9137798

5-amino-N-cyclohexyl-1-(3-methylphenyl)triazole-4-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3271
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 6498326
SMILES CC1=CC(=CC=C1)N2C(=C(N=N2)C(=O)NC3CCCCC3)N
Standardized SMILES Cc1cccc(c1)n2nnc(C(=O)NC3CCCCC3)c2N
Molecular weight 299.3708
ALogP 2.95
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.07
% growth inhibition (Hom. pool) 8.18


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6498326
Download HIP data (tab-delimited text)  (excel)
Gene:CWC2(YDL209C)|FD-Score:-5.02|P-value:2.56E-7|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:GCD1(YOR260W)|FD-Score:3.14|P-value:8.32E-4|Clearance:0.03||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:NOP15(YNL110C)|FD-Score:-5.2|P-value:9.93E-8|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:RET1(YOR207C)|FD-Score:6.78|P-value:5.97E-12|Clearance:1.4||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RNA14(YMR061W)|FD-Score:-3.17|P-value:7.58E-4|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPC37(YKR025W)|FD-Score:3.12|P-value:9.11E-4|Clearance:0.03||SGD DESC:RNA polymerase III subunit C37 Gene:RPL17A(YKL180W)|FD-Score:-3.83|P-value:6.48E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SEC23(YPR181C)|FD-Score:3.47|P-value:2.62E-4|Clearance:0.32||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SRP14(YDL092W)|FD-Score:4.11|P-value:2.00E-5|Clearance:0.64||SGD DESC:Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 Gene:YDL152W(YDL152W_d)|FD-Score:5.38|P-value:3.70E-8|Clearance:1.27||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YDL196W(YDL196W_d)|FD-Score:-3.33|P-value:4.40E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:CWC2(YDL209C)|FD-Score:-5.02|P-value:2.56E-7|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:GCD1(YOR260W)|FD-Score:3.14|P-value:8.32E-4|Clearance:0.03||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:NOP15(YNL110C)|FD-Score:-5.2|P-value:9.93E-8|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:RET1(YOR207C)|FD-Score:6.78|P-value:5.97E-12|Clearance:1.4||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RNA14(YMR061W)|FD-Score:-3.17|P-value:7.58E-4|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPC37(YKR025W)|FD-Score:3.12|P-value:9.11E-4|Clearance:0.03||SGD DESC:RNA polymerase III subunit C37 Gene:RPL17A(YKL180W)|FD-Score:-3.83|P-value:6.48E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SEC23(YPR181C)|FD-Score:3.47|P-value:2.62E-4|Clearance:0.32||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SRP14(YDL092W)|FD-Score:4.11|P-value:2.00E-5|Clearance:0.64||SGD DESC:Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 Gene:YDL152W(YDL152W_d)|FD-Score:5.38|P-value:3.70E-8|Clearance:1.27||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YDL196W(YDL196W_d)|FD-Score:-3.33|P-value:4.40E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6498326
Download HOP data (tab-delimited text)  (excel)
Gene:ADD66(YKL206C)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly Gene:ATG32(YIL146C)|FD-Score:-3.99|P-value:3.31E-5||SGD DESC:Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane Gene:AUS1(YOR011W)|FD-Score:3.9|P-value:4.76E-5||SGD DESC:Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen Gene:CAR1(YPL111W)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Arginase, responsible for arginine degradation, expression responds to both induction by arginine and nitrogen catabolite repression; disruption enhances freeze tolerance Gene:CRC1(YOR100C)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:Mitochondrial inner membrane carnitine transporter, required for carnitine-dependent transport of acetyl-CoA from peroxisomes to mitochondria during fatty acid beta-oxidation Gene:ECM23(YPL021W)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p Gene:EFT2(YDR385W)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication Gene:GIM5(YML094W)|FD-Score:3.19|P-value:7.11E-4||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:IBA57(YJR122W)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:MET10(YFR030W)|FD-Score:-3.17|P-value:7.53E-4||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MET8(YBR213W)|FD-Score:-3.11|P-value:9.25E-4||SGD DESC:Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MIR1(YJR077C)|FD-Score:-3.15|P-value:8.12E-4||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated Gene:NIT2(YJL126W)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:NMA111(YNL123W)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:OPI7(YDR360W_d)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. Gene:PAF1(YBR279W)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PIR1(YKL164C)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication Gene:RBK1(YCR036W)|FD-Score:-3.14|P-value:8.38E-4||SGD DESC:Putative ribokinase Gene:RPS22B(YLR367W)|FD-Score:-3.19|P-value:7.16E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication Gene:SDC25(YLL016W)|FD-Score:-3.72|P-value:9.86E-5||SGD DESC:Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL017W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication Gene:SEC66(YBR171W)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SEM1(YDR363W-A)|FD-Score:-3.85|P-value:5.95E-5||SGD DESC:Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress Gene:SKI7(YOR076C)|FD-Score:4.54|P-value:2.76E-6||SGD DESC:Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype Gene:SPO20(YMR017W)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:Meiosis-specific subunit of the t-SNARE complex, required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog Gene:SPT3(YDR392W)|FD-Score:3.81|P-value:6.92E-5||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SUR4(YLR372W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TAT2(YOL020W)|FD-Score:4.29|P-value:9.09E-6||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:UBC4(YBR082C)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Ubiquitin-conjugating enzyme (E2); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication Gene:YBT1(YLL048C)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YDR048C(YDR048C_d)|FD-Score:-3.34|P-value:4.16E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFR018C(YFR018C_p)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Putative protein of unknown function Gene:YGR127W(YGR127W_p)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response Gene:YHR177W(YHR177W_p)|FD-Score:-4.12|P-value:1.90E-5||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YLL056C(YLL056C_p)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Putative protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p and genes involved in pleiotropic drug resistance (PDR); expression is induced in cells treated with the mycotoxin patulin Gene:YLL058W(YLL058W_p)|FD-Score:4.58|P-value:2.32E-6||SGD DESC:Putative protein of unknown function with similarity to Str2p, which is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene Gene:YLR184W(YLR184W_d)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR236C(YLR236C_d)|FD-Score:-4.03|P-value:2.81E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR247W-A(YMR247W-A_p)|FD-Score:-3.92|P-value:4.35E-5||SGD DESC:Putative protein of unknown function Gene:YNL095C(YNL095C_p)|FD-Score:3.89|P-value:4.93E-5||SGD DESC:Putative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication Gene:ADD66(YKL206C)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly Gene:ATG32(YIL146C)|FD-Score:-3.99|P-value:3.31E-5||SGD DESC:Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane Gene:AUS1(YOR011W)|FD-Score:3.9|P-value:4.76E-5||SGD DESC:Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen Gene:CAR1(YPL111W)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Arginase, responsible for arginine degradation, expression responds to both induction by arginine and nitrogen catabolite repression; disruption enhances freeze tolerance Gene:CRC1(YOR100C)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:Mitochondrial inner membrane carnitine transporter, required for carnitine-dependent transport of acetyl-CoA from peroxisomes to mitochondria during fatty acid beta-oxidation Gene:ECM23(YPL021W)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p Gene:EFT2(YDR385W)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication Gene:GIM5(YML094W)|FD-Score:3.19|P-value:7.11E-4||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:IBA57(YJR122W)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:MET10(YFR030W)|FD-Score:-3.17|P-value:7.53E-4||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MET8(YBR213W)|FD-Score:-3.11|P-value:9.25E-4||SGD DESC:Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MIR1(YJR077C)|FD-Score:-3.15|P-value:8.12E-4||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated Gene:NIT2(YJL126W)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:NMA111(YNL123W)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:OPI7(YDR360W_d)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. Gene:PAF1(YBR279W)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PIR1(YKL164C)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication Gene:RBK1(YCR036W)|FD-Score:-3.14|P-value:8.38E-4||SGD DESC:Putative ribokinase Gene:RPS22B(YLR367W)|FD-Score:-3.19|P-value:7.16E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication Gene:SDC25(YLL016W)|FD-Score:-3.72|P-value:9.86E-5||SGD DESC:Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL017W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication Gene:SEC66(YBR171W)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SEM1(YDR363W-A)|FD-Score:-3.85|P-value:5.95E-5||SGD DESC:Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress Gene:SKI7(YOR076C)|FD-Score:4.54|P-value:2.76E-6||SGD DESC:Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype Gene:SPO20(YMR017W)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:Meiosis-specific subunit of the t-SNARE complex, required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog Gene:SPT3(YDR392W)|FD-Score:3.81|P-value:6.92E-5||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SUR4(YLR372W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TAT2(YOL020W)|FD-Score:4.29|P-value:9.09E-6||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:UBC4(YBR082C)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Ubiquitin-conjugating enzyme (E2); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication Gene:YBT1(YLL048C)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YDR048C(YDR048C_d)|FD-Score:-3.34|P-value:4.16E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFR018C(YFR018C_p)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Putative protein of unknown function Gene:YGR127W(YGR127W_p)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response Gene:YHR177W(YHR177W_p)|FD-Score:-4.12|P-value:1.90E-5||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YLL056C(YLL056C_p)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Putative protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p and genes involved in pleiotropic drug resistance (PDR); expression is induced in cells treated with the mycotoxin patulin Gene:YLL058W(YLL058W_p)|FD-Score:4.58|P-value:2.32E-6||SGD DESC:Putative protein of unknown function with similarity to Str2p, which is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene Gene:YLR184W(YLR184W_d)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR236C(YLR236C_d)|FD-Score:-4.03|P-value:2.81E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR247W-A(YMR247W-A_p)|FD-Score:-3.92|P-value:4.35E-5||SGD DESC:Putative protein of unknown function Gene:YNL095C(YNL095C_p)|FD-Score:3.89|P-value:4.93E-5||SGD DESC:Putative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR207C6.785.97E-121.40RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YDL152W_d5.383.70E-81.27YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YDL092W4.112.00E-50.64SRP14Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14
YPR181C3.472.62E-40.32SEC23GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p
YOR260W3.148.32E-40.03GCD1Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YKR025W3.129.11E-40.03RPC37RNA polymerase III subunit C37
YDL150W3.090.001010.02RPC53RNA polymerase III subunit C53
YDR527W3.070.001070.03RBA50Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
YOR319W3.040.001180.19HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YFL024C2.850.002160.03EPL1Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb
YPL007C2.830.002360.02TFC8One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90
YER127W2.800.002530.02LCP5Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus
YGL044C2.780.002680.06RNA15Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YDL097C2.730.003180.03RPN6Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress
YDR434W2.700.003450.05GPI17Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLL058W_p4.582.32E-6YLL058W_pPutative protein of unknown function with similarity to Str2p, which is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene
YOR076C4.542.76E-6SKI7Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype
YOL020W4.299.09E-6TAT2High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance
YPL021W3.993.30E-5ECM23Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p
YOR011W3.904.76E-5AUS1Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen
YNL095C_p3.894.93E-5YNL095C_pPutative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication
YKL206C3.885.16E-5ADD66Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly
YDR392W3.816.92E-5SPT3Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
YLR372W3.701.07E-4SUR4Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
YLL056C_p3.611.51E-4YLL056C_pPutative protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p and genes involved in pleiotropic drug resistance (PDR); expression is induced in cells treated with the mycotoxin patulin
YBR171W3.442.91E-4SEC66Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YGR127W_p3.413.22E-4YGR127W_pPutative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response
YDR385W3.403.31E-4EFT2Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication
YBR082C3.403.39E-4UBC4Ubiquitin-conjugating enzyme (E2); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication
YBR279W3.334.37E-4PAF1Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1

GO enrichment analysis for SGTC_3271
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1433.13E-28SGTC_2522biochanin a 4.9 μMMicrosource (Natural product library)52803730.0769231RNA pol III & RNase P/MRP
0.1343.48E-25SGTC_1915st067105 30.6 μMTimTec (Natural product derivative library)697510.1RNA pol III & RNase P/MRP
0.1232.20E-21SGTC_2738aclarubicin 5.5 μMMiscellaneous4514150.104839RNA pol III & RNase P/MRP
0.1132.38E-18SGTC_2411st077133 39.9 μMTimTec (Natural product derivative library)14031420.0987654RNA pol III & RNase P/MRP
0.1101.85E-17SGTC_29329016711 44.5 μMChembridge (Drug-like library)71173280.113924
0.1057.17E-16SGTC_503tyrphostin 8 294.0 μMICCB bioactive library929700.0625TSC3-RPN4
0.1017.28E-15SGTC_2544sphondin 68.5 μMMicrosource (Natural product library)1081040.08RNA pol III & RNase P/MRP
0.0985.07E-14SGTC_1643st009866 20.2 μMTimTec (Natural product derivative library)31562730.120482
0.0971.01E-13SGTC_2103551-0445 56.8 μMChemDiv (Drug-like library)28651800.117647
0.0936.36E-13SGTC_32649137442 49.5 μMChembridge (Drug-like library)170157260.666667
0.0898.36E-12SGTC_32619137475 49.5 μMChembridge (Drug-like library)170276900.1375RPP1 & pyrimidine depletion
0.0881.54E-11SGTC_2612dictamnine 15.5 μMMicrosource (Natural product library)680850.0857143RNA pol III & RNase P/MRP
0.0822.72E-10SGTC_33559151058 44.0 μMChembridge (Drug-like library)170157280.369231RNA processing & uracil transport
0.0815.40E-10SGTC_2510benzyl isothiocyanate 8.3 μMMicrosource (Natural product library)23460.0645161
0.0809.10E-10SGTC_2233sulbentine 40.4 μMMiscellaneous676860.0704225

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3212912830749.47 μM0.7527243542Chembridge (Drug-like library)350.213742.75424
SGTC_3264913744249.47 μM0.66666717015726Chembridge (Drug-like library)299.370842.94824
SGTC_3225913081949.47 μM0.629634547121Chembridge (Drug-like library)293.32322.66624amide catabolism
SGTC_3228913179949.47 μM0.60714317015818Chembridge (Drug-like library)307.349783.15224amide catabolism
SGTC_3274913829849.47 μM0.46551717015756Chembridge (Drug-like library)293.32322.66624
SGTC_3220913126949.47 μM0.437517722206Chembridge (Drug-like library)377.439623.68324
SGTC_3263913701849.47 μM0.42424243844957Chembridge (Drug-like library)336.38773.20624
SGTC_3245913482149.47 μM0.41935517014901Chembridge (Drug-like library)307.349783.12224
SGTC_3270913756349.47 μM0.39344317015724Chembridge (Drug-like library)293.32322.66624RPP1 & pyrimidine depletion
SGTC_3355915105844.05 μM0.36923117015728Chembridge (Drug-like library)311.3136632.87125RNA processing & uracil transport