)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RTC2(YBR147W)|FD-Score:-3.33|P-value:4.35E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:SLX9(YGR081C)|FD-Score:-3.48|P-value:2.52E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SPE3(YPR069C)|FD-Score:5.39|P-value:3.49E-8||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SPS2(YDR522C)|FD-Score:-3.1|P-value:9.69E-4||SGD DESC:Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component Gene:SRL1(YOR247W)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:SSA4(YER103W)|FD-Score:4.7|P-value:1.33E-6||SGD DESC:Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication Gene:THP2(YHR167W)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:TKL1(YPR074C)|FD-Score:-3.72|P-value:9.96E-5||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TRP3(YKL211C)|FD-Score:-3.28|P-value:5.24E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TUB3(YML124C)|FD-Score:-5.35|P-value:4.47E-8||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UBC4(YBR082C)|FD-Score:3.19|P-value:7.01E-4||SGD DESC:Ubiquitin-conjugating enzyme (E2); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication Gene:UBC5(YDR059C)|FD-Score:-3.13|P-value:8.69E-4||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication Gene:VPS55(YJR044C)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:VPS63(YLR261C_d)|FD-Score:5.67|P-value:6.99E-9||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YAL044W-A(YAL044W-A_p)|FD-Score:3.88|P-value:5.24E-5||SGD DESC:Putative protein of unknown function; similar to S. pombe uvi31 which is a putative DNA repair protein Gene:YCR102C(YCR102C_p)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YDR433W(YDR433W_d)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER119C-A(YER119C-A_d)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YGL138C(YGL138C_p)|FD-Score:-3.85|P-value:5.88E-5||SGD DESC:Putative protein of unknown function; has no significant sequence similarity to any known protein Gene:YGL188C-A(YGL188C-A_p)|FD-Score:4.66|P-value:1.60E-6||SGD DESC:Putative protein of unknown function Gene:YGR160W(YGR160W_d)|FD-Score:-3.8|P-value:7.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR269W(YGR269W_d)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C Gene:YJR151W-A(YJR151W-A_p)|FD-Score:-3.28|P-value:5.12E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR; predicted to have a role in transcription based on computational guilt by association analysis Gene:YLL056C(YLL056C_p)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Putative protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p and genes involved in pleiotropic drug resistance (PDR); expression is induced in cells treated with the mycotoxin patulin Gene:YLR171W(YLR171W_d)|FD-Score:-4.5|P-value:3.34E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR285C-A(YLR285C-A_p)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YMR105W-A(YMR105W-A_p)|FD-Score:4.33|P-value:7.45E-6||SGD DESC:Putative protein of unknown function Gene:YMR245W(YMR245W_d)|FD-Score:3.74|P-value:9.27E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL324W(YNL324W_d)|FD-Score:3.92|P-value:4.35E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR066C(YNR066C_p)|FD-Score:3.8|P-value:7.15E-5||SGD DESC:Putative membrane-localized protein of unknown function Gene:YOR012W(YOR012W_p)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Putative protein of unknown function Gene:YOR111W(YOR111W_p)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:Putative protein of unknown function Gene:YOR304C-A(YOR304C-A_p)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR170W5.141.39E-70.80NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YLR230W_d4.347.15E-60.02YLR230W_dDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity
YCR052W4.327.89E-60.36RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YPL142C_d3.963.80E-50.52YPL142C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit
YBR049C3.442.91E-40.00REB1RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication
YDR236C3.442.94E-40.03FMN1Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane
YGL099W3.413.24E-40.04LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YGR158C3.373.77E-40.41MTR3Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
YCR013C_d2.960.001540.08YCR013C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant
YPR113W2.880.001990.02PIS1Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins
YBR087W2.850.002150.02RFC5Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YER112W2.830.002320.24LSM4Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress
YDL102W2.590.004760.05POL3Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)
YMR281W2.540.005490.01GPI12ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp
YKL019W2.540.005580.07RAM2Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL090C6.173.31E-10RAM1Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit
YLR261C_d5.676.99E-9VPS63_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect
YPR069C5.393.49E-8SPE3Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells
YIR023W5.354.46E-8DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YKR019C5.191.03E-7IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YDR137W4.836.80E-7RGP1Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p
YER103W4.701.33E-6SSA4Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication
YGL188C-A_p4.661.60E-6YGL188C-A_pPutative protein of unknown function
YJL177W4.523.11E-6RPL17BRibosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication
YML071C4.483.74E-6COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YPL005W4.454.35E-6AEP3Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA
YMR105W-A_p4.337.45E-6YMR105W-A_pPutative protein of unknown function
YML062C4.241.14E-5MFT1Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance
YDR071C4.151.68E-5PAA1Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication
YHR167W4.111.97E-5THP2Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance

GO enrichment analysis for SGTC_3269
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1722.16E-40SGTC_24605552655 174.2 μMMiscellaneous54019890.157143amide catabolism
0.1262.17E-22SGTC_9903985-0011 41.3 μMChemDiv (Drug-like library)53995650.0921053amide catabolism
0.1255.28E-22SGTC_31689103419 49.5 μMChembridge (Drug-like library)166492560.25352160S ribosome export
0.1226.07E-21SGTC_6051315-0399 119.0 μMChemDiv (Drug-like library)240204610.164557Golgi
0.1219.71E-21SGTC_560957-0365 83.2 μMChemDiv (Drug-like library)67523600.101124Golgi
0.1211.36E-20SGTC_12092323-2347 50.3 μMChemDiv (Drug-like library)54127600.160494
0.1165.55E-19SGTC_486niguldipine 82.0 μMMiscellaneous12360.11amide catabolism
0.1124.60E-18SGTC_10603448-7350 82.0 μMChemDiv (Drug-like library)5701780.153846Golgi
0.1125.16E-18SGTC_5382922-0838 53.3 μMChemDiv (Drug-like library)7770390.147059Golgi
0.1125.17E-18SGTC_14780453-0656 35.7 μMChemDiv (Drug-like library)52584960.15493
0.1128.15E-18SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.148148Golgi
0.1119.24E-18SGTC_624k048-0052 28.0 μMChemDiv (Drug-like library)68355310.16Golgi
0.1119.23E-18SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.067415760S ribosome export
0.1062.50E-16SGTC_1751st044839 27.2 μMTimTec (Natural product derivative library)52913260.1375Golgi
0.1054.62E-16SGTC_31859108267 49.5 μMChembridge (Drug-like library)49104860.075268860S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3337914434233.55 μM0.71153817682949Chembridge (Drug-like library)313.3493832.62415
SGTC_3233913234049.47 μM0.63636417682950Chembridge (Drug-like library)329.803983.08314
SGTC_3215912994249.47 μM0.61403517682944Chembridge (Drug-like library)323.412083.36114
SGTC_3251913504749.47 μM0.61403517682963Chembridge (Drug-like library)325.38492.4021560S ribosome export
SGTC_21505556613200 μM0.509434743190Chembridge (Fragment library)213.235241.22813
SGTC_3229913220449.47 μM0.50769217682924Chembridge (Drug-like library)327.443843.16914
SGTC_8024112-3352742 μM0.465517704594ChemDiv (Drug-like library)267.348823.65914
SGTC_22757947463200 μM0.3965521225414Chembridge (Fragment library)216.235880.85813iron homeostasis
SGTC_2913796655636.57 μM0.3823532976516Chembridge (Drug-like library)372.43813.3982560S ribosome export
SGTC_20134024628200 μM0.3755230760Chembridge (Fragment library)226.273762.35422