9133968

2-N-(4-ethylphenyl)-6-[[4-(4-methoxyphenyl)piperazin-1-yl]methyl]-1,3,5-triazine-2,4-diamine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3313
Screen concentration 57.0 μM
Source Chembridge (Drug-like library)
PubChem CID 1494643
SMILES CCC1=CC=C(C=C1)NC2=NC(=NC(=N2)N)CN3CCN(CC3)C4=CC=C(C=C4)OC
Standardized SMILES CCc1ccc(Nc2nc(N)nc(CN3CCN(CC3)c4ccc(OC)cc4)n2)cc1
Molecular weight 419.5227
ALogP 4.31
H-bond donor count 2
H-bond acceptor count 8
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.94
% growth inhibition (Hom. pool) 8.53


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1494643
Download HIP data (tab-delimited text)  (excel)
Gene:CDC16(YKL022C)|FD-Score:3.32|P-value:4.44E-4|Clearance:0.05||SGD DESC:Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation Gene:DIB1(YPR082C)|FD-Score:-4.99|P-value:3.01E-7|Clearance:0||SGD DESC:17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein Gene:EBP2(YKL172W)|FD-Score:-4.53|P-value:2.89E-6|Clearance:0||SGD DESC:Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering Gene:HSP10(YOR020C)|FD-Score:3.28|P-value:5.24E-4|Clearance:0.16||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:LSM5(YER146W)|FD-Score:3.64|P-value:1.38E-4|Clearance:0.31||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MIA40(YKL195W)|FD-Score:-3.12|P-value:9.11E-4|Clearance:0||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NHP2(YDL208W)|FD-Score:-3.38|P-value:3.62E-4|Clearance:0||SGD DESC:Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing Gene:SAM50(YNL026W)|FD-Score:-4.17|P-value:1.53E-5|Clearance:0||SGD DESC:Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 Gene:SGD1(YLR336C)|FD-Score:3.11|P-value:9.26E-4|Clearance:0.05||SGD DESC:Essential nuclear protein, required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia Gene:YPR177C(YPR177C_d)|FD-Score:-3.79|P-value:7.54E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex Gene:CDC16(YKL022C)|FD-Score:3.32|P-value:4.44E-4|Clearance:0.05||SGD DESC:Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation Gene:DIB1(YPR082C)|FD-Score:-4.99|P-value:3.01E-7|Clearance:0||SGD DESC:17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein Gene:EBP2(YKL172W)|FD-Score:-4.53|P-value:2.89E-6|Clearance:0||SGD DESC:Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering Gene:HSP10(YOR020C)|FD-Score:3.28|P-value:5.24E-4|Clearance:0.16||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:LSM5(YER146W)|FD-Score:3.64|P-value:1.38E-4|Clearance:0.31||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MIA40(YKL195W)|FD-Score:-3.12|P-value:9.11E-4|Clearance:0||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NHP2(YDL208W)|FD-Score:-3.38|P-value:3.62E-4|Clearance:0||SGD DESC:Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing Gene:SAM50(YNL026W)|FD-Score:-4.17|P-value:1.53E-5|Clearance:0||SGD DESC:Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 Gene:SGD1(YLR336C)|FD-Score:3.11|P-value:9.26E-4|Clearance:0.05||SGD DESC:Essential nuclear protein, required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia Gene:YPR177C(YPR177C_d)|FD-Score:-3.79|P-value:7.54E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1494643
Download HOP data (tab-delimited text)  (excel)
Gene:BNI5(YNL166C)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner Gene:CLA4(YNL298W)|FD-Score:-3.37|P-value:3.75E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CYB2(YML054C)|FD-Score:-4.04|P-value:2.66E-5||SGD DESC:Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions Gene:CYC2(YOR037W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p) Gene:DAN1(YJR150C)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:DUS4(YLR405W)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:ECM29(YHL030W)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:EMP70(YLR083C)|FD-Score:-4.44|P-value:4.52E-6||SGD DESC:Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments Gene:FBP1(YLR377C)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p Gene:FMS1(YMR020W)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Polyamine oxidase, converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis Gene:FRE2(YKL220C)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels Gene:GAL11(YOL051W)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:HXK1(YFR053C)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:KTR4(YBR199W)|FD-Score:4.88|P-value:5.29E-7||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family Gene:LYS21(YDL131W)|FD-Score:3.85|P-value:6.01E-5||SGD DESC:Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication Gene:MRP7(YNL005C)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NDT80(YHR124W)|FD-Score:-3.23|P-value:6.21E-4||SGD DESC:Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) Gene:POX1(YGL205W)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix Gene:REX2(YLR059C)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease Gene:RMT2(YDR465C)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress Gene:RPL21A(YBR191W)|FD-Score:-3.26|P-value:5.59E-4||SGD DESC:Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication Gene:SNN1(YNL086W_p)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to endosomes Gene:SNT2(YGL131C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:STO1(YMR125W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SUB1(YMR039C)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress Gene:SWI3(YJL176C)|FD-Score:-4.02|P-value:2.90E-5||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:TDA4(YJR116W_p)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele Gene:YBR090C(YBR090C_p)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YBR242W(YBR242W_p)|FD-Score:-3.72|P-value:9.95E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YBR292C(YBR292C_d)|FD-Score:-3.3|P-value:4.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene Gene:YCL076W(YCL076W_d)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL183C(YDL183C)|FD-Score:-3.17|P-value:7.56E-4||SGD DESC:Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene Gene:YDR115W(YDR115W_p)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress Gene:YDR306C(YDR306C_p)|FD-Score:4.76|P-value:9.78E-7||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YHR139C-A(YHR139C-A_d)|FD-Score:3.87|P-value:5.36E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR182W(YHR182W_p)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YIL058W(YIL058W_d)|FD-Score:7.47|P-value:3.96E-14||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL160C(YJL160C_p)|FD-Score:-3.21|P-value:6.68E-4||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YJR128W(YJR128W_d)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RSF2 Gene:YKR078W(YKR078W)|FD-Score:-3.32|P-value:4.56E-4||SGD DESC:Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:YOL160W(YOL160W_d)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL068C(YPL068C_p)|FD-Score:4.42|P-value:4.88E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS Gene:BNI5(YNL166C)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner Gene:CLA4(YNL298W)|FD-Score:-3.37|P-value:3.75E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CYB2(YML054C)|FD-Score:-4.04|P-value:2.66E-5||SGD DESC:Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions Gene:CYC2(YOR037W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p) Gene:DAN1(YJR150C)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:DUS4(YLR405W)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:ECM29(YHL030W)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:EMP70(YLR083C)|FD-Score:-4.44|P-value:4.52E-6||SGD DESC:Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments Gene:FBP1(YLR377C)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p Gene:FMS1(YMR020W)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Polyamine oxidase, converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis Gene:FRE2(YKL220C)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels Gene:GAL11(YOL051W)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:HXK1(YFR053C)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:KTR4(YBR199W)|FD-Score:4.88|P-value:5.29E-7||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family Gene:LYS21(YDL131W)|FD-Score:3.85|P-value:6.01E-5||SGD DESC:Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication Gene:MRP7(YNL005C)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NDT80(YHR124W)|FD-Score:-3.23|P-value:6.21E-4||SGD DESC:Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) Gene:POX1(YGL205W)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix Gene:REX2(YLR059C)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease Gene:RMT2(YDR465C)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress Gene:RPL21A(YBR191W)|FD-Score:-3.26|P-value:5.59E-4||SGD DESC:Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication Gene:SNN1(YNL086W_p)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to endosomes Gene:SNT2(YGL131C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:STO1(YMR125W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SUB1(YMR039C)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress Gene:SWI3(YJL176C)|FD-Score:-4.02|P-value:2.90E-5||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:TDA4(YJR116W_p)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele Gene:YBR090C(YBR090C_p)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YBR242W(YBR242W_p)|FD-Score:-3.72|P-value:9.95E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YBR292C(YBR292C_d)|FD-Score:-3.3|P-value:4.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene Gene:YCL076W(YCL076W_d)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL183C(YDL183C)|FD-Score:-3.17|P-value:7.56E-4||SGD DESC:Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene Gene:YDR115W(YDR115W_p)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress Gene:YDR306C(YDR306C_p)|FD-Score:4.76|P-value:9.78E-7||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YHR139C-A(YHR139C-A_d)|FD-Score:3.87|P-value:5.36E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR182W(YHR182W_p)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YIL058W(YIL058W_d)|FD-Score:7.47|P-value:3.96E-14||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL160C(YJL160C_p)|FD-Score:-3.21|P-value:6.68E-4||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YJR128W(YJR128W_d)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RSF2 Gene:YKR078W(YKR078W)|FD-Score:-3.32|P-value:4.56E-4||SGD DESC:Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:YOL160W(YOL160W_d)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL068C(YPL068C_p)|FD-Score:4.42|P-value:4.88E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YER146W3.641.38E-40.31LSM5Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
YKL022C3.324.44E-40.05CDC16Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation
YOR020C3.285.24E-40.17HSP10Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES
YLR336C3.119.26E-40.05SGD1Essential nuclear protein, required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia
YPL016W3.060.001100.05SWI1Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer
YJL085W3.020.001280.03EXO70Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
YOL034W2.990.001390.19SMC5Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair
YER043C2.800.002530.06SAH1S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor
YHR062C2.750.003010.12RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YMR112C2.630.004260.03MED11Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein
YNL103W2.600.004610.07MET4Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p
YNL232W2.530.005720.00CSL4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1)
YKR068C2.530.005770.00BET3Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex
YJL104W2.520.005850.03PAM16Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain
YDR413C_d2.490.006460.00YDR413C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIL058W_d7.473.96E-14YIL058W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YBR199W4.885.29E-7KTR4Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family
YDR306C_p4.769.78E-7YDR306C_pF-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain
YPL068C_p4.424.88E-6YPL068C_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS
YOL051W4.191.42E-5GAL11Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator
YBR090C_p4.022.89E-5YBR090C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
YHR139C-A_d3.875.36E-5YHR139C-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDL131W3.856.01E-5LYS21Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication
YGL131C3.681.16E-4SNT2DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress
YMR125W3.631.41E-4STO1Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80
YGL205W3.482.54E-4POX1Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix
YOR037W3.472.59E-4CYC2Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
YNL005C3.423.18E-4MRP7Mitochondrial ribosomal protein of the large subunit
YJR116W_p3.383.68E-4TDA4_pPutative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele
YCL076W_d3.363.94E-4YCL076W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_3313
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1527.13E-32SGTC_33149135860 59.4 μMChembridge (Drug-like library)252524780.120482
0.0849.53E-11SGTC_32979117502 71.4 μMChembridge (Drug-like library)74537200.121951
0.0815.27E-10SGTC_1793st048256 72.2 μMTimTec (Natural product derivative library)6720220.182927
0.0782.22E-9SGTC_1563conessine 56.1 μMTimTec (Pure natural product library)4410820.0537634
0.0773.38E-9SGTC_1924st058459 75.1 μMTimTec (Natural product derivative library)6886830.144737
0.0764.66E-9SGTC_20765212012 200.0 μMChembridge (Fragment library)6048020.0779221
0.0691.23E-7SGTC_28989056270 58.4 μMChembridge (Drug-like library)191546990.0978261
0.0663.69E-7SGTC_1938st074717 56.4 μMTimTec (Natural product derivative library)172509790.127907
0.0665.02E-7SGTC_9963970-0799 1.8 μMChemDiv (Drug-like library)66156480.16
0.0561.45E-5SGTC_28869058944 52.0 μMChembridge (Drug-like library)49499290.225352
0.0543.23E-5SGTC_1630st005209 31.3 μMTimTec (Natural product derivative library)33673140.0769231
0.0543.63E-5SGTC_33549151612 71.4 μMChembridge (Drug-like library)272619490.116279
0.0526.07E-5SGTC_33129133330 71.4 μMChembridge (Drug-like library)176173240.0617284
0.0526.75E-5SGTC_28999053889 58.4 μMChembridge (Drug-like library)64700520.146341
0.0527.22E-5SGTC_9983996-0085 177.0 μMChemDiv (Drug-like library)7153910.1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2951907295058.06 μM0.64062516488155Chembridge (Drug-like library)425.914564.0328ERG2
SGTC_3130912741449.47 μM0.5606061494646Chembridge (Drug-like library)425.914564.0328
SGTC_7064262-005746.9 μM0.362069883298ChemDiv (Drug-like library)338.48644.71903fatty acid desaturase (OLE1)
SGTC_3005907649671.43 μM0.33333321368410Chembridge (Drug-like library)310.390183.67713
SGTC_14554262-0120131 μM0.3157891529587ChemDiv (Drug-like library)308.460424.87302plasma membrane duress
SGTC_2784092-082123.9 μM0.310345877900ChemDiv (Drug-like library)328.87895.05102plasma membrane duress
SGTC_20505263056171 μM0.290323763468Chembridge (Fragment library)268.35353.29113fatty acid desaturase (OLE1)
SGTC_3084092-1169104 μM0.2898552268590ChemDiv (Drug-like library)346.851123.8514plasma membrane duress
SGTC_5423381-024489.3 μM0.2876711112843ChemDiv (Drug-like library)360.448863.67603
SGTC_3315913639568.55 μM0.287516876844Chembridge (Drug-like library)423.960043.90928