9109332

N-[3-chloro-4-(4-methylpiperidin-1-yl)phenyl]pyridine-3-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3283
Screen concentration 35.8 μM
Source Chembridge (Drug-like library)
PubChem CID 17027281
SMILES CC1CCN(CC1)C2=C(C=C(C=C2)NC(=O)C3=CN=CC=C3)Cl
Standardized SMILES CC1CCN(CC1)c2ccc(NC(=O)c3cccnc3)cc2Cl
Molecular weight 329.8239
ALogP 3.46
H-bond donor count 1
H-bond acceptor count 3
Response signature DNA intercalators

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.17
% growth inhibition (Hom. pool) 8.67


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17027281
Download HIP data (tab-delimited text)  (excel)
Gene:AME1(YBR211C)|FD-Score:-3.27|P-value:5.45E-4|Clearance:0||SGD DESC:Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC3(YLR314C)|FD-Score:-3.09|P-value:9.98E-4|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CEP3(YMR168C)|FD-Score:4.17|P-value:1.54E-5|Clearance:0.06||SGD DESC:Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain Gene:EXO70(YJL085W)|FD-Score:3.2|P-value:6.78E-4|Clearance:0.04||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MES1(YGR264C)|FD-Score:-3.29|P-value:4.99E-4|Clearance:0||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:PGS1(YCL004W)|FD-Score:3.26|P-value:5.56E-4|Clearance:0.06||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:PRE2(YPR103W)|FD-Score:-6.06|P-value:6.74E-10|Clearance:0||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:PTI1(YGR156W)|FD-Score:4.07|P-value:2.31E-5|Clearance:0.53||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RFT1(YBL020W)|FD-Score:-4.74|P-value:1.07E-6|Clearance:0||SGD DESC:Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein Gene:RPC31(YNL151C)|FD-Score:3.16|P-value:7.78E-4|Clearance:0.1||SGD DESC:RNA polymerase III subunit C31 Gene:RPT2(YDL007W)|FD-Score:-3.86|P-value:5.70E-5|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle Gene:RRN7(YJL025W)|FD-Score:3.55|P-value:1.94E-4|Clearance:0.28||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RRS1(YOR294W)|FD-Score:3.27|P-value:5.40E-4|Clearance:0.01||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:SPC42(YKL042W)|FD-Score:4.8|P-value:7.86E-7|Clearance:0.64||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:TAF6(YGL112C)|FD-Score:-3.17|P-value:7.64E-4|Clearance:0||SGD DESC:Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 Gene:TFC6(YDR362C)|FD-Score:5.19|P-value:1.07E-7|Clearance:0.64||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:TIF6(YPR016C)|FD-Score:-3.32|P-value:4.49E-4|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:TRE2(YOR256C)|FD-Score:-3.47|P-value:2.62E-4|Clearance:0||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:YGR265W(YGR265W_d)|FD-Score:4.11|P-value:2.01E-5|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:AME1(YBR211C)|FD-Score:-3.27|P-value:5.45E-4|Clearance:0||SGD DESC:Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC3(YLR314C)|FD-Score:-3.09|P-value:9.98E-4|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CEP3(YMR168C)|FD-Score:4.17|P-value:1.54E-5|Clearance:0.06||SGD DESC:Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain Gene:EXO70(YJL085W)|FD-Score:3.2|P-value:6.78E-4|Clearance:0.04||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MES1(YGR264C)|FD-Score:-3.29|P-value:4.99E-4|Clearance:0||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:PGS1(YCL004W)|FD-Score:3.26|P-value:5.56E-4|Clearance:0.06||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:PRE2(YPR103W)|FD-Score:-6.06|P-value:6.74E-10|Clearance:0||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:PTI1(YGR156W)|FD-Score:4.07|P-value:2.31E-5|Clearance:0.53||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RFT1(YBL020W)|FD-Score:-4.74|P-value:1.07E-6|Clearance:0||SGD DESC:Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein Gene:RPC31(YNL151C)|FD-Score:3.16|P-value:7.78E-4|Clearance:0.1||SGD DESC:RNA polymerase III subunit C31 Gene:RPT2(YDL007W)|FD-Score:-3.86|P-value:5.70E-5|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle Gene:RRN7(YJL025W)|FD-Score:3.55|P-value:1.94E-4|Clearance:0.28||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RRS1(YOR294W)|FD-Score:3.27|P-value:5.40E-4|Clearance:0.01||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:SPC42(YKL042W)|FD-Score:4.8|P-value:7.86E-7|Clearance:0.64||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:TAF6(YGL112C)|FD-Score:-3.17|P-value:7.64E-4|Clearance:0||SGD DESC:Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 Gene:TFC6(YDR362C)|FD-Score:5.19|P-value:1.07E-7|Clearance:0.64||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:TIF6(YPR016C)|FD-Score:-3.32|P-value:4.49E-4|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:TRE2(YOR256C)|FD-Score:-3.47|P-value:2.62E-4|Clearance:0||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:YGR265W(YGR265W_d)|FD-Score:4.11|P-value:2.01E-5|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17027281
Download HOP data (tab-delimited text)  (excel)
Gene:AAC3(YBR085W)|FD-Score:-3.17|P-value:7.52E-4||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication Gene:ABM1(YJR108W)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Protein of unknown function, required for normal microtubule organization Gene:ACH1(YBL015W)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth Gene:ADH7(YCR105W)|FD-Score:-4.37|P-value:6.19E-6||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase with broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance Gene:ADI1(YMR009W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Acireductone dioxygenease involved in the methionine salvage pathway; ortholog of human MTCBP-1; transcribed as polycistronic mRNA with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions Gene:AFT1(YGL071W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AHC1(YOR023C)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex Gene:AMS1(YGL156W)|FD-Score:-4.21|P-value:1.25E-5||SGD DESC:Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway Gene:ARA2(YMR041C)|FD-Score:-3.38|P-value:3.65E-4||SGD DESC:NAD-dependent arabinose dehydrogenase, involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase Gene:ARG5,6(YER069W)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine Gene:ATP10(YLR393W)|FD-Score:-4.6|P-value:2.16E-6||SGD DESC:Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 Gene:ATX2(YOR079C)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Golgi membrane protein involved in manganese homeostasis; overproduction suppresses the sod1 (copper, zinc superoxide dismutase) null mutation Gene:BIO2(YGR286C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:BTS1(YPL069C)|FD-Score:4.09|P-value:2.13E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CDC50(YCR094W)|FD-Score:-3.22|P-value:6.50E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:COA2(YPL189C-A)|FD-Score:-3.89|P-value:5.10E-5||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:COG6(YNL041C)|FD-Score:3.95|P-value:3.85E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CUP2(YGL166W)|FD-Score:-3.4|P-value:3.37E-4||SGD DESC:Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication Gene:DET1(YDR051C)|FD-Score:-3.78|P-value:7.91E-5||SGD DESC:Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel Gene:DMC1(YER179W)|FD-Score:-4.96|P-value:3.46E-7||SGD DESC:Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p Gene:EFM1(YHL039W)|FD-Score:3.91|P-value:4.58E-5||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:EFT1(YOR133W)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication Gene:ENV9(YOR246C)|FD-Score:7.09|P-value:6.68E-13||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:FCY22(YER060W-A)|FD-Score:-3.74|P-value:9.22E-5||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FUM1(YPL262W)|FD-Score:5.04|P-value:2.33E-7||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GAT3(YLR013W)|FD-Score:-3.48|P-value:2.52E-4||SGD DESC:Protein containing GATA family zinc finger motifs Gene:GCS1(YDL226C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GNP1(YDR508C)|FD-Score:-3.22|P-value:6.51E-4||SGD DESC:High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication Gene:GOR1(YNL274C)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Glyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:GRR1(YJR090C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GRX1(YCL035C)|FD-Score:-3.81|P-value:6.82E-5||SGD DESC:Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:GYP6(YJL044C)|FD-Score:-3.22|P-value:6.42E-4||SGD DESC:GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport Gene:HBT1(YDL223C)|FD-Score:3.1|P-value:9.52E-4||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HCR1(YLR192C)|FD-Score:5.4|P-value:3.32E-8||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HEH2(YDR458C)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:HEL1(YKR017C_p)|FD-Score:-4.21|P-value:1.25E-5||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU) Gene:HMX1(YLR205C)|FD-Score:-3.31|P-value:4.62E-4||SGD DESC:ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants Gene:HSP42(YDR171W)|FD-Score:-3.2|P-value:6.94E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:IMP1(YMR150C)|FD-Score:-4.26|P-value:1.01E-5||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:INO1(YJL153C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element Gene:IOC4(YMR044W)|FD-Score:3.99|P-value:3.34E-5||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:IZH4(YOL101C)|FD-Score:3.98|P-value:3.45E-5||SGD DESC:Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress Gene:KTR6(YPL053C)|FD-Score:4.57|P-value:2.47E-6||SGD DESC:Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication Gene:MMS2(YGL087C)|FD-Score:3.15|P-value:8.28E-4||SGD DESC:Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress Gene:MRPL31(YKL138C)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH1(YHR120W)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:NBP2(YDR162C)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NCL1(YBL024W)|FD-Score:-3.3|P-value:4.82E-4||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NEJ1(YLR265C)|FD-Score:-3.85|P-value:5.86E-5||SGD DESC:Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p Gene:PSP1(YDR505C)|FD-Score:-5.03|P-value:2.46E-7||SGD DESC:Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication Gene:PTC5(YOR090C)|FD-Score:-3.99|P-value:3.30E-5||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p Gene:PTP3(YER075C)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm Gene:RNH202(YDR279W)|FD-Score:-3.14|P-value:8.49E-4||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS2 that causes Aicardi-Goutieres syndrome Gene:RPS0A(YGR214W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; Gene:RPS22B(YLR367W)|FD-Score:-3.19|P-value:7.19E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication Gene:RRP8(YDR083W)|FD-Score:-4.41|P-value:5.21E-6||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:RSR1(YGR152C)|FD-Score:4.57|P-value:2.50E-6||SGD DESC:GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases Gene:SEG2(YKL105C_p)|FD-Score:-3.79|P-value:7.42E-5||SGD DESC:Eisosome component and paralog of Seg1p; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes Gene:SKN7(YHR206W)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SNU66(YOR308C)|FD-Score:-3.18|P-value:7.48E-4||SGD DESC:Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 Gene:STE24(YJR117W)|FD-Score:-3.12|P-value:9.17E-4||SGD DESC:Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans Gene:SUB1(YMR039C)|FD-Score:-4.32|P-value:7.87E-6||SGD DESC:Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress Gene:SWC7(YLR385C)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Protein of unknown function, component of the Swr1p complex that incorporates Htz1p into chromatin Gene:TDA2(YER071C_p)|FD-Score:-4.67|P-value:1.51E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele Gene:TES1(YJR019C)|FD-Score:-3.74|P-value:9.38E-5||SGD DESC:Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids Gene:TGS1(YPL157W)|FD-Score:5.47|P-value:2.24E-8||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TOS2(YGR221C)|FD-Score:-3.31|P-value:4.61E-4||SGD DESC:Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p Gene:TRP3(YKL211C)|FD-Score:-9.15|P-value:2.75E-20||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRX1(YLR043C)|FD-Score:4.5|P-value:3.35E-6||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:TSR3(YOR006C)|FD-Score:4.68|P-value:1.46E-6||SGD DESC:Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:VHR2(YER064C_p)|FD-Score:-3.19|P-value:7.17E-4||SGD DESC:Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:VPS21(YOR089C)|FD-Score:-4.88|P-value:5.37E-7||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS62(YGR141W)|FD-Score:3.86|P-value:5.61E-5||SGD DESC:Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins Gene:YBL010C(YBL010C_p)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles Gene:YBL086C(YBL086C_p)|FD-Score:-4.04|P-value:2.69E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR094W(YDR094W_d)|FD-Score:-4.67|P-value:1.53E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2 Gene:YDR095C(YDR095C_d)|FD-Score:-4.74|P-value:1.05E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL043W(YEL043W)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold Gene:YER067C-A(YER067C-A_d)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W Gene:YFR039C(YFR039C_p)|FD-Score:-3.28|P-value:5.26E-4||SGD DESC:Putative protein of unknown function; may be involved in response to high salt and changes in carbon source; deletion mutant has decreased spore survival in Drosophila feces Gene:YGK3(YOL128C)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YGL165C(YGL165C_d)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CUP2/YGL166W Gene:YHR112C(YHR112C)|FD-Score:3.91|P-value:4.69E-5||SGD DESC:Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway Gene:YHR127W(YHR127W)|FD-Score:3.91|P-value:4.65E-5||SGD DESC:Protein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis Gene:YJL068C(YJL068C)|FD-Score:-3.78|P-value:7.84E-5||SGD DESC:Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D Gene:YKL151C(YKL151C)|FD-Score:-4.64|P-value:1.75E-6||SGD DESC:NADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YLR124W(YLR124W_d)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMC1(YPR058W)|FD-Score:-5.47|P-value:2.21E-8||SGD DESC:Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC1 has a paralog, YMC2, that arose from the whole genome duplication Gene:YML122C(YML122C_d)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR031W-A(YMR031W-A_d)|FD-Score:-4.51|P-value:3.22E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR166C(YMR166C_p)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YNL097C-B(YNL097C-B_p)|FD-Score:-3.43|P-value:3.03E-4||SGD DESC:Putative protein of unknown function Gene:YOR012W(YOR012W_p)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Putative protein of unknown function Gene:AAC3(YBR085W)|FD-Score:-3.17|P-value:7.52E-4||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication Gene:ABM1(YJR108W)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Protein of unknown function, required for normal microtubule organization Gene:ACH1(YBL015W)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth Gene:ADH7(YCR105W)|FD-Score:-4.37|P-value:6.19E-6||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase with broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance Gene:ADI1(YMR009W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Acireductone dioxygenease involved in the methionine salvage pathway; ortholog of human MTCBP-1; transcribed as polycistronic mRNA with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions Gene:AFT1(YGL071W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AHC1(YOR023C)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex Gene:AMS1(YGL156W)|FD-Score:-4.21|P-value:1.25E-5||SGD DESC:Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway Gene:ARA2(YMR041C)|FD-Score:-3.38|P-value:3.65E-4||SGD DESC:NAD-dependent arabinose dehydrogenase, involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase Gene:ARG5,6(YER069W)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine Gene:ATP10(YLR393W)|FD-Score:-4.6|P-value:2.16E-6||SGD DESC:Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 Gene:ATX2(YOR079C)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Golgi membrane protein involved in manganese homeostasis; overproduction suppresses the sod1 (copper, zinc superoxide dismutase) null mutation Gene:BIO2(YGR286C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:BTS1(YPL069C)|FD-Score:4.09|P-value:2.13E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CDC50(YCR094W)|FD-Score:-3.22|P-value:6.50E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:COA2(YPL189C-A)|FD-Score:-3.89|P-value:5.10E-5||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:COG6(YNL041C)|FD-Score:3.95|P-value:3.85E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CUP2(YGL166W)|FD-Score:-3.4|P-value:3.37E-4||SGD DESC:Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication Gene:DET1(YDR051C)|FD-Score:-3.78|P-value:7.91E-5||SGD DESC:Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel Gene:DMC1(YER179W)|FD-Score:-4.96|P-value:3.46E-7||SGD DESC:Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p Gene:EFM1(YHL039W)|FD-Score:3.91|P-value:4.58E-5||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:EFT1(YOR133W)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication Gene:ENV9(YOR246C)|FD-Score:7.09|P-value:6.68E-13||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:FCY22(YER060W-A)|FD-Score:-3.74|P-value:9.22E-5||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FUM1(YPL262W)|FD-Score:5.04|P-value:2.33E-7||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GAT3(YLR013W)|FD-Score:-3.48|P-value:2.52E-4||SGD DESC:Protein containing GATA family zinc finger motifs Gene:GCS1(YDL226C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GNP1(YDR508C)|FD-Score:-3.22|P-value:6.51E-4||SGD DESC:High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication Gene:GOR1(YNL274C)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Glyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:GRR1(YJR090C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GRX1(YCL035C)|FD-Score:-3.81|P-value:6.82E-5||SGD DESC:Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:GYP6(YJL044C)|FD-Score:-3.22|P-value:6.42E-4||SGD DESC:GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport Gene:HBT1(YDL223C)|FD-Score:3.1|P-value:9.52E-4||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HCR1(YLR192C)|FD-Score:5.4|P-value:3.32E-8||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HEH2(YDR458C)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:HEL1(YKR017C_p)|FD-Score:-4.21|P-value:1.25E-5||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU) Gene:HMX1(YLR205C)|FD-Score:-3.31|P-value:4.62E-4||SGD DESC:ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants Gene:HSP42(YDR171W)|FD-Score:-3.2|P-value:6.94E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:IMP1(YMR150C)|FD-Score:-4.26|P-value:1.01E-5||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:INO1(YJL153C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element Gene:IOC4(YMR044W)|FD-Score:3.99|P-value:3.34E-5||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:IZH4(YOL101C)|FD-Score:3.98|P-value:3.45E-5||SGD DESC:Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress Gene:KTR6(YPL053C)|FD-Score:4.57|P-value:2.47E-6||SGD DESC:Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication Gene:MMS2(YGL087C)|FD-Score:3.15|P-value:8.28E-4||SGD DESC:Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress Gene:MRPL31(YKL138C)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH1(YHR120W)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:NBP2(YDR162C)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NCL1(YBL024W)|FD-Score:-3.3|P-value:4.82E-4||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NEJ1(YLR265C)|FD-Score:-3.85|P-value:5.86E-5||SGD DESC:Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p Gene:PSP1(YDR505C)|FD-Score:-5.03|P-value:2.46E-7||SGD DESC:Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication Gene:PTC5(YOR090C)|FD-Score:-3.99|P-value:3.30E-5||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p Gene:PTP3(YER075C)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm Gene:RNH202(YDR279W)|FD-Score:-3.14|P-value:8.49E-4||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS2 that causes Aicardi-Goutieres syndrome Gene:RPS0A(YGR214W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; Gene:RPS22B(YLR367W)|FD-Score:-3.19|P-value:7.19E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication Gene:RRP8(YDR083W)|FD-Score:-4.41|P-value:5.21E-6||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:RSR1(YGR152C)|FD-Score:4.57|P-value:2.50E-6||SGD DESC:GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases Gene:SEG2(YKL105C_p)|FD-Score:-3.79|P-value:7.42E-5||SGD DESC:Eisosome component and paralog of Seg1p; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes Gene:SKN7(YHR206W)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SNU66(YOR308C)|FD-Score:-3.18|P-value:7.48E-4||SGD DESC:Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 Gene:STE24(YJR117W)|FD-Score:-3.12|P-value:9.17E-4||SGD DESC:Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans Gene:SUB1(YMR039C)|FD-Score:-4.32|P-value:7.87E-6||SGD DESC:Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress Gene:SWC7(YLR385C)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Protein of unknown function, component of the Swr1p complex that incorporates Htz1p into chromatin Gene:TDA2(YER071C_p)|FD-Score:-4.67|P-value:1.51E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele Gene:TES1(YJR019C)|FD-Score:-3.74|P-value:9.38E-5||SGD DESC:Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids Gene:TGS1(YPL157W)|FD-Score:5.47|P-value:2.24E-8||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TOS2(YGR221C)|FD-Score:-3.31|P-value:4.61E-4||SGD DESC:Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p Gene:TRP3(YKL211C)|FD-Score:-9.15|P-value:2.75E-20||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRX1(YLR043C)|FD-Score:4.5|P-value:3.35E-6||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:TSR3(YOR006C)|FD-Score:4.68|P-value:1.46E-6||SGD DESC:Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:VHR2(YER064C_p)|FD-Score:-3.19|P-value:7.17E-4||SGD DESC:Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:VPS21(YOR089C)|FD-Score:-4.88|P-value:5.37E-7||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS62(YGR141W)|FD-Score:3.86|P-value:5.61E-5||SGD DESC:Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins Gene:YBL010C(YBL010C_p)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles Gene:YBL086C(YBL086C_p)|FD-Score:-4.04|P-value:2.69E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR094W(YDR094W_d)|FD-Score:-4.67|P-value:1.53E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2 Gene:YDR095C(YDR095C_d)|FD-Score:-4.74|P-value:1.05E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL043W(YEL043W)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold Gene:YER067C-A(YER067C-A_d)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W Gene:YFR039C(YFR039C_p)|FD-Score:-3.28|P-value:5.26E-4||SGD DESC:Putative protein of unknown function; may be involved in response to high salt and changes in carbon source; deletion mutant has decreased spore survival in Drosophila feces Gene:YGK3(YOL128C)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YGL165C(YGL165C_d)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CUP2/YGL166W Gene:YHR112C(YHR112C)|FD-Score:3.91|P-value:4.69E-5||SGD DESC:Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway Gene:YHR127W(YHR127W)|FD-Score:3.91|P-value:4.65E-5||SGD DESC:Protein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis Gene:YJL068C(YJL068C)|FD-Score:-3.78|P-value:7.84E-5||SGD DESC:Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D Gene:YKL151C(YKL151C)|FD-Score:-4.64|P-value:1.75E-6||SGD DESC:NADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YLR124W(YLR124W_d)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMC1(YPR058W)|FD-Score:-5.47|P-value:2.21E-8||SGD DESC:Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC1 has a paralog, YMC2, that arose from the whole genome duplication Gene:YML122C(YML122C_d)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR031W-A(YMR031W-A_d)|FD-Score:-4.51|P-value:3.22E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR166C(YMR166C_p)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YNL097C-B(YNL097C-B_p)|FD-Score:-3.43|P-value:3.03E-4||SGD DESC:Putative protein of unknown function Gene:YOR012W(YOR012W_p)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR362C5.191.07E-70.64TFC6One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110
YKL042W4.807.86E-70.64SPC42Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane
YMR168C4.171.54E-50.06CEP3Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
YGR265W_d4.112.01E-50.03YGR265W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase
YGR156W4.072.31E-50.53PTI1Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
YJL025W3.551.94E-40.28RRN7Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
YOR294W3.275.40E-40.01RRS1Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice
YCL004W3.265.56E-40.06PGS1Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis
YJL085W3.206.78E-40.04EXO70Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
YNL151C3.167.78E-40.10RPC31RNA polymerase III subunit C31
YHR172W3.060.001090.09SPC97Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YER125W2.970.001470.06RSP5E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p
YDL058W2.920.001760.00USO1Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
YLR051C2.920.001770.03FCF2Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon
YMR208W2.890.001930.11ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR246C7.096.68E-13ENV9Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
YPL157W5.472.24E-8TGS1Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs
YLR192C5.403.32E-8HCR1Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA
YPL262W5.042.33E-7FUM1Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria
YOR006C4.681.46E-6TSR3Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress
YPL053C4.572.47E-6KTR6Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication
YGR152C4.572.50E-6RSR1GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases
YLR043C4.503.35E-6TRX1Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress
YMR166C_p4.231.16E-5YMR166C_pPredicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
YHR120W4.221.22E-5MSH1DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis
YPL069C4.092.13E-5BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YOR133W4.042.70E-5EFT1Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication
YDR458C4.032.81E-5HEH2Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle
YMR044W3.993.34E-5IOC4Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing
YOL101C3.983.45E-5IZH4Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress

GO enrichment analysis for SGTC_3283
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2631.05E-93SGTC_1926methyl fluorone black 53.6 μMTimTec (Natural product derivative library)727210.0657895DNA intercalators
0.1881.10E-47SGTC_30409091894 49.5 μMChembridge (Drug-like library)252369660.166667DNA intercalators
0.1791.26E-43SGTC_264promethazine 12.5 mMMiscellaneous60140.1DNA intercalators
0.1674.35E-38SGTC_1107amlodipine 47.0 μMNIH Clinical Collection21620.139785
0.1653.95E-37SGTC_4711-acyl-paf 4.0 μMICCB bioactive library167593670.043956DNA intercalators
0.1632.42E-36SGTC_14153966-0071 78.1 μMChemDiv (Drug-like library)28931850.15625DNA intercalators
0.1511.82E-31SGTC_1453737-0113 38.4 μMChemDiv (Drug-like library)68493510.060241DNA intercalators
0.1449.46E-29SGTC_1459k035-0031 35.7 μMChemDiv (Drug-like library)38771280.0882353DNA intercalators
0.1374.96E-26SGTC_12900986-0249 10.2 μMChemDiv (Drug-like library)533290.0985916DNA intercalators
0.1351.94E-25SGTC_512prima-1 270.0 μMICCB bioactive library3229680.107692DNA intercalators
0.1322.63E-24SGTC_219mitoxanthrone 164.4 μMMiscellaneous42120.0675676DNA intercalators
0.1317.64E-24SGTC_1444161-2749 112.4 μMChemDiv (Drug-like library)7975010.131579DNA intercalators
0.1133.30E-18SGTC_8670518-0118 155.0 μMChemDiv (Drug-like library)68481580.108108DNA intercalators
0.0961.21E-13SGTC_15153460-0031 42.1 μMChemDiv (Drug-like library)28765960.137931DNA intercalators
0.0961.93E-13SGTC_259usnic acid 108.9 μMMiscellaneous56460.0823529

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2854902455971.43 μM0.5468753163529Chembridge (Drug-like library)341.361161.16916
SGTC_2967908953849.85 μM0.517241728216Chembridge (Drug-like library)300.663633.0715
SGTC_8480368-008985.6 μM0.438596762535ChemDiv (Drug-like library)267.110722.79212iron homeostasis
SGTC_2795778001054.55 μM0.419355953673Chembridge (Drug-like library)312.3812434.15113
SGTC_3010908008971.43 μM0.40909117027233Chembridge (Drug-like library)378.2387833.49714
SGTC_1035k081-004155.2 μM0.390625731709ChemDiv (Drug-like library)341.58772.8613Golgi
SGTC_1462k081-001426.5 μM0.3870973270634ChemDiv (Drug-like library)329.5521833.08213
SGTC_2943906852945.32 μM0.3870973270634Yeast Active Library329.5521833.08213
SGTC_7891309-113570.6 μM0.3753092424ChemDiv (Drug-like library)379.068643.80623ERG2
SGTC_2800788793581.82 μM0.3692312961002Chembridge (Drug-like library)340.826624.24613RPP1 & pyrimidine depletion