9138298

5-amino-N-(2-methylphenyl)-1-phenyltriazole-4-carboxamide

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3274
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 17015756
SMILES CC1=CC=CC=C1NC(=O)C2=C(N(N=N2)C3=CC=CC=C3)N
Standardized SMILES Cc1ccccc1NC(=O)c2nnn(c2N)c3ccccc3
Molecular weight 293.3232
ALogP 2.67
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.96
% growth inhibition (Hom. pool) 9.19


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17015756
Download HIP data (tab-delimited text)  (excel)
Gene:BRF1(YGR246C)|FD-Score:3.22|P-value:6.44E-4|Clearance:0.12||SGD DESC:TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB Gene:CFT1(YDR301W)|FD-Score:3.74|P-value:9.05E-5|Clearance:0.2||SGD DESC:RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF Gene:ECM9(YKR004C)|FD-Score:3.44|P-value:2.95E-4|Clearance:0.08||SGD DESC:Non-essential protein of unknown function Gene:MAK11(YKL021C)|FD-Score:3.54|P-value:1.99E-4|Clearance:0.06||SGD DESC:Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats Gene:NIC96(YFR002W)|FD-Score:3.36|P-value:3.88E-4|Clearance:0.14||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:PIS1(YPR113W)|FD-Score:-3.46|P-value:2.66E-4|Clearance:0||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:RPA43(YOR340C)|FD-Score:3.1|P-value:9.74E-4|Clearance:0.08||SGD DESC:RNA polymerase I subunit A43 Gene:RSC58(YLR033W)|FD-Score:4.06|P-value:2.47E-5|Clearance:0.1||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SEN1(YLR430W)|FD-Score:3.48|P-value:2.49E-4|Clearance:0.05||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:TAF6(YGL112C)|FD-Score:6.7|P-value:1.05E-11|Clearance:1.23||SGD DESC:Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 Gene:TSR1(YDL060W)|FD-Score:4.87|P-value:5.57E-7|Clearance:0.81||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:YDL221W(YDL221W_d)|FD-Score:-3.24|P-value:5.92E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13 Gene:YJL009W(YJL009W_d)|FD-Score:3.96|P-value:3.82E-5|Clearance:0.21||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YJL202C(YJL202C_d)|FD-Score:5.47|P-value:2.31E-8|Clearance:0.59||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex Gene:BRF1(YGR246C)|FD-Score:3.22|P-value:6.44E-4|Clearance:0.12||SGD DESC:TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB Gene:CFT1(YDR301W)|FD-Score:3.74|P-value:9.05E-5|Clearance:0.2||SGD DESC:RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF Gene:ECM9(YKR004C)|FD-Score:3.44|P-value:2.95E-4|Clearance:0.08||SGD DESC:Non-essential protein of unknown function Gene:MAK11(YKL021C)|FD-Score:3.54|P-value:1.99E-4|Clearance:0.06||SGD DESC:Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats Gene:NIC96(YFR002W)|FD-Score:3.36|P-value:3.88E-4|Clearance:0.14||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:PIS1(YPR113W)|FD-Score:-3.46|P-value:2.66E-4|Clearance:0||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:RPA43(YOR340C)|FD-Score:3.1|P-value:9.74E-4|Clearance:0.08||SGD DESC:RNA polymerase I subunit A43 Gene:RSC58(YLR033W)|FD-Score:4.06|P-value:2.47E-5|Clearance:0.1||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SEN1(YLR430W)|FD-Score:3.48|P-value:2.49E-4|Clearance:0.05||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:TAF6(YGL112C)|FD-Score:6.7|P-value:1.05E-11|Clearance:1.23||SGD DESC:Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 Gene:TSR1(YDL060W)|FD-Score:4.87|P-value:5.57E-7|Clearance:0.81||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:YDL221W(YDL221W_d)|FD-Score:-3.24|P-value:5.92E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13 Gene:YJL009W(YJL009W_d)|FD-Score:3.96|P-value:3.82E-5|Clearance:0.21||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YJL202C(YJL202C_d)|FD-Score:5.47|P-value:2.31E-8|Clearance:0.59||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17015756
Download HOP data (tab-delimited text)  (excel)
Gene:ADO1(YJR105W)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AIM14(YGL160W)|FD-Score:-3.38|P-value:3.59E-4||SGD DESC:NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p Gene:AIM20(YIL158W)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:AIM4(YBR194W)|FD-Score:4.28|P-value:9.51E-6||SGD DESC:Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress Gene:ARC18(YLR370C)|FD-Score:6.11|P-value:4.84E-10||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ATG18(YFR021W)|FD-Score:-4.65|P-value:1.70E-6||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:BNA2(YJR078W)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Putative tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase, required for de novo biosynthesis of NAD from tryptophan via kynurenine; interacts genetically with telomere capping gene CDC13; regulated by Hst1p and Aftp Gene:CDC73(YLR418C)|FD-Score:4.58|P-value:2.32E-6||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CEX1(YOR112W)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:COQ3(YOL096C)|FD-Score:3.75|P-value:8.78E-5||SGD DESC:O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein Gene:DAL81(YIR023W)|FD-Score:4.61|P-value:2.06E-6||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DCS1(YLR270W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DFM1(YDR411C)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p Gene:DIG1(YPL049C)|FD-Score:3.1|P-value:9.64E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DTR1(YBR180W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation Gene:EAF1(YDR359C)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 Gene:ELC1(YPL046C)|FD-Score:-3.25|P-value:5.80E-4||SGD DESC:Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair Gene:EMP47(YFL048C)|FD-Score:-3.98|P-value:3.43E-5||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:EPS1(YIL005W)|FD-Score:-4.01|P-value:3.07E-5||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:FPR2(YDR519W)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress Gene:GAS1(YMR307W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GND1(YHR183W)|FD-Score:-3.56|P-value:1.89E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:GZF3(YJL110C)|FD-Score:-3.23|P-value:6.12E-4||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HCM1(YCR065W)|FD-Score:-3.22|P-value:6.31E-4||SGD DESC:Forkhead transcription factor that drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role Gene:HOC1(YJR075W)|FD-Score:-3.12|P-value:8.94E-4||SGD DESC:Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele Gene:IDH2(YOR136W)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IOC4(YMR044W)|FD-Score:4.27|P-value:9.89E-6||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:IST2(YBR086C)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process Gene:JIP4(YDR475C)|FD-Score:-4.59|P-value:2.21E-6||SGD DESC:Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence Gene:KTR1(YOR099W)|FD-Score:-4.55|P-value:2.67E-6||SGD DESC:Alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; type II membrane protein; member of the KRE2/MNT1 mannosyltransferase family; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:MAC1(YMR021C)|FD-Score:4.51|P-value:3.26E-6||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MCX1(YBR227C)|FD-Score:-3.62|P-value:1.50E-4||SGD DESC:Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins Gene:MMM1(YLL006W)|FD-Score:4.59|P-value:2.19E-6||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRPL33(YMR286W)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS28(YDR337W)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPS5(YBR251W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NCS6(YGL211W)|FD-Score:3.88|P-value:5.18E-5||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NUP120(YKL057C)|FD-Score:5.26|P-value:7.32E-8||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:NUP133(YKR082W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP133 Gene:OXR1(YPL196W)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes Gene:PEX1(YKL197C)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis Gene:PSR1(YLL010C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:PYK2(YOR347C)|FD-Score:-4.6|P-value:2.12E-6||SGD DESC:Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication Gene:REX2(YLR059C)|FD-Score:3.84|P-value:6.10E-5||SGD DESC:3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease Gene:RRT7(YLL030C_d)|FD-Score:4.95|P-value:3.68E-7||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:SFT2(YBL102W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 Gene:SHY1(YGR112W)|FD-Score:-3.11|P-value:9.22E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; similar to human SURF1 involved in Leigh Syndrome Gene:STP1(YDR463W)|FD-Score:4.38|P-value:6.00E-6||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:SWE1(YJL187C)|FD-Score:-4.05|P-value:2.51E-5||SGD DESC:Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate Gene:TGL2(YDR058C)|FD-Score:3.42|P-value:3.19E-4||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TUB3(YML124C)|FD-Score:-4.92|P-value:4.26E-7||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UBP11(YKR098C)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins Gene:VAM3(YOR106W)|FD-Score:5.32|P-value:5.06E-8||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VBA2(YBR293W)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VEL1(YGL258W)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VMA13(YPR036W)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS21(YOR089C)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS70(YJR126C)|FD-Score:-3.38|P-value:3.68E-4||SGD DESC:Protein of unknown function involved in vacuolar protein sorting Gene:YAL037W(YAL037W_p)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication Gene:YDL162C(YDL162C_d)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly Gene:YDR008C(YDR008C_d)|FD-Score:-6.63|P-value:1.69E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER137C(YER137C_p)|FD-Score:4.47|P-value:3.90E-6||SGD DESC:Putative protein of unknown function Gene:YGR242W(YGR242W_d)|FD-Score:4.01|P-value:3.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C Gene:YHR007C-A(YHR007C-A_p)|FD-Score:-3.82|P-value:6.54E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YHR177W(YHR177W_p)|FD-Score:7.73|P-value:5.43E-15||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YIL029C(YIL029C_p)|FD-Score:4.06|P-value:2.42E-5||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YIL054W(YIL054W_p)|FD-Score:4.7|P-value:1.30E-6||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YLL053C(YLL053C_p)|FD-Score:-3.38|P-value:3.59E-4||SGD DESC:Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin Gene:YLR225C(YLR225C_p)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene Gene:YLR460C(YLR460C_p)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YMR160W(YMR160W_p)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress Gene:YMR247W-A(YMR247W-A_p)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Putative protein of unknown function Gene:YMR310C(YMR310C_p)|FD-Score:-3.28|P-value:5.19E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene Gene:YNG1(YOR064C)|FD-Score:-3.37|P-value:3.77E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1 Gene:YOL019W(YOL019W_p)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication Gene:YOR343C(YOR343C_d)|FD-Score:-3.73|P-value:9.56E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL080C(YPL080C_d)|FD-Score:-4.36|P-value:6.39E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL199C(YPL199C_p)|FD-Score:3.81|P-value:6.86E-5||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated Gene:YPR197C(YPR197C_d)|FD-Score:4.87|P-value:5.55E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ZEO1(YOL109W)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria Gene:ADO1(YJR105W)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AIM14(YGL160W)|FD-Score:-3.38|P-value:3.59E-4||SGD DESC:NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p Gene:AIM20(YIL158W)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:AIM4(YBR194W)|FD-Score:4.28|P-value:9.51E-6||SGD DESC:Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress Gene:ARC18(YLR370C)|FD-Score:6.11|P-value:4.84E-10||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ATG18(YFR021W)|FD-Score:-4.65|P-value:1.70E-6||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:BNA2(YJR078W)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Putative tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase, required for de novo biosynthesis of NAD from tryptophan via kynurenine; interacts genetically with telomere capping gene CDC13; regulated by Hst1p and Aftp Gene:CDC73(YLR418C)|FD-Score:4.58|P-value:2.32E-6||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CEX1(YOR112W)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:COQ3(YOL096C)|FD-Score:3.75|P-value:8.78E-5||SGD DESC:O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein Gene:DAL81(YIR023W)|FD-Score:4.61|P-value:2.06E-6||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DCS1(YLR270W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DFM1(YDR411C)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p Gene:DIG1(YPL049C)|FD-Score:3.1|P-value:9.64E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DTR1(YBR180W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation Gene:EAF1(YDR359C)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 Gene:ELC1(YPL046C)|FD-Score:-3.25|P-value:5.80E-4||SGD DESC:Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair Gene:EMP47(YFL048C)|FD-Score:-3.98|P-value:3.43E-5||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:EPS1(YIL005W)|FD-Score:-4.01|P-value:3.07E-5||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:FPR2(YDR519W)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress Gene:GAS1(YMR307W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GND1(YHR183W)|FD-Score:-3.56|P-value:1.89E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:GZF3(YJL110C)|FD-Score:-3.23|P-value:6.12E-4||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HCM1(YCR065W)|FD-Score:-3.22|P-value:6.31E-4||SGD DESC:Forkhead transcription factor that drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role Gene:HOC1(YJR075W)|FD-Score:-3.12|P-value:8.94E-4||SGD DESC:Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele Gene:IDH2(YOR136W)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IOC4(YMR044W)|FD-Score:4.27|P-value:9.89E-6||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:IST2(YBR086C)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process Gene:JIP4(YDR475C)|FD-Score:-4.59|P-value:2.21E-6||SGD DESC:Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence Gene:KTR1(YOR099W)|FD-Score:-4.55|P-value:2.67E-6||SGD DESC:Alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; type II membrane protein; member of the KRE2/MNT1 mannosyltransferase family; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:MAC1(YMR021C)|FD-Score:4.51|P-value:3.26E-6||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MCX1(YBR227C)|FD-Score:-3.62|P-value:1.50E-4||SGD DESC:Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins Gene:MMM1(YLL006W)|FD-Score:4.59|P-value:2.19E-6||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRPL33(YMR286W)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS28(YDR337W)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPS5(YBR251W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NCS6(YGL211W)|FD-Score:3.88|P-value:5.18E-5||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NUP120(YKL057C)|FD-Score:5.26|P-value:7.32E-8||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:NUP133(YKR082W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP133 Gene:OXR1(YPL196W)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes Gene:PEX1(YKL197C)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis Gene:PSR1(YLL010C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:PYK2(YOR347C)|FD-Score:-4.6|P-value:2.12E-6||SGD DESC:Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication Gene:REX2(YLR059C)|FD-Score:3.84|P-value:6.10E-5||SGD DESC:3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease Gene:RRT7(YLL030C_d)|FD-Score:4.95|P-value:3.68E-7||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:SFT2(YBL102W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 Gene:SHY1(YGR112W)|FD-Score:-3.11|P-value:9.22E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; similar to human SURF1 involved in Leigh Syndrome Gene:STP1(YDR463W)|FD-Score:4.38|P-value:6.00E-6||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:SWE1(YJL187C)|FD-Score:-4.05|P-value:2.51E-5||SGD DESC:Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate Gene:TGL2(YDR058C)|FD-Score:3.42|P-value:3.19E-4||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TUB3(YML124C)|FD-Score:-4.92|P-value:4.26E-7||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UBP11(YKR098C)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins Gene:VAM3(YOR106W)|FD-Score:5.32|P-value:5.06E-8||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VBA2(YBR293W)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VEL1(YGL258W)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VMA13(YPR036W)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS21(YOR089C)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS70(YJR126C)|FD-Score:-3.38|P-value:3.68E-4||SGD DESC:Protein of unknown function involved in vacuolar protein sorting Gene:YAL037W(YAL037W_p)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication Gene:YDL162C(YDL162C_d)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly Gene:YDR008C(YDR008C_d)|FD-Score:-6.63|P-value:1.69E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER137C(YER137C_p)|FD-Score:4.47|P-value:3.90E-6||SGD DESC:Putative protein of unknown function Gene:YGR242W(YGR242W_d)|FD-Score:4.01|P-value:3.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C Gene:YHR007C-A(YHR007C-A_p)|FD-Score:-3.82|P-value:6.54E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YHR177W(YHR177W_p)|FD-Score:7.73|P-value:5.43E-15||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YIL029C(YIL029C_p)|FD-Score:4.06|P-value:2.42E-5||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YIL054W(YIL054W_p)|FD-Score:4.7|P-value:1.30E-6||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YLL053C(YLL053C_p)|FD-Score:-3.38|P-value:3.59E-4||SGD DESC:Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin Gene:YLR225C(YLR225C_p)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene Gene:YLR460C(YLR460C_p)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YMR160W(YMR160W_p)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress Gene:YMR247W-A(YMR247W-A_p)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Putative protein of unknown function Gene:YMR310C(YMR310C_p)|FD-Score:-3.28|P-value:5.19E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene Gene:YNG1(YOR064C)|FD-Score:-3.37|P-value:3.77E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1 Gene:YOL019W(YOL019W_p)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication Gene:YOR343C(YOR343C_d)|FD-Score:-3.73|P-value:9.56E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL080C(YPL080C_d)|FD-Score:-4.36|P-value:6.39E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL199C(YPL199C_p)|FD-Score:3.81|P-value:6.86E-5||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated Gene:YPR197C(YPR197C_d)|FD-Score:4.87|P-value:5.55E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ZEO1(YOL109W)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL112C6.701.05E-111.23TAF6Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4
YJL202C_d5.472.31E-80.59YJL202C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex
YDL060W4.875.57E-70.81TSR1Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
YLR033W4.062.47E-50.10RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YJL009W_d3.963.82E-50.21YJL009W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex
YDR301W3.749.05E-50.20CFT1RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF
YKL021C3.541.99E-40.06MAK11Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats
YLR430W3.482.49E-40.05SEN1Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS
YKR004C3.442.95E-40.08ECM9Non-essential protein of unknown function
YFR002W3.363.88E-40.14NIC96Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p)
YGR246C3.226.44E-40.12BRF1TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB
YOR340C3.109.74E-40.08RPA43RNA polymerase I subunit A43
YKL144C3.020.001280.02RPC25RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p
YDR062W3.000.001360.00LCB2Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
YPL044C_d3.000.001370.04YPL044C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR177W_p7.735.43E-15YHR177W_pPutative protein of unknown function; overexpression causes a cell cycle delay or arrest
YLR370C6.114.84E-10ARC18Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YOR106W5.325.06E-8VAM3Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region
YKL057C5.267.32E-8NUP120Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160
YOR136W5.151.31E-7IDH2Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated
YLL030C_d4.953.68E-7RRT7_dIdentified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YPR197C_d4.875.55E-7YPR197C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YIL054W_p4.701.30E-6YIL054W_pProtein of unknown function; expressed at both mRNA and protein levels
YIR023W4.612.06E-6DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YLL006W4.592.19E-6MMM1ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth
YLR418C4.582.32E-6CDC73Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress
YMR021C4.513.26E-6MAC1Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport
YER137C_p4.473.90E-6YER137C_pPutative protein of unknown function
YDR463W4.386.00E-6STP1Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication
YBR194W4.289.51E-6AIM4Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress

GO enrichment analysis for SGTC_3274
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1071.47E-16SGTC_24605552655 174.2 μMMiscellaneous54019890.171875amide catabolism
0.0921.75E-12SGTC_33309141424 71.4 μMChembridge (Drug-like library)272610200.118421amide catabolism
0.0897.11E-12SGTC_32289131799 49.5 μMChembridge (Drug-like library)170158180.804348amide catabolism
0.0791.50E-9SGTC_14464239-0190 62.0 μMChemDiv (Drug-like library)13400850.139241
0.0781.60E-9SGTC_253dyclonine 31.2 μMMiscellaneous31800.0985916amide catabolism
0.0758.35E-9SGTC_7003453-2283 142.0 μMChemDiv (Drug-like library)5656640.15625amide catabolism
0.0758.93E-9SGTC_1864st057645 30.4 μMTimTec (Natural product derivative library)6886660.106061amide catabolism
0.0732.09E-8SGTC_22226885208 172.1 μMChembridge (Fragment library)6095140.111111
0.0722.76E-8SGTC_592k241-0073 46.5 μMChemDiv (Drug-like library)42301580.0746269
0.0707.06E-8SGTC_32349132280 49.5 μMChembridge (Drug-like library)176122950.152542
0.0691.27E-7SGTC_458nppb 133.0 μMICCB bioactive library45490.157143
0.0682.08E-7SGTC_30999120367 49.5 μMChembridge (Drug-like library)248249400.114286
0.0672.19E-7SGTC_25792',5'-dihydroxy-4-methoxychalcone 13.2 μMMicrosource (Natural product library)53558880.102941
0.0656.20E-7SGTC_486niguldipine 82.0 μMMiscellaneous12360.117021amide catabolism
0.0631.38E-6SGTC_32729137413 49.5 μMChembridge (Drug-like library)176148700.0895522

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3228913179949.47 μM0.80434817015818Chembridge (Drug-like library)307.349783.15224amide catabolism
SGTC_3245913482149.47 μM0.72340417014901Chembridge (Drug-like library)307.349783.12224
SGTC_3270913756349.47 μM0.62517015724Chembridge (Drug-like library)293.32322.66624RPP1 & pyrimidine depletion
SGTC_3225913081949.47 μM0.624547121Chembridge (Drug-like library)293.32322.66624amide catabolism
SGTC_3355915105844.05 μM0.5471717015728Chembridge (Drug-like library)311.3136632.87125RNA processing & uracil transport
SGTC_3264913744249.47 μM0.48214317015726Chembridge (Drug-like library)299.370842.94824
SGTC_3271913779849.47 μM0.4655176498326Chembridge (Drug-like library)299.370842.94824
SGTC_3079911751249.47 μM0.456147185044Chembridge (Drug-like library)338.360522.91515
SGTC_3192911036249.47 μM0.44262316649480Chembridge (Drug-like library)358.393223.83914mitochondrial processes
SGTC_3095911720749.47 μM0.44067817015229Chembridge (Drug-like library)344.366644.49314