4111-0029

2-phenyl-5-pyridin-3-yl-5,10b-dihydro-1H-pyrazolo[1,5-c][1,3]benzoxazine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_336
Screen concentration 111.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 3131175
SMILES C1C2C3=CC=CC=C3OC(N2N=C1C4=CC=CC=C4)C5=CN=CC=C5
Standardized SMILES C1C2N(N=C1c3ccccc3)C(Oc4ccccc24)c5cccnc5
Molecular weight 327.3792
ALogP 3.53
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.08
% growth inhibition (Hom. pool) 7.23


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3131175
Download HIP data (tab-delimited text)  (excel)
Gene:DBP5(YOR046C)|FD-Score:-3.13|P-value:8.82E-4|Clearance:0||SGD DESC:Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p Gene:FAS2(YPL231W)|FD-Score:-3.16|P-value:7.80E-4|Clearance:0||SGD DESC:Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities Gene:LAS17(YOR181W)|FD-Score:-3.45|P-value:2.78E-4|Clearance:0||SGD DESC:Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) Gene:RAP1(YNL216W)|FD-Score:3.42|P-value:3.16E-4|Clearance:0.53||SGD DESC:Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) Gene:RPC11(YDR045C)|FD-Score:3.73|P-value:9.64E-5|Clearance:0.28||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:RSC9(YML127W)|FD-Score:-3.13|P-value:8.85E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SAM35(YHR083W)|FD-Score:4.53|P-value:2.97E-6|Clearance:0.8||SGD DESC:Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane Gene:TAP42(YMR028W)|FD-Score:-3.4|P-value:3.35E-4|Clearance:0||SGD DESC:Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits Gene:YGR114C(YGR114C_d)|FD-Score:3.45|P-value:2.83E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:DBP5(YOR046C)|FD-Score:-3.13|P-value:8.82E-4|Clearance:0||SGD DESC:Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p Gene:FAS2(YPL231W)|FD-Score:-3.16|P-value:7.80E-4|Clearance:0||SGD DESC:Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities Gene:LAS17(YOR181W)|FD-Score:-3.45|P-value:2.78E-4|Clearance:0||SGD DESC:Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) Gene:RAP1(YNL216W)|FD-Score:3.42|P-value:3.16E-4|Clearance:0.53||SGD DESC:Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) Gene:RPC11(YDR045C)|FD-Score:3.73|P-value:9.64E-5|Clearance:0.28||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:RSC9(YML127W)|FD-Score:-3.13|P-value:8.85E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SAM35(YHR083W)|FD-Score:4.53|P-value:2.97E-6|Clearance:0.8||SGD DESC:Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane Gene:TAP42(YMR028W)|FD-Score:-3.4|P-value:3.35E-4|Clearance:0||SGD DESC:Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits Gene:YGR114C(YGR114C_d)|FD-Score:3.45|P-value:2.83E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3131175
Download HOP data (tab-delimited text)  (excel)
Gene:ENV9(YOR246C)|FD-Score:7.1|P-value:6.06E-13||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:FKS3(YMR306W)|FD-Score:4.33|P-value:7.40E-6||SGD DESC:Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMT1(YBL013W)|FD-Score:5.12|P-value:1.49E-7||SGD DESC:Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate Gene:GLG2(YJL137C)|FD-Score:-4|P-value:3.17E-5||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication Gene:GTR1(YML121W)|FD-Score:-3.32|P-value:4.56E-4||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:HBT1(YDL223C)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:MHO1(YJR008W)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Protein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility Gene:MSH3(YCR092C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:PEX13(YLR191W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p Gene:PGD1(YGL025C)|FD-Score:4.33|P-value:7.48E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PKH3(YDR466W)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PPR1(YLR014C)|FD-Score:4.6|P-value:2.13E-6||SGD DESC:Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p Gene:PRY1(YJL079C)|FD-Score:-3.1|P-value:9.53E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication Gene:RHB1(YCR027C)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins Gene:SAP4(YGL229C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SEC66(YBR171W)|FD-Score:4.48|P-value:3.77E-6||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:STI1(YOR027W)|FD-Score:-3.25|P-value:5.87E-4||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:TGL3(YMR313C)|FD-Score:5.93|P-value:1.52E-9||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation Gene:TMA108(YIL137C)|FD-Score:3.14|P-value:8.54E-4||SGD DESC:Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease Gene:VHS3(YOR054C)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:YBR090C(YBR090C_p)|FD-Score:-3.52|P-value:2.14E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YDR306C(YDR306C_p)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YGL041C-B(YGL041C-B_p)|FD-Score:4.81|P-value:7.51E-7||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGR111W(YGR111W_p)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YJL119C(YJL119C_d)|FD-Score:-3.45|P-value:2.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML082W(YML082W_p)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene Gene:YOL085C(YOL085C_d)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOL085W-A Gene:YPL216W(YPL216W_p)|FD-Score:3.77|P-value:8.25E-5||SGD DESC:Putative protein of unknown function; YPL216W is not an essential gene Gene:YVC1(YOR087W)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock Gene:ENV9(YOR246C)|FD-Score:7.1|P-value:6.06E-13||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:FKS3(YMR306W)|FD-Score:4.33|P-value:7.40E-6||SGD DESC:Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMT1(YBL013W)|FD-Score:5.12|P-value:1.49E-7||SGD DESC:Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate Gene:GLG2(YJL137C)|FD-Score:-4|P-value:3.17E-5||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication Gene:GTR1(YML121W)|FD-Score:-3.32|P-value:4.56E-4||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:HBT1(YDL223C)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:MHO1(YJR008W)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Protein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility Gene:MSH3(YCR092C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:PEX13(YLR191W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p Gene:PGD1(YGL025C)|FD-Score:4.33|P-value:7.48E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PKH3(YDR466W)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PPR1(YLR014C)|FD-Score:4.6|P-value:2.13E-6||SGD DESC:Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p Gene:PRY1(YJL079C)|FD-Score:-3.1|P-value:9.53E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication Gene:RHB1(YCR027C)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins Gene:SAP4(YGL229C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SEC66(YBR171W)|FD-Score:4.48|P-value:3.77E-6||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:STI1(YOR027W)|FD-Score:-3.25|P-value:5.87E-4||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:TGL3(YMR313C)|FD-Score:5.93|P-value:1.52E-9||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation Gene:TMA108(YIL137C)|FD-Score:3.14|P-value:8.54E-4||SGD DESC:Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease Gene:VHS3(YOR054C)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:YBR090C(YBR090C_p)|FD-Score:-3.52|P-value:2.14E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YDR306C(YDR306C_p)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YGL041C-B(YGL041C-B_p)|FD-Score:4.81|P-value:7.51E-7||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGR111W(YGR111W_p)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YJL119C(YJL119C_d)|FD-Score:-3.45|P-value:2.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML082W(YML082W_p)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene Gene:YOL085C(YOL085C_d)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOL085W-A Gene:YPL216W(YPL216W_p)|FD-Score:3.77|P-value:8.25E-5||SGD DESC:Putative protein of unknown function; YPL216W is not an essential gene Gene:YVC1(YOR087W)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR083W4.532.97E-60.80SAM35Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane
YDR045C3.739.64E-50.28RPC11RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS
YGR114C_d3.452.83E-40.03YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YNL216W3.423.16E-40.53RAP1Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain)
YBR155W2.890.001950.05CNS1TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion
YER038C2.840.002270.02KRE29Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
YPR094W2.820.002440.12RDS3Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance
YLR060W2.700.003490.02FRS1Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control
YLL036C2.680.003690.06PRP19Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain
YLR086W2.620.004380.01SMC4Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate
YOR168W2.610.004460.09GLN4Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions
YKL013C2.520.005810.02ARC19Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YMR049C2.510.006090.05ERB1Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1
YMR013C2.450.007060.02SEC59Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation
YLR132C2.430.007540.07USB1Putative phosphodiesterase specific for U6 snRNA 3' end modification; trims the 3' poly(u) tract to leave a terminal 3' phosphate; human homolog, hUSB1 (aka C16orf57) produces a 2',3' cyclic phosphate; mutations in hUSB1 are associated with a rare skin condition (OMIM 604173); essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR246C7.106.06E-13ENV9Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
YMR313C5.931.52E-9TGL3Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation
YBL013W5.121.49E-7FMT1Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate
YGL041C-B_p4.817.51E-7YGL041C-B_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YLR014C4.602.13E-6PPR1Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p
YBR171W4.483.77E-6SEC66Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YMR306W4.337.40E-6FKS3Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YGL025C4.337.48E-6PGD1Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
YPL216W_p3.778.25E-5YPL216W_pPutative protein of unknown function; YPL216W is not an essential gene
YCR092C3.571.77E-4MSH3Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability
YLR191W3.551.93E-4PEX13Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p
YGL229C3.492.45E-4SAP4Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p
YGR111W_p3.462.74E-4YGR111W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
YOR054C3.462.74E-4VHS3Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis
YDR466W3.423.17E-4PKH3Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant

GO enrichment analysis for SGTC_336
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1404.23E-27SGTC_2094077-0173 175.6 μMChemDiv (Drug-like library)159934940.354839
0.1256.82E-22SGTC_2413inermin 26.1 μMTimTec (Natural product derivative library)915100.126761
0.1241.01E-21SGTC_29679089538 49.9 μMChembridge (Drug-like library)7282160.150685
0.1163.36E-19SGTC_21545570129 200.0 μMChembridge (Fragment library)6733660.142857
0.0815.78E-10SGTC_30909117696 49.5 μMChembridge (Drug-like library)79238210.0666667
0.0808.93E-10SGTC_23859074692 200.0 μMChembridge (Fragment library)170469060.047619
0.0764.18E-9SGTC_32419133421 49.5 μMChembridge (Drug-like library)170182210.076087PDR1
0.0741.11E-8SGTC_11710368-0077 21.4 μMChemDiv (Drug-like library)32397790.164179
0.0731.56E-8SGTC_7251497-0963 19.0 μMChemDiv (Drug-like library)35499770.0394737copper-dependent oxidative stress
0.0731.99E-8SGTC_1000086-0128 49.6 μMChemDiv (Drug-like library)932970.0540541copper-dependent oxidative stress
0.0714.75E-8SGTC_1235benzylparaben 21.5 μMChemDiv (Drug-like library)71800.0909091
0.0714.87E-8SGTC_31729105863 49.5 μMChembridge (Drug-like library)23487850.0833333ERG2
0.0715.18E-8SGTC_8053852-0218 479.0 μMChemDiv (Drug-like library)66153550.0769231ERAD & cell cycle
0.0681.78E-7SGTC_2710st078562 53.7 μMTimTec (Natural product derivative library)172509080.0659341
0.0664.39E-7SGTC_3301488-0319 80.1 μMChemDiv (Drug-like library)68466140.0722892RPP1 & pyrimidine depletion

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1534077-0274519.67 μM0.3714293121976ChemDiv (Drug-like library)384.8543635.49905
SGTC_2094077-0173175.59 μM0.35483915993494ChemDiv (Drug-like library)284.740223.53903
SGTC_1656st01292111 μM0.2903236732821TimTec (Natural product derivative library)375.14928-0.315
SGTC_1658st01294420 μM0.29032324207260TimTec (Natural product derivative library)499.334675-2.601915iron homeostasis
SGTC_1682st012968530 nM0.29032324207270TimTec (Natural product derivative library)376.766681.71635
SGTC_1798st04571960.95 μM0.253980186TimTec (Natural product derivative library)318.350960.70606
SGTC_20765212012200 μM0.245902604802Chembridge (Fragment library)255.298360.31226
SGTC_15465-methoxyflavanone78.7 μM0.230769147795TimTec (Pure natural product library)254.280563.08303
SGTC_1659st01295442.1 μM0.23076924207262TimTec (Natural product derivative library)476.319361.98627
SGTC_1889st0590805.18 μM0.225806176925TimTec (Natural product derivative library)240.253982.85713