9140624

2,5-dimethyl-3-(4-methylphenyl)-7-(4-methylpiperazin-1-yl)pyrazolo[1,5-a]pyrimidine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3328
Screen concentration 29.0 μM
Source Chembridge (Drug-like library)
PubChem CID 4904392
SMILES CC1=CC=C(C=C1)C2=C3N=C(C=C(N3N=C2C)N4CCN(CC4)C)C
Standardized SMILES CN1CCN(CC1)c2cc(C)nc3c(c(C)nn23)c4ccc(C)cc4
Molecular weight 335.446
ALogP 3.33
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 21.13
% growth inhibition (Hom. pool) 9.61


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4904392
Download HIP data (tab-delimited text)  (excel)
Gene:CTF13(YMR094W)|FD-Score:3.48|P-value:2.55E-4|Clearance:0.07||SGD DESC:Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis Gene:LSG1(YGL099W)|FD-Score:3.56|P-value:1.84E-4|Clearance:0||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MET4(YNL103W)|FD-Score:10.3|P-value:2.10E-25|Clearance:5.13||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:MRPS18(YNL306W)|FD-Score:3.1|P-value:9.66E-4|Clearance:0.01||SGD DESC:Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins Gene:NUP57(YGR119C)|FD-Score:-3.34|P-value:4.16E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:POP8(YBL018C)|FD-Score:5.22|P-value:8.98E-8|Clearance:1.39||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPA43(YOR340C)|FD-Score:-3.91|P-value:4.53E-5|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:RPL30(YGL030W)|FD-Score:-3.63|P-value:1.40E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:RPP0(YLR340W)|FD-Score:-3.61|P-value:1.52E-4|Clearance:0||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:SEC26(YDR238C)|FD-Score:3.56|P-value:1.85E-4|Clearance:0.03||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SEC59(YMR013C)|FD-Score:3.41|P-value:3.26E-4|Clearance:0.09||SGD DESC:Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation Gene:TYS1(YGR185C)|FD-Score:3.24|P-value:5.99E-4|Clearance:0.14||SGD DESC:Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; protein abundance increases in response to DNA replication stress Gene:YGR115C(YGR115C_d)|FD-Score:3.82|P-value:6.54E-5|Clearance:0.26||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YOR169C(YOR169C_d)|FD-Score:3.53|P-value:2.08E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GLN4/YOR168W Gene:YRA1(YDR381W)|FD-Score:3.32|P-value:4.52E-4|Clearance:0.08||SGD DESC:RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member Gene:CTF13(YMR094W)|FD-Score:3.48|P-value:2.55E-4|Clearance:0.07||SGD DESC:Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis Gene:LSG1(YGL099W)|FD-Score:3.56|P-value:1.84E-4|Clearance:0||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MET4(YNL103W)|FD-Score:10.3|P-value:2.10E-25|Clearance:5.13||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:MRPS18(YNL306W)|FD-Score:3.1|P-value:9.66E-4|Clearance:0.01||SGD DESC:Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins Gene:NUP57(YGR119C)|FD-Score:-3.34|P-value:4.16E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:POP8(YBL018C)|FD-Score:5.22|P-value:8.98E-8|Clearance:1.39||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPA43(YOR340C)|FD-Score:-3.91|P-value:4.53E-5|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:RPL30(YGL030W)|FD-Score:-3.63|P-value:1.40E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:RPP0(YLR340W)|FD-Score:-3.61|P-value:1.52E-4|Clearance:0||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:SEC26(YDR238C)|FD-Score:3.56|P-value:1.85E-4|Clearance:0.03||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SEC59(YMR013C)|FD-Score:3.41|P-value:3.26E-4|Clearance:0.09||SGD DESC:Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation Gene:TYS1(YGR185C)|FD-Score:3.24|P-value:5.99E-4|Clearance:0.14||SGD DESC:Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; protein abundance increases in response to DNA replication stress Gene:YGR115C(YGR115C_d)|FD-Score:3.82|P-value:6.54E-5|Clearance:0.26||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YOR169C(YOR169C_d)|FD-Score:3.53|P-value:2.08E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GLN4/YOR168W Gene:YRA1(YDR381W)|FD-Score:3.32|P-value:4.52E-4|Clearance:0.08||SGD DESC:RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4904392
Download HOP data (tab-delimited text)  (excel)
Gene:ADH4(YGL256W)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:AHP1(YLR109W)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p Gene:BIO4(YNR057C)|FD-Score:3.91|P-value:4.67E-5||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:BNA4(YBL098W)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease Gene:CCS1(YMR038C)|FD-Score:-4.01|P-value:3.06E-5||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CMR3(YPR013C_p)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Putative zinc finger protein; YPR013C is not an essential gene Gene:CSM1(YCR086W)|FD-Score:-4.6|P-value:2.12E-6||SGD DESC:Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:CTT1(YGR088W)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Cytosolic catalase T, has a role in protection from oxidative damage by hydrogen peroxide Gene:DBP1(YPL119C)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress Gene:DCN1(YLR128W)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation Gene:DIT2(YDR402C)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:N-formyltyrosine oxidase, sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s Gene:DNF3(YMR162C)|FD-Score:5.49|P-value:1.98E-8||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; localizes to the trans-Golgi network; likely involved in protein transport; type 4 P-type ATPase Gene:FMP52(YER004W)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FYV1(YDR024W_d)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin Gene:GIS4(YML006C)|FD-Score:3.91|P-value:4.61E-5||SGD DESC:CAAX box containing protein of unknown function, proposed to be involved in the RAS/cAMP signaling pathway Gene:HAT2(YEL056W)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing Gene:HOM6(YJR139C)|FD-Score:5.64|P-value:8.42E-9||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HOP2(YGL033W)|FD-Score:-4.29|P-value:9.02E-6||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:HST2(YPL015C)|FD-Score:-3.42|P-value:3.19E-4||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:KTR7(YIL085C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:MCK1(YNL307C)|FD-Score:3.97|P-value:3.55E-5||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MDM1(YML104C)|FD-Score:3.73|P-value:9.62E-5||SGD DESC:Intermediate filament protein, required for nuclear and mitochondrial transmission to daughter buds; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:MPC54(YOR177C)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MPP6(YNR024W)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MRS2(YOR334W)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Mitochondrial inner membrane Mg(2+) channel, required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns Gene:MRS3(YJL133W)|FD-Score:5.24|P-value:7.88E-8||SGD DESC:Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication Gene:MSF1(YPR047W)|FD-Score:-3.11|P-value:9.22E-4||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:MTC5(YDR128W)|FD-Score:4.19|P-value:1.38E-5||SGD DESC:Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress Gene:NKP2(YLR315W)|FD-Score:-3.76|P-value:8.61E-5||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2 Gene:NMA111(YNL123W)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NRM1(YNR009W)|FD-Score:5.77|P-value:3.93E-9||SGD DESC:Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase Gene:PCL7(YIL050W)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Pho85p cyclin of the Pho80p subfamily, forms a functional kinase complex with Pho85p which phosphorylates Mmr1p and is regulated by Pho81p; involved in glycogen metabolism, expression is cell-cycle regulated Gene:PER1(YCR044C)|FD-Score:-3.28|P-value:5.12E-4||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:POM152(YMR129W)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Glycoprotein subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NUP210 Gene:PYK2(YOR347C)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication Gene:RPL8B(YLL045C)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Ribosomal 60S subunit protein L8B; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication Gene:RSM24(YDR175C)|FD-Score:-3.16|P-value:7.80E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SAF1(YBR280C)|FD-Score:-4.13|P-value:1.79E-5||SGD DESC:F-Box protein involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 Gene:SAN1(YDR143C)|FD-Score:-3.26|P-value:5.49E-4||SGD DESC:Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition Gene:SHB17(YKR043C)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SIN4(YNL236W)|FD-Score:-3.49|P-value:2.37E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SRL2(YLR082C)|FD-Score:4.43|P-value:4.75E-6||SGD DESC:Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation Gene:SSN3(YPL042C)|FD-Score:-4.02|P-value:2.90E-5||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:THO1(YER063W)|FD-Score:4.56|P-value:2.60E-6||SGD DESC:Conserved nuclear RNA-binding protein; specifically binds to transcribed chromatin in a THO- and RNA-dependent manner, genetically interacts with shuttling hnRNP NAB2; overproduction suppresses transcriptional defect caused by hpr1 mutation Gene:TOS2(YGR221C)|FD-Score:6.56|P-value:2.75E-11||SGD DESC:Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p Gene:UBC13(YDR092W)|FD-Score:4.04|P-value:2.69E-5||SGD DESC:Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus Gene:UBP6(YFR010W)|FD-Score:4.49|P-value:3.57E-6||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:VPS35(YJL154C)|FD-Score:4.28|P-value:9.46E-6||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:YBR012C(YBR012C_d)|FD-Score:-3.33|P-value:4.36E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p Gene:YDR186C(YDR186C)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YDR344C(YDR344C_d)|FD-Score:7.41|P-value:6.21E-14||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR379C-A(YDR379C-A)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YGL199C(YGL199C_d)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W Gene:YGR204C-A(YGR204C-A_p)|FD-Score:3.82|P-value:6.56E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YKL063C(YKL063C_p)|FD-Score:3.97|P-value:3.52E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi Gene:YLR241W(YLR241W_p)|FD-Score:6.55|P-value:2.86E-11||SGD DESC:Putative protein of unknown function, may be involved in detoxification Gene:YLR456W(YLR456W_p)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Putative pyridoxal 5'-phosphate synthase; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2; YLR456W has a paralog, YPR172W, that arose from the whole genome duplication Gene:YNL162W-A(YNL162W-A_p)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Putative protein of unknown function; identified by homology Gene:YNL203C(YNL203C_d)|FD-Score:-3.22|P-value:6.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR041C(YOR041C_d)|FD-Score:-3.95|P-value:3.96E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance Gene:YOR263C(YOR263C_d)|FD-Score:-4.24|P-value:1.10E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF DES3/YOR264W Gene:YPL039W(YPL039W_p)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:YPR109W(YPR109W_p)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Predicted membrane protein; dipoid deletion strain has high budding index Gene:YPR146C(YPR146C_d)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR196W(YPR196W_p)|FD-Score:-4.85|P-value:6.05E-7||SGD DESC:Putative maltose-responsive transcription factor Gene:ADH4(YGL256W)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:AHP1(YLR109W)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p Gene:BIO4(YNR057C)|FD-Score:3.91|P-value:4.67E-5||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:BNA4(YBL098W)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease Gene:CCS1(YMR038C)|FD-Score:-4.01|P-value:3.06E-5||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CMR3(YPR013C_p)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Putative zinc finger protein; YPR013C is not an essential gene Gene:CSM1(YCR086W)|FD-Score:-4.6|P-value:2.12E-6||SGD DESC:Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:CTT1(YGR088W)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Cytosolic catalase T, has a role in protection from oxidative damage by hydrogen peroxide Gene:DBP1(YPL119C)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress Gene:DCN1(YLR128W)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation Gene:DIT2(YDR402C)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:N-formyltyrosine oxidase, sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s Gene:DNF3(YMR162C)|FD-Score:5.49|P-value:1.98E-8||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; localizes to the trans-Golgi network; likely involved in protein transport; type 4 P-type ATPase Gene:FMP52(YER004W)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FYV1(YDR024W_d)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin Gene:GIS4(YML006C)|FD-Score:3.91|P-value:4.61E-5||SGD DESC:CAAX box containing protein of unknown function, proposed to be involved in the RAS/cAMP signaling pathway Gene:HAT2(YEL056W)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing Gene:HOM6(YJR139C)|FD-Score:5.64|P-value:8.42E-9||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HOP2(YGL033W)|FD-Score:-4.29|P-value:9.02E-6||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:HST2(YPL015C)|FD-Score:-3.42|P-value:3.19E-4||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:KTR7(YIL085C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:MCK1(YNL307C)|FD-Score:3.97|P-value:3.55E-5||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MDM1(YML104C)|FD-Score:3.73|P-value:9.62E-5||SGD DESC:Intermediate filament protein, required for nuclear and mitochondrial transmission to daughter buds; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:MPC54(YOR177C)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MPP6(YNR024W)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MRS2(YOR334W)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Mitochondrial inner membrane Mg(2+) channel, required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns Gene:MRS3(YJL133W)|FD-Score:5.24|P-value:7.88E-8||SGD DESC:Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication Gene:MSF1(YPR047W)|FD-Score:-3.11|P-value:9.22E-4||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:MTC5(YDR128W)|FD-Score:4.19|P-value:1.38E-5||SGD DESC:Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress Gene:NKP2(YLR315W)|FD-Score:-3.76|P-value:8.61E-5||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2 Gene:NMA111(YNL123W)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NRM1(YNR009W)|FD-Score:5.77|P-value:3.93E-9||SGD DESC:Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase Gene:PCL7(YIL050W)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Pho85p cyclin of the Pho80p subfamily, forms a functional kinase complex with Pho85p which phosphorylates Mmr1p and is regulated by Pho81p; involved in glycogen metabolism, expression is cell-cycle regulated Gene:PER1(YCR044C)|FD-Score:-3.28|P-value:5.12E-4||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:POM152(YMR129W)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Glycoprotein subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NUP210 Gene:PYK2(YOR347C)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication Gene:RPL8B(YLL045C)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Ribosomal 60S subunit protein L8B; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication Gene:RSM24(YDR175C)|FD-Score:-3.16|P-value:7.80E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SAF1(YBR280C)|FD-Score:-4.13|P-value:1.79E-5||SGD DESC:F-Box protein involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 Gene:SAN1(YDR143C)|FD-Score:-3.26|P-value:5.49E-4||SGD DESC:Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition Gene:SHB17(YKR043C)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SIN4(YNL236W)|FD-Score:-3.49|P-value:2.37E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SRL2(YLR082C)|FD-Score:4.43|P-value:4.75E-6||SGD DESC:Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation Gene:SSN3(YPL042C)|FD-Score:-4.02|P-value:2.90E-5||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:THO1(YER063W)|FD-Score:4.56|P-value:2.60E-6||SGD DESC:Conserved nuclear RNA-binding protein; specifically binds to transcribed chromatin in a THO- and RNA-dependent manner, genetically interacts with shuttling hnRNP NAB2; overproduction suppresses transcriptional defect caused by hpr1 mutation Gene:TOS2(YGR221C)|FD-Score:6.56|P-value:2.75E-11||SGD DESC:Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p Gene:UBC13(YDR092W)|FD-Score:4.04|P-value:2.69E-5||SGD DESC:Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus Gene:UBP6(YFR010W)|FD-Score:4.49|P-value:3.57E-6||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:VPS35(YJL154C)|FD-Score:4.28|P-value:9.46E-6||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:YBR012C(YBR012C_d)|FD-Score:-3.33|P-value:4.36E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p Gene:YDR186C(YDR186C)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YDR344C(YDR344C_d)|FD-Score:7.41|P-value:6.21E-14||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR379C-A(YDR379C-A)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YGL199C(YGL199C_d)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W Gene:YGR204C-A(YGR204C-A_p)|FD-Score:3.82|P-value:6.56E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YKL063C(YKL063C_p)|FD-Score:3.97|P-value:3.52E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi Gene:YLR241W(YLR241W_p)|FD-Score:6.55|P-value:2.86E-11||SGD DESC:Putative protein of unknown function, may be involved in detoxification Gene:YLR456W(YLR456W_p)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Putative pyridoxal 5'-phosphate synthase; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2; YLR456W has a paralog, YPR172W, that arose from the whole genome duplication Gene:YNL162W-A(YNL162W-A_p)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Putative protein of unknown function; identified by homology Gene:YNL203C(YNL203C_d)|FD-Score:-3.22|P-value:6.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR041C(YOR041C_d)|FD-Score:-3.95|P-value:3.96E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance Gene:YOR263C(YOR263C_d)|FD-Score:-4.24|P-value:1.10E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF DES3/YOR264W Gene:YPL039W(YPL039W_p)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:YPR109W(YPR109W_p)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Predicted membrane protein; dipoid deletion strain has high budding index Gene:YPR146C(YPR146C_d)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR196W(YPR196W_p)|FD-Score:-4.85|P-value:6.05E-7||SGD DESC:Putative maltose-responsive transcription factor

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL103W10.302.10E-255.13MET4Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p
YBL018C5.228.98E-81.39POP8Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YGR115C_d3.826.54E-50.26YGR115C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C
YGL099W3.561.84E-40.00LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YDR238C3.561.85E-40.03SEC26Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
YOR169C_d3.532.08E-40.05YOR169C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GLN4/YOR168W
YMR094W3.482.55E-40.07CTF13Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis
YMR013C3.413.26E-40.09SEC59Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation
YDR381W3.324.52E-40.08YRA1RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member
YGR185C3.245.99E-40.14TYS1Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; protein abundance increases in response to DNA replication stress
YNL306W3.109.66E-40.01MRPS18Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins
YMR240C3.090.001000.07CUS1Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p
YPL028W3.020.001260.04ERG10Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis
YPL242C2.980.001430.05IQG1Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress
YOR203W_d2.930.001700.05YOR203W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR344C_d7.416.21E-14YDR344C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YGR221C6.562.75E-11TOS2Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p
YLR241W_p6.552.86E-11YLR241W_pPutative protein of unknown function, may be involved in detoxification
YNR009W5.773.93E-9NRM1Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase
YJR139C5.648.42E-9HOM6Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions
YMR162C5.491.98E-8DNF3Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; localizes to the trans-Golgi network; likely involved in protein transport; type 4 P-type ATPase
YJL133W5.247.88E-8MRS3Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication
YER063W4.562.60E-6THO1Conserved nuclear RNA-binding protein; specifically binds to transcribed chromatin in a THO- and RNA-dependent manner, genetically interacts with shuttling hnRNP NAB2; overproduction suppresses transcriptional defect caused by hpr1 mutation
YFR010W4.493.57E-6UBP6Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance
YLR082C4.434.75E-6SRL2Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation
YJL154C4.289.46E-6VPS35Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
YIL050W4.211.26E-5PCL7Pho85p cyclin of the Pho80p subfamily, forms a functional kinase complex with Pho85p which phosphorylates Mmr1p and is regulated by Pho81p; involved in glycogen metabolism, expression is cell-cycle regulated
YPL119C4.201.35E-5DBP1Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress
YDR128W4.191.38E-5MTC5Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress
YMR129W4.151.65E-5POM152Glycoprotein subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NUP210

GO enrichment analysis for SGTC_3328
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1219.03E-21SGTC_33299141233 27.6 μMChembridge (Drug-like library)49091010.453125
0.1023.18E-15SGTC_492tmb-8 126.0 μMICCB bioactive library54940.0533333
0.1015.59E-15SGTC_14914138-0063 26.5 μMChemDiv (Drug-like library)238832270.126761
0.0758.32E-9SGTC_9242046-0044 21.3 μMChemDiv (Drug-like library)36235890.142857
0.0691.37E-7SGTC_33239138564 53.9 μMChembridge (Drug-like library)439123130.0540541
0.0655.85E-7SGTC_690glyoxal 20.3 μMMiscellaneous7860NA
0.0631.10E-6SGTC_20785212016 200.0 μMChembridge (Fragment library)18997080.045454560S ribosome export
0.0631.17E-6SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.16901460S ribosome export
0.0631.32E-6SGTC_2605digitonin 300.0 nMTimTec (Pure natural product library)27350100.016
0.0612.87E-6SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.14473760S ribosome export
0.0612.99E-6SGTC_22067254556 147.0 μMChembridge (Fragment library)43989050.08450760S ribosome export
0.0596.43E-6SGTC_22277312221 64.1 μMChembridge (Fragment library)8871880.056338
0.0571.37E-5SGTC_1829berberine 14.4 μMTimTec (Natural product derivative library)23530.074074160S ribosome export
0.0561.93E-5SGTC_14073909-7909 12.3 μMChemDiv (Drug-like library)44117800.17567660S ribosome export
0.0552.35E-5SGTC_1983454-2873 16.2 μMChemDiv (Drug-like library)7109570.11764760S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3280914096149.47 μM0.6538464901226Chembridge (Drug-like library)355.864483.5090460S ribosome export
SGTC_3257913920632.98 μM0.644910041Chembridge (Drug-like library)321.419423.04804
SGTC_3333914182012.09 μM0.6226421939433Chembridge (Drug-like library)349.472583.97404
SGTC_3222912830149.47 μM0.6111114911358Chembridge (Drug-like library)375.390813.9330760S ribosome export
SGTC_3343914837918.98 μM0.61955036Chembridge (Drug-like library)349.472583.96804
SGTC_3235913260249.47 μM0.5925934912240Chembridge (Drug-like library)352.430163.31605
SGTC_334591484103.56 μM0.4833334903596Chembridge (Drug-like library)413.514783.9715
SGTC_3349914970323 μM0.4666674910494Chembridge (Drug-like library)351.44542.50915
SGTC_3339914588314.94 μM0.4603172030670Chembridge (Drug-like library)379.498563.43515
SGTC_3326914006416.38 μM0.4531251868434Chembridge (Drug-like library)379.498563.42815