9137563

5-amino-1-(2-methylphenyl)-N-phenyltriazole-4-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3270
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 17015724
SMILES CC1=CC=CC=C1N2C(=C(N=N2)C(=O)NC3=CC=CC=C3)N
Standardized SMILES Cc1ccccc1n2nnc(C(=O)Nc3ccccc3)c2N
Molecular weight 293.3232
ALogP 2.67
H-bond donor count 2
H-bond acceptor count 4
Response signature RPP1 & pyrimidine depletion

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.06
% growth inhibition (Hom. pool) 8.18


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17015724
Download HIP data (tab-delimited text)  (excel)
Gene:DPM1(YPR183W)|FD-Score:-3.13|P-value:8.70E-4|Clearance:0||SGD DESC:Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation Gene:DUT1(YBR252W)|FD-Score:-3.83|P-value:6.37E-5|Clearance:0||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:GLC7(YER133W)|FD-Score:6.08|P-value:5.87E-10|Clearance:2.58||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:IQG1(YPL242C)|FD-Score:-4.67|P-value:1.51E-6|Clearance:0||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MCD1(YDL003W)|FD-Score:-3.25|P-value:5.85E-4|Clearance:0||SGD DESC:Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase Gene:NIC96(YFR002W)|FD-Score:-3.41|P-value:3.27E-4|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:RLP24(YLR009W)|FD-Score:3.29|P-value:5.00E-4|Clearance:0.14||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPP1(YHR062C)|FD-Score:3.5|P-value:2.29E-4|Clearance:0.21||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:TFC4(YGR047C)|FD-Score:3.15|P-value:8.04E-4|Clearance:0.08||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102 Gene:TID3(YIL144W)|FD-Score:-3.38|P-value:3.61E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:TSC10(YBR265W)|FD-Score:-3.35|P-value:3.98E-4|Clearance:0||SGD DESC:3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family Gene:YJL195C(YJL195C_d)|FD-Score:-3.57|P-value:1.80E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:DPM1(YPR183W)|FD-Score:-3.13|P-value:8.70E-4|Clearance:0||SGD DESC:Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation Gene:DUT1(YBR252W)|FD-Score:-3.83|P-value:6.37E-5|Clearance:0||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:GLC7(YER133W)|FD-Score:6.08|P-value:5.87E-10|Clearance:2.58||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:IQG1(YPL242C)|FD-Score:-4.67|P-value:1.51E-6|Clearance:0||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MCD1(YDL003W)|FD-Score:-3.25|P-value:5.85E-4|Clearance:0||SGD DESC:Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase Gene:NIC96(YFR002W)|FD-Score:-3.41|P-value:3.27E-4|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:RLP24(YLR009W)|FD-Score:3.29|P-value:5.00E-4|Clearance:0.14||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPP1(YHR062C)|FD-Score:3.5|P-value:2.29E-4|Clearance:0.21||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:TFC4(YGR047C)|FD-Score:3.15|P-value:8.04E-4|Clearance:0.08||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102 Gene:TID3(YIL144W)|FD-Score:-3.38|P-value:3.61E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:TSC10(YBR265W)|FD-Score:-3.35|P-value:3.98E-4|Clearance:0||SGD DESC:3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family Gene:YJL195C(YJL195C_d)|FD-Score:-3.57|P-value:1.80E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17015724
Download HOP data (tab-delimited text)  (excel)
Gene:ATG11(YPR049C)|FD-Score:-4.22|P-value:1.21E-5||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:BMH1(YER177W)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BSD2(YBR290W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Heavy metal ion homeostasis protein, facilitates trafficking of Smf1p and Smf2p metal transporters to the vacuole where they are degraded, controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification Gene:BUD26(YDR241W_d)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CBT1(YKL208W)|FD-Score:-4.29|P-value:9.00E-6||SGD DESC:Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p Gene:CCR4(YAL021C)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CIR2(YOR356W)|FD-Score:3.79|P-value:7.40E-5||SGD DESC:Putative ortholog of human electron transfer flavoprotein dehydrogenase (ETF-dH); found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response Gene:CSM4(YPL200W)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements Gene:CST9(YLR394W)|FD-Score:-4.56|P-value:2.55E-6||SGD DESC:SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate Gene:CUP9(YPL177C)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:DUS4(YLR405W)|FD-Score:-3.52|P-value:2.19E-4||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:ERG4(YGL012W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FAA1(YOR317W)|FD-Score:-3.14|P-value:8.36E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FLD1(YLR404W)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy Gene:FMP30(YPL103C)|FD-Score:-3.39|P-value:3.44E-4||SGD DESC:Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D Gene:FUI1(YBL042C)|FD-Score:4.32|P-value:7.66E-6||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GCN20(YFR009W)|FD-Score:-3.83|P-value:6.46E-5||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GIS3(YLR094C)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Protein of unknown function Gene:HAP5(YOR358W)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex Gene:MET10(YFR030W)|FD-Score:-3.77|P-value:8.31E-5||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MRS3(YJL133W)|FD-Score:4.55|P-value:2.69E-6||SGD DESC:Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication Gene:MSD1(YPL104W)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:NIT2(YJL126W)|FD-Score:4.93|P-value:4.08E-7||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:PGD1(YGL025C)|FD-Score:-3.4|P-value:3.33E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PKR1(YMR123W)|FD-Score:3.74|P-value:9.23E-5||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:POM33(YLL023C)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:Transmembrane nucleoporin; involved in nuclear pore complex (NPC) distribution, assembly or stabilization; highly conserved across species, orthologous to human TMEM33 and paralogous to Per33p; protein abundance increases in response to DNA replication stress Gene:PSR1(YLL010C)|FD-Score:-4.94|P-value:4.01E-7||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:PUS5(YLR165C)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability Gene:PYK2(YOR347C)|FD-Score:-3.26|P-value:5.64E-4||SGD DESC:Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication Gene:RPL24B(YGR148C)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RTC6(YPL183W-A)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SFT2(YBL102W)|FD-Score:4.31|P-value:8.30E-6||SGD DESC:Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 Gene:SGF73(YGL066W)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:UBC11(YOR339C)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Ubiquitin-conjugating enzyme most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2 Gene:XBP1(YIL101C)|FD-Score:-3.38|P-value:3.58E-4||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YDR003W-A(YDR003W-A_p)|FD-Score:5.88|P-value:2.04E-9||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YFL015C(YFL015C_d)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene Gene:YGL138C(YGL138C_p)|FD-Score:-3.83|P-value:6.40E-5||SGD DESC:Putative protein of unknown function; has no significant sequence similarity to any known protein Gene:YGR273C(YGR273C_p)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YOR012W(YOR012W_p)|FD-Score:-4.1|P-value:2.08E-5||SGD DESC:Putative protein of unknown function Gene:YPL080C(YPL080C_d)|FD-Score:-3.52|P-value:2.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL225W(YPL225W)|FD-Score:3.74|P-value:9.26E-5||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPR076W(YPR076W_d)|FD-Score:3.79|P-value:7.63E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YSC84(YHR016C)|FD-Score:-3.1|P-value:9.57E-4||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication Gene:ATG11(YPR049C)|FD-Score:-4.22|P-value:1.21E-5||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:BMH1(YER177W)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BSD2(YBR290W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Heavy metal ion homeostasis protein, facilitates trafficking of Smf1p and Smf2p metal transporters to the vacuole where they are degraded, controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification Gene:BUD26(YDR241W_d)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CBT1(YKL208W)|FD-Score:-4.29|P-value:9.00E-6||SGD DESC:Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p Gene:CCR4(YAL021C)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CIR2(YOR356W)|FD-Score:3.79|P-value:7.40E-5||SGD DESC:Putative ortholog of human electron transfer flavoprotein dehydrogenase (ETF-dH); found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response Gene:CSM4(YPL200W)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements Gene:CST9(YLR394W)|FD-Score:-4.56|P-value:2.55E-6||SGD DESC:SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate Gene:CUP9(YPL177C)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:DUS4(YLR405W)|FD-Score:-3.52|P-value:2.19E-4||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:ERG4(YGL012W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FAA1(YOR317W)|FD-Score:-3.14|P-value:8.36E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FLD1(YLR404W)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy Gene:FMP30(YPL103C)|FD-Score:-3.39|P-value:3.44E-4||SGD DESC:Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D Gene:FUI1(YBL042C)|FD-Score:4.32|P-value:7.66E-6||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GCN20(YFR009W)|FD-Score:-3.83|P-value:6.46E-5||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GIS3(YLR094C)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Protein of unknown function Gene:HAP5(YOR358W)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex Gene:MET10(YFR030W)|FD-Score:-3.77|P-value:8.31E-5||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MRS3(YJL133W)|FD-Score:4.55|P-value:2.69E-6||SGD DESC:Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication Gene:MSD1(YPL104W)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:NIT2(YJL126W)|FD-Score:4.93|P-value:4.08E-7||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:PGD1(YGL025C)|FD-Score:-3.4|P-value:3.33E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PKR1(YMR123W)|FD-Score:3.74|P-value:9.23E-5||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:POM33(YLL023C)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:Transmembrane nucleoporin; involved in nuclear pore complex (NPC) distribution, assembly or stabilization; highly conserved across species, orthologous to human TMEM33 and paralogous to Per33p; protein abundance increases in response to DNA replication stress Gene:PSR1(YLL010C)|FD-Score:-4.94|P-value:4.01E-7||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:PUS5(YLR165C)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability Gene:PYK2(YOR347C)|FD-Score:-3.26|P-value:5.64E-4||SGD DESC:Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication Gene:RPL24B(YGR148C)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RTC6(YPL183W-A)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SFT2(YBL102W)|FD-Score:4.31|P-value:8.30E-6||SGD DESC:Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 Gene:SGF73(YGL066W)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:UBC11(YOR339C)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Ubiquitin-conjugating enzyme most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2 Gene:XBP1(YIL101C)|FD-Score:-3.38|P-value:3.58E-4||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YDR003W-A(YDR003W-A_p)|FD-Score:5.88|P-value:2.04E-9||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YFL015C(YFL015C_d)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene Gene:YGL138C(YGL138C_p)|FD-Score:-3.83|P-value:6.40E-5||SGD DESC:Putative protein of unknown function; has no significant sequence similarity to any known protein Gene:YGR273C(YGR273C_p)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YOR012W(YOR012W_p)|FD-Score:-4.1|P-value:2.08E-5||SGD DESC:Putative protein of unknown function Gene:YPL080C(YPL080C_d)|FD-Score:-3.52|P-value:2.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL225W(YPL225W)|FD-Score:3.74|P-value:9.26E-5||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPR076W(YPR076W_d)|FD-Score:3.79|P-value:7.63E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YSC84(YHR016C)|FD-Score:-3.1|P-value:9.57E-4||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YER133W6.085.87E-102.58GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YHR062C3.502.29E-40.21RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YLR009W3.295.00E-40.14RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YGR047C3.158.04E-40.08TFC4One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102
YLR163C3.070.001060.02MAS1Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
YER025W3.050.001130.06GCD11Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met
YJL090C3.000.001370.02DPB11DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress
YJL002C2.980.001460.12OST1Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
YNL310C2.860.002130.01ZIM17Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix
YKL152C2.850.002170.01GPM1Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis
YGL113W2.840.002260.08SLD3Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress
YGR128C2.760.002870.04UTP8Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YDR045C2.720.003260.01RPC11RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS
YJL011C2.710.003400.03RPC17RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress
YPL190C2.680.003710.01NAB3RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR003W-A_p5.882.04E-9YDR003W-A_pPutative protein of unknown function; identified by expression profiling and mass spectrometry
YJL126W4.934.08E-7NIT2Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member
YJL133W4.552.69E-6MRS3Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication
YBL042C4.327.66E-6FUI1High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress
YBL102W4.318.30E-6SFT2Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5
YDR241W_d4.092.17E-5BUD26_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay
YOR356W3.797.40E-5CIR2Putative ortholog of human electron transfer flavoprotein dehydrogenase (ETF-dH); found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response
YPR076W_d3.797.63E-5YPR076W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YMR123W3.749.23E-5PKR1V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress
YPL225W3.749.26E-5YPL225WProtein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
YGL012W3.571.80E-4ERG4C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
YBR290W3.561.84E-4BSD2Heavy metal ion homeostasis protein, facilitates trafficking of Smf1p and Smf2p metal transporters to the vacuole where they are degraded, controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification
YOR358W3.413.27E-4HAP5Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex
YPL104W3.393.54E-4MSD1Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene
YLR165C3.373.75E-4PUS5Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability

GO enrichment analysis for SGTC_3270
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1141.25E-18SGTC_2743auranofin 4.3 μMMiscellaneous166676690.0533333RPP1 & pyrimidine depletion
0.1001.69E-14SGTC_32729137413 49.5 μMChembridge (Drug-like library)176148700.0895522
0.0952.39E-13SGTC_32619137475 49.5 μMChembridge (Drug-like library)170276900.105263RPP1 & pyrimidine depletion
0.0945.63E-13SGTC_466geldanamycin 89.2 μMICCB bioactive library52883820.0784314RPP1 & pyrimidine depletion
0.0897.49E-12SGTC_2509methyl 7-deshydroxypyrogallin-4-carboxylate 54.6 μMMicrosource (Natural product library)50988840.104478RPP1 & pyrimidine depletion
0.0872.58E-11SGTC_32329132874 49.5 μMChembridge (Drug-like library)37849400.320755RPP1 & pyrimidine depletion
0.0855.28E-11SGTC_1078idebenone 36.1 μMMiscellaneous36860.0625Golgi
0.0841.11E-10SGTC_797k781-0471 270.0 μMChemDiv (Drug-like library)237381290.0972222RPP1 & pyrimidine depletion
0.0831.93E-10SGTC_1625st003711 30.1 μMTimTec (Natural product derivative library)242070340.111111calcium & mitochondrial duress
0.0822.70E-10SGTC_13993562-5772 25.0 μMChemDiv (Drug-like library)238474900.118421RPP1 & pyrimidine depletion
0.0807.10E-10SGTC_2686alizarin 95.4 μMMiscellaneous62930.105263RPP1 & pyrimidine depletion
0.0791.48E-9SGTC_390986-0283 58.3 μMChemDiv (Drug-like library)31106000.258065calcium & mitochondrial duress
0.0772.51E-9SGTC_20575236571 38.3 μMChembridge (Fragment library)54684039NARPP1 & pyrimidine depletion
0.0764.93E-9SGTC_1959st077091 55.8 μMTimTec (Natural product derivative library)8213560.117647RPP1 & pyrimidine depletion
0.0758.16E-9SGTC_120rhodamine 6G 52.3 μMChemDiv (Drug-like library)652110.136364mitochondrial processes

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3355915105844.05 μM0.82222217015728Chembridge (Drug-like library)311.3136632.87125RNA processing & uracil transport
SGTC_3225913081949.47 μM0.6530614547121Chembridge (Drug-like library)293.32322.66624amide catabolism
SGTC_3264913744249.47 μM0.62745117015726Chembridge (Drug-like library)299.370842.94824
SGTC_3274913829849.47 μM0.62517015756Chembridge (Drug-like library)293.32322.66624
SGTC_3228913179949.47 μM0.53703717015818Chembridge (Drug-like library)307.349783.15224amide catabolism
SGTC_3245913482149.47 μM0.517014901Chembridge (Drug-like library)307.349783.12224
SGTC_3168910341949.47 μM0.48214316649256Chembridge (Drug-like library)308.334542.9311460S ribosome export
SGTC_3201911399949.47 μM0.46428616649224Chembridge (Drug-like library)336.38773.90314
SGTC_3095911720749.47 μM0.44067817015229Chembridge (Drug-like library)344.366644.49314
SGTC_3079911751249.47 μM0.4310347185044Chembridge (Drug-like library)338.360522.91515