9146634

2-[4-(3-phenyl-5-propylpyrazolo[1,5-a]pyrimidin-7-yl)piperazin-1-yl]ethanol

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3340
Screen concentration 11.1 μM
Source Chembridge (Drug-like library)
PubChem CID 4907387
SMILES CCCC1=NC2=C(C=NN2C(=C1)N3CCN(CC3)CCO)C4=CC=CC=C4
Standardized SMILES CCCc1cc(N2CCN(CCO)CC2)n3ncc(c4ccccc4)c3n1
Molecular weight 365.472
ALogP 3.15
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.17
% growth inhibition (Hom. pool) 7.78


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4907387
Download HIP data (tab-delimited text)  (excel)
Gene:CCT3(YJL014W)|FD-Score:-3.47|P-value:2.63E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:GPI19(YDR437W)|FD-Score:3.34|P-value:4.13E-4|Clearance:0.16||SGD DESC:Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P Gene:IQG1(YPL242C)|FD-Score:5.26|P-value:7.18E-8|Clearance:1.35||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:IRA1(YBR140C)|FD-Score:-3.59|P-value:1.68E-4|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LSM3(YLR438C-A)|FD-Score:3.11|P-value:9.34E-4|Clearance:0.01||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:PKC1(YBL105C)|FD-Score:-5.67|P-value:7.28E-9|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RPC37(YKR025W)|FD-Score:5.06|P-value:2.14E-7|Clearance:1.35||SGD DESC:RNA polymerase III subunit C37 Gene:RPL33A(YPL143W)|FD-Score:-3.12|P-value:9.16E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:RPS15(YOL040C)|FD-Score:-3.48|P-value:2.48E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RSC4(YKR008W)|FD-Score:-3.46|P-value:2.70E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:SSC1(YJR045C)|FD-Score:3.71|P-value:1.06E-4|Clearance:0.17||SGD DESC:Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication Gene:TBF1(YPL128C)|FD-Score:3.54|P-value:2.01E-4|Clearance:0.14||SGD DESC:Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation Gene:UBC9(YDL064W)|FD-Score:3.4|P-value:3.35E-4|Clearance:0.06||SGD DESC:SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC) Gene:WRS1(YOL097C)|FD-Score:3.19|P-value:7.22E-4|Clearance:0.08||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YJL032W(YJL032W_d)|FD-Score:3.1|P-value:9.53E-4|Clearance:0.2||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase Gene:CCT3(YJL014W)|FD-Score:-3.47|P-value:2.63E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:GPI19(YDR437W)|FD-Score:3.34|P-value:4.13E-4|Clearance:0.16||SGD DESC:Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P Gene:IQG1(YPL242C)|FD-Score:5.26|P-value:7.18E-8|Clearance:1.35||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:IRA1(YBR140C)|FD-Score:-3.59|P-value:1.68E-4|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LSM3(YLR438C-A)|FD-Score:3.11|P-value:9.34E-4|Clearance:0.01||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:PKC1(YBL105C)|FD-Score:-5.67|P-value:7.28E-9|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RPC37(YKR025W)|FD-Score:5.06|P-value:2.14E-7|Clearance:1.35||SGD DESC:RNA polymerase III subunit C37 Gene:RPL33A(YPL143W)|FD-Score:-3.12|P-value:9.16E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:RPS15(YOL040C)|FD-Score:-3.48|P-value:2.48E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RSC4(YKR008W)|FD-Score:-3.46|P-value:2.70E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:SSC1(YJR045C)|FD-Score:3.71|P-value:1.06E-4|Clearance:0.17||SGD DESC:Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication Gene:TBF1(YPL128C)|FD-Score:3.54|P-value:2.01E-4|Clearance:0.14||SGD DESC:Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation Gene:UBC9(YDL064W)|FD-Score:3.4|P-value:3.35E-4|Clearance:0.06||SGD DESC:SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC) Gene:WRS1(YOL097C)|FD-Score:3.19|P-value:7.22E-4|Clearance:0.08||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YJL032W(YJL032W_d)|FD-Score:3.1|P-value:9.53E-4|Clearance:0.2||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4907387
Download HOP data (tab-delimited text)  (excel)
Gene:ARC1(YGL105W)|FD-Score:-3.19|P-value:7.00E-4||SGD DESC:Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress Gene:ARG1(YOL058W)|FD-Score:3.94|P-value:4.14E-5||SGD DESC:Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate Gene:ARO8(YGL202W)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Aromatic aminotransferase I, expression is regulated by general control of amino acid biosynthesis Gene:BNA5(YLR231C)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Kynureninase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BTS1(YPL069C)|FD-Score:-4.1|P-value:2.06E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:DAP2(YHR028C)|FD-Score:4.3|P-value:8.66E-6||SGD DESC:Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p Gene:DBP7(YKR024C)|FD-Score:-3.79|P-value:7.54E-5||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DCC1(YCL016C)|FD-Score:5.43|P-value:2.78E-8||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:DCS1(YLR270W)|FD-Score:-3.44|P-value:2.91E-4||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DDR2(YOL052C-A)|FD-Score:3.9|P-value:4.74E-5||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:EDS1(YBR033W_p)|FD-Score:6.42|P-value:6.67E-11||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:EUG1(YDR518W)|FD-Score:-3.16|P-value:7.96E-4||SGD DESC:Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER Gene:FMP23(YBR047W_p)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRE1(YLR214W)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low copper and iron levels Gene:GCY1(YOR120W)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GDH3(YAL062W)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication Gene:GDT1(YBR187W_p)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GZF3(YJL110C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HIS1(YER055C)|FD-Score:3.32|P-value:4.42E-4||SGD DESC:ATP phosphoribosyltransferase, a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:HSC82(YMR186W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Cytoplasmic chaperone of the Hsp90 family; redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock; contains two acid-rich unstructured regions that promote the solubility of chaperone-substrate complexes; HSC82 has a paralog, HSP82, that arose from the whole genome duplication Gene:HSP26(YBR072W)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity Gene:HUA1(YGR268C)|FD-Score:4.03|P-value:2.77E-5||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:HXT14(YNL318C)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Protein with similarity to hexose transporter family members, expression is induced in low glucose and repressed in high glucose; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ISC1(YER019W)|FD-Score:3.12|P-value:9.13E-4||SGD DESC:Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance Gene:KGD2(YDR148C)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated Gene:KIN4(YOR233W)|FD-Score:5.07|P-value:2.01E-7||SGD DESC:Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication Gene:LTP1(YPR073C)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine Gene:MCK1(YNL307C)|FD-Score:3.78|P-value:7.74E-5||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MNN2(YBR015C)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MTC3(YGL226W)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:MTO1(YGL236C)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants Gene:PIP2(YOR363C)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication Gene:RPS6A(YPL090C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:RRT13(YER066W_p)|FD-Score:4.08|P-value:2.23E-5||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription Gene:SAM37(YMR060C)|FD-Score:-4.53|P-value:2.99E-6||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SAP1(YER047C)|FD-Score:5.45|P-value:2.55E-8||SGD DESC:Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system Gene:SAT4(YCR008W)|FD-Score:3.89|P-value:4.94E-5||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SDH1(YKL148C)|FD-Score:-3.35|P-value:4.03E-4||SGD DESC:Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits Gene:SFH5(YJL145W)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Non-classical phosphatidylinositol transfer protein (PITP); exhibits PI- but not PC-transfer activity; localizes to the peripheral endoplasmic reticulum, cytosol and microsomes; similar to Sec14p; partially relocalizes to the plasma membrane upon DNA replication stress Gene:SHH3(YMR118C_p)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Putative mitochondrial inner membrane protein of unknown function; although similar to Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase Gene:SOL2(YCR073W-A)|FD-Score:5.09|P-value:1.82E-7||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SPO19(YPL130W)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Meiosis-specific prospore protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation; identified as a weak high-copy suppressor of the spo1-1 ts mutation Gene:SSA2(YLL024C)|FD-Score:3.98|P-value:3.49E-5||SGD DESC:ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with paralog Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes Gene:STP3(YLR375W)|FD-Score:-3.99|P-value:3.29E-5||SGD DESC:Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids Gene:TLG2(YOL018C)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:VMA13(YPR036W)|FD-Score:-3.34|P-value:4.16E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS27(YNR006W)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS3(YDR495C)|FD-Score:-3.79|P-value:7.46E-5||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:YAR029W(YAR029W_p)|FD-Score:-4|P-value:3.22E-5||SGD DESC:Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YBL039W-B(YBL039W-B_p)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Putative protein of unknown function Gene:YBR134W(YBR134W_d)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YCR100C(YCR100C_p)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Putative protein of unknown function Gene:YDR003W-A(YDR003W-A_p)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YER085C(YER085C_p)|FD-Score:5.11|P-value:1.60E-7||SGD DESC:Putative protein of unknown function Gene:YGR053C(YGR053C_p)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Putative protein of unknown function Gene:YIL001W(YIL001W_p)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YJL215C(YJL215C_d)|FD-Score:4.6|P-value:2.08E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR020W(YJR020W_d)|FD-Score:-3.75|P-value:8.76E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR434C(YLR434C_d)|FD-Score:3.91|P-value:4.70E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YMR265C(YMR265C_p)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Putative protein of unknown function Gene:YNL122C(YNL122C_p)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YOL099C(YOL099C_d)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes Gene:ARC1(YGL105W)|FD-Score:-3.19|P-value:7.00E-4||SGD DESC:Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress Gene:ARG1(YOL058W)|FD-Score:3.94|P-value:4.14E-5||SGD DESC:Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate Gene:ARO8(YGL202W)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Aromatic aminotransferase I, expression is regulated by general control of amino acid biosynthesis Gene:BNA5(YLR231C)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Kynureninase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BTS1(YPL069C)|FD-Score:-4.1|P-value:2.06E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:DAP2(YHR028C)|FD-Score:4.3|P-value:8.66E-6||SGD DESC:Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p Gene:DBP7(YKR024C)|FD-Score:-3.79|P-value:7.54E-5||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DCC1(YCL016C)|FD-Score:5.43|P-value:2.78E-8||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:DCS1(YLR270W)|FD-Score:-3.44|P-value:2.91E-4||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DDR2(YOL052C-A)|FD-Score:3.9|P-value:4.74E-5||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:EDS1(YBR033W_p)|FD-Score:6.42|P-value:6.67E-11||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:EUG1(YDR518W)|FD-Score:-3.16|P-value:7.96E-4||SGD DESC:Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER Gene:FMP23(YBR047W_p)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRE1(YLR214W)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low copper and iron levels Gene:GCY1(YOR120W)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GDH3(YAL062W)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication Gene:GDT1(YBR187W_p)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GZF3(YJL110C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HIS1(YER055C)|FD-Score:3.32|P-value:4.42E-4||SGD DESC:ATP phosphoribosyltransferase, a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:HSC82(YMR186W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Cytoplasmic chaperone of the Hsp90 family; redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock; contains two acid-rich unstructured regions that promote the solubility of chaperone-substrate complexes; HSC82 has a paralog, HSP82, that arose from the whole genome duplication Gene:HSP26(YBR072W)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity Gene:HUA1(YGR268C)|FD-Score:4.03|P-value:2.77E-5||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:HXT14(YNL318C)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Protein with similarity to hexose transporter family members, expression is induced in low glucose and repressed in high glucose; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ISC1(YER019W)|FD-Score:3.12|P-value:9.13E-4||SGD DESC:Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance Gene:KGD2(YDR148C)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated Gene:KIN4(YOR233W)|FD-Score:5.07|P-value:2.01E-7||SGD DESC:Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication Gene:LTP1(YPR073C)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine Gene:MCK1(YNL307C)|FD-Score:3.78|P-value:7.74E-5||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MNN2(YBR015C)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MTC3(YGL226W)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:MTO1(YGL236C)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants Gene:PIP2(YOR363C)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication Gene:RPS6A(YPL090C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:RRT13(YER066W_p)|FD-Score:4.08|P-value:2.23E-5||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription Gene:SAM37(YMR060C)|FD-Score:-4.53|P-value:2.99E-6||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SAP1(YER047C)|FD-Score:5.45|P-value:2.55E-8||SGD DESC:Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system Gene:SAT4(YCR008W)|FD-Score:3.89|P-value:4.94E-5||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SDH1(YKL148C)|FD-Score:-3.35|P-value:4.03E-4||SGD DESC:Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits Gene:SFH5(YJL145W)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Non-classical phosphatidylinositol transfer protein (PITP); exhibits PI- but not PC-transfer activity; localizes to the peripheral endoplasmic reticulum, cytosol and microsomes; similar to Sec14p; partially relocalizes to the plasma membrane upon DNA replication stress Gene:SHH3(YMR118C_p)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Putative mitochondrial inner membrane protein of unknown function; although similar to Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase Gene:SOL2(YCR073W-A)|FD-Score:5.09|P-value:1.82E-7||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SPO19(YPL130W)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Meiosis-specific prospore protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation; identified as a weak high-copy suppressor of the spo1-1 ts mutation Gene:SSA2(YLL024C)|FD-Score:3.98|P-value:3.49E-5||SGD DESC:ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with paralog Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes Gene:STP3(YLR375W)|FD-Score:-3.99|P-value:3.29E-5||SGD DESC:Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids Gene:TLG2(YOL018C)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:VMA13(YPR036W)|FD-Score:-3.34|P-value:4.16E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS27(YNR006W)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS3(YDR495C)|FD-Score:-3.79|P-value:7.46E-5||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:YAR029W(YAR029W_p)|FD-Score:-4|P-value:3.22E-5||SGD DESC:Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YBL039W-B(YBL039W-B_p)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Putative protein of unknown function Gene:YBR134W(YBR134W_d)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YCR100C(YCR100C_p)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Putative protein of unknown function Gene:YDR003W-A(YDR003W-A_p)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YER085C(YER085C_p)|FD-Score:5.11|P-value:1.60E-7||SGD DESC:Putative protein of unknown function Gene:YGR053C(YGR053C_p)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Putative protein of unknown function Gene:YIL001W(YIL001W_p)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YJL215C(YJL215C_d)|FD-Score:4.6|P-value:2.08E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR020W(YJR020W_d)|FD-Score:-3.75|P-value:8.76E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR434C(YLR434C_d)|FD-Score:3.91|P-value:4.70E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YMR265C(YMR265C_p)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Putative protein of unknown function Gene:YNL122C(YNL122C_p)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YOL099C(YOL099C_d)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL242C5.267.18E-81.35IQG1Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress
YKR025W5.062.14E-71.35RPC37RNA polymerase III subunit C37
YJR045C3.711.06E-40.17SSC1Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication
YPL128C3.542.01E-40.14TBF1Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation
YDL064W3.403.35E-40.06UBC9SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC)
YDR437W3.344.13E-40.16GPI19Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P
YOL097C3.197.22E-40.08WRS1Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA
YLR438C-A3.119.34E-40.01LSM3Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YJL032W_d3.109.53E-40.20YJL032W_dDubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase
YMR296C2.900.001840.04LCB1Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
YGR195W2.860.002090.04SKI6Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
YML130C2.830.002350.02ERO1Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds
YBR087W2.810.002490.02RFC5Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YDR531W2.790.002620.24CAB1Pantothenate kinase (ATP:D-pantothenate 4'-phosphotransferase, EC 2.7.1.33); catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element
YLR355C2.550.005360.02ILV5Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR033W_p6.426.67E-11EDS1_pPutative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication
YER047C5.452.55E-8SAP1Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system
YCL016C5.432.78E-8DCC1Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance
YER085C_p5.111.60E-7YER085C_pPutative protein of unknown function
YCR073W-A5.091.82E-7SOL2Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication
YOR233W5.072.01E-7KIN4Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication
YJL215C_d4.602.08E-6YJL215C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YHR028C4.308.66E-6DAP2Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p
YGL236C4.102.06E-5MTO1Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants
YER066W_p4.082.23E-5RRT13_pPutative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription
YOR363C4.062.47E-5PIP2Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication
YGR268C4.032.77E-5HUA1Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly
YLL024C3.983.49E-5SSA2ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with paralog Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes
YOL058W3.944.14E-5ARG1Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate
YLR434C_d3.914.70E-5YLR434C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W

GO enrichment analysis for SGTC_3340
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0791.37E-9SGTC_477mead ethanolamide 10.0 μMICCB bioactive library160611850.0897436
0.0731.75E-8SGTC_11433454-2408 5.1 μMChemDiv (Drug-like library)X11430.20987760S ribosome export
0.0714.05E-8SGTC_22226885208 172.1 μMChembridge (Fragment library)6095140.0933333
0.0682.01E-7SGTC_615k087-0205 167.0 μMChemDiv (Drug-like library)96427730.0521739
0.0663.27E-7SGTC_33399145883 14.9 μMChembridge (Drug-like library)20306700.478261
0.0631.23E-6SGTC_22436634756 200.0 μMChembridge (Fragment library)8856760.0789474
0.0621.55E-6SGTC_23097664088 163.9 μMChembridge (Fragment library)7049220.0675676
0.0621.82E-6SGTC_14053909-7894 12.0 μMChemDiv (Drug-like library)71040600.12560S ribosome export
0.0612.78E-6SGTC_1887st057648 70.8 μMTimTec (Natural product derivative library)6886720.115385
0.0603.89E-6SGTC_484loperamide 105.0 μMMiscellaneous39550.178571
0.0552.15E-5SGTC_33439148379 19.0 μMChembridge (Drug-like library)19550360.507692
0.0543.06E-5SGTC_33199138450 16.8 μMChembridge (Drug-like library)20296900.26506
0.0534.49E-5SGTC_30399090270 49.5 μMChembridge (Drug-like library)171737500.0731707RPP1 & pyrimidine depletion
0.0525.58E-5SGTC_2739tacrine 100.9 μMMiscellaneous19350.112676heme requiring
0.0526.11E-5SGTC_28769059903 26.0 μMChembridge (Drug-like library)69160950.111111

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_334891494643.54 μM0.7118641951442Chembridge (Drug-like library)379.498563.56915
SGTC_3326914006416.38 μM0.6885251868434Chembridge (Drug-like library)379.498563.42815
SGTC_3349914970323 μM0.6333334910494Chembridge (Drug-like library)351.44542.50915
SGTC_3343914837918.98 μM0.5076921955036Chembridge (Drug-like library)349.472583.96804
SGTC_334591484103.56 μM0.54903596Chembridge (Drug-like library)413.514783.9715
SGTC_3320913700535.14 μM0.4843754908713Chembridge (Drug-like library)335.4463.39704endomembrane recycling
SGTC_3339914588314.94 μM0.4782612030670Chembridge (Drug-like library)379.498563.43515
SGTC_3266913686049.47 μM0.4776124871271Chembridge (Drug-like library)343.424944.02214cell wall
SGTC_3329914123327.56 μM0.4714294909101Chembridge (Drug-like library)405.416793.39418
SGTC_332291390582.11 μM0.4637684908926Chembridge (Drug-like library)343.424943.80714cell wall