9136639

5-methyl-7-(4-methylpiperazin-1-yl)-3-phenyl-6-prop-2-enylpyrazolo[1,5-a]pyrimidine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3316
Screen concentration 10.6 μM
Source Chembridge (Drug-like library)
PubChem CID 4903665
SMILES CC1=NC2=C(C=NN2C(=C1CC=C)N3CCN(CC3)C)C4=CC=CC=C4
Standardized SMILES CN1CCN(CC1)c2c(CC=C)c(C)nc3c(cnn23)c4ccccc4
Molecular weight 347.4567
ALogP 3.57
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 13.87
% growth inhibition (Hom. pool) 7.58


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4903665
Download HIP data (tab-delimited text)  (excel)
Gene:ARC15(YIL062C)|FD-Score:4.52|P-value:3.13E-6|Clearance:0.66||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity Gene:CCT5(YJR064W)|FD-Score:-3.4|P-value:3.39E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC42(YLR229C)|FD-Score:3.82|P-value:6.63E-5|Clearance:0||SGD DESC:Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins Gene:FAS1(YKL182W)|FD-Score:3.27|P-value:5.47E-4|Clearance:0.03||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:IRA1(YBR140C)|FD-Score:-5.37|P-value:4.00E-8|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LSM4(YER112W)|FD-Score:5.15|P-value:1.33E-7|Clearance:0.63||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:NEO1(YIL048W)|FD-Score:10.8|P-value:2.07E-27|Clearance:5.64||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NOP15(YNL110C)|FD-Score:-4.19|P-value:1.40E-5|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:POL5(YEL055C)|FD-Score:3.56|P-value:1.83E-4|Clearance:0.2||SGD DESC:DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA Gene:PRP24(YMR268C)|FD-Score:3.74|P-value:9.05E-5|Clearance:0.18||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:PRP38(YGR075C)|FD-Score:3.85|P-value:5.81E-5|Clearance:0.03||SGD DESC:Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly Gene:RPL32(YBL092W)|FD-Score:-3.44|P-value:2.92E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPS3(YNL178W)|FD-Score:-3.75|P-value:8.75E-5|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP43(YCR035C)|FD-Score:-3.12|P-value:8.98E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:SPT16(YGL207W)|FD-Score:3.82|P-value:6.77E-5|Clearance:0.07||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome Gene:THS1(YIL078W)|FD-Score:3.36|P-value:3.89E-4|Clearance:0.1||SGD DESC:Threonyl-tRNA synthetase, essential cytoplasmic protein Gene:YDR341C(YDR341C)|FD-Score:3.16|P-value:7.81E-4|Clearance:0.15||SGD DESC:Arginyl-tRNA synthetase, proposed to be cytoplasmic but the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR230W(YLR230W_d)|FD-Score:3.24|P-value:6.01E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity Gene:YOR146W(YOR146W_d)|FD-Score:-3.4|P-value:3.40E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:ARC15(YIL062C)|FD-Score:4.52|P-value:3.13E-6|Clearance:0.66||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity Gene:CCT5(YJR064W)|FD-Score:-3.4|P-value:3.39E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC42(YLR229C)|FD-Score:3.82|P-value:6.63E-5|Clearance:0||SGD DESC:Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins Gene:FAS1(YKL182W)|FD-Score:3.27|P-value:5.47E-4|Clearance:0.03||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:IRA1(YBR140C)|FD-Score:-5.37|P-value:4.00E-8|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LSM4(YER112W)|FD-Score:5.15|P-value:1.33E-7|Clearance:0.63||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:NEO1(YIL048W)|FD-Score:10.8|P-value:2.07E-27|Clearance:5.64||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NOP15(YNL110C)|FD-Score:-4.19|P-value:1.40E-5|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:POL5(YEL055C)|FD-Score:3.56|P-value:1.83E-4|Clearance:0.2||SGD DESC:DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA Gene:PRP24(YMR268C)|FD-Score:3.74|P-value:9.05E-5|Clearance:0.18||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:PRP38(YGR075C)|FD-Score:3.85|P-value:5.81E-5|Clearance:0.03||SGD DESC:Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly Gene:RPL32(YBL092W)|FD-Score:-3.44|P-value:2.92E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPS3(YNL178W)|FD-Score:-3.75|P-value:8.75E-5|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP43(YCR035C)|FD-Score:-3.12|P-value:8.98E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:SPT16(YGL207W)|FD-Score:3.82|P-value:6.77E-5|Clearance:0.07||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome Gene:THS1(YIL078W)|FD-Score:3.36|P-value:3.89E-4|Clearance:0.1||SGD DESC:Threonyl-tRNA synthetase, essential cytoplasmic protein Gene:YDR341C(YDR341C)|FD-Score:3.16|P-value:7.81E-4|Clearance:0.15||SGD DESC:Arginyl-tRNA synthetase, proposed to be cytoplasmic but the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR230W(YLR230W_d)|FD-Score:3.24|P-value:6.01E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity Gene:YOR146W(YOR146W_d)|FD-Score:-3.4|P-value:3.40E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4903665
Download HOP data (tab-delimited text)  (excel)
Gene:ACE2(YLR131C)|FD-Score:11|P-value:1.35E-28||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ARA2(YMR041C)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:NAD-dependent arabinose dehydrogenase, involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase Gene:ATG33(YLR356W)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Mitochondrial mitophagy-specific protein; required primarily for mitophagy induced at the post-log phase; not required for other types of selective autophagy or macroautophagy; conserved within fungi, but not in higher eukaryotes Gene:COQ2(YNR041C)|FD-Score:3.24|P-value:6.08E-4||SGD DESC:Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis Gene:DAL7(YIR031C)|FD-Score:6.45|P-value:5.55E-11||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAS1(YJL149W)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C Gene:DCW1(YKL046C)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Putative mannosidase, GPI-anchored membrane protein required for cell wall biosynthesis in bud formation;homologous to Dfg5p Gene:DOG1(YHR044C)|FD-Score:4.67|P-value:1.54E-6||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; paralogous to DOG2; member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed, in vivo substrate has not yet been identified Gene:DUS4(YLR405W)|FD-Score:3.99|P-value:3.25E-5||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:FMP48(YGR052W_p)|FD-Score:4.59|P-value:2.22E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FUN14(YAL008W)|FD-Score:6.23|P-value:2.34E-10||SGD DESC:Mitochondrial protein of unknown function Gene:GIP4(YAL031C)|FD-Score:3.82|P-value:6.68E-5||SGD DESC:Cytoplasmic Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate Gene:HBN1(YCL026C-B_p)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:HER1(YOR227W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Protein of unknown function required for proliferation or remodeling of the ER that is caused by overexpression of Hmg2p; may interact with ribosomes, based on co-purification experiments; HER1 has a paralog, GIP3, that arose from the whole genome duplication Gene:HPF1(YOL155C)|FD-Score:4.02|P-value:2.92E-5||SGD DESC:Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines Gene:HXK1(YFR053C)|FD-Score:3.78|P-value:7.76E-5||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:IRC10(YOL015W)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC18(YJL037W)|FD-Score:4.48|P-value:3.82E-6||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC6(YFR043C)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:ITR2(YOL103W)|FD-Score:3.16|P-value:7.78E-4||SGD DESC:Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively Gene:IZH1(YDR492W)|FD-Score:3.82|P-value:6.63E-5||SGD DESC:Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc Gene:MSC3(YLR219W)|FD-Score:4.59|P-value:2.21E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress Gene:MSS18(YPR134W)|FD-Score:5.16|P-value:1.23E-7||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:MST1(YKL194C)|FD-Score:-3.3|P-value:4.85E-4||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:MTC4(YBR255W)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:MUP1(YGR055W)|FD-Score:-4.12|P-value:1.86E-5||SGD DESC:High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake Gene:NEJ1(YLR265C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p Gene:PFA5(YDR459C)|FD-Score:-3.25|P-value:5.70E-4||SGD DESC:Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain Gene:PPH21(YDL134C)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication Gene:PTH2(YBL057C)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:RFM1(YOR279C)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RGT1(YKL038W)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:RNQ1(YCL028W)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:[PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate Gene:RTG2(YGL252C)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p Gene:RXT2(YBR095C)|FD-Score:-3.94|P-value:4.07E-5||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SAC1(YKL212W)|FD-Score:5.48|P-value:2.08E-8||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SDS23(YGL056C)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:One of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; involved in cell separation during budding; SDS23 has a paralog, SDS24, that arose from the whole genome duplication Gene:SKN7(YHR206W)|FD-Score:-3.1|P-value:9.62E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SMA2(YML066C)|FD-Score:-3.8|P-value:7.29E-5||SGD DESC:Meiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation Gene:SNF5(YBR289W)|FD-Score:-4.81|P-value:7.59E-7||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SPO77(YLR341W)|FD-Score:3.11|P-value:9.47E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:SVF1(YDR346C)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:TOP1(YOL006C)|FD-Score:-3.2|P-value:6.82E-4||SGD DESC:Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination Gene:TPO3(YPR156C)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication Gene:TRM10(YOL093W)|FD-Score:3.21|P-value:6.60E-4||SGD DESC:tRNA methyltransferase; methylates the N-1 position of guanosine in tRNAs; protein abundance increases in response to DNA replication stress Gene:UBP1(YDL122W)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:UBS1(YBR165W)|FD-Score:-3.77|P-value:8.25E-5||SGD DESC:Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity Gene:VPS74(YDR372C)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YBL028C(YBL028C)|FD-Score:3.98|P-value:3.41E-5||SGD DESC:Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:YBL039W-B(YBL039W-B_p)|FD-Score:4.42|P-value:4.96E-6||SGD DESC:Putative protein of unknown function Gene:YGL114W(YGL114W_p)|FD-Score:-3.15|P-value:8.30E-4||SGD DESC:Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters Gene:YGR050C(YGR050C_p)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGR168C(YGR168C_p)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Putative protein of unknown function; YGR168C is not an essential gene Gene:YKR073C(YKR073C_d)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR173W-A(YMR173W-A_d)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YNL194C(YNL194C)|FD-Score:-3.8|P-value:7.14E-5||SGD DESC:Integral membrane protein required for sporulation and plasma membrane sphingolipid content; has sequence similarity to SUR7 and FMP45; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; GFP-fusion protein is more abundant at MCCs (membrane compartment occupied by Can1) in the presence of glycerol and oleate Gene:YPL182C(YPL182C_d)|FD-Score:-3.31|P-value:4.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:YPR015C(YPR015C_p)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YRR1(YOR162C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:ZRG8(YER033C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency Gene:ACE2(YLR131C)|FD-Score:11|P-value:1.35E-28||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ARA2(YMR041C)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:NAD-dependent arabinose dehydrogenase, involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase Gene:ATG33(YLR356W)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Mitochondrial mitophagy-specific protein; required primarily for mitophagy induced at the post-log phase; not required for other types of selective autophagy or macroautophagy; conserved within fungi, but not in higher eukaryotes Gene:COQ2(YNR041C)|FD-Score:3.24|P-value:6.08E-4||SGD DESC:Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis Gene:DAL7(YIR031C)|FD-Score:6.45|P-value:5.55E-11||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAS1(YJL149W)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C Gene:DCW1(YKL046C)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Putative mannosidase, GPI-anchored membrane protein required for cell wall biosynthesis in bud formation;homologous to Dfg5p Gene:DOG1(YHR044C)|FD-Score:4.67|P-value:1.54E-6||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; paralogous to DOG2; member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed, in vivo substrate has not yet been identified Gene:DUS4(YLR405W)|FD-Score:3.99|P-value:3.25E-5||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:FMP48(YGR052W_p)|FD-Score:4.59|P-value:2.22E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FUN14(YAL008W)|FD-Score:6.23|P-value:2.34E-10||SGD DESC:Mitochondrial protein of unknown function Gene:GIP4(YAL031C)|FD-Score:3.82|P-value:6.68E-5||SGD DESC:Cytoplasmic Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate Gene:HBN1(YCL026C-B_p)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:HER1(YOR227W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Protein of unknown function required for proliferation or remodeling of the ER that is caused by overexpression of Hmg2p; may interact with ribosomes, based on co-purification experiments; HER1 has a paralog, GIP3, that arose from the whole genome duplication Gene:HPF1(YOL155C)|FD-Score:4.02|P-value:2.92E-5||SGD DESC:Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines Gene:HXK1(YFR053C)|FD-Score:3.78|P-value:7.76E-5||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:IRC10(YOL015W)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC18(YJL037W)|FD-Score:4.48|P-value:3.82E-6||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC6(YFR043C)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:ITR2(YOL103W)|FD-Score:3.16|P-value:7.78E-4||SGD DESC:Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively Gene:IZH1(YDR492W)|FD-Score:3.82|P-value:6.63E-5||SGD DESC:Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc Gene:MSC3(YLR219W)|FD-Score:4.59|P-value:2.21E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress Gene:MSS18(YPR134W)|FD-Score:5.16|P-value:1.23E-7||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:MST1(YKL194C)|FD-Score:-3.3|P-value:4.85E-4||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:MTC4(YBR255W)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:MUP1(YGR055W)|FD-Score:-4.12|P-value:1.86E-5||SGD DESC:High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake Gene:NEJ1(YLR265C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p Gene:PFA5(YDR459C)|FD-Score:-3.25|P-value:5.70E-4||SGD DESC:Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain Gene:PPH21(YDL134C)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication Gene:PTH2(YBL057C)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:RFM1(YOR279C)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RGT1(YKL038W)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:RNQ1(YCL028W)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:[PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate Gene:RTG2(YGL252C)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p Gene:RXT2(YBR095C)|FD-Score:-3.94|P-value:4.07E-5||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SAC1(YKL212W)|FD-Score:5.48|P-value:2.08E-8||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SDS23(YGL056C)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:One of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; involved in cell separation during budding; SDS23 has a paralog, SDS24, that arose from the whole genome duplication Gene:SKN7(YHR206W)|FD-Score:-3.1|P-value:9.62E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SMA2(YML066C)|FD-Score:-3.8|P-value:7.29E-5||SGD DESC:Meiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation Gene:SNF5(YBR289W)|FD-Score:-4.81|P-value:7.59E-7||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SPO77(YLR341W)|FD-Score:3.11|P-value:9.47E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:SVF1(YDR346C)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:TOP1(YOL006C)|FD-Score:-3.2|P-value:6.82E-4||SGD DESC:Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination Gene:TPO3(YPR156C)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication Gene:TRM10(YOL093W)|FD-Score:3.21|P-value:6.60E-4||SGD DESC:tRNA methyltransferase; methylates the N-1 position of guanosine in tRNAs; protein abundance increases in response to DNA replication stress Gene:UBP1(YDL122W)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:UBS1(YBR165W)|FD-Score:-3.77|P-value:8.25E-5||SGD DESC:Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity Gene:VPS74(YDR372C)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YBL028C(YBL028C)|FD-Score:3.98|P-value:3.41E-5||SGD DESC:Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:YBL039W-B(YBL039W-B_p)|FD-Score:4.42|P-value:4.96E-6||SGD DESC:Putative protein of unknown function Gene:YGL114W(YGL114W_p)|FD-Score:-3.15|P-value:8.30E-4||SGD DESC:Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters Gene:YGR050C(YGR050C_p)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGR168C(YGR168C_p)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Putative protein of unknown function; YGR168C is not an essential gene Gene:YKR073C(YKR073C_d)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR173W-A(YMR173W-A_d)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YNL194C(YNL194C)|FD-Score:-3.8|P-value:7.14E-5||SGD DESC:Integral membrane protein required for sporulation and plasma membrane sphingolipid content; has sequence similarity to SUR7 and FMP45; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; GFP-fusion protein is more abundant at MCCs (membrane compartment occupied by Can1) in the presence of glycerol and oleate Gene:YPL182C(YPL182C_d)|FD-Score:-3.31|P-value:4.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:YPR015C(YPR015C_p)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YRR1(YOR162C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:ZRG8(YER033C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YIL048W10.802.07E-275.64NEO1Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
YER112W5.151.33E-70.63LSM4Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress
YIL062C4.523.13E-60.66ARC15Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity
YGR075C3.855.81E-50.03PRP38Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly
YLR229C3.826.63E-50.00CDC42Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins
YGL207W3.826.77E-50.07SPT16Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome
YMR268C3.749.05E-50.18PRP24Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling
YEL055C3.561.83E-40.20POL5DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA
YIL078W3.363.89E-40.10THS1Threonyl-tRNA synthetase, essential cytoplasmic protein
YKL182W3.275.47E-40.03FAS1Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
YLR230W_d3.246.01E-40.08YLR230W_dDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity
YDR341C3.167.81E-40.15YDR341CArginyl-tRNA synthetase, proposed to be cytoplasmic but the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YNL112W3.020.001280.03DBP2ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
YIR022W2.990.001410.02SEC1118kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum
YDR201W2.970.001490.00SPC19Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR131C11.001.35E-28ACE2Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication
YIR031C6.455.55E-11DAL7Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation
YAL008W6.232.34E-10FUN14Mitochondrial protein of unknown function
YKL212W5.482.08E-8SAC1Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
YPR134W5.161.23E-7MSS18Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions
YHR044C4.671.54E-6DOG12-deoxyglucose-6-phosphate phosphatase; paralogous to DOG2; member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed, in vivo substrate has not yet been identified
YLR219W4.592.21E-6MSC3Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress
YGR052W_p4.592.22E-6FMP48_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation
YJL037W4.483.82E-6IRC18Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci
YBL039W-B_p4.424.96E-6YBL039W-B_pPutative protein of unknown function
YOL155C4.022.92E-5HPF1Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines
YLR405W3.993.25E-5DUS4Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p
YBL028C3.983.41E-5YBL028CProtein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis
YDR492W3.826.63E-5IZH1Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc
YAL031C3.826.68E-5GIP4Cytoplasmic Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate

GO enrichment analysis for SGTC_3316
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2621.84E-93SGTC_1665st014639 11.4 μMTimTec (Natural product derivative library)9811330.0693069NEO1
0.2494.63E-84SGTC_9403232-1287 54.8 μMChemDiv (Drug-like library)15854930.076087NEO1
0.2441.16E-80SGTC_1680diphenhydramine 100.0 μMNIH Clinical Collection89800.121212NEO1
0.2418.31E-79SGTC_1721112-0046 2.6 μMChemDiv (Drug-like library)28021350.102564NEO1
0.2398.69E-78SGTC_270tamoxifen 5.5 μMMiscellaneous53760.105263NEO1
0.2375.21E-76SGTC_2658polymyxin b sulfate 77.0 μMMicrosource (Natural product library)57021050.0692308NEO1
0.2212.29E-66SGTC_8880916-0002 7.0 μMChemDiv (Drug-like library)28021380.102564NEO1
0.2103.78E-60SGTC_1991st073000 8.9 μMTimTec (Natural product derivative library)43019690.175824NEO1
0.2018.80E-55SGTC_5352293-4129 48.2 μMChemDiv (Drug-like library)3961180.117647NEO1
0.1963.12E-52SGTC_1797st048695 56.8 μMTimTec (Natural product derivative library)5922620.0941176NEO1
0.1882.91E-48SGTC_29739094665 18.0 μMChembridge (Drug-like library)252370610.129412NEO1
0.1859.41E-47SGTC_9001112-0045 141.0 μMChemDiv (Drug-like library)562110.138889NEO1
0.1831.05E-45SGTC_1781st038725 66.3 μMTimTec (Natural product derivative library)42702750.0493827NEO1
0.1736.42E-41SGTC_232clozapine 119.0 μMMiscellaneous28180.232877NEO1
0.1701.62E-39SGTC_3133474-0040 5.0 μMChemDiv (Drug-like library)10433750.08NEO1-PIK1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3320913700535.14 μM0.5762714908713Chembridge (Drug-like library)335.4463.39704endomembrane recycling
SGTC_335091504993.57 μM0.5223884910996Chembridge (Drug-like library)355.435643.69214cell wall
SGTC_3257913920632.98 μM0.4516134910041Chembridge (Drug-like library)321.419423.04804
SGTC_334891494643.54 μM0.3943661951442Chembridge (Drug-like library)379.498563.56915
SGTC_3353915164439.28 μM0.3939394909158Chembridge (Drug-like library)309.408722.87614
SGTC_3340914663411.1 μM0.3888894907387Chembridge (Drug-like library)365.471983.14615
SGTC_3351915031221.6 μM0.3768124904905Chembridge (Drug-like library)329.398363.17114cell wall
SGTC_3343914837918.98 μM0.3714291955036Chembridge (Drug-like library)349.472583.96804
SGTC_3333914182012.09 μM0.3623191939433Chembridge (Drug-like library)349.472583.97404
SGTC_3349914970323 μM0.3521134910494Chembridge (Drug-like library)351.44542.50915