9124615

6-(4-methoxyphenyl)-3-(2-methylpropyl)-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3300
Screen concentration 50.5 μM
Source Chembridge (Drug-like library)
PubChem CID 24824903
SMILES CC(C)CC1=NN=C2N1N=C(S2)C3=CC=C(C=C3)OC
Standardized SMILES COc1ccc(cc1)c2nn3c(CC(C)C)nnc3s2
Molecular weight 288.368
ALogP 3.07
H-bond donor count 0
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.89
% growth inhibition (Hom. pool) 11.4


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 24824903
Download HIP data (tab-delimited text)  (excel)
Gene:ADE13(YLR359W)|FD-Score:3.45|P-value:2.85E-4|Clearance:0.06||SGD DESC:Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency Gene:ARC35(YNR035C)|FD-Score:3.38|P-value:3.58E-4|Clearance:0.02||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:DBP5(YOR046C)|FD-Score:-6.56|P-value:2.73E-11|Clearance:0||SGD DESC:Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p Gene:MED8(YBR193C)|FD-Score:4.19|P-value:1.40E-5|Clearance:0.74||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NMT1(YLR195C)|FD-Score:3.25|P-value:5.78E-4|Clearance:0.15||SGD DESC:N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction Gene:NUP49(YGL172W)|FD-Score:3.1|P-value:9.53E-4|Clearance:0.04||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:PRI1(YIR008C)|FD-Score:3.35|P-value:4.09E-4|Clearance:0.1||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RPC17(YJL011C)|FD-Score:-4.56|P-value:2.53E-6|Clearance:0||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPF1(YHR088W)|FD-Score:-3.51|P-value:2.25E-4|Clearance:0||SGD DESC:Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA Gene:RRP36(YOR287C)|FD-Score:-4.11|P-value:1.99E-5|Clearance:0||SGD DESC:Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit Gene:TEL2(YGR099W)|FD-Score:3.36|P-value:3.88E-4|Clearance:0.01||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:TFC3(YAL001C)|FD-Score:-3.84|P-value:6.26E-5|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TOA2(YKL058W)|FD-Score:-3.58|P-value:1.73E-4|Clearance:0||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:YDL163W(YDL163W_d)|FD-Score:-3.28|P-value:5.14E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase Gene:ADE13(YLR359W)|FD-Score:3.45|P-value:2.85E-4|Clearance:0.06||SGD DESC:Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency Gene:ARC35(YNR035C)|FD-Score:3.38|P-value:3.58E-4|Clearance:0.02||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:DBP5(YOR046C)|FD-Score:-6.56|P-value:2.73E-11|Clearance:0||SGD DESC:Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p Gene:MED8(YBR193C)|FD-Score:4.19|P-value:1.40E-5|Clearance:0.74||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NMT1(YLR195C)|FD-Score:3.25|P-value:5.78E-4|Clearance:0.15||SGD DESC:N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction Gene:NUP49(YGL172W)|FD-Score:3.1|P-value:9.53E-4|Clearance:0.04||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:PRI1(YIR008C)|FD-Score:3.35|P-value:4.09E-4|Clearance:0.1||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RPC17(YJL011C)|FD-Score:-4.56|P-value:2.53E-6|Clearance:0||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPF1(YHR088W)|FD-Score:-3.51|P-value:2.25E-4|Clearance:0||SGD DESC:Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA Gene:RRP36(YOR287C)|FD-Score:-4.11|P-value:1.99E-5|Clearance:0||SGD DESC:Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit Gene:TEL2(YGR099W)|FD-Score:3.36|P-value:3.88E-4|Clearance:0.01||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:TFC3(YAL001C)|FD-Score:-3.84|P-value:6.26E-5|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TOA2(YKL058W)|FD-Score:-3.58|P-value:1.73E-4|Clearance:0||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:YDL163W(YDL163W_d)|FD-Score:-3.28|P-value:5.14E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 24824903
Download HOP data (tab-delimited text)  (excel)
Gene:AIM11(YER093C-A)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication Gene:ALR2(YFL050C)|FD-Score:-3.22|P-value:6.51E-4||SGD DESC:Probable Mg(2+) transporter; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions; plays a role in regulating Ty1 transposition Gene:AQY1(YPR192W)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Spore-specific water channel that mediates the transport of water across cell membranes, developmentally controlled; may play a role in spore maturation, probably by allowing water outflow, may be involved in freeze tolerance Gene:ATG29(YPL166W)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:AUS1(YOR011W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen Gene:BAP2(YBR068C)|FD-Score:-3.18|P-value:7.35E-4||SGD DESC:High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication Gene:BUD25(YER014C-A)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern Gene:CAM1(YPL048W)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma Gene:CBR1(YIL043C)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CSH1(YBR161W)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:ECM8(YBR076W)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Non-essential protein of unknown function Gene:ELA1(YNL230C)|FD-Score:5.59|P-value:1.14E-8||SGD DESC:Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex Gene:FAA4(YMR246W)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:FAT1(YBR041W)|FD-Score:4.57|P-value:2.43E-6||SGD DESC:Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids Gene:FLO8(YER109C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene Gene:FMP16(YDR070C_p)|FD-Score:-3.12|P-value:9.04E-4||SGD DESC:Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:GCN20(YFR009W)|FD-Score:-5.96|P-value:1.23E-9||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GPA2(YER020W)|FD-Score:-3.94|P-value:4.02E-5||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:GTB1(YDR221W)|FD-Score:5.18|P-value:1.11E-7||SGD DESC:Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress Gene:GYP7(YDL234C)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:GTPase-activating protein for yeast Rab family members including: Ypt7p (most effective), Ypt1p, Ypt31p, and Ypt32p (in vitro); involved in vesicle mediated protein trafficking Gene:HHT1(YBR010W)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation Gene:HIS2(YFR025C)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Histidinolphosphatase, catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:IDH2(YOR136W)|FD-Score:-3.33|P-value:4.39E-4||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IMO32(YGR031W)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1 Gene:KAP114(YGL241W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress Gene:KAR4(YCL055W)|FD-Score:-3.25|P-value:5.80E-4||SGD DESC:Transcription factor required for gene regulation in response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone Gene:MCR1(YKL150W)|FD-Score:-5.23|P-value:8.33E-8||SGD DESC:Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis Gene:MGM101(YJR144W)|FD-Score:-3.81|P-value:7.03E-5||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MSN4(YKL062W)|FD-Score:4.62|P-value:1.92E-6||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN4 has a paralog, MSN2, that arose from the whole genome duplication Gene:MSO1(YNR049C)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress Gene:MTC7(YEL033W_p)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:NCE101(YJL205C)|FD-Score:-3.94|P-value:4.09E-5||SGD DESC:Protein of unknown function, involved in secretion of proteins that lack classical secretory signal sequences Gene:PAH1(YMR165C)|FD-Score:-3.25|P-value:5.76E-4||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PDR1(YGL013C)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:POR1(YNL055C)|FD-Score:-3.54|P-value:2.03E-4||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:PTK2(YJR059W)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PUT4(YOR348C)|FD-Score:-3.4|P-value:3.41E-4||SGD DESC:Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells Gene:RCF1(YML030W)|FD-Score:-3.77|P-value:8.12E-5||SGD DESC:Cytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast Gene:RGI2(YIL057C)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:Protein of unknown function involved in energy metabolism under respiratory conditions; expression induced under carbon limitation and repressed under high glucose Gene:RNR4(YGR180C)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RNY1(YPL123C)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Vacuolar RNase of the T(2) family, relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; promotes apoptosis under stress conditions and this function is independent of its catalytic activity Gene:RPL26B(YGR034W)|FD-Score:-3.96|P-value:3.71E-5||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPL36A(YMR194W)|FD-Score:3.11|P-value:9.48E-4||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:RRT2(YBR246W)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:RTT107(YHR154W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SET6(YPL165C)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:SET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability Gene:SNO4(YMR322C_p)|FD-Score:4.53|P-value:2.93E-6||SGD DESC:Possible chaperone and cysteine protease, similar to bacterial Hsp31 and yeast Hsp31p, Hsp32p, and Hsp33p; DJ-1/ThiJ/PfpI superfamily member; predicted involvement in pyridoxine metabolism; induced by mild heat stress and copper deprivation Gene:SPR28(YDR218C)|FD-Score:-7.24|P-value:2.32E-13||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; meiotic septin expressed at high levels during meiotic divisions and ascospore formation Gene:STM1(YLR150W)|FD-Score:4.79|P-value:8.23E-7||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:STO1(YMR125W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SUC2(YIL162W)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:TGL4(YKR089C)|FD-Score:5.24|P-value:7.84E-8||SGD DESC:Multifunctional triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p Gene:THR4(YCR053W)|FD-Score:4.1|P-value:2.09E-5||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TYE7(YOR344C)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Serine-rich protein that contains a basic-helix-loop-helix (bHLH) DNA binding motif; binds E-boxes of glycolytic genes and contributes to their activation; may function as a transcriptional activator in Ty1-mediated gene expression Gene:UBC8(YEL012W)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro Gene:UBP2(YOR124C)|FD-Score:4.37|P-value:6.34E-6||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; interacts with Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity Gene:VPS24(YKL041W)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:YCF1(YDR135C)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Vacuolar glutathione S-conjugate transporter; member of the ATP-binding cassette family; has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR Gene:YDL180W(YDL180W_p)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YDL211C(YDL211C_p)|FD-Score:-6.46|P-value:5.31E-11||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YDR248C(YDR248C_p)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:Putative gluconokinase; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1 Gene:YDR431W(YDR431W_d)|FD-Score:3.81|P-value:6.98E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER066C-A(YER066C-A_d)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, partially overlaps uncharacterized ORF YER067W Gene:YGL010W(YGL010W_p)|FD-Score:5.06|P-value:2.07E-7||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YGL041C-B(YGL041C-B_p)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGR066C(YGR066C_p)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Putative protein of unknown function Gene:YGR176W(YGR176W_d)|FD-Score:4.5|P-value:3.43E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR175W-A(YHR175W-A_p)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YIP3(YNL044W)|FD-Score:-3.21|P-value:6.57E-4||SGD DESC:Protein localized to COPII vesicles, proposed to be involved in ER to Golgi transport; interacts with members of the Rab GTPase family and Yip1p; also interacts with Rtn1p Gene:YKL068W-A(YKL068W-A_p)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YLR012C(YLR012C_p)|FD-Score:-3.6|P-value:1.56E-4||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YMR166C(YMR166C_p)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YMR206W(YMR206W_p)|FD-Score:8.09|P-value:3.02E-16||SGD DESC:Putative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication Gene:YNK1(YKL067W)|FD-Score:3.8|P-value:7.16E-5||SGD DESC:Nucleoside diphosphate kinase; catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate; protein abundance increases in response to DNA replication stress Gene:YNL143C(YNL143C_p)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YNR066C(YNR066C_p)|FD-Score:-3.26|P-value:5.51E-4||SGD DESC:Putative membrane-localized protein of unknown function Gene:YOR199W(YOR199W_d)|FD-Score:3.83|P-value:6.32E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR228C(YOR228C)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YPL077C(YPL077C_p)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication Gene:YPL225W(YPL225W)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPR063C(YPR063C_p)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:ER-localized protein of unknown function Gene:YPR092W(YPR092W_d)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR172W(YPR172W_p)|FD-Score:-3.8|P-value:7.32E-5||SGD DESC:Putative pyridoxal 5'-phosphate synthase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; YPR172W has a paralog, YLR456W, that arose from the whole genome duplication Gene:YRB30(YGL164C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes Gene:AIM11(YER093C-A)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication Gene:ALR2(YFL050C)|FD-Score:-3.22|P-value:6.51E-4||SGD DESC:Probable Mg(2+) transporter; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions; plays a role in regulating Ty1 transposition Gene:AQY1(YPR192W)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Spore-specific water channel that mediates the transport of water across cell membranes, developmentally controlled; may play a role in spore maturation, probably by allowing water outflow, may be involved in freeze tolerance Gene:ATG29(YPL166W)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:AUS1(YOR011W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen Gene:BAP2(YBR068C)|FD-Score:-3.18|P-value:7.35E-4||SGD DESC:High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication Gene:BUD25(YER014C-A)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern Gene:CAM1(YPL048W)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma Gene:CBR1(YIL043C)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CSH1(YBR161W)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:ECM8(YBR076W)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Non-essential protein of unknown function Gene:ELA1(YNL230C)|FD-Score:5.59|P-value:1.14E-8||SGD DESC:Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex Gene:FAA4(YMR246W)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:FAT1(YBR041W)|FD-Score:4.57|P-value:2.43E-6||SGD DESC:Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids Gene:FLO8(YER109C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene Gene:FMP16(YDR070C_p)|FD-Score:-3.12|P-value:9.04E-4||SGD DESC:Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:GCN20(YFR009W)|FD-Score:-5.96|P-value:1.23E-9||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GPA2(YER020W)|FD-Score:-3.94|P-value:4.02E-5||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:GTB1(YDR221W)|FD-Score:5.18|P-value:1.11E-7||SGD DESC:Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress Gene:GYP7(YDL234C)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:GTPase-activating protein for yeast Rab family members including: Ypt7p (most effective), Ypt1p, Ypt31p, and Ypt32p (in vitro); involved in vesicle mediated protein trafficking Gene:HHT1(YBR010W)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation Gene:HIS2(YFR025C)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Histidinolphosphatase, catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:IDH2(YOR136W)|FD-Score:-3.33|P-value:4.39E-4||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IMO32(YGR031W)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1 Gene:KAP114(YGL241W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress Gene:KAR4(YCL055W)|FD-Score:-3.25|P-value:5.80E-4||SGD DESC:Transcription factor required for gene regulation in response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone Gene:MCR1(YKL150W)|FD-Score:-5.23|P-value:8.33E-8||SGD DESC:Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis Gene:MGM101(YJR144W)|FD-Score:-3.81|P-value:7.03E-5||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MSN4(YKL062W)|FD-Score:4.62|P-value:1.92E-6||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN4 has a paralog, MSN2, that arose from the whole genome duplication Gene:MSO1(YNR049C)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress Gene:MTC7(YEL033W_p)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:NCE101(YJL205C)|FD-Score:-3.94|P-value:4.09E-5||SGD DESC:Protein of unknown function, involved in secretion of proteins that lack classical secretory signal sequences Gene:PAH1(YMR165C)|FD-Score:-3.25|P-value:5.76E-4||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PDR1(YGL013C)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:POR1(YNL055C)|FD-Score:-3.54|P-value:2.03E-4||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:PTK2(YJR059W)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PUT4(YOR348C)|FD-Score:-3.4|P-value:3.41E-4||SGD DESC:Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells Gene:RCF1(YML030W)|FD-Score:-3.77|P-value:8.12E-5||SGD DESC:Cytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast Gene:RGI2(YIL057C)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:Protein of unknown function involved in energy metabolism under respiratory conditions; expression induced under carbon limitation and repressed under high glucose Gene:RNR4(YGR180C)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RNY1(YPL123C)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Vacuolar RNase of the T(2) family, relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; promotes apoptosis under stress conditions and this function is independent of its catalytic activity Gene:RPL26B(YGR034W)|FD-Score:-3.96|P-value:3.71E-5||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPL36A(YMR194W)|FD-Score:3.11|P-value:9.48E-4||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:RRT2(YBR246W)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:RTT107(YHR154W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SET6(YPL165C)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:SET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability Gene:SNO4(YMR322C_p)|FD-Score:4.53|P-value:2.93E-6||SGD DESC:Possible chaperone and cysteine protease, similar to bacterial Hsp31 and yeast Hsp31p, Hsp32p, and Hsp33p; DJ-1/ThiJ/PfpI superfamily member; predicted involvement in pyridoxine metabolism; induced by mild heat stress and copper deprivation Gene:SPR28(YDR218C)|FD-Score:-7.24|P-value:2.32E-13||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; meiotic septin expressed at high levels during meiotic divisions and ascospore formation Gene:STM1(YLR150W)|FD-Score:4.79|P-value:8.23E-7||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:STO1(YMR125W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SUC2(YIL162W)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:TGL4(YKR089C)|FD-Score:5.24|P-value:7.84E-8||SGD DESC:Multifunctional triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p Gene:THR4(YCR053W)|FD-Score:4.1|P-value:2.09E-5||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TYE7(YOR344C)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Serine-rich protein that contains a basic-helix-loop-helix (bHLH) DNA binding motif; binds E-boxes of glycolytic genes and contributes to their activation; may function as a transcriptional activator in Ty1-mediated gene expression Gene:UBC8(YEL012W)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro Gene:UBP2(YOR124C)|FD-Score:4.37|P-value:6.34E-6||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; interacts with Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity Gene:VPS24(YKL041W)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:YCF1(YDR135C)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Vacuolar glutathione S-conjugate transporter; member of the ATP-binding cassette family; has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR Gene:YDL180W(YDL180W_p)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YDL211C(YDL211C_p)|FD-Score:-6.46|P-value:5.31E-11||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YDR248C(YDR248C_p)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:Putative gluconokinase; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1 Gene:YDR431W(YDR431W_d)|FD-Score:3.81|P-value:6.98E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER066C-A(YER066C-A_d)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, partially overlaps uncharacterized ORF YER067W Gene:YGL010W(YGL010W_p)|FD-Score:5.06|P-value:2.07E-7||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YGL041C-B(YGL041C-B_p)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGR066C(YGR066C_p)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Putative protein of unknown function Gene:YGR176W(YGR176W_d)|FD-Score:4.5|P-value:3.43E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR175W-A(YHR175W-A_p)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YIP3(YNL044W)|FD-Score:-3.21|P-value:6.57E-4||SGD DESC:Protein localized to COPII vesicles, proposed to be involved in ER to Golgi transport; interacts with members of the Rab GTPase family and Yip1p; also interacts with Rtn1p Gene:YKL068W-A(YKL068W-A_p)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YLR012C(YLR012C_p)|FD-Score:-3.6|P-value:1.56E-4||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YMR166C(YMR166C_p)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YMR206W(YMR206W_p)|FD-Score:8.09|P-value:3.02E-16||SGD DESC:Putative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication Gene:YNK1(YKL067W)|FD-Score:3.8|P-value:7.16E-5||SGD DESC:Nucleoside diphosphate kinase; catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate; protein abundance increases in response to DNA replication stress Gene:YNL143C(YNL143C_p)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YNR066C(YNR066C_p)|FD-Score:-3.26|P-value:5.51E-4||SGD DESC:Putative membrane-localized protein of unknown function Gene:YOR199W(YOR199W_d)|FD-Score:3.83|P-value:6.32E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR228C(YOR228C)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YPL077C(YPL077C_p)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication Gene:YPL225W(YPL225W)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPR063C(YPR063C_p)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:ER-localized protein of unknown function Gene:YPR092W(YPR092W_d)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR172W(YPR172W_p)|FD-Score:-3.8|P-value:7.32E-5||SGD DESC:Putative pyridoxal 5'-phosphate synthase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; YPR172W has a paralog, YLR456W, that arose from the whole genome duplication Gene:YRB30(YGL164C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR193C4.191.40E-50.74MED8Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YLR359W3.452.85E-40.06ADE13Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency
YNR035C3.383.58E-40.02ARC35Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin
YGR099W3.363.88E-40.01TEL2Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1
YIR008C3.354.09E-40.10PRI1Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
YLR195C3.255.78E-40.14NMT1N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction
YGL172W3.109.53E-40.04NUP49FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p)
YIL144W3.070.001080.17TID3Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering
YBR254C2.900.001890.05TRS20One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder
YML046W2.840.002240.05PRP39U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats
YDR473C2.790.002630.20PRP3Splicing factor, component of the U4/U6-U5 snRNP complex
YBR256C2.590.004840.01RIB5Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway
YNL260C_p2.580.004930.12LTO1_pEssential protein that forms a complex with Rli1p and Yae1p; ortholog of human ORAOV1, which is overexpressed in solid tumors; inviability of null mutant under standard conditions is complemented by overexpression of ORAOV1; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species
YJL019W2.460.006930.02MPS3Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
YOR168W2.440.007290.01GLN4Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR206W_p8.093.02E-16YMR206W_pPutative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication
YNL230C5.591.14E-8ELA1Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex
YKR089C5.247.84E-8TGL4Multifunctional triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p
YDR221W5.181.11E-7GTB1Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress
YGL010W_p5.062.07E-7YGL010W_pPutative protein of unknown function; YGL010W is not an essential gene
YLR150W4.798.23E-7STM1Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
YGL041C-B_p4.721.19E-6YGL041C-B_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YKL062W4.621.92E-6MSN4Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN4 has a paralog, MSN2, that arose from the whole genome duplication
YMR246W4.621.94E-6FAA4Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication
YBR041W4.572.43E-6FAT1Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids
YMR322C_p4.532.93E-6SNO4_pPossible chaperone and cysteine protease, similar to bacterial Hsp31 and yeast Hsp31p, Hsp32p, and Hsp33p; DJ-1/ThiJ/PfpI superfamily member; predicted involvement in pyridoxine metabolism; induced by mild heat stress and copper deprivation
YGR176W_d4.503.43E-6YGR176W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YOR124C4.376.34E-6UBP2Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; interacts with Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity
YGL013C4.221.20E-5PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YER093C-A4.211.25E-5AIM11Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication

GO enrichment analysis for SGTC_3300
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0681.68E-7SGTC_295k015-0027 31.0 μMChemDiv (Drug-like library)38874240.0684932PDR1
0.0672.74E-7SGTC_1700st030840 7.9 μMTimTec (Natural product derivative library)54993070.0909091PDR1
0.0664.52E-7SGTC_33109130229 44.1 μMChembridge (Drug-like library)77612580.114286Golgi
0.0621.67E-6SGTC_33029125395 16.4 μMChembridge (Drug-like library)175996680.471698
0.0595.60E-6SGTC_12430416-0003 5.7 μMChemDiv (Drug-like library)21419770.0491803PDR1
0.0587.10E-6SGTC_250brefeldin a 552.2 μMICCB bioactive library64361870.038961PDR1
0.0543.31E-5SGTC_10084106-0037 6.0 μMChemDiv (Drug-like library)29053940.133333PDR1
0.0517.90E-5SGTC_5983937-0236 20.2 μMChemDiv (Drug-like library)13793130.0547945ERG2
0.0501.32E-4SGTC_31469095609 49.5 μMChembridge (Drug-like library)171739990.101266
0.0491.78E-4SGTC_32419133421 49.5 μMChembridge (Drug-like library)170182210.0444444PDR1
0.0481.96E-4SGTC_2508celastrol 40.5 μMMicrosource (Natural product library)1227240.0333333PDR1
0.0481.97E-4SGTC_5274476-4215 268.0 μMChemDiv (Drug-like library)44187540.0421053PDR1
0.0482.57E-4SGTC_8520418-0143 439.0 μMChemDiv (Drug-like library)53692290.0151515calcium & mitochondrial duress
0.0472.72E-4SGTC_196k015-0023 18.1 μMChemDiv (Drug-like library)52119980.0540541PDR1
0.0473.36E-4SGTC_1139k015-0024 46.9 μMChemDiv (Drug-like library)27666520.0555556PDR1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3299912198243.13 μM0.64583317594604Chembridge (Drug-like library)273.35672.22105
SGTC_3073911497249.47 μM0.61224541905075Chembridge (Drug-like library)272.368643.5750460S ribosome export
SGTC_3305912702753.83 μM0.59615441905295Chembridge (Drug-like library)316.378142.87506
SGTC_3301912535419.8 μM0.57692341905162Chembridge (Drug-like library)301.409863.25105
SGTC_3102912166949.47 μM0.57142941905072Chembridge (Drug-like library)272.368643.57504
SGTC_3306912767659.79 μM0.57142941905287Chembridge (Drug-like library)264.369783.04205
SGTC_3292912082559.78 μM0.5624824900Chembridge (Drug-like library)276.3325233.29405
SGTC_3116912494349.47 μM0.51923141905195Chembridge (Drug-like library)288.368042.92405
SGTC_3101912053149.47 μM0.50909124824904Chembridge (Drug-like library)288.368043.27705
SGTC_2891906387619.48 μM0.56472478Chembridge (Drug-like library)312.346382.79206