9125354

N,N-dimethyl-3-[3-(2-methylpropyl)-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazol-6-yl]aniline

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3301
Screen concentration 19.8 μM
Source Chembridge (Drug-like library)
PubChem CID 41905162
SMILES CC(C)CC1=NN=C2N1N=C(S2)C3=CC(=CC=C3)N(C)C
Standardized SMILES CC(C)Cc1nnc2sc(nn12)c3cccc(c3)N(C)C
Molecular weight 301.4099
ALogP 3.25
H-bond donor count 0
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 15.68
% growth inhibition (Hom. pool) 11.4


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 41905162
Download HIP data (tab-delimited text)  (excel)
Gene:BMS1(YPL217C)|FD-Score:4|P-value:3.18E-5|Clearance:0.56||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CWC2(YDL209C)|FD-Score:3.13|P-value:8.72E-4|Clearance:0.03||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:DRE2(YKR071C)|FD-Score:3.16|P-value:7.89E-4|Clearance:0.01||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:FAS1(YKL182W)|FD-Score:5.51|P-value:1.76E-8|Clearance:1.51||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:GWT1(YJL091C)|FD-Score:3.3|P-value:4.89E-4|Clearance:0.13||SGD DESC:Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors Gene:RHO3(YIL118W)|FD-Score:3.1|P-value:9.77E-4|Clearance:0.23||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p Gene:RPP1(YHR062C)|FD-Score:-3.49|P-value:2.38E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SRP72(YPL210C)|FD-Score:3.17|P-value:7.59E-4|Clearance:0.01||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:TRM5(YHR070W)|FD-Score:-3.39|P-value:3.49E-4|Clearance:0||SGD DESC:tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya Gene:TUB4(YLR212C)|FD-Score:-3.37|P-value:3.71E-4|Clearance:0||SGD DESC:Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress Gene:UBA1(YKL210W)|FD-Score:-3.3|P-value:4.80E-4|Clearance:0||SGD DESC:Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress Gene:UTP7(YER082C)|FD-Score:3.44|P-value:2.89E-4|Clearance:0.14||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YPR177C(YPR177C_d)|FD-Score:3.15|P-value:8.04E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex Gene:BMS1(YPL217C)|FD-Score:4|P-value:3.18E-5|Clearance:0.56||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CWC2(YDL209C)|FD-Score:3.13|P-value:8.72E-4|Clearance:0.03||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:DRE2(YKR071C)|FD-Score:3.16|P-value:7.89E-4|Clearance:0.01||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:FAS1(YKL182W)|FD-Score:5.51|P-value:1.76E-8|Clearance:1.51||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:GWT1(YJL091C)|FD-Score:3.3|P-value:4.89E-4|Clearance:0.13||SGD DESC:Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors Gene:RHO3(YIL118W)|FD-Score:3.1|P-value:9.77E-4|Clearance:0.23||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p Gene:RPP1(YHR062C)|FD-Score:-3.49|P-value:2.38E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SRP72(YPL210C)|FD-Score:3.17|P-value:7.59E-4|Clearance:0.01||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:TRM5(YHR070W)|FD-Score:-3.39|P-value:3.49E-4|Clearance:0||SGD DESC:tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya Gene:TUB4(YLR212C)|FD-Score:-3.37|P-value:3.71E-4|Clearance:0||SGD DESC:Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress Gene:UBA1(YKL210W)|FD-Score:-3.3|P-value:4.80E-4|Clearance:0||SGD DESC:Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress Gene:UTP7(YER082C)|FD-Score:3.44|P-value:2.89E-4|Clearance:0.14||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YPR177C(YPR177C_d)|FD-Score:3.15|P-value:8.04E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 41905162
Download HOP data (tab-delimited text)  (excel)
Gene:AIM19(YIL087C)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth Gene:ATP19(YOL077W-A)|FD-Score:-3.76|P-value:8.53E-5||SGD DESC:Subunit k of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase Gene:AVT4(YNL101W)|FD-Score:4.37|P-value:6.18E-6||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BTN2(YGR142W)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; BTN2 has a paralog, CUR1, that arose from the whole genome duplication Gene:CKI1(YLR133W)|FD-Score:-3.37|P-value:3.70E-4||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:DCI1(YOR180C)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed Gene:DOT6(YER088C)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:DPH5(YLR172C)|FD-Score:4.97|P-value:3.29E-7||SGD DESC:Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm Gene:EHT1(YBR177C)|FD-Score:-4.26|P-value:1.04E-5||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication Gene:EST1(YLR233C)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:TLC1 RNA-associated factor involved in telomere length regulation as recruitment subunit of telomerase; has G-quadruplex promoting activity that is required for telomere elongation; possible role in activating telomere-bound Est2p-TLC1-RNA Gene:GCR2(YNL199C)|FD-Score:3.52|P-value:2.20E-4||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GCY1(YOR120W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GLR1(YPL091W)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GRE1(YPL223C)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Hydrophilin essential in dessication-rehydration process; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway Gene:GTT3(YEL017W)|FD-Score:3.79|P-value:7.41E-5||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:GUT1(YHL032C)|FD-Score:-5.04|P-value:2.32E-7||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:GYP7(YDL234C)|FD-Score:-4.35|P-value:6.91E-6||SGD DESC:GTPase-activating protein for yeast Rab family members including: Ypt7p (most effective), Ypt1p, Ypt31p, and Ypt32p (in vitro); involved in vesicle mediated protein trafficking Gene:IRC11(YOR013W_d)|FD-Score:3.79|P-value:7.55E-5||SGD DESC:Dubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci Gene:JEM1(YJL073W)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:KIP3(YGL216W)|FD-Score:-3.94|P-value:4.03E-5||SGD DESC:Kinesin-related motor protein involved in mitotic spindle positioning Gene:LSB3(YFR024C-A)|FD-Score:3.1|P-value:9.56E-4||SGD DESC:Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication Gene:LSB5(YCL034W)|FD-Score:8.02|P-value:5.28E-16||SGD DESC:Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; may mediate disassembly of the Pan1 complex from the endocytic coat Gene:MCH5(YOR306C)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport Gene:MEP2(YNL142W)|FD-Score:-4.07|P-value:2.36E-5||SGD DESC:Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MET28(YIR017C)|FD-Score:3.77|P-value:8.23E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator in the Cbf1p-Met4p-Met28p complex, participates in the regulation of sulfur metabolism Gene:MFA2(YNL145W)|FD-Score:5.8|P-value:3.28E-9||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1 Gene:MNN2(YBR015C)|FD-Score:7.39|P-value:7.29E-14||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRH1(YDR033W)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Protein that localizes primarily to the plasma membrane; also found at the nuclear envelope; the authentic, non-tagged protein is detected in mitochondria in a phosphorylated state; MRH1 has a paralog, YRO2, that arose from the whole genome duplication Gene:MRPL22(YNL177C)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSA1(YOR066W)|FD-Score:-4.03|P-value:2.80E-5||SGD DESC:Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:NEW1(YPL226W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:NHP10(YDL002C)|FD-Score:4.46|P-value:4.10E-6||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:PDR3(YBL005W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome; PDR3 has a paralog, PDR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:PET494(YNR045W)|FD-Score:-4.17|P-value:1.55E-5||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane Gene:PEX27(YOR193W)|FD-Score:-4.35|P-value:6.74E-6||SGD DESC:Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p Gene:PNP1(YLR209C)|FD-Score:3.98|P-value:3.43E-5||SGD DESC:Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway Gene:POR1(YNL055C)|FD-Score:-4.49|P-value:3.51E-6||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:PRY3(YJL078C)|FD-Score:3.43|P-value:2.96E-4||SGD DESC:Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p Gene:RPL35B(YDL136W)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPS29A(YLR388W)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:RPS9A(YPL081W)|FD-Score:5.72|P-value:5.21E-9||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9A has a paralog, RPS9B, that arose from the whole genome duplication Gene:SAC1(YKL212W)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SCO1(YBR037C)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication Gene:SCY1(YGL083W)|FD-Score:-3.8|P-value:7.16E-5||SGD DESC:Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase Gene:SFC1(YJR095W)|FD-Score:-3.57|P-value:1.77E-4||SGD DESC:Mitochondrial succinate-fumarate transporter, transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization Gene:SSA3(YBL075C)|FD-Score:3.76|P-value:8.58E-5||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:SWM1(YDR260C)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation Gene:TCB1(YOR086C)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TFB6(YOR352W)|FD-Score:4.45|P-value:4.36E-6||SGD DESC:Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YDR426C(YDR426C_d)|FD-Score:-3.34|P-value:4.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SNX41 Gene:YER119C-A(YER119C-A_d)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YER156C(YER156C_p)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 Gene:YGR149W(YGR149W_p)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Putative protein of unknown function; predicted to be an integal membrane protein Gene:YHC3(YJL059W)|FD-Score:4.77|P-value:9.31E-7||SGD DESC:Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) Gene:YHM2(YMR241W)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome Gene:YHR182W(YHR182W_p)|FD-Score:3.87|P-value:5.46E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YIP3(YNL044W)|FD-Score:-4.29|P-value:8.86E-6||SGD DESC:Protein localized to COPII vesicles, proposed to be involved in ER to Golgi transport; interacts with members of the Rab GTPase family and Yip1p; also interacts with Rtn1p Gene:YLR342W-A(YLR342W-A_p)|FD-Score:-3.11|P-value:9.42E-4||SGD DESC:Putative protein of unknown function Gene:YNR021W(YNR021W_p)|FD-Score:-3.37|P-value:3.70E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNR021W is not an essential gene Gene:YNR065C(YNR065C_p)|FD-Score:4.44|P-value:4.40E-6||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOR041C(YOR041C_d)|FD-Score:-6.28|P-value:1.72E-10||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance Gene:AIM19(YIL087C)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth Gene:ATP19(YOL077W-A)|FD-Score:-3.76|P-value:8.53E-5||SGD DESC:Subunit k of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase Gene:AVT4(YNL101W)|FD-Score:4.37|P-value:6.18E-6||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BTN2(YGR142W)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; BTN2 has a paralog, CUR1, that arose from the whole genome duplication Gene:CKI1(YLR133W)|FD-Score:-3.37|P-value:3.70E-4||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:DCI1(YOR180C)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed Gene:DOT6(YER088C)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:DPH5(YLR172C)|FD-Score:4.97|P-value:3.29E-7||SGD DESC:Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm Gene:EHT1(YBR177C)|FD-Score:-4.26|P-value:1.04E-5||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication Gene:EST1(YLR233C)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:TLC1 RNA-associated factor involved in telomere length regulation as recruitment subunit of telomerase; has G-quadruplex promoting activity that is required for telomere elongation; possible role in activating telomere-bound Est2p-TLC1-RNA Gene:GCR2(YNL199C)|FD-Score:3.52|P-value:2.20E-4||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GCY1(YOR120W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GLR1(YPL091W)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GRE1(YPL223C)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Hydrophilin essential in dessication-rehydration process; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway Gene:GTT3(YEL017W)|FD-Score:3.79|P-value:7.41E-5||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:GUT1(YHL032C)|FD-Score:-5.04|P-value:2.32E-7||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:GYP7(YDL234C)|FD-Score:-4.35|P-value:6.91E-6||SGD DESC:GTPase-activating protein for yeast Rab family members including: Ypt7p (most effective), Ypt1p, Ypt31p, and Ypt32p (in vitro); involved in vesicle mediated protein trafficking Gene:IRC11(YOR013W_d)|FD-Score:3.79|P-value:7.55E-5||SGD DESC:Dubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci Gene:JEM1(YJL073W)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:KIP3(YGL216W)|FD-Score:-3.94|P-value:4.03E-5||SGD DESC:Kinesin-related motor protein involved in mitotic spindle positioning Gene:LSB3(YFR024C-A)|FD-Score:3.1|P-value:9.56E-4||SGD DESC:Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication Gene:LSB5(YCL034W)|FD-Score:8.02|P-value:5.28E-16||SGD DESC:Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; may mediate disassembly of the Pan1 complex from the endocytic coat Gene:MCH5(YOR306C)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport Gene:MEP2(YNL142W)|FD-Score:-4.07|P-value:2.36E-5||SGD DESC:Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MET28(YIR017C)|FD-Score:3.77|P-value:8.23E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator in the Cbf1p-Met4p-Met28p complex, participates in the regulation of sulfur metabolism Gene:MFA2(YNL145W)|FD-Score:5.8|P-value:3.28E-9||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1 Gene:MNN2(YBR015C)|FD-Score:7.39|P-value:7.29E-14||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRH1(YDR033W)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Protein that localizes primarily to the plasma membrane; also found at the nuclear envelope; the authentic, non-tagged protein is detected in mitochondria in a phosphorylated state; MRH1 has a paralog, YRO2, that arose from the whole genome duplication Gene:MRPL22(YNL177C)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSA1(YOR066W)|FD-Score:-4.03|P-value:2.80E-5||SGD DESC:Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:NEW1(YPL226W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:NHP10(YDL002C)|FD-Score:4.46|P-value:4.10E-6||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:PDR3(YBL005W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome; PDR3 has a paralog, PDR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:PET494(YNR045W)|FD-Score:-4.17|P-value:1.55E-5||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane Gene:PEX27(YOR193W)|FD-Score:-4.35|P-value:6.74E-6||SGD DESC:Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p Gene:PNP1(YLR209C)|FD-Score:3.98|P-value:3.43E-5||SGD DESC:Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway Gene:POR1(YNL055C)|FD-Score:-4.49|P-value:3.51E-6||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:PRY3(YJL078C)|FD-Score:3.43|P-value:2.96E-4||SGD DESC:Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p Gene:RPL35B(YDL136W)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPS29A(YLR388W)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:RPS9A(YPL081W)|FD-Score:5.72|P-value:5.21E-9||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9A has a paralog, RPS9B, that arose from the whole genome duplication Gene:SAC1(YKL212W)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SCO1(YBR037C)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication Gene:SCY1(YGL083W)|FD-Score:-3.8|P-value:7.16E-5||SGD DESC:Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase Gene:SFC1(YJR095W)|FD-Score:-3.57|P-value:1.77E-4||SGD DESC:Mitochondrial succinate-fumarate transporter, transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization Gene:SSA3(YBL075C)|FD-Score:3.76|P-value:8.58E-5||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:SWM1(YDR260C)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation Gene:TCB1(YOR086C)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TFB6(YOR352W)|FD-Score:4.45|P-value:4.36E-6||SGD DESC:Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YDR426C(YDR426C_d)|FD-Score:-3.34|P-value:4.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SNX41 Gene:YER119C-A(YER119C-A_d)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YER156C(YER156C_p)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 Gene:YGR149W(YGR149W_p)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Putative protein of unknown function; predicted to be an integal membrane protein Gene:YHC3(YJL059W)|FD-Score:4.77|P-value:9.31E-7||SGD DESC:Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) Gene:YHM2(YMR241W)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome Gene:YHR182W(YHR182W_p)|FD-Score:3.87|P-value:5.46E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YIP3(YNL044W)|FD-Score:-4.29|P-value:8.86E-6||SGD DESC:Protein localized to COPII vesicles, proposed to be involved in ER to Golgi transport; interacts with members of the Rab GTPase family and Yip1p; also interacts with Rtn1p Gene:YLR342W-A(YLR342W-A_p)|FD-Score:-3.11|P-value:9.42E-4||SGD DESC:Putative protein of unknown function Gene:YNR021W(YNR021W_p)|FD-Score:-3.37|P-value:3.70E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNR021W is not an essential gene Gene:YNR065C(YNR065C_p)|FD-Score:4.44|P-value:4.40E-6||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOR041C(YOR041C_d)|FD-Score:-6.28|P-value:1.72E-10||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL182W5.511.76E-81.51FAS1Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
YPL217C4.003.18E-50.56BMS1GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p
YER082C3.442.89E-40.14UTP7Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YJL091C3.304.89E-40.12GWT1Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors
YPL210C3.177.59E-40.01SRP72Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane
YKR071C3.167.89E-40.01DRE2Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress
YPR177C_d3.158.04E-40.02YPR177C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex
YDL209C3.138.72E-40.03CWC2Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2
YIL118W3.109.77E-40.23RHO3Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p
YDL212W2.870.002040.00SHR3Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface
YLR166C2.870.002060.04SEC10Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion
YPR137W2.830.002300.04RRP9Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein
YKL172W2.790.002630.20EBP2Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
YCR052W2.600.004730.01RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YMR239C2.580.004870.09RNT1Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YCL034W8.025.28E-16LSB5Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; may mediate disassembly of the Pan1 complex from the endocytic coat
YBR015C7.397.29E-14MNN2Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment
YNL145W5.803.28E-9MFA2Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1
YPL081W5.725.21E-9RPS9AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9A has a paralog, RPS9B, that arose from the whole genome duplication
YLR172C4.973.29E-7DPH5Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm
YJL059W4.779.31E-7YHC3Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis)
YDL002C4.464.10E-6NHP10Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair
YOR352W4.454.36E-6TFB6Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
YNR065C_p4.444.40E-6YNR065C_pProtein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene
YNL101W4.376.18E-6AVT4Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
YER156C_p4.241.11E-5YER156C_pPutative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1
YKL212W4.211.28E-5SAC1Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
YDR260C4.141.72E-5SWM1Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation
YPL091W4.042.66E-5GLR1Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress
YLR209C3.983.43E-5PNP1Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway

GO enrichment analysis for SGTC_3301
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1285.46E-23SGTC_2261,3-diallylurea 8.3 mMMiscellaneous745370.0172414
0.0621.93E-6SGTC_1543389-1638 511.1 μMChemDiv (Drug-like library)52386860.0581395
0.0603.95E-6SGTC_21685727979 200.0 μMChembridge (Fragment library)6952100.0909091
0.0571.02E-5SGTC_27885282734 71.4 μMChembridge (Drug-like library)7437060.125
0.0525.50E-5SGTC_21785812956 200.0 μMChembridge (Fragment library)54033830.0694444
0.0517.71E-5SGTC_32419133421 49.5 μMChembridge (Drug-like library)170182210.0769231PDR1
0.0501.04E-4SGTC_2417cerulenin 830.0 nMICCB bioactive library52820540.0273973
0.0501.21E-4SGTC_1747st037496 66.6 μMTimTec (Natural product derivative library)7233050.0869565
0.0491.74E-4SGTC_500h-89 112.0 μMICCB bioactive library4492410.0813954cell wall signaling
0.0481.95E-4SGTC_8420271-0008 11.1 μMChemDiv (Drug-like library)12217090.0606061heme biosynthesis & mitochondrial translocase
0.0482.37E-4SGTC_1683st010134 710.0 nMTimTec (Natural product derivative library)38181340.0821918
0.0482.45E-4SGTC_20495262831 67.2 μMChembridge (Fragment library)7634170.114286
0.0482.45E-4SGTC_1963st076565 50.3 μMTimTec (Natural product derivative library)49142800.0537634
0.0482.50E-4SGTC_32209131269 49.5 μMChembridge (Drug-like library)177222060.155844
0.0472.70E-4SGTC_33399145883 14.9 μMChembridge (Drug-like library)20306700.189873

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3073911497249.47 μM0.72916741905075Chembridge (Drug-like library)272.368643.5750460S ribosome export
SGTC_3305912702753.83 μM0.58181841905295Chembridge (Drug-like library)316.378142.87506
SGTC_3300912461550.48 μM0.57692324824903Chembridge (Drug-like library)288.368043.07205
SGTC_3299912198243.13 μM0.56603817594604Chembridge (Drug-like library)273.35672.22105
SGTC_3102912166949.47 μM0.55769241905072Chembridge (Drug-like library)272.368643.57504
SGTC_3306912767659.79 μM0.55769241905287Chembridge (Drug-like library)264.369783.04205
SGTC_3292912082559.78 μM0.5471724824900Chembridge (Drug-like library)276.3325233.29405
SGTC_3101912053149.47 μM0.47457624824904Chembridge (Drug-like library)288.368043.27705
SGTC_3302912539516.35 μM0.46428617599668Chembridge (Drug-like library)284.379343.55704
SGTC_3116912494349.47 μM0.4561441905195Chembridge (Drug-like library)288.368042.92405