9122258

2-[5-[(4-chloro-2-methylphenoxy)methyl]-1H-1,2,4-triazol-3-yl]pyridine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3296
Screen concentration 64.0 μM
Source Chembridge (Drug-like library)
PubChem CID 4730474
SMILES CC1=C(C=CC(=C1)Cl)OCC2=NC(=NN2)C3=CC=CC=N3
Standardized SMILES Cc1cc(Cl)ccc1OCc2n[nH]c(n2)c3ccccn3
Molecular weight 300.7429
ALogP 3.4
H-bond donor count 1
H-bond acceptor count 4
Response signature redox potentiating

Pool Growth Kinetics
% growth inhibition (Het. pool) 15.68
% growth inhibition (Hom. pool) 15.65


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4730474
Download HIP data (tab-delimited text)  (excel)
Gene:AME1(YBR211C)|FD-Score:3.87|P-value:5.47E-5|Clearance:0.01||SGD DESC:Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress Gene:CRM1(YGR218W)|FD-Score:3.56|P-value:1.88E-4|Clearance:0.02||SGD DESC:Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin Gene:DIP2(YLR129W)|FD-Score:-3.73|P-value:9.52E-5|Clearance:0||SGD DESC:Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex Gene:DML1(YMR211W)|FD-Score:4.25|P-value:1.07E-5|Clearance:0.38||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:DSN1(YIR010W)|FD-Score:3.49|P-value:2.38E-4|Clearance:0.05||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation Gene:HTB1(YDR224C)|FD-Score:3.25|P-value:5.84E-4|Clearance:0.07||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:LSG1(YGL099W)|FD-Score:6.41|P-value:7.36E-11|Clearance:0.96||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MCD1(YDL003W)|FD-Score:3.8|P-value:7.37E-5|Clearance:0.07||SGD DESC:Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase Gene:MEX67(YPL169C)|FD-Score:-3.78|P-value:7.92E-5|Clearance:0||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:NIP1(YMR309C)|FD-Score:-3.49|P-value:2.41E-4|Clearance:0||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NOP4(YPL043W)|FD-Score:-3.34|P-value:4.17E-4|Clearance:0||SGD DESC:Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) Gene:PRE1(YER012W)|FD-Score:-3.1|P-value:9.66E-4|Clearance:0||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:RPC82(YPR190C)|FD-Score:-3.41|P-value:3.30E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C82 Gene:RPL18A(YOL120C)|FD-Score:-3.76|P-value:8.43E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPS2(YGL123W)|FD-Score:-3.45|P-value:2.80E-4|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RPS3(YNL178W)|FD-Score:-4.67|P-value:1.52E-6|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP42(YDL111C)|FD-Score:-3.54|P-value:1.98E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:RSC6(YCR052W)|FD-Score:3.44|P-value:2.87E-4|Clearance:0.2||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC31(YDL195W)|FD-Score:4.49|P-value:3.51E-6|Clearance:0.24||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SHQ1(YIL104C)|FD-Score:3.53|P-value:2.06E-4|Clearance:0.04||SGD DESC:Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones Gene:SHR3(YDL212W)|FD-Score:3.72|P-value:9.91E-5|Clearance:0.16||SGD DESC:Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface Gene:SSL2(YIL143C)|FD-Score:3.18|P-value:7.39E-4|Clearance:0.13||SGD DESC:Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; homolog of human ERCC3 Gene:SSU72(YNL222W)|FD-Score:-3.32|P-value:4.51E-4|Clearance:0||SGD DESC:Phosphatase and transcription/RNA-processing factor; associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 and serine-7 of the RNA polymerase II C-terminal domain; affects start site selection and transcriptional read through in vivo Gene:TAF11(YML015C)|FD-Score:5.45|P-value:2.56E-8|Clearance:0.95||SGD DESC:TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors Gene:TEL2(YGR099W)|FD-Score:3.85|P-value:5.80E-5|Clearance:0.06||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:TIF35(YDR429C)|FD-Score:-5.02|P-value:2.52E-7|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TPI1(YDR050C)|FD-Score:-3.71|P-value:1.03E-4|Clearance:0||SGD DESC:Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human TPI causes a rare autosomal disease Gene:UTP6(YDR449C)|FD-Score:-4.32|P-value:7.97E-6|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDR526C(YDR526C_d)|FD-Score:-3.2|P-value:6.85E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR198C(YLR198C_d)|FD-Score:-3.52|P-value:2.19E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Gene:YOR146W(YOR146W_d)|FD-Score:-3.26|P-value:5.55E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:YPR136C(YPR136C_d)|FD-Score:-4.68|P-value:1.42E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 Gene:AME1(YBR211C)|FD-Score:3.87|P-value:5.47E-5|Clearance:0.01||SGD DESC:Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress Gene:CRM1(YGR218W)|FD-Score:3.56|P-value:1.88E-4|Clearance:0.02||SGD DESC:Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin Gene:DIP2(YLR129W)|FD-Score:-3.73|P-value:9.52E-5|Clearance:0||SGD DESC:Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex Gene:DML1(YMR211W)|FD-Score:4.25|P-value:1.07E-5|Clearance:0.38||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:DSN1(YIR010W)|FD-Score:3.49|P-value:2.38E-4|Clearance:0.05||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation Gene:HTB1(YDR224C)|FD-Score:3.25|P-value:5.84E-4|Clearance:0.07||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:LSG1(YGL099W)|FD-Score:6.41|P-value:7.36E-11|Clearance:0.96||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MCD1(YDL003W)|FD-Score:3.8|P-value:7.37E-5|Clearance:0.07||SGD DESC:Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase Gene:MEX67(YPL169C)|FD-Score:-3.78|P-value:7.92E-5|Clearance:0||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:NIP1(YMR309C)|FD-Score:-3.49|P-value:2.41E-4|Clearance:0||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NOP4(YPL043W)|FD-Score:-3.34|P-value:4.17E-4|Clearance:0||SGD DESC:Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) Gene:PRE1(YER012W)|FD-Score:-3.1|P-value:9.66E-4|Clearance:0||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:RPC82(YPR190C)|FD-Score:-3.41|P-value:3.30E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C82 Gene:RPL18A(YOL120C)|FD-Score:-3.76|P-value:8.43E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPS2(YGL123W)|FD-Score:-3.45|P-value:2.80E-4|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RPS3(YNL178W)|FD-Score:-4.67|P-value:1.52E-6|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP42(YDL111C)|FD-Score:-3.54|P-value:1.98E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:RSC6(YCR052W)|FD-Score:3.44|P-value:2.87E-4|Clearance:0.2||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC31(YDL195W)|FD-Score:4.49|P-value:3.51E-6|Clearance:0.24||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SHQ1(YIL104C)|FD-Score:3.53|P-value:2.06E-4|Clearance:0.04||SGD DESC:Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones Gene:SHR3(YDL212W)|FD-Score:3.72|P-value:9.91E-5|Clearance:0.16||SGD DESC:Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface Gene:SSL2(YIL143C)|FD-Score:3.18|P-value:7.39E-4|Clearance:0.13||SGD DESC:Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; homolog of human ERCC3 Gene:SSU72(YNL222W)|FD-Score:-3.32|P-value:4.51E-4|Clearance:0||SGD DESC:Phosphatase and transcription/RNA-processing factor; associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 and serine-7 of the RNA polymerase II C-terminal domain; affects start site selection and transcriptional read through in vivo Gene:TAF11(YML015C)|FD-Score:5.45|P-value:2.56E-8|Clearance:0.95||SGD DESC:TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors Gene:TEL2(YGR099W)|FD-Score:3.85|P-value:5.80E-5|Clearance:0.06||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:TIF35(YDR429C)|FD-Score:-5.02|P-value:2.52E-7|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TPI1(YDR050C)|FD-Score:-3.71|P-value:1.03E-4|Clearance:0||SGD DESC:Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human TPI causes a rare autosomal disease Gene:UTP6(YDR449C)|FD-Score:-4.32|P-value:7.97E-6|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDR526C(YDR526C_d)|FD-Score:-3.2|P-value:6.85E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR198C(YLR198C_d)|FD-Score:-3.52|P-value:2.19E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Gene:YOR146W(YOR146W_d)|FD-Score:-3.26|P-value:5.55E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:YPR136C(YPR136C_d)|FD-Score:-4.68|P-value:1.42E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4730474
Download HOP data (tab-delimited text)  (excel)
Gene:ADA2(YDR448W)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:AFT1(YGL071W)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARL3(YPL051W)|FD-Score:-3.25|P-value:5.84E-4||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:BEM4(YPL161C)|FD-Score:4.47|P-value:3.86E-6||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:CCR4(YAL021C)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CCW12(YLR110C)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:COG8(YML071C)|FD-Score:4.38|P-value:5.83E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CUP2(YGL166W)|FD-Score:7.24|P-value:2.30E-13||SGD DESC:Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication Gene:DEG1(YFL001W)|FD-Score:-3.48|P-value:2.47E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:DST1(YGL043W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:ECM5(YMR176W)|FD-Score:-3.13|P-value:8.86E-4||SGD DESC:Non-essential protein of unknown function; contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress Gene:ERV29(YGR284C)|FD-Score:-4.03|P-value:2.82E-5||SGD DESC:Protein localized to COPII-coated vesicles; involved in vesicle formation and incorporation of specific secretory cargo; protein abundance increases in response to DNA replication stress Gene:FET4(YMR319C)|FD-Score:4.46|P-value:4.05E-6||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GDB1(YPR184W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GRX6(YDL010W)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:GTT3(YEL017W)|FD-Score:-4.89|P-value:5.00E-7||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:GYP1(YOR070C)|FD-Score:-3.25|P-value:5.72E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HIT1(YJR055W)|FD-Score:-3.88|P-value:5.31E-5||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HUR1(YGL168W)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:HXK2(YGL253W)|FD-Score:4.28|P-value:9.25E-6||SGD DESC:Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication Gene:IES2(YNL215W)|FD-Score:3.11|P-value:9.43E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:IMG2(YCR071C)|FD-Score:-5.78|P-value:3.73E-9||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:IRC11(YOR013W_d)|FD-Score:-3.46|P-value:2.69E-4||SGD DESC:Dubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRC14(YOR135C_d)|FD-Score:-3.27|P-value:5.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:ISA1(YLL027W)|FD-Score:-3.27|P-value:5.36E-4||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:ISW1(YBR245C)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions Gene:KTI12(YKL110C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p Gene:MBP1(YDL056W)|FD-Score:-3.98|P-value:3.48E-5||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MNN11(YJL183W)|FD-Score:-3.93|P-value:4.30E-5||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MON1(YGL124C)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MRPL22(YNL177C)|FD-Score:-3.47|P-value:2.58E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL24(YMR193W)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene Gene:MRPL25(YGR076C)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan Gene:MRPL32(YCR003W)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPS28(YDR337W)|FD-Score:-5.71|P-value:5.81E-9||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:PET112(YBL080C)|FD-Score:4.85|P-value:6.12E-7||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PHM6(YDR281C)|FD-Score:5.45|P-value:2.52E-8||SGD DESC:Protein of unknown function, expression is regulated by phosphate levels Gene:PUT1(YLR142W)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Proline oxidase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source Gene:RAM1(YDL090C)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:REC104(YHR157W)|FD-Score:5.58|P-value:1.19E-8||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:REG1(YDR028C)|FD-Score:6.54|P-value:3.05E-11||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RIM13(YMR154C)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM21(YNL294C)|FD-Score:6.36|P-value:9.88E-11||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RIM8(YGL045W)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RIM9(YMR063W)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RNR4(YGR180C)|FD-Score:5.43|P-value:2.76E-8||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RPL26A(YLR344W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication Gene:RRD2(YPL152W)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress Gene:RRT1(YBL048W_d)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:RTF1(YGL244W)|FD-Score:-3.8|P-value:7.11E-5||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SKY1(YMR216C)|FD-Score:-3.17|P-value:7.60E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SLG1(YOR008C)|FD-Score:7.32|P-value:1.28E-13||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SNF8(YPL002C)|FD-Score:3.14|P-value:8.35E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SOL1(YNR034W)|FD-Score:-3.68|P-value:1.16E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SSM4(YIL030C)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation Gene:STF2(YGR008C)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Protein involved in resistance to dessication stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to the F0 sector of mitochondrial F1F0 ATPase in vitro and is proposed to modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress Gene:STO1(YMR125W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:STP22(YCL008C)|FD-Score:7.81|P-value:2.82E-15||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SVP26(YHR181W)|FD-Score:-3.48|P-value:2.55E-4||SGD DESC:Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment Gene:TRP2(YER090W)|FD-Score:-3.91|P-value:4.61E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:-3.88|P-value:5.14E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:-3.89|P-value:5.11E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSR3(YOR006C)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:UME1(YPL139C)|FD-Score:-4.05|P-value:2.57E-5||SGD DESC:Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p Gene:VPH2(YKL119C)|FD-Score:4.01|P-value:3.10E-5||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:VPS20(YMR077C)|FD-Score:4.51|P-value:3.29E-6||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS25(YJR102C)|FD-Score:8.19|P-value:1.27E-16||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS36(YLR417W)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:YBL083C(YBL083C_d)|FD-Score:3.14|P-value:8.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YBR284W(YBR284W_p)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YCR064C(YCR064C_d)|FD-Score:-3.31|P-value:4.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDL062W(YDL062W_d)|FD-Score:-4.17|P-value:1.54E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YDL063C; YDL062W is not essential Gene:YER175W-A(YER175W-A_p)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YER186C(YER186C_p)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Putative protein of unknown function Gene:YGL034C(YGL034C_d)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR122W(YGR122W)|FD-Score:4.53|P-value:2.93E-6||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YGR125W(YGR125W_p)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YGR290W(YGR290W_d)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YJR124C(YJR124C_p)|FD-Score:-3.16|P-value:7.87E-4||SGD DESC:Putative protein of unknown function; expression induced under calcium shortage Gene:YLR031W(YLR031W_p)|FD-Score:5.14|P-value:1.38E-7||SGD DESC:Putative protein of unknown function Gene:YLR111W(YLR111W_d)|FD-Score:7|P-value:1.32E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR184W(YLR184W_d)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR416C(YLR416C_d)|FD-Score:-3.33|P-value:4.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR072W(YOR072W_d)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPL068C(YPL068C_p)|FD-Score:-3.76|P-value:8.55E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YPL080C(YPL080C_d)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR123C(YPR123C_d)|FD-Score:-3.46|P-value:2.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:ZAP1(YJL056C)|FD-Score:5.64|P-value:8.51E-9||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:ZRT3(YKL175W)|FD-Score:4|P-value:3.22E-5||SGD DESC:Vacuolar membrane zinc transporter, transports zinc from storage in the vacuole to the cytoplasm when needed; transcription is induced under conditions of zinc deficiency Gene:ADA2(YDR448W)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:AFT1(YGL071W)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARL3(YPL051W)|FD-Score:-3.25|P-value:5.84E-4||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:BEM4(YPL161C)|FD-Score:4.47|P-value:3.86E-6||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:CCR4(YAL021C)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CCW12(YLR110C)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:COG8(YML071C)|FD-Score:4.38|P-value:5.83E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CUP2(YGL166W)|FD-Score:7.24|P-value:2.30E-13||SGD DESC:Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication Gene:DEG1(YFL001W)|FD-Score:-3.48|P-value:2.47E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:DST1(YGL043W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:ECM5(YMR176W)|FD-Score:-3.13|P-value:8.86E-4||SGD DESC:Non-essential protein of unknown function; contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress Gene:ERV29(YGR284C)|FD-Score:-4.03|P-value:2.82E-5||SGD DESC:Protein localized to COPII-coated vesicles; involved in vesicle formation and incorporation of specific secretory cargo; protein abundance increases in response to DNA replication stress Gene:FET4(YMR319C)|FD-Score:4.46|P-value:4.05E-6||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GDB1(YPR184W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GRX6(YDL010W)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:GTT3(YEL017W)|FD-Score:-4.89|P-value:5.00E-7||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:GYP1(YOR070C)|FD-Score:-3.25|P-value:5.72E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HIT1(YJR055W)|FD-Score:-3.88|P-value:5.31E-5||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HUR1(YGL168W)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:HXK2(YGL253W)|FD-Score:4.28|P-value:9.25E-6||SGD DESC:Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication Gene:IES2(YNL215W)|FD-Score:3.11|P-value:9.43E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:IMG2(YCR071C)|FD-Score:-5.78|P-value:3.73E-9||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:IRC11(YOR013W_d)|FD-Score:-3.46|P-value:2.69E-4||SGD DESC:Dubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRC14(YOR135C_d)|FD-Score:-3.27|P-value:5.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:ISA1(YLL027W)|FD-Score:-3.27|P-value:5.36E-4||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:ISW1(YBR245C)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions Gene:KTI12(YKL110C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p Gene:MBP1(YDL056W)|FD-Score:-3.98|P-value:3.48E-5||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MNN11(YJL183W)|FD-Score:-3.93|P-value:4.30E-5||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MON1(YGL124C)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MRPL22(YNL177C)|FD-Score:-3.47|P-value:2.58E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL24(YMR193W)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene Gene:MRPL25(YGR076C)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan Gene:MRPL32(YCR003W)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPS28(YDR337W)|FD-Score:-5.71|P-value:5.81E-9||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:PET112(YBL080C)|FD-Score:4.85|P-value:6.12E-7||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PHM6(YDR281C)|FD-Score:5.45|P-value:2.52E-8||SGD DESC:Protein of unknown function, expression is regulated by phosphate levels Gene:PUT1(YLR142W)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Proline oxidase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source Gene:RAM1(YDL090C)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:REC104(YHR157W)|FD-Score:5.58|P-value:1.19E-8||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:REG1(YDR028C)|FD-Score:6.54|P-value:3.05E-11||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RIM13(YMR154C)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM21(YNL294C)|FD-Score:6.36|P-value:9.88E-11||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RIM8(YGL045W)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RIM9(YMR063W)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RNR4(YGR180C)|FD-Score:5.43|P-value:2.76E-8||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RPL26A(YLR344W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication Gene:RRD2(YPL152W)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress Gene:RRT1(YBL048W_d)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:RTF1(YGL244W)|FD-Score:-3.8|P-value:7.11E-5||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SKY1(YMR216C)|FD-Score:-3.17|P-value:7.60E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SLG1(YOR008C)|FD-Score:7.32|P-value:1.28E-13||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SNF8(YPL002C)|FD-Score:3.14|P-value:8.35E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SOL1(YNR034W)|FD-Score:-3.68|P-value:1.16E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SSM4(YIL030C)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation Gene:STF2(YGR008C)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Protein involved in resistance to dessication stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to the F0 sector of mitochondrial F1F0 ATPase in vitro and is proposed to modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress Gene:STO1(YMR125W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:STP22(YCL008C)|FD-Score:7.81|P-value:2.82E-15||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SVP26(YHR181W)|FD-Score:-3.48|P-value:2.55E-4||SGD DESC:Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment Gene:TRP2(YER090W)|FD-Score:-3.91|P-value:4.61E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:-3.88|P-value:5.14E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:-3.89|P-value:5.11E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSR3(YOR006C)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:UME1(YPL139C)|FD-Score:-4.05|P-value:2.57E-5||SGD DESC:Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p Gene:VPH2(YKL119C)|FD-Score:4.01|P-value:3.10E-5||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:VPS20(YMR077C)|FD-Score:4.51|P-value:3.29E-6||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS25(YJR102C)|FD-Score:8.19|P-value:1.27E-16||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS36(YLR417W)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:YBL083C(YBL083C_d)|FD-Score:3.14|P-value:8.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YBR284W(YBR284W_p)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YCR064C(YCR064C_d)|FD-Score:-3.31|P-value:4.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDL062W(YDL062W_d)|FD-Score:-4.17|P-value:1.54E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YDL063C; YDL062W is not essential Gene:YER175W-A(YER175W-A_p)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YER186C(YER186C_p)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Putative protein of unknown function Gene:YGL034C(YGL034C_d)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR122W(YGR122W)|FD-Score:4.53|P-value:2.93E-6||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YGR125W(YGR125W_p)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YGR290W(YGR290W_d)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YJR124C(YJR124C_p)|FD-Score:-3.16|P-value:7.87E-4||SGD DESC:Putative protein of unknown function; expression induced under calcium shortage Gene:YLR031W(YLR031W_p)|FD-Score:5.14|P-value:1.38E-7||SGD DESC:Putative protein of unknown function Gene:YLR111W(YLR111W_d)|FD-Score:7|P-value:1.32E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR184W(YLR184W_d)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR416C(YLR416C_d)|FD-Score:-3.33|P-value:4.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR072W(YOR072W_d)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPL068C(YPL068C_p)|FD-Score:-3.76|P-value:8.55E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YPL080C(YPL080C_d)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR123C(YPR123C_d)|FD-Score:-3.46|P-value:2.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:ZAP1(YJL056C)|FD-Score:5.64|P-value:8.51E-9||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:ZRT3(YKL175W)|FD-Score:4|P-value:3.22E-5||SGD DESC:Vacuolar membrane zinc transporter, transports zinc from storage in the vacuole to the cytoplasm when needed; transcription is induced under conditions of zinc deficiency

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W6.417.36E-110.96LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YML015C5.452.56E-80.95TAF11TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors
YDL195W4.493.51E-60.24SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YMR211W4.251.07E-50.38DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YBR211C3.875.47E-50.01AME1Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress
YGR099W3.855.80E-50.06TEL2Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1
YDL003W3.807.37E-50.07MCD1Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase
YDL212W3.729.91E-50.17SHR3Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface
YGR218W3.561.88E-40.02CRM1Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin
YIL104C3.532.06E-40.04SHQ1Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones
YIR010W3.492.38E-40.05DSN1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation
YCR052W3.442.87E-40.20RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YDR224C3.255.84E-40.07HTB1Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation
YIL143C3.187.39E-40.13SSL2Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; homolog of human ERCC3
YHR170W3.050.001150.13NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJR102C8.191.27E-16VPS25Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome
YCL008C7.812.82E-15STP22Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype
YOR008C7.321.28E-13SLG1Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response
YGL166W7.242.30E-13CUP2Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication
YLR111W_d7.001.32E-12YLR111W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDR028C6.543.05E-11REG1Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p
YNL294C6.369.88E-11RIM21pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH
YJL056C5.648.51E-9ZAP1Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains
YHR157W5.581.19E-8REC104Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination
YDR281C5.452.52E-8PHM6Protein of unknown function, expression is regulated by phosphate levels
YGR180C5.432.76E-8RNR4Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress
YLR031W_p5.141.38E-7YLR031W_pPutative protein of unknown function
YBL080C4.856.12E-7PET112Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog
YGR122W4.532.93E-6YGR122WProtein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress
YMR077C4.513.29E-6VPS20Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes

GO enrichment analysis for SGTC_3296
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2432.24E-80SGTC_18845633444 25.0 μMMiscellaneous57242390.231884redox potentiating
0.2179.24E-64SGTC_525k781-2675 26.7 μMChemDiv (Drug-like library)36511180.161765redox potentiating
0.1741.42E-41SGTC_10441222-0021 1.8 μMChemDiv (Drug-like library)43067030.0909091heme biosynthesis & mitochondrial translocase
0.1721.21E-40SGTC_5761073-0113 22.7 μMChemDiv (Drug-like library)54395630.139535redox potentiating
0.1694.96E-39SGTC_102gallobenzophenone 83.6 μMChemDiv (Drug-like library)708370.0769231redox potentiating
0.1533.06E-32SGTC_31109122132 49.5 μMChembridge (Drug-like library)9183170.6redox potentiating
0.1391.10E-26SGTC_24855633444 17.8 μMMiscellaneous57242390.231884redox potentiating
0.1374.61E-26SGTC_1788fluconazole 20.0 μMMiscellaneous33650.0810811azole & statin
0.1375.76E-26SGTC_13171273-0028 910.0 nMChemDiv (Drug-like library)59178030.0714286azole & statin
0.1344.07E-25SGTC_1593justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one 54.9 μMTimTec (Pure natural product library)4428820.0853659redox potentiating
0.1314.61E-24SGTC_15924',5'-dihydroxyflavone 8.8 μMTimTec (Pure natural product library)6886690.094594660S ribosome export
0.1255.45E-22SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.11688360S ribosome export
0.1241.30E-21SGTC_1829berberine 14.4 μMTimTec (Natural product derivative library)23530.096385560S ribosome export
0.1232.68E-21SGTC_13111190-0148 100.0 μMChemDiv (Drug-like library)19613330.0769231azole & statin
0.1202.97E-20SGTC_1827berberine 7.2 μMTimTec (Natural product derivative library)6294130.096385560S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3295912236253.57 μM0.6140354730473Chembridge (Drug-like library)349.214564.14614
SGTC_3110912213249.47 μM0.6918317Chembridge (Drug-like library)252.271282.24814redox potentiating
SGTC_3214534-389065.3 μM0.409091699746ChemDiv (Drug-like library)345.82333.24704RPP1 & pyrimidine depletion
SGTC_15120259-054412.2 μM0.34426218476ChemDiv (Drug-like library)293.1484.43412
SGTC_2836901033819.48 μM0.32994051Chembridge (Drug-like library)315.754263.89305RPP1 & pyrimidine depletion
SGTC_12001643-019318.4 μM0.2763165403974ChemDiv (Drug-like library)347.753063.56315
SGTC_3123912616249.47 μM0.27397325239884Chembridge (Drug-like library)367.424883.32107
SGTC_1974544-004341.56 μM0.264706896523ChemDiv (Drug-like library)322.189224.30713endomembrane recycling
SGTC_21065357045167.29 μM0.261538668842Chembridge (Fragment library)281.13733.01412
SGTC_14514152-0210104 μM0.26659563ChemDiv (Drug-like library)234.2562.66104