9145883

2-[4-(2-methyl-3-phenyl-5-propan-2-ylpyrazolo[1,5-a]pyrimidin-7-yl)piperazin-1-yl]ethanol

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3339
Screen concentration 14.9 μM
Source Chembridge (Drug-like library)
PubChem CID 2030670
SMILES CC1=NN2C(=CC(=NC2=C1C3=CC=CC=C3)C(C)C)N4CCN(CC4)CCO
Standardized SMILES CC(C)c1cc(N2CCN(CCO)CC2)n3nc(C)c(c4ccccc4)c3n1
Molecular weight 379.4986
ALogP 3.44
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 17.45
% growth inhibition (Hom. pool) 14.79


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2030670
Download HIP data (tab-delimited text)  (excel)
Gene:CCT3(YJL014W)|FD-Score:-3.09|P-value:9.92E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT6(YDR188W)|FD-Score:-3.36|P-value:3.91E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CCT7(YJL111W)|FD-Score:-3.9|P-value:4.78E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC12(YHR107C)|FD-Score:3.35|P-value:4.03E-4|Clearance:0.24||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:DED1(YOR204W)|FD-Score:3.73|P-value:9.45E-5|Clearance:0.38||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:IRA1(YBR140C)|FD-Score:-3.87|P-value:5.48E-5|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:MAS1(YLR163C)|FD-Score:-3.48|P-value:2.52E-4|Clearance:0||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:NDC1(YML031W)|FD-Score:-3.23|P-value:6.10E-4|Clearance:0||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:PRP6(YBR055C)|FD-Score:3.11|P-value:9.37E-4|Clearance:0.02||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPB3(YIL021W)|FD-Score:-3.67|P-value:1.19E-4|Clearance:0||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPC10(YHR143W-A)|FD-Score:-3.55|P-value:1.96E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:RPL17A(YKL180W)|FD-Score:-4.41|P-value:5.24E-6|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL18A(YOL120C)|FD-Score:-3.79|P-value:7.59E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPL25(YOL127W)|FD-Score:-4.64|P-value:1.78E-6|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPL33A(YPL143W)|FD-Score:-4.04|P-value:2.65E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:RPO26(YPR187W)|FD-Score:-3.54|P-value:2.02E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPS13(YDR064W)|FD-Score:-3.5|P-value:2.34E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPS15(YOL040C)|FD-Score:-4.38|P-value:6.00E-6|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPS20(YHL015W)|FD-Score:-4.1|P-value:2.07E-5|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS5(YJR123W)|FD-Score:-3.53|P-value:2.09E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:SWD2(YKL018W)|FD-Score:-3.28|P-value:5.27E-4|Clearance:0||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination Gene:UTP5(YDR398W)|FD-Score:-3.27|P-value:5.43E-4|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YDL152W(YDL152W_d)|FD-Score:-4.09|P-value:2.20E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YDL196W(YDL196W_d)|FD-Score:-3.15|P-value:8.17E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YPL142C(YPL142C_d)|FD-Score:-3.61|P-value:1.54E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Gene:CCT3(YJL014W)|FD-Score:-3.09|P-value:9.92E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT6(YDR188W)|FD-Score:-3.36|P-value:3.91E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CCT7(YJL111W)|FD-Score:-3.9|P-value:4.78E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC12(YHR107C)|FD-Score:3.35|P-value:4.03E-4|Clearance:0.24||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:DED1(YOR204W)|FD-Score:3.73|P-value:9.45E-5|Clearance:0.38||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:IRA1(YBR140C)|FD-Score:-3.87|P-value:5.48E-5|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:MAS1(YLR163C)|FD-Score:-3.48|P-value:2.52E-4|Clearance:0||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:NDC1(YML031W)|FD-Score:-3.23|P-value:6.10E-4|Clearance:0||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:PRP6(YBR055C)|FD-Score:3.11|P-value:9.37E-4|Clearance:0.02||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPB3(YIL021W)|FD-Score:-3.67|P-value:1.19E-4|Clearance:0||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPC10(YHR143W-A)|FD-Score:-3.55|P-value:1.96E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:RPL17A(YKL180W)|FD-Score:-4.41|P-value:5.24E-6|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL18A(YOL120C)|FD-Score:-3.79|P-value:7.59E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPL25(YOL127W)|FD-Score:-4.64|P-value:1.78E-6|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPL33A(YPL143W)|FD-Score:-4.04|P-value:2.65E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:RPO26(YPR187W)|FD-Score:-3.54|P-value:2.02E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPS13(YDR064W)|FD-Score:-3.5|P-value:2.34E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPS15(YOL040C)|FD-Score:-4.38|P-value:6.00E-6|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPS20(YHL015W)|FD-Score:-4.1|P-value:2.07E-5|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS5(YJR123W)|FD-Score:-3.53|P-value:2.09E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:SWD2(YKL018W)|FD-Score:-3.28|P-value:5.27E-4|Clearance:0||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination Gene:UTP5(YDR398W)|FD-Score:-3.27|P-value:5.43E-4|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YDL152W(YDL152W_d)|FD-Score:-4.09|P-value:2.20E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YDL196W(YDL196W_d)|FD-Score:-3.15|P-value:8.17E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YPL142C(YPL142C_d)|FD-Score:-3.61|P-value:1.54E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2030670
Download HOP data (tab-delimited text)  (excel)
Gene:ASC1(YMR116C)|FD-Score:3.73|P-value:9.52E-5||SGD DESC:G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation Gene:BMH1(YER177W)|FD-Score:4.01|P-value:3.06E-5||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:CLB3(YDL155W)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress Gene:CLB5(YPR120C)|FD-Score:-3.28|P-value:5.24E-4||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase Gene:COQ10(YOL008W)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:CPR7(YJR032W)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:DDR2(YOL052C-A)|FD-Score:3.37|P-value:3.69E-4||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:DEP1(YAL013W)|FD-Score:-3.94|P-value:4.00E-5||SGD DESC:Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation Gene:DPH5(YLR172C)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm Gene:DUR3(YHL016C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway Gene:ELM1(YKL048C)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:EPS1(YIL005W)|FD-Score:3.23|P-value:6.13E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:ERP1(YAR002C-A)|FD-Score:5.38|P-value:3.67E-8||SGD DESC:Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication Gene:FKH1(YIL131C)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Forkhead family transcription factor; has a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; binds to the recombination enhancer near HML, and regulates donor preference during mating-type switching by positioning HML near the MAT locus through FHA-domain mediated interactions with proteins near the DSB; FKH1 has a paralog, FKH2, that arose from the whole genome duplication Gene:FYV12(YOR183W_p)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GET3(YDL100C)|FD-Score:-5.82|P-value:2.95E-9||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GLG1(YKR058W)|FD-Score:4.5|P-value:3.43E-6||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG1 has a paralog, GLG2, that arose from the whole genome duplication Gene:GYP6(YJL044C)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport Gene:HAL1(YPR005C)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p Gene:HST1(YOL068C)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:HUR1(YGL168W)|FD-Score:-3.17|P-value:7.63E-4||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:IZH2(YOL002C)|FD-Score:4.48|P-value:3.69E-6||SGD DESC:Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc Gene:LIN1(YHR156C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication Gene:MAK3(YPR051W)|FD-Score:-3.26|P-value:5.56E-4||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:MDS3(YGL197W)|FD-Score:3.74|P-value:9.16E-5||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MKT1(YNL085W)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MNN2(YBR015C)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRM2(YGL136C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Mitochondrial 2' O-ribose methyltransferase, required for methylation of U(2791) in 21S rRNA; MRM2 deletion confers thermosensitive respiration and loss of mitochondrial DNA; has similarity to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ Gene:MTC2(YKL098W)|FD-Score:5.18|P-value:1.11E-7||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:NPT1(YOR209C)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:OTU2(YHL013C)|FD-Score:3.12|P-value:8.89E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress Gene:PAN3(YKL025C)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PEX28(YHR150W)|FD-Score:-3.26|P-value:5.50E-4||SGD DESC:Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p Gene:PKR1(YMR123W)|FD-Score:-3.31|P-value:4.71E-4||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PPM1(YDR435C)|FD-Score:-3.64|P-value:1.34E-4||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PTK2(YJR059W)|FD-Score:-3.27|P-value:5.31E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PUS7(YOR243C)|FD-Score:5.02|P-value:2.54E-7||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:QRI5(YLR204W)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:REG1(YDR028C)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:REH1(YLR387C)|FD-Score:7.66|P-value:9.03E-15||SGD DESC:Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains Gene:RIF2(YLR453C)|FD-Score:4.66|P-value:1.57E-6||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RMD1(YDL001W)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Cytoplasmic protein required for sporulation Gene:RPL35B(YDL136W)|FD-Score:-5.31|P-value:5.54E-8||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RSR1(YGR152C)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases Gene:RTT103(YDR289C)|FD-Score:-3.1|P-value:9.66E-4||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:RXT2(YBR095C)|FD-Score:-3.54|P-value:2.03E-4||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SDP1(YIL113W)|FD-Score:-3.28|P-value:5.20E-4||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SGE1(YPR198W)|FD-Score:-5.51|P-value:1.78E-8||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SIN3(YOL004W)|FD-Score:-3.36|P-value:3.87E-4||SGD DESC:Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity Gene:SIP5(YMR140W)|FD-Score:3.13|P-value:8.70E-4||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:SKY1(YMR216C)|FD-Score:-3.4|P-value:3.42E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SLH1(YGR271W)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SLP1(YOR154W_p)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p Gene:SNQ2(YDR011W)|FD-Score:3.88|P-value:5.24E-5||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SPT2(YER161C)|FD-Score:5.63|P-value:8.81E-9||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SPT4(YGR063C)|FD-Score:-3.8|P-value:7.20E-5||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SPT7(YBR081C)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:SSF1(YHR066W)|FD-Score:-3.33|P-value:4.36E-4||SGD DESC:Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication Gene:STP4(YDL048C)|FD-Score:-3.94|P-value:4.02E-5||SGD DESC:Protein containing a Kruppel-type zinc-finger domain; has similarity to Stp1p, Stp2p, and Stp3p; predicted to be a transcription factor; relative distribution to the nucleus increases upon DNA replication stress Gene:SUV3(YPL029W)|FD-Score:-5.28|P-value:6.37E-8||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TPA1(YER049W)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:Poly(rA)-binding protein involved in translation termination efficiency, mRNA poly(A) tail length and mRNA stability; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to prolyl 4-hydroxylases; binds Fe(III) and 2-oxoglutarate Gene:TRP1(YDR007W)|FD-Score:-3.36|P-value:3.92E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:UBP13(YBL067C)|FD-Score:-3.39|P-value:3.52E-4||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:UBP6(YFR010W)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:VMA1(YDL185W)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA9(YCL005W-A)|FD-Score:-3.27|P-value:5.36E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:YAL042C-A(YAL042C-A_d)|FD-Score:4.02|P-value:2.94E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Gene:YCR085W(YCR085W_d)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR034W-B(YDR034W-B_p)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR290W(YDR290W_d)|FD-Score:-4.09|P-value:2.14E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YFL012W(YFL012W_p)|FD-Score:-3.35|P-value:3.99E-4||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YGL214W(YGL214W_d)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C Gene:YGR111W(YGR111W_p)|FD-Score:-4.07|P-value:2.32E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YGR273C(YGR273C_p)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YHL008C(YHL008C_p)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YJL193W(YJL193W_p)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect Gene:YOL099C(YOL099C_d)|FD-Score:3.11|P-value:9.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes Gene:YPK1(YKL126W)|FD-Score:-5.36|P-value:4.27E-8||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR147C(YPR147C_p)|FD-Score:3.83|P-value:6.28E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:ASC1(YMR116C)|FD-Score:3.73|P-value:9.52E-5||SGD DESC:G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation Gene:BMH1(YER177W)|FD-Score:4.01|P-value:3.06E-5||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:CLB3(YDL155W)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress Gene:CLB5(YPR120C)|FD-Score:-3.28|P-value:5.24E-4||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase Gene:COQ10(YOL008W)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:CPR7(YJR032W)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:DDR2(YOL052C-A)|FD-Score:3.37|P-value:3.69E-4||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:DEP1(YAL013W)|FD-Score:-3.94|P-value:4.00E-5||SGD DESC:Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation Gene:DPH5(YLR172C)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm Gene:DUR3(YHL016C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway Gene:ELM1(YKL048C)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:EPS1(YIL005W)|FD-Score:3.23|P-value:6.13E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:ERP1(YAR002C-A)|FD-Score:5.38|P-value:3.67E-8||SGD DESC:Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication Gene:FKH1(YIL131C)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Forkhead family transcription factor; has a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; binds to the recombination enhancer near HML, and regulates donor preference during mating-type switching by positioning HML near the MAT locus through FHA-domain mediated interactions with proteins near the DSB; FKH1 has a paralog, FKH2, that arose from the whole genome duplication Gene:FYV12(YOR183W_p)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GET3(YDL100C)|FD-Score:-5.82|P-value:2.95E-9||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GLG1(YKR058W)|FD-Score:4.5|P-value:3.43E-6||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG1 has a paralog, GLG2, that arose from the whole genome duplication Gene:GYP6(YJL044C)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport Gene:HAL1(YPR005C)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p Gene:HST1(YOL068C)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:HUR1(YGL168W)|FD-Score:-3.17|P-value:7.63E-4||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:IZH2(YOL002C)|FD-Score:4.48|P-value:3.69E-6||SGD DESC:Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc Gene:LIN1(YHR156C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication Gene:MAK3(YPR051W)|FD-Score:-3.26|P-value:5.56E-4||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:MDS3(YGL197W)|FD-Score:3.74|P-value:9.16E-5||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MKT1(YNL085W)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MNN2(YBR015C)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRM2(YGL136C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Mitochondrial 2' O-ribose methyltransferase, required for methylation of U(2791) in 21S rRNA; MRM2 deletion confers thermosensitive respiration and loss of mitochondrial DNA; has similarity to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ Gene:MTC2(YKL098W)|FD-Score:5.18|P-value:1.11E-7||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:NPT1(YOR209C)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:OTU2(YHL013C)|FD-Score:3.12|P-value:8.89E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress Gene:PAN3(YKL025C)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PEX28(YHR150W)|FD-Score:-3.26|P-value:5.50E-4||SGD DESC:Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p Gene:PKR1(YMR123W)|FD-Score:-3.31|P-value:4.71E-4||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PPM1(YDR435C)|FD-Score:-3.64|P-value:1.34E-4||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PTK2(YJR059W)|FD-Score:-3.27|P-value:5.31E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PUS7(YOR243C)|FD-Score:5.02|P-value:2.54E-7||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:QRI5(YLR204W)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:REG1(YDR028C)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:REH1(YLR387C)|FD-Score:7.66|P-value:9.03E-15||SGD DESC:Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains Gene:RIF2(YLR453C)|FD-Score:4.66|P-value:1.57E-6||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RMD1(YDL001W)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Cytoplasmic protein required for sporulation Gene:RPL35B(YDL136W)|FD-Score:-5.31|P-value:5.54E-8||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RSR1(YGR152C)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases Gene:RTT103(YDR289C)|FD-Score:-3.1|P-value:9.66E-4||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:RXT2(YBR095C)|FD-Score:-3.54|P-value:2.03E-4||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SDP1(YIL113W)|FD-Score:-3.28|P-value:5.20E-4||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SGE1(YPR198W)|FD-Score:-5.51|P-value:1.78E-8||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SIN3(YOL004W)|FD-Score:-3.36|P-value:3.87E-4||SGD DESC:Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity Gene:SIP5(YMR140W)|FD-Score:3.13|P-value:8.70E-4||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:SKY1(YMR216C)|FD-Score:-3.4|P-value:3.42E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SLH1(YGR271W)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SLP1(YOR154W_p)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p Gene:SNQ2(YDR011W)|FD-Score:3.88|P-value:5.24E-5||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SPT2(YER161C)|FD-Score:5.63|P-value:8.81E-9||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SPT4(YGR063C)|FD-Score:-3.8|P-value:7.20E-5||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SPT7(YBR081C)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:SSF1(YHR066W)|FD-Score:-3.33|P-value:4.36E-4||SGD DESC:Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication Gene:STP4(YDL048C)|FD-Score:-3.94|P-value:4.02E-5||SGD DESC:Protein containing a Kruppel-type zinc-finger domain; has similarity to Stp1p, Stp2p, and Stp3p; predicted to be a transcription factor; relative distribution to the nucleus increases upon DNA replication stress Gene:SUV3(YPL029W)|FD-Score:-5.28|P-value:6.37E-8||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TPA1(YER049W)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:Poly(rA)-binding protein involved in translation termination efficiency, mRNA poly(A) tail length and mRNA stability; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to prolyl 4-hydroxylases; binds Fe(III) and 2-oxoglutarate Gene:TRP1(YDR007W)|FD-Score:-3.36|P-value:3.92E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:UBP13(YBL067C)|FD-Score:-3.39|P-value:3.52E-4||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:UBP6(YFR010W)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:VMA1(YDL185W)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA9(YCL005W-A)|FD-Score:-3.27|P-value:5.36E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:YAL042C-A(YAL042C-A_d)|FD-Score:4.02|P-value:2.94E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Gene:YCR085W(YCR085W_d)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR034W-B(YDR034W-B_p)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR290W(YDR290W_d)|FD-Score:-4.09|P-value:2.14E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YFL012W(YFL012W_p)|FD-Score:-3.35|P-value:3.99E-4||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YGL214W(YGL214W_d)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C Gene:YGR111W(YGR111W_p)|FD-Score:-4.07|P-value:2.32E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YGR273C(YGR273C_p)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YHL008C(YHL008C_p)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YJL193W(YJL193W_p)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect Gene:YOL099C(YOL099C_d)|FD-Score:3.11|P-value:9.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes Gene:YPK1(YKL126W)|FD-Score:-5.36|P-value:4.27E-8||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR147C(YPR147C_p)|FD-Score:3.83|P-value:6.28E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR204W3.739.45E-50.38DED1ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility
YHR107C3.354.03E-40.24CDC12Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YBR055C3.119.37E-40.02PRP6Splicing factor, component of the U4/U6-U5 snRNP complex
YGR048W3.090.001010.01UFD1Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP)
YGR265W_d3.080.001040.33YGR265W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase
YNR035C2.750.003000.03ARC35Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin
YOR102W_d2.710.003330.02YOR102W_dDubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex
YPR162C2.690.003550.02ORC4Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
YIL046W2.670.003800.02MET30F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus
YBR155W2.650.004030.00CNS1TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion
YOR174W2.650.004040.02MED4Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YGR047C2.630.004250.16TFC4One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102
YBL084C2.470.006810.00CDC27Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YKR062W2.470.006830.08TFA2TFIIE small subunit, involved in RNA polymerase II transcription initiation
YER018C2.390.008500.01SPC25Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR387C7.669.03E-15REH1Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains
YER161C5.638.81E-9SPT2Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins
YAR002C-A5.383.67E-8ERP1Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication
YKL098W5.181.11E-7MTC2Protein of unknown function; mtc2 is synthetically sick with cdc13-1
YOR243C5.022.54E-7PUS7Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria
YLR453C4.661.57E-6RIF2Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation
YKR058W4.503.43E-6GLG1Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG1 has a paralog, GLG2, that arose from the whole genome duplication
YOL002C4.483.69E-6IZH2Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc
YCR085W_d4.261.04E-5YCR085W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YLR172C4.241.12E-5DPH5Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm
YDL001W4.042.70E-5RMD1Cytoplasmic protein required for sporulation
YAL042C-A_d4.022.94E-5YAL042C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene
YER177W4.013.06E-5BMH114-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress
YDR011W3.885.24E-5SNQ2Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species
YPR147C_p3.836.28E-5YPR147C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS

GO enrichment analysis for SGTC_3339
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1562.34E-33SGTC_33439148379 19.0 μMChembridge (Drug-like library)19550360.53125
0.1547.90E-33SGTC_2803448-3607 3.0 μMChemDiv (Drug-like library)6261790.093023360S ribosome export
0.1372.73E-26SGTC_14063909-7906 11.9 μMChemDiv (Drug-like library)X14060.13580260S ribosome export
0.1374.58E-26SGTC_490phenamil 164.0 μMMiscellaneous47550.0987654heme requiring
0.1201.91E-20SGTC_33299141233 27.6 μMChembridge (Drug-like library)49091010.609375
0.1164.87E-19SGTC_33459148410 3.6 μMChembridge (Drug-like library)49035960.767857
0.1132.34E-18SGTC_12690831-0796 3.0 μMChemDiv (Drug-like library)9277580.11392460S ribosome export
0.1133.17E-18SGTC_11433454-2408 5.1 μMChemDiv (Drug-like library)X11430.22560S ribosome export
0.1111.08E-17SGTC_2739tacrine 100.9 μMMiscellaneous19350.112676heme requiring
0.1102.81E-17SGTC_14053909-7894 12.0 μMChemDiv (Drug-like library)71040600.13924160S ribosome export
0.1094.70E-17SGTC_14043909-7820 48.3 μMChemDiv (Drug-like library)237413730.1325360S ribosome export
0.1025.40E-15SGTC_344k064-0035 6.4 μMChemDiv (Drug-like library)50887500.10958960S ribosome export
0.0961.11E-13SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.20779260S ribosome export
0.0946.08E-13SGTC_20422-0095 29.0 μMChemDiv (Drug-like library)67908400.0921053redox potentiating
0.0944.08E-13SGTC_1522st012842 45.6 μMTimTec (Pure natural product library)34910670.134021RNA pol III & RNase P/MRP

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_334591484103.56 μM0.7678574903596Chembridge (Drug-like library)413.514783.9715
SGTC_3333914182012.09 μM0.7454551939433Chembridge (Drug-like library)349.472583.97404
SGTC_3326914006416.38 μM0.7166671868434Chembridge (Drug-like library)379.498563.42815
SGTC_3329914123327.56 μM0.6093754909101Chembridge (Drug-like library)405.416793.39418
SGTC_3319913845016.76 μM0.5671642029690Chembridge (Drug-like library)379.498563.4915
SGTC_3343914837918.98 μM0.531251955036Chembridge (Drug-like library)349.472583.96804
SGTC_334891494643.54 μM0.4852941951442Chembridge (Drug-like library)379.498563.56915
SGTC_3340914663411.1 μM0.4782614907387Chembridge (Drug-like library)365.471983.14615
SGTC_3328914062429.04 μM0.4603174904392Chembridge (Drug-like library)335.4463.33104
SGTC_3349914970323 μM0.4411764910494Chembridge (Drug-like library)351.44542.50915