9138495

N-(3,4-difluorophenyl)-2-(4-ethyl-2-oxomorpholin-3-yl)acetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3317
Screen concentration 8.8 μM
Source Chembridge (Drug-like library)
PubChem CID 17020139
SMILES CCN1CCOC(=O)C1CC(=O)NC2=CC(=C(C=C2)F)F
Standardized SMILES CCN1CCOC(=O)C1CC(=O)Nc2ccc(F)c(F)c2
Molecular weight 298.2852
ALogP 1.63
H-bond donor count 1
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.96
% growth inhibition (Hom. pool) 5.42


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17020139
Download HIP data (tab-delimited text)  (excel)
Gene:BET3(YKR068C)|FD-Score:3.2|P-value:6.83E-4|Clearance:0.08||SGD DESC:Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex Gene:CAK1(YFL029C)|FD-Score:4.09|P-value:2.20E-5|Clearance:0.03||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CDC16(YKL022C)|FD-Score:-3.2|P-value:6.90E-4|Clearance:0||SGD DESC:Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation Gene:DBP2(YNL112W)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.03||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:GCD7(YLR291C)|FD-Score:3.12|P-value:8.97E-4|Clearance:0.1||SGD DESC:Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GPN3(YLR243W_p)|FD-Score:3.88|P-value:5.31E-5|Clearance:0.14||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p Gene:KRE5(YOR336W)|FD-Score:-4.38|P-value:6.03E-6|Clearance:0||SGD DESC:Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects Gene:LSM5(YER146W)|FD-Score:5.62|P-value:9.63E-9|Clearance:0.44||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MIA40(YKL195W)|FD-Score:8.67|P-value:2.07E-18|Clearance:1.63||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:RBA50(YDR527W)|FD-Score:7.05|P-value:9.12E-13|Clearance:0.97||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RET1(YOR207C)|FD-Score:12.1|P-value:3.45E-34|Clearance:2.34||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RIB5(YBR256C)|FD-Score:-4.45|P-value:4.24E-6|Clearance:0||SGD DESC:Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway Gene:RPC11(YDR045C)|FD-Score:4.05|P-value:2.51E-5|Clearance:0.18||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:RPC53(YDL150W)|FD-Score:6.08|P-value:6.13E-10|Clearance:0.38||SGD DESC:RNA polymerase III subunit C53 Gene:RPN5(YDL147W)|FD-Score:3.66|P-value:1.28E-4|Clearance:0.24||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPN6(YDL097C)|FD-Score:5.7|P-value:6.12E-9|Clearance:0.08||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPP1(YHR062C)|FD-Score:11|P-value:1.63E-28|Clearance:2.34||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT6(YGL048C)|FD-Score:5.18|P-value:1.12E-7|Clearance:1.09||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RTP1(YMR185W_p)|FD-Score:3.33|P-value:4.33E-4|Clearance:0.13||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:SUP35(YDR172W)|FD-Score:3.74|P-value:9.30E-5|Clearance:0.05||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:YNL114C(YNL114C_d)|FD-Score:3.41|P-value:3.24E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:BET3(YKR068C)|FD-Score:3.2|P-value:6.83E-4|Clearance:0.08||SGD DESC:Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex Gene:CAK1(YFL029C)|FD-Score:4.09|P-value:2.20E-5|Clearance:0.03||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CDC16(YKL022C)|FD-Score:-3.2|P-value:6.90E-4|Clearance:0||SGD DESC:Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation Gene:DBP2(YNL112W)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.03||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:GCD7(YLR291C)|FD-Score:3.12|P-value:8.97E-4|Clearance:0.1||SGD DESC:Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GPN3(YLR243W_p)|FD-Score:3.88|P-value:5.31E-5|Clearance:0.14||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p Gene:KRE5(YOR336W)|FD-Score:-4.38|P-value:6.03E-6|Clearance:0||SGD DESC:Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects Gene:LSM5(YER146W)|FD-Score:5.62|P-value:9.63E-9|Clearance:0.44||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MIA40(YKL195W)|FD-Score:8.67|P-value:2.07E-18|Clearance:1.63||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:RBA50(YDR527W)|FD-Score:7.05|P-value:9.12E-13|Clearance:0.97||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RET1(YOR207C)|FD-Score:12.1|P-value:3.45E-34|Clearance:2.34||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RIB5(YBR256C)|FD-Score:-4.45|P-value:4.24E-6|Clearance:0||SGD DESC:Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway Gene:RPC11(YDR045C)|FD-Score:4.05|P-value:2.51E-5|Clearance:0.18||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:RPC53(YDL150W)|FD-Score:6.08|P-value:6.13E-10|Clearance:0.38||SGD DESC:RNA polymerase III subunit C53 Gene:RPN5(YDL147W)|FD-Score:3.66|P-value:1.28E-4|Clearance:0.24||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPN6(YDL097C)|FD-Score:5.7|P-value:6.12E-9|Clearance:0.08||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPP1(YHR062C)|FD-Score:11|P-value:1.63E-28|Clearance:2.34||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT6(YGL048C)|FD-Score:5.18|P-value:1.12E-7|Clearance:1.09||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RTP1(YMR185W_p)|FD-Score:3.33|P-value:4.33E-4|Clearance:0.13||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:SUP35(YDR172W)|FD-Score:3.74|P-value:9.30E-5|Clearance:0.05||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:YNL114C(YNL114C_d)|FD-Score:3.41|P-value:3.24E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17020139
Download HOP data (tab-delimited text)  (excel)
Gene:ACO2(YJL200C)|FD-Score:-3.11|P-value:9.23E-4||SGD DESC:Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol Gene:AFI1(YOR129C)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Arf3p polarization-specific docking factor, required for the polarized distribution of the ADP-ribosylation factor, Arf3p; participates in polarity development and maintenance of a normal haploid budding pattern; interacts with Cnm7p Gene:AIM32(YML050W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ALF1(YNL148C)|FD-Score:5.14|P-value:1.35E-7||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:AYT1(YLL063C)|FD-Score:-3.26|P-value:5.62E-4||SGD DESC:Acetyltransferase; catalyzes trichothecene 3-O-acetylation, suggesting a possible role in trichothecene biosynthesis Gene:BUD27(YFL023W)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CIN1(YOR349W)|FD-Score:7.29|P-value:1.56E-13||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CIN2(YPL241C)|FD-Score:4.72|P-value:1.16E-6||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CLA4(YNL298W)|FD-Score:8.07|P-value:3.58E-16||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CRR1(YLR213C)|FD-Score:-3.25|P-value:5.72E-4||SGD DESC:Putative glycoside hydrolase of the spore wall envelope; required for normal spore wall assembly, possibly for cross-linking between the glucan and chitosan layers; expressed during sporulation Gene:CSG2(YBR036C)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CYS3(YAL012W)|FD-Score:4.58|P-value:2.30E-6||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DAL7(YIR031C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAP1(YPL170W)|FD-Score:4.57|P-value:2.44E-6||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DAS1(YJL149W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C Gene:DST1(YGL043W)|FD-Score:4.53|P-value:2.98E-6||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:GAL11(YOL051W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GET1(YGL020C)|FD-Score:5.75|P-value:4.59E-9||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GET2(YER083C)|FD-Score:10.2|P-value:1.35E-24||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:IES1(YFL013C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IKI3(YLR384C)|FD-Score:3.82|P-value:6.59E-5||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) Gene:KTR4(YBR199W)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family Gene:LTE1(YAL024C)|FD-Score:4.07|P-value:2.38E-5||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MRPL13(YKR006C)|FD-Score:6.82|P-value:4.56E-12||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MTC7(YEL033W_p)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:NGL3(YML118W)|FD-Score:-3.15|P-value:8.17E-4||SGD DESC:3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication Gene:OYE2(YHR179W)|FD-Score:3.11|P-value:9.39E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PAC2(YER007W)|FD-Score:8.54|P-value:6.63E-18||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PBS2(YJL128C)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PDR15(YDR406W)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element Gene:PEP12(YOR036W)|FD-Score:4.27|P-value:9.74E-6||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP3(YLR148W)|FD-Score:4.36|P-value:6.59E-6||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEX29(YDR479C)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PNP1(YLR209C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway Gene:PPH21(YDL134C)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication Gene:RPL24A(YGL031C)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:8.73|P-value:1.27E-18||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SET6(YPL165C)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:SET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability Gene:SHB17(YKR043C)|FD-Score:4.93|P-value:4.17E-7||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SMA2(YML066C)|FD-Score:-3.75|P-value:8.74E-5||SGD DESC:Meiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation Gene:SPO12(YHR152W)|FD-Score:4|P-value:3.21E-5||SGD DESC:Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis Gene:STE23(YLR389C)|FD-Score:-4.14|P-value:1.73E-5||SGD DESC:Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family Gene:STI1(YOR027W)|FD-Score:5.18|P-value:1.14E-7||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:SWC3(YAL011W)|FD-Score:4.81|P-value:7.63E-7||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:SYP1(YCR030C)|FD-Score:-3.29|P-value:5.09E-4||SGD DESC:Protein of unknown function that is involved in endocytic site formation; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization Gene:SYT1(YPR095C)|FD-Score:4.27|P-value:9.98E-6||SGD DESC:Guanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain Gene:TRP1(YDR007W)|FD-Score:6.15|P-value:3.98E-10||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:7.74|P-value:5.01E-15||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:5.82|P-value:2.86E-9||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:7.53|P-value:2.50E-14||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:37.6|P-value:0||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:10.4|P-value:7.42E-26||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UPF3(YGR072W)|FD-Score:-4.06|P-value:2.44E-5||SGD DESC:Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance Gene:UTR1(YJR049C)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VAC17(YCL063W)|FD-Score:3.99|P-value:3.29E-5||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VMA8(YEL051W)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis Gene:VPS71(YML041C)|FD-Score:6.21|P-value:2.69E-10||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VPS72(YDR485C)|FD-Score:5.57|P-value:1.25E-8||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:WSC2(YNL283C)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YBR085C-A(YBR085C-A_p)|FD-Score:-3.18|P-value:7.28E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus; protein abundance increases in response to DNA replication stress Gene:YBR174C(YBR174C_d)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YBR221W-A(YBR221W-A_p)|FD-Score:-4.47|P-value:3.98E-6||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YCR051W(YCR051W_p)|FD-Score:4.59|P-value:2.24E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:6.71|P-value:9.65E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR230W(YDR230W_d)|FD-Score:-3.15|P-value:8.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20 Gene:YEH2(YLR020C)|FD-Score:-3.46|P-value:2.66E-4||SGD DESC:Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism Gene:YHM2(YMR241W)|FD-Score:5.27|P-value:6.92E-8||SGD DESC:Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome Gene:YLR307C-A(YLR307C-A_p)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:Putative protein of unknown function Gene:YOR186W(YOR186W_p)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Putative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication Gene:YOR289W(YOR289W_p)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YOR314W(YOR314W_d)|FD-Score:-3.16|P-value:8.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL062W(YPL062W_d)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPL108W(YPL108W_p)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:ACO2(YJL200C)|FD-Score:-3.11|P-value:9.23E-4||SGD DESC:Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol Gene:AFI1(YOR129C)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Arf3p polarization-specific docking factor, required for the polarized distribution of the ADP-ribosylation factor, Arf3p; participates in polarity development and maintenance of a normal haploid budding pattern; interacts with Cnm7p Gene:AIM32(YML050W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ALF1(YNL148C)|FD-Score:5.14|P-value:1.35E-7||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:AYT1(YLL063C)|FD-Score:-3.26|P-value:5.62E-4||SGD DESC:Acetyltransferase; catalyzes trichothecene 3-O-acetylation, suggesting a possible role in trichothecene biosynthesis Gene:BUD27(YFL023W)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CIN1(YOR349W)|FD-Score:7.29|P-value:1.56E-13||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CIN2(YPL241C)|FD-Score:4.72|P-value:1.16E-6||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CLA4(YNL298W)|FD-Score:8.07|P-value:3.58E-16||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CRR1(YLR213C)|FD-Score:-3.25|P-value:5.72E-4||SGD DESC:Putative glycoside hydrolase of the spore wall envelope; required for normal spore wall assembly, possibly for cross-linking between the glucan and chitosan layers; expressed during sporulation Gene:CSG2(YBR036C)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CYS3(YAL012W)|FD-Score:4.58|P-value:2.30E-6||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DAL7(YIR031C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAP1(YPL170W)|FD-Score:4.57|P-value:2.44E-6||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DAS1(YJL149W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C Gene:DST1(YGL043W)|FD-Score:4.53|P-value:2.98E-6||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:GAL11(YOL051W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GET1(YGL020C)|FD-Score:5.75|P-value:4.59E-9||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GET2(YER083C)|FD-Score:10.2|P-value:1.35E-24||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:IES1(YFL013C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IKI3(YLR384C)|FD-Score:3.82|P-value:6.59E-5||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) Gene:KTR4(YBR199W)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family Gene:LTE1(YAL024C)|FD-Score:4.07|P-value:2.38E-5||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MRPL13(YKR006C)|FD-Score:6.82|P-value:4.56E-12||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MTC7(YEL033W_p)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:NGL3(YML118W)|FD-Score:-3.15|P-value:8.17E-4||SGD DESC:3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication Gene:OYE2(YHR179W)|FD-Score:3.11|P-value:9.39E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PAC2(YER007W)|FD-Score:8.54|P-value:6.63E-18||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PBS2(YJL128C)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PDR15(YDR406W)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element Gene:PEP12(YOR036W)|FD-Score:4.27|P-value:9.74E-6||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP3(YLR148W)|FD-Score:4.36|P-value:6.59E-6||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEX29(YDR479C)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PNP1(YLR209C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway Gene:PPH21(YDL134C)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication Gene:RPL24A(YGL031C)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:8.73|P-value:1.27E-18||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SET6(YPL165C)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:SET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability Gene:SHB17(YKR043C)|FD-Score:4.93|P-value:4.17E-7||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SMA2(YML066C)|FD-Score:-3.75|P-value:8.74E-5||SGD DESC:Meiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation Gene:SPO12(YHR152W)|FD-Score:4|P-value:3.21E-5||SGD DESC:Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis Gene:STE23(YLR389C)|FD-Score:-4.14|P-value:1.73E-5||SGD DESC:Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family Gene:STI1(YOR027W)|FD-Score:5.18|P-value:1.14E-7||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:SWC3(YAL011W)|FD-Score:4.81|P-value:7.63E-7||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:SYP1(YCR030C)|FD-Score:-3.29|P-value:5.09E-4||SGD DESC:Protein of unknown function that is involved in endocytic site formation; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization Gene:SYT1(YPR095C)|FD-Score:4.27|P-value:9.98E-6||SGD DESC:Guanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain Gene:TRP1(YDR007W)|FD-Score:6.15|P-value:3.98E-10||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:7.74|P-value:5.01E-15||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:5.82|P-value:2.86E-9||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:7.53|P-value:2.50E-14||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:37.6|P-value:0||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:10.4|P-value:7.42E-26||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UPF3(YGR072W)|FD-Score:-4.06|P-value:2.44E-5||SGD DESC:Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance Gene:UTR1(YJR049C)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VAC17(YCL063W)|FD-Score:3.99|P-value:3.29E-5||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VMA8(YEL051W)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis Gene:VPS71(YML041C)|FD-Score:6.21|P-value:2.69E-10||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VPS72(YDR485C)|FD-Score:5.57|P-value:1.25E-8||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:WSC2(YNL283C)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YBR085C-A(YBR085C-A_p)|FD-Score:-3.18|P-value:7.28E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus; protein abundance increases in response to DNA replication stress Gene:YBR174C(YBR174C_d)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YBR221W-A(YBR221W-A_p)|FD-Score:-4.47|P-value:3.98E-6||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YCR051W(YCR051W_p)|FD-Score:4.59|P-value:2.24E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:6.71|P-value:9.65E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR230W(YDR230W_d)|FD-Score:-3.15|P-value:8.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20 Gene:YEH2(YLR020C)|FD-Score:-3.46|P-value:2.66E-4||SGD DESC:Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism Gene:YHM2(YMR241W)|FD-Score:5.27|P-value:6.92E-8||SGD DESC:Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome Gene:YLR307C-A(YLR307C-A_p)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:Putative protein of unknown function Gene:YOR186W(YOR186W_p)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Putative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication Gene:YOR289W(YOR289W_p)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YOR314W(YOR314W_d)|FD-Score:-3.16|P-value:8.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL062W(YPL062W_d)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPL108W(YPL108W_p)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS

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Deletion Strain FD score P-value Clearance Gene Gene Description
YOR207C12.103.45E-342.34RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YHR062C11.001.63E-282.34RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YKL195W8.672.07E-181.63MIA40Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
YDR527W7.059.12E-130.97RBA50Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
YDL150W6.086.13E-100.38RPC53RNA polymerase III subunit C53
YDL097C5.706.12E-90.08RPN6Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress
YER146W5.629.63E-90.44LSM5Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
YGL048C5.181.12E-71.09RPT6ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
YFL029C4.092.20E-50.03CAK1Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases
YDR045C4.052.51E-50.18RPC11RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS
YLR243W_p3.885.31E-50.14GPN3_pPutative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p
YDR172W3.749.30E-50.05SUP35Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype
YNL112W3.681.15E-40.03DBP2ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
YDL147W3.661.28E-40.24RPN5Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
YNL114C_d3.413.24E-40.08YNL114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A37.600TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YML124C10.407.42E-26TUB3Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
YER083C10.201.35E-24GET2Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division
YDL020C8.731.27E-18RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YER007W8.546.63E-18PAC2Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
YNL298W8.073.58E-16CLA4Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p
YKL211C7.745.01E-15TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YGL026C7.532.50E-14TRP5Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
YOR349W7.291.56E-13CIN1Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl
YKR006C6.824.56E-12MRPL13Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation
YDR008C_d6.719.65E-12YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YML041C6.212.69E-10VPS71Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
YDR007W6.153.98E-10TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YDR354W5.822.86E-9TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YGL020C5.754.59E-9GET1Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance

GO enrichment analysis for SGTC_3317
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.6100SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.130435
0.5890SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0547945TSC3-RPN4
0.5880SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.112676TSC3-RPN4
0.5770SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.175TSC3-RPN4
0.5750SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.128571TSC3-RPN4
0.5700SGTC_2103551-0445 56.8 μMChemDiv (Drug-like library)28651800.158537
0.5700SGTC_32829108498 15.5 μMChembridge (Drug-like library)164596450.754717
0.5190SGTC_1643st009866 20.2 μMTimTec (Natural product derivative library)31562730.0941176
0.5090SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.0869565TSC3-RPN4
0.5060SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.0684932TSC3-RPN4
0.4880SGTC_23236148992 148.3 μMChembridge (Fragment library)28882200.28125
0.4850SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.123288TSC3-RPN4
0.4780SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.12987TSC3-RPN4
0.4760SGTC_5891227-0070 34.1 μMChemDiv (Drug-like library)28413840.125
0.4581.53E-303SGTC_9262268-1401 59.4 μMChemDiv (Drug-like library)29315780.126582TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_294790726397.68 μM0.82692316488146Chembridge (Drug-like library)314.7398432.08415tubulin folding & SWR complex
SGTC_3282910849815.46 μM0.75471716459645Chembridge (Drug-like library)280.2947831.4215
SGTC_2957907680011.52 μM0.7516459615Chembridge (Drug-like library)290.357482.18614
SGTC_2952907373113.78 μM0.71153816457353Chembridge (Drug-like library)296.749381.87814tubulin folding & SWR complex
SGTC_2962908231113.18 μM0.71153816459453Chembridge (Drug-like library)290.357482.15714
SGTC_296090793895.97 μM0.70909116457355Chembridge (Drug-like library)310.775962.36514
SGTC_2958907725416.72 μM0.68518516457351Chembridge (Drug-like library)292.33031.19815
SGTC_295690761962.52 μM0.67924516423868Chembridge (Drug-like library)331.194442.54314
SGTC_296390825079 μM0.67272716459611Chembridge (Drug-like library)306.356881.54715
SGTC_294990725422.23 μM0.64912316457352Chembridge (Drug-like library)354.399682.77415TSC3-RPN4