9141424

N-[2-(1H-indol-3-yl)ethyl]-6-methylthieno[2,3-d]pyrimidin-4-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3330
Screen concentration 71.4 μM
Source Chembridge (Drug-like library)
PubChem CID 27261020
SMILES CC1=CC2=C(N=CN=C2S1)NCCC3=CNC4=CC=CC=C43
Standardized SMILES Cc1cc2c(NCCc3c[nH]c4ccccc34)ncnc2s1
Molecular weight 308.4007
ALogP 3.8
H-bond donor count 2
H-bond acceptor count 4
Response signature amide catabolism

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.18
% growth inhibition (Hom. pool) 8.8


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 27261020
Download HIP data (tab-delimited text)  (excel)
Gene:DCP2(YNL118C)|FD-Score:3.49|P-value:2.40E-4|Clearance:0.1||SGD DESC:Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress Gene:GPI8(YDR331W)|FD-Score:-3.57|P-value:1.81E-4|Clearance:0||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:NOP4(YPL043W)|FD-Score:4.83|P-value:6.69E-7|Clearance:0.71||SGD DESC:Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) Gene:NUF2(YOL069W)|FD-Score:-3.09|P-value:9.98E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:POP6(YGR030C)|FD-Score:-3.18|P-value:7.26E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRE4(YFR050C)|FD-Score:4.13|P-value:1.85E-5|Clearance:0.4||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:PRP38(YGR075C)|FD-Score:-3.68|P-value:1.18E-4|Clearance:0||SGD DESC:Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly Gene:RLI1(YDR091C)|FD-Score:3.39|P-value:3.46E-4|Clearance:0.41||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RPC19(YNL113W)|FD-Score:-3.09|P-value:9.85E-4|Clearance:0||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RSE1(YML049C)|FD-Score:-3.11|P-value:9.37E-4|Clearance:0||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:TAF11(YML015C)|FD-Score:-3.66|P-value:1.27E-4|Clearance:0||SGD DESC:TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors Gene:TFG1(YGR186W)|FD-Score:3.5|P-value:2.31E-4|Clearance:0.01||SGD DESC:TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 Gene:UFD1(YGR048W)|FD-Score:-3.41|P-value:3.29E-4|Clearance:0||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP) Gene:UTP20(YBL004W)|FD-Score:3.73|P-value:9.55E-5|Clearance:0.13||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA Gene:YBL073W(YBL073W_d)|FD-Score:-3.84|P-value:6.05E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene AAR2/YBL074C Gene:YMR290W-A(YMR290W-A_d)|FD-Score:3.6|P-value:1.59E-4|Clearance:0.1||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YOR203W(YOR203W_d)|FD-Score:-3.66|P-value:1.28E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation Gene:YPT1(YFL038C)|FD-Score:-3.4|P-value:3.33E-4|Clearance:0||SGD DESC:Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p) Gene:DCP2(YNL118C)|FD-Score:3.49|P-value:2.40E-4|Clearance:0.1||SGD DESC:Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress Gene:GPI8(YDR331W)|FD-Score:-3.57|P-value:1.81E-4|Clearance:0||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:NOP4(YPL043W)|FD-Score:4.83|P-value:6.69E-7|Clearance:0.71||SGD DESC:Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) Gene:NUF2(YOL069W)|FD-Score:-3.09|P-value:9.98E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:POP6(YGR030C)|FD-Score:-3.18|P-value:7.26E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRE4(YFR050C)|FD-Score:4.13|P-value:1.85E-5|Clearance:0.4||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:PRP38(YGR075C)|FD-Score:-3.68|P-value:1.18E-4|Clearance:0||SGD DESC:Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly Gene:RLI1(YDR091C)|FD-Score:3.39|P-value:3.46E-4|Clearance:0.41||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RPC19(YNL113W)|FD-Score:-3.09|P-value:9.85E-4|Clearance:0||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RSE1(YML049C)|FD-Score:-3.11|P-value:9.37E-4|Clearance:0||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:TAF11(YML015C)|FD-Score:-3.66|P-value:1.27E-4|Clearance:0||SGD DESC:TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors Gene:TFG1(YGR186W)|FD-Score:3.5|P-value:2.31E-4|Clearance:0.01||SGD DESC:TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 Gene:UFD1(YGR048W)|FD-Score:-3.41|P-value:3.29E-4|Clearance:0||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP) Gene:UTP20(YBL004W)|FD-Score:3.73|P-value:9.55E-5|Clearance:0.13||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA Gene:YBL073W(YBL073W_d)|FD-Score:-3.84|P-value:6.05E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene AAR2/YBL074C Gene:YMR290W-A(YMR290W-A_d)|FD-Score:3.6|P-value:1.59E-4|Clearance:0.1||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YOR203W(YOR203W_d)|FD-Score:-3.66|P-value:1.28E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation Gene:YPT1(YFL038C)|FD-Score:-3.4|P-value:3.33E-4|Clearance:0||SGD DESC:Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 27261020
Download HOP data (tab-delimited text)  (excel)
Gene:ACH1(YBL015W)|FD-Score:-3.24|P-value:6.03E-4||SGD DESC:Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth Gene:ARC18(YLR370C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:BTS1(YPL069C)|FD-Score:-6.54|P-value:3.00E-11||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD19(YJL188C_d)|FD-Score:-5.16|P-value:1.24E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CDC73(YLR418C)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:COQ6(YGR255C)|FD-Score:-3.77|P-value:8.24E-5||SGD DESC:Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS) Gene:CSM2(YIL132C)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; required for accurate chromosome segregation during meiosis Gene:CWH41(YGL027C)|FD-Score:-4.33|P-value:7.63E-6||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:DAL81(YIR023W)|FD-Score:12.7|P-value:4.66E-37||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DAS1(YJL149W)|FD-Score:-3.12|P-value:9.10E-4||SGD DESC:Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C Gene:DNF3(YMR162C)|FD-Score:3.95|P-value:3.87E-5||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; localizes to the trans-Golgi network; likely involved in protein transport; type 4 P-type ATPase Gene:DPH5(YLR172C)|FD-Score:-3.54|P-value:2.04E-4||SGD DESC:Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm Gene:DUS4(YLR405W)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:ERG5(YMR015C)|FD-Score:6.06|P-value:6.90E-10||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:ETP1(YHL010C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Putative protein of unknown function that is required for growth on ethanol; contains a zinc finger region and has homology to human BRAP2, which is a cytoplasmic protein that binds nuclear localization sequences Gene:FET5(YFL041W)|FD-Score:-3.17|P-value:7.66E-4||SGD DESC:Multicopper oxidase, integral membrane protein with similarity to Fet3p; may have a role in iron transport Gene:FRE7(YOL152W)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels Gene:GEP4(YHR100C)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:HCH1(YNL281W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:HSP42(YDR171W)|FD-Score:4.42|P-value:4.92E-6||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:IES1(YFL013C)|FD-Score:-5.1|P-value:1.73E-7||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IME2(YJL106W)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Serine/threonine protein kinase involved in activation of meiosis, associates with Ime1p and mediates its stability, activates Ndt80p; IME2 expression is positively regulated by Ime1p Gene:IMH1(YLR309C)|FD-Score:5.42|P-value:2.90E-8||SGD DESC:Protein involved in vesicular transport, mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi Gene:KAP120(YPL125W)|FD-Score:3.3|P-value:4.75E-4||SGD DESC:Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p Gene:MRPL13(YKR006C)|FD-Score:5.22|P-value:9.07E-8||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPL31(YKL138C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB1(YOR188W)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:NCS2(YNL119W)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NCS6(YGL211W)|FD-Score:3.97|P-value:3.60E-5||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:OAF3(YKR064W)|FD-Score:-3.9|P-value:4.80E-5||SGD DESC:Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:OSH6(YKR003W)|FD-Score:3.8|P-value:7.34E-5||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:PDR3(YBL005W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome; PDR3 has a paralog, PDR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:PEF1(YGR058W)|FD-Score:-4.98|P-value:3.19E-7||SGD DESC:Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly Gene:PER33(YLR064W)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress Gene:PNC1(YGL037C)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Nicotinamidase that converts nicotinamide to nicotinic acid; part of the NAD(+) salvage pathway; required for life span extension by calorie restriction; PNC1 expression responds to all known stimuli that extend replicative life span; protein increases in abundance and relative distribution to cytoplasmic foci decreases upon DNA replication stress Gene:POS5(YPL188W)|FD-Score:-3.25|P-value:5.86E-4||SGD DESC:Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress Gene:PRM10(YJL108C)|FD-Score:-3.17|P-value:7.68E-4||SGD DESC:Pheromone-regulated protein, proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:PRO1(YDR300C)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication Gene:PSY2(YNL201C)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:RAD51(YER095W)|FD-Score:4.45|P-value:4.31E-6||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RPN4(YDL020C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTT107(YHR154W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SEC66(YBR171W)|FD-Score:8.81|P-value:6.24E-19||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SGE1(YPR198W)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SST2(YLR452C)|FD-Score:-3.21|P-value:6.63E-4||SGD DESC:GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family Gene:STP1(YDR463W)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:TAT1(YBR069C)|FD-Score:3.83|P-value:6.35E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:UBP14(YBR058C)|FD-Score:3.83|P-value:6.50E-5||SGD DESC:Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T Gene:UBP6(YFR010W)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:VAM6(YDL077C)|FD-Score:3.88|P-value:5.22E-5||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VBA1(YMR088C)|FD-Score:-3.11|P-value:9.29E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VMA16(YHR026W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VMA7(YGR020C)|FD-Score:3.24|P-value:6.04E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VMA8(YEL051W)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis Gene:VPS25(YJR102C)|FD-Score:-6.26|P-value:1.92E-10||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:YAF9(YNL107W)|FD-Score:7.92|P-value:1.21E-15||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YBR174C(YBR174C_d)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YCR087W(YCR087W_d)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Gene:YDL023C(YDL023C_d)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in other Saccharomyces species; overlaps the verified gene GPD1; deletion confers sensitivity to GSAO; deletion in cyr1 mutant results in loss of stress resistance Gene:YDL050C(YDL050C_d)|FD-Score:-3.42|P-value:3.16E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL129W(YDL129W_p)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress Gene:YDL241W(YDL241W_p)|FD-Score:4.55|P-value:2.63E-6||SGD DESC:Putative protein of unknown function; YDL241W is not an essential gene Gene:YDR222W(YDR222W_p)|FD-Score:-3.1|P-value:9.58E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YDR379C-A(YDR379C-A)|FD-Score:3.94|P-value:4.12E-5||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YDR509W(YDR509W_d)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFR012W-A(YFR012W-A_p)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Putative protein of unknown function; identified by homology Gene:YFR035C(YFR035C_p)|FD-Score:5.54|P-value:1.55E-8||SGD DESC:Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YGR151C(YGR151C_d)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YKL118W(YKL118W_d)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKL123W(YKL123W_d)|FD-Score:6.14|P-value:4.00E-10||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene SSH4 Gene:YNL162W-A(YNL162W-A_p)|FD-Score:-3.27|P-value:5.46E-4||SGD DESC:Putative protein of unknown function; identified by homology Gene:YOR012W(YOR012W_p)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Putative protein of unknown function Gene:YPR197C(YPR197C_d)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ACH1(YBL015W)|FD-Score:-3.24|P-value:6.03E-4||SGD DESC:Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth Gene:ARC18(YLR370C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:BTS1(YPL069C)|FD-Score:-6.54|P-value:3.00E-11||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD19(YJL188C_d)|FD-Score:-5.16|P-value:1.24E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CDC73(YLR418C)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:COQ6(YGR255C)|FD-Score:-3.77|P-value:8.24E-5||SGD DESC:Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS) Gene:CSM2(YIL132C)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; required for accurate chromosome segregation during meiosis Gene:CWH41(YGL027C)|FD-Score:-4.33|P-value:7.63E-6||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:DAL81(YIR023W)|FD-Score:12.7|P-value:4.66E-37||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DAS1(YJL149W)|FD-Score:-3.12|P-value:9.10E-4||SGD DESC:Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C Gene:DNF3(YMR162C)|FD-Score:3.95|P-value:3.87E-5||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; localizes to the trans-Golgi network; likely involved in protein transport; type 4 P-type ATPase Gene:DPH5(YLR172C)|FD-Score:-3.54|P-value:2.04E-4||SGD DESC:Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm Gene:DUS4(YLR405W)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:ERG5(YMR015C)|FD-Score:6.06|P-value:6.90E-10||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:ETP1(YHL010C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Putative protein of unknown function that is required for growth on ethanol; contains a zinc finger region and has homology to human BRAP2, which is a cytoplasmic protein that binds nuclear localization sequences Gene:FET5(YFL041W)|FD-Score:-3.17|P-value:7.66E-4||SGD DESC:Multicopper oxidase, integral membrane protein with similarity to Fet3p; may have a role in iron transport Gene:FRE7(YOL152W)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels Gene:GEP4(YHR100C)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:HCH1(YNL281W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:HSP42(YDR171W)|FD-Score:4.42|P-value:4.92E-6||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:IES1(YFL013C)|FD-Score:-5.1|P-value:1.73E-7||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IME2(YJL106W)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Serine/threonine protein kinase involved in activation of meiosis, associates with Ime1p and mediates its stability, activates Ndt80p; IME2 expression is positively regulated by Ime1p Gene:IMH1(YLR309C)|FD-Score:5.42|P-value:2.90E-8||SGD DESC:Protein involved in vesicular transport, mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi Gene:KAP120(YPL125W)|FD-Score:3.3|P-value:4.75E-4||SGD DESC:Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p Gene:MRPL13(YKR006C)|FD-Score:5.22|P-value:9.07E-8||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPL31(YKL138C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB1(YOR188W)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:NCS2(YNL119W)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NCS6(YGL211W)|FD-Score:3.97|P-value:3.60E-5||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:OAF3(YKR064W)|FD-Score:-3.9|P-value:4.80E-5||SGD DESC:Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:OSH6(YKR003W)|FD-Score:3.8|P-value:7.34E-5||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:PDR3(YBL005W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome; PDR3 has a paralog, PDR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:PEF1(YGR058W)|FD-Score:-4.98|P-value:3.19E-7||SGD DESC:Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly Gene:PER33(YLR064W)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress Gene:PNC1(YGL037C)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Nicotinamidase that converts nicotinamide to nicotinic acid; part of the NAD(+) salvage pathway; required for life span extension by calorie restriction; PNC1 expression responds to all known stimuli that extend replicative life span; protein increases in abundance and relative distribution to cytoplasmic foci decreases upon DNA replication stress Gene:POS5(YPL188W)|FD-Score:-3.25|P-value:5.86E-4||SGD DESC:Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress Gene:PRM10(YJL108C)|FD-Score:-3.17|P-value:7.68E-4||SGD DESC:Pheromone-regulated protein, proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:PRO1(YDR300C)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication Gene:PSY2(YNL201C)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:RAD51(YER095W)|FD-Score:4.45|P-value:4.31E-6||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RPN4(YDL020C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTT107(YHR154W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SEC66(YBR171W)|FD-Score:8.81|P-value:6.24E-19||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SGE1(YPR198W)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SST2(YLR452C)|FD-Score:-3.21|P-value:6.63E-4||SGD DESC:GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family Gene:STP1(YDR463W)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:TAT1(YBR069C)|FD-Score:3.83|P-value:6.35E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:UBP14(YBR058C)|FD-Score:3.83|P-value:6.50E-5||SGD DESC:Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T Gene:UBP6(YFR010W)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:VAM6(YDL077C)|FD-Score:3.88|P-value:5.22E-5||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VBA1(YMR088C)|FD-Score:-3.11|P-value:9.29E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VMA16(YHR026W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VMA7(YGR020C)|FD-Score:3.24|P-value:6.04E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VMA8(YEL051W)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis Gene:VPS25(YJR102C)|FD-Score:-6.26|P-value:1.92E-10||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:YAF9(YNL107W)|FD-Score:7.92|P-value:1.21E-15||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YBR174C(YBR174C_d)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YCR087W(YCR087W_d)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Gene:YDL023C(YDL023C_d)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in other Saccharomyces species; overlaps the verified gene GPD1; deletion confers sensitivity to GSAO; deletion in cyr1 mutant results in loss of stress resistance Gene:YDL050C(YDL050C_d)|FD-Score:-3.42|P-value:3.16E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL129W(YDL129W_p)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress Gene:YDL241W(YDL241W_p)|FD-Score:4.55|P-value:2.63E-6||SGD DESC:Putative protein of unknown function; YDL241W is not an essential gene Gene:YDR222W(YDR222W_p)|FD-Score:-3.1|P-value:9.58E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YDR379C-A(YDR379C-A)|FD-Score:3.94|P-value:4.12E-5||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YDR509W(YDR509W_d)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFR012W-A(YFR012W-A_p)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Putative protein of unknown function; identified by homology Gene:YFR035C(YFR035C_p)|FD-Score:5.54|P-value:1.55E-8||SGD DESC:Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YGR151C(YGR151C_d)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YKL118W(YKL118W_d)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKL123W(YKL123W_d)|FD-Score:6.14|P-value:4.00E-10||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene SSH4 Gene:YNL162W-A(YNL162W-A_p)|FD-Score:-3.27|P-value:5.46E-4||SGD DESC:Putative protein of unknown function; identified by homology Gene:YOR012W(YOR012W_p)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Putative protein of unknown function Gene:YPR197C(YPR197C_d)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL043W4.836.69E-70.71NOP4Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)
YFR050C4.131.85E-50.40PRE4Beta 7 subunit of the 20S proteasome
YBL004W3.739.55E-50.13UTP20Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA
YMR290W-A_d3.601.59E-40.10YMR290W-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase
YGR186W3.502.31E-40.01TFG1TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74
YNL118C3.492.40E-40.10DCP2Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress
YDR091C3.393.46E-40.41RLI1Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species
YGR091W2.980.001430.12PRP31Splicing factor, component of the U4/U6-U5 snRNP complex
YJR022W2.860.002110.01LSM8Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) that is part of spliceosomal U6 snRNP and is also implicated in processing of pre-tRNA, pre-snoRNA, and pre-rRNA
YML077W2.850.002210.03BET5Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi
YFL039C2.820.002400.12ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YLR029C2.700.003420.09RPL15ARibosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
YJL002C2.620.004460.04OST1Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
YKL154W2.580.004950.03SRP102Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane
YNL158W2.550.005370.01PGA1Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIR023W12.704.66E-37DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YBR171W8.816.24E-19SEC66Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YNL107W7.921.21E-15YAF9Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain
YKL123W_d6.144.00E-10YKL123W_dDubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene SSH4
YMR015C6.066.90E-10ERG5C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs
YFR035C_p5.541.55E-8YFR035C_pPutative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein
YLR309C5.422.90E-8IMH1Protein involved in vesicular transport, mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi
YKR006C5.229.07E-8MRPL13Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation
YDL241W_p4.552.63E-6YDL241W_pPutative protein of unknown function; YDL241W is not an essential gene
YER095W4.454.31E-6RAD51Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein
YDR171W4.424.92E-6HSP42Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress
YBR174C_d4.261.03E-5YBR174C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective
YPR197C_d4.251.05E-5YPR197C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YJL106W4.201.33E-5IME2Serine/threonine protein kinase involved in activation of meiosis, associates with Ime1p and mediates its stability, activates Ndt80p; IME2 expression is positively regulated by Ime1p
YPR198W4.072.35E-5SGE1Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations

GO enrichment analysis for SGTC_3330
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2441.01E-80SGTC_486niguldipine 82.0 μMMiscellaneous12360.0873786amide catabolism
0.2226.70E-67SGTC_24605552655 174.2 μMMiscellaneous54019890.108108amide catabolism
0.1924.72E-50SGTC_28459003024 43.0 μMChembridge (Drug-like library)29901730.103896amide catabolism
0.1783.68E-43SGTC_3371315-0038 354.0 μMChemDiv (Drug-like library)16264370.103448amide catabolism
0.1672.45E-38SGTC_9903985-0011 41.3 μMChemDiv (Drug-like library)53995650.0909091amide catabolism
0.1651.66E-37SGTC_5694130-2812 160.0 μMChemDiv (Drug-like library)7435520.101449amide catabolism
0.1598.29E-35SGTC_10563448-1962 115.0 μMChemDiv (Drug-like library)7155070.111111amide catabolism
0.1562.43E-33SGTC_31689103419 49.5 μMChembridge (Drug-like library)166492560.12560S ribosome export
0.1484.05E-30SGTC_7003453-2283 142.0 μMChemDiv (Drug-like library)5656640.109589amide catabolism
0.1475.53E-30SGTC_2680butyl paraben 22.2 μMMicrosource (Natural product library)71840.0724638amide catabolism
0.1433.34E-28SGTC_32289131799 49.5 μMChembridge (Drug-like library)170158180.125amide catabolism
0.1402.38E-27SGTC_32259130819 49.5 μMChembridge (Drug-like library)45471210.128205amide catabolism
0.1396.10E-27SGTC_32379133362 49.5 μMChembridge (Drug-like library)438451370.101124
0.1375.24E-26SGTC_15081080-0625 72.9 μMChemDiv (Drug-like library)7671110.0657895
0.1353.08E-25SGTC_1759st043059 70.0 μMTimTec (Natural product derivative library)6694260.106667amide catabolism

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_9933909-875762.7 μM0.456144288324ChemDiv (Drug-like library)275.75664.01114
SGTC_9923909-8734162 μM0.4210534288319ChemDiv (Drug-like library)275.75664.01114
SGTC_1774st04837148.47 μM0.3833334242727TimTec (Natural product derivative library)318.415483.47412RNA processing & uracil transport
SGTC_1998st07579776.8 μM0.38095224208315TimTec (Natural product derivative library)260.33153.40722mitochondrial response to ROS
SGTC_452diindolylmethane8 μM0.3673473071ICCB bioactive library246.306464.39120RNA processing & uracil transport
SGTC_2588indole-3-carbinol100 μM0.3529413712Microsource (Natural product library)147.173861.51821
SGTC_60844-001319.84 μM0.3442621584900ChemDiv (Drug-like library)451.580888.7710
SGTC_8010866-002810.1 μM0.3424666849771ChemDiv (Drug-like library)347.436783.65326
SGTC_2017513292760.65 μM0.3333338367Chembridge (Fragment library)188.268842.45121
SGTC_1790st04571143.5 μM0.328767585119TimTec (Natural product derivative library)459.3848795.01539