9141820

2-methyl-7-(4-methylpiperazin-1-yl)-3-phenyl-5-propan-2-ylpyrazolo[1,5-a]pyrimidine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3333
Screen concentration 12.1 μM
Source Chembridge (Drug-like library)
PubChem CID 1939433
SMILES CC1=NN2C(=CC(=NC2=C1C3=CC=CC=C3)C(C)C)N4CCN(CC4)C
Standardized SMILES CC(C)c1cc(N2CCN(C)CC2)n3nc(C)c(c4ccccc4)c3n1
Molecular weight 349.4726
ALogP 3.97
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.52
% growth inhibition (Hom. pool) 7.83


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1939433
Download HIP data (tab-delimited text)  (excel)
Gene:CCT6(YDR188W)|FD-Score:-3.83|P-value:6.50E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:MCM2(YBL023C)|FD-Score:3.32|P-value:4.45E-4|Clearance:0.07||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:MCM5(YLR274W)|FD-Score:4.42|P-value:4.95E-6|Clearance:0.58||SGD DESC:Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase Gene:NBP1(YLR457C)|FD-Score:4.53|P-value:2.99E-6|Clearance:0.58||SGD DESC:Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication Gene:NSE3(YDR288W)|FD-Score:3.84|P-value:6.25E-5|Clearance:0.01||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:NUP57(YGR119C)|FD-Score:-3.57|P-value:1.81E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:PCF11(YDR228C)|FD-Score:-3.29|P-value:4.92E-4|Clearance:0||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:POL5(YEL055C)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.06||SGD DESC:DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA Gene:POP6(YGR030C)|FD-Score:3.62|P-value:1.46E-4|Clearance:0.09||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRP31(YGR091W)|FD-Score:3.83|P-value:6.50E-5|Clearance:0.2||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RIX1(YHR197W)|FD-Score:4.6|P-value:2.14E-6|Clearance:0.58||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RKI1(YOR095C)|FD-Score:5.04|P-value:2.31E-7|Clearance:0.58||SGD DESC:Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis Gene:RNA1(YMR235C)|FD-Score:3.83|P-value:6.41E-5|Clearance:0||SGD DESC:GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport Gene:RPB8(YOR224C)|FD-Score:-4.28|P-value:9.36E-6|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPS15(YOL040C)|FD-Score:-3.66|P-value:1.24E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:UFD1(YGR048W)|FD-Score:3.37|P-value:3.74E-4|Clearance:0.05||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP) Gene:YJU2(YKL095W)|FD-Score:3.47|P-value:2.60E-4|Clearance:0.1||SGD DESC:Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing Gene:YNL247W(YNL247W)|FD-Score:3.25|P-value:5.76E-4|Clearance:0.21||SGD DESC:Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments Gene:CCT6(YDR188W)|FD-Score:-3.83|P-value:6.50E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:MCM2(YBL023C)|FD-Score:3.32|P-value:4.45E-4|Clearance:0.07||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:MCM5(YLR274W)|FD-Score:4.42|P-value:4.95E-6|Clearance:0.58||SGD DESC:Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase Gene:NBP1(YLR457C)|FD-Score:4.53|P-value:2.99E-6|Clearance:0.58||SGD DESC:Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication Gene:NSE3(YDR288W)|FD-Score:3.84|P-value:6.25E-5|Clearance:0.01||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:NUP57(YGR119C)|FD-Score:-3.57|P-value:1.81E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:PCF11(YDR228C)|FD-Score:-3.29|P-value:4.92E-4|Clearance:0||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:POL5(YEL055C)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.06||SGD DESC:DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA Gene:POP6(YGR030C)|FD-Score:3.62|P-value:1.46E-4|Clearance:0.09||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRP31(YGR091W)|FD-Score:3.83|P-value:6.50E-5|Clearance:0.2||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RIX1(YHR197W)|FD-Score:4.6|P-value:2.14E-6|Clearance:0.58||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RKI1(YOR095C)|FD-Score:5.04|P-value:2.31E-7|Clearance:0.58||SGD DESC:Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis Gene:RNA1(YMR235C)|FD-Score:3.83|P-value:6.41E-5|Clearance:0||SGD DESC:GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport Gene:RPB8(YOR224C)|FD-Score:-4.28|P-value:9.36E-6|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPS15(YOL040C)|FD-Score:-3.66|P-value:1.24E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:UFD1(YGR048W)|FD-Score:3.37|P-value:3.74E-4|Clearance:0.05||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP) Gene:YJU2(YKL095W)|FD-Score:3.47|P-value:2.60E-4|Clearance:0.1||SGD DESC:Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing Gene:YNL247W(YNL247W)|FD-Score:3.25|P-value:5.76E-4|Clearance:0.21||SGD DESC:Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1939433
Download HOP data (tab-delimited text)  (excel)
Gene:APE3(YBR286W)|FD-Score:-3.22|P-value:6.44E-4||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:ATP14(YLR295C)|FD-Score:-3.29|P-value:5.03E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:AZF1(YOR113W)|FD-Score:4.57|P-value:2.48E-6||SGD DESC:Zinc-finger transcription factor; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity Gene:BMH1(YER177W)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:CCW12(YLR110C)|FD-Score:5.56|P-value:1.33E-8||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CDC26(YFR036W)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:COG8(YML071C)|FD-Score:-4.25|P-value:1.08E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSM1(YCR086W)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:ERG4(YGL012W)|FD-Score:-3.66|P-value:1.24E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERV14(YGL054C)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:EXG2(YDR261C)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:EXO5(YBR163W)|FD-Score:-3.36|P-value:3.87E-4||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:FET4(YMR319C)|FD-Score:-3.92|P-value:4.47E-5||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FRE7(YOL152W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels Gene:FYV12(YOR183W_p)|FD-Score:-4.24|P-value:1.12E-5||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:FYV5(YCL058C)|FD-Score:-3.84|P-value:6.21E-5||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:GIP3(YPL137C)|FD-Score:-3.72|P-value:9.83E-5||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:HCM1(YCR065W)|FD-Score:4.98|P-value:3.24E-7||SGD DESC:Forkhead transcription factor that drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role Gene:HLJ1(YMR161W)|FD-Score:3.12|P-value:9.04E-4||SGD DESC:Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ Gene:HOR7(YMR251W-A)|FD-Score:3.88|P-value:5.20E-5||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:HXK2(YGL253W)|FD-Score:-4.22|P-value:1.22E-5||SGD DESC:Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication Gene:IDP1(YDL066W)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IRC23(YOR044W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication Gene:LTP1(YPR073C)|FD-Score:3.77|P-value:8.25E-5||SGD DESC:Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine Gene:MNN11(YJL183W)|FD-Score:4.32|P-value:7.83E-6||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MRPL31(YKL138C)|FD-Score:-3.3|P-value:4.79E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL6(YHR147C)|FD-Score:-4.49|P-value:3.48E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB2(YGR014W)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:MSD1(YPL104W)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:MSY1(YPL097W)|FD-Score:-4.06|P-value:2.47E-5||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:NCA3(YJL116C)|FD-Score:3.81|P-value:7.09E-5||SGD DESC:Protein involved in mitochondrion organization; functions with Nca2p to regulate mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; member of the SUN family; expression induced in cells treated with the mycotoxin patulin; NCA3 has a paralog, UTH1, that arose from the whole genome duplication Gene:NEM1(YHR004C)|FD-Score:-3.79|P-value:7.57E-5||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:NUP53(YMR153W)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication Gene:OPI8(YKR035C_d)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:OPY2(YPR075C)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress Gene:PAN2(YGL094C)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PCL7(YIL050W)|FD-Score:-4.53|P-value:2.94E-6||SGD DESC:Pho85p cyclin of the Pho80p subfamily, forms a functional kinase complex with Pho85p which phosphorylates Mmr1p and is regulated by Pho81p; involved in glycogen metabolism, expression is cell-cycle regulated Gene:PDA1(YER178W)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose Gene:PIH1(YHR034C)|FD-Score:-4.41|P-value:5.27E-6||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PIN3(YPR154W)|FD-Score:-3.29|P-value:4.93E-4||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced Gene:POM34(YLR018C)|FD-Score:-3.16|P-value:7.88E-4||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication Gene:PPT2(YPL148C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PRR1(YKL116C)|FD-Score:-3.77|P-value:8.28E-5||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor Gene:PRY2(YKR013W)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:RPL14B(YHL001W)|FD-Score:-3.1|P-value:9.59E-4||SGD DESC:Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SLX8(YER116C)|FD-Score:6.32|P-value:1.34E-10||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:SLZ1(YNL196C_p)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SNF5(YBR289W)|FD-Score:-5.02|P-value:2.57E-7||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SSA3(YBL075C)|FD-Score:6.17|P-value:3.36E-10||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:SSP120(YLR250W)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SUT2(YPR009W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Putative transcription factor; multicopy suppressor of mutations that cause low activity of the cAMP/protein kinase A pathway; highly similar to Sut1p Gene:TCA17(YEL048C)|FD-Score:-3.17|P-value:7.74E-4||SGD DESC:Subunit of TRAPPII, a multimeric GEF involved in intra-Golgi and endosome-to-Golgi transport; promotes association of TRAPPII-specific subunits with the core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder Gene:TPA1(YER049W)|FD-Score:-5.59|P-value:1.12E-8||SGD DESC:Poly(rA)-binding protein involved in translation termination efficiency, mRNA poly(A) tail length and mRNA stability; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to prolyl 4-hydroxylases; binds Fe(III) and 2-oxoglutarate Gene:TVP18(YMR071C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:VPS29(YHR012W)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:YBR053C(YBR053C_p)|FD-Score:5.06|P-value:2.14E-7||SGD DESC:Putative protein of unknown function; induced by cell wall perturbation Gene:YBR063C(YBR063C_p)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Putative protein of unknown function; YBR063C is not an essential gene Gene:YCP4(YCR004C)|FD-Score:5.93|P-value:1.50E-9||SGD DESC:Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YCR100C(YCR100C_p)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Putative protein of unknown function Gene:YDR048C(YDR048C_d)|FD-Score:-3.62|P-value:1.47E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR509W(YDR509W_d)|FD-Score:-5.04|P-value:2.34E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR537C(YDR537C_d)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W Gene:YFL013W-A(YFL013W-A_d)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching Gene:YFR035C(YFR035C_p)|FD-Score:-4.34|P-value:7.15E-6||SGD DESC:Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YGL082W(YGL082W_p)|FD-Score:-5.39|P-value:3.51E-8||SGD DESC:Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YGL082W is not an essential gene Gene:YGR050C(YGR050C_p)|FD-Score:5.64|P-value:8.43E-9||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGR137W(YGR137W_d)|FD-Score:3.87|P-value:5.39E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR087W(YJR087W_d)|FD-Score:-3.27|P-value:5.30E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified genes STE18 and ECM2 Gene:YLR126C(YLR126C_p)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Putative glutamine amidotransferase; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:YMR084W(YMR084W_p)|FD-Score:6.33|P-value:1.24E-10||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YNL205C(YNL205C_d)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL159C-A(YOL159C-A)|FD-Score:-3.31|P-value:4.64E-4||SGD DESC:Protein of unknown function; overexpression affects endocytic protein trafficking; identified by sequence comparison with hemiascomycetous yeast species Gene:YOR121C(YOR121C_d)|FD-Score:-3.54|P-value:1.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YPL041C(YPL041C_p)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Protein of unknown function involved in maintenance of proper telomere length Gene:YPR109W(YPR109W_p)|FD-Score:5.35|P-value:4.37E-8||SGD DESC:Predicted membrane protein; dipoid deletion strain has high budding index Gene:APE3(YBR286W)|FD-Score:-3.22|P-value:6.44E-4||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:ATP14(YLR295C)|FD-Score:-3.29|P-value:5.03E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:AZF1(YOR113W)|FD-Score:4.57|P-value:2.48E-6||SGD DESC:Zinc-finger transcription factor; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity Gene:BMH1(YER177W)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:CCW12(YLR110C)|FD-Score:5.56|P-value:1.33E-8||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CDC26(YFR036W)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:COG8(YML071C)|FD-Score:-4.25|P-value:1.08E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSM1(YCR086W)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:ERG4(YGL012W)|FD-Score:-3.66|P-value:1.24E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERV14(YGL054C)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:EXG2(YDR261C)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:EXO5(YBR163W)|FD-Score:-3.36|P-value:3.87E-4||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:FET4(YMR319C)|FD-Score:-3.92|P-value:4.47E-5||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FRE7(YOL152W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels Gene:FYV12(YOR183W_p)|FD-Score:-4.24|P-value:1.12E-5||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:FYV5(YCL058C)|FD-Score:-3.84|P-value:6.21E-5||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:GIP3(YPL137C)|FD-Score:-3.72|P-value:9.83E-5||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:HCM1(YCR065W)|FD-Score:4.98|P-value:3.24E-7||SGD DESC:Forkhead transcription factor that drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role Gene:HLJ1(YMR161W)|FD-Score:3.12|P-value:9.04E-4||SGD DESC:Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ Gene:HOR7(YMR251W-A)|FD-Score:3.88|P-value:5.20E-5||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:HXK2(YGL253W)|FD-Score:-4.22|P-value:1.22E-5||SGD DESC:Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication Gene:IDP1(YDL066W)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IRC23(YOR044W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication Gene:LTP1(YPR073C)|FD-Score:3.77|P-value:8.25E-5||SGD DESC:Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine Gene:MNN11(YJL183W)|FD-Score:4.32|P-value:7.83E-6||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MRPL31(YKL138C)|FD-Score:-3.3|P-value:4.79E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL6(YHR147C)|FD-Score:-4.49|P-value:3.48E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB2(YGR014W)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:MSD1(YPL104W)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:MSY1(YPL097W)|FD-Score:-4.06|P-value:2.47E-5||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:NCA3(YJL116C)|FD-Score:3.81|P-value:7.09E-5||SGD DESC:Protein involved in mitochondrion organization; functions with Nca2p to regulate mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; member of the SUN family; expression induced in cells treated with the mycotoxin patulin; NCA3 has a paralog, UTH1, that arose from the whole genome duplication Gene:NEM1(YHR004C)|FD-Score:-3.79|P-value:7.57E-5||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:NUP53(YMR153W)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication Gene:OPI8(YKR035C_d)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:OPY2(YPR075C)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress Gene:PAN2(YGL094C)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PCL7(YIL050W)|FD-Score:-4.53|P-value:2.94E-6||SGD DESC:Pho85p cyclin of the Pho80p subfamily, forms a functional kinase complex with Pho85p which phosphorylates Mmr1p and is regulated by Pho81p; involved in glycogen metabolism, expression is cell-cycle regulated Gene:PDA1(YER178W)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose Gene:PIH1(YHR034C)|FD-Score:-4.41|P-value:5.27E-6||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PIN3(YPR154W)|FD-Score:-3.29|P-value:4.93E-4||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced Gene:POM34(YLR018C)|FD-Score:-3.16|P-value:7.88E-4||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication Gene:PPT2(YPL148C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PRR1(YKL116C)|FD-Score:-3.77|P-value:8.28E-5||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor Gene:PRY2(YKR013W)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:RPL14B(YHL001W)|FD-Score:-3.1|P-value:9.59E-4||SGD DESC:Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SLX8(YER116C)|FD-Score:6.32|P-value:1.34E-10||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:SLZ1(YNL196C_p)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SNF5(YBR289W)|FD-Score:-5.02|P-value:2.57E-7||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SSA3(YBL075C)|FD-Score:6.17|P-value:3.36E-10||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:SSP120(YLR250W)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SUT2(YPR009W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Putative transcription factor; multicopy suppressor of mutations that cause low activity of the cAMP/protein kinase A pathway; highly similar to Sut1p Gene:TCA17(YEL048C)|FD-Score:-3.17|P-value:7.74E-4||SGD DESC:Subunit of TRAPPII, a multimeric GEF involved in intra-Golgi and endosome-to-Golgi transport; promotes association of TRAPPII-specific subunits with the core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder Gene:TPA1(YER049W)|FD-Score:-5.59|P-value:1.12E-8||SGD DESC:Poly(rA)-binding protein involved in translation termination efficiency, mRNA poly(A) tail length and mRNA stability; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to prolyl 4-hydroxylases; binds Fe(III) and 2-oxoglutarate Gene:TVP18(YMR071C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:VPS29(YHR012W)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:YBR053C(YBR053C_p)|FD-Score:5.06|P-value:2.14E-7||SGD DESC:Putative protein of unknown function; induced by cell wall perturbation Gene:YBR063C(YBR063C_p)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Putative protein of unknown function; YBR063C is not an essential gene Gene:YCP4(YCR004C)|FD-Score:5.93|P-value:1.50E-9||SGD DESC:Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YCR100C(YCR100C_p)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Putative protein of unknown function Gene:YDR048C(YDR048C_d)|FD-Score:-3.62|P-value:1.47E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR509W(YDR509W_d)|FD-Score:-5.04|P-value:2.34E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR537C(YDR537C_d)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W Gene:YFL013W-A(YFL013W-A_d)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching Gene:YFR035C(YFR035C_p)|FD-Score:-4.34|P-value:7.15E-6||SGD DESC:Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YGL082W(YGL082W_p)|FD-Score:-5.39|P-value:3.51E-8||SGD DESC:Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YGL082W is not an essential gene Gene:YGR050C(YGR050C_p)|FD-Score:5.64|P-value:8.43E-9||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGR137W(YGR137W_d)|FD-Score:3.87|P-value:5.39E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR087W(YJR087W_d)|FD-Score:-3.27|P-value:5.30E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified genes STE18 and ECM2 Gene:YLR126C(YLR126C_p)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Putative glutamine amidotransferase; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:YMR084W(YMR084W_p)|FD-Score:6.33|P-value:1.24E-10||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YNL205C(YNL205C_d)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL159C-A(YOL159C-A)|FD-Score:-3.31|P-value:4.64E-4||SGD DESC:Protein of unknown function; overexpression affects endocytic protein trafficking; identified by sequence comparison with hemiascomycetous yeast species Gene:YOR121C(YOR121C_d)|FD-Score:-3.54|P-value:1.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YPL041C(YPL041C_p)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Protein of unknown function involved in maintenance of proper telomere length Gene:YPR109W(YPR109W_p)|FD-Score:5.35|P-value:4.37E-8||SGD DESC:Predicted membrane protein; dipoid deletion strain has high budding index

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR095C5.042.31E-70.58RKI1Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis
YHR197W4.602.14E-60.58RIX1Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene
YLR457C4.532.99E-60.58NBP1Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication
YLR274W4.424.95E-60.58MCM5Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase
YDR288W3.846.25E-50.01NSE3Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress
YMR235C3.836.41E-50.00RNA1GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport
YGR091W3.836.50E-50.20PRP31Splicing factor, component of the U4/U6-U5 snRNP complex
YGR030C3.621.46E-40.09POP6Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YEL055C3.532.09E-40.06POL5DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA
YKL095W3.472.60E-40.10YJU2Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing
YGR048W3.373.74E-40.05UFD1Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP)
YBL023C3.324.45E-40.07MCM2Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress
YNL247W3.255.76E-40.21YNL247WCysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments
YDR177W3.040.001170.14UBC1Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress
YBR234C2.900.001880.02ARC40Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR084W_p6.331.24E-10YMR084W_pPutative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1
YER116C6.321.34E-10SLX8Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress
YBL075C6.173.36E-10SSA3ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication
YCR004C5.931.50E-9YCP4Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YGR050C_p5.648.43E-9YGR050C_pProtein of unknown function; mRNA identified as translated by ribosome profiling data
YLR110C5.561.33E-8CCW12Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication
YPR109W_p5.354.37E-8YPR109W_pPredicted membrane protein; dipoid deletion strain has high budding index
YBR053C_p5.062.14E-7YBR053C_pPutative protein of unknown function; induced by cell wall perturbation
YCR065W4.983.24E-7HCM1Forkhead transcription factor that drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role
YOR113W4.572.48E-6AZF1Zinc-finger transcription factor; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity
YJL183W4.327.83E-6MNN11Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p
YNL196C_p4.211.28E-5SLZ1_pSporulation-specific protein with a leucine zipper motif
YGL094C4.181.43E-5PAN2Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes
YMR251W-A3.885.20E-5HOR7Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication
YGR137W_d3.875.39E-5YGR137W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_3333
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0781.69E-9SGTC_31259127534 49.5 μMChembridge (Drug-like library)16392350.08
0.0715.71E-8SGTC_1949st076024 69.1 μMTimTec (Natural product derivative library)29219300.0843373
0.0656.50E-7SGTC_2778tioconazole 65.1 nMMiscellaneous54820.0813954ergosterol depletion effects on membrane
0.0603.87E-6SGTC_2170morpholine 200.0 μMChembridge (Fragment library)1211710.128571
0.0594.79E-6SGTC_30299088683 49.5 μMChembridge (Drug-like library)252366150.166667
0.0561.96E-5SGTC_33299141233 27.6 μMChembridge (Drug-like library)49091010.426471
0.0542.80E-5SGTC_12690831-0796 3.0 μMChemDiv (Drug-like library)9277580.12328860S ribosome export
0.0534.07E-5SGTC_460trequinsin 123.0 μMMiscellaneous55370.107143
0.0526.46E-5SGTC_22687933382 153.8 μMChembridge (Fragment library)9350250.123288
0.0526.99E-5SGTC_21205538279 152.0 μMChembridge (Fragment library)20581970.056338
0.0527.35E-5SGTC_22016605497 169.9 μMChembridge (Fragment library)6707410.171875ergosterol biosynthesis
0.0518.36E-5SGTC_774k060-0086 136.0 μMChemDiv (Drug-like library)59112350.105263
0.0518.35E-5SGTC_29729093619 59.9 μMChembridge (Drug-like library)252362560.0909091
0.0501.10E-4SGTC_32509135583 49.5 μMChembridge (Drug-like library)76993530.08
0.0501.39E-4SGTC_12212958-7315 257.0 μMChemDiv (Drug-like library)45627050.0526316

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3339914588314.94 μM0.7454552030670Chembridge (Drug-like library)379.498563.43515
SGTC_3343914837918.98 μM0.6727271955036Chembridge (Drug-like library)349.472583.96804
SGTC_3328914062429.04 μM0.6226424904392Chembridge (Drug-like library)335.4463.33104
SGTC_3222912830149.47 μM0.5689654911358Chembridge (Drug-like library)375.390813.9330760S ribosome export
SGTC_3280914096149.47 μM0.5517244901226Chembridge (Drug-like library)355.864483.5090460S ribosome export
SGTC_334591484103.56 μM0.554903596Chembridge (Drug-like library)413.514783.9715
SGTC_3319913845016.76 μM0.5230772029690Chembridge (Drug-like library)379.498563.4915
SGTC_3326914006416.38 μM0.5156251868434Chembridge (Drug-like library)379.498563.42815
SGTC_3235913260249.47 μM0.4285714912240Chembridge (Drug-like library)352.430163.31605
SGTC_3329914123327.56 μM0.4264714909101Chembridge (Drug-like library)405.416793.39418