9108171

N-(3-chloro-4-methylphenyl)-2-[4-(2-methoxyphenyl)piperazin-1-yl]acetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3284
Screen concentration 64.9 μM
Source Chembridge (Drug-like library)
PubChem CID 8687505
SMILES CC1=C(C=C(C=C1)NC(=O)CN2CCN(CC2)C3=CC=CC=C3OC)Cl
Standardized SMILES COc1ccccc1N2CCN(CC(=O)Nc3ccc(C)c(Cl)c3)CC2
Molecular weight 373.8765
ALogP 3.72
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.69
% growth inhibition (Hom. pool) 10.61


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 8687505
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:5.66|P-value:7.73E-9|Clearance:0.73||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ARP3(YJR065C)|FD-Score:3.33|P-value:4.31E-4|Clearance:0.12||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:CDC15(YAR019C)|FD-Score:-3.1|P-value:9.62E-4|Clearance:0||SGD DESC:Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress Gene:CDC20(YGL116W)|FD-Score:3.12|P-value:8.90E-4|Clearance:0.05||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC39(YCR093W)|FD-Score:4.81|P-value:7.62E-7|Clearance:0.58||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:DAD1(YDR016C)|FD-Score:-5.94|P-value:1.44E-9|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:FMN1(YDR236C)|FD-Score:3.43|P-value:3.00E-4|Clearance:0.06||SGD DESC:Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane Gene:FRS1(YLR060W)|FD-Score:3.16|P-value:7.95E-4|Clearance:0.03||SGD DESC:Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control Gene:IRA1(YBR140C)|FD-Score:5.54|P-value:1.49E-8|Clearance:0.73||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:PRP4(YPR178W)|FD-Score:4.23|P-value:1.19E-5|Clearance:0.68||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RKI1(YOR095C)|FD-Score:3.18|P-value:7.29E-4|Clearance:0.03||SGD DESC:Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis Gene:RPO21(YDL140C)|FD-Score:3.21|P-value:6.68E-4|Clearance:0.03||SGD DESC:RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime Gene:RSC58(YLR033W)|FD-Score:-3.42|P-value:3.14E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SEC21(YNL287W)|FD-Score:3.37|P-value:3.80E-4|Clearance:0.03||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:TFC3(YAL001C)|FD-Score:-5.31|P-value:5.58E-8|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TPI1(YDR050C)|FD-Score:3.55|P-value:1.95E-4|Clearance:0.12||SGD DESC:Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human TPI causes a rare autosomal disease Gene:UFE1(YOR075W)|FD-Score:-3.45|P-value:2.77E-4|Clearance:0||SGD DESC:t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER Gene:ACT1(YFL039C)|FD-Score:5.66|P-value:7.73E-9|Clearance:0.73||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ARP3(YJR065C)|FD-Score:3.33|P-value:4.31E-4|Clearance:0.12||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:CDC15(YAR019C)|FD-Score:-3.1|P-value:9.62E-4|Clearance:0||SGD DESC:Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress Gene:CDC20(YGL116W)|FD-Score:3.12|P-value:8.90E-4|Clearance:0.05||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC39(YCR093W)|FD-Score:4.81|P-value:7.62E-7|Clearance:0.58||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:DAD1(YDR016C)|FD-Score:-5.94|P-value:1.44E-9|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:FMN1(YDR236C)|FD-Score:3.43|P-value:3.00E-4|Clearance:0.06||SGD DESC:Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane Gene:FRS1(YLR060W)|FD-Score:3.16|P-value:7.95E-4|Clearance:0.03||SGD DESC:Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control Gene:IRA1(YBR140C)|FD-Score:5.54|P-value:1.49E-8|Clearance:0.73||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:PRP4(YPR178W)|FD-Score:4.23|P-value:1.19E-5|Clearance:0.68||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RKI1(YOR095C)|FD-Score:3.18|P-value:7.29E-4|Clearance:0.03||SGD DESC:Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis Gene:RPO21(YDL140C)|FD-Score:3.21|P-value:6.68E-4|Clearance:0.03||SGD DESC:RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime Gene:RSC58(YLR033W)|FD-Score:-3.42|P-value:3.14E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SEC21(YNL287W)|FD-Score:3.37|P-value:3.80E-4|Clearance:0.03||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:TFC3(YAL001C)|FD-Score:-5.31|P-value:5.58E-8|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TPI1(YDR050C)|FD-Score:3.55|P-value:1.95E-4|Clearance:0.12||SGD DESC:Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human TPI causes a rare autosomal disease Gene:UFE1(YOR075W)|FD-Score:-3.45|P-value:2.77E-4|Clearance:0||SGD DESC:t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 8687505
Download HOP data (tab-delimited text)  (excel)
Gene:ACF4(YJR083C)|FD-Score:3.75|P-value:9.02E-5||SGD DESC:Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate Gene:ALG8(YOR067C)|FD-Score:4.37|P-value:6.26E-6||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:APD1(YBR151W)|FD-Score:-3.84|P-value:6.12E-5||SGD DESC:Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus Gene:ARL3(YPL051W)|FD-Score:-3.73|P-value:9.76E-5||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:BTS1(YPL069C)|FD-Score:3.72|P-value:9.87E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD17(YNR027W)|FD-Score:4.42|P-value:4.88E-6||SGD DESC:Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) Gene:BZZ1(YHR114W)|FD-Score:-3.48|P-value:2.52E-4||SGD DESC:SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins Gene:CBP3(YPL215W)|FD-Score:-3.48|P-value:2.55E-4||SGD DESC:Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex Gene:CSG2(YBR036C)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CYB5(YNL111C)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DAL2(YIR029W)|FD-Score:4.6|P-value:2.12E-6||SGD DESC:Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAL81(YIR023W)|FD-Score:4.8|P-value:7.99E-7||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DIA1(YMR316W)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:ERV14(YGL054C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FAA1(YOR317W)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FMP41(YNL168C_p)|FD-Score:6.13|P-value:4.53E-10||SGD DESC:Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FYV8(YGR196C)|FD-Score:-3.96|P-value:3.72E-5||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GAL11(YOL051W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GCN4(YEL009C)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GUP1(YGL084C)|FD-Score:5.55|P-value:1.43E-8||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HBS1(YKR084C)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay Gene:HNT3(YOR258W)|FD-Score:-4.87|P-value:5.67E-7||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:IMD3(YLR432W)|FD-Score:-3.23|P-value:6.29E-4||SGD DESC:Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed Gene:IOC2(YLR095C)|FD-Score:-3.27|P-value:5.46E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc4p that exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing, contains a PHD finger motif Gene:IRC11(YOR013W_d)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Dubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci Gene:KAP120(YPL125W)|FD-Score:3.75|P-value:8.87E-5||SGD DESC:Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p Gene:KIN1(YDR122W)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p Gene:LAG2(YOL025W)|FD-Score:4.54|P-value:2.86E-6||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:LSB3(YFR024C-A)|FD-Score:4.75|P-value:9.94E-7||SGD DESC:Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication Gene:MBP1(YDL056W)|FD-Score:-5.35|P-value:4.30E-8||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MDM32(YOR147W)|FD-Score:4.64|P-value:1.74E-6||SGD DESC:Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 Gene:MUM2(YBR057C)|FD-Score:3.19|P-value:7.21E-4||SGD DESC:Cytoplasmic protein essential for meiotic DNA replication and sporulation; interacts with Orc2p, which is a component of the origin recognition complex Gene:NEJ1(YLR265C)|FD-Score:-3.12|P-value:8.94E-4||SGD DESC:Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p Gene:PBS2(YJL128C)|FD-Score:-4|P-value:3.15E-5||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PCP1(YGR101W)|FD-Score:-4.01|P-value:3.08E-5||SGD DESC:Mitochondrial serine protease required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases Gene:PDR11(YIL013C)|FD-Score:-4.24|P-value:1.11E-5||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:PEX30(YLR324W)|FD-Score:3.84|P-value:6.16E-5||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PGD1(YGL025C)|FD-Score:6.07|P-value:6.21E-10||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PMP3(YDR276C)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:PTC5(YOR090C)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p Gene:RCY1(YJL204C)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RED1(YLR263W)|FD-Score:-3.55|P-value:1.92E-4||SGD DESC:Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p Gene:REG1(YDR028C)|FD-Score:4.59|P-value:2.20E-6||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RFU1(YLR073C)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes Gene:RGP1(YDR137W)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-3.17|P-value:7.61E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RRT1(YBL048W_d)|FD-Score:-3.42|P-value:3.10E-4||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:SHM2(YLR058C)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:STP22(YCL008C)|FD-Score:4|P-value:3.22E-5||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SUR4(YLR372W)|FD-Score:4.51|P-value:3.25E-6||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWA2(YDR320C)|FD-Score:-6.25|P-value:2.08E-10||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TDA10(YGR205W)|FD-Score:-3.35|P-value:3.99E-4||SGD DESC:ATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other small kinases; null mutant is sensitive to expression of the top1-T722A allele Gene:TRP1(YDR007W)|FD-Score:8.9|P-value:2.73E-19||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:4.61|P-value:2.00E-6||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:7.18|P-value:3.40E-13||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:VPS45(YGL095C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS61(YDR136C_d)|FD-Score:-3.21|P-value:6.71E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YAL004W(YAL004W_d)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Gene:YBR141C(YBR141C_p)|FD-Score:-3.33|P-value:4.35E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:6.17|P-value:3.36E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR248C(YDR248C_p)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Putative gluconokinase; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1 Gene:YDR444W(YDR444W_p)|FD-Score:-3.28|P-value:5.21E-4||SGD DESC:Putative hydrolase acting on ester bonds Gene:YDR524C-B(YDR524C-B_p)|FD-Score:4.13|P-value:1.78E-5||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YGL034C(YGL034C_d)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL117W(YGL117W_p)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Putative protein of unknown function Gene:YHL015W-A(YHL015W-A_p)|FD-Score:-3.19|P-value:7.20E-4||SGD DESC:Putative protein of unknown function Gene:YKR018C(YKR018C_p)|FD-Score:3.8|P-value:7.15E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YLR111W(YLR111W_d)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML034C-A(YML034C-A_d)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML122C(YML122C_d)|FD-Score:3.81|P-value:6.96E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR304C-A(YOR304C-A_p)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck Gene:YPK1(YKL126W)|FD-Score:9.1|P-value:4.33E-20||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL073C(YPL073C_d)|FD-Score:4.67|P-value:1.53E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Gene:YPL150W(YPL150W_p)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Protein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study Gene:YPR098C(YPR098C)|FD-Score:5.29|P-value:6.25E-8||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YPT35(YHR105W)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:ZAP1(YJL056C)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:ACF4(YJR083C)|FD-Score:3.75|P-value:9.02E-5||SGD DESC:Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate Gene:ALG8(YOR067C)|FD-Score:4.37|P-value:6.26E-6||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:APD1(YBR151W)|FD-Score:-3.84|P-value:6.12E-5||SGD DESC:Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus Gene:ARL3(YPL051W)|FD-Score:-3.73|P-value:9.76E-5||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:BTS1(YPL069C)|FD-Score:3.72|P-value:9.87E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD17(YNR027W)|FD-Score:4.42|P-value:4.88E-6||SGD DESC:Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) Gene:BZZ1(YHR114W)|FD-Score:-3.48|P-value:2.52E-4||SGD DESC:SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins Gene:CBP3(YPL215W)|FD-Score:-3.48|P-value:2.55E-4||SGD DESC:Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex Gene:CSG2(YBR036C)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CYB5(YNL111C)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DAL2(YIR029W)|FD-Score:4.6|P-value:2.12E-6||SGD DESC:Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAL81(YIR023W)|FD-Score:4.8|P-value:7.99E-7||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DIA1(YMR316W)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:ERV14(YGL054C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FAA1(YOR317W)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FMP41(YNL168C_p)|FD-Score:6.13|P-value:4.53E-10||SGD DESC:Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FYV8(YGR196C)|FD-Score:-3.96|P-value:3.72E-5||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GAL11(YOL051W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GCN4(YEL009C)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GUP1(YGL084C)|FD-Score:5.55|P-value:1.43E-8||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HBS1(YKR084C)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay Gene:HNT3(YOR258W)|FD-Score:-4.87|P-value:5.67E-7||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:IMD3(YLR432W)|FD-Score:-3.23|P-value:6.29E-4||SGD DESC:Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed Gene:IOC2(YLR095C)|FD-Score:-3.27|P-value:5.46E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc4p that exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing, contains a PHD finger motif Gene:IRC11(YOR013W_d)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Dubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci Gene:KAP120(YPL125W)|FD-Score:3.75|P-value:8.87E-5||SGD DESC:Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p Gene:KIN1(YDR122W)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p Gene:LAG2(YOL025W)|FD-Score:4.54|P-value:2.86E-6||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:LSB3(YFR024C-A)|FD-Score:4.75|P-value:9.94E-7||SGD DESC:Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication Gene:MBP1(YDL056W)|FD-Score:-5.35|P-value:4.30E-8||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MDM32(YOR147W)|FD-Score:4.64|P-value:1.74E-6||SGD DESC:Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 Gene:MUM2(YBR057C)|FD-Score:3.19|P-value:7.21E-4||SGD DESC:Cytoplasmic protein essential for meiotic DNA replication and sporulation; interacts with Orc2p, which is a component of the origin recognition complex Gene:NEJ1(YLR265C)|FD-Score:-3.12|P-value:8.94E-4||SGD DESC:Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p Gene:PBS2(YJL128C)|FD-Score:-4|P-value:3.15E-5||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PCP1(YGR101W)|FD-Score:-4.01|P-value:3.08E-5||SGD DESC:Mitochondrial serine protease required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases Gene:PDR11(YIL013C)|FD-Score:-4.24|P-value:1.11E-5||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:PEX30(YLR324W)|FD-Score:3.84|P-value:6.16E-5||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PGD1(YGL025C)|FD-Score:6.07|P-value:6.21E-10||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PMP3(YDR276C)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:PTC5(YOR090C)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p Gene:RCY1(YJL204C)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RED1(YLR263W)|FD-Score:-3.55|P-value:1.92E-4||SGD DESC:Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p Gene:REG1(YDR028C)|FD-Score:4.59|P-value:2.20E-6||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RFU1(YLR073C)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes Gene:RGP1(YDR137W)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-3.17|P-value:7.61E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RRT1(YBL048W_d)|FD-Score:-3.42|P-value:3.10E-4||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:SHM2(YLR058C)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:STP22(YCL008C)|FD-Score:4|P-value:3.22E-5||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SUR4(YLR372W)|FD-Score:4.51|P-value:3.25E-6||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWA2(YDR320C)|FD-Score:-6.25|P-value:2.08E-10||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TDA10(YGR205W)|FD-Score:-3.35|P-value:3.99E-4||SGD DESC:ATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other small kinases; null mutant is sensitive to expression of the top1-T722A allele Gene:TRP1(YDR007W)|FD-Score:8.9|P-value:2.73E-19||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:4.61|P-value:2.00E-6||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:7.18|P-value:3.40E-13||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:VPS45(YGL095C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS61(YDR136C_d)|FD-Score:-3.21|P-value:6.71E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YAL004W(YAL004W_d)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Gene:YBR141C(YBR141C_p)|FD-Score:-3.33|P-value:4.35E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:6.17|P-value:3.36E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR248C(YDR248C_p)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Putative gluconokinase; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1 Gene:YDR444W(YDR444W_p)|FD-Score:-3.28|P-value:5.21E-4||SGD DESC:Putative hydrolase acting on ester bonds Gene:YDR524C-B(YDR524C-B_p)|FD-Score:4.13|P-value:1.78E-5||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YGL034C(YGL034C_d)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL117W(YGL117W_p)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Putative protein of unknown function Gene:YHL015W-A(YHL015W-A_p)|FD-Score:-3.19|P-value:7.20E-4||SGD DESC:Putative protein of unknown function Gene:YKR018C(YKR018C_p)|FD-Score:3.8|P-value:7.15E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YLR111W(YLR111W_d)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML034C-A(YML034C-A_d)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML122C(YML122C_d)|FD-Score:3.81|P-value:6.96E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR304C-A(YOR304C-A_p)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck Gene:YPK1(YKL126W)|FD-Score:9.1|P-value:4.33E-20||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL073C(YPL073C_d)|FD-Score:4.67|P-value:1.53E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Gene:YPL150W(YPL150W_p)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Protein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study Gene:YPR098C(YPR098C)|FD-Score:5.29|P-value:6.25E-8||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YPT35(YHR105W)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:ZAP1(YJL056C)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains

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Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YFL039C5.667.73E-90.73ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YBR140C5.541.49E-80.73IRA1GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication
YCR093W4.817.62E-70.58CDC39Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
YPR178W4.231.19E-50.68PRP4Splicing factor, component of the U4/U6-U5 snRNP complex
YDR050C3.551.95E-40.12TPI1Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human TPI causes a rare autosomal disease
YDR236C3.433.00E-40.06FMN1Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane
YNL287W3.373.80E-40.04SEC21Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo
YJR065C3.334.31E-40.12ARP3Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
YDL140C3.216.68E-40.03RPO21RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
YOR095C3.187.29E-40.03RKI1Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis
YLR060W3.167.95E-40.03FRS1Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control
YGL116W3.128.90E-40.05CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YDR301W3.070.001070.07CFT1RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF
YLR277C3.000.001330.01YSH1Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication
YDR498C2.990.001390.00SEC20Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL126W9.104.33E-20YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YDR007W8.902.73E-19TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YDR354W7.183.40E-13TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YDR008C_d6.173.36E-10YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YNL168C_p6.134.53E-10FMP41_pPutative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YGL025C6.076.21E-10PGD1Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
YGL084C5.551.43E-8GUP1Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport
YPR098C5.296.25E-8YPR098CProtein of unknown function, localized to the mitochondrial outer membrane
YJL056C5.191.03E-7ZAP1Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains
YIR023W4.807.99E-7DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YFR024C-A4.759.94E-7LSB3Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication
YPL073C_d4.671.53E-6YPL073C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments
YOR147W4.641.74E-6MDM32Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34
YKL211C4.612.00E-6TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YIR029W4.602.12E-6DAL2Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation

GO enrichment analysis for SGTC_3284
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3073.98E-129SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)30151890.0136986
0.2812.18E-107SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.118421
0.2762.19E-103SGTC_29399044578 71.4 μMChembridge (Drug-like library)26536340.8
0.2753.68E-103SGTC_2524avocatin b 10.6 μMMicrosource (Natural product library)67107480.0136986
0.2663.54E-96SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.138889
0.2665.50E-96SGTC_1824st053723 68.6 μMTimTec (Natural product derivative library)6752250.121622
0.2626.99E-93SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0408163
0.2506.14E-85SGTC_23849036245 200.0 μMChembridge (Fragment library)64627660.188406
0.2421.87E-79SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0609756
0.2417.35E-79SGTC_2669eugenol 100.0 μMMicrosource (Natural product library)33140.166667
0.2391.46E-77SGTC_14964469-0713 57.4 μMChemDiv (Drug-like library)7466910.134146
0.2381.49E-76SGTC_24885268135 45.7 μMMiscellaneous12703560.08
0.2361.35E-75SGTC_14113909-8223 150.0 μMChemDiv (Drug-like library)X14110.120879
0.2361.40E-75SGTC_24785763493 32.3 μMMiscellaneous13655760.102564
0.2351.09E-74SGTC_2741miltefosine 3.0 μMMiscellaneous35990.0547945

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2939904457871.43 μM0.82653634Chembridge (Drug-like library)377.8403433.43915
SGTC_3208911411549.47 μM0.661017650895Chembridge (Drug-like library)357.4218633.26115
SGTC_3202911376149.47 μM0.6229514798415Chembridge (Drug-like library)377.8403433.43915
SGTC_14544239-005442.6 μM0.6129031340767ChemDiv (Drug-like library)432.354044.05214
SGTC_14454239-015697.7 μM0.5806454008346ChemDiv (Drug-like library)375.4123263.22816
SGTC_3177910750649.47 μM0.5762718897194Chembridge (Drug-like library)314.809263.76512ERG2
SGTC_3178910750649.47 μM0.5762718897194Chembridge (Drug-like library)314.809263.76512
SGTC_14464239-019062 μM0.56251340085ChemDiv (Drug-like library)379.495263.87414
SGTC_3179910780149.47 μM0.4307694818259Chembridge (Drug-like library)318.7731433.48413
SGTC_2914130-127835.5 μM0.4264711068044ChemDiv (Drug-like library)375.806223.41505Golgi
SGTC_2783773816571.43 μM0.4262317201110Chembridge (Drug-like library)289.756743.55612