9140961

3-(2-chlorophenyl)-2,5-dimethyl-7-(4-methylpiperazin-1-yl)pyrazolo[1,5-a]pyrimidine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3280
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 4901226
SMILES CC1=NC2=C(C(=NN2C(=C1)N3CCN(CC3)C)C)C4=CC=CC=C4Cl
Standardized SMILES CN1CCN(CC1)c2cc(C)nc3c(c(C)nn23)c4ccccc4Cl
Molecular weight 355.8645
ALogP 3.51
H-bond donor count 0
H-bond acceptor count 4
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.43
% growth inhibition (Hom. pool) 2.25


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4901226
Download HIP data (tab-delimited text)  (excel)
Gene:DUT1(YBR252W)|FD-Score:5.96|P-value:1.26E-9|Clearance:1.5||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:ERG20(YJL167W)|FD-Score:3.44|P-value:2.92E-4|Clearance:0.07||SGD DESC:Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis Gene:GPI8(YDR331W)|FD-Score:3.37|P-value:3.82E-4|Clearance:0.1||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:LSG1(YGL099W)|FD-Score:12.1|P-value:6.22E-34|Clearance:5.43||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NMD3(YHR170W)|FD-Score:6.66|P-value:1.41E-11|Clearance:0.7||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOC4(YPR144C)|FD-Score:-4.53|P-value:2.98E-6|Clearance:0||SGD DESC:Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits Gene:REB1(YBR049C)|FD-Score:3.71|P-value:1.03E-4|Clearance:0.21||SGD DESC:RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication Gene:RIM2(YBR192W)|FD-Score:4.37|P-value:6.14E-6|Clearance:0.09||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RMP1(YLR145W)|FD-Score:4.46|P-value:4.06E-6|Clearance:0.09||SGD DESC:Subunit of RNase MRP, which processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P Gene:RPB10(YOR210W)|FD-Score:-4.88|P-value:5.23E-7|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPS13(YDR064W)|FD-Score:-3.49|P-value:2.37E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:SEC26(YDR238C)|FD-Score:3.5|P-value:2.34E-4|Clearance:0.06||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SEN1(YLR430W)|FD-Score:4.29|P-value:9.05E-6|Clearance:0.58||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:UTP4(YDR324C)|FD-Score:-3.45|P-value:2.84E-4|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YGR073C(YGR073C_d)|FD-Score:3.26|P-value:5.52E-4|Clearance:0.31||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein Gene:YOR146W(YOR146W_d)|FD-Score:-4.07|P-value:2.35E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:YPL238C(YPL238C_d)|FD-Score:-3.18|P-value:7.44E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:DUT1(YBR252W)|FD-Score:5.96|P-value:1.26E-9|Clearance:1.5||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:ERG20(YJL167W)|FD-Score:3.44|P-value:2.92E-4|Clearance:0.07||SGD DESC:Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis Gene:GPI8(YDR331W)|FD-Score:3.37|P-value:3.82E-4|Clearance:0.1||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:LSG1(YGL099W)|FD-Score:12.1|P-value:6.22E-34|Clearance:5.43||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NMD3(YHR170W)|FD-Score:6.66|P-value:1.41E-11|Clearance:0.7||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOC4(YPR144C)|FD-Score:-4.53|P-value:2.98E-6|Clearance:0||SGD DESC:Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits Gene:REB1(YBR049C)|FD-Score:3.71|P-value:1.03E-4|Clearance:0.21||SGD DESC:RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication Gene:RIM2(YBR192W)|FD-Score:4.37|P-value:6.14E-6|Clearance:0.09||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RMP1(YLR145W)|FD-Score:4.46|P-value:4.06E-6|Clearance:0.09||SGD DESC:Subunit of RNase MRP, which processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P Gene:RPB10(YOR210W)|FD-Score:-4.88|P-value:5.23E-7|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPS13(YDR064W)|FD-Score:-3.49|P-value:2.37E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:SEC26(YDR238C)|FD-Score:3.5|P-value:2.34E-4|Clearance:0.06||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SEN1(YLR430W)|FD-Score:4.29|P-value:9.05E-6|Clearance:0.58||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:UTP4(YDR324C)|FD-Score:-3.45|P-value:2.84E-4|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YGR073C(YGR073C_d)|FD-Score:3.26|P-value:5.52E-4|Clearance:0.31||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein Gene:YOR146W(YOR146W_d)|FD-Score:-4.07|P-value:2.35E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:YPL238C(YPL238C_d)|FD-Score:-3.18|P-value:7.44E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4901226
Download HOP data (tab-delimited text)  (excel)
Gene:ADE12(YNL220W)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence Gene:AEP2(YMR282C)|FD-Score:-4.08|P-value:2.25E-5||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:ASM4(YDL088C)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication Gene:AST1(YBL069W)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication Gene:ATG11(YPR049C)|FD-Score:6.71|P-value:9.75E-12||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:ATG17(YLR423C)|FD-Score:3.85|P-value:5.90E-5||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:AXL1(YPR122W)|FD-Score:4.53|P-value:2.93E-6||SGD DESC:Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells Gene:BSC6(YOL137W)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Protein of unknown function containing 8 putative transmembrane seqments; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:COX10(YPL172C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:ECL1(YGR146C)|FD-Score:-4.01|P-value:2.98E-5||SGD DESC:Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:ECM30(YLR436C)|FD-Score:-3.37|P-value:3.72E-4||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm Gene:FMP25(YLR077W)|FD-Score:3.1|P-value:9.64E-4||SGD DESC:Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria Gene:GIP1(YBR045C)|FD-Score:-4.14|P-value:1.71E-5||SGD DESC:Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p Gene:GRR1(YJR090C)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:HPF1(YOL155C)|FD-Score:-3.8|P-value:7.09E-5||SGD DESC:Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines Gene:IRS4(YKR019C)|FD-Score:-3.14|P-value:8.58E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:IST2(YBR086C)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process Gene:MDM34(YGL219C)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MEH1(YKR007W)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MRPL44(YMR225C)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MTC2(YKL098W)|FD-Score:4.12|P-value:1.91E-5||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:NIT2(YJL126W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:PCL7(YIL050W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Pho85p cyclin of the Pho80p subfamily, forms a functional kinase complex with Pho85p which phosphorylates Mmr1p and is regulated by Pho81p; involved in glycogen metabolism, expression is cell-cycle regulated Gene:PDE1(YGL248W)|FD-Score:-3.87|P-value:5.50E-5||SGD DESC:Low-affinity cyclic AMP phosphodiesterase, controls glucose and intracellular acidification-induced cAMP signaling, target of the cAMP-protein kinase A (PKA) pathway; glucose induces transcription and inhibits translation Gene:PEP3(YLR148W)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PFA5(YDR459C)|FD-Score:-3.48|P-value:2.52E-4||SGD DESC:Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain Gene:PIN2(YOR104W)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated Gene:PSR1(YLL010C)|FD-Score:6.78|P-value:6.14E-12||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:PUS5(YLR165C)|FD-Score:3.86|P-value:5.60E-5||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability Gene:RCO1(YMR075W)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p Gene:RFM1(YOR279C)|FD-Score:4|P-value:3.22E-5||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RPL23B(YER117W)|FD-Score:-4.74|P-value:1.07E-6||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:RRD2(YPL152W)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress Gene:RRP8(YDR083W)|FD-Score:3.76|P-value:8.39E-5||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:SAC1(YKL212W)|FD-Score:5.68|P-value:6.91E-9||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SIT4(YDL047W)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SNQ2(YDR011W)|FD-Score:4.86|P-value:5.93E-7||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:THI74(YDR438W)|FD-Score:-3.14|P-value:8.38E-4||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:TPO4(YOR273C)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRP2(YER090W)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:UBC11(YOR339C)|FD-Score:-3.89|P-value:4.94E-5||SGD DESC:Ubiquitin-conjugating enzyme most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2 Gene:WTM2(YOR229W)|FD-Score:4.2|P-value:1.36E-5||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats Gene:YAL065C(YAL065C_p)|FD-Score:6.1|P-value:5.43E-10||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YBR085C-A(YBR085C-A_p)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus; protein abundance increases in response to DNA replication stress Gene:YCK2(YNL154C)|FD-Score:3.92|P-value:4.44E-5||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck1p in morphogenesis, proper septin assembly, endocytic trafficking; YCK2 has a paralog, YCK1, that arose from the whole genome duplication Gene:YCR064C(YCR064C_d)|FD-Score:-4.27|P-value:9.83E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YCR100C(YCR100C_p)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Putative protein of unknown function Gene:YDR034W-B(YDR034W-B_p)|FD-Score:-3.36|P-value:3.92E-4||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR149C(YDR149C_d)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YDR203W(YDR203W_d)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR535C(YDR535C_d)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YIL001W(YIL001W_p)|FD-Score:5.97|P-value:1.19E-9||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YKL091C(YKL091C)|FD-Score:-3.31|P-value:4.59E-4||SGD DESC:Putative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study Gene:YLR171W(YLR171W_d)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR172C-A(YMR172C-A_d)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR247W-A(YMR247W-A_p)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Putative protein of unknown function Gene:YNL276C(YNL276C_d)|FD-Score:4.5|P-value:3.46E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W Gene:YOL038C-A(YOL038C-A_p)|FD-Score:4.95|P-value:3.62E-7||SGD DESC:Putative protein of unknown function; identified by SAGE analysis Gene:YPL080C(YPL080C_d)|FD-Score:3.74|P-value:9.33E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR096C(YPR096C)|FD-Score:-5.08|P-value:1.84E-7||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:ADE12(YNL220W)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence Gene:AEP2(YMR282C)|FD-Score:-4.08|P-value:2.25E-5||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:ASM4(YDL088C)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication Gene:AST1(YBL069W)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication Gene:ATG11(YPR049C)|FD-Score:6.71|P-value:9.75E-12||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:ATG17(YLR423C)|FD-Score:3.85|P-value:5.90E-5||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:AXL1(YPR122W)|FD-Score:4.53|P-value:2.93E-6||SGD DESC:Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells Gene:BSC6(YOL137W)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Protein of unknown function containing 8 putative transmembrane seqments; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:COX10(YPL172C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:ECL1(YGR146C)|FD-Score:-4.01|P-value:2.98E-5||SGD DESC:Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:ECM30(YLR436C)|FD-Score:-3.37|P-value:3.72E-4||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm Gene:FMP25(YLR077W)|FD-Score:3.1|P-value:9.64E-4||SGD DESC:Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria Gene:GIP1(YBR045C)|FD-Score:-4.14|P-value:1.71E-5||SGD DESC:Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p Gene:GRR1(YJR090C)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:HPF1(YOL155C)|FD-Score:-3.8|P-value:7.09E-5||SGD DESC:Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines Gene:IRS4(YKR019C)|FD-Score:-3.14|P-value:8.58E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:IST2(YBR086C)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process Gene:MDM34(YGL219C)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MEH1(YKR007W)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MRPL44(YMR225C)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MTC2(YKL098W)|FD-Score:4.12|P-value:1.91E-5||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:NIT2(YJL126W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:PCL7(YIL050W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Pho85p cyclin of the Pho80p subfamily, forms a functional kinase complex with Pho85p which phosphorylates Mmr1p and is regulated by Pho81p; involved in glycogen metabolism, expression is cell-cycle regulated Gene:PDE1(YGL248W)|FD-Score:-3.87|P-value:5.50E-5||SGD DESC:Low-affinity cyclic AMP phosphodiesterase, controls glucose and intracellular acidification-induced cAMP signaling, target of the cAMP-protein kinase A (PKA) pathway; glucose induces transcription and inhibits translation Gene:PEP3(YLR148W)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PFA5(YDR459C)|FD-Score:-3.48|P-value:2.52E-4||SGD DESC:Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain Gene:PIN2(YOR104W)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated Gene:PSR1(YLL010C)|FD-Score:6.78|P-value:6.14E-12||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:PUS5(YLR165C)|FD-Score:3.86|P-value:5.60E-5||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability Gene:RCO1(YMR075W)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p Gene:RFM1(YOR279C)|FD-Score:4|P-value:3.22E-5||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RPL23B(YER117W)|FD-Score:-4.74|P-value:1.07E-6||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:RRD2(YPL152W)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress Gene:RRP8(YDR083W)|FD-Score:3.76|P-value:8.39E-5||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:SAC1(YKL212W)|FD-Score:5.68|P-value:6.91E-9||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SIT4(YDL047W)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SNQ2(YDR011W)|FD-Score:4.86|P-value:5.93E-7||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:THI74(YDR438W)|FD-Score:-3.14|P-value:8.38E-4||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:TPO4(YOR273C)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRP2(YER090W)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:UBC11(YOR339C)|FD-Score:-3.89|P-value:4.94E-5||SGD DESC:Ubiquitin-conjugating enzyme most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2 Gene:WTM2(YOR229W)|FD-Score:4.2|P-value:1.36E-5||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats Gene:YAL065C(YAL065C_p)|FD-Score:6.1|P-value:5.43E-10||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YBR085C-A(YBR085C-A_p)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus; protein abundance increases in response to DNA replication stress Gene:YCK2(YNL154C)|FD-Score:3.92|P-value:4.44E-5||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck1p in morphogenesis, proper septin assembly, endocytic trafficking; YCK2 has a paralog, YCK1, that arose from the whole genome duplication Gene:YCR064C(YCR064C_d)|FD-Score:-4.27|P-value:9.83E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YCR100C(YCR100C_p)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Putative protein of unknown function Gene:YDR034W-B(YDR034W-B_p)|FD-Score:-3.36|P-value:3.92E-4||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR149C(YDR149C_d)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YDR203W(YDR203W_d)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR535C(YDR535C_d)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YIL001W(YIL001W_p)|FD-Score:5.97|P-value:1.19E-9||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YKL091C(YKL091C)|FD-Score:-3.31|P-value:4.59E-4||SGD DESC:Putative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study Gene:YLR171W(YLR171W_d)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR172C-A(YMR172C-A_d)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR247W-A(YMR247W-A_p)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Putative protein of unknown function Gene:YNL276C(YNL276C_d)|FD-Score:4.5|P-value:3.46E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W Gene:YOL038C-A(YOL038C-A_p)|FD-Score:4.95|P-value:3.62E-7||SGD DESC:Putative protein of unknown function; identified by SAGE analysis Gene:YPL080C(YPL080C_d)|FD-Score:3.74|P-value:9.33E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR096C(YPR096C)|FD-Score:-5.08|P-value:1.84E-7||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W12.106.22E-345.43LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YHR170W6.661.41E-110.70NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YBR252W5.961.26E-91.50DUT1deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate
YLR145W4.464.06E-60.09RMP1Subunit of RNase MRP, which processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P
YBR192W4.376.14E-60.09RIM2Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
YLR430W4.299.05E-60.57SEN1Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS
YBR049C3.711.03E-40.21REB1RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication
YDR238C3.502.34E-40.06SEC26Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
YJL167W3.442.92E-40.07ERG20Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis
YDR331W3.373.82E-40.10GPI8ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog
YGR073C_d3.265.52E-40.31YGR073C_dDubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein
YIR015W2.950.001580.00RPR2Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress
YPR112C2.950.001590.02MRD1Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region
YIL115C2.930.001700.09NUP159FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p)
YMR203W2.840.002240.05TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLL010C6.786.14E-12PSR1Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p
YPR049C6.719.75E-12ATG11Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS)
YAL065C_p6.105.43E-10YAL065C_pPutative protein of unknown function; has homology to FLO1; possible pseudogene
YIL001W_p5.971.19E-9YIL001W_pPutative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm
YKL212W5.686.91E-9SAC1Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
YOL038C-A_p4.953.62E-7YOL038C-A_pPutative protein of unknown function; identified by SAGE analysis
YDR011W4.865.93E-7SNQ2Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species
YPR122W4.532.93E-6AXL1Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells
YNL276C_d4.503.46E-6YNL276C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W
YOR229W4.201.36E-5WTM2Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats
YBL069W4.151.67E-5AST1Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication
YKL098W4.121.91E-5MTC2Protein of unknown function; mtc2 is synthetically sick with cdc13-1
YOR279C4.003.22E-5RFM1DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance
YOR104W3.924.34E-5PIN2Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated
YNL154C3.924.44E-5YCK2Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck1p in morphogenesis, proper septin assembly, endocytic trafficking; YCK2 has a paralog, YCK1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_3280
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2435.73E-80SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.16417960S ribosome export
0.2389.76E-77SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.084210560S ribosome export
0.2252.08E-68SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.10666760S ribosome export
0.2142.07E-62SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.10256460S ribosome export
0.2138.84E-62SGTC_32769139060 49.5 μMChembridge (Drug-like library)48706190.260S ribosome export
0.2131.17E-61SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.19178160S ribosome export
0.2081.04E-58SGTC_8660495-0101 262.0 μMChemDiv (Drug-like library)6356940.072289260S ribosome export
0.2061.24E-57SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.12345760S ribosome export
0.2021.96E-55SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.094594660S ribosome export
0.1981.69E-53SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.09195460S ribosome export
0.1864.53E-47SGTC_32229128301 49.5 μMChembridge (Drug-like library)49113580.56896560S ribosome export
0.1736.22E-41SGTC_22067254556 147.0 μMChembridge (Fragment library)43989050.0860S ribosome export
0.1718.84E-40SGTC_15924',5'-dihydroxyflavone 8.8 μMTimTec (Pure natural product library)6886690.0860S ribosome export
0.1701.68E-39SGTC_29379044753 11.3 μMChembridge (Drug-like library)64655300.057471360S ribosome export
0.1668.09E-38SGTC_623k072-0108 16.9 μMChemDiv (Drug-like library)53828030.0958904heme biosynthesis & mitochondrial translocase

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3328914062429.04 μM0.6538464904392Chembridge (Drug-like library)335.4463.33104
SGTC_3222912830149.47 μM0.5689654911358Chembridge (Drug-like library)375.390813.9330760S ribosome export
SGTC_3333914182012.09 μM0.5517241939433Chembridge (Drug-like library)349.472583.97404
SGTC_3343914837918.98 μM0.5333331955036Chembridge (Drug-like library)349.472583.96804
SGTC_333191410601.96 μM0.4920634906567Chembridge (Drug-like library)363.843423.84614cell wall
SGTC_3257913920632.98 μM0.4827594910041Chembridge (Drug-like library)321.419423.04804
SGTC_3235913260249.47 μM0.475414912240Chembridge (Drug-like library)352.430163.31605
SGTC_334591484103.56 μM0.4307694903596Chembridge (Drug-like library)413.514783.9715
SGTC_3329914123327.56 μM0.4264714909101Chembridge (Drug-like library)405.416793.39418
SGTC_3339914588314.94 μM0.4117652030670Chembridge (Drug-like library)379.498563.43515