9140648

2-methylsulfanyl-4-piperazin-1-yl-6,7,8,9-tetrahydro-[1]benzothiolo[3,2-d]pyrimidine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3327
Screen concentration 26.9 μM
Source Chembridge (Drug-like library)
PubChem CID 8029710
SMILES CSC1=NC2=C(C(=N1)N3CCNCC3)SC4=C2CCCC4
Standardized SMILES CSc1nc(N2CCNCC2)c3sc4CCCCc4c3n1
Molecular weight 320.4761
ALogP 3.91
H-bond donor count 1
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.1
% growth inhibition (Hom. pool) 6.62


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 8029710
Download HIP data (tab-delimited text)  (excel)
Gene:ARC40(YBR234C)|FD-Score:4.53|P-value:2.96E-6|Clearance:0.28||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:EBP2(YKL172W)|FD-Score:-3.42|P-value:3.13E-4|Clearance:0||SGD DESC:Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering Gene:GPN3(YLR243W_p)|FD-Score:3.45|P-value:2.81E-4|Clearance:0.07||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p Gene:ILS1(YBL076C)|FD-Score:3.91|P-value:4.62E-5|Clearance:0.03||SGD DESC:Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A Gene:IQG1(YPL242C)|FD-Score:3.27|P-value:5.36E-4|Clearance:0.01||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MED4(YOR174W)|FD-Score:6.84|P-value:3.85E-12|Clearance:1.34||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NOG2(YNR053C)|FD-Score:-4|P-value:3.21E-5|Clearance:0||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:NOP8(YOL144W)|FD-Score:3.64|P-value:1.38E-4|Clearance:0.11||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:OST2(YOR103C)|FD-Score:-3.21|P-value:6.71E-4|Clearance:0||SGD DESC:Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:POL12(YBL035C)|FD-Score:3.2|P-value:6.86E-4|Clearance:0.07||SGD DESC:B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation Gene:POL5(YEL055C)|FD-Score:3.67|P-value:1.23E-4|Clearance:0.01||SGD DESC:DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA Gene:POP8(YBL018C)|FD-Score:-3.47|P-value:2.58E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRP16(YKR086W)|FD-Score:3.36|P-value:3.95E-4|Clearance:0.02||SGD DESC:DEAH-box RNA helicase involved in second catalytic step of splicing; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step Gene:PSA1(YDL055C)|FD-Score:4.15|P-value:1.66E-5|Clearance:0.04||SGD DESC:GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure Gene:PUP2(YGR253C)|FD-Score:-3.38|P-value:3.56E-4|Clearance:0||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:RIB2(YOL066C)|FD-Score:5.3|P-value:5.87E-8|Clearance:0.05||SGD DESC:Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs Gene:RPL28(YGL103W)|FD-Score:-3.24|P-value:5.93E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L28; homologous to mammalian ribosomal protein L27A and bacterial L15; may have peptidyl transferase activity; can mutate to cycloheximide resistance Gene:RPN3(YER021W)|FD-Score:5.18|P-value:1.09E-7|Clearance:0.32||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPN5(YDL147W)|FD-Score:3.53|P-value:2.07E-4|Clearance:0.06||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPN9(YDR427W)|FD-Score:3.85|P-value:6.01E-5|Clearance:0.17||SGD DESC:Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress Gene:RPR2(YIR015W)|FD-Score:-3.6|P-value:1.62E-4|Clearance:0||SGD DESC:Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress Gene:RPT6(YGL048C)|FD-Score:5.24|P-value:7.91E-8|Clearance:0.06||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RSC4(YKR008W)|FD-Score:4.86|P-value:5.89E-7|Clearance:0.33||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:SEC20(YDR498C)|FD-Score:-4.04|P-value:2.66E-5|Clearance:0||SGD DESC:Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p Gene:SLD2(YKL108W)|FD-Score:3.66|P-value:1.26E-4|Clearance:0.02||SGD DESC:Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress Gene:SPC105(YGL093W)|FD-Score:3.34|P-value:4.18E-4|Clearance:0.07||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SRP102(YKL154W)|FD-Score:3.47|P-value:2.59E-4|Clearance:0.02||SGD DESC:Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane Gene:SUB2(YDL084W)|FD-Score:3.86|P-value:5.56E-5|Clearance:0.02||SGD DESC:Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SUP35(YDR172W)|FD-Score:6.64|P-value:1.58E-11|Clearance:1.34||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:SYF1(YDR416W)|FD-Score:4.11|P-value:1.94E-5|Clearance:0.1||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans Gene:TFB2(YPL122C)|FD-Score:3.38|P-value:3.59E-4|Clearance:0.01||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:THS1(YIL078W)|FD-Score:4.02|P-value:2.95E-5|Clearance:0.11||SGD DESC:Threonyl-tRNA synthetase, essential cytoplasmic protein Gene:TIM54(YJL054W)|FD-Score:-3.71|P-value:1.03E-4|Clearance:0||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:TRZ1(YKR079C)|FD-Score:3.88|P-value:5.15E-5|Clearance:0.02||SGD DESC:tRNA 3'-end processing endonuclease tRNase Z; also localized to mitochondria and interacts genetically with Rex2 exonuclease; homolog of the human candidate prostate cancer susceptibility gene ELAC2 Gene:TSC11(YER093C)|FD-Score:4.25|P-value:1.09E-5|Clearance:0.09||SGD DESC:Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain Gene:UTR5(YEL035C_p)|FD-Score:3.13|P-value:8.72E-4|Clearance:0.05||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:YJL086C(YJL086C_d)|FD-Score:3.26|P-value:5.52E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YMR290W-A(YMR290W-A_d)|FD-Score:3.38|P-value:3.68E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YSH1(YLR277C)|FD-Score:3.67|P-value:1.20E-4|Clearance:0.01||SGD DESC:Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication Gene:YTH1(YPR107C)|FD-Score:6.88|P-value:3.03E-12|Clearance:1.34||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ARC40(YBR234C)|FD-Score:4.53|P-value:2.96E-6|Clearance:0.28||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:EBP2(YKL172W)|FD-Score:-3.42|P-value:3.13E-4|Clearance:0||SGD DESC:Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering Gene:GPN3(YLR243W_p)|FD-Score:3.45|P-value:2.81E-4|Clearance:0.07||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p Gene:ILS1(YBL076C)|FD-Score:3.91|P-value:4.62E-5|Clearance:0.03||SGD DESC:Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A Gene:IQG1(YPL242C)|FD-Score:3.27|P-value:5.36E-4|Clearance:0.01||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MED4(YOR174W)|FD-Score:6.84|P-value:3.85E-12|Clearance:1.34||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NOG2(YNR053C)|FD-Score:-4|P-value:3.21E-5|Clearance:0||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:NOP8(YOL144W)|FD-Score:3.64|P-value:1.38E-4|Clearance:0.11||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:OST2(YOR103C)|FD-Score:-3.21|P-value:6.71E-4|Clearance:0||SGD DESC:Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:POL12(YBL035C)|FD-Score:3.2|P-value:6.86E-4|Clearance:0.07||SGD DESC:B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation Gene:POL5(YEL055C)|FD-Score:3.67|P-value:1.23E-4|Clearance:0.01||SGD DESC:DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA Gene:POP8(YBL018C)|FD-Score:-3.47|P-value:2.58E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRP16(YKR086W)|FD-Score:3.36|P-value:3.95E-4|Clearance:0.02||SGD DESC:DEAH-box RNA helicase involved in second catalytic step of splicing; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step Gene:PSA1(YDL055C)|FD-Score:4.15|P-value:1.66E-5|Clearance:0.04||SGD DESC:GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure Gene:PUP2(YGR253C)|FD-Score:-3.38|P-value:3.56E-4|Clearance:0||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:RIB2(YOL066C)|FD-Score:5.3|P-value:5.87E-8|Clearance:0.05||SGD DESC:Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs Gene:RPL28(YGL103W)|FD-Score:-3.24|P-value:5.93E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L28; homologous to mammalian ribosomal protein L27A and bacterial L15; may have peptidyl transferase activity; can mutate to cycloheximide resistance Gene:RPN3(YER021W)|FD-Score:5.18|P-value:1.09E-7|Clearance:0.32||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPN5(YDL147W)|FD-Score:3.53|P-value:2.07E-4|Clearance:0.06||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPN9(YDR427W)|FD-Score:3.85|P-value:6.01E-5|Clearance:0.17||SGD DESC:Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress Gene:RPR2(YIR015W)|FD-Score:-3.6|P-value:1.62E-4|Clearance:0||SGD DESC:Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress Gene:RPT6(YGL048C)|FD-Score:5.24|P-value:7.91E-8|Clearance:0.06||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RSC4(YKR008W)|FD-Score:4.86|P-value:5.89E-7|Clearance:0.33||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:SEC20(YDR498C)|FD-Score:-4.04|P-value:2.66E-5|Clearance:0||SGD DESC:Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p Gene:SLD2(YKL108W)|FD-Score:3.66|P-value:1.26E-4|Clearance:0.02||SGD DESC:Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress Gene:SPC105(YGL093W)|FD-Score:3.34|P-value:4.18E-4|Clearance:0.07||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SRP102(YKL154W)|FD-Score:3.47|P-value:2.59E-4|Clearance:0.02||SGD DESC:Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane Gene:SUB2(YDL084W)|FD-Score:3.86|P-value:5.56E-5|Clearance:0.02||SGD DESC:Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SUP35(YDR172W)|FD-Score:6.64|P-value:1.58E-11|Clearance:1.34||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:SYF1(YDR416W)|FD-Score:4.11|P-value:1.94E-5|Clearance:0.1||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans Gene:TFB2(YPL122C)|FD-Score:3.38|P-value:3.59E-4|Clearance:0.01||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:THS1(YIL078W)|FD-Score:4.02|P-value:2.95E-5|Clearance:0.11||SGD DESC:Threonyl-tRNA synthetase, essential cytoplasmic protein Gene:TIM54(YJL054W)|FD-Score:-3.71|P-value:1.03E-4|Clearance:0||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:TRZ1(YKR079C)|FD-Score:3.88|P-value:5.15E-5|Clearance:0.02||SGD DESC:tRNA 3'-end processing endonuclease tRNase Z; also localized to mitochondria and interacts genetically with Rex2 exonuclease; homolog of the human candidate prostate cancer susceptibility gene ELAC2 Gene:TSC11(YER093C)|FD-Score:4.25|P-value:1.09E-5|Clearance:0.09||SGD DESC:Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain Gene:UTR5(YEL035C_p)|FD-Score:3.13|P-value:8.72E-4|Clearance:0.05||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:YJL086C(YJL086C_d)|FD-Score:3.26|P-value:5.52E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YMR290W-A(YMR290W-A_d)|FD-Score:3.38|P-value:3.68E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YSH1(YLR277C)|FD-Score:3.67|P-value:1.20E-4|Clearance:0.01||SGD DESC:Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication Gene:YTH1(YPR107C)|FD-Score:6.88|P-value:3.03E-12|Clearance:1.34||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 8029710
Download HOP data (tab-delimited text)  (excel)
Gene:ACE2(YLR131C)|FD-Score:6.22|P-value:2.47E-10||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AGP3(YFL055W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Low-affinity amino acid permease, may act to supply the cell with amino acids as nitrogen source in nitrogen-poor conditions; transcription is induced under conditions of sulfur limitation; plays a role in regulating Ty1 transposition Gene:APJ1(YNL077W)|FD-Score:-3.51|P-value:2.28E-4||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ARI1(YGL157W)|FD-Score:3.1|P-value:9.54E-4||SGD DESC:NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily Gene:ARL1(YBR164C)|FD-Score:-3.33|P-value:4.40E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ASP1(YDR321W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Cytosolic L-asparaginase, involved in asparagine catabolism; catalyzes hydrolysis of L-asparagine to aspartic acid and ammonia, has an important role in therapy of acute lymphoblastic leukemia; synthesized constitutively Gene:BUD19(YJL188C_d)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CAT5(YOR125C)|FD-Score:4.38|P-value:5.98E-6||SGD DESC:Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation Gene:COG8(YML071C)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSM2(YIL132C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; required for accurate chromosome segregation during meiosis Gene:ERG2(YMR202W)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ESC8(YOL017W)|FD-Score:3.99|P-value:3.24E-5||SGD DESC:Protein involved in telomeric and mating-type locus silencing; interacts with Sir2p and also interacts with Gal11p, which is a component of the RNA pol II mediator complex; ESC8 has a paralog, IOC3, that arose from the whole genome duplication Gene:HIR1(YBL008W)|FD-Score:-3.18|P-value:7.35E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores Gene:IML3(YBR107C)|FD-Score:6.15|P-value:3.96E-10||SGD DESC:Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 Gene:IRC10(YOL015W)|FD-Score:-3.13|P-value:8.61E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:MDH2(YOL126C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1 Gene:MNN4(YKL201C)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication Gene:MTC5(YDR128W)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress Gene:NEW1(YPL226W)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:OAZ1(YPL052W)|FD-Score:3.93|P-value:4.20E-5||SGD DESC:Regulator of ornithine decarboxylase (Spe1p), antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p Gene:PCL2(YDL127W)|FD-Score:3.9|P-value:4.81E-5||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; localizes to sites of polarized cell growth Gene:PDA1(YER178W)|FD-Score:-5.94|P-value:1.47E-9||SGD DESC:E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose Gene:PET117(YER058W)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Protein required for assembly of cytochrome c oxidase Gene:PET20(YPL159C)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:PEX1(YKL197C)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis Gene:PHO4(YFR034C)|FD-Score:5.03|P-value:2.43E-7||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PHO84(YML123C)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PRM3(YPL192C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Pheromone-regulated protein required for nuclear envelope fusion during karyogamy; localizes to the outer face of the nuclear membrane; interacts with Kar5p at the spindle pole body Gene:RGT1(YKL038W)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:SAF1(YBR280C)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:F-Box protein involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 Gene:SCM4(YGR049W)|FD-Score:-4.41|P-value:5.12E-6||SGD DESC:Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation Gene:SEC66(YBR171W)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SIN3(YOL004W)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity Gene:SPO77(YLR341W)|FD-Score:-3.8|P-value:7.29E-5||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:SYS1(YJL004C)|FD-Score:-3.88|P-value:5.32E-5||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TEX1(YNL253W)|FD-Score:3.24|P-value:6.08E-4||SGD DESC:Protein involved in mRNA export, component of the transcription export (TREX) complex Gene:THP3(YPR045C)|FD-Score:-3.19|P-value:7.10E-4||SGD DESC:Protein that forms a complex with Csn12p that is recruited to transcribed genes and may have a role in transcription elongation; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes Gene:TPP1(YMR156C)|FD-Score:5.83|P-value:2.74E-9||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:VPS61(YDR136C_d)|FD-Score:-3.11|P-value:9.22E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:-4.97|P-value:3.41E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VTH1(YIL173W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Putative membrane glycoprotein with strong similarity to Vth2p and Pep1p/Vps10p, may be involved in vacuolar protein sorting Gene:YEH2(YLR020C)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism Gene:YER156C(YER156C_p)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 Gene:YGL006W-A(YGL006W-A_p)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YGL199C(YGL199C_d)|FD-Score:4.04|P-value:2.72E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W Gene:YGR283C(YGR283C)|FD-Score:-3.91|P-value:4.52E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus Gene:YJL211C(YJL211C_d)|FD-Score:-3.75|P-value:8.69E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2 Gene:YML047W-A(YML047W-A_d)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR082C(YMR082C_d)|FD-Score:-3.22|P-value:6.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR172C-A(YMR172C-A_d)|FD-Score:-4.22|P-value:1.23E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL146C-A(YNL146C-A_p)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Putative protein of unknown function Gene:YNL205C(YNL205C_d)|FD-Score:4.37|P-value:6.31E-6||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT6(YLR262C)|FD-Score:-3.98|P-value:3.42E-5||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:ZEO1(YOL109W)|FD-Score:-3.42|P-value:3.16E-4||SGD DESC:Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria Gene:ACE2(YLR131C)|FD-Score:6.22|P-value:2.47E-10||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AGP3(YFL055W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Low-affinity amino acid permease, may act to supply the cell with amino acids as nitrogen source in nitrogen-poor conditions; transcription is induced under conditions of sulfur limitation; plays a role in regulating Ty1 transposition Gene:APJ1(YNL077W)|FD-Score:-3.51|P-value:2.28E-4||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ARI1(YGL157W)|FD-Score:3.1|P-value:9.54E-4||SGD DESC:NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily Gene:ARL1(YBR164C)|FD-Score:-3.33|P-value:4.40E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ASP1(YDR321W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Cytosolic L-asparaginase, involved in asparagine catabolism; catalyzes hydrolysis of L-asparagine to aspartic acid and ammonia, has an important role in therapy of acute lymphoblastic leukemia; synthesized constitutively Gene:BUD19(YJL188C_d)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CAT5(YOR125C)|FD-Score:4.38|P-value:5.98E-6||SGD DESC:Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation Gene:COG8(YML071C)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSM2(YIL132C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; required for accurate chromosome segregation during meiosis Gene:ERG2(YMR202W)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ESC8(YOL017W)|FD-Score:3.99|P-value:3.24E-5||SGD DESC:Protein involved in telomeric and mating-type locus silencing; interacts with Sir2p and also interacts with Gal11p, which is a component of the RNA pol II mediator complex; ESC8 has a paralog, IOC3, that arose from the whole genome duplication Gene:HIR1(YBL008W)|FD-Score:-3.18|P-value:7.35E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores Gene:IML3(YBR107C)|FD-Score:6.15|P-value:3.96E-10||SGD DESC:Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 Gene:IRC10(YOL015W)|FD-Score:-3.13|P-value:8.61E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:MDH2(YOL126C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1 Gene:MNN4(YKL201C)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication Gene:MTC5(YDR128W)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress Gene:NEW1(YPL226W)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:OAZ1(YPL052W)|FD-Score:3.93|P-value:4.20E-5||SGD DESC:Regulator of ornithine decarboxylase (Spe1p), antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p Gene:PCL2(YDL127W)|FD-Score:3.9|P-value:4.81E-5||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; localizes to sites of polarized cell growth Gene:PDA1(YER178W)|FD-Score:-5.94|P-value:1.47E-9||SGD DESC:E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose Gene:PET117(YER058W)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Protein required for assembly of cytochrome c oxidase Gene:PET20(YPL159C)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:PEX1(YKL197C)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis Gene:PHO4(YFR034C)|FD-Score:5.03|P-value:2.43E-7||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PHO84(YML123C)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PRM3(YPL192C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Pheromone-regulated protein required for nuclear envelope fusion during karyogamy; localizes to the outer face of the nuclear membrane; interacts with Kar5p at the spindle pole body Gene:RGT1(YKL038W)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:SAF1(YBR280C)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:F-Box protein involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 Gene:SCM4(YGR049W)|FD-Score:-4.41|P-value:5.12E-6||SGD DESC:Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation Gene:SEC66(YBR171W)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SIN3(YOL004W)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity Gene:SPO77(YLR341W)|FD-Score:-3.8|P-value:7.29E-5||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:SYS1(YJL004C)|FD-Score:-3.88|P-value:5.32E-5||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TEX1(YNL253W)|FD-Score:3.24|P-value:6.08E-4||SGD DESC:Protein involved in mRNA export, component of the transcription export (TREX) complex Gene:THP3(YPR045C)|FD-Score:-3.19|P-value:7.10E-4||SGD DESC:Protein that forms a complex with Csn12p that is recruited to transcribed genes and may have a role in transcription elongation; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes Gene:TPP1(YMR156C)|FD-Score:5.83|P-value:2.74E-9||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:VPS61(YDR136C_d)|FD-Score:-3.11|P-value:9.22E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:-4.97|P-value:3.41E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VTH1(YIL173W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Putative membrane glycoprotein with strong similarity to Vth2p and Pep1p/Vps10p, may be involved in vacuolar protein sorting Gene:YEH2(YLR020C)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism Gene:YER156C(YER156C_p)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 Gene:YGL006W-A(YGL006W-A_p)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YGL199C(YGL199C_d)|FD-Score:4.04|P-value:2.72E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W Gene:YGR283C(YGR283C)|FD-Score:-3.91|P-value:4.52E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus Gene:YJL211C(YJL211C_d)|FD-Score:-3.75|P-value:8.69E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2 Gene:YML047W-A(YML047W-A_d)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR082C(YMR082C_d)|FD-Score:-3.22|P-value:6.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR172C-A(YMR172C-A_d)|FD-Score:-4.22|P-value:1.23E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL146C-A(YNL146C-A_p)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Putative protein of unknown function Gene:YNL205C(YNL205C_d)|FD-Score:4.37|P-value:6.31E-6||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT6(YLR262C)|FD-Score:-3.98|P-value:3.42E-5||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:ZEO1(YOL109W)|FD-Score:-3.42|P-value:3.16E-4||SGD DESC:Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR107C6.883.03E-121.34YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YOR174W6.843.85E-121.34MED4Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YDR172W6.641.58E-111.34SUP35Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype
YOL066C5.305.87E-80.06RIB2Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs
YGL048C5.247.91E-80.06RPT6ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
YER021W5.181.09E-70.32RPN3Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
YKR008W4.865.89E-70.33RSC4Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p
YBR234C4.532.96E-60.28ARC40Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YER093C4.251.09E-50.09TSC11Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain
YDL055C4.151.66E-50.04PSA1GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure
YDR416W4.111.94E-50.10SYF1Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans
YIL078W4.022.95E-50.11THS1Threonyl-tRNA synthetase, essential cytoplasmic protein
YBL076C3.914.62E-50.03ILS1Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A
YKR079C3.885.15E-50.02TRZ1tRNA 3'-end processing endonuclease tRNase Z; also localized to mitochondria and interacts genetically with Rex2 exonuclease; homolog of the human candidate prostate cancer susceptibility gene ELAC2
YDL084W3.865.56E-50.02SUB2Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR131C6.222.47E-10ACE2Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication
YBR107C6.153.96E-10IML3Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1
YMR156C5.832.74E-9TPP1DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase
YML123C5.191.03E-7PHO84High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity
YFR034C5.032.43E-7PHO4Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability
YOR125C4.385.98E-6CAT5Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation
YNL205C_d4.376.31E-6YNL205C_dDubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
YLR020C4.171.52E-5YEH2Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism
YGL199C_d4.042.72E-5YGL199C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W
YOL017W3.993.24E-5ESC8Protein involved in telomeric and mating-type locus silencing; interacts with Sir2p and also interacts with Gal11p, which is a component of the RNA pol II mediator complex; ESC8 has a paralog, IOC3, that arose from the whole genome duplication
YKL197C3.963.77E-5PEX1AAA-peroxin that heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis
YPL052W3.934.20E-5OAZ1Regulator of ornithine decarboxylase (Spe1p), antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p
YDL127W3.904.81E-5PCL2Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; localizes to sites of polarized cell growth
YIL132C3.681.18E-4CSM2Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; required for accurate chromosome segregation during meiosis
YOL126C3.671.19E-4MDH2Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1

GO enrichment analysis for SGTC_3327
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1382.51E-26SGTC_30641327-0027 10.0 μMChemDiv (Drug-like library)160004550.0120482
0.1223.95E-21SGTC_22567954252 200.0 μMChembridge (Fragment library)29729200.028169mitochondrial processes
0.1157.75E-19SGTC_30591327-0034 3.0 μMChemDiv (Drug-like library)159985800.037037
0.1126.30E-18SGTC_30631327-0034 4.0 μMChemDiv (Drug-like library)159985800.037037
0.1127.70E-18SGTC_30601327-0027 12.5 μMChemDiv (Drug-like library)160004550.0120482
0.1101.78E-17SGTC_24295902844 64.4 μMMiscellaneous19277900.0416667
0.1102.17E-17SGTC_33189139014 38.6 μMChembridge (Drug-like library)166439840.138889
0.1093.61E-17SGTC_21245246320 200.0 μMChembridge (Fragment library)5793420.0294118heme biosynthesis & mitochondrial translocase
0.1071.27E-16SGTC_23749071832 200.0 μMChembridge (Fragment library)173816980.030303endomembrane recycling
0.1041.07E-15SGTC_8510394-0702 453.0 μMChemDiv (Drug-like library)7626460.104478mitochondrial processes
0.1025.24E-15SGTC_1113artemisinin 1.2 μMTimTec (Natural product library)22400.0394737heme biosynthesis & mitochondrial translocase
0.0992.92E-14SGTC_3324112-0506 40.9 μMChemDiv (Drug-like library)27903550.147059
0.0921.25E-12SGTC_5860957-0265 8.2 μMChemDiv (Drug-like library)67523090.0425532cell wall
0.0912.89E-12SGTC_546peri-Naphthalide 147.0 μMChemDiv (Drug-like library)2238240.0307692DNA damage response
0.0896.25E-12SGTC_1968st069590 43.6 μMTimTec (Natural product derivative library)63840660.025974

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_24036032726181.2 μM0.3888892881040Miscellaneous265.39763.80615
SGTC_8720588-0139234 μM0.3389832047100ChemDiv (Drug-like library)289.395862.8405
SGTC_5953909-800373.7 μM0.321429699517ChemDiv (Drug-like library)259.369883.65704
SGTC_1472k841-0093193 μM0.3125X1472ChemDiv (Drug-like library)345.459113.21750
SGTC_2396534295042.78 μM0.251886677Miscellaneous277.40833.96715RSC complex & mRNA processing
SGTC_7093807-1509243 μM0.251886677ChemDiv (Drug-like library)277.40833.96715RSC complex & mRNA processing
SGTC_11333473-024750 μM0.25711311ChemDiv (Drug-like library)308.422343.35625RSC complex & mRNA processing
SGTC_8184072-270753.5 μM0.245614882871ChemDiv (Drug-like library)272.40844.35313
SGTC_23317980721200 μM0.2222222981221Chembridge (Fragment library)275.414022.03106
SGTC_2139557869256.47 μM0.216667650991Chembridge (Fragment library)207.248962.1904superoxide