9141233

2-[4-[5-methyl-3-phenyl-2-(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-7-yl]piperazin-1-yl]ethanol

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3329
Screen concentration 27.6 μM
Source Chembridge (Drug-like library)
PubChem CID 4909101
SMILES CC1=NC2=C(C(=NN2C(=C1)N3CCN(CC3)CCO)C(F)(F)F)C4=CC=CC=C4
Standardized SMILES Cc1cc(N2CCN(CCO)CC2)n3nc(c(c4ccccc4)c3n1)C(F)(F)F
Molecular weight 405.4168
ALogP 3.39
H-bond donor count 1
H-bond acceptor count 8
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 24.83
% growth inhibition (Hom. pool) 13.08


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4909101
Download HIP data (tab-delimited text)  (excel)
Gene:BET4(YJL031C)|FD-Score:-3.93|P-value:4.21E-5|Clearance:0||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CCT6(YDR188W)|FD-Score:-6.1|P-value:5.43E-10|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CCT7(YJL111W)|FD-Score:-3.39|P-value:3.49E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:IRA1(YBR140C)|FD-Score:-3.7|P-value:1.07E-4|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LSM8(YJR022W)|FD-Score:4.16|P-value:1.57E-5|Clearance:0.19||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) that is part of spliceosomal U6 snRNP and is also implicated in processing of pre-tRNA, pre-snoRNA, and pre-rRNA Gene:MET4(YNL103W)|FD-Score:12.1|P-value:6.29E-34|Clearance:7.92||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:NIP1(YMR309C)|FD-Score:-3.8|P-value:7.24E-5|Clearance:0||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:OST2(YOR103C)|FD-Score:3.78|P-value:7.75E-5|Clearance:0.2||SGD DESC:Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PRP6(YBR055C)|FD-Score:3.11|P-value:9.46E-4|Clearance:0.07||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RKI1(YOR095C)|FD-Score:3.42|P-value:3.10E-4|Clearance:0.32||SGD DESC:Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis Gene:RPB2(YOR151C)|FD-Score:-3.46|P-value:2.68E-4|Clearance:0||SGD DESC:RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit Gene:RPB3(YIL021W)|FD-Score:-3.41|P-value:3.22E-4|Clearance:0||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPB7(YDR404C)|FD-Score:-3.16|P-value:7.96E-4|Clearance:0||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPB8(YOR224C)|FD-Score:-5|P-value:2.84E-7|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC10(YHR143W-A)|FD-Score:-3.52|P-value:2.19E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:RPL30(YGL030W)|FD-Score:-3.66|P-value:1.27E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:RPO26(YPR187W)|FD-Score:-4.41|P-value:5.23E-6|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPS15(YOL040C)|FD-Score:-3.85|P-value:5.82E-5|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:SAS10(YDL153C)|FD-Score:3.58|P-value:1.71E-4|Clearance:0.16||SGD DESC:Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance Gene:SPN1(YPR133C)|FD-Score:-3.27|P-value:5.37E-4|Clearance:0||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:VTI1(YMR197C)|FD-Score:3.97|P-value:3.62E-5|Clearance:0.19||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Gene:YDL152W(YDL152W_d)|FD-Score:-3.19|P-value:7.13E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:BET4(YJL031C)|FD-Score:-3.93|P-value:4.21E-5|Clearance:0||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CCT6(YDR188W)|FD-Score:-6.1|P-value:5.43E-10|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CCT7(YJL111W)|FD-Score:-3.39|P-value:3.49E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:IRA1(YBR140C)|FD-Score:-3.7|P-value:1.07E-4|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LSM8(YJR022W)|FD-Score:4.16|P-value:1.57E-5|Clearance:0.19||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) that is part of spliceosomal U6 snRNP and is also implicated in processing of pre-tRNA, pre-snoRNA, and pre-rRNA Gene:MET4(YNL103W)|FD-Score:12.1|P-value:6.29E-34|Clearance:7.92||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:NIP1(YMR309C)|FD-Score:-3.8|P-value:7.24E-5|Clearance:0||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:OST2(YOR103C)|FD-Score:3.78|P-value:7.75E-5|Clearance:0.2||SGD DESC:Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PRP6(YBR055C)|FD-Score:3.11|P-value:9.46E-4|Clearance:0.07||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RKI1(YOR095C)|FD-Score:3.42|P-value:3.10E-4|Clearance:0.32||SGD DESC:Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis Gene:RPB2(YOR151C)|FD-Score:-3.46|P-value:2.68E-4|Clearance:0||SGD DESC:RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit Gene:RPB3(YIL021W)|FD-Score:-3.41|P-value:3.22E-4|Clearance:0||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPB7(YDR404C)|FD-Score:-3.16|P-value:7.96E-4|Clearance:0||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPB8(YOR224C)|FD-Score:-5|P-value:2.84E-7|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC10(YHR143W-A)|FD-Score:-3.52|P-value:2.19E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:RPL30(YGL030W)|FD-Score:-3.66|P-value:1.27E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:RPO26(YPR187W)|FD-Score:-4.41|P-value:5.23E-6|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPS15(YOL040C)|FD-Score:-3.85|P-value:5.82E-5|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:SAS10(YDL153C)|FD-Score:3.58|P-value:1.71E-4|Clearance:0.16||SGD DESC:Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance Gene:SPN1(YPR133C)|FD-Score:-3.27|P-value:5.37E-4|Clearance:0||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:VTI1(YMR197C)|FD-Score:3.97|P-value:3.62E-5|Clearance:0.19||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Gene:YDL152W(YDL152W_d)|FD-Score:-3.19|P-value:7.13E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4909101
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARG81(YML099C)|FD-Score:7.24|P-value:2.27E-13||SGD DESC:Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p Gene:ATP14(YLR295C)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:BNI1(YNL271C)|FD-Score:-3.92|P-value:4.36E-5||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BUD27(YFL023W)|FD-Score:-3.15|P-value:8.22E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CDC73(YLR418C)|FD-Score:-3.12|P-value:8.94E-4||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CLB6(YGR109C)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 Gene:COX7(YMR256C)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:DYN2(YDR424C)|FD-Score:3.99|P-value:3.35E-5||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:EHT1(YBR177C)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication Gene:END3(YNL084C)|FD-Score:4.62|P-value:1.96E-6||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERV14(YGL054C)|FD-Score:-4.02|P-value:2.90E-5||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:GAS2(YLR343W)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:1,3-beta-glucanosyltransferase, involved with Gas4p in spore wall assembly; has similarity to Gas1p Gene:GAS4(YOL132W)|FD-Score:3.12|P-value:8.98E-4||SGD DESC:1,3-beta-glucanosyltransferase, involved with Gas2p in spore wall assembly; has similarity to Gas1p; localizes to the cell wall Gene:GID7(YCL039W)|FD-Score:5.1|P-value:1.72E-7||SGD DESC:Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions Gene:GIN4(YDR507C)|FD-Score:-4.18|P-value:1.46E-5||SGD DESC:Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication Gene:GRX6(YDL010W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:HIR1(YBL008W)|FD-Score:5.08|P-value:1.92E-7||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores Gene:HSL1(YKL101W)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p Gene:HSP26(YBR072W)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity Gene:IMP2'(YIL154C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:ITR2(YOL103W)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively Gene:LEU4(YNL104C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication Gene:MHT1(YLL062C)|FD-Score:3.77|P-value:8.28E-5||SGD DESC:S-methylmethionine-homocysteine methyltransferase, functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio Gene:MRH4(YGL064C)|FD-Score:-4.99|P-value:3.00E-7||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MRPS8(YMR158W)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MUK1(YPL070W)|FD-Score:5.13|P-value:1.49E-7||SGD DESC:Cytoplasmic protein of unknown function containing a Vps9 domain; computational analysis of large-scale protein-protein interaction data suggests a possible role in transcriptional regulation Gene:NRM1(YNR009W)|FD-Score:5.04|P-value:2.27E-7||SGD DESC:Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase Gene:NRT1(YOR071C)|FD-Score:-3.29|P-value:4.97E-4||SGD DESC:High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity Gene:PAN3(YKL025C)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PAN5(YHR063C)|FD-Score:-3.33|P-value:4.40E-4||SGD DESC:2-dehydropantoate 2-reductase, part of the pantothenic acid pathway, structurally homologous to E. coli panE Gene:PHB1(YGR132C)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation Gene:PHO88(YBR106W)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PTK1(YKL198C)|FD-Score:4.24|P-value:1.09E-5||SGD DESC:Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein Gene:PUS7(YOR243C)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RBG1(YAL036C)|FD-Score:4.67|P-value:1.49E-6||SGD DESC:Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid Gene:RDS1(YCR106W)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:RMD1(YDL001W)|FD-Score:3.75|P-value:8.97E-5||SGD DESC:Cytoplasmic protein required for sporulation Gene:RMD6(YEL072W)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Protein required for sporulation Gene:RPS10A(YOR293W)|FD-Score:-3.42|P-value:3.18E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication Gene:SAM4(YPL273W)|FD-Score:3.81|P-value:6.84E-5||SGD DESC:S-adenosylmethionine-homocysteine methyltransferase, functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio Gene:SAP30(YMR263W)|FD-Score:-3.77|P-value:8.28E-5||SGD DESC:Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance Gene:SDH2(YLL041C)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SGF73(YGL066W)|FD-Score:-3.24|P-value:5.93E-4||SGD DESC:SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SLX8(YER116C)|FD-Score:-3.48|P-value:2.55E-4||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:SPS18(YNL204C)|FD-Score:3.89|P-value:5.09E-5||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SRX1(YKL086W)|FD-Score:4.48|P-value:3.75E-6||SGD DESC:Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress Gene:STE20(YHL007C)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p Gene:SWS2(YNL081C)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TDA9(YML081W)|FD-Score:-3.23|P-value:6.17E-4||SGD DESC:DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication Gene:TIR3(YIL011W)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth Gene:TOK1(YJL093C)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin Gene:YAT1(YAR035W)|FD-Score:5.74|P-value:4.66E-9||SGD DESC:Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated Gene:YBR090C(YBR090C_p)|FD-Score:-3.11|P-value:9.48E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YCR006C(YCR006C_d)|FD-Score:4.08|P-value:2.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER077C(YER077C_p)|FD-Score:-4.15|P-value:1.67E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YGL218W(YGL218W_d)|FD-Score:-3.19|P-value:7.06E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance Gene:YML003W(YML003W_p)|FD-Score:4.93|P-value:4.05E-7||SGD DESC:Putative protein of unknown function Gene:YNL022C(YNL022C_p)|FD-Score:-3.33|P-value:4.39E-4||SGD DESC:Putative cytosine 5-methyltransferase, contains seven beta-strand methyltransferase motif similar to NOP2/YNL061W; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; predicted to be involved in ribosome biogenesis Gene:YOL037C(YOL037C_d)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W Gene:YPL114W(YPL114W_d)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index Gene:AFT1(YGL071W)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARG81(YML099C)|FD-Score:7.24|P-value:2.27E-13||SGD DESC:Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p Gene:ATP14(YLR295C)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:BNI1(YNL271C)|FD-Score:-3.92|P-value:4.36E-5||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BUD27(YFL023W)|FD-Score:-3.15|P-value:8.22E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CDC73(YLR418C)|FD-Score:-3.12|P-value:8.94E-4||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CLB6(YGR109C)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 Gene:COX7(YMR256C)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:DYN2(YDR424C)|FD-Score:3.99|P-value:3.35E-5||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:EHT1(YBR177C)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication Gene:END3(YNL084C)|FD-Score:4.62|P-value:1.96E-6||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERV14(YGL054C)|FD-Score:-4.02|P-value:2.90E-5||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:GAS2(YLR343W)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:1,3-beta-glucanosyltransferase, involved with Gas4p in spore wall assembly; has similarity to Gas1p Gene:GAS4(YOL132W)|FD-Score:3.12|P-value:8.98E-4||SGD DESC:1,3-beta-glucanosyltransferase, involved with Gas2p in spore wall assembly; has similarity to Gas1p; localizes to the cell wall Gene:GID7(YCL039W)|FD-Score:5.1|P-value:1.72E-7||SGD DESC:Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions Gene:GIN4(YDR507C)|FD-Score:-4.18|P-value:1.46E-5||SGD DESC:Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication Gene:GRX6(YDL010W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:HIR1(YBL008W)|FD-Score:5.08|P-value:1.92E-7||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores Gene:HSL1(YKL101W)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p Gene:HSP26(YBR072W)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity Gene:IMP2'(YIL154C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:ITR2(YOL103W)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively Gene:LEU4(YNL104C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication Gene:MHT1(YLL062C)|FD-Score:3.77|P-value:8.28E-5||SGD DESC:S-methylmethionine-homocysteine methyltransferase, functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio Gene:MRH4(YGL064C)|FD-Score:-4.99|P-value:3.00E-7||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MRPS8(YMR158W)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MUK1(YPL070W)|FD-Score:5.13|P-value:1.49E-7||SGD DESC:Cytoplasmic protein of unknown function containing a Vps9 domain; computational analysis of large-scale protein-protein interaction data suggests a possible role in transcriptional regulation Gene:NRM1(YNR009W)|FD-Score:5.04|P-value:2.27E-7||SGD DESC:Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase Gene:NRT1(YOR071C)|FD-Score:-3.29|P-value:4.97E-4||SGD DESC:High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity Gene:PAN3(YKL025C)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PAN5(YHR063C)|FD-Score:-3.33|P-value:4.40E-4||SGD DESC:2-dehydropantoate 2-reductase, part of the pantothenic acid pathway, structurally homologous to E. coli panE Gene:PHB1(YGR132C)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation Gene:PHO88(YBR106W)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PTK1(YKL198C)|FD-Score:4.24|P-value:1.09E-5||SGD DESC:Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein Gene:PUS7(YOR243C)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RBG1(YAL036C)|FD-Score:4.67|P-value:1.49E-6||SGD DESC:Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid Gene:RDS1(YCR106W)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:RMD1(YDL001W)|FD-Score:3.75|P-value:8.97E-5||SGD DESC:Cytoplasmic protein required for sporulation Gene:RMD6(YEL072W)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Protein required for sporulation Gene:RPS10A(YOR293W)|FD-Score:-3.42|P-value:3.18E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication Gene:SAM4(YPL273W)|FD-Score:3.81|P-value:6.84E-5||SGD DESC:S-adenosylmethionine-homocysteine methyltransferase, functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio Gene:SAP30(YMR263W)|FD-Score:-3.77|P-value:8.28E-5||SGD DESC:Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance Gene:SDH2(YLL041C)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SGF73(YGL066W)|FD-Score:-3.24|P-value:5.93E-4||SGD DESC:SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SLX8(YER116C)|FD-Score:-3.48|P-value:2.55E-4||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:SPS18(YNL204C)|FD-Score:3.89|P-value:5.09E-5||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SRX1(YKL086W)|FD-Score:4.48|P-value:3.75E-6||SGD DESC:Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress Gene:STE20(YHL007C)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p Gene:SWS2(YNL081C)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TDA9(YML081W)|FD-Score:-3.23|P-value:6.17E-4||SGD DESC:DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication Gene:TIR3(YIL011W)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth Gene:TOK1(YJL093C)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin Gene:YAT1(YAR035W)|FD-Score:5.74|P-value:4.66E-9||SGD DESC:Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated Gene:YBR090C(YBR090C_p)|FD-Score:-3.11|P-value:9.48E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YCR006C(YCR006C_d)|FD-Score:4.08|P-value:2.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER077C(YER077C_p)|FD-Score:-4.15|P-value:1.67E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YGL218W(YGL218W_d)|FD-Score:-3.19|P-value:7.06E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance Gene:YML003W(YML003W_p)|FD-Score:4.93|P-value:4.05E-7||SGD DESC:Putative protein of unknown function Gene:YNL022C(YNL022C_p)|FD-Score:-3.33|P-value:4.39E-4||SGD DESC:Putative cytosine 5-methyltransferase, contains seven beta-strand methyltransferase motif similar to NOP2/YNL061W; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; predicted to be involved in ribosome biogenesis Gene:YOL037C(YOL037C_d)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W Gene:YPL114W(YPL114W_d)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL103W12.106.29E-347.92MET4Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p
YJR022W4.161.57E-50.19LSM8Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) that is part of spliceosomal U6 snRNP and is also implicated in processing of pre-tRNA, pre-snoRNA, and pre-rRNA
YMR197C3.973.62E-50.18VTI1Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways
YOR103C3.787.75E-50.20OST2Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
YDL153C3.581.71E-40.16SAS10Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance
YOR095C3.423.10E-40.32RKI1Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis
YBR055C3.119.46E-40.07PRP6Splicing factor, component of the U4/U6-U5 snRNP complex
YNL172W3.040.001180.16APC1Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress
YER006W2.880.002010.03NUG1GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus
YDR328C2.840.002220.04SKP1Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
YDR460W2.800.002520.01TFB3Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit
YBL018C2.790.002640.02POP8Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YGL099W2.770.002770.03LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YDR454C2.750.003000.11GUK1Guanylate kinase, converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteins
YDR013W2.640.004110.03PSF1Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YML099C7.242.27E-13ARG81Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p
YAR035W5.744.66E-9YAT1Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated
YPL070W5.131.49E-7MUK1Cytoplasmic protein of unknown function containing a Vps9 domain; computational analysis of large-scale protein-protein interaction data suggests a possible role in transcriptional regulation
YCL039W5.101.72E-7GID7Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions
YBL008W5.081.92E-7HIR1Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores
YNR009W5.042.27E-7NRM1Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase
YML003W_p4.934.05E-7YML003W_pPutative protein of unknown function
YAL036C4.671.49E-6RBG1Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid
YNL084C4.621.96E-6END3EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p
YKL086W4.483.75E-6SRX1Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress
YKL198C4.241.09E-5PTK1Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein
YCR106W4.161.58E-5RDS1Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide
YCR006C_d4.082.24E-5YCR006C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YOR243C4.042.62E-5PUS7Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria
YDR424C3.993.35E-5DYN2Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex

GO enrichment analysis for SGTC_3329
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1219.03E-21SGTC_33289140624 29.0 μMChembridge (Drug-like library)49043920.453125
0.1201.91E-20SGTC_33399145883 14.9 μMChembridge (Drug-like library)20306700.609375
0.1202.64E-20SGTC_14914138-0063 26.5 μMChemDiv (Drug-like library)238832270.123457
0.1132.92E-18SGTC_2803448-3607 3.0 μMChemDiv (Drug-like library)6261790.09195460S ribosome export
0.0921.70E-12SGTC_490phenamil 164.0 μMMiscellaneous47550.097561heme requiring
0.0815.74E-10SGTC_466geldanamycin 89.2 μMICCB bioactive library52883820.0512821RPP1 & pyrimidine depletion
0.0791.31E-9SGTC_11433454-2408 5.1 μMChemDiv (Drug-like library)X11430.28571460S ribosome export
0.0764.86E-9SGTC_2605digitonin 300.0 nMTimTec (Pure natural product library)27350100.037594
0.0756.45E-9SGTC_344k064-0035 6.4 μMChemDiv (Drug-like library)50887500.10810860S ribosome export
0.0741.25E-8SGTC_1786berberine 21.6 μMMiscellaneous124560.065217460S ribosome export
0.0708.60E-8SGTC_14053909-7894 12.0 μMChemDiv (Drug-like library)71040600.137560S ribosome export
0.0681.58E-7SGTC_12690831-0796 3.0 μMChemDiv (Drug-like library)9277580.112560S ribosome export
0.0663.38E-7SGTC_32879117173 37.1 μMChembridge (Drug-like library)27897090.15384660S ribosome export
0.0631.53E-6SGTC_2739tacrine 100.9 μMMiscellaneous19350.111111heme requiring
0.0621.85E-6SGTC_1690st023502 41.2 μMTimTec (Natural product derivative library)242074820.0721649calcium & mitochondrial duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3222912830149.47 μM0.754911358Chembridge (Drug-like library)375.390813.9330760S ribosome export
SGTC_334591484103.56 μM0.6393444903596Chembridge (Drug-like library)413.514783.9715
SGTC_3339914588314.94 μM0.6093752030670Chembridge (Drug-like library)379.498563.43515
SGTC_3326914006416.38 μM0.61868434Chembridge (Drug-like library)379.498563.42815
SGTC_3349914970323 μM0.5230774910494Chembridge (Drug-like library)351.44542.50915
SGTC_334891494643.54 μM0.51951442Chembridge (Drug-like library)379.498563.56915
SGTC_3340914663411.1 μM0.4714294907387Chembridge (Drug-like library)365.471983.14615
SGTC_3328914062429.04 μM0.4531254904392Chembridge (Drug-like library)335.4463.33104
SGTC_3343914837918.98 μM0.4347831955036Chembridge (Drug-like library)349.472583.96804
SGTC_3280914096149.47 μM0.4264714901226Chembridge (Drug-like library)355.864483.5090460S ribosome export