9121177

1-(5-chloro-2-methylphenyl)-5-methyl-N-(pyridin-3-ylmethyl)triazole-4-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3291
Screen concentration 71.4 μM
Source Chembridge (Drug-like library)
PubChem CID 17015612
SMILES CC1=C(C=C(C=C1)Cl)N2C(=C(N=N2)C(=O)NCC3=CN=CC=C3)C
Standardized SMILES Cc1ccc(Cl)cc1n2nnc(C(=O)NCc3cccnc3)c2C
Molecular weight 341.7948
ALogP 2.95
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.45
% growth inhibition (Hom. pool) 3.43


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17015612
Download HIP data (tab-delimited text)  (excel)
Gene:ALG2(YGL065C)|FD-Score:3.43|P-value:2.97E-4|Clearance:0.28||SGD DESC:Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol Gene:ARH1(YDR376W)|FD-Score:-3.54|P-value:2.03E-4|Clearance:0||SGD DESC:Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability Gene:GPN2(YOR262W)|FD-Score:-8.67|P-value:2.10E-18|Clearance:0||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:ISD11(YER048W-A)|FD-Score:-3.42|P-value:3.15E-4|Clearance:0||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:POL31(YJR006W)|FD-Score:3.12|P-value:9.11E-4|Clearance:0.03||SGD DESC:Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair Gene:RAP1(YNL216W)|FD-Score:-3.18|P-value:7.43E-4|Clearance:0||SGD DESC:Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) Gene:RPL17A(YKL180W)|FD-Score:6.8|P-value:5.29E-12|Clearance:3.36||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SHQ1(YIL104C)|FD-Score:-3.84|P-value:6.10E-5|Clearance:0||SGD DESC:Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones Gene:YLR140W(YLR140W_d)|FD-Score:-3.77|P-value:8.05E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I Gene:YPR136C(YPR136C_d)|FD-Score:3.15|P-value:8.11E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 Gene:ALG2(YGL065C)|FD-Score:3.43|P-value:2.97E-4|Clearance:0.28||SGD DESC:Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol Gene:ARH1(YDR376W)|FD-Score:-3.54|P-value:2.03E-4|Clearance:0||SGD DESC:Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability Gene:GPN2(YOR262W)|FD-Score:-8.67|P-value:2.10E-18|Clearance:0||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:ISD11(YER048W-A)|FD-Score:-3.42|P-value:3.15E-4|Clearance:0||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:POL31(YJR006W)|FD-Score:3.12|P-value:9.11E-4|Clearance:0.03||SGD DESC:Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair Gene:RAP1(YNL216W)|FD-Score:-3.18|P-value:7.43E-4|Clearance:0||SGD DESC:Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) Gene:RPL17A(YKL180W)|FD-Score:6.8|P-value:5.29E-12|Clearance:3.36||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SHQ1(YIL104C)|FD-Score:-3.84|P-value:6.10E-5|Clearance:0||SGD DESC:Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones Gene:YLR140W(YLR140W_d)|FD-Score:-3.77|P-value:8.05E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I Gene:YPR136C(YPR136C_d)|FD-Score:3.15|P-value:8.11E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17015612
Download HOP data (tab-delimited text)  (excel)
Gene:ALG9(YNL219C)|FD-Score:-4.26|P-value:1.03E-5||SGD DESC:Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation Gene:APN1(YKL114C)|FD-Score:4.71|P-value:1.24E-6||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ATG17(YLR423C)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:BAP2(YBR068C)|FD-Score:-3.57|P-value:1.79E-4||SGD DESC:High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication Gene:BBC1(YJL020C)|FD-Score:-3.23|P-value:6.29E-4||SGD DESC:Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches Gene:BTS1(YPL069C)|FD-Score:3.89|P-value:5.04E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:COG5(YNL051W)|FD-Score:-3.1|P-value:9.57E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTK3(YML112W)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DIT2(YDR402C)|FD-Score:-3.8|P-value:7.26E-5||SGD DESC:N-formyltyrosine oxidase, sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s Gene:EMC1(YCL045C)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090 Gene:ERP3(YDL018C)|FD-Score:-3.19|P-value:7.24E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FCY1(YPR062W)|FD-Score:-3.16|P-value:7.88E-4||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:FKS3(YMR306W)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FOX2(YKR009C)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities Gene:FRE6(YLL051C)|FD-Score:3.2|P-value:6.87E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels Gene:GGA1(YDR358W)|FD-Score:-4.36|P-value:6.54E-6||SGD DESC:Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Gene:GND2(YGR256W)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication Gene:GTR2(YGR163W)|FD-Score:-4.41|P-value:5.13E-6||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HCR1(YLR192C)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HEL1(YKR017C_p)|FD-Score:-3.78|P-value:7.70E-5||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU) Gene:IRC11(YOR013W_d)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Dubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci Gene:KCC4(YCL024W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Protein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication Gene:MIC23(YBL107C_p)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Mitochondrial intermembrane space protein of unknown function; imported via the MIA import machinery; contains an unusual twin cysteine motif (CX13C CX14C) Gene:MRPL22(YNL177C)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH1(YHR120W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:MSS116(YDR194C)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing Gene:NCE102(YPR149W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs) Gene:PDH1(YPR002W)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PHO80(YOL001W)|FD-Score:4.7|P-value:1.32E-6||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PPT2(YPL148C)|FD-Score:-3.76|P-value:8.45E-5||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PUS6(YGR169C)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:tRNA:pseudouridine synthase, catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability Gene:QCR2(YPR191W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Subunit 2 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; phosphorylated; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme Gene:QCR8(YJL166W)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p Gene:REC104(YHR157W)|FD-Score:5.18|P-value:1.14E-7||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:RGP1(YDR137W)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPA49(YNL248C)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:RNA polymerase I subunit A49 Gene:RPL18B(YNL301C)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication Gene:RPL23B(YER117W)|FD-Score:3.98|P-value:3.50E-5||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-4.71|P-value:1.23E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPP2A(YOL039W)|FD-Score:3.12|P-value:8.90E-4||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RPS12(YOR369C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog Gene:SAC3(YDR159W)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SIP18(YMR175W)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Phospholipid-binding hydrophilin; essential to overcome desiccation-rehydration process; expression is induced by osmotic stress Gene:SRB8(YCR081W)|FD-Score:6.94|P-value:1.91E-12||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SSO2(YMR183C)|FD-Score:4.05|P-value:2.53E-5||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p Gene:TDA8(YAL064C-A_p)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:VMA5(YKL080W)|FD-Score:-3.12|P-value:9.17E-4||SGD DESC:Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS3(YDR495C)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:YBR139W(YBR139W)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner Gene:YDL062W(YDL062W_d)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YDL063C; YDL062W is not essential Gene:YDL187C(YDL187C_d)|FD-Score:3.12|P-value:9.02E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR095C(YDR095C_d)|FD-Score:-3.23|P-value:6.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR249C(YDR249C_p)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Putative protein of unknown function Gene:YDR374C(YDR374C_p)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:Putative protein of unknown function Gene:YGL138C(YGL138C_p)|FD-Score:-4.02|P-value:2.94E-5||SGD DESC:Putative protein of unknown function; has no significant sequence similarity to any known protein Gene:YGP1(YNL160W)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; has similarity to Sps100p Gene:YGR139W(YGR139W_d)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR125W(YHR125W_d)|FD-Score:5.76|P-value:4.15E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR210C(YHR210C_p)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YKL077W(YKL077W_p)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YKL123W(YKL123W_d)|FD-Score:3.11|P-value:9.31E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene SSH4 Gene:YKR018C(YKR018C_p)|FD-Score:-3.75|P-value:8.98E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YLR046C(YLR046C_p)|FD-Score:-5.49|P-value:2.01E-8||SGD DESC:Putative membrane protein; member of the fungal lipid-translocating exporter (LTE) family of proteins; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:YLR053C(YLR053C_p)|FD-Score:-3.21|P-value:6.56E-4||SGD DESC:Putative protein of unknown function Gene:YMR196W(YMR196W_p)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YMR304C-A(YMR304C-A_d)|FD-Score:-3.21|P-value:6.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10 Gene:YNL089C(YNL089C_d)|FD-Score:-3.14|P-value:8.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins Gene:YOR015W(YOR015W_d)|FD-Score:4.93|P-value:4.19E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR019W(YOR019W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YPL038W-A(YPL038W-A_p)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPL080C(YPL080C_d)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL088W(YPL088W_p)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Putative aryl alcohol dehydrogenase; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:YPL229W(YPL229W_p)|FD-Score:3.86|P-value:5.62E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication Gene:ALG9(YNL219C)|FD-Score:-4.26|P-value:1.03E-5||SGD DESC:Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation Gene:APN1(YKL114C)|FD-Score:4.71|P-value:1.24E-6||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ATG17(YLR423C)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:BAP2(YBR068C)|FD-Score:-3.57|P-value:1.79E-4||SGD DESC:High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication Gene:BBC1(YJL020C)|FD-Score:-3.23|P-value:6.29E-4||SGD DESC:Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches Gene:BTS1(YPL069C)|FD-Score:3.89|P-value:5.04E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:COG5(YNL051W)|FD-Score:-3.1|P-value:9.57E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTK3(YML112W)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DIT2(YDR402C)|FD-Score:-3.8|P-value:7.26E-5||SGD DESC:N-formyltyrosine oxidase, sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s Gene:EMC1(YCL045C)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090 Gene:ERP3(YDL018C)|FD-Score:-3.19|P-value:7.24E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FCY1(YPR062W)|FD-Score:-3.16|P-value:7.88E-4||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:FKS3(YMR306W)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FOX2(YKR009C)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities Gene:FRE6(YLL051C)|FD-Score:3.2|P-value:6.87E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels Gene:GGA1(YDR358W)|FD-Score:-4.36|P-value:6.54E-6||SGD DESC:Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Gene:GND2(YGR256W)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication Gene:GTR2(YGR163W)|FD-Score:-4.41|P-value:5.13E-6||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HCR1(YLR192C)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HEL1(YKR017C_p)|FD-Score:-3.78|P-value:7.70E-5||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU) Gene:IRC11(YOR013W_d)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Dubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci Gene:KCC4(YCL024W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Protein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication Gene:MIC23(YBL107C_p)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Mitochondrial intermembrane space protein of unknown function; imported via the MIA import machinery; contains an unusual twin cysteine motif (CX13C CX14C) Gene:MRPL22(YNL177C)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH1(YHR120W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:MSS116(YDR194C)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing Gene:NCE102(YPR149W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs) Gene:PDH1(YPR002W)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PHO80(YOL001W)|FD-Score:4.7|P-value:1.32E-6||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PPT2(YPL148C)|FD-Score:-3.76|P-value:8.45E-5||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PUS6(YGR169C)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:tRNA:pseudouridine synthase, catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability Gene:QCR2(YPR191W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Subunit 2 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; phosphorylated; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme Gene:QCR8(YJL166W)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p Gene:REC104(YHR157W)|FD-Score:5.18|P-value:1.14E-7||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:RGP1(YDR137W)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPA49(YNL248C)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:RNA polymerase I subunit A49 Gene:RPL18B(YNL301C)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication Gene:RPL23B(YER117W)|FD-Score:3.98|P-value:3.50E-5||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-4.71|P-value:1.23E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPP2A(YOL039W)|FD-Score:3.12|P-value:8.90E-4||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RPS12(YOR369C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog Gene:SAC3(YDR159W)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SIP18(YMR175W)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Phospholipid-binding hydrophilin; essential to overcome desiccation-rehydration process; expression is induced by osmotic stress Gene:SRB8(YCR081W)|FD-Score:6.94|P-value:1.91E-12||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SSO2(YMR183C)|FD-Score:4.05|P-value:2.53E-5||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p Gene:TDA8(YAL064C-A_p)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:VMA5(YKL080W)|FD-Score:-3.12|P-value:9.17E-4||SGD DESC:Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS3(YDR495C)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:YBR139W(YBR139W)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner Gene:YDL062W(YDL062W_d)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YDL063C; YDL062W is not essential Gene:YDL187C(YDL187C_d)|FD-Score:3.12|P-value:9.02E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR095C(YDR095C_d)|FD-Score:-3.23|P-value:6.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR249C(YDR249C_p)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Putative protein of unknown function Gene:YDR374C(YDR374C_p)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:Putative protein of unknown function Gene:YGL138C(YGL138C_p)|FD-Score:-4.02|P-value:2.94E-5||SGD DESC:Putative protein of unknown function; has no significant sequence similarity to any known protein Gene:YGP1(YNL160W)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; has similarity to Sps100p Gene:YGR139W(YGR139W_d)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR125W(YHR125W_d)|FD-Score:5.76|P-value:4.15E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR210C(YHR210C_p)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YKL077W(YKL077W_p)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YKL123W(YKL123W_d)|FD-Score:3.11|P-value:9.31E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene SSH4 Gene:YKR018C(YKR018C_p)|FD-Score:-3.75|P-value:8.98E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YLR046C(YLR046C_p)|FD-Score:-5.49|P-value:2.01E-8||SGD DESC:Putative membrane protein; member of the fungal lipid-translocating exporter (LTE) family of proteins; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:YLR053C(YLR053C_p)|FD-Score:-3.21|P-value:6.56E-4||SGD DESC:Putative protein of unknown function Gene:YMR196W(YMR196W_p)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YMR304C-A(YMR304C-A_d)|FD-Score:-3.21|P-value:6.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10 Gene:YNL089C(YNL089C_d)|FD-Score:-3.14|P-value:8.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins Gene:YOR015W(YOR015W_d)|FD-Score:4.93|P-value:4.19E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR019W(YOR019W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YPL038W-A(YPL038W-A_p)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPL080C(YPL080C_d)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL088W(YPL088W_p)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Putative aryl alcohol dehydrogenase; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:YPL229W(YPL229W_p)|FD-Score:3.86|P-value:5.62E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL180W6.805.29E-123.36RPL17ARibosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
YGL065C3.432.97E-40.28ALG2Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol
YPR136C_d3.158.11E-40.03YPR136C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9
YJR006W3.129.11E-40.03POL31Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair
YER009W3.090.001020.06NTF2Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport
YLL050C3.020.001240.04COF1Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes
YCR054C2.990.001400.16CTR86Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress
YHR005C-A2.830.002350.01TIM10Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane
YDL140C2.820.002410.09RPO21RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
YBR243C2.730.003200.10ALG7UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
YOR157C2.630.004330.02PUP1Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z
YBL105C2.610.004590.07PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YDR160W2.530.005660.02SSY1Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes
YPR055W2.510.006050.02SEC8Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress
YMR033W2.490.006380.01ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YCR081W6.941.91E-12SRB8Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression
YHR125W_d5.764.15E-9YHR125W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YHR157W5.181.14E-7REC104Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination
YOR015W_d4.934.19E-7YOR015W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YKL114C4.711.24E-6APN1Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine
YOL001W4.701.32E-6PHO80Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling
YML112W4.231.18E-5CTK3Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress
YLR192C4.121.86E-5HCR1Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA
YAL064C-A_p4.092.14E-5TDA8_pPutative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene
YMR183C4.052.53E-5SSO2Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p
YNL248C4.052.55E-5RPA49RNA polymerase I subunit A49
YER117W3.983.50E-5RPL23BRibosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication
YPL069C3.895.04E-5BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YDR194C3.885.16E-5MSS116DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing
YPL229W_p3.865.62E-5YPL229W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication

GO enrichment analysis for SGTC_3291
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0672.80E-7SGTC_7273032-0951 202.0 μMChemDiv (Drug-like library)36528360.1375
0.0663.71E-7SGTC_22216807166 181.1 μMChembridge (Fragment library)6749250.0875
0.0621.83E-6SGTC_1764st044502 93.5 μMTimTec (Natural product derivative library)84070.0857143
0.0603.74E-6SGTC_30949117742 49.5 μMChembridge (Drug-like library)171496980.148148
0.0588.04E-6SGTC_2665lovastatin 100.0 μMMiscellaneous532320.0673077
0.0571.36E-5SGTC_32839109332 35.8 μMChembridge (Drug-like library)170272810.253165DNA intercalators
0.0552.33E-5SGTC_22016605497 169.9 μMChembridge (Fragment library)6707410.140845ergosterol biosynthesis
0.0552.67E-5SGTC_478N-arachidonylglycine 10.0 μMICCB bioactive library52833890.0909091
0.0543.26E-5SGTC_8324083-0019 164.0 μMChemDiv (Drug-like library)19907580.115789translation
0.0533.91E-5SGTC_28889058547 13.0 μMChembridge (Drug-like library)164765140.171053
0.0533.96E-5SGTC_418lividomycin a 100.0 μMMiscellaneous723940.01
0.0525.80E-5SGTC_9443370-0224 231.0 μMChemDiv (Drug-like library)45805140.133333
0.0526.94E-5SGTC_13903413-2614 145.0 μMChemDiv (Drug-like library)34214320.0128205fatty acid desaturase (OLE1)
0.0517.64E-5SGTC_3214534-3890 65.3 μMChemDiv (Drug-like library)6997460.2375RPP1 & pyrimidine depletion
0.0511.01E-4SGTC_1965Pectolinarigenin 58.4 μMTimTec (Natural product derivative library)53204380.0705882RPP1 & pyrimidine depletion

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3289911991271.43 μM0.517014829Chembridge (Drug-like library)384.816283.88415
SGTC_22406690706185.95 μM0.409091805876Chembridge (Fragment library)266.294562.55213
SGTC_21195528445120.64 μM0.383333791935Chembridge (Fragment library)212.290243.04412
SGTC_2934903637036.88 μM0.3756462800Chembridge (Drug-like library)348.810623.01316
SGTC_3209911361649.47 μM0.37516649353Chembridge (Drug-like library)342.77963.59514
SGTC_2976900900519.48 μM0.3731342993148Chembridge (Drug-like library)299.390683.28723
SGTC_3325913876055.18 μM0.36486543844959Chembridge (Drug-like library)356.806183.38524Golgi
SGTC_3201911399949.47 μM0.36231916649224Chembridge (Drug-like library)336.38773.90314
SGTC_2972909361959.92 μM0.3625236256Chembridge (Drug-like library)300.41853.51513
SGTC_3342914779114.51 μM0.3513511838168Chembridge (Drug-like library)349.816843.34914