9117135

4-ethyl-5-methyl-2-[[2-(4-methylphenoxy)acetyl]amino]thiophene-3-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3286
Screen concentration 37.2 μM
Source Chembridge (Drug-like library)
PubChem CID 3438150
SMILES CCC1=C(SC(=C1C(=O)N)NC(=O)COC2=CC=C(C=C2)C)C
Standardized SMILES CCc1c(C)sc(NC(=O)COc2ccc(C)cc2)c1C(=O)N
Molecular weight 332.4173
ALogP 2.81
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.52
% growth inhibition (Hom. pool) 14.08


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3438150
Download HIP data (tab-delimited text)  (excel)
Gene:APC5(YOR249C)|FD-Score:3.3|P-value:4.89E-4|Clearance:0.11||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:BMS1(YPL217C)|FD-Score:3.88|P-value:5.17E-5|Clearance:0.06||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CDC48(YDL126C)|FD-Score:-5.16|P-value:1.23E-7|Clearance:0||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:JIP5(YPR169W)|FD-Score:3.17|P-value:7.53E-4|Clearance:0.13||SGD DESC:Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein Gene:MCM5(YLR274W)|FD-Score:5.45|P-value:2.55E-8|Clearance:0.44||SGD DESC:Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase Gene:MTR3(YGR158C)|FD-Score:3.31|P-value:4.65E-4|Clearance:0.01||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NUP49(YGL172W)|FD-Score:4.04|P-value:2.69E-5|Clearance:0.01||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:NUP85(YJR042W)|FD-Score:3.82|P-value:6.73E-5|Clearance:0.37||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:RLP7(YNL002C)|FD-Score:4.55|P-value:2.73E-6|Clearance:0.01||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RPB10(YOR210W)|FD-Score:4.23|P-value:1.16E-5|Clearance:0.19||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPB7(YDR404C)|FD-Score:4.3|P-value:8.48E-6|Clearance:0.07||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RRP42(YDL111C)|FD-Score:4.73|P-value:1.10E-6|Clearance:0.19||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:RRP45(YDR280W)|FD-Score:4.54|P-value:2.86E-6|Clearance:0.23||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:RRP46(YGR095C)|FD-Score:3.45|P-value:2.81E-4|Clearance:0.02||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:TFC3(YAL001C)|FD-Score:5.54|P-value:1.47E-8|Clearance:0.44||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TIF34(YMR146C)|FD-Score:5.17|P-value:1.16E-7|Clearance:0.44||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:TIF35(YDR429C)|FD-Score:5.47|P-value:2.19E-8|Clearance:0.44||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TOM40(YMR203W)|FD-Score:3.43|P-value:3.02E-4|Clearance:0.12||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:YNL114C(YNL114C_d)|FD-Score:3.18|P-value:7.26E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YPR136C(YPR136C_d)|FD-Score:4.03|P-value:2.76E-5|Clearance:0.15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 Gene:APC5(YOR249C)|FD-Score:3.3|P-value:4.89E-4|Clearance:0.11||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:BMS1(YPL217C)|FD-Score:3.88|P-value:5.17E-5|Clearance:0.06||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CDC48(YDL126C)|FD-Score:-5.16|P-value:1.23E-7|Clearance:0||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:JIP5(YPR169W)|FD-Score:3.17|P-value:7.53E-4|Clearance:0.13||SGD DESC:Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein Gene:MCM5(YLR274W)|FD-Score:5.45|P-value:2.55E-8|Clearance:0.44||SGD DESC:Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase Gene:MTR3(YGR158C)|FD-Score:3.31|P-value:4.65E-4|Clearance:0.01||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NUP49(YGL172W)|FD-Score:4.04|P-value:2.69E-5|Clearance:0.01||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:NUP85(YJR042W)|FD-Score:3.82|P-value:6.73E-5|Clearance:0.37||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:RLP7(YNL002C)|FD-Score:4.55|P-value:2.73E-6|Clearance:0.01||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RPB10(YOR210W)|FD-Score:4.23|P-value:1.16E-5|Clearance:0.19||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPB7(YDR404C)|FD-Score:4.3|P-value:8.48E-6|Clearance:0.07||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RRP42(YDL111C)|FD-Score:4.73|P-value:1.10E-6|Clearance:0.19||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:RRP45(YDR280W)|FD-Score:4.54|P-value:2.86E-6|Clearance:0.23||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:RRP46(YGR095C)|FD-Score:3.45|P-value:2.81E-4|Clearance:0.02||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:TFC3(YAL001C)|FD-Score:5.54|P-value:1.47E-8|Clearance:0.44||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TIF34(YMR146C)|FD-Score:5.17|P-value:1.16E-7|Clearance:0.44||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:TIF35(YDR429C)|FD-Score:5.47|P-value:2.19E-8|Clearance:0.44||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TOM40(YMR203W)|FD-Score:3.43|P-value:3.02E-4|Clearance:0.12||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:YNL114C(YNL114C_d)|FD-Score:3.18|P-value:7.26E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YPR136C(YPR136C_d)|FD-Score:4.03|P-value:2.76E-5|Clearance:0.15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3438150
Download HOP data (tab-delimited text)  (excel)
Gene:AIM17(YHL021C)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:ARO1(YDR127W)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:BEM4(YPL161C)|FD-Score:-3.2|P-value:6.80E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BUB3(YOR026W)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p Gene:CBR1(YIL043C)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CCM1(YGR150C)|FD-Score:3.09|P-value:9.92E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CCR4(YAL021C)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:COG8(YML071C)|FD-Score:-4.87|P-value:5.59E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSF1(YLR087C)|FD-Score:-3.77|P-value:8.32E-5||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DBF2(YGR092W)|FD-Score:-3.71|P-value:1.02E-4||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DBP7(YKR024C)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DIT2(YDR402C)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:N-formyltyrosine oxidase, sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s Gene:DPH5(YLR172C)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm Gene:EAR1(YMR171C)|FD-Score:5.87|P-value:2.15E-9||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p Gene:ELP2(YGR200C)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:ERC1(YHR032W)|FD-Score:-3.28|P-value:5.16E-4||SGD DESC:Member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine Gene:ERG3(YLR056W)|FD-Score:-4.4|P-value:5.40E-6||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:GDB1(YPR184W)|FD-Score:3.12|P-value:9.19E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GET2(YER083C)|FD-Score:-5.03|P-value:2.51E-7||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GET3(YDL100C)|FD-Score:-3.22|P-value:6.41E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:HMG2(YLR450W)|FD-Score:-4.4|P-value:5.53E-6||SGD DESC:HMG-CoA reductase; converts HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; one of two isozymes; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks; forms foci at the nuclear periphery upon DNA replication stress Gene:HMI1(YOL095C)|FD-Score:-3.43|P-value:3.03E-4||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:HOS2(YGL194C)|FD-Score:-3.15|P-value:8.11E-4||SGD DESC:Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS Gene:HRT3(YLR097C)|FD-Score:-3.1|P-value:9.60E-4||SGD DESC:Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies Gene:IES1(YFL013C)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:INP51(YIL002C)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:IOC2(YLR095C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc4p that exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing, contains a PHD finger motif Gene:IRC24(YIR036C_p)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:-4.3|P-value:8.44E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISA1(YLL027W)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:KTR6(YPL053C)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication Gene:LHS1(YKL073W)|FD-Score:-3.97|P-value:3.54E-5||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:MET1(YKR069W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MIC17(YMR002W)|FD-Score:3.75|P-value:8.97E-5||SGD DESC:Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MRE11(YMR224C)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:MSN5(YDR335W)|FD-Score:-3.91|P-value:4.57E-5||SGD DESC:Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:MST1(YKL194C)|FD-Score:-3.75|P-value:8.90E-5||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:MSY1(YPL097W)|FD-Score:3.3|P-value:4.75E-4||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:MUM2(YBR057C)|FD-Score:3.83|P-value:6.36E-5||SGD DESC:Cytoplasmic protein essential for meiotic DNA replication and sporulation; interacts with Orc2p, which is a component of the origin recognition complex Gene:NBP2(YDR162C)|FD-Score:-5.99|P-value:1.03E-9||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NDE1(YMR145C)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain Gene:NHX1(YDR456W)|FD-Score:-3.1|P-value:9.58E-4||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:PHO88(YBR106W)|FD-Score:3.92|P-value:4.48E-5||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PMT5(YDL093W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt3p, can instead interact with Pmt2p in some conditions; target for new antifungals Gene:PRO2(YOR323C)|FD-Score:6.03|P-value:8.06E-10||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RAD52(YML032C)|FD-Score:4.56|P-value:2.61E-6||SGD DESC:Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis Gene:ROY1(YMR258C)|FD-Score:4.12|P-value:1.92E-5||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RPL1A(YPL220W)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Ribosomal 60S subunit protein L1A; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal Gene:RPL36B(YPL249C-A)|FD-Score:3.77|P-value:8.01E-5||SGD DESC:Ribosomal 60S subunit protein L36B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36B has a paralog, RPL36A, that arose from the whole genome duplication Gene:RPS21A(YKR057W)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication Gene:RRF1(YHR038W)|FD-Score:3.98|P-value:3.47E-5||SGD DESC:Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria Gene:RRT13(YER066W_p)|FD-Score:-8.17|P-value:1.51E-16||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription Gene:RSR1(YGR152C)|FD-Score:-4.24|P-value:1.11E-5||SGD DESC:GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases Gene:SAS3(YBL052C)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SCW4(YGR279C)|FD-Score:4.32|P-value:7.81E-6||SGD DESC:Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating Gene:SFB3(YHR098C)|FD-Score:-3.09|P-value:9.96E-4||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SLT2(YHR030C)|FD-Score:3.77|P-value:8.17E-5||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SOL4(YGR248W)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication Gene:SRS2(YJL092W)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability Gene:SSD1(YDR293C)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STO1(YMR125W)|FD-Score:-4.5|P-value:3.34E-6||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SWE1(YJL187C)|FD-Score:-3.91|P-value:4.62E-5||SGD DESC:Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate Gene:SYS1(YJL004C)|FD-Score:-3.38|P-value:3.62E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TAT1(YBR069C)|FD-Score:-4.53|P-value:3.01E-6||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TIF2(YJL138C)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication Gene:TNA1(YGR260W)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:High affinity nicotinic acid plasma membrane permease, responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA) Gene:TOM5(YPR133W-A)|FD-Score:3.11|P-value:9.47E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore Gene:TPN1(YGL186C)|FD-Score:-5.92|P-value:1.57E-9||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRE1(YPL176C)|FD-Score:-3.73|P-value:9.75E-5||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:4.32|P-value:7.73E-6||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TSC3(YBR058C-A)|FD-Score:4.81|P-value:7.67E-7||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBP1(YDL122W)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:UGA3(YDL170W)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:UPS2(YLR168C)|FD-Score:4.02|P-value:2.97E-5||SGD DESC:Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI Gene:UTR1(YJR049C)|FD-Score:3.82|P-value:6.72E-5||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VAM6(YDL077C)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VPS55(YJR044C)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:YBR141C(YBR141C_p)|FD-Score:-4.06|P-value:2.48E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YDL176W(YDL176W)|FD-Score:-3.73|P-value:9.66E-5||SGD DESC:Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene Gene:YDR290W(YDR290W_d)|FD-Score:-5.5|P-value:1.88E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YDR455C(YDR455C_d)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YIL001W(YIL001W_p)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YJR018W(YJR018W_d)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL023W(YKL023W_p)|FD-Score:3.74|P-value:9.06E-5||SGD DESC:Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLR290C(YLR290C_p)|FD-Score:-3.28|P-value:5.17E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR290C is not an essential gene Gene:YLR460C(YLR460C_p)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YML084W(YML084W_d)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML096W(YML096W_p)|FD-Score:3.76|P-value:8.66E-5||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YMR206W(YMR206W_p)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Putative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication Gene:YOL038C-A(YOL038C-A_p)|FD-Score:-4.69|P-value:1.34E-6||SGD DESC:Putative protein of unknown function; identified by SAGE analysis Gene:YOR296W(YOR296W_p)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; expressed during copper starvation; YOR296W is not an essential gene Gene:YOR364W(YOR364W_d)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C Gene:YPR098C(YPR098C)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YPT53(YNL093W)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication Gene:AIM17(YHL021C)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:ARO1(YDR127W)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:BEM4(YPL161C)|FD-Score:-3.2|P-value:6.80E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BUB3(YOR026W)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p Gene:CBR1(YIL043C)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CCM1(YGR150C)|FD-Score:3.09|P-value:9.92E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CCR4(YAL021C)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:COG8(YML071C)|FD-Score:-4.87|P-value:5.59E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSF1(YLR087C)|FD-Score:-3.77|P-value:8.32E-5||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DBF2(YGR092W)|FD-Score:-3.71|P-value:1.02E-4||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DBP7(YKR024C)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DIT2(YDR402C)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:N-formyltyrosine oxidase, sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s Gene:DPH5(YLR172C)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm Gene:EAR1(YMR171C)|FD-Score:5.87|P-value:2.15E-9||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p Gene:ELP2(YGR200C)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:ERC1(YHR032W)|FD-Score:-3.28|P-value:5.16E-4||SGD DESC:Member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine Gene:ERG3(YLR056W)|FD-Score:-4.4|P-value:5.40E-6||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:GDB1(YPR184W)|FD-Score:3.12|P-value:9.19E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GET2(YER083C)|FD-Score:-5.03|P-value:2.51E-7||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GET3(YDL100C)|FD-Score:-3.22|P-value:6.41E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:HMG2(YLR450W)|FD-Score:-4.4|P-value:5.53E-6||SGD DESC:HMG-CoA reductase; converts HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; one of two isozymes; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks; forms foci at the nuclear periphery upon DNA replication stress Gene:HMI1(YOL095C)|FD-Score:-3.43|P-value:3.03E-4||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:HOS2(YGL194C)|FD-Score:-3.15|P-value:8.11E-4||SGD DESC:Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS Gene:HRT3(YLR097C)|FD-Score:-3.1|P-value:9.60E-4||SGD DESC:Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies Gene:IES1(YFL013C)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:INP51(YIL002C)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:IOC2(YLR095C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc4p that exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing, contains a PHD finger motif Gene:IRC24(YIR036C_p)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:-4.3|P-value:8.44E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISA1(YLL027W)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:KTR6(YPL053C)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication Gene:LHS1(YKL073W)|FD-Score:-3.97|P-value:3.54E-5||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:MET1(YKR069W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MIC17(YMR002W)|FD-Score:3.75|P-value:8.97E-5||SGD DESC:Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MRE11(YMR224C)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:MSN5(YDR335W)|FD-Score:-3.91|P-value:4.57E-5||SGD DESC:Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:MST1(YKL194C)|FD-Score:-3.75|P-value:8.90E-5||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:MSY1(YPL097W)|FD-Score:3.3|P-value:4.75E-4||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:MUM2(YBR057C)|FD-Score:3.83|P-value:6.36E-5||SGD DESC:Cytoplasmic protein essential for meiotic DNA replication and sporulation; interacts with Orc2p, which is a component of the origin recognition complex Gene:NBP2(YDR162C)|FD-Score:-5.99|P-value:1.03E-9||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NDE1(YMR145C)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain Gene:NHX1(YDR456W)|FD-Score:-3.1|P-value:9.58E-4||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:PHO88(YBR106W)|FD-Score:3.92|P-value:4.48E-5||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PMT5(YDL093W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt3p, can instead interact with Pmt2p in some conditions; target for new antifungals Gene:PRO2(YOR323C)|FD-Score:6.03|P-value:8.06E-10||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RAD52(YML032C)|FD-Score:4.56|P-value:2.61E-6||SGD DESC:Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis Gene:ROY1(YMR258C)|FD-Score:4.12|P-value:1.92E-5||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RPL1A(YPL220W)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Ribosomal 60S subunit protein L1A; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal Gene:RPL36B(YPL249C-A)|FD-Score:3.77|P-value:8.01E-5||SGD DESC:Ribosomal 60S subunit protein L36B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36B has a paralog, RPL36A, that arose from the whole genome duplication Gene:RPS21A(YKR057W)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication Gene:RRF1(YHR038W)|FD-Score:3.98|P-value:3.47E-5||SGD DESC:Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria Gene:RRT13(YER066W_p)|FD-Score:-8.17|P-value:1.51E-16||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription Gene:RSR1(YGR152C)|FD-Score:-4.24|P-value:1.11E-5||SGD DESC:GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases Gene:SAS3(YBL052C)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SCW4(YGR279C)|FD-Score:4.32|P-value:7.81E-6||SGD DESC:Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating Gene:SFB3(YHR098C)|FD-Score:-3.09|P-value:9.96E-4||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SLT2(YHR030C)|FD-Score:3.77|P-value:8.17E-5||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SOL4(YGR248W)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication Gene:SRS2(YJL092W)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability Gene:SSD1(YDR293C)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STO1(YMR125W)|FD-Score:-4.5|P-value:3.34E-6||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SWE1(YJL187C)|FD-Score:-3.91|P-value:4.62E-5||SGD DESC:Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate Gene:SYS1(YJL004C)|FD-Score:-3.38|P-value:3.62E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TAT1(YBR069C)|FD-Score:-4.53|P-value:3.01E-6||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TIF2(YJL138C)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication Gene:TNA1(YGR260W)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:High affinity nicotinic acid plasma membrane permease, responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA) Gene:TOM5(YPR133W-A)|FD-Score:3.11|P-value:9.47E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore Gene:TPN1(YGL186C)|FD-Score:-5.92|P-value:1.57E-9||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRE1(YPL176C)|FD-Score:-3.73|P-value:9.75E-5||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:4.32|P-value:7.73E-6||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TSC3(YBR058C-A)|FD-Score:4.81|P-value:7.67E-7||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBP1(YDL122W)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:UGA3(YDL170W)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:UPS2(YLR168C)|FD-Score:4.02|P-value:2.97E-5||SGD DESC:Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI Gene:UTR1(YJR049C)|FD-Score:3.82|P-value:6.72E-5||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VAM6(YDL077C)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VPS55(YJR044C)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:YBR141C(YBR141C_p)|FD-Score:-4.06|P-value:2.48E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YDL176W(YDL176W)|FD-Score:-3.73|P-value:9.66E-5||SGD DESC:Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene Gene:YDR290W(YDR290W_d)|FD-Score:-5.5|P-value:1.88E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YDR455C(YDR455C_d)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YIL001W(YIL001W_p)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YJR018W(YJR018W_d)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL023W(YKL023W_p)|FD-Score:3.74|P-value:9.06E-5||SGD DESC:Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLR290C(YLR290C_p)|FD-Score:-3.28|P-value:5.17E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR290C is not an essential gene Gene:YLR460C(YLR460C_p)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YML084W(YML084W_d)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML096W(YML096W_p)|FD-Score:3.76|P-value:8.66E-5||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YMR206W(YMR206W_p)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Putative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication Gene:YOL038C-A(YOL038C-A_p)|FD-Score:-4.69|P-value:1.34E-6||SGD DESC:Putative protein of unknown function; identified by SAGE analysis Gene:YOR296W(YOR296W_p)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; expressed during copper starvation; YOR296W is not an essential gene Gene:YOR364W(YOR364W_d)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C Gene:YPR098C(YPR098C)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YPT53(YNL093W)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YAL001C5.541.47E-80.44TFC3Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding
YDR429C5.472.19E-80.44TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
YLR274W5.452.55E-80.44MCM5Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase
YMR146C5.171.16E-70.44TIF34eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation
YDL111C4.731.10E-60.19RRP42Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
YNL002C4.552.73E-60.01RLP7Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs
YDR280W4.542.86E-60.23RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YDR404C4.308.48E-60.07RPB7RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
YOR210W4.231.16E-50.19RPB10RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
YGL172W4.042.69E-50.01NUP49FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p)
YPR136C_d4.032.76E-50.15YPR136C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9
YPL217C3.885.17E-50.06BMS1GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p
YJR042W3.826.73E-50.37NUP85Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75
YGR095C3.452.81E-40.02RRP46Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
YMR203W3.433.02E-40.12TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR323C6.038.06E-10PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YMR171C5.872.15E-9EAR1Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p
YBR058C-A4.817.67E-7TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YMR224C4.711.23E-6MRE11Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress
YML032C4.562.61E-6RAD52Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis
YER090W4.327.73E-6TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YGR279C4.327.81E-6SCW4Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating
YIL001W_p4.221.23E-5YIL001W_pPutative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm
YMR258C4.121.92E-5ROY1GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p
YDR127W4.111.99E-5ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YLR168C4.022.97E-5UPS2Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI
YHR038W3.983.47E-5RRF1Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria
YBR106W3.924.48E-5PHO88Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations
YBR057C3.836.36E-5MUM2Cytoplasmic protein essential for meiotic DNA replication and sporulation; interacts with Orc2p, which is a component of the origin recognition complex
YJR049C3.826.72E-5UTR1ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_3286
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1352.45E-25SGTC_2751anetholtrithion 17.2 μMMiscellaneous21940.112903
0.1157.65E-19SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0422535TSC3-RPN4
0.1151.12E-18SGTC_1919st067113 52.3 μMTimTec (Natural product derivative library)12682430.119048TSC3-RPN4
0.1111.28E-17SGTC_25787,8-dihydroxyflavone 64.4 μMMicrosource (Natural product library)18800.056338
0.1093.63E-17SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.0857143TSC3-RPN4
0.1071.46E-16SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.0512821TSC3-RPN4
0.1055.08E-16SGTC_2501sodium tetradecyl sulfate 13.4 μMMicrosource (Natural product library)236657720.0704225
0.1023.60E-15SGTC_16985-fluorocytosine 1.1 μMTimTec (Natural product derivative library)33660.0655738exosome
0.1001.13E-14SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.128571TSC3-RPN4
0.0977.19E-14SGTC_9262268-1401 59.4 μMChemDiv (Drug-like library)29315780.102564TSC3-RPN4
0.0961.28E-13SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.0588235TSC3-RPN4
0.0944.56E-13SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0857143TSC3-RPN4
0.0937.70E-13SGTC_13893405-0187 19.0 μMChemDiv (Drug-like library)7998900.147059
0.0921.86E-12SGTC_22186965428 193.0 μMChembridge (Fragment library)6756840.339286TSC3-RPN4
0.0912.20E-12SGTC_6173770-0098 35.0 μMChemDiv (Drug-like library)59626390.1125exosome

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2217693479996.07 μM0.442308675606Chembridge (Fragment library)240.321941.49323TSC3-RPN4
SGTC_3022908799349.47 μM0.36923117173644Chembridge (Drug-like library)351.422182.40224
SGTC_22067254556146.97 μM0.3620694398905Chembridge (Fragment library)275.326220.8842460S ribosome export
SGTC_3151909982149.47 μM0.35294117174189Chembridge (Drug-like library)329.416662.03624
SGTC_22186965428192.98 μM0.339286675684Chembridge (Fragment library)238.306061.12823TSC3-RPN4
SGTC_1300136-0219337.66 μM0.333333234244ChemDiv (Drug-like library)296.148643.77812
SGTC_7950685-026578 μM0.333333302754ChemDiv (Drug-like library)340.599643.86212excess fatty acid
SGTC_1935st07453241.6 μM0.3275862829742TimTec (Natural product derivative library)272.295843.55724
SGTC_2826799254119.48 μM0.3275862985066Chembridge (Drug-like library)297.6845063.52514
SGTC_8251988-222489.6 μM0.3235291180859ChemDiv (Drug-like library)399.25962.86316cell wall signaling