9120939

6-(2-methoxyphenyl)-3-pyridin-2-yl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3293
Screen concentration 62.0 μM
Source Chembridge (Drug-like library)
PubChem CID 934087
SMILES COC1=CC=CC=C1C2=NN3C(=NN=C3S2)C4=CC=CC=N4
Standardized SMILES COc1ccccc1c2nn3c(nnc3s2)c4ccccn4
Molecular weight 309.3457
ALogP 2.64
H-bond donor count 0
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.57
% growth inhibition (Hom. pool) 10.46


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 934087
Download HIP data (tab-delimited text)  (excel)
Gene:ALG7(YBR243C)|FD-Score:4.04|P-value:2.71E-5|Clearance:0.03||SGD DESC:UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin Gene:ARB1(YER036C)|FD-Score:3.31|P-value:4.70E-4|Clearance:0.09||SGD DESC:ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p Gene:ARP4(YJL081C)|FD-Score:3.48|P-value:2.48E-4|Clearance:0||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ATP16(YDL004W)|FD-Score:-3.61|P-value:1.56E-4|Clearance:0||SGD DESC:Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BMS1(YPL217C)|FD-Score:3.14|P-value:8.52E-4|Clearance:0.06||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CBF2(YGR140W)|FD-Score:4.01|P-value:3.04E-5|Clearance:0.4||SGD DESC:Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress Gene:CDC24(YAL041W)|FD-Score:3.61|P-value:1.50E-4|Clearance:0.07||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:DAD1(YDR016C)|FD-Score:3.55|P-value:1.94E-4|Clearance:0.06||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DRE2(YKR071C)|FD-Score:4.47|P-value:3.97E-6|Clearance:0.25||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:IRR1(YIL026C)|FD-Score:3.48|P-value:2.50E-4|Clearance:0.03||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:MES1(YGR264C)|FD-Score:3.34|P-value:4.17E-4|Clearance:0.03||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:MTR10(YOR160W)|FD-Score:3.22|P-value:6.41E-4|Clearance:0.02||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:PUP1(YOR157C)|FD-Score:4.73|P-value:1.14E-6|Clearance:0.09||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RFT1(YBL020W)|FD-Score:5.2|P-value:1.01E-7|Clearance:0.47||SGD DESC:Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein Gene:RPB10(YOR210W)|FD-Score:3.16|P-value:7.97E-4|Clearance:0.02||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPC17(YJL011C)|FD-Score:4.64|P-value:1.78E-6|Clearance:0.01||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPS15(YOL040C)|FD-Score:-4.48|P-value:3.66E-6|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RSC8(YFR037C)|FD-Score:4.57|P-value:2.47E-6|Clearance:0.1||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SEC7(YDR170C)|FD-Score:-5.05|P-value:2.22E-7|Clearance:0||SGD DESC:Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles Gene:SRP14(YDL092W)|FD-Score:3.45|P-value:2.78E-4|Clearance:0.11||SGD DESC:Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 Gene:SWI1(YPL016W)|FD-Score:-3.82|P-value:6.75E-5|Clearance:0||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer Gene:TAF6(YGL112C)|FD-Score:6.44|P-value:5.93E-11|Clearance:1.24||SGD DESC:Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 Gene:TFC3(YAL001C)|FD-Score:4.22|P-value:1.25E-5|Clearance:0.18||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:YGR265W(YGR265W_d)|FD-Score:3.2|P-value:6.76E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:YLL037W(YLL037W_d)|FD-Score:4.63|P-value:1.84E-6|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor Gene:ALG7(YBR243C)|FD-Score:4.04|P-value:2.71E-5|Clearance:0.03||SGD DESC:UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin Gene:ARB1(YER036C)|FD-Score:3.31|P-value:4.70E-4|Clearance:0.09||SGD DESC:ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p Gene:ARP4(YJL081C)|FD-Score:3.48|P-value:2.48E-4|Clearance:0||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ATP16(YDL004W)|FD-Score:-3.61|P-value:1.56E-4|Clearance:0||SGD DESC:Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BMS1(YPL217C)|FD-Score:3.14|P-value:8.52E-4|Clearance:0.06||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CBF2(YGR140W)|FD-Score:4.01|P-value:3.04E-5|Clearance:0.4||SGD DESC:Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress Gene:CDC24(YAL041W)|FD-Score:3.61|P-value:1.50E-4|Clearance:0.07||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:DAD1(YDR016C)|FD-Score:3.55|P-value:1.94E-4|Clearance:0.06||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DRE2(YKR071C)|FD-Score:4.47|P-value:3.97E-6|Clearance:0.25||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:IRR1(YIL026C)|FD-Score:3.48|P-value:2.50E-4|Clearance:0.03||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:MES1(YGR264C)|FD-Score:3.34|P-value:4.17E-4|Clearance:0.03||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:MTR10(YOR160W)|FD-Score:3.22|P-value:6.41E-4|Clearance:0.02||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:PUP1(YOR157C)|FD-Score:4.73|P-value:1.14E-6|Clearance:0.09||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RFT1(YBL020W)|FD-Score:5.2|P-value:1.01E-7|Clearance:0.47||SGD DESC:Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein Gene:RPB10(YOR210W)|FD-Score:3.16|P-value:7.97E-4|Clearance:0.02||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPC17(YJL011C)|FD-Score:4.64|P-value:1.78E-6|Clearance:0.01||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPS15(YOL040C)|FD-Score:-4.48|P-value:3.66E-6|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RSC8(YFR037C)|FD-Score:4.57|P-value:2.47E-6|Clearance:0.1||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SEC7(YDR170C)|FD-Score:-5.05|P-value:2.22E-7|Clearance:0||SGD DESC:Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles Gene:SRP14(YDL092W)|FD-Score:3.45|P-value:2.78E-4|Clearance:0.11||SGD DESC:Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 Gene:SWI1(YPL016W)|FD-Score:-3.82|P-value:6.75E-5|Clearance:0||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer Gene:TAF6(YGL112C)|FD-Score:6.44|P-value:5.93E-11|Clearance:1.24||SGD DESC:Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 Gene:TFC3(YAL001C)|FD-Score:4.22|P-value:1.25E-5|Clearance:0.18||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:YGR265W(YGR265W_d)|FD-Score:3.2|P-value:6.76E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:YLL037W(YLL037W_d)|FD-Score:4.63|P-value:1.84E-6|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 934087
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:-3.4|P-value:3.35E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM4(YBR194W)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress Gene:APM1(YPL259C)|FD-Score:5.19|P-value:1.04E-7||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:ARR3(YPR201W)|FD-Score:-3.91|P-value:4.56E-5||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:AST1(YBL069W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication Gene:ATG23(YLR431C)|FD-Score:-3.17|P-value:7.75E-4||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:BCK1(YJL095W)|FD-Score:5.89|P-value:1.94E-9||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CLB1(YGR108W)|FD-Score:5.14|P-value:1.38E-7||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:COA3(YJL062W-A)|FD-Score:3.27|P-value:5.41E-4||SGD DESC:Mitochondrial inner membrane protein that participates in regulation of COX1 translation, Cox1p stabilization, and cytochrome oxidase assembly Gene:COG7(YGL005C)|FD-Score:-4.1|P-value:2.08E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX20(YDR231C)|FD-Score:-3.42|P-value:3.10E-4||SGD DESC:Mitochondrial inner membrane protein, required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase Gene:COY1(YKL179C)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function Gene:CYC1(YJR048W)|FD-Score:4.4|P-value:5.32E-6||SGD DESC:Cytochrome c, isoform 1; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; CYC1 has a paralog, CYC7, that arose from the whole genome duplication Gene:DAL81(YIR023W)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DSE2(YHR143W)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:ERI1(YPL096C-A)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:FIG4(YNL325C)|FD-Score:4.36|P-value:6.55E-6||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:FOX2(YKR009C)|FD-Score:3.97|P-value:3.63E-5||SGD DESC:Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities Gene:GCY1(YOR120W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GSH1(YJL101C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress Gene:GTT2(YLL060C)|FD-Score:4.02|P-value:2.90E-5||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:IMP2'(YIL154C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:INO1(YJL153C)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element Gene:KIN1(YDR122W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p Gene:KKQ8(YKL168C)|FD-Score:3.13|P-value:8.86E-4||SGD DESC:Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication Gene:LDB19(YOR322C)|FD-Score:4.59|P-value:2.21E-6||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:MCT1(YOR221C)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MDS3(YGL197W)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MOD5(YOR274W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MRPL25(YGR076C)|FD-Score:-3.77|P-value:8.16E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan Gene:MTC7(YEL033W_p)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:MUP3(YHL036W)|FD-Score:-5.11|P-value:1.64E-7||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:ORM2(YLR350W)|FD-Score:3.95|P-value:3.85E-5||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:ORT1(YOR130C)|FD-Score:3.85|P-value:5.80E-5||SGD DESC:Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome Gene:PEX19(YDL065C)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning Gene:PHO80(YOL001W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:RDH54(YBR073W)|FD-Score:4.48|P-value:3.67E-6||SGD DESC:DNA-dependent ATPase; stimulates strand exchange by modifying the topology of double-stranded DNA; involved in recombinational repair of DNA double-strand breaks during mitosis and meiosis; proposed to be involved in crossover interference; interacts with Dmc1p; stimulates Dmc1p and Rad51p Gene:RIM9(YMR063W)|FD-Score:-4.14|P-value:1.74E-5||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:SEA4(YBL104C)|FD-Score:11.9|P-value:3.50E-33||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SHM2(YLR058C)|FD-Score:-3.18|P-value:7.39E-4||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SIP4(YJL089W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SOH1(YGL127C)|FD-Score:4.36|P-value:6.44E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SRB8(YCR081W)|FD-Score:-3.94|P-value:4.13E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:STE24(YJR117W)|FD-Score:5.03|P-value:2.40E-7||SGD DESC:Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans Gene:SWI3(YJL176C)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:TPO4(YOR273C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRI1(YMR233W)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TRP3(YKL211C)|FD-Score:-3.35|P-value:4.11E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UFD2(YDL190C)|FD-Score:-4.08|P-value:2.25E-5||SGD DESC:Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 Gene:VAN1(YML115C)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:VID22(YLR373C)|FD-Score:3.11|P-value:9.26E-4||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:VMA1(YDL185W)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA13(YPR036W)|FD-Score:-3.75|P-value:8.95E-5||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPH2(YKL119C)|FD-Score:-6.62|P-value:1.84E-11||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:VPS3(YDR495C)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS45(YGL095C)|FD-Score:-7.51|P-value:2.99E-14||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YAP5(YIR018W)|FD-Score:-3.1|P-value:9.72E-4||SGD DESC:Basic leucine zipper (bZIP) iron-sensing transcription factor; YAP5 has a paralog, YAP7, that arose from the whole genome duplication Gene:YBR196C-A(YBR196C-A_p)|FD-Score:-13|P-value:5.01E-39||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR242W(YBR242W_p)|FD-Score:4.19|P-value:1.38E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YDL034W(YDL034W_d)|FD-Score:4.48|P-value:3.79E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene Gene:YDR290W(YDR290W_d)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YER038W-A(YER038W-A_d)|FD-Score:4.53|P-value:2.98E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria Gene:YHR127W(YHR127W)|FD-Score:-3.85|P-value:5.90E-5||SGD DESC:Protein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis Gene:YKL147C(YKL147C_d)|FD-Score:4.36|P-value:6.43E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene AVT3 Gene:YKL162C(YKL162C_p)|FD-Score:3.73|P-value:9.46E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:YLR225C(YLR225C_p)|FD-Score:4.31|P-value:7.99E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene Gene:YLR312C(YLR312C_p)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Putative protein of unknown function Gene:YLR444C(YLR444C_d)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR245W(YMR245W_d)|FD-Score:-3.78|P-value:7.75E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR005C(YNR005C_d)|FD-Score:-3.98|P-value:3.45E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR071C(YNR071C_p)|FD-Score:4.17|P-value:1.53E-5||SGD DESC:Putative aldose 1-epimerase Gene:YOR318C(YOR318C_d)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo Gene:YOR343C(YOR343C_d)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL080C(YPL080C_d)|FD-Score:-5.97|P-value:1.20E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YSW1(YBR148W)|FD-Score:3.49|P-value:2.37E-4||SGD DESC:Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane Gene:AFT1(YGL071W)|FD-Score:-3.4|P-value:3.35E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM4(YBR194W)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress Gene:APM1(YPL259C)|FD-Score:5.19|P-value:1.04E-7||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:ARR3(YPR201W)|FD-Score:-3.91|P-value:4.56E-5||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:AST1(YBL069W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication Gene:ATG23(YLR431C)|FD-Score:-3.17|P-value:7.75E-4||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:BCK1(YJL095W)|FD-Score:5.89|P-value:1.94E-9||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CLB1(YGR108W)|FD-Score:5.14|P-value:1.38E-7||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:COA3(YJL062W-A)|FD-Score:3.27|P-value:5.41E-4||SGD DESC:Mitochondrial inner membrane protein that participates in regulation of COX1 translation, Cox1p stabilization, and cytochrome oxidase assembly Gene:COG7(YGL005C)|FD-Score:-4.1|P-value:2.08E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX20(YDR231C)|FD-Score:-3.42|P-value:3.10E-4||SGD DESC:Mitochondrial inner membrane protein, required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase Gene:COY1(YKL179C)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function Gene:CYC1(YJR048W)|FD-Score:4.4|P-value:5.32E-6||SGD DESC:Cytochrome c, isoform 1; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; CYC1 has a paralog, CYC7, that arose from the whole genome duplication Gene:DAL81(YIR023W)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DSE2(YHR143W)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:ERI1(YPL096C-A)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:FIG4(YNL325C)|FD-Score:4.36|P-value:6.55E-6||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:FOX2(YKR009C)|FD-Score:3.97|P-value:3.63E-5||SGD DESC:Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities Gene:GCY1(YOR120W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GSH1(YJL101C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress Gene:GTT2(YLL060C)|FD-Score:4.02|P-value:2.90E-5||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:IMP2'(YIL154C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:INO1(YJL153C)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element Gene:KIN1(YDR122W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p Gene:KKQ8(YKL168C)|FD-Score:3.13|P-value:8.86E-4||SGD DESC:Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication Gene:LDB19(YOR322C)|FD-Score:4.59|P-value:2.21E-6||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:MCT1(YOR221C)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MDS3(YGL197W)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MOD5(YOR274W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MRPL25(YGR076C)|FD-Score:-3.77|P-value:8.16E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan Gene:MTC7(YEL033W_p)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:MUP3(YHL036W)|FD-Score:-5.11|P-value:1.64E-7||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:ORM2(YLR350W)|FD-Score:3.95|P-value:3.85E-5||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:ORT1(YOR130C)|FD-Score:3.85|P-value:5.80E-5||SGD DESC:Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome Gene:PEX19(YDL065C)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning Gene:PHO80(YOL001W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:RDH54(YBR073W)|FD-Score:4.48|P-value:3.67E-6||SGD DESC:DNA-dependent ATPase; stimulates strand exchange by modifying the topology of double-stranded DNA; involved in recombinational repair of DNA double-strand breaks during mitosis and meiosis; proposed to be involved in crossover interference; interacts with Dmc1p; stimulates Dmc1p and Rad51p Gene:RIM9(YMR063W)|FD-Score:-4.14|P-value:1.74E-5||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:SEA4(YBL104C)|FD-Score:11.9|P-value:3.50E-33||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SHM2(YLR058C)|FD-Score:-3.18|P-value:7.39E-4||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SIP4(YJL089W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SOH1(YGL127C)|FD-Score:4.36|P-value:6.44E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SRB8(YCR081W)|FD-Score:-3.94|P-value:4.13E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:STE24(YJR117W)|FD-Score:5.03|P-value:2.40E-7||SGD DESC:Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans Gene:SWI3(YJL176C)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:TPO4(YOR273C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRI1(YMR233W)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TRP3(YKL211C)|FD-Score:-3.35|P-value:4.11E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UFD2(YDL190C)|FD-Score:-4.08|P-value:2.25E-5||SGD DESC:Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 Gene:VAN1(YML115C)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:VID22(YLR373C)|FD-Score:3.11|P-value:9.26E-4||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:VMA1(YDL185W)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA13(YPR036W)|FD-Score:-3.75|P-value:8.95E-5||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPH2(YKL119C)|FD-Score:-6.62|P-value:1.84E-11||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:VPS3(YDR495C)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS45(YGL095C)|FD-Score:-7.51|P-value:2.99E-14||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YAP5(YIR018W)|FD-Score:-3.1|P-value:9.72E-4||SGD DESC:Basic leucine zipper (bZIP) iron-sensing transcription factor; YAP5 has a paralog, YAP7, that arose from the whole genome duplication Gene:YBR196C-A(YBR196C-A_p)|FD-Score:-13|P-value:5.01E-39||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR242W(YBR242W_p)|FD-Score:4.19|P-value:1.38E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YDL034W(YDL034W_d)|FD-Score:4.48|P-value:3.79E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene Gene:YDR290W(YDR290W_d)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YER038W-A(YER038W-A_d)|FD-Score:4.53|P-value:2.98E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria Gene:YHR127W(YHR127W)|FD-Score:-3.85|P-value:5.90E-5||SGD DESC:Protein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis Gene:YKL147C(YKL147C_d)|FD-Score:4.36|P-value:6.43E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene AVT3 Gene:YKL162C(YKL162C_p)|FD-Score:3.73|P-value:9.46E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:YLR225C(YLR225C_p)|FD-Score:4.31|P-value:7.99E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene Gene:YLR312C(YLR312C_p)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Putative protein of unknown function Gene:YLR444C(YLR444C_d)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR245W(YMR245W_d)|FD-Score:-3.78|P-value:7.75E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR005C(YNR005C_d)|FD-Score:-3.98|P-value:3.45E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR071C(YNR071C_p)|FD-Score:4.17|P-value:1.53E-5||SGD DESC:Putative aldose 1-epimerase Gene:YOR318C(YOR318C_d)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo Gene:YOR343C(YOR343C_d)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL080C(YPL080C_d)|FD-Score:-5.97|P-value:1.20E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YSW1(YBR148W)|FD-Score:3.49|P-value:2.37E-4||SGD DESC:Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL112C6.445.93E-111.24TAF6Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4
YBL020W5.201.01E-70.47RFT1Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein
YOR157C4.731.14E-60.09PUP1Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z
YJL011C4.641.78E-60.01RPC17RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress
YLL037W_d4.631.84E-60.06YLL037W_dDubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor
YFR037C4.572.47E-60.10RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YKR071C4.473.97E-60.25DRE2Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress
YAL001C4.221.25E-50.18TFC3Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding
YBR243C4.042.71E-50.03ALG7UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
YGR140W4.013.04E-50.40CBF2Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress
YAL041W3.611.50E-40.07CDC24Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress
YDR016C3.551.94E-40.07DAD1Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YJL081C3.482.48E-40.00ARP4Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
YIL026C3.482.50E-40.03IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YDL092W3.452.78E-40.11SRP14Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBL104C11.903.50E-33SEA4Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites
YJL095W5.891.94E-9BCK1Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
YPL259C5.191.04E-7APM1Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting
YGR108W5.141.38E-7CLB1B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome
YJR117W5.032.40E-7STE24Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans
YOR322C4.592.21E-6LDB19Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye
YER038W-A_d4.532.98E-6YER038W-A_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria
YBR073W4.483.67E-6RDH54DNA-dependent ATPase; stimulates strand exchange by modifying the topology of double-stranded DNA; involved in recombinational repair of DNA double-strand breaks during mitosis and meiosis; proposed to be involved in crossover interference; interacts with Dmc1p; stimulates Dmc1p and Rad51p
YDL034W_d4.483.79E-6YDL034W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene
YJR048W4.405.32E-6CYC1Cytochrome c, isoform 1; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; CYC1 has a paralog, CYC7, that arose from the whole genome duplication
YKL147C_d4.366.43E-6YKL147C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene AVT3
YGL127C4.366.44E-6SOH1Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits
YNL325C4.366.55E-6FIG4Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain
YLR225C_p4.317.99E-6YLR225C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene
YBR242W_p4.191.38E-5YBR242W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene

GO enrichment analysis for SGTC_3293
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1001.07E-14SGTC_8251988-2224 89.6 μMChemDiv (Drug-like library)11808590.0853659cell wall signaling
0.0921.83E-12SGTC_22607964312 200.0 μMChembridge (Fragment library)2319510.125
0.0841.29E-10SGTC_10503232-1156 69.3 μMChemDiv (Drug-like library)7005290.119403RSC complex & mRNA processing
0.0831.66E-10SGTC_2620irigenin 100.0 μMMicrosource (Natural product library)54641700.108108
0.0822.66E-10SGTC_31009120536 49.5 μMChembridge (Drug-like library)164211960.535714
0.0815.32E-10SGTC_486niguldipine 82.0 μMMiscellaneous12360.08amide catabolism
0.0781.95E-9SGTC_22727805186 180.0 μMChembridge (Fragment library)29560620.0540541cell wall signaling
0.0773.51E-9SGTC_32859112818 20.9 μMChembridge (Drug-like library)170179470.1375cell wall
0.0759.32E-9SGTC_1874staurosporine 7.6 μMICCB bioactive library468641930.0947368cell wall signaling
0.0741.20E-8SGTC_22297354935 190.0 μMChembridge (Fragment library)3181120.123077RSC complex & mRNA processing
0.0731.98E-8SGTC_237nikkomycin 200.0 μMMiscellaneous3534810.0576923RSC complex & mRNA processing
0.0715.15E-8SGTC_24215690543 90.7 μMChembridge (Fragment library)33979930.0519481
0.0699.23E-8SGTC_28549024559 71.4 μMChembridge (Drug-like library)31635290.107143
0.0672.40E-7SGTC_29127964298 10.9 μMChembridge (Drug-like library)29758500.126761cell wall
0.0641.01E-6SGTC_650798-0905 35.0 μMChemDiv (Drug-like library)36021450.109756calcium & mitochondrial duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3099912036749.47 μM0.687524824940Chembridge (Drug-like library)405.216293.23405
SGTC_3107912180849.47 μM0.641509662681Chembridge (Drug-like library)309.345742.6406
SGTC_3132912670649.47 μM0.611111850858Chembridge (Drug-like library)326.3481433.56706
SGTC_3123912616249.47 μM0.57894725239884Chembridge (Drug-like library)367.424883.32107
SGTC_3124912756749.47 μM0.55357117592030Chembridge (Drug-like library)323.372322.67406DNA damage response
SGTC_3100912053649.47 μM0.53571416421196Chembridge (Drug-like library)319.340563.25106
SGTC_3122912630949.47 μM0.45762717591206Chembridge (Drug-like library)343.405023.59905cell wall signaling
SGTC_3102912166949.47 μM0.3793141905072Chembridge (Drug-like library)272.368643.57504
SGTC_3292912082559.78 μM0.37288124824900Chembridge (Drug-like library)276.3325233.29405
SGTC_3307912688569.28 μM0.37288124824909Chembridge (Drug-like library)272.368643.77904