1362-0200

1-ethyl-2,3,3-trimethyl-5-phenylindol-1-ium

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_345
Screen concentration 395.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 1419823
SMILES CC[N+]1=C(C(C2=C1C=CC(=C2)C3=CC=CC=C3)(C)C)C
Standardized SMILES CC[N+]1=C(C)C(C)(C)c2cc(ccc12)c3ccccc3
Molecular weight 264.3847
ALogP 4.45
H-bond donor count 0
H-bond acceptor count 0
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.57
% growth inhibition (Hom. pool) 5.44


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1419823
Download HIP data (tab-delimited text)  (excel)
Gene:CDC25(YLR310C)|FD-Score:3.19|P-value:7.22E-4|Clearance:0||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:GLC7(YER133W)|FD-Score:4.48|P-value:3.74E-6|Clearance:0.57||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:KIN28(YDL108W)|FD-Score:4.18|P-value:1.44E-5|Clearance:0.57||SGD DESC:Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters Gene:RIO1(YOR119C)|FD-Score:3.1|P-value:9.56E-4|Clearance:0.19||SGD DESC:Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA Gene:RPN2(YIL075C)|FD-Score:3.61|P-value:1.53E-4|Clearance:0.39||SGD DESC:Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress Gene:RPP1(YHR062C)|FD-Score:-3.18|P-value:7.45E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRN3(YKL125W)|FD-Score:3.22|P-value:6.48E-4|Clearance:0.03||SGD DESC:Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo Gene:SEC59(YMR013C)|FD-Score:3.22|P-value:6.34E-4|Clearance:0.01||SGD DESC:Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation Gene:SPC42(YKL042W)|FD-Score:3.14|P-value:8.50E-4|Clearance:0.03||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:SWC4(YGR002C)|FD-Score:3.18|P-value:7.24E-4|Clearance:0.05||SGD DESC:Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex Gene:YLR076C(YLR076C_d)|FD-Score:4.37|P-value:6.29E-6|Clearance:0.57||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:CDC25(YLR310C)|FD-Score:3.19|P-value:7.22E-4|Clearance:0||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:GLC7(YER133W)|FD-Score:4.48|P-value:3.74E-6|Clearance:0.57||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:KIN28(YDL108W)|FD-Score:4.18|P-value:1.44E-5|Clearance:0.57||SGD DESC:Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters Gene:RIO1(YOR119C)|FD-Score:3.1|P-value:9.56E-4|Clearance:0.19||SGD DESC:Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA Gene:RPN2(YIL075C)|FD-Score:3.61|P-value:1.53E-4|Clearance:0.39||SGD DESC:Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress Gene:RPP1(YHR062C)|FD-Score:-3.18|P-value:7.45E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRN3(YKL125W)|FD-Score:3.22|P-value:6.48E-4|Clearance:0.03||SGD DESC:Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo Gene:SEC59(YMR013C)|FD-Score:3.22|P-value:6.34E-4|Clearance:0.01||SGD DESC:Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation Gene:SPC42(YKL042W)|FD-Score:3.14|P-value:8.50E-4|Clearance:0.03||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:SWC4(YGR002C)|FD-Score:3.18|P-value:7.24E-4|Clearance:0.05||SGD DESC:Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex Gene:YLR076C(YLR076C_d)|FD-Score:4.37|P-value:6.29E-6|Clearance:0.57||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1419823
Download HOP data (tab-delimited text)  (excel)
Gene:ADE8(YDR408C)|FD-Score:-4.97|P-value:3.38E-7||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:AFT1(YGL071W)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ALD5(YER073W)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed Gene:ALD6(YPL061W)|FD-Score:-3.28|P-value:5.17E-4||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ALF1(YNL148C)|FD-Score:-3.21|P-value:6.61E-4||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ANP1(YEL036C)|FD-Score:-3.28|P-value:5.23E-4||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:APE2(YKL157W)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication Gene:ASE1(YOR058C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Mitotic spindle midzone localized protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress Gene:CIT3(YPR001W)|FD-Score:5.73|P-value:4.94E-9||SGD DESC:Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate Gene:COG5(YNL051W)|FD-Score:-4.61|P-value:2.05E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSR1(YLR380W)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress Gene:DCI1(YOR180C)|FD-Score:5.6|P-value:1.07E-8||SGD DESC:Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed Gene:DFG16(YOR030W)|FD-Score:4.56|P-value:2.55E-6||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DIE2(YGR227W)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:ECM22(YLR228C)|FD-Score:7.7|P-value:7.03E-15||SGD DESC:Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication Gene:EMC2(YJR088C)|FD-Score:3.73|P-value:9.66E-5||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35 Gene:FAA2(YER015W)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome Gene:FLO10(YKR102W)|FD-Score:-3.22|P-value:6.32E-4||SGD DESC:Member of the FLO family of cell wall flocculation proteins; not expressed in most lab strains; overproduction induces flocculation that can be inhibited by mannose, sucrose, or glucose; overproduction also promotes haploid invasive growth and diploid filamentous growth Gene:FYV6(YNL133C)|FD-Score:3.13|P-value:8.86E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GAT3(YLR013W)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Protein containing GATA family zinc finger motifs Gene:GDA1(YEL042W)|FD-Score:-4.28|P-value:9.46E-6||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:GMC2(YLR445W)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor Gene:GND1(YHR183W)|FD-Score:-3.95|P-value:3.99E-5||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:HXT10(YFL011W)|FD-Score:3.86|P-value:5.57E-5||SGD DESC:Putative hexose transporter, expressed at low levels and expression is repressed by glucose Gene:IME1(YJR094C)|FD-Score:8.23|P-value:9.71E-17||SGD DESC:Master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p Gene:IMG2(YCR071C)|FD-Score:-3.64|P-value:1.39E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:IRS4(YKR019C)|FD-Score:-6.13|P-value:4.30E-10||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MAP1(YLR244C)|FD-Score:4.27|P-value:9.56E-6||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MBB1(YJL199C_d)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies Gene:MCH5(YOR306C)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport Gene:MRP13(YGR084C)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSP1(YGR028W)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Mitochondrial protein involved in sorting of proteins in the mitochondria; putative membrane-spanning ATPase Gene:NIT3(YLR351C)|FD-Score:-3.19|P-value:7.14E-4||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:OPI3(YJR073C)|FD-Score:3.1|P-value:9.83E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PET111(YMR257C)|FD-Score:-3.2|P-value:6.98E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PET117(YER058W)|FD-Score:3.87|P-value:5.34E-5||SGD DESC:Protein required for assembly of cytochrome c oxidase Gene:PEX21(YGR239C)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Peroxin required for peroxisomal matrix protein targeting; acts on proteins containing the PTS2 targeting sequence; interacts with Pex7p; partially redundant with Pex18p; relative distribution to cytoplasmic foci increases upon DNA replication stress Gene:PHO89(YBR296C)|FD-Score:-3.92|P-value:4.43E-5||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PIR3(YKL163W)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication Gene:PMD1(YER132C)|FD-Score:4.34|P-value:7.29E-6||SGD DESC:Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions Gene:PTC7(YHR076W)|FD-Score:3.92|P-value:4.50E-5||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:RAD17(YOR368W)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RGP1(YDR137W)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIM9(YMR063W)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RKR1(YMR247C)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice Gene:RMT2(YDR465C)|FD-Score:4.03|P-value:2.77E-5||SGD DESC:Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS10A(YOR293W)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication Gene:RRT2(YBR246W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:SAW1(YAL027W)|FD-Score:-3.73|P-value:9.41E-5||SGD DESC:5'- and 3'-flap DNA binding protein; stimulates the endonuclease activity of Rad1/Rad10p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:SFM1(YOR021C)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism Gene:SLC1(YDL052C)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes Gene:SNF6(YHL025W)|FD-Score:-3.25|P-value:5.76E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SWA2(YDR320C)|FD-Score:-4.16|P-value:1.60E-5||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SWT1(YOR166C)|FD-Score:4.11|P-value:2.01E-5||SGD DESC:RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain Gene:THI73(YLR004C)|FD-Score:-3.38|P-value:3.61E-4||SGD DESC:Putative plasma membrane permease proposed to be involved in carboxylic acid uptake and repressed by thiamine; substrate of Dbf2p/Mob1p kinase; transcription is altered if mitochondrial dysfunction occurs Gene:URN1(YPR152C)|FD-Score:-3.61|P-value:1.53E-4||SGD DESC:Putative protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase Gene:VAC8(YEL013W)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:VPS1(YKR001C)|FD-Score:-4.66|P-value:1.62E-6||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:XDJ1(YLR090W)|FD-Score:4.02|P-value:2.96E-5||SGD DESC:Chaperone with a role in facilitating mitochondrial protein import; ascomycete-specific member of the DnaJ-like family, closely related to Ydj1p; predicted to be C-terminally prenylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YAR029W(YAR029W_p)|FD-Score:3.92|P-value:4.43E-5||SGD DESC:Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YCL074W(YCL074W)|FD-Score:-3.19|P-value:7.18E-4||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YDL159W-A(YDL159W-A_p)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species Gene:YDR391C(YDR391C_p)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YER066C-A(YER066C-A_d)|FD-Score:-6.16|P-value:3.67E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, partially overlaps uncharacterized ORF YER067W Gene:YGL159W(YGL159W_p)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YGL188C-A(YGL188C-A_p)|FD-Score:-3.2|P-value:6.90E-4||SGD DESC:Putative protein of unknown function Gene:YGR079W(YGR079W_p)|FD-Score:4.35|P-value:6.92E-6||SGD DESC:Putative protein of unknown function; YGR079W is not an essential gene Gene:YGR122W(YGR122W)|FD-Score:6.35|P-value:1.09E-10||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YHL042W(YHL042W_p)|FD-Score:-3.24|P-value:6.07E-4||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:YJR005C-A(YJR005C-A_p)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Putative protein of unknown function; originally identified as a syntenic homolog of an <i>Ashbya gossypii</i> gene; YJR005C-A has a paralog, YGR169C-A, that arose from the whole genome duplication Gene:YJR142W(YJR142W_p)|FD-Score:3.16|P-value:7.78E-4||SGD DESC:Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member Gene:YLL056C(YLL056C_p)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:Putative protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p and genes involved in pleiotropic drug resistance (PDR); expression is induced in cells treated with the mycotoxin patulin Gene:YML034C-A(YML034C-A_d)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR105W-A(YMR105W-A_p)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Putative protein of unknown function Gene:YMR158C-A(YMR158C-A_p)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Putative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene Gene:YMR172C-A(YMR172C-A_d)|FD-Score:3.89|P-value:5.06E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNG1(YOR064C)|FD-Score:4.59|P-value:2.26E-6||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1 Gene:YNL338W(YNL338W_d)|FD-Score:-4.17|P-value:1.51E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps TEL14L-XC, which is Telomeric X element Core sequence on the left arm of Chromosome XIV Gene:YOR225W(YOR225W_d)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR078C(YPR078C_p)|FD-Score:3.88|P-value:5.25E-5||SGD DESC:Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible Gene:YPS7(YDR349C)|FD-Score:4.33|P-value:7.43E-6||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum Gene:ADE8(YDR408C)|FD-Score:-4.97|P-value:3.38E-7||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:AFT1(YGL071W)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ALD5(YER073W)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed Gene:ALD6(YPL061W)|FD-Score:-3.28|P-value:5.17E-4||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ALF1(YNL148C)|FD-Score:-3.21|P-value:6.61E-4||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ANP1(YEL036C)|FD-Score:-3.28|P-value:5.23E-4||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:APE2(YKL157W)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication Gene:ASE1(YOR058C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Mitotic spindle midzone localized protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress Gene:CIT3(YPR001W)|FD-Score:5.73|P-value:4.94E-9||SGD DESC:Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate Gene:COG5(YNL051W)|FD-Score:-4.61|P-value:2.05E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSR1(YLR380W)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress Gene:DCI1(YOR180C)|FD-Score:5.6|P-value:1.07E-8||SGD DESC:Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed Gene:DFG16(YOR030W)|FD-Score:4.56|P-value:2.55E-6||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DIE2(YGR227W)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:ECM22(YLR228C)|FD-Score:7.7|P-value:7.03E-15||SGD DESC:Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication Gene:EMC2(YJR088C)|FD-Score:3.73|P-value:9.66E-5||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35 Gene:FAA2(YER015W)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome Gene:FLO10(YKR102W)|FD-Score:-3.22|P-value:6.32E-4||SGD DESC:Member of the FLO family of cell wall flocculation proteins; not expressed in most lab strains; overproduction induces flocculation that can be inhibited by mannose, sucrose, or glucose; overproduction also promotes haploid invasive growth and diploid filamentous growth Gene:FYV6(YNL133C)|FD-Score:3.13|P-value:8.86E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GAT3(YLR013W)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Protein containing GATA family zinc finger motifs Gene:GDA1(YEL042W)|FD-Score:-4.28|P-value:9.46E-6||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:GMC2(YLR445W)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor Gene:GND1(YHR183W)|FD-Score:-3.95|P-value:3.99E-5||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:HXT10(YFL011W)|FD-Score:3.86|P-value:5.57E-5||SGD DESC:Putative hexose transporter, expressed at low levels and expression is repressed by glucose Gene:IME1(YJR094C)|FD-Score:8.23|P-value:9.71E-17||SGD DESC:Master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p Gene:IMG2(YCR071C)|FD-Score:-3.64|P-value:1.39E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:IRS4(YKR019C)|FD-Score:-6.13|P-value:4.30E-10||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MAP1(YLR244C)|FD-Score:4.27|P-value:9.56E-6||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MBB1(YJL199C_d)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies Gene:MCH5(YOR306C)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport Gene:MRP13(YGR084C)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSP1(YGR028W)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Mitochondrial protein involved in sorting of proteins in the mitochondria; putative membrane-spanning ATPase Gene:NIT3(YLR351C)|FD-Score:-3.19|P-value:7.14E-4||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:OPI3(YJR073C)|FD-Score:3.1|P-value:9.83E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PET111(YMR257C)|FD-Score:-3.2|P-value:6.98E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PET117(YER058W)|FD-Score:3.87|P-value:5.34E-5||SGD DESC:Protein required for assembly of cytochrome c oxidase Gene:PEX21(YGR239C)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Peroxin required for peroxisomal matrix protein targeting; acts on proteins containing the PTS2 targeting sequence; interacts with Pex7p; partially redundant with Pex18p; relative distribution to cytoplasmic foci increases upon DNA replication stress Gene:PHO89(YBR296C)|FD-Score:-3.92|P-value:4.43E-5||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PIR3(YKL163W)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication Gene:PMD1(YER132C)|FD-Score:4.34|P-value:7.29E-6||SGD DESC:Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions Gene:PTC7(YHR076W)|FD-Score:3.92|P-value:4.50E-5||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:RAD17(YOR368W)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RGP1(YDR137W)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIM9(YMR063W)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RKR1(YMR247C)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice Gene:RMT2(YDR465C)|FD-Score:4.03|P-value:2.77E-5||SGD DESC:Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS10A(YOR293W)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication Gene:RRT2(YBR246W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:SAW1(YAL027W)|FD-Score:-3.73|P-value:9.41E-5||SGD DESC:5'- and 3'-flap DNA binding protein; stimulates the endonuclease activity of Rad1/Rad10p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:SFM1(YOR021C)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism Gene:SLC1(YDL052C)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes Gene:SNF6(YHL025W)|FD-Score:-3.25|P-value:5.76E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SWA2(YDR320C)|FD-Score:-4.16|P-value:1.60E-5||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SWT1(YOR166C)|FD-Score:4.11|P-value:2.01E-5||SGD DESC:RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain Gene:THI73(YLR004C)|FD-Score:-3.38|P-value:3.61E-4||SGD DESC:Putative plasma membrane permease proposed to be involved in carboxylic acid uptake and repressed by thiamine; substrate of Dbf2p/Mob1p kinase; transcription is altered if mitochondrial dysfunction occurs Gene:URN1(YPR152C)|FD-Score:-3.61|P-value:1.53E-4||SGD DESC:Putative protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase Gene:VAC8(YEL013W)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:VPS1(YKR001C)|FD-Score:-4.66|P-value:1.62E-6||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:XDJ1(YLR090W)|FD-Score:4.02|P-value:2.96E-5||SGD DESC:Chaperone with a role in facilitating mitochondrial protein import; ascomycete-specific member of the DnaJ-like family, closely related to Ydj1p; predicted to be C-terminally prenylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YAR029W(YAR029W_p)|FD-Score:3.92|P-value:4.43E-5||SGD DESC:Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YCL074W(YCL074W)|FD-Score:-3.19|P-value:7.18E-4||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YDL159W-A(YDL159W-A_p)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species Gene:YDR391C(YDR391C_p)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YER066C-A(YER066C-A_d)|FD-Score:-6.16|P-value:3.67E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, partially overlaps uncharacterized ORF YER067W Gene:YGL159W(YGL159W_p)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YGL188C-A(YGL188C-A_p)|FD-Score:-3.2|P-value:6.90E-4||SGD DESC:Putative protein of unknown function Gene:YGR079W(YGR079W_p)|FD-Score:4.35|P-value:6.92E-6||SGD DESC:Putative protein of unknown function; YGR079W is not an essential gene Gene:YGR122W(YGR122W)|FD-Score:6.35|P-value:1.09E-10||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YHL042W(YHL042W_p)|FD-Score:-3.24|P-value:6.07E-4||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:YJR005C-A(YJR005C-A_p)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Putative protein of unknown function; originally identified as a syntenic homolog of an <i>Ashbya gossypii</i> gene; YJR005C-A has a paralog, YGR169C-A, that arose from the whole genome duplication Gene:YJR142W(YJR142W_p)|FD-Score:3.16|P-value:7.78E-4||SGD DESC:Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member Gene:YLL056C(YLL056C_p)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:Putative protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p and genes involved in pleiotropic drug resistance (PDR); expression is induced in cells treated with the mycotoxin patulin Gene:YML034C-A(YML034C-A_d)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR105W-A(YMR105W-A_p)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Putative protein of unknown function Gene:YMR158C-A(YMR158C-A_p)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Putative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene Gene:YMR172C-A(YMR172C-A_d)|FD-Score:3.89|P-value:5.06E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNG1(YOR064C)|FD-Score:4.59|P-value:2.26E-6||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1 Gene:YNL338W(YNL338W_d)|FD-Score:-4.17|P-value:1.51E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps TEL14L-XC, which is Telomeric X element Core sequence on the left arm of Chromosome XIV Gene:YOR225W(YOR225W_d)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR078C(YPR078C_p)|FD-Score:3.88|P-value:5.25E-5||SGD DESC:Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible Gene:YPS7(YDR349C)|FD-Score:4.33|P-value:7.43E-6||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YER133W4.483.74E-60.57GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YLR076C_d4.376.29E-60.57YLR076C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10
YDL108W4.181.44E-50.57KIN28Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters
YIL075C3.611.53E-40.39RPN2Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress
YMR013C3.226.34E-40.01SEC59Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation
YKL125W3.226.48E-40.03RRN3Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo
YLR310C3.197.22E-46.77E-4CDC25Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1
YGR002C3.187.24E-40.05SWC4Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex
YKL042W3.148.50E-40.03SPC42Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane
YOR119C3.109.56E-40.19RIO1Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA
YML023C2.910.001790.01NSE5Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
YEL034W2.910.001820.02HYP2Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
YDL031W2.890.001930.00DBP10Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis
YOR372C2.890.001940.03NDD1Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes
YBR136W2.860.002110.10MEC1Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJR094C8.239.71E-17IME1Master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p
YLR228C7.707.03E-15ECM22Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication
YGR122W6.351.09E-10YGR122WProtein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress
YPR001W5.734.94E-9CIT3Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate
YOR180C5.601.07E-8DCI1Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed
YOR064C4.592.26E-6YNG1Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1
YOR030W4.562.55E-6DFG16Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p
YGR079W_p4.356.92E-6YGR079W_pPutative protein of unknown function; YGR079W is not an essential gene
YER132C4.347.29E-6PMD1Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions
YDR349C4.337.43E-6YPS7Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum
YLR244C4.279.56E-6MAP1Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p
YGL071W4.261.01E-5AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YOR166C4.112.01E-5SWT1RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain
YDR465C4.032.77E-5RMT2Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress
YLR090W4.022.96E-5XDJ1Chaperone with a role in facilitating mitochondrial protein import; ascomycete-specific member of the DnaJ-like family, closely related to Ydj1p; predicted to be C-terminally prenylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

GO enrichment analysis for SGTC_345
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1402.88E-27SGTC_349k015-0014 24.0 μMChemDiv (Drug-like library)21969080.111111
0.1164.88E-19SGTC_3464513-0042 7.2 μMChemDiv (Drug-like library)28273720.109589azole & statin
0.1024.70E-15SGTC_22016605497 169.9 μMChembridge (Fragment library)6707410.105263ergosterol biosynthesis
0.0855.78E-11SGTC_3630455-0021 3.1 μMChemDiv (Drug-like library)45974660.09375azole & statin
0.0831.66E-10SGTC_1788fluconazole 20.0 μMMiscellaneous33650.0793651azole & statin
0.0822.54E-10SGTC_28389002687 71.4 μMChembridge (Drug-like library)64570600.151515fatty acid desaturase (OLE1)
0.0822.76E-10SGTC_2784092-0821 23.9 μMChemDiv (Drug-like library)8779000.115385plasma membrane duress
0.0808.31E-10SGTC_9891501-0895 10.3 μMChemDiv (Drug-like library)28301120.121212
0.0791.17E-9SGTC_5761073-0113 22.7 μMChemDiv (Drug-like library)54395630.131579redox potentiating
0.0781.71E-9SGTC_21195528445 120.6 μMChembridge (Fragment library)7919350.140351
0.0782.35E-9SGTC_1067clotrimazole 1.4 μMNIH Clinical Collection28120.101695azole & statin
0.0773.16E-9SGTC_13171273-0028 910.0 nMChemDiv (Drug-like library)59178030.125azole & statin
0.0765.63E-9SGTC_8620452-0056 10.2 μMChemDiv (Drug-like library)15753960.114754azole & statin
0.0741.04E-8SGTC_227fluconazole 33.5 μMMiscellaneous33650.0793651azole & statin
0.0731.88E-8SGTC_30549094397 49.5 μMChembridge (Drug-like library)25983540.131148fatty acid desaturase (OLE1)

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3590081-005815.9 μM0.6578953494159ChemDiv (Drug-like library)250.35814.1020060S ribosome export
SGTC_2181631624054.26 μM0.29545512779990Chembridge (Fragment library)200.233182.50122
SGTC_230MPP+141.67 μM0.28205139484Miscellaneous170.230382.95300
SGTC_15091326-113180 μM0.255319768313ChemDiv (Drug-like library)239.312283.09402
SGTC_550767-02856.56 μM0.2439023116166ChemDiv (Drug-like library)325.446086.93500
SGTC_6090986-002686.3 μM0.2363642848610ChemDiv (Drug-like library)302.38965.17901calcium & mitochondrial duress
SGTC_3231913231349.47 μM0.2363644905967Chembridge (Drug-like library)251.326283.13602
SGTC_20084012253172 μM0.234043603940Chembridge (Fragment library)185.225142.05513
SGTC_1800st05007929.07 μM0.2333333645560TimTec (Natural product derivative library)373.487385.23713NEO1-PIK1
SGTC_400327-010319.75 μM0.222222409036ChemDiv (Drug-like library)324.395125.11701