9120825

6-(2-fluorophenyl)-3-(2-methylpropyl)-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3292
Screen concentration 59.8 μM
Source Chembridge (Drug-like library)
PubChem CID 24824900
SMILES CC(C)CC1=NN=C2N1N=C(S2)C3=CC=CC=C3F
Standardized SMILES CC(C)Cc1nnc2sc(nn12)c3ccccc3F
Molecular weight 276.3325
ALogP 3.29
H-bond donor count 0
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.7
% growth inhibition (Hom. pool) 15.37


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 24824900
Download HIP data (tab-delimited text)  (excel)
Gene:ALG14(YBR070C)|FD-Score:-3.37|P-value:3.72E-4|Clearance:0||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:CBF2(YGR140W)|FD-Score:3.73|P-value:9.51E-5|Clearance:0.08||SGD DESC:Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress Gene:CDC31(YOR257W)|FD-Score:-3.24|P-value:5.99E-4|Clearance:0||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:GCD1(YOR260W)|FD-Score:-3.54|P-value:1.99E-4|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:ISD11(YER048W-A)|FD-Score:-3.35|P-value:4.10E-4|Clearance:0||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:KRE29(YER038C)|FD-Score:3.39|P-value:3.53E-4|Clearance:0.16||SGD DESC:Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:MEX67(YPL169C)|FD-Score:4.99|P-value:2.95E-7|Clearance:0.98||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:PFY1(YOR122C)|FD-Score:-3.43|P-value:3.03E-4|Clearance:0||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:PRI1(YIR008C)|FD-Score:3.23|P-value:6.17E-4|Clearance:0.19||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:PRP4(YPR178W)|FD-Score:3.45|P-value:2.83E-4|Clearance:0.06||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPA43(YOR340C)|FD-Score:-6.01|P-value:9.17E-10|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:TID3(YIL144W)|FD-Score:-3.79|P-value:7.40E-5|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:TIF11(YMR260C)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.21||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 Gene:TUB1(YML085C)|FD-Score:-3.38|P-value:3.67E-4|Clearance:0||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YSH1(YLR277C)|FD-Score:4.02|P-value:2.96E-5|Clearance:0.28||SGD DESC:Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication Gene:ALG14(YBR070C)|FD-Score:-3.37|P-value:3.72E-4|Clearance:0||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:CBF2(YGR140W)|FD-Score:3.73|P-value:9.51E-5|Clearance:0.08||SGD DESC:Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress Gene:CDC31(YOR257W)|FD-Score:-3.24|P-value:5.99E-4|Clearance:0||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:GCD1(YOR260W)|FD-Score:-3.54|P-value:1.99E-4|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:ISD11(YER048W-A)|FD-Score:-3.35|P-value:4.10E-4|Clearance:0||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:KRE29(YER038C)|FD-Score:3.39|P-value:3.53E-4|Clearance:0.16||SGD DESC:Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:MEX67(YPL169C)|FD-Score:4.99|P-value:2.95E-7|Clearance:0.98||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:PFY1(YOR122C)|FD-Score:-3.43|P-value:3.03E-4|Clearance:0||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:PRI1(YIR008C)|FD-Score:3.23|P-value:6.17E-4|Clearance:0.19||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:PRP4(YPR178W)|FD-Score:3.45|P-value:2.83E-4|Clearance:0.06||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPA43(YOR340C)|FD-Score:-6.01|P-value:9.17E-10|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:TID3(YIL144W)|FD-Score:-3.79|P-value:7.40E-5|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:TIF11(YMR260C)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.21||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 Gene:TUB1(YML085C)|FD-Score:-3.38|P-value:3.67E-4|Clearance:0||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YSH1(YLR277C)|FD-Score:4.02|P-value:2.96E-5|Clearance:0.28||SGD DESC:Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 24824900
Download HOP data (tab-delimited text)  (excel)
Gene:ADO1(YJR105W)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AIM17(YHL021C)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:ALG9(YNL219C)|FD-Score:-3.3|P-value:4.75E-4||SGD DESC:Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation Gene:ARG80(YMR042W)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Transcription factor involved in regulation of arginine-responsive genes; acts with Arg81p and Arg82p Gene:ARO1(YDR127W)|FD-Score:5.86|P-value:2.35E-9||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATP17(YDR377W)|FD-Score:3.27|P-value:5.28E-4||SGD DESC:Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:AVO2(YMR068W)|FD-Score:-4.27|P-value:9.70E-6||SGD DESC:Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:AYR1(YIL124W)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones Gene:BDS1(YOL164W)|FD-Score:-3.93|P-value:4.30E-5||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:BRE1(YDL074C)|FD-Score:-4.19|P-value:1.40E-5||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:BTS1(YPL069C)|FD-Score:-3.37|P-value:3.78E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CNM67(YNL225C)|FD-Score:-3.21|P-value:6.55E-4||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COA3(YJL062W-A)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Mitochondrial inner membrane protein that participates in regulation of COX1 translation, Cox1p stabilization, and cytochrome oxidase assembly Gene:COS111(YBR203W)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DAL81(YIR023W)|FD-Score:6.09|P-value:5.69E-10||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DDC1(YPL194W)|FD-Score:4.28|P-value:9.54E-6||SGD DESC:DNA damage checkpoint protein; part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate; forms nuclear foci upon DNA replication stress Gene:DUG2(YBR281C)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:GET2(YER083C)|FD-Score:4.46|P-value:4.04E-6||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GSH1(YJL101C)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress Gene:HCR1(YLR192C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HIS3(YOR202W)|FD-Score:3.82|P-value:6.76E-5||SGD DESC:Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p Gene:IRA2(YOL081W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication Gene:IRS4(YKR019C)|FD-Score:8.73|P-value:1.26E-18||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KIN1(YDR122W)|FD-Score:4.44|P-value:4.42E-6||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p Gene:MBP1(YDL056W)|FD-Score:4.29|P-value:8.76E-6||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MFA2(YNL145W)|FD-Score:3.77|P-value:8.04E-5||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1 Gene:MIR1(YJR077C)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated Gene:MOH1(YBL049W)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MRPS9(YBR146W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MTC2(YKL098W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:NUP60(YAR002W)|FD-Score:-3.33|P-value:4.29E-4||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:PPH21(YDL134C)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication Gene:PUS4(YNL292W)|FD-Score:3.98|P-value:3.38E-5||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) Gene:RAD2(YGR258C)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein Gene:RAM1(YDL090C)|FD-Score:-4.03|P-value:2.78E-5||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RCE1(YMR274C)|FD-Score:5.59|P-value:1.11E-8||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RFX1(YLR176C)|FD-Score:4.98|P-value:3.24E-7||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:RIM21(YNL294C)|FD-Score:-3.36|P-value:3.92E-4||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPN4(YDL020C)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SDT1(YGL224C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives Gene:SEY1(YOR165W)|FD-Score:6.91|P-value:2.34E-12||SGD DESC:Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 Gene:SFT2(YBL102W)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 Gene:TAT1(YBR069C)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:VPS45(YGL095C)|FD-Score:4.77|P-value:9.06E-7||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YDL034W(YDL034W_d)|FD-Score:3.15|P-value:8.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene Gene:YDL071C(YDL071C_d)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W Gene:YDR193W(YDR193W_d)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR290W(YDR290W_d)|FD-Score:-3.86|P-value:5.77E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YER175W-A(YER175W-A_p)|FD-Score:3.72|P-value:9.99E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YFT2(YDR319C_p)|FD-Score:-3.83|P-value:6.28E-5||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YIL060W(YIL060W_p)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YIL100W(YIL100W_d)|FD-Score:-3.28|P-value:5.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the dubious ORF YIL100C-A Gene:YJL127C-B(YJL127C-B_p)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Putative protein of unknown function; identified based on homology to the filamentous fungus, <i>Ashbya gossypii</i> Gene:YJL213W(YJL213W)|FD-Score:-3.19|P-value:7.17E-4||SGD DESC:Protein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p Gene:YLR202C(YLR202C_d)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YMR196W(YMR196W_p)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YNL217W(YNL217W_p)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Putative protein of unknown function; weak sequence similarity to bis (5'-nucleotidyl)-tetraphosphatases; (GFP)-fusion protein localizes to the vacuole; null mutant is highly sensitive to azaserine and resistant to sodium-O-vandate Gene:YPL088W(YPL088W_p)|FD-Score:-4.65|P-value:1.68E-6||SGD DESC:Putative aryl alcohol dehydrogenase; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:ADO1(YJR105W)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AIM17(YHL021C)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:ALG9(YNL219C)|FD-Score:-3.3|P-value:4.75E-4||SGD DESC:Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation Gene:ARG80(YMR042W)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Transcription factor involved in regulation of arginine-responsive genes; acts with Arg81p and Arg82p Gene:ARO1(YDR127W)|FD-Score:5.86|P-value:2.35E-9||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATP17(YDR377W)|FD-Score:3.27|P-value:5.28E-4||SGD DESC:Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:AVO2(YMR068W)|FD-Score:-4.27|P-value:9.70E-6||SGD DESC:Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:AYR1(YIL124W)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones Gene:BDS1(YOL164W)|FD-Score:-3.93|P-value:4.30E-5||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:BRE1(YDL074C)|FD-Score:-4.19|P-value:1.40E-5||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:BTS1(YPL069C)|FD-Score:-3.37|P-value:3.78E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CNM67(YNL225C)|FD-Score:-3.21|P-value:6.55E-4||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COA3(YJL062W-A)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Mitochondrial inner membrane protein that participates in regulation of COX1 translation, Cox1p stabilization, and cytochrome oxidase assembly Gene:COS111(YBR203W)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DAL81(YIR023W)|FD-Score:6.09|P-value:5.69E-10||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DDC1(YPL194W)|FD-Score:4.28|P-value:9.54E-6||SGD DESC:DNA damage checkpoint protein; part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate; forms nuclear foci upon DNA replication stress Gene:DUG2(YBR281C)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:GET2(YER083C)|FD-Score:4.46|P-value:4.04E-6||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GSH1(YJL101C)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress Gene:HCR1(YLR192C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HIS3(YOR202W)|FD-Score:3.82|P-value:6.76E-5||SGD DESC:Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p Gene:IRA2(YOL081W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication Gene:IRS4(YKR019C)|FD-Score:8.73|P-value:1.26E-18||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KIN1(YDR122W)|FD-Score:4.44|P-value:4.42E-6||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p Gene:MBP1(YDL056W)|FD-Score:4.29|P-value:8.76E-6||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MFA2(YNL145W)|FD-Score:3.77|P-value:8.04E-5||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1 Gene:MIR1(YJR077C)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated Gene:MOH1(YBL049W)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MRPS9(YBR146W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MTC2(YKL098W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:NUP60(YAR002W)|FD-Score:-3.33|P-value:4.29E-4||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:PPH21(YDL134C)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication Gene:PUS4(YNL292W)|FD-Score:3.98|P-value:3.38E-5||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) Gene:RAD2(YGR258C)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein Gene:RAM1(YDL090C)|FD-Score:-4.03|P-value:2.78E-5||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RCE1(YMR274C)|FD-Score:5.59|P-value:1.11E-8||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RFX1(YLR176C)|FD-Score:4.98|P-value:3.24E-7||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:RIM21(YNL294C)|FD-Score:-3.36|P-value:3.92E-4||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPN4(YDL020C)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SDT1(YGL224C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives Gene:SEY1(YOR165W)|FD-Score:6.91|P-value:2.34E-12||SGD DESC:Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 Gene:SFT2(YBL102W)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 Gene:TAT1(YBR069C)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:VPS45(YGL095C)|FD-Score:4.77|P-value:9.06E-7||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YDL034W(YDL034W_d)|FD-Score:3.15|P-value:8.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene Gene:YDL071C(YDL071C_d)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W Gene:YDR193W(YDR193W_d)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR290W(YDR290W_d)|FD-Score:-3.86|P-value:5.77E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YER175W-A(YER175W-A_p)|FD-Score:3.72|P-value:9.99E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YFT2(YDR319C_p)|FD-Score:-3.83|P-value:6.28E-5||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YIL060W(YIL060W_p)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YIL100W(YIL100W_d)|FD-Score:-3.28|P-value:5.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the dubious ORF YIL100C-A Gene:YJL127C-B(YJL127C-B_p)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Putative protein of unknown function; identified based on homology to the filamentous fungus, <i>Ashbya gossypii</i> Gene:YJL213W(YJL213W)|FD-Score:-3.19|P-value:7.17E-4||SGD DESC:Protein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p Gene:YLR202C(YLR202C_d)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YMR196W(YMR196W_p)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YNL217W(YNL217W_p)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Putative protein of unknown function; weak sequence similarity to bis (5'-nucleotidyl)-tetraphosphatases; (GFP)-fusion protein localizes to the vacuole; null mutant is highly sensitive to azaserine and resistant to sodium-O-vandate Gene:YPL088W(YPL088W_p)|FD-Score:-4.65|P-value:1.68E-6||SGD DESC:Putative aryl alcohol dehydrogenase; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL169C4.992.95E-70.98MEX67Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
YLR277C4.022.96E-50.28YSH1Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication
YGR140W3.739.51E-50.08CBF2Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress
YMR260C3.651.29E-40.21TIF11Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3
YPR178W3.452.83E-40.06PRP4Splicing factor, component of the U4/U6-U5 snRNP complex
YER038C3.393.53E-40.16KRE29Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
YIR008C3.236.17E-40.19PRI1Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
YMR314W3.040.001190.03PRE5Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
YDL031W3.000.001330.03DBP10Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis
YLR321C2.980.001450.10SFH1Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog
YBR002C2.880.002000.04RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YFL022C2.830.002300.03FRS2Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
YJL143W2.810.002500.10TIM17Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex
YNR017W2.710.003410.01TIM23Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel
YBR254C2.700.003470.05TRS20One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKR019C8.731.26E-18IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YOR165W6.912.34E-12SEY1Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3
YIR023W6.095.69E-10DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YDR127W5.862.35E-9ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YMR274C5.591.11E-8RCE1Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone
YLR176C4.983.24E-7RFX1Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins
YGL095C4.779.06E-7VPS45Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
YER083C4.464.04E-6GET2Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division
YDR122W4.444.42E-6KIN1Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p
YDL056W4.298.76E-6MBP1Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes
YPL194W4.289.54E-6DDC1DNA damage checkpoint protein; part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate; forms nuclear foci upon DNA replication stress
YNL292W3.983.38E-5PUS4Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype)
YOR202W3.826.76E-5HIS3Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p
YNL145W3.778.04E-5MFA2Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1
YER175W-A_p3.729.99E-5YER175W-A_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching

GO enrichment analysis for SGTC_3292
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1843.10E-46SGTC_486niguldipine 82.0 μMMiscellaneous12360.0714286amide catabolism
0.1845.67E-46SGTC_6051315-0399 119.0 μMChemDiv (Drug-like library)240204610.075Golgi
0.1721.26E-40SGTC_14780453-0656 35.7 μMChemDiv (Drug-like library)52584960.0555556
0.1651.77E-37SGTC_24605552655 174.2 μMMiscellaneous54019890.0869565amide catabolism
0.1581.71E-34SGTC_14740330-0125 5.3 μMChemDiv (Drug-like library)X14740.0666667
0.1534.45E-32SGTC_9332914-0560 114.0 μMChemDiv (Drug-like library)4638870.112903
0.1521.02E-31SGTC_22557941087 197.4 μMChembridge (Fragment library)9355610.1
0.1462.06E-29SGTC_3371315-0038 354.0 μMChemDiv (Drug-like library)16264370.0987654amide catabolism
0.1425.31E-28SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0697674
0.1368.95E-26SGTC_13131220-0047 237.0 μMChemDiv (Drug-like library)35287060.0526316
0.1344.11E-25SGTC_10583448-5425 97.3 μMChemDiv (Drug-like library)72797050.109375
0.1331.29E-24SGTC_31019120531 49.5 μMChembridge (Drug-like library)248249040.360656
0.1291.76E-23SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.0882353
0.1284.34E-23SGTC_413propylparaben 100.0 μMMiscellaneous71750.0833333amide catabolism
0.1262.05E-22SGTC_12790874-0590 65.2 μMChemDiv (Drug-like library)35230740.0428571

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3102912166949.47 μM0.7541905072Chembridge (Drug-like library)272.368643.57504
SGTC_3306912767659.79 μM0.60416741905287Chembridge (Drug-like library)264.369783.04205
SGTC_3073911497249.47 μM0.5841905075Chembridge (Drug-like library)272.368643.5750460S ribosome export
SGTC_3300912461550.48 μM0.5624824903Chembridge (Drug-like library)288.368043.07205
SGTC_3299912198243.13 μM0.5490217594604Chembridge (Drug-like library)273.35672.22105
SGTC_3301912535419.8 μM0.5471741905162Chembridge (Drug-like library)301.409863.25105
SGTC_3302912539516.35 μM0.52941217599668Chembridge (Drug-like library)284.379343.55704
SGTC_3116912494349.47 μM0.51923141905195Chembridge (Drug-like library)288.368042.92405
SGTC_3305912702753.83 μM0.50909141905295Chembridge (Drug-like library)316.378142.87506
SGTC_3307912688569.28 μM0.524824909Chembridge (Drug-like library)272.368643.77904