9117173

N-(3-methoxypropyl)-2-phenylquinazolin-4-amine hydrochloride

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3287
Screen concentration 37.1 μM
Source Chembridge (Drug-like library)
PubChem CID 2789709
SMILES COCCCNC1=NC(=NC2=CC=CC=C21)C3=CC=CC=C3.Cl
Standardized SMILES COCCCNc1nc(nc2ccccc12)c3ccccc3
Molecular weight 329.8239
ALogP 3.67
H-bond donor count 2
H-bond acceptor count 4
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.19
% growth inhibition (Hom. pool) 10.46


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2789709
Download HIP data (tab-delimited text)  (excel)
Gene:CCT2(YIL142W)|FD-Score:-3.46|P-value:2.70E-4|Clearance:0||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT4(YDL143W)|FD-Score:-3.25|P-value:5.86E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:-3.13|P-value:8.74E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:DIS3(YOL021C)|FD-Score:-3.89|P-value:4.99E-5|Clearance:0||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:ERG11(YHR007C)|FD-Score:-4.33|P-value:7.30E-6|Clearance:0||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:LSG1(YGL099W)|FD-Score:3.59|P-value:1.65E-4|Clearance:0.24||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MAS1(YLR163C)|FD-Score:-3.58|P-value:1.70E-4|Clearance:0||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:NIP1(YMR309C)|FD-Score:-6.1|P-value:5.45E-10|Clearance:0||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NOP2(YNL061W)|FD-Score:-4.35|P-value:6.80E-6|Clearance:0||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:PDC2(YDR081C)|FD-Score:-3.15|P-value:8.03E-4|Clearance:0||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PRP4(YPR178W)|FD-Score:6.17|P-value:3.37E-10|Clearance:2.25||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPA43(YOR340C)|FD-Score:-3.69|P-value:1.13E-4|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:RPB10(YOR210W)|FD-Score:-4.87|P-value:5.60E-7|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPC10(YHR143W-A)|FD-Score:-3.56|P-value:1.87E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:RPL10(YLR075W)|FD-Score:-3.82|P-value:6.79E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPL17A(YKL180W)|FD-Score:-6.42|P-value:6.83E-11|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS2(YGL123W)|FD-Score:-3.79|P-value:7.67E-5|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RPS3(YNL178W)|FD-Score:-4.85|P-value:6.33E-7|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP9(YPR137W)|FD-Score:-3.14|P-value:8.53E-4|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:RSA4(YCR072C)|FD-Score:3.92|P-value:4.48E-5|Clearance:0.33||SGD DESC:WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus Gene:SPN1(YPR133C)|FD-Score:-3.62|P-value:1.47E-4|Clearance:0||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:TFC3(YAL001C)|FD-Score:-3.18|P-value:7.44E-4|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TIF35(YDR429C)|FD-Score:-3.61|P-value:1.50E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:UTP4(YDR324C)|FD-Score:-3.36|P-value:3.97E-4|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YDR355C(YDR355C_d)|FD-Score:3.35|P-value:3.98E-4|Clearance:0.68||SGD DESC:Dubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W Gene:YDR526C(YDR526C_d)|FD-Score:-3.18|P-value:7.35E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR136C(YPR136C_d)|FD-Score:-4.04|P-value:2.67E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 Gene:CCT2(YIL142W)|FD-Score:-3.46|P-value:2.70E-4|Clearance:0||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT4(YDL143W)|FD-Score:-3.25|P-value:5.86E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:-3.13|P-value:8.74E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:DIS3(YOL021C)|FD-Score:-3.89|P-value:4.99E-5|Clearance:0||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:ERG11(YHR007C)|FD-Score:-4.33|P-value:7.30E-6|Clearance:0||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:LSG1(YGL099W)|FD-Score:3.59|P-value:1.65E-4|Clearance:0.24||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MAS1(YLR163C)|FD-Score:-3.58|P-value:1.70E-4|Clearance:0||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:NIP1(YMR309C)|FD-Score:-6.1|P-value:5.45E-10|Clearance:0||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NOP2(YNL061W)|FD-Score:-4.35|P-value:6.80E-6|Clearance:0||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:PDC2(YDR081C)|FD-Score:-3.15|P-value:8.03E-4|Clearance:0||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PRP4(YPR178W)|FD-Score:6.17|P-value:3.37E-10|Clearance:2.25||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPA43(YOR340C)|FD-Score:-3.69|P-value:1.13E-4|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:RPB10(YOR210W)|FD-Score:-4.87|P-value:5.60E-7|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPC10(YHR143W-A)|FD-Score:-3.56|P-value:1.87E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:RPL10(YLR075W)|FD-Score:-3.82|P-value:6.79E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPL17A(YKL180W)|FD-Score:-6.42|P-value:6.83E-11|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS2(YGL123W)|FD-Score:-3.79|P-value:7.67E-5|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RPS3(YNL178W)|FD-Score:-4.85|P-value:6.33E-7|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP9(YPR137W)|FD-Score:-3.14|P-value:8.53E-4|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:RSA4(YCR072C)|FD-Score:3.92|P-value:4.48E-5|Clearance:0.33||SGD DESC:WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus Gene:SPN1(YPR133C)|FD-Score:-3.62|P-value:1.47E-4|Clearance:0||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:TFC3(YAL001C)|FD-Score:-3.18|P-value:7.44E-4|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TIF35(YDR429C)|FD-Score:-3.61|P-value:1.50E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:UTP4(YDR324C)|FD-Score:-3.36|P-value:3.97E-4|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YDR355C(YDR355C_d)|FD-Score:3.35|P-value:3.98E-4|Clearance:0.68||SGD DESC:Dubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W Gene:YDR526C(YDR526C_d)|FD-Score:-3.18|P-value:7.35E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR136C(YPR136C_d)|FD-Score:-4.04|P-value:2.67E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2789709
Download HOP data (tab-delimited text)  (excel)
Gene:ATP14(YLR295C)|FD-Score:-4.48|P-value:3.75E-6||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:BNA4(YBL098W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease Gene:BRE1(YDL074C)|FD-Score:-3.48|P-value:2.50E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:CSF1(YLR087C)|FD-Score:-3.99|P-value:3.25E-5||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GIM5(YML094W)|FD-Score:-5|P-value:2.85E-7||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:HMG2(YLR450W)|FD-Score:4.67|P-value:1.52E-6||SGD DESC:HMG-CoA reductase; converts HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; one of two isozymes; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks; forms foci at the nuclear periphery upon DNA replication stress Gene:IRC24(YIR036C_p)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci Gene:IZH4(YOL101C)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress Gene:KIN1(YDR122W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p Gene:MET6(YER091C)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Cobalamin-independent methionine synthase, involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs Gene:NOT5(YPR072W)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:PCL1(YNL289W)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth Gene:PGD1(YGL025C)|FD-Score:-3.15|P-value:8.21E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:QRI5(YLR204W)|FD-Score:-3.55|P-value:1.90E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:SEH1(YGL100W)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SKI3(YPR189W)|FD-Score:3.73|P-value:9.49E-5||SGD DESC:Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:VIP1(YLR410W)|FD-Score:3.16|P-value:7.98E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; IP7 production is important for phosphate signaling; involved in cortical actin cytoskeleton function, and invasive pseudohyphal growth analogous to S. pombe asp1 Gene:VMA13(YPR036W)|FD-Score:-3.3|P-value:4.78E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS3(YDR495C)|FD-Score:-5.01|P-value:2.75E-7||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS45(YGL095C)|FD-Score:-5.74|P-value:4.76E-9||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS62(YGR141W)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins Gene:WAR1(YML076C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively Gene:YCR102C(YCR102C_p)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YDL073W(YDL073W_p)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Putative protein of unknown function; YDL073W is not an essential gene Gene:YGL042C(YGL042C_d)|FD-Score:-3.33|P-value:4.37E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1 Gene:YLR072W(YLR072W_p)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene Gene:YLR290C(YLR290C_p)|FD-Score:5.97|P-value:1.17E-9||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR290C is not an essential gene Gene:YMC2(YBR104W)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC2 has a paralog, YMC1, that arose from the whole genome duplication Gene:YMR102C(YMR102C_p)|FD-Score:3.98|P-value:3.51E-5||SGD DESC:Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene Gene:YNR071C(YNR071C_p)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Putative aldose 1-epimerase Gene:ATP14(YLR295C)|FD-Score:-4.48|P-value:3.75E-6||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:BNA4(YBL098W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease Gene:BRE1(YDL074C)|FD-Score:-3.48|P-value:2.50E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:CSF1(YLR087C)|FD-Score:-3.99|P-value:3.25E-5||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GIM5(YML094W)|FD-Score:-5|P-value:2.85E-7||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:HMG2(YLR450W)|FD-Score:4.67|P-value:1.52E-6||SGD DESC:HMG-CoA reductase; converts HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; one of two isozymes; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks; forms foci at the nuclear periphery upon DNA replication stress Gene:IRC24(YIR036C_p)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci Gene:IZH4(YOL101C)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress Gene:KIN1(YDR122W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p Gene:MET6(YER091C)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Cobalamin-independent methionine synthase, involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs Gene:NOT5(YPR072W)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:PCL1(YNL289W)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth Gene:PGD1(YGL025C)|FD-Score:-3.15|P-value:8.21E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:QRI5(YLR204W)|FD-Score:-3.55|P-value:1.90E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:SEH1(YGL100W)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SKI3(YPR189W)|FD-Score:3.73|P-value:9.49E-5||SGD DESC:Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:VIP1(YLR410W)|FD-Score:3.16|P-value:7.98E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; IP7 production is important for phosphate signaling; involved in cortical actin cytoskeleton function, and invasive pseudohyphal growth analogous to S. pombe asp1 Gene:VMA13(YPR036W)|FD-Score:-3.3|P-value:4.78E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS3(YDR495C)|FD-Score:-5.01|P-value:2.75E-7||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS45(YGL095C)|FD-Score:-5.74|P-value:4.76E-9||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS62(YGR141W)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins Gene:WAR1(YML076C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively Gene:YCR102C(YCR102C_p)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YDL073W(YDL073W_p)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Putative protein of unknown function; YDL073W is not an essential gene Gene:YGL042C(YGL042C_d)|FD-Score:-3.33|P-value:4.37E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1 Gene:YLR072W(YLR072W_p)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene Gene:YLR290C(YLR290C_p)|FD-Score:5.97|P-value:1.17E-9||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR290C is not an essential gene Gene:YMC2(YBR104W)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC2 has a paralog, YMC1, that arose from the whole genome duplication Gene:YMR102C(YMR102C_p)|FD-Score:3.98|P-value:3.51E-5||SGD DESC:Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene Gene:YNR071C(YNR071C_p)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Putative aldose 1-epimerase

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR178W6.173.37E-102.25PRP4Splicing factor, component of the U4/U6-U5 snRNP complex
YCR072C3.924.48E-50.33RSA4WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus
YGL099W3.591.65E-40.24LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YDR355C_d3.353.98E-40.68YDR355C_dDubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W
YJR065C2.680.003720.08ARP3Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
YLR106C2.590.004750.01MDN1Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus
YDR180W2.580.004940.09SCC2Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin
YPL128C2.490.006370.07TBF1Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation
YDL221W_d2.420.007830.06YDL221W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13
YFL045C2.350.009310.04SEC53Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen
YDR328C2.320.010200.02SKP1Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
YML125C2.300.010800.08PGA3Putative cytochrome b5 reductase, localized to the plasma membrane; may be involved in regulation of lifespan; required for maturation of Gas1p and Pho8p, proposed to be involved in protein trafficking; PGA3 has a paralog, AIM33, that arose from the whole genome duplication
YKL036C_d2.210.013500.10YKL036C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W
YNL260C_p2.110.017500.04LTO1_pEssential protein that forms a complex with Rli1p and Yae1p; ortholog of human ORAOV1, which is overexpressed in solid tumors; inviability of null mutant under standard conditions is complemented by overexpression of ORAOV1; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species
YJL061W2.070.019400.01NUP82Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR290C_p5.971.17E-9YLR290C_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR290C is not an essential gene
YLR450W4.671.52E-6HMG2HMG-CoA reductase; converts HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; one of two isozymes; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks; forms foci at the nuclear periphery upon DNA replication stress
YLR072W_p4.181.48E-5YLR072W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene
YER091C4.032.81E-5MET6Cobalamin-independent methionine synthase, involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs
YMR102C_p3.983.51E-5YMR102C_pProtein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene
YPR189W3.739.49E-5SKI3Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay
YDR122W3.621.49E-4KIN1Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p
YGR141W3.581.70E-4VPS62Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins
YBL098W3.551.93E-4BNA4Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease
YML076C3.472.59E-4WAR1Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively
YBR104W3.472.60E-4YMC2Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC2 has a paralog, YMC1, that arose from the whole genome duplication
YNR071C_p3.393.46E-4YNR071C_pPutative aldose 1-epimerase
YIR036C_p3.383.56E-4IRC24_pPutative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci
YOL101C3.383.66E-4IZH4Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress
YNL289W3.285.12E-4PCL1Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth

GO enrichment analysis for SGTC_3287
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1897.82E-49SGTC_490phenamil 164.0 μMMiscellaneous47550.134328heme requiring
0.1878.04E-48SGTC_15924',5'-dihydroxyflavone 8.8 μMTimTec (Pure natural product library)6886690.12121260S ribosome export
0.1843.60E-46SGTC_2739tacrine 100.9 μMMiscellaneous19350.222222heme requiring
0.1623.34E-36SGTC_1786berberine 21.6 μMMiscellaneous124560.090909160S ribosome export
0.1611.86E-35SGTC_14053909-7894 12.0 μMChemDiv (Drug-like library)71040600.71111160S ribosome export
0.1612.51E-35SGTC_1619st003326 88.7 μMTimTec (Natural product derivative library)28490320.16393460S ribosome export
0.1583.78E-34SGTC_1827berberine 7.2 μMTimTec (Natural product derivative library)6294130.090909160S ribosome export
0.1554.41E-33SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.15151560S ribosome export
0.1491.06E-30SGTC_1829berberine 14.4 μMTimTec (Natural product derivative library)23530.090909160S ribosome export
0.1491.11E-30SGTC_12690831-0796 3.0 μMChemDiv (Drug-like library)9277580.086956560S ribosome export
0.1352.29E-25SGTC_32229128301 49.5 μMChembridge (Drug-like library)49113580.15068560S ribosome export
0.1323.15E-24SGTC_14063909-7906 11.9 μMChemDiv (Drug-like library)X14060.83720960S ribosome export
0.1301.06E-23SGTC_2645abamectin 100.0 μMMicrosource (Natural product library)57022080.0458015heme requiring
0.1285.46E-23SGTC_1522st012842 45.6 μMTimTec (Pure natural product library)34910670.154762RNA pol III & RNase P/MRP
0.1261.93E-22SGTC_14043909-7820 48.3 μMChemDiv (Drug-like library)237413730.39655260S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_14063909-790611.9 μM0.837209X1406ChemDiv (Drug-like library)307.389543.7734160S ribosome export
SGTC_14053909-789412 μM0.7111117104060ChemDiv (Drug-like library)293.362963.7111460S ribosome export
SGTC_14033909-781562.9 μM0.4406786615483ChemDiv (Drug-like library)389.4222035.11216
SGTC_1983454-287316.19 μM0.431373710957ChemDiv (Drug-like library)297.782044.7461360S ribosome export
SGTC_14073909-790912.3 μM0.4237294411780ChemDiv (Drug-like library)319.400244.2011460S ribosome export
SGTC_14043909-782048.3 μM0.39655223741373ChemDiv (Drug-like library)371.431745.551560S ribosome export
SGTC_4253825-701713.1 μM0.3859653397440ChemDiv (Drug-like library)329.3702435.78914
SGTC_8103825-7150143 μM0.37704922330411ChemDiv (Drug-like library)415.48436.021660S ribosome export
SGTC_9103454-3003490 μM0.365385820824ChemDiv (Drug-like library)299.326023.95604
SGTC_1824st05372368.6 μM0.358491675225TimTec (Natural product derivative library)291.347083.39704