9135860

1-(3-butoxy-4-chlorophenyl)sulfonyl-4-methylpiperazine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3314
Screen concentration 59.4 μM
Source Chembridge (Drug-like library)
PubChem CID 25252478
SMILES CCCCOC1=C(C=CC(=C1)S(=O)(=O)N2CCN(CC2)C)Cl
Standardized SMILES CCCCOc1cc(ccc1Cl)S(=O)(=O)N2CCN(C)CC2
Molecular weight 346.8727
ALogP 2.88
H-bond donor count 0
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.65
% growth inhibition (Hom. pool) 7.56


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 25252478
Download HIP data (tab-delimited text)  (excel)
Gene:DIB1(YPR082C)|FD-Score:-4.41|P-value:5.15E-6|Clearance:0||SGD DESC:17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein Gene:EBP2(YKL172W)|FD-Score:-4.82|P-value:7.22E-7|Clearance:0||SGD DESC:Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering Gene:EMG1(YLR186W)|FD-Score:-3.25|P-value:5.72E-4|Clearance:0||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:ESP1(YGR098C)|FD-Score:4.15|P-value:1.65E-5|Clearance:0.38||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:HIP1(YGR191W)|FD-Score:-3.91|P-value:4.57E-5|Clearance:0||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:HTS1(YPR033C)|FD-Score:3.53|P-value:2.12E-4|Clearance:0.35||SGD DESC:Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome Gene:NOP19(YGR251W)|FD-Score:3.17|P-value:7.55E-4|Clearance:0.05||SGD DESC:Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes Gene:NUS1(YDL193W)|FD-Score:-3.38|P-value:3.68E-4|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:OKP1(YGR179C)|FD-Score:3.54|P-value:1.98E-4|Clearance:0.02||SGD DESC:Outer kinetochore protein required for accurate chromosome segregation; component of COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) a kinetochore sub-complex which functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-Q and fission yeast fta7 Gene:PDS1(YDR113C)|FD-Score:-3.34|P-value:4.13E-4|Clearance:0||SGD DESC:Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:PRP6(YBR055C)|FD-Score:-4.11|P-value:1.97E-5|Clearance:0||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPB2(YOR151C)|FD-Score:-3.21|P-value:6.66E-4|Clearance:0||SGD DESC:RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit Gene:RPC40(YPR110C)|FD-Score:-5.42|P-value:2.98E-8|Clearance:0||SGD DESC:RNA polymerase subunit AC40, common to RNA polymerase I and III Gene:RRB1(YMR131C)|FD-Score:3.12|P-value:8.92E-4|Clearance:0.09||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:RRP14(YKL082C)|FD-Score:3.78|P-value:7.96E-5|Clearance:0.08||SGD DESC:Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family Gene:SAD1(YFR005C)|FD-Score:-3.49|P-value:2.40E-4|Clearance:0||SGD DESC:Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle Gene:SFT1(YKL006C-A)|FD-Score:3.7|P-value:1.08E-4|Clearance:0.16||SGD DESC:Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment Gene:SOG2(YOR353C)|FD-Score:-3.38|P-value:3.62E-4|Clearance:0||SGD DESC:Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis Gene:TFA2(YKR062W)|FD-Score:-3.28|P-value:5.22E-4|Clearance:0||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:TTI1(YKL033W)|FD-Score:-3.27|P-value:5.45E-4|Clearance:0||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies Gene:DIB1(YPR082C)|FD-Score:-4.41|P-value:5.15E-6|Clearance:0||SGD DESC:17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein Gene:EBP2(YKL172W)|FD-Score:-4.82|P-value:7.22E-7|Clearance:0||SGD DESC:Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering Gene:EMG1(YLR186W)|FD-Score:-3.25|P-value:5.72E-4|Clearance:0||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:ESP1(YGR098C)|FD-Score:4.15|P-value:1.65E-5|Clearance:0.38||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:HIP1(YGR191W)|FD-Score:-3.91|P-value:4.57E-5|Clearance:0||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:HTS1(YPR033C)|FD-Score:3.53|P-value:2.12E-4|Clearance:0.35||SGD DESC:Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome Gene:NOP19(YGR251W)|FD-Score:3.17|P-value:7.55E-4|Clearance:0.05||SGD DESC:Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes Gene:NUS1(YDL193W)|FD-Score:-3.38|P-value:3.68E-4|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:OKP1(YGR179C)|FD-Score:3.54|P-value:1.98E-4|Clearance:0.02||SGD DESC:Outer kinetochore protein required for accurate chromosome segregation; component of COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) a kinetochore sub-complex which functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-Q and fission yeast fta7 Gene:PDS1(YDR113C)|FD-Score:-3.34|P-value:4.13E-4|Clearance:0||SGD DESC:Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:PRP6(YBR055C)|FD-Score:-4.11|P-value:1.97E-5|Clearance:0||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPB2(YOR151C)|FD-Score:-3.21|P-value:6.66E-4|Clearance:0||SGD DESC:RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit Gene:RPC40(YPR110C)|FD-Score:-5.42|P-value:2.98E-8|Clearance:0||SGD DESC:RNA polymerase subunit AC40, common to RNA polymerase I and III Gene:RRB1(YMR131C)|FD-Score:3.12|P-value:8.92E-4|Clearance:0.09||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:RRP14(YKL082C)|FD-Score:3.78|P-value:7.96E-5|Clearance:0.08||SGD DESC:Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family Gene:SAD1(YFR005C)|FD-Score:-3.49|P-value:2.40E-4|Clearance:0||SGD DESC:Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle Gene:SFT1(YKL006C-A)|FD-Score:3.7|P-value:1.08E-4|Clearance:0.16||SGD DESC:Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment Gene:SOG2(YOR353C)|FD-Score:-3.38|P-value:3.62E-4|Clearance:0||SGD DESC:Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis Gene:TFA2(YKR062W)|FD-Score:-3.28|P-value:5.22E-4|Clearance:0||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:TTI1(YKL033W)|FD-Score:-3.27|P-value:5.45E-4|Clearance:0||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 25252478
Download HOP data (tab-delimited text)  (excel)
Gene:ACE2(YLR131C)|FD-Score:5.08|P-value:1.88E-7||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AFT1(YGL071W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARA2(YMR041C)|FD-Score:-3.11|P-value:9.32E-4||SGD DESC:NAD-dependent arabinose dehydrogenase, involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase Gene:ATG8(YBL078C)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:BCK2(YER167W)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:BTS1(YPL069C)|FD-Score:6.68|P-value:1.23E-11||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CAF130(YGR134W)|FD-Score:6.14|P-value:4.24E-10||SGD DESC:Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation Gene:CAR2(YLR438W)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress Gene:COG1(YGL223C)|FD-Score:-3.6|P-value:1.56E-4||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG5(YNL051W)|FD-Score:-3.16|P-value:7.83E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAP1(YPL170W)|FD-Score:-3.27|P-value:5.30E-4||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DYN1(YKR054C)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p Gene:ECM29(YHL030W)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:EMP70(YLR083C)|FD-Score:-4.26|P-value:1.00E-5||SGD DESC:Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments Gene:ERS1(YCR075C)|FD-Score:4.07|P-value:2.40E-5||SGD DESC:Protein with similarity to human cystinosin, which is a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains Gene:FRT1(YOR324C)|FD-Score:3.78|P-value:7.69E-5||SGD DESC:Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication Gene:GID7(YCL039W)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions Gene:HHF1(YBR009C)|FD-Score:4.02|P-value:2.90E-5||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HMG1(YML075C)|FD-Score:3.96|P-value:3.74E-5||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:LYS21(YDL131W)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication Gene:MDM1(YML104C)|FD-Score:3.14|P-value:8.39E-4||SGD DESC:Intermediate filament protein, required for nuclear and mitochondrial transmission to daughter buds; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:MIG3(YER028C)|FD-Score:4.3|P-value:8.63E-6||SGD DESC:Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication Gene:MRP17(YKL003C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator Gene:MSC3(YLR219W)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress Gene:OCA6(YDR067C_p)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:OPI3(YJR073C)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PPG1(YNR032W)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:Putative serine/threonine protein phosphatase of the type 2A-like phosphatase family, required for glycogen accumulation; interacts with Tap42p, which binds to and regulates other protein phosphatases Gene:PRR1(YKL116C)|FD-Score:3.81|P-value:6.99E-5||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor Gene:RAX2(YLR084C)|FD-Score:-3.14|P-value:8.39E-4||SGD DESC:N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p Gene:RKR1(YMR247C)|FD-Score:5.66|P-value:7.68E-9||SGD DESC:RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice Gene:RMD9(YGL107C)|FD-Score:-4.43|P-value:4.78E-6||SGD DESC:Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes Gene:RMT2(YDR465C)|FD-Score:-3.91|P-value:4.66E-5||SGD DESC:Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress Gene:RNQ1(YCL028W)|FD-Score:3.73|P-value:9.68E-5||SGD DESC:[PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate Gene:RPS17A(YML024W)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:SAC1(YKL212W)|FD-Score:4|P-value:3.19E-5||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAS3(YBL052C)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SIP4(YJL089W)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SMA2(YML066C)|FD-Score:3.88|P-value:5.32E-5||SGD DESC:Meiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation Gene:SNA4(YDL123W)|FD-Score:-3.12|P-value:8.97E-4||SGD DESC:Protein of unknown function, localized to the vacuolar outer membrane; predicted to be palmitoylated Gene:SNF7(YLR025W)|FD-Score:3.88|P-value:5.23E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SSE1(YPL106C)|FD-Score:3.87|P-value:5.36E-5||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:STB6(YKL072W)|FD-Score:3.11|P-value:9.22E-4||SGD DESC:Protein that binds Sin3p in a two-hybrid assay Gene:SYT1(YPR095C)|FD-Score:7.62|P-value:1.23E-14||SGD DESC:Guanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain Gene:TLG2(YOL018C)|FD-Score:-3.2|P-value:6.82E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOM7(YNL070W)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TRP1(YDR007W)|FD-Score:-3.86|P-value:5.64E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:UBR2(YLR024C)|FD-Score:4.75|P-value:1.03E-6||SGD DESC:Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex Gene:URA4(YLR420W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate Gene:UTR1(YJR049C)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VOA1(YGR106C)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:VPS45(YGL095C)|FD-Score:5.93|P-value:1.49E-9||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS63(YLR261C_d)|FD-Score:-4.18|P-value:1.44E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YCL076W(YCL076W_d)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL009C(YDL009C_p)|FD-Score:4.03|P-value:2.75E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene Gene:YDR034W-B(YDR034W-B_p)|FD-Score:-3.97|P-value:3.62E-5||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR431W(YDR431W_d)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL041W-A(YFL041W-A_p)|FD-Score:-3.27|P-value:5.44E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGR079W(YGR079W_p)|FD-Score:3.83|P-value:6.39E-5||SGD DESC:Putative protein of unknown function; YGR079W is not an essential gene Gene:YHR139C-A(YHR139C-A_d)|FD-Score:6.1|P-value:5.45E-10||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR182W(YHR182W_p)|FD-Score:-4.27|P-value:9.76E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YKL100C(YKL100C_p)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Putative protein of unknown function with similarity to a human minor histocompatibility antigen and signal peptide peptidases; YKL100C is not an essential gene Gene:YKL107W(YKL107W_p)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Putative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein Gene:YML116W-A(YML116W-A_d)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:Putative protein of unknown function Gene:YMR242W-A(YMR242W-A_p)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Putative protein of unknown function Gene:YOR225W(YOR225W_d)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR012W(YPR012W_d)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene Gene:YPR1(YDR368W)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:NADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication Gene:YPR153W(YPR153W)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Putative protein of unknown function Gene:ZRG8(YER033C)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency Gene:ACE2(YLR131C)|FD-Score:5.08|P-value:1.88E-7||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AFT1(YGL071W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARA2(YMR041C)|FD-Score:-3.11|P-value:9.32E-4||SGD DESC:NAD-dependent arabinose dehydrogenase, involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase Gene:ATG8(YBL078C)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:BCK2(YER167W)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:BTS1(YPL069C)|FD-Score:6.68|P-value:1.23E-11||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CAF130(YGR134W)|FD-Score:6.14|P-value:4.24E-10||SGD DESC:Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation Gene:CAR2(YLR438W)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress Gene:COG1(YGL223C)|FD-Score:-3.6|P-value:1.56E-4||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG5(YNL051W)|FD-Score:-3.16|P-value:7.83E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAP1(YPL170W)|FD-Score:-3.27|P-value:5.30E-4||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DYN1(YKR054C)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p Gene:ECM29(YHL030W)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:EMP70(YLR083C)|FD-Score:-4.26|P-value:1.00E-5||SGD DESC:Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments Gene:ERS1(YCR075C)|FD-Score:4.07|P-value:2.40E-5||SGD DESC:Protein with similarity to human cystinosin, which is a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains Gene:FRT1(YOR324C)|FD-Score:3.78|P-value:7.69E-5||SGD DESC:Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication Gene:GID7(YCL039W)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions Gene:HHF1(YBR009C)|FD-Score:4.02|P-value:2.90E-5||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HMG1(YML075C)|FD-Score:3.96|P-value:3.74E-5||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:LYS21(YDL131W)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication Gene:MDM1(YML104C)|FD-Score:3.14|P-value:8.39E-4||SGD DESC:Intermediate filament protein, required for nuclear and mitochondrial transmission to daughter buds; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:MIG3(YER028C)|FD-Score:4.3|P-value:8.63E-6||SGD DESC:Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication Gene:MRP17(YKL003C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator Gene:MSC3(YLR219W)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress Gene:OCA6(YDR067C_p)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:OPI3(YJR073C)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PPG1(YNR032W)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:Putative serine/threonine protein phosphatase of the type 2A-like phosphatase family, required for glycogen accumulation; interacts with Tap42p, which binds to and regulates other protein phosphatases Gene:PRR1(YKL116C)|FD-Score:3.81|P-value:6.99E-5||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor Gene:RAX2(YLR084C)|FD-Score:-3.14|P-value:8.39E-4||SGD DESC:N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p Gene:RKR1(YMR247C)|FD-Score:5.66|P-value:7.68E-9||SGD DESC:RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice Gene:RMD9(YGL107C)|FD-Score:-4.43|P-value:4.78E-6||SGD DESC:Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes Gene:RMT2(YDR465C)|FD-Score:-3.91|P-value:4.66E-5||SGD DESC:Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress Gene:RNQ1(YCL028W)|FD-Score:3.73|P-value:9.68E-5||SGD DESC:[PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate Gene:RPS17A(YML024W)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:SAC1(YKL212W)|FD-Score:4|P-value:3.19E-5||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAS3(YBL052C)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SIP4(YJL089W)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SMA2(YML066C)|FD-Score:3.88|P-value:5.32E-5||SGD DESC:Meiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation Gene:SNA4(YDL123W)|FD-Score:-3.12|P-value:8.97E-4||SGD DESC:Protein of unknown function, localized to the vacuolar outer membrane; predicted to be palmitoylated Gene:SNF7(YLR025W)|FD-Score:3.88|P-value:5.23E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SSE1(YPL106C)|FD-Score:3.87|P-value:5.36E-5||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:STB6(YKL072W)|FD-Score:3.11|P-value:9.22E-4||SGD DESC:Protein that binds Sin3p in a two-hybrid assay Gene:SYT1(YPR095C)|FD-Score:7.62|P-value:1.23E-14||SGD DESC:Guanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain Gene:TLG2(YOL018C)|FD-Score:-3.2|P-value:6.82E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOM7(YNL070W)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TRP1(YDR007W)|FD-Score:-3.86|P-value:5.64E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:UBR2(YLR024C)|FD-Score:4.75|P-value:1.03E-6||SGD DESC:Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex Gene:URA4(YLR420W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate Gene:UTR1(YJR049C)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VOA1(YGR106C)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:VPS45(YGL095C)|FD-Score:5.93|P-value:1.49E-9||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS63(YLR261C_d)|FD-Score:-4.18|P-value:1.44E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YCL076W(YCL076W_d)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL009C(YDL009C_p)|FD-Score:4.03|P-value:2.75E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene Gene:YDR034W-B(YDR034W-B_p)|FD-Score:-3.97|P-value:3.62E-5||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR431W(YDR431W_d)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL041W-A(YFL041W-A_p)|FD-Score:-3.27|P-value:5.44E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGR079W(YGR079W_p)|FD-Score:3.83|P-value:6.39E-5||SGD DESC:Putative protein of unknown function; YGR079W is not an essential gene Gene:YHR139C-A(YHR139C-A_d)|FD-Score:6.1|P-value:5.45E-10||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR182W(YHR182W_p)|FD-Score:-4.27|P-value:9.76E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YKL100C(YKL100C_p)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Putative protein of unknown function with similarity to a human minor histocompatibility antigen and signal peptide peptidases; YKL100C is not an essential gene Gene:YKL107W(YKL107W_p)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Putative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein Gene:YML116W-A(YML116W-A_d)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:Putative protein of unknown function Gene:YMR242W-A(YMR242W-A_p)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Putative protein of unknown function Gene:YOR225W(YOR225W_d)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR012W(YPR012W_d)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene Gene:YPR1(YDR368W)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:NADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication Gene:YPR153W(YPR153W)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Putative protein of unknown function Gene:ZRG8(YER033C)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR098C4.151.65E-50.38ESP1Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress
YKL082C3.787.96E-50.08RRP14Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family
YKL006C-A3.701.08E-40.16SFT1Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment
YGR179C3.541.98E-40.02OKP1Outer kinetochore protein required for accurate chromosome segregation; component of COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) a kinetochore sub-complex which functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-Q and fission yeast fta7
YPR033C3.532.12E-40.35HTS1Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome
YGR251W3.177.55E-40.05NOP19Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes
YMR131C3.128.92E-40.09RRB1Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p
YBR243C3.030.001210.02ALG7UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
YHR072W3.010.001310.34ERG7Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis
YKL108W2.670.003850.01SLD2Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress
YDR413C_d2.660.003920.01YDR413C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing
YDL084W2.650.004040.04SUB2Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress
YPL043W2.610.004580.10NOP4Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)
YLR378C2.510.006100.01SEC61Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER
YML085C2.500.006210.02TUB1Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPR095C7.621.23E-14SYT1Guanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain
YPL069C6.681.23E-11BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YGR134W6.144.24E-10CAF130Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation
YHR139C-A_d6.105.45E-10YHR139C-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YGL095C5.931.49E-9VPS45Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
YMR247C5.667.68E-9RKR1RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice
YLR131C5.081.88E-7ACE2Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication
YLR024C4.751.03E-6UBR2Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex
YER028C4.308.63E-6MIG3Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication
YCL076W_d4.221.24E-5YCL076W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YCR075C4.072.40E-5ERS1Protein with similarity to human cystinosin, which is a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains
YDL009C_p4.032.75E-5YDL009C_pProtein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene
YBR009C4.022.90E-5HHF1Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity
YKL212W4.003.19E-5SAC1Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
YML075C3.963.74E-5HMG1HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress

GO enrichment analysis for SGTC_3314
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1723.27E-40SGTC_2647atorvastatin 60.2 μMMiscellaneous608230.0625azole & statin
0.1577.85E-34SGTC_2648atorvastatin 78.7 μMMiscellaneous608230.0625azole & statin
0.1527.13E-32SGTC_33139133968 57.0 μMChembridge (Drug-like library)14946430.120482
0.1224.70E-21SGTC_2753fluvastatin 17.1 μMMiscellaneous4461550.0674157azole & statin
0.0993.07E-14SGTC_1097itavastatin 7.2 μMNIH Clinical Collection52824510.0721649azole & statin
0.0896.35E-12SGTC_1086cerivastatin 2.2 μMNIH Clinical Collection236639920.076087azole & statin
0.0881.20E-11SGTC_830170-0301 39.0 μMChemDiv (Drug-like library)30897160.046875endomembrane recycling
0.0855.91E-11SGTC_1924st058459 75.1 μMTimTec (Natural product derivative library)6886830.0833333
0.0831.74E-10SGTC_1547harmine 94.2 μMMiscellaneous52809530.0869565ergosterol biosynthesis
0.0807.01E-10SGTC_24695655019 90.8 μMMiscellaneous27882390.0897436
0.0808.40E-10SGTC_482fipronil 114.0 μMICCB bioactive library33520.0897436
0.0782.32E-9SGTC_418lividomycin a 100.0 μMMiscellaneous723940.0337079
0.0773.55E-9SGTC_1638st008370 60.6 μMTimTec (Natural product derivative library)242071340.12ergosterol biosynthesis
0.0756.96E-9SGTC_2629bisabolol 100.0 μMMicrosource (Natural product library)60976210.0684932
0.0758.84E-9SGTC_14244048-1941 275.0 μMChemDiv (Drug-like library)X14240.0704225

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2825799809458.44 μM0.3478262987815Chembridge (Drug-like library)418.506543.07407fatty acid desaturase (OLE1)
SGTC_3354915161271.43 μM0.33846227261949Chembridge (Drug-like library)334.433183.78715
SGTC_1633st00566467.9 μM0.3214292063822TimTec (Natural product derivative library)208.253642.88503
SGTC_2874904390471.43 μM0.3166674463428Chembridge (Drug-like library)312.38460.9606
SGTC_3230913166549.47 μM0.31578915943720Chembridge (Drug-like library)257.349162.28904
SGTC_1632st00565090 μM0.2931032759605TimTec (Natural product derivative library)222.280223.34103
SGTC_253dyclonine31.25 μM0.2857143180Miscellaneous289.412484.05303amide catabolism
SGTC_2920798290415.77 μM0.2857146456808Chembridge (Drug-like library)329.7743233.51915
SGTC_14234048-01125.59 μM0.283784X1423ChemDiv (Drug-like library)425.004475.7840
SGTC_2814797748242.97 μM0.2835826456762Chembridge (Drug-like library)343.8009034.00615