9138759

1-[4-(3-chlorophenyl)piperazin-1-yl]-3-cyclopentyloxypropan-2-ol hydrochloride

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3324
Screen concentration 60.8 μM
Source Chembridge (Drug-like library)
PubChem CID 45595710
SMILES C1CCC(C1)OCC(CN2CCN(CC2)C3=CC(=CC=C3)Cl)O.Cl
Standardized SMILES OC(COC1CCCC1)CN2CCN(CC2)c3cccc(Cl)c3
Molecular weight 375.3331
ALogP 3.76
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.59
% growth inhibition (Hom. pool) 5.55


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 45595710
Download HIP data (tab-delimited text)  (excel)
Gene:CIA1(YDR267C)|FD-Score:3.2|P-value:6.83E-4|Clearance:0.01||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein Gene:ERG26(YGL001C)|FD-Score:3.12|P-value:8.97E-4|Clearance:0.06||SGD DESC:C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis Gene:KEI1(YDR367W)|FD-Score:5.79|P-value:3.44E-9|Clearance:1.17||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:MEX67(YPL169C)|FD-Score:7.22|P-value:2.68E-13|Clearance:1.42||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:MOB1(YIL106W)|FD-Score:-3.2|P-value:6.79E-4|Clearance:0||SGD DESC:Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:MTR3(YGR158C)|FD-Score:4.35|P-value:6.81E-6|Clearance:0.68||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NBP1(YLR457C)|FD-Score:3.67|P-value:1.19E-4|Clearance:0.11||SGD DESC:Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication Gene:NOG2(YNR053C)|FD-Score:-3.85|P-value:5.86E-5|Clearance:0||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:NSE3(YDR288W)|FD-Score:-5.74|P-value:4.82E-9|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:NUF2(YOL069W)|FD-Score:-7.18|P-value:3.45E-13|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:PGA2(YNL149C)|FD-Score:3.36|P-value:3.86E-4|Clearance:0.04||SGD DESC:Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect Gene:POL12(YBL035C)|FD-Score:-3.46|P-value:2.72E-4|Clearance:0||SGD DESC:B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation Gene:PRP31(YGR091W)|FD-Score:-8.09|P-value:2.90E-16|Clearance:0||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RET2(YFR051C)|FD-Score:3.33|P-value:4.40E-4|Clearance:0.12||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RIX1(YHR197W)|FD-Score:-3.67|P-value:1.20E-4|Clearance:0||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RPB8(YOR224C)|FD-Score:3.57|P-value:1.81E-4|Clearance:0.2||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPL17A(YKL180W)|FD-Score:3.19|P-value:7.13E-4|Clearance:0.07||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPN5(YDL147W)|FD-Score:4.62|P-value:1.89E-6|Clearance:0.27||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:YLR230W(YLR230W_d)|FD-Score:-3.8|P-value:7.16E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity Gene:CIA1(YDR267C)|FD-Score:3.2|P-value:6.83E-4|Clearance:0.01||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein Gene:ERG26(YGL001C)|FD-Score:3.12|P-value:8.97E-4|Clearance:0.06||SGD DESC:C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis Gene:KEI1(YDR367W)|FD-Score:5.79|P-value:3.44E-9|Clearance:1.17||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:MEX67(YPL169C)|FD-Score:7.22|P-value:2.68E-13|Clearance:1.42||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:MOB1(YIL106W)|FD-Score:-3.2|P-value:6.79E-4|Clearance:0||SGD DESC:Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:MTR3(YGR158C)|FD-Score:4.35|P-value:6.81E-6|Clearance:0.68||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NBP1(YLR457C)|FD-Score:3.67|P-value:1.19E-4|Clearance:0.11||SGD DESC:Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication Gene:NOG2(YNR053C)|FD-Score:-3.85|P-value:5.86E-5|Clearance:0||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:NSE3(YDR288W)|FD-Score:-5.74|P-value:4.82E-9|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:NUF2(YOL069W)|FD-Score:-7.18|P-value:3.45E-13|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:PGA2(YNL149C)|FD-Score:3.36|P-value:3.86E-4|Clearance:0.04||SGD DESC:Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect Gene:POL12(YBL035C)|FD-Score:-3.46|P-value:2.72E-4|Clearance:0||SGD DESC:B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation Gene:PRP31(YGR091W)|FD-Score:-8.09|P-value:2.90E-16|Clearance:0||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RET2(YFR051C)|FD-Score:3.33|P-value:4.40E-4|Clearance:0.12||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RIX1(YHR197W)|FD-Score:-3.67|P-value:1.20E-4|Clearance:0||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RPB8(YOR224C)|FD-Score:3.57|P-value:1.81E-4|Clearance:0.2||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPL17A(YKL180W)|FD-Score:3.19|P-value:7.13E-4|Clearance:0.07||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPN5(YDL147W)|FD-Score:4.62|P-value:1.89E-6|Clearance:0.27||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:YLR230W(YLR230W_d)|FD-Score:-3.8|P-value:7.16E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 45595710
Download HOP data (tab-delimited text)  (excel)
Gene:ACN9(YDR511W)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Protein of the mitochondrial intermembrane space, required for acetate utilization and gluconeogenesis; has orthologs in higher eukaryotes Gene:ADH4(YGL256W)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:ALG6(YOR002W)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:API2(YDR525W_d)|FD-Score:4.3|P-value:8.57E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:APL2(YKL135C)|FD-Score:-3.97|P-value:3.62E-5||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:ARD1(YHR013C)|FD-Score:-3.22|P-value:6.39E-4||SGD DESC:Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress Gene:ASI1(YMR119W)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:BAP3(YDR046C)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication Gene:BIO4(YNR057C)|FD-Score:4.87|P-value:5.71E-7||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:BUD19(YJL188C_d)|FD-Score:-8.62|P-value:3.30E-18||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CMK2(YOL016C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication Gene:COG8(YML071C)|FD-Score:-4.04|P-value:2.67E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTS1(YLR286C)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p Gene:DAP1(YPL170W)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:ERG2(YMR202W)|FD-Score:3.76|P-value:8.38E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FCY21(YER060W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FPS1(YLL043W)|FD-Score:3.82|P-value:6.56E-5||SGD DESC:Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress Gene:FYV8(YGR196C)|FD-Score:3.15|P-value:8.23E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GCY1(YOR120W)|FD-Score:3.75|P-value:8.90E-5||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GEP4(YHR100C)|FD-Score:-3.29|P-value:5.09E-4||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GET2(YER083C)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GSC2(YGR032W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p) Gene:HBT1(YDL223C)|FD-Score:5.86|P-value:2.30E-9||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HOM3(YER052C)|FD-Score:5.3|P-value:5.90E-8||SGD DESC:Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:KSP1(YHR082C)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress Gene:MAC1(YMR021C)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MCX1(YBR227C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins Gene:MRPL13(YKR006C)|FD-Score:-6.51|P-value:3.73E-11||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MSF1(YPR047W)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:OSH6(YKR003W)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:PAU23(YLR037C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PER1(YCR044C)|FD-Score:6.6|P-value:2.05E-11||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PET117(YER058W)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Protein required for assembly of cytochrome c oxidase Gene:PHO88(YBR106W)|FD-Score:4.94|P-value:3.89E-7||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PTK1(YKL198C)|FD-Score:-4.78|P-value:8.82E-7||SGD DESC:Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein Gene:RIM8(YGL045W)|FD-Score:3.94|P-value:4.12E-5||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RPS22B(YLR367W)|FD-Score:4.44|P-value:4.58E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication Gene:RRT5(YFR032C_p)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation Gene:SCJ1(YMR214W)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SCS2(YER120W)|FD-Score:4.06|P-value:2.49E-5||SGD DESC:Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication Gene:SHE3(YBR130C)|FD-Score:-5.02|P-value:2.59E-7||SGD DESC:Protein that acts as an adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance Gene:SIP3(YNL257C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SLM3(YDL033C)|FD-Score:-4.01|P-value:3.07E-5||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SPO77(YLR341W)|FD-Score:-3.12|P-value:9.12E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:SSN3(YPL042C)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:TAT2(YOL020W)|FD-Score:3.93|P-value:4.33E-5||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TMA23(YMR269W)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:TRP1(YDR007W)|FD-Score:4.51|P-value:3.21E-6||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP4(YDR354W)|FD-Score:4.41|P-value:5.09E-6||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBP16(YPL072W)|FD-Score:4.02|P-value:2.97E-5||SGD DESC:Deubiquitinating enzyme anchored to the outer mitochondrial membrane, probably not important for general mitochondrial functioning, but may perform a more specialized function at mitochondria Gene:UNG1(YML021C)|FD-Score:4.54|P-value:2.85E-6||SGD DESC:Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus Gene:UTR1(YJR049C)|FD-Score:3.11|P-value:9.34E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VAM6(YDL077C)|FD-Score:5.87|P-value:2.23E-9||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VIK1(YPL253C)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p Gene:YCR101C(YCR101C_p)|FD-Score:-3.26|P-value:5.52E-4||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene Gene:YDR306C(YDR306C_p)|FD-Score:5.64|P-value:8.52E-9||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YDR379C-A(YDR379C-A)|FD-Score:3.19|P-value:7.11E-4||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YDR524W-C(YDR524W-C_p)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin Gene:YDR535C(YDR535C_d)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YFL013W-A(YFL013W-A_d)|FD-Score:5.17|P-value:1.18E-7||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching Gene:YFR012W-A(YFR012W-A_p)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Putative protein of unknown function; identified by homology Gene:YGL118C(YGL118C_d)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL188C-A(YGL188C-A_p)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Putative protein of unknown function Gene:YGR201C(YGR201C_p)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Putative protein of unknown function Gene:YJL068C(YJL068C)|FD-Score:-3.94|P-value:4.13E-5||SGD DESC:Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D Gene:YKL118W(YKL118W_d)|FD-Score:-3.19|P-value:7.01E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR241W(YLR241W_p)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Putative protein of unknown function, may be involved in detoxification Gene:YML047W-A(YML047W-A_d)|FD-Score:-3.89|P-value:4.94E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR166C(YMR166C_p)|FD-Score:3.82|P-value:6.70E-5||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YMR316C-B(YMR316C-B_d)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL144C(YNL144C_p)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study Gene:YOL163W(YOL163W_p)|FD-Score:-3.3|P-value:4.88E-4||SGD DESC:Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family Gene:YOP1(YPR028W)|FD-Score:3.11|P-value:9.51E-4||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YPR012W(YPR012W_d)|FD-Score:-3.84|P-value:6.27E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene Gene:ACN9(YDR511W)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Protein of the mitochondrial intermembrane space, required for acetate utilization and gluconeogenesis; has orthologs in higher eukaryotes Gene:ADH4(YGL256W)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:ALG6(YOR002W)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:API2(YDR525W_d)|FD-Score:4.3|P-value:8.57E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:APL2(YKL135C)|FD-Score:-3.97|P-value:3.62E-5||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:ARD1(YHR013C)|FD-Score:-3.22|P-value:6.39E-4||SGD DESC:Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress Gene:ASI1(YMR119W)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:BAP3(YDR046C)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication Gene:BIO4(YNR057C)|FD-Score:4.87|P-value:5.71E-7||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:BUD19(YJL188C_d)|FD-Score:-8.62|P-value:3.30E-18||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CMK2(YOL016C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication Gene:COG8(YML071C)|FD-Score:-4.04|P-value:2.67E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTS1(YLR286C)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p Gene:DAP1(YPL170W)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:ERG2(YMR202W)|FD-Score:3.76|P-value:8.38E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FCY21(YER060W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FPS1(YLL043W)|FD-Score:3.82|P-value:6.56E-5||SGD DESC:Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress Gene:FYV8(YGR196C)|FD-Score:3.15|P-value:8.23E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GCY1(YOR120W)|FD-Score:3.75|P-value:8.90E-5||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GEP4(YHR100C)|FD-Score:-3.29|P-value:5.09E-4||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GET2(YER083C)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GSC2(YGR032W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p) Gene:HBT1(YDL223C)|FD-Score:5.86|P-value:2.30E-9||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HOM3(YER052C)|FD-Score:5.3|P-value:5.90E-8||SGD DESC:Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:KSP1(YHR082C)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress Gene:MAC1(YMR021C)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MCX1(YBR227C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins Gene:MRPL13(YKR006C)|FD-Score:-6.51|P-value:3.73E-11||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MSF1(YPR047W)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:OSH6(YKR003W)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:PAU23(YLR037C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PER1(YCR044C)|FD-Score:6.6|P-value:2.05E-11||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PET117(YER058W)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Protein required for assembly of cytochrome c oxidase Gene:PHO88(YBR106W)|FD-Score:4.94|P-value:3.89E-7||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PTK1(YKL198C)|FD-Score:-4.78|P-value:8.82E-7||SGD DESC:Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein Gene:RIM8(YGL045W)|FD-Score:3.94|P-value:4.12E-5||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RPS22B(YLR367W)|FD-Score:4.44|P-value:4.58E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication Gene:RRT5(YFR032C_p)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation Gene:SCJ1(YMR214W)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SCS2(YER120W)|FD-Score:4.06|P-value:2.49E-5||SGD DESC:Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication Gene:SHE3(YBR130C)|FD-Score:-5.02|P-value:2.59E-7||SGD DESC:Protein that acts as an adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance Gene:SIP3(YNL257C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SLM3(YDL033C)|FD-Score:-4.01|P-value:3.07E-5||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SPO77(YLR341W)|FD-Score:-3.12|P-value:9.12E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:SSN3(YPL042C)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:TAT2(YOL020W)|FD-Score:3.93|P-value:4.33E-5||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TMA23(YMR269W)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:TRP1(YDR007W)|FD-Score:4.51|P-value:3.21E-6||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP4(YDR354W)|FD-Score:4.41|P-value:5.09E-6||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBP16(YPL072W)|FD-Score:4.02|P-value:2.97E-5||SGD DESC:Deubiquitinating enzyme anchored to the outer mitochondrial membrane, probably not important for general mitochondrial functioning, but may perform a more specialized function at mitochondria Gene:UNG1(YML021C)|FD-Score:4.54|P-value:2.85E-6||SGD DESC:Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus Gene:UTR1(YJR049C)|FD-Score:3.11|P-value:9.34E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VAM6(YDL077C)|FD-Score:5.87|P-value:2.23E-9||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VIK1(YPL253C)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p Gene:YCR101C(YCR101C_p)|FD-Score:-3.26|P-value:5.52E-4||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene Gene:YDR306C(YDR306C_p)|FD-Score:5.64|P-value:8.52E-9||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YDR379C-A(YDR379C-A)|FD-Score:3.19|P-value:7.11E-4||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YDR524W-C(YDR524W-C_p)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin Gene:YDR535C(YDR535C_d)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YFL013W-A(YFL013W-A_d)|FD-Score:5.17|P-value:1.18E-7||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching Gene:YFR012W-A(YFR012W-A_p)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Putative protein of unknown function; identified by homology Gene:YGL118C(YGL118C_d)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL188C-A(YGL188C-A_p)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Putative protein of unknown function Gene:YGR201C(YGR201C_p)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Putative protein of unknown function Gene:YJL068C(YJL068C)|FD-Score:-3.94|P-value:4.13E-5||SGD DESC:Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D Gene:YKL118W(YKL118W_d)|FD-Score:-3.19|P-value:7.01E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR241W(YLR241W_p)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Putative protein of unknown function, may be involved in detoxification Gene:YML047W-A(YML047W-A_d)|FD-Score:-3.89|P-value:4.94E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR166C(YMR166C_p)|FD-Score:3.82|P-value:6.70E-5||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YMR316C-B(YMR316C-B_d)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL144C(YNL144C_p)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study Gene:YOL163W(YOL163W_p)|FD-Score:-3.3|P-value:4.88E-4||SGD DESC:Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family Gene:YOP1(YPR028W)|FD-Score:3.11|P-value:9.51E-4||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YPR012W(YPR012W_d)|FD-Score:-3.84|P-value:6.27E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL169C7.222.68E-131.42MEX67Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
YDR367W5.793.44E-91.17KEI1Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
YDL147W4.621.89E-60.27RPN5Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
YGR158C4.356.81E-60.68MTR3Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
YLR457C3.671.19E-40.11NBP1Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication
YOR224C3.571.81E-40.20RPB8RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III
YNL149C3.363.86E-40.04PGA2Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect
YFR051C3.334.40E-40.12RET2Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
YDR267C3.206.83E-40.01CIA1Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein
YKL180W3.197.13E-40.07RPL17ARibosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
YGL001C3.128.97E-40.06ERG26C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis
YGL245W3.070.001080.01GUS1Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress
YJL034W3.050.001130.10KAR2ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p
YDL055C2.950.001580.12PSA1GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure
YJL025W2.830.002330.01RRN7Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YCR044C6.602.05E-11PER1Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1
YDL077C5.872.23E-9VAM6Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p
YDL223C5.862.30E-9HBT1Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis
YDR306C_p5.648.52E-9YDR306C_pF-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain
YER052C5.305.90E-8HOM3Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
YFL013W-A_d5.171.18E-7YFL013W-A_dDubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching
YBR106W4.943.89E-7PHO88Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations
YNR057C4.875.71E-7BIO4Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels
YML021C4.542.85E-6UNG1Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus
YDR007W4.513.21E-6TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YLR367W4.444.58E-6RPS22BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication
YDR354W4.415.09E-6TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YDR525W_d4.308.57E-6API2_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology
YER083C4.191.40E-5GET2Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division
YGL256W4.171.54E-5ADH4Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency

GO enrichment analysis for SGTC_3324
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0961.57E-13SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.0697674
0.0937.20E-13SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.0649351
0.0937.76E-13SGTC_305-(2-thienylidene)-Rhodanine 28.7 μMChemDiv (Drug-like library)12411320.0555556
0.0921.34E-12SGTC_2420aureobasidin a 50.0 nMMiscellaneous99634300.113043
0.0921.51E-12SGTC_1937st074714 47.2 μMTimTec (Natural product derivative library)172509700.111111
0.0841.08E-10SGTC_2051486-1330 71.9 μMChemDiv (Drug-like library)41188340.0853659
0.0822.48E-10SGTC_2232chlorophetanol 41.5 μMMiscellaneous159070.160714TRP & mitochondrial translation
0.0813.97E-10SGTC_1290088-0017 176.6 μMChemDiv (Drug-like library)1824460.0821918
0.0807.18E-10SGTC_23227413319 38.2 μMChembridge (Fragment library)18404000.1ERAD & cell cycle
0.0782.47E-9SGTC_1999st074831 12.5 μMTimTec (Natural product derivative library)171125230.173333plasma membrane duress
0.0772.75E-9SGTC_1941486-1323 56.9 μMChemDiv (Drug-like library)51787540.0843373
0.0773.77E-9SGTC_240417-1663 219.0 μMChemDiv (Drug-like library)43293900.0674157
0.0764.36E-9SGTC_1569carminic acid 40.6 μMTimTec (Pure natural product library)147490.0666667
0.0764.47E-9SGTC_505gw-5074 63.9 μMICCB bioactive library59242080.0649351
0.0766.21E-9SGTC_1519esterone 74.0 μMTimTec (Pure natural product library)6980.103896DNA intercalators

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3341502-102468.5 μM0.3968253296216ChemDiv (Drug-like library)352.469924.07814
SGTC_12830922-012516.4 μM0.3934433360884ChemDiv (Drug-like library)425.951024.84904
SGTC_1673672-005166.21 μM0.3823533237971ChemDiv (Drug-like library)366.49654.56514
SGTC_14813346-200436 μM0.3661972770522ChemDiv (Drug-like library)390.5346033.98115
SGTC_1721112-00462.64 μM0.352802135ChemDiv (Drug-like library)391.334065.85912NEO1
SGTC_8880916-00026.97 μM0.352802138ChemDiv (Drug-like library)377.307485.40312NEO1
SGTC_2722naftopidil47.16 μM0.3484854418Miscellaneous392.490723.96215
SGTC_1504k297-003330 μM0.3287674388117ChemDiv (Drug-like library)413.966764.69123
SGTC_14823346-20498.48 μM0.3239443826804ChemDiv (Drug-like library)390.516363.84115
SGTC_5154092-1064102 μM0.309091717353ChemDiv (Drug-like library)335.27084.77302plasma membrane duress