9138450

2-methyl-N-(2-morpholin-4-ylethyl)-3-phenyl-5-propan-2-ylpyrazolo[1,5-a]pyrimidin-7-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3319
Screen concentration 16.8 μM
Source Chembridge (Drug-like library)
PubChem CID 2029690
SMILES CC1=NN2C(=CC(=NC2=C1C3=CC=CC=C3)C(C)C)NCCN4CCOCC4
Standardized SMILES CC(C)c1cc(NCCN2CCOCC2)n3nc(C)c(c4ccccc4)c3n1
Molecular weight 379.4986
ALogP 3.49
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 15.91
% growth inhibition (Hom. pool) 9.4


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2029690
Download HIP data (tab-delimited text)  (excel)
Gene:DIM1(YPL266W)|FD-Score:3.32|P-value:4.52E-4|Clearance:0.15||SGD DESC:Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing Gene:FRQ1(YDR373W)|FD-Score:5|P-value:2.86E-7|Clearance:0.67||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:IRA1(YBR140C)|FD-Score:-4.61|P-value:2.00E-6|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LSM3(YLR438C-A)|FD-Score:3.75|P-value:8.86E-5|Clearance:0.16||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:LSM4(YER112W)|FD-Score:4.19|P-value:1.40E-5|Clearance:0.44||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:NUS1(YDL193W)|FD-Score:-3.32|P-value:4.54E-4|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PRP5(YBR237W)|FD-Score:3.17|P-value:7.66E-4|Clearance:0.05||SGD DESC:RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA Gene:RPB10(YOR210W)|FD-Score:-3.15|P-value:8.29E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPC11(YDR045C)|FD-Score:3.46|P-value:2.65E-4|Clearance:0.15||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:RPL32(YBL092W)|FD-Score:-3.8|P-value:7.34E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPS2(YGL123W)|FD-Score:-3.17|P-value:7.73E-4|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:SPN1(YPR133C)|FD-Score:-3.51|P-value:2.27E-4|Clearance:0||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SRP1(YNL189W)|FD-Score:3.48|P-value:2.53E-4|Clearance:0.01||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:SRP14(YDL092W)|FD-Score:-3.17|P-value:7.66E-4|Clearance:0||SGD DESC:Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 Gene:SUI2(YJR007W)|FD-Score:-3.22|P-value:6.34E-4|Clearance:0||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:THS1(YIL078W)|FD-Score:3.59|P-value:1.67E-4|Clearance:0.11||SGD DESC:Threonyl-tRNA synthetase, essential cytoplasmic protein Gene:TOP2(YNL088W)|FD-Score:3.12|P-value:9.04E-4|Clearance:0.11||SGD DESC:Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YDL221W(YDL221W_d)|FD-Score:4.86|P-value:5.78E-7|Clearance:0.67||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13 Gene:DIM1(YPL266W)|FD-Score:3.32|P-value:4.52E-4|Clearance:0.15||SGD DESC:Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing Gene:FRQ1(YDR373W)|FD-Score:5|P-value:2.86E-7|Clearance:0.67||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:IRA1(YBR140C)|FD-Score:-4.61|P-value:2.00E-6|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LSM3(YLR438C-A)|FD-Score:3.75|P-value:8.86E-5|Clearance:0.16||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:LSM4(YER112W)|FD-Score:4.19|P-value:1.40E-5|Clearance:0.44||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:NUS1(YDL193W)|FD-Score:-3.32|P-value:4.54E-4|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PRP5(YBR237W)|FD-Score:3.17|P-value:7.66E-4|Clearance:0.05||SGD DESC:RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA Gene:RPB10(YOR210W)|FD-Score:-3.15|P-value:8.29E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPC11(YDR045C)|FD-Score:3.46|P-value:2.65E-4|Clearance:0.15||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:RPL32(YBL092W)|FD-Score:-3.8|P-value:7.34E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPS2(YGL123W)|FD-Score:-3.17|P-value:7.73E-4|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:SPN1(YPR133C)|FD-Score:-3.51|P-value:2.27E-4|Clearance:0||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SRP1(YNL189W)|FD-Score:3.48|P-value:2.53E-4|Clearance:0.01||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:SRP14(YDL092W)|FD-Score:-3.17|P-value:7.66E-4|Clearance:0||SGD DESC:Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 Gene:SUI2(YJR007W)|FD-Score:-3.22|P-value:6.34E-4|Clearance:0||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:THS1(YIL078W)|FD-Score:3.59|P-value:1.67E-4|Clearance:0.11||SGD DESC:Threonyl-tRNA synthetase, essential cytoplasmic protein Gene:TOP2(YNL088W)|FD-Score:3.12|P-value:9.04E-4|Clearance:0.11||SGD DESC:Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YDL221W(YDL221W_d)|FD-Score:4.86|P-value:5.78E-7|Clearance:0.67||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2029690
Download HOP data (tab-delimited text)  (excel)
Gene:ACA1(YER045C)|FD-Score:3.1|P-value:9.77E-4||SGD DESC:ATF/CREB family basic leucine zipper (bZIP) transcription factor; binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA; important for carbon source utilization; target genes include GRE2 and COS8; ACA1 has a paralog, CST6, that arose from the whole genome duplication Gene:AFG3(YER017C)|FD-Score:-5.14|P-value:1.40E-7||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:ALE1(YOR175C)|FD-Score:-4.5|P-value:3.45E-6||SGD DESC:Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids Gene:APM4(YOL062C)|FD-Score:3.87|P-value:5.47E-5||SGD DESC:Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport Gene:ARC1(YGL105W)|FD-Score:-3.16|P-value:7.98E-4||SGD DESC:Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress Gene:ARO1(YDR127W)|FD-Score:-4.78|P-value:8.81E-7||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG23(YLR431C)|FD-Score:4.61|P-value:2.03E-6||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:BRE1(YDL074C)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:BUD30(YDL151C_d)|FD-Score:4.28|P-value:9.55E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CBC2(YPL178W)|FD-Score:-3.51|P-value:2.20E-4||SGD DESC:Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif Gene:CCM1(YGR150C)|FD-Score:-3.21|P-value:6.53E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CUE5(YOR042W)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DAL81(YIR023W)|FD-Score:-3.97|P-value:3.64E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DAT1(YML113W)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability Gene:DLD2(YDL178W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:EXG2(YDR261C)|FD-Score:3.32|P-value:4.55E-4||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:FLO11(YIR019C)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain Gene:FUM1(YPL262W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GCN3(YKR026C)|FD-Score:4.39|P-value:5.71E-6||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCY1(YOR120W)|FD-Score:4.58|P-value:2.29E-6||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GEP5(YLR091W)|FD-Score:-3.57|P-value:1.75E-4||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:GRX3(YDR098C)|FD-Score:-3.94|P-value:4.11E-5||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; evidence exists indicating that the translation start site is not Met1 as currently annotated, but rather Met36 Gene:HFI1(YPL254W)|FD-Score:-5.33|P-value:4.85E-8||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:ICS3(YJL077C)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 Gene:IDH2(YOR136W)|FD-Score:4.3|P-value:8.41E-6||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IKS1(YJL057C)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Protein kinase of unknown cellular role; putative serine/threonine kinase; expression is induced during mild heat stress; deletion mutants are hypersensitive to copper sulphate and resistant to sorbate; interacts with an N-terminal fragment of Sst2p Gene:KES1(YPL145C)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication Gene:KTR4(YBR199W)|FD-Score:-4.1|P-value:2.07E-5||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family Gene:LST7(YGR057C)|FD-Score:3.98|P-value:3.41E-5||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MAG2(YLR427W)|FD-Score:3.12|P-value:9.10E-4||SGD DESC:Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1 Gene:MEP3(YPR138C)|FD-Score:3.88|P-value:5.27E-5||SGD DESC:Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease Gene:MET5(YJR137C)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine Gene:MNN5(YJL186W)|FD-Score:-3.45|P-value:2.84E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MOS1(YCL057C-A)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MRH1(YDR033W)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Protein that localizes primarily to the plasma membrane; also found at the nuclear envelope; the authentic, non-tagged protein is detected in mitochondria in a phosphorylated state; MRH1 has a paralog, YRO2, that arose from the whole genome duplication Gene:MSW1(YDR268W)|FD-Score:-3.81|P-value:7.00E-5||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MUP1(YGR055W)|FD-Score:4.38|P-value:6.06E-6||SGD DESC:High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake Gene:PET111(YMR257C)|FD-Score:-3.89|P-value:4.95E-5||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PET20(YPL159C)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:PHO88(YBR106W)|FD-Score:-3.94|P-value:4.03E-5||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PUF3(YLL013C)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:PYK2(YOR347C)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication Gene:RAD26(YJR035W)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Protein involved in transcription-coupled nucleotide excision repair of UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein Gene:REX2(YLR059C)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease Gene:RFM1(YOR279C)|FD-Score:4.4|P-value:5.50E-6||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RMD9(YGL107C)|FD-Score:-3.52|P-value:2.13E-4||SGD DESC:Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes Gene:RNR3(YIL066C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Minor isoform of the large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits Gene:RXT2(YBR095C)|FD-Score:-3.29|P-value:5.07E-4||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SAG1(YJR004C)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Alpha-agglutinin of alpha-cells, binds to Aga1p during agglutination, N-terminal half is homologous to the immunoglobulin superfamily and contains binding site for a-agglutinin, C-terminal half is highly glycosylated and contains GPI anchor Gene:SSP120(YLR250W)|FD-Score:-3.54|P-value:1.97E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SUV3(YPL029W)|FD-Score:-3.12|P-value:9.07E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TIP41(YPR040W)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TPA1(YER049W)|FD-Score:3.12|P-value:9.04E-4||SGD DESC:Poly(rA)-binding protein involved in translation termination efficiency, mRNA poly(A) tail length and mRNA stability; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to prolyl 4-hydroxylases; binds Fe(III) and 2-oxoglutarate Gene:VMR1(YHL035C)|FD-Score:4.49|P-value:3.49E-6||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:VPS20(YMR077C)|FD-Score:3.91|P-value:4.61E-5||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:YBR013C(YBR013C_p)|FD-Score:4.36|P-value:6.54E-6||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YCR023C(YCR023C)|FD-Score:6.16|P-value:3.69E-10||SGD DESC:Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene Gene:YCR049C(YCR049C_d)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR095W-A(YCR095W-A_p)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Putative protein of unknown function Gene:YDR169C-A(YDR169C-A_p)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YEL008W(YEL008W_d)|FD-Score:-4.03|P-value:2.85E-5||SGD DESC:Hypothetical protein predicted to be involved in metabolism Gene:YGR283C(YGR283C)|FD-Score:5.61|P-value:1.04E-8||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus Gene:YJL215C(YJL215C_d)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKL107W(YKL107W_p)|FD-Score:4.29|P-value:8.99E-6||SGD DESC:Putative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein Gene:YKL222C(YKL222C)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine Gene:YKR040C(YKR040C_d)|FD-Score:5.49|P-value:2.03E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YKR041W Gene:YME2(YMR302C)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YMR084W(YMR084W_p)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR114C(YMR114C)|FD-Score:5.88|P-value:2.06E-9||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene Gene:YMR172C-A(YMR172C-A_d)|FD-Score:7.37|P-value:8.73E-14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR325W(YOR325W_d)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1 Gene:YPK1(YKL126W)|FD-Score:-3.33|P-value:4.42E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:ACA1(YER045C)|FD-Score:3.1|P-value:9.77E-4||SGD DESC:ATF/CREB family basic leucine zipper (bZIP) transcription factor; binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA; important for carbon source utilization; target genes include GRE2 and COS8; ACA1 has a paralog, CST6, that arose from the whole genome duplication Gene:AFG3(YER017C)|FD-Score:-5.14|P-value:1.40E-7||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:ALE1(YOR175C)|FD-Score:-4.5|P-value:3.45E-6||SGD DESC:Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids Gene:APM4(YOL062C)|FD-Score:3.87|P-value:5.47E-5||SGD DESC:Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport Gene:ARC1(YGL105W)|FD-Score:-3.16|P-value:7.98E-4||SGD DESC:Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress Gene:ARO1(YDR127W)|FD-Score:-4.78|P-value:8.81E-7||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG23(YLR431C)|FD-Score:4.61|P-value:2.03E-6||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:BRE1(YDL074C)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:BUD30(YDL151C_d)|FD-Score:4.28|P-value:9.55E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CBC2(YPL178W)|FD-Score:-3.51|P-value:2.20E-4||SGD DESC:Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif Gene:CCM1(YGR150C)|FD-Score:-3.21|P-value:6.53E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CUE5(YOR042W)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DAL81(YIR023W)|FD-Score:-3.97|P-value:3.64E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DAT1(YML113W)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability Gene:DLD2(YDL178W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:EXG2(YDR261C)|FD-Score:3.32|P-value:4.55E-4||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:FLO11(YIR019C)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain Gene:FUM1(YPL262W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GCN3(YKR026C)|FD-Score:4.39|P-value:5.71E-6||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCY1(YOR120W)|FD-Score:4.58|P-value:2.29E-6||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GEP5(YLR091W)|FD-Score:-3.57|P-value:1.75E-4||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:GRX3(YDR098C)|FD-Score:-3.94|P-value:4.11E-5||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; evidence exists indicating that the translation start site is not Met1 as currently annotated, but rather Met36 Gene:HFI1(YPL254W)|FD-Score:-5.33|P-value:4.85E-8||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:ICS3(YJL077C)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 Gene:IDH2(YOR136W)|FD-Score:4.3|P-value:8.41E-6||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IKS1(YJL057C)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Protein kinase of unknown cellular role; putative serine/threonine kinase; expression is induced during mild heat stress; deletion mutants are hypersensitive to copper sulphate and resistant to sorbate; interacts with an N-terminal fragment of Sst2p Gene:KES1(YPL145C)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication Gene:KTR4(YBR199W)|FD-Score:-4.1|P-value:2.07E-5||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family Gene:LST7(YGR057C)|FD-Score:3.98|P-value:3.41E-5||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MAG2(YLR427W)|FD-Score:3.12|P-value:9.10E-4||SGD DESC:Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1 Gene:MEP3(YPR138C)|FD-Score:3.88|P-value:5.27E-5||SGD DESC:Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease Gene:MET5(YJR137C)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine Gene:MNN5(YJL186W)|FD-Score:-3.45|P-value:2.84E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MOS1(YCL057C-A)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MRH1(YDR033W)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Protein that localizes primarily to the plasma membrane; also found at the nuclear envelope; the authentic, non-tagged protein is detected in mitochondria in a phosphorylated state; MRH1 has a paralog, YRO2, that arose from the whole genome duplication Gene:MSW1(YDR268W)|FD-Score:-3.81|P-value:7.00E-5||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MUP1(YGR055W)|FD-Score:4.38|P-value:6.06E-6||SGD DESC:High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake Gene:PET111(YMR257C)|FD-Score:-3.89|P-value:4.95E-5||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PET20(YPL159C)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:PHO88(YBR106W)|FD-Score:-3.94|P-value:4.03E-5||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PUF3(YLL013C)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:PYK2(YOR347C)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication Gene:RAD26(YJR035W)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Protein involved in transcription-coupled nucleotide excision repair of UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein Gene:REX2(YLR059C)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease Gene:RFM1(YOR279C)|FD-Score:4.4|P-value:5.50E-6||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RMD9(YGL107C)|FD-Score:-3.52|P-value:2.13E-4||SGD DESC:Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes Gene:RNR3(YIL066C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Minor isoform of the large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits Gene:RXT2(YBR095C)|FD-Score:-3.29|P-value:5.07E-4||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SAG1(YJR004C)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Alpha-agglutinin of alpha-cells, binds to Aga1p during agglutination, N-terminal half is homologous to the immunoglobulin superfamily and contains binding site for a-agglutinin, C-terminal half is highly glycosylated and contains GPI anchor Gene:SSP120(YLR250W)|FD-Score:-3.54|P-value:1.97E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SUV3(YPL029W)|FD-Score:-3.12|P-value:9.07E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TIP41(YPR040W)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TPA1(YER049W)|FD-Score:3.12|P-value:9.04E-4||SGD DESC:Poly(rA)-binding protein involved in translation termination efficiency, mRNA poly(A) tail length and mRNA stability; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to prolyl 4-hydroxylases; binds Fe(III) and 2-oxoglutarate Gene:VMR1(YHL035C)|FD-Score:4.49|P-value:3.49E-6||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:VPS20(YMR077C)|FD-Score:3.91|P-value:4.61E-5||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:YBR013C(YBR013C_p)|FD-Score:4.36|P-value:6.54E-6||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YCR023C(YCR023C)|FD-Score:6.16|P-value:3.69E-10||SGD DESC:Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene Gene:YCR049C(YCR049C_d)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR095W-A(YCR095W-A_p)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Putative protein of unknown function Gene:YDR169C-A(YDR169C-A_p)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YEL008W(YEL008W_d)|FD-Score:-4.03|P-value:2.85E-5||SGD DESC:Hypothetical protein predicted to be involved in metabolism Gene:YGR283C(YGR283C)|FD-Score:5.61|P-value:1.04E-8||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus Gene:YJL215C(YJL215C_d)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKL107W(YKL107W_p)|FD-Score:4.29|P-value:8.99E-6||SGD DESC:Putative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein Gene:YKL222C(YKL222C)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine Gene:YKR040C(YKR040C_d)|FD-Score:5.49|P-value:2.03E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YKR041W Gene:YME2(YMR302C)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YMR084W(YMR084W_p)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR114C(YMR114C)|FD-Score:5.88|P-value:2.06E-9||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene Gene:YMR172C-A(YMR172C-A_d)|FD-Score:7.37|P-value:8.73E-14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR325W(YOR325W_d)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1 Gene:YPK1(YKL126W)|FD-Score:-3.33|P-value:4.42E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YDR373W5.002.86E-70.67FRQ1N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily
YDL221W_d4.865.78E-70.67YDL221W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13
YER112W4.191.40E-50.44LSM4Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress
YLR438C-A3.758.86E-50.16LSM3Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YIL078W3.591.67E-40.11THS1Threonyl-tRNA synthetase, essential cytoplasmic protein
YNL189W3.482.53E-40.01SRP1Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation
YDR045C3.462.65E-40.15RPC11RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS
YPL266W3.324.52E-40.15DIM1Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing
YBR237W3.177.66E-40.05PRP5RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA
YNL088W3.129.04E-40.11TOP2Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YGR120C3.010.001320.07COG2Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YOR257W2.940.001640.12CDC31Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
YMR047C2.820.002420.02NUP116FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup159p)
YLR397C2.800.002590.03AFG2ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs
YBR265W2.770.002820.05TSC103-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR172C-A_d7.378.73E-14YMR172C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YCR023C6.163.69E-10YCR023CVacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene
YMR114C5.882.06E-9YMR114CProtein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene
YGR283C5.611.04E-8YGR283CPutative S-adenosylmethionine-dependent methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus
YKR040C_d5.492.03E-8YKR040C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YKR041W
YLR431C4.612.03E-6ATG23Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p
YOR120W4.582.29E-6GCY1Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication
YHL035C4.493.49E-6VMR1Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions
YOR279C4.405.50E-6RFM1DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance
YKR026C4.395.71E-6GCN3Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
YGR055W4.386.06E-6MUP1High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake
YBR013C_p4.366.54E-6YBR013C_pPutative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein
YOR136W4.308.41E-6IDH2Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated
YKL107W_p4.298.99E-6YKL107W_pPutative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein
YDL151C_d4.289.55E-6BUD30_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay

GO enrichment analysis for SGTC_3319
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0831.45E-10SGTC_14053909-7894 12.0 μMChemDiv (Drug-like library)71040600.17721560S ribosome export
0.0815.37E-10SGTC_32229128301 49.5 μMChembridge (Drug-like library)49113580.29870160S ribosome export
0.0765.89E-9SGTC_2670beta-carotene 100.0 μMMicrosource (Natural product library)52804890.0481928
0.0758.30E-9SGTC_30329089427 49.5 μMChembridge (Drug-like library)171737070.13333360S ribosome export
0.0682.07E-7SGTC_32499135183 49.5 μMChembridge (Drug-like library)49027790.64615460S ribosome export
0.0672.39E-7SGTC_29379044753 11.3 μMChembridge (Drug-like library)64655300.086021560S ribosome export
0.0621.70E-6SGTC_2774tamoxifen 10.9 μMMiscellaneous27335260.0987654
0.0621.72E-6SGTC_14063909-7906 11.9 μMChemDiv (Drug-like library)X14060.1728460S ribosome export
0.0621.81E-6SGTC_14073909-7909 12.3 μMChemDiv (Drug-like library)44117800.19047660S ribosome export
0.0594.82E-6SGTC_12690831-0796 3.0 μMChemDiv (Drug-like library)9277580.10975660S ribosome export
0.0595.88E-6SGTC_22047233102 200.0 μMChembridge (Fragment library)21724800.096385560S ribosome export
0.0587.04E-6SGTC_30959117207 49.5 μMChembridge (Drug-like library)170152290.136364
0.0571.17E-5SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.087378660S ribosome export
0.0571.18E-5SGTC_33169136639 10.5 μMChembridge (Drug-like library)49036650.240964
0.0552.19E-5SGTC_1829berberine 14.4 μMTimTec (Natural product derivative library)23530.075268860S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3249913518349.47 μM0.6461544902779Chembridge (Drug-like library)365.471983.0271560S ribosome export
SGTC_3339914588314.94 μM0.5671642030670Chembridge (Drug-like library)379.498563.43515
SGTC_3333914182012.09 μM0.5230771939433Chembridge (Drug-like library)349.472583.97404
SGTC_3258913847749.47 μM0.4533334970355Chembridge (Drug-like library)367.44482.06116
SGTC_334591484103.56 μM0.436624903596Chembridge (Drug-like library)413.514783.9715
SGTC_3326914006416.38 μM0.4133331868434Chembridge (Drug-like library)379.498563.42815
SGTC_3335914331529.63 μM0.4133334899691Chembridge (Drug-like library)365.471982.62615fatty acid desaturase (OLE1)
SGTC_335691518114.48 μM0.3947371859254Chembridge (Drug-like library)357.451524.0914cell wall
SGTC_3343914837918.98 μM0.3835621955036Chembridge (Drug-like library)349.472583.96804
SGTC_331191311462.88 μM0.3783784900406Chembridge (Drug-like library)343.424944.02914cell wall