4112-0506

7-methyl-5,6,7,8-tetrahydro-3H-[1]benzothiolo[2,3-d]pyrimidine-4-thione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_332
Screen concentration 40.9 μM
Source ChemDiv (Drug-like library)
PubChem CID 2790355
SMILES CC1CCC2=C(C1)SC3=C2C(=S)NC=N3
Standardized SMILES CC1CCc2c(C1)sc3ncnc(S)c23
Molecular weight 236.3564
ALogP 3.07
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 55
% growth inhibition (Hom. pool) 16.52


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2790355
Download HIP data (tab-delimited text)  (excel)
Gene:APC4(YDR118W)|FD-Score:3.6|P-value:1.57E-4|Clearance:0.01||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress Gene:ARP4(YJL081C)|FD-Score:5.15|P-value:1.30E-7|Clearance:0.05||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:BET4(YJL031C)|FD-Score:6|P-value:9.85E-10|Clearance:0.05||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:BRL1(YHR036W)|FD-Score:7.58|P-value:1.69E-14|Clearance:0.27||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CDC20(YGL116W)|FD-Score:-3.31|P-value:4.62E-4|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC39(YCR093W)|FD-Score:-4.25|P-value:1.06E-5|Clearance:0||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CKS1(YBR135W)|FD-Score:4.9|P-value:4.78E-7|Clearance:0.21||SGD DESC:Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters Gene:DFR1(YOR236W)|FD-Score:7.75|P-value:4.71E-15|Clearance:0.16||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:ERG9(YHR190W)|FD-Score:6.21|P-value:2.70E-10|Clearance:0.21||SGD DESC:Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway Gene:FHL1(YPR104C)|FD-Score:4.59|P-value:2.19E-6|Clearance:0.2||SGD DESC:Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants Gene:GLE1(YDL207W)|FD-Score:7.78|P-value:3.65E-15|Clearance:0.03||SGD DESC:Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export Gene:HEM12(YDR047W)|FD-Score:7.32|P-value:1.27E-13|Clearance:0.56||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HYP2(YEL034W)|FD-Score:4.14|P-value:1.76E-5|Clearance:0.1||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:LSM2(YBL026W)|FD-Score:4.69|P-value:1.34E-6|Clearance:0.1||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MYO2(YOR326W)|FD-Score:-3.45|P-value:2.81E-4|Clearance:0||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle; MYO2 has a paralog, MYO4, that arose from the whole genome duplication Gene:RAP1(YNL216W)|FD-Score:5.75|P-value:4.58E-9|Clearance:0.59||SGD DESC:Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) Gene:RFC3(YNL290W)|FD-Score:-3.81|P-value:6.93E-5|Clearance:0||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RIM2(YBR192W)|FD-Score:3.8|P-value:7.16E-5|Clearance:0.06||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPB3(YIL021W)|FD-Score:3.36|P-value:3.90E-4|Clearance:0.23||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPL32(YBL092W)|FD-Score:8.89|P-value:3.09E-19|Clearance:1.11||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPN5(YDL147W)|FD-Score:3.6|P-value:1.60E-4|Clearance:0.24||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPN7(YPR108W)|FD-Score:4.4|P-value:5.51E-6|Clearance:0.21||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits Gene:RPN9(YDR427W)|FD-Score:3.7|P-value:1.08E-4|Clearance:0.1||SGD DESC:Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress Gene:RPT6(YGL048C)|FD-Score:4.15|P-value:1.68E-5|Clearance:0.01||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RSC58(YLR033W)|FD-Score:4.19|P-value:1.42E-5|Clearance:0.04||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC9(YML127W)|FD-Score:3.12|P-value:9.17E-4|Clearance:0.02||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SFH1(YLR321C)|FD-Score:3.13|P-value:8.65E-4|Clearance:0.02||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SMD3(YLR147C)|FD-Score:-3.66|P-value:1.26E-4|Clearance:0||SGD DESC:Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 Gene:SPC105(YGL093W)|FD-Score:6.76|P-value:6.91E-12|Clearance:0.5||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SPC98(YNL126W)|FD-Score:3.09|P-value:9.91E-4|Clearance:0.02||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SRP72(YPL210C)|FD-Score:4.04|P-value:2.71E-5|Clearance:0.15||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:STH1(YIL126W)|FD-Score:3.89|P-value:5.10E-5|Clearance:0.06||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:SUP35(YDR172W)|FD-Score:5.95|P-value:1.30E-9|Clearance:0.21||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:SUP45(YBR143C)|FD-Score:3.82|P-value:6.58E-5|Clearance:0.02||SGD DESC:Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p Gene:TIF34(YMR146C)|FD-Score:-3.22|P-value:6.44E-4|Clearance:0||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:TIM12(YBR091C)|FD-Score:6.26|P-value:1.92E-10|Clearance:0.05||SGD DESC:Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins Gene:TIM54(YJL054W)|FD-Score:3.75|P-value:8.95E-5|Clearance:0.05||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:UTR5(YEL035C_p)|FD-Score:5.1|P-value:1.67E-7|Clearance:0.2||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:YTH1(YPR107C)|FD-Score:12.1|P-value:7.62E-34|Clearance:3.18||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:APC4(YDR118W)|FD-Score:3.6|P-value:1.57E-4|Clearance:0.01||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress Gene:ARP4(YJL081C)|FD-Score:5.15|P-value:1.30E-7|Clearance:0.05||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:BET4(YJL031C)|FD-Score:6|P-value:9.85E-10|Clearance:0.05||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:BRL1(YHR036W)|FD-Score:7.58|P-value:1.69E-14|Clearance:0.27||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CDC20(YGL116W)|FD-Score:-3.31|P-value:4.62E-4|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC39(YCR093W)|FD-Score:-4.25|P-value:1.06E-5|Clearance:0||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CKS1(YBR135W)|FD-Score:4.9|P-value:4.78E-7|Clearance:0.21||SGD DESC:Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters Gene:DFR1(YOR236W)|FD-Score:7.75|P-value:4.71E-15|Clearance:0.16||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:ERG9(YHR190W)|FD-Score:6.21|P-value:2.70E-10|Clearance:0.21||SGD DESC:Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway Gene:FHL1(YPR104C)|FD-Score:4.59|P-value:2.19E-6|Clearance:0.2||SGD DESC:Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants Gene:GLE1(YDL207W)|FD-Score:7.78|P-value:3.65E-15|Clearance:0.03||SGD DESC:Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export Gene:HEM12(YDR047W)|FD-Score:7.32|P-value:1.27E-13|Clearance:0.56||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HYP2(YEL034W)|FD-Score:4.14|P-value:1.76E-5|Clearance:0.1||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:LSM2(YBL026W)|FD-Score:4.69|P-value:1.34E-6|Clearance:0.1||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MYO2(YOR326W)|FD-Score:-3.45|P-value:2.81E-4|Clearance:0||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle; MYO2 has a paralog, MYO4, that arose from the whole genome duplication Gene:RAP1(YNL216W)|FD-Score:5.75|P-value:4.58E-9|Clearance:0.59||SGD DESC:Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) Gene:RFC3(YNL290W)|FD-Score:-3.81|P-value:6.93E-5|Clearance:0||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RIM2(YBR192W)|FD-Score:3.8|P-value:7.16E-5|Clearance:0.06||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPB3(YIL021W)|FD-Score:3.36|P-value:3.90E-4|Clearance:0.23||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPL32(YBL092W)|FD-Score:8.89|P-value:3.09E-19|Clearance:1.11||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPN5(YDL147W)|FD-Score:3.6|P-value:1.60E-4|Clearance:0.24||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPN7(YPR108W)|FD-Score:4.4|P-value:5.51E-6|Clearance:0.21||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits Gene:RPN9(YDR427W)|FD-Score:3.7|P-value:1.08E-4|Clearance:0.1||SGD DESC:Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress Gene:RPT6(YGL048C)|FD-Score:4.15|P-value:1.68E-5|Clearance:0.01||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RSC58(YLR033W)|FD-Score:4.19|P-value:1.42E-5|Clearance:0.04||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC9(YML127W)|FD-Score:3.12|P-value:9.17E-4|Clearance:0.02||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SFH1(YLR321C)|FD-Score:3.13|P-value:8.65E-4|Clearance:0.02||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SMD3(YLR147C)|FD-Score:-3.66|P-value:1.26E-4|Clearance:0||SGD DESC:Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 Gene:SPC105(YGL093W)|FD-Score:6.76|P-value:6.91E-12|Clearance:0.5||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SPC98(YNL126W)|FD-Score:3.09|P-value:9.91E-4|Clearance:0.02||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SRP72(YPL210C)|FD-Score:4.04|P-value:2.71E-5|Clearance:0.15||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:STH1(YIL126W)|FD-Score:3.89|P-value:5.10E-5|Clearance:0.06||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:SUP35(YDR172W)|FD-Score:5.95|P-value:1.30E-9|Clearance:0.21||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:SUP45(YBR143C)|FD-Score:3.82|P-value:6.58E-5|Clearance:0.02||SGD DESC:Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p Gene:TIF34(YMR146C)|FD-Score:-3.22|P-value:6.44E-4|Clearance:0||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:TIM12(YBR091C)|FD-Score:6.26|P-value:1.92E-10|Clearance:0.05||SGD DESC:Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins Gene:TIM54(YJL054W)|FD-Score:3.75|P-value:8.95E-5|Clearance:0.05||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:UTR5(YEL035C_p)|FD-Score:5.1|P-value:1.67E-7|Clearance:0.2||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:YTH1(YPR107C)|FD-Score:12.1|P-value:7.62E-34|Clearance:3.18||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2790355
Download HOP data (tab-delimited text)  (excel)
Gene:APL3(YBL037W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport Gene:ARO1(YDR127W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO80(YDR421W)|FD-Score:-3.37|P-value:3.75E-4||SGD DESC:Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids Gene:ATX1(YNL259C)|FD-Score:-3.32|P-value:4.58E-4||SGD DESC:Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake Gene:BTS1(YPL069C)|FD-Score:4.52|P-value:3.10E-6||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CBF1(YJR060W)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CDC50(YCR094W)|FD-Score:5.81|P-value:3.09E-9||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CHS5(YLR330W)|FD-Score:4.84|P-value:6.36E-7||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CPR5(YDR304C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER Gene:CTR1(YPR124W)|FD-Score:-3.4|P-value:3.41E-4||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CUE1(YMR264W)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DSE1(YER124C)|FD-Score:3.89|P-value:4.93E-5||SGD DESC:Daughter cell-specific protein, may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ECM2(YBR065C)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p Gene:ECM33(YBR078W)|FD-Score:4.74|P-value:1.04E-6||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:FIN1(YDR130C)|FD-Score:-3.48|P-value:2.52E-4||SGD DESC:Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress Gene:GCN2(YDR283C)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GYP1(YOR070C)|FD-Score:3.92|P-value:4.41E-5||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HAA1(YPR008W)|FD-Score:-3.14|P-value:8.40E-4||SGD DESC:Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress Gene:HOM6(YJR139C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:IMA1(YGR287C)|FD-Score:-3.18|P-value:7.24E-4||SGD DESC:Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; specificity for isomaltose, alpha-methylglucoside, and palatinose; member of the IMA isomaltase family Gene:IRC4(YDR540C)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:KSP1(YHR082C)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress Gene:LEE1(YPL054W)|FD-Score:-3.35|P-value:4.08E-4||SGD DESC:Zinc-finger protein of unknown function Gene:MDM1(YML104C)|FD-Score:-3.17|P-value:7.61E-4||SGD DESC:Intermediate filament protein, required for nuclear and mitochondrial transmission to daughter buds; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:MSH3(YCR092C)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:NAP1(YKR048C)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:NUM1(YDR150W)|FD-Score:4.48|P-value:3.80E-6||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:PDR16(YNL231C)|FD-Score:5.31|P-value:5.55E-8||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PEP7(YDR323C)|FD-Score:4.54|P-value:2.86E-6||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PHO88(YBR106W)|FD-Score:7.29|P-value:1.54E-13||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:RCY1(YJL204C)|FD-Score:8.42|P-value:1.82E-17||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RGA1(YOR127W)|FD-Score:-3.57|P-value:1.79E-4||SGD DESC:GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RGL1(YPL066W)|FD-Score:5.17|P-value:1.20E-7||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RHO2(YNL090W)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly Gene:RIM8(YGL045W)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:ROM2(YLR371W)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPN4(YDL020C)|FD-Score:9.65|P-value:2.52E-22||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTC4(YNL254C)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:SIC1(YLR079W)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SIS2(YKR072C)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SLG1(YOR008C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SLT2(YHR030C)|FD-Score:4.5|P-value:3.39E-6||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF8(YPL002C)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SNX4(YJL036W)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SOH1(YGL127C)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SPF1(YEL031W)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SSD1(YDR293C)|FD-Score:4.34|P-value:7.27E-6||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SWR1(YDR334W)|FD-Score:3.75|P-value:8.75E-5||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TRM7(YBR061C)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:TRP1(YDR007W)|FD-Score:3.72|P-value:9.86E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP5(YGL026C)|FD-Score:5.57|P-value:1.24E-8||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:8.72|P-value:1.36E-18||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:3.95|P-value:3.97E-5||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UBC7(YMR022W)|FD-Score:3.88|P-value:5.27E-5||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:VAM6(YDL077C)|FD-Score:5.15|P-value:1.32E-7||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VPS41(YDR080W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:YDR455C(YDR455C_d)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YER134C(YER134C)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene Gene:YGR291C(YGR291C_d)|FD-Score:-3.1|P-value:9.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL032C(YIL032C_d)|FD-Score:-3.64|P-value:1.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR018W(YJR018W_d)|FD-Score:5.27|P-value:6.80E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR063W(YLR063W_p)|FD-Score:-3.3|P-value:4.88E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene Gene:YML020W(YML020W_p)|FD-Score:3.96|P-value:3.76E-5||SGD DESC:Putative protein of unknown function Gene:YML131W(YML131W_p)|FD-Score:-4.39|P-value:5.71E-6||SGD DESC:Protein of unknown function; similar to medium chain dehydrogenase/reductases; expression induced by stresses including osmotic shock, DNA damaging agents, and other chemicals; GFP-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YND1(YER005W)|FD-Score:4.43|P-value:4.60E-6||SGD DESC:Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity Gene:YNL089C(YNL089C_d)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins Gene:YNR042W(YNR042W_d)|FD-Score:4.37|P-value:6.08E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2 Gene:YPK1(YKL126W)|FD-Score:5.62|P-value:9.75E-9||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR123C(YPR123C_d)|FD-Score:-3.77|P-value:8.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:APL3(YBL037W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport Gene:ARO1(YDR127W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO80(YDR421W)|FD-Score:-3.37|P-value:3.75E-4||SGD DESC:Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids Gene:ATX1(YNL259C)|FD-Score:-3.32|P-value:4.58E-4||SGD DESC:Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake Gene:BTS1(YPL069C)|FD-Score:4.52|P-value:3.10E-6||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CBF1(YJR060W)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CDC50(YCR094W)|FD-Score:5.81|P-value:3.09E-9||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CHS5(YLR330W)|FD-Score:4.84|P-value:6.36E-7||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CPR5(YDR304C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER Gene:CTR1(YPR124W)|FD-Score:-3.4|P-value:3.41E-4||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CUE1(YMR264W)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DSE1(YER124C)|FD-Score:3.89|P-value:4.93E-5||SGD DESC:Daughter cell-specific protein, may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ECM2(YBR065C)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p Gene:ECM33(YBR078W)|FD-Score:4.74|P-value:1.04E-6||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:FIN1(YDR130C)|FD-Score:-3.48|P-value:2.52E-4||SGD DESC:Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress Gene:GCN2(YDR283C)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GYP1(YOR070C)|FD-Score:3.92|P-value:4.41E-5||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HAA1(YPR008W)|FD-Score:-3.14|P-value:8.40E-4||SGD DESC:Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress Gene:HOM6(YJR139C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:IMA1(YGR287C)|FD-Score:-3.18|P-value:7.24E-4||SGD DESC:Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; specificity for isomaltose, alpha-methylglucoside, and palatinose; member of the IMA isomaltase family Gene:IRC4(YDR540C)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:KSP1(YHR082C)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress Gene:LEE1(YPL054W)|FD-Score:-3.35|P-value:4.08E-4||SGD DESC:Zinc-finger protein of unknown function Gene:MDM1(YML104C)|FD-Score:-3.17|P-value:7.61E-4||SGD DESC:Intermediate filament protein, required for nuclear and mitochondrial transmission to daughter buds; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:MSH3(YCR092C)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:NAP1(YKR048C)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:NUM1(YDR150W)|FD-Score:4.48|P-value:3.80E-6||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:PDR16(YNL231C)|FD-Score:5.31|P-value:5.55E-8||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PEP7(YDR323C)|FD-Score:4.54|P-value:2.86E-6||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PHO88(YBR106W)|FD-Score:7.29|P-value:1.54E-13||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:RCY1(YJL204C)|FD-Score:8.42|P-value:1.82E-17||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RGA1(YOR127W)|FD-Score:-3.57|P-value:1.79E-4||SGD DESC:GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RGL1(YPL066W)|FD-Score:5.17|P-value:1.20E-7||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RHO2(YNL090W)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly Gene:RIM8(YGL045W)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:ROM2(YLR371W)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPN4(YDL020C)|FD-Score:9.65|P-value:2.52E-22||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTC4(YNL254C)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:SIC1(YLR079W)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SIS2(YKR072C)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SLG1(YOR008C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SLT2(YHR030C)|FD-Score:4.5|P-value:3.39E-6||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF8(YPL002C)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SNX4(YJL036W)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SOH1(YGL127C)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SPF1(YEL031W)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SSD1(YDR293C)|FD-Score:4.34|P-value:7.27E-6||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SWR1(YDR334W)|FD-Score:3.75|P-value:8.75E-5||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TRM7(YBR061C)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:TRP1(YDR007W)|FD-Score:3.72|P-value:9.86E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP5(YGL026C)|FD-Score:5.57|P-value:1.24E-8||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:8.72|P-value:1.36E-18||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:3.95|P-value:3.97E-5||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UBC7(YMR022W)|FD-Score:3.88|P-value:5.27E-5||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:VAM6(YDL077C)|FD-Score:5.15|P-value:1.32E-7||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VPS41(YDR080W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:YDR455C(YDR455C_d)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YER134C(YER134C)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene Gene:YGR291C(YGR291C_d)|FD-Score:-3.1|P-value:9.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL032C(YIL032C_d)|FD-Score:-3.64|P-value:1.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR018W(YJR018W_d)|FD-Score:5.27|P-value:6.80E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR063W(YLR063W_p)|FD-Score:-3.3|P-value:4.88E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene Gene:YML020W(YML020W_p)|FD-Score:3.96|P-value:3.76E-5||SGD DESC:Putative protein of unknown function Gene:YML131W(YML131W_p)|FD-Score:-4.39|P-value:5.71E-6||SGD DESC:Protein of unknown function; similar to medium chain dehydrogenase/reductases; expression induced by stresses including osmotic shock, DNA damaging agents, and other chemicals; GFP-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YND1(YER005W)|FD-Score:4.43|P-value:4.60E-6||SGD DESC:Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity Gene:YNL089C(YNL089C_d)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins Gene:YNR042W(YNR042W_d)|FD-Score:4.37|P-value:6.08E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2 Gene:YPK1(YKL126W)|FD-Score:5.62|P-value:9.75E-9||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR123C(YPR123C_d)|FD-Score:-3.77|P-value:8.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR107C12.107.62E-343.18YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YBL092W8.893.09E-191.11RPL32Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog
YDL207W7.783.65E-150.03GLE1Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export
YOR236W7.754.71E-150.16DFR1Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism
YHR036W7.581.69E-140.27BRL1Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
YDR047W7.321.27E-130.56HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YGL093W6.766.91E-120.50SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YBR091C6.261.92E-100.05TIM12Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins
YHR190W6.212.70E-100.21ERG9Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway
YJL031C6.009.85E-100.05BET4Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
YDR172W5.951.30E-90.21SUP35Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype
YNL216W5.754.58E-90.59RAP1Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain)
YJL081C5.151.30E-70.05ARP4Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
YEL035C_p5.101.67E-70.20UTR5_pProtein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions
YBR135W4.904.78E-70.21CKS1Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL020C9.652.52E-22RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YBR058C-A8.721.36E-18TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YJL204C8.421.82E-17RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YBR106W7.291.54E-13PHO88Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations
YCR094W5.813.09E-9CDC50Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
YKL126W5.629.75E-9YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YGL026C5.571.24E-8TRP5Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
YNL231C5.315.55E-8PDR16Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress
YJR018W_d5.276.80E-8YJR018W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YPL066W5.171.20E-7RGL1Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
YDL077C5.151.32E-7VAM6Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p
YLR330W4.846.36E-7CHS5Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane
YBR078W4.741.04E-6ECM33GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p
YDR323C4.542.86E-6PEP7Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
YPL069C4.523.10E-6BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic

GO enrichment analysis for SGTC_332
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3334.87E-152SGTC_5860957-0265 8.2 μMChemDiv (Drug-like library)67523090.0444444cell wall
0.2855.02E-110SGTC_30641327-0027 10.0 μMChemDiv (Drug-like library)160004550.025641
0.2819.47E-108SGTC_5801123-0028 41.0 μMChemDiv (Drug-like library)16772480.0422535DNA damage response
0.2792.53E-106SGTC_1652st011932 78.0 μMTimTec (Natural product derivative library)15510760.0540541RSC complex & mRNA processing
0.2747.40E-102SGTC_14934348-0038 111.0 μMChemDiv (Drug-like library)8004240.0428571RSC complex & mRNA processing
0.2671.03E-96SGTC_21115486399 200.0 μMChembridge (Fragment library)6896740.0625
0.2619.66E-93SGTC_6371181-0519 7.3 μMChemDiv (Drug-like library)62520910.0434783fatty acid desaturase (OLE1)
0.2515.05E-85SGTC_30601327-0027 12.5 μMChemDiv (Drug-like library)160004550.025641
0.2511.49E-85SGTC_8710574-0004 149.0 μMChemDiv (Drug-like library)95511730.0405405
0.2421.17E-79SGTC_13742889-5571 141.0 μMChemDiv (Drug-like library)28532810.0694444RSC complex & mRNA processing
0.2422.52E-79SGTC_1370438-0332 85.4 μMChemDiv (Drug-like library)20633930.0526316ubiquinone biosynthesis & proteasome
0.2424.03E-79SGTC_22256654152 15.4 μMChembridge (Fragment library)975820.0350877
0.2406.62E-78SGTC_21095483026 200.0 μMChembridge (Fragment library)7593350.0862069RSC complex & mRNA processing
0.2372.77E-76SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.03125TSC3-RPN4
0.2331.30E-73SGTC_29127964298 10.9 μMChembridge (Drug-like library)29758500.0416667cell wall

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_8143342-056996 μM0.2878794346283ChemDiv (Drug-like library)305.34893.06125
SGTC_3254913665149.47 μM0.25490216454030Chembridge (Drug-like library)152.193681.43413DNA damage response
SGTC_2139557869256.47 μM0.232143650991Chembridge (Fragment library)207.248962.1904superoxide
SGTC_3384055-0136114 μM0.2121212900563ChemDiv (Drug-like library)286.30581.93515
SGTC_13201313-048195.3 μM0.2105266813365ChemDiv (Drug-like library)355.410881.78216
SGTC_13843089-0840101 μM0.2028991069836ChemDiv (Drug-like library)354.449264.5814
SGTC_597k018-000382.8 μM0.1923086786234ChemDiv (Drug-like library)370.382461.83717RNA pol III & RNase P/MRP
SGTC_2907772198569.9 μM0.1842113267881Chembridge (Drug-like library)326.416024.10814
SGTC_15004488-1866171 μM0.172842900003ChemDiv (Drug-like library)429.575543.71615RPP1 & pyrimidine depletion
SGTC_3142908649849.47 μM0.16666725236367Chembridge (Drug-like library)300.41853.51513iron homeostasis