9148379

2-methyl-7-(4-methylpiperazin-1-yl)-3-phenyl-5-propylpyrazolo[1,5-a]pyrimidine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3343
Screen concentration 19.0 μM
Source Chembridge (Drug-like library)
PubChem CID 1955036
SMILES CCCC1=NC2=C(C(=NN2C(=C1)N3CCN(CC3)C)C)C4=CC=CC=C4
Standardized SMILES CCCc1cc(N2CCN(C)CC2)n3nc(C)c(c4ccccc4)c3n1
Molecular weight 349.4726
ALogP 3.97
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 19.74
% growth inhibition (Hom. pool) 9.41


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1955036
Download HIP data (tab-delimited text)  (excel)
Gene:AFG2(YLR397C)|FD-Score:3.14|P-value:8.52E-4|Clearance:0.31||SGD DESC:ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs Gene:CCT7(YJL111W)|FD-Score:-3.56|P-value:1.86E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:COP1(YDL145C)|FD-Score:3.62|P-value:1.47E-4|Clearance:0.31||SGD DESC:Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway Gene:MCM10(YIL150C)|FD-Score:3.31|P-value:4.68E-4|Clearance:0.31||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:RPB11(YOL005C)|FD-Score:-3.24|P-value:6.02E-4|Clearance:0||SGD DESC:RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit Gene:RPB8(YOR224C)|FD-Score:-3.25|P-value:5.83E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPL17A(YKL180W)|FD-Score:-4.87|P-value:5.54E-7|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL25(YOL127W)|FD-Score:-4.43|P-value:4.81E-6|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPS15(YOL040C)|FD-Score:-4.14|P-value:1.75E-5|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPS20(YHL015W)|FD-Score:-3.69|P-value:1.13E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:SCD5(YOR329C)|FD-Score:3.39|P-value:3.55E-4|Clearance:0.31||SGD DESC:Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency Gene:TAF12(YDR145W)|FD-Score:-3.1|P-value:9.68E-4|Clearance:0||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TIF34(YMR146C)|FD-Score:-3.33|P-value:4.32E-4|Clearance:0||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:YDL196W(YDL196W_d)|FD-Score:-3.63|P-value:1.44E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YGR115C(YGR115C_d)|FD-Score:3.33|P-value:4.36E-4|Clearance:0.31||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YNL247W(YNL247W)|FD-Score:3.14|P-value:8.41E-4|Clearance:0.31||SGD DESC:Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments Gene:AFG2(YLR397C)|FD-Score:3.14|P-value:8.52E-4|Clearance:0.31||SGD DESC:ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs Gene:CCT7(YJL111W)|FD-Score:-3.56|P-value:1.86E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:COP1(YDL145C)|FD-Score:3.62|P-value:1.47E-4|Clearance:0.31||SGD DESC:Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway Gene:MCM10(YIL150C)|FD-Score:3.31|P-value:4.68E-4|Clearance:0.31||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:RPB11(YOL005C)|FD-Score:-3.24|P-value:6.02E-4|Clearance:0||SGD DESC:RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit Gene:RPB8(YOR224C)|FD-Score:-3.25|P-value:5.83E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPL17A(YKL180W)|FD-Score:-4.87|P-value:5.54E-7|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL25(YOL127W)|FD-Score:-4.43|P-value:4.81E-6|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPS15(YOL040C)|FD-Score:-4.14|P-value:1.75E-5|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPS20(YHL015W)|FD-Score:-3.69|P-value:1.13E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:SCD5(YOR329C)|FD-Score:3.39|P-value:3.55E-4|Clearance:0.31||SGD DESC:Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency Gene:TAF12(YDR145W)|FD-Score:-3.1|P-value:9.68E-4|Clearance:0||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TIF34(YMR146C)|FD-Score:-3.33|P-value:4.32E-4|Clearance:0||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:YDL196W(YDL196W_d)|FD-Score:-3.63|P-value:1.44E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YGR115C(YGR115C_d)|FD-Score:3.33|P-value:4.36E-4|Clearance:0.31||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YNL247W(YNL247W)|FD-Score:3.14|P-value:8.41E-4|Clearance:0.31||SGD DESC:Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1955036
Download HOP data (tab-delimited text)  (excel)
Gene:AIM43(YPL099C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARC1(YGL105W)|FD-Score:-3.95|P-value:3.85E-5||SGD DESC:Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress Gene:BOI2(YER114C)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication Gene:COQ8(YGL119W)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication Gene:CRS5(YOR031W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Copper-binding metallothionein, required for wild-type copper resistance Gene:CWH43(YCR017C)|FD-Score:3.18|P-value:7.24E-4||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:CYT2(YKL087C)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 Gene:MKS1(YNL076W)|FD-Score:3.68|P-value:1.14E-4||SGD DESC:Pleiotropic negative transcriptional regulator involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling Gene:MRP7(YNL005C)|FD-Score:-3.15|P-value:8.05E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSS18(YPR134W)|FD-Score:4|P-value:3.11E-5||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:NHP10(YDL002C)|FD-Score:-3.45|P-value:2.84E-4||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:PAA1(YDR071C)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication Gene:PER33(YLR064W)|FD-Score:4.96|P-value:3.57E-7||SGD DESC:Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress Gene:QDR2(YIL121W)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; exports copper; has broad substrate specificity and can transport many mono- and divalent cations; transports a variety of drugs and is required for resistance to quinidine, barban, cisplatin, and bleomycin; contributes to potassium homeostasis; expression is regulated by copper Gene:REH1(YLR387C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains Gene:RIM101(YHL027W)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:SHE3(YBR130C)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Protein that acts as an adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance Gene:SPE4(YLR146C)|FD-Score:3.81|P-value:6.93E-5||SGD DESC:Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid Gene:SUV3(YPL029W)|FD-Score:-5.5|P-value:1.88E-8||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TLG2(YOL018C)|FD-Score:-3.35|P-value:3.99E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOF2(YKR010C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VPS15(YBR097W)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:YBR225W(YBR225W_p)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components Gene:YDL159W-A(YDL159W-A_p)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species Gene:YDR015C(YDR015C_d)|FD-Score:6.41|P-value:7.19E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A Gene:YIL001W(YIL001W_p)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YIL055C(YIL055C_p)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Putative protein of unknown function Gene:YIL166C(YIL166C_p)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene Gene:YLL054C(YLL054C_p)|FD-Score:-3.85|P-value:5.88E-5||SGD DESC:Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene Gene:YLR042C(YLR042C_p)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene Gene:YNR062C(YNR062C_p)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Putative membrane protein of unknown function Gene:YPL191C(YPL191C_p)|FD-Score:4.74|P-value:1.08E-6||SGD DESC:Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:AIM43(YPL099C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARC1(YGL105W)|FD-Score:-3.95|P-value:3.85E-5||SGD DESC:Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress Gene:BOI2(YER114C)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication Gene:COQ8(YGL119W)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication Gene:CRS5(YOR031W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Copper-binding metallothionein, required for wild-type copper resistance Gene:CWH43(YCR017C)|FD-Score:3.18|P-value:7.24E-4||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:CYT2(YKL087C)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 Gene:MKS1(YNL076W)|FD-Score:3.68|P-value:1.14E-4||SGD DESC:Pleiotropic negative transcriptional regulator involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling Gene:MRP7(YNL005C)|FD-Score:-3.15|P-value:8.05E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSS18(YPR134W)|FD-Score:4|P-value:3.11E-5||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:NHP10(YDL002C)|FD-Score:-3.45|P-value:2.84E-4||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:PAA1(YDR071C)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication Gene:PER33(YLR064W)|FD-Score:4.96|P-value:3.57E-7||SGD DESC:Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress Gene:QDR2(YIL121W)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; exports copper; has broad substrate specificity and can transport many mono- and divalent cations; transports a variety of drugs and is required for resistance to quinidine, barban, cisplatin, and bleomycin; contributes to potassium homeostasis; expression is regulated by copper Gene:REH1(YLR387C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains Gene:RIM101(YHL027W)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:SHE3(YBR130C)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Protein that acts as an adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance Gene:SPE4(YLR146C)|FD-Score:3.81|P-value:6.93E-5||SGD DESC:Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid Gene:SUV3(YPL029W)|FD-Score:-5.5|P-value:1.88E-8||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TLG2(YOL018C)|FD-Score:-3.35|P-value:3.99E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOF2(YKR010C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VPS15(YBR097W)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:YBR225W(YBR225W_p)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components Gene:YDL159W-A(YDL159W-A_p)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species Gene:YDR015C(YDR015C_d)|FD-Score:6.41|P-value:7.19E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A Gene:YIL001W(YIL001W_p)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YIL055C(YIL055C_p)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Putative protein of unknown function Gene:YIL166C(YIL166C_p)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene Gene:YLL054C(YLL054C_p)|FD-Score:-3.85|P-value:5.88E-5||SGD DESC:Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene Gene:YLR042C(YLR042C_p)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene Gene:YNR062C(YNR062C_p)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Putative membrane protein of unknown function Gene:YPL191C(YPL191C_p)|FD-Score:4.74|P-value:1.08E-6||SGD DESC:Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL145C3.621.47E-40.31COP1Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway
YOR329C3.393.55E-40.31SCD5Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency
YGR115C_d3.334.36E-40.31YGR115C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C
YIL150C3.314.68E-40.31MCM10Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins
YNL247W3.148.41E-40.31YNL247WCysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments
YLR397C3.148.52E-40.31AFG2ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs
YOR095C2.830.002320.06RKI1Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis
YJL033W2.770.002810.03HCA4DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis
YLR438C-A2.740.003100.07LSM3Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YGL137W2.670.003830.05SEC27Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP
YDR246W2.620.004450.01TRS23One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4
YER048W-A2.600.004600.01ISD11Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis
YOR057W2.590.004730.07SGT1Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress
YER112W2.520.005790.03LSM4Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress
YBL035C2.490.006360.09POL12B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR015C_d6.417.19E-11YDR015C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A
YLR064W4.963.57E-7PER33Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress
YPL191C_p4.741.08E-6YPL191C_pPutative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YPR134W4.003.11E-5MSS18Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions
YLR146C3.816.93E-5SPE4Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid
YKR010C3.721.01E-4TOF2Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication
YNL076W3.681.14E-4MKS1Pleiotropic negative transcriptional regulator involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling
YLR387C3.681.15E-4REH1Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains
YBR225W_p3.621.50E-4YBR225W_pPutative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components
YPL099C3.591.63E-4AIM43Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
YIL166C_p3.581.74E-4YIL166C_pPutative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene
YOR031W3.472.59E-4CRS5Copper-binding metallothionein, required for wild-type copper resistance
YER114C3.383.65E-4BOI2Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication
YBR097W3.383.69E-4VPS15Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery
YDR071C3.354.02E-4PAA1Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication

GO enrichment analysis for SGTC_3343
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1562.34E-33SGTC_33399145883 14.9 μMChembridge (Drug-like library)20306700.53125
0.1323.22E-24SGTC_490phenamil 164.0 μMMiscellaneous47550.103896heme requiring
0.1218.78E-21SGTC_11433454-2408 5.1 μMChemDiv (Drug-like library)X11430.30555660S ribosome export
0.1001.70E-14SGTC_33459148410 3.6 μMChembridge (Drug-like library)49035960.557377
0.0897.12E-12SGTC_2766oltipraz 83.9 μMMiscellaneous473180.0714286heme requiring
0.0831.84E-10SGTC_1467k284-1082 293.0 μMChemDiv (Drug-like library)33347050.0897436
0.0832.09E-10SGTC_2739tacrine 100.9 μMMiscellaneous19350.119403heme requiring
0.0782.03E-9SGTC_2803448-3607 3.0 μMChemDiv (Drug-like library)6261790.09756160S ribosome export
0.0773.56E-9SGTC_32499135183 49.5 μMChembridge (Drug-like library)49027790.33333360S ribosome export
0.0708.27E-8SGTC_1786berberine 21.6 μMMiscellaneous124560.068965560S ribosome export
0.0699.87E-8SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.13414660S ribosome export
0.0681.85E-7SGTC_1996st072960 66.8 μMTimTec (Natural product derivative library)17194050.101266
0.0672.76E-7SGTC_32229128301 49.5 μMChembridge (Drug-like library)49113580.5560S ribosome export
0.0672.97E-7SGTC_14053909-7894 12.0 μMChemDiv (Drug-like library)71040600.14666760S ribosome export
0.0621.97E-6SGTC_9891501-0895 10.3 μMChemDiv (Drug-like library)28301120.128205

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3326914006416.38 μM0.7678571868434Chembridge (Drug-like library)379.498563.42815
SGTC_3333914182012.09 μM0.6727271939433Chembridge (Drug-like library)349.472583.97404
SGTC_3328914062429.04 μM0.64904392Chembridge (Drug-like library)335.4463.33104
SGTC_334591484103.56 μM0.5573774903596Chembridge (Drug-like library)413.514783.9715
SGTC_3222912830149.47 μM0.554911358Chembridge (Drug-like library)375.390813.9330760S ribosome export
SGTC_3280914096149.47 μM0.5333334901226Chembridge (Drug-like library)355.864483.5090460S ribosome export
SGTC_3339914588314.94 μM0.531252030670Chembridge (Drug-like library)379.498563.43515
SGTC_3340914663411.1 μM0.5076924907387Chembridge (Drug-like library)365.471983.14615
SGTC_335691518114.48 μM0.51859254Chembridge (Drug-like library)357.451524.0914cell wall
SGTC_3329914123327.56 μM0.4347834909101Chembridge (Drug-like library)405.416793.39418