0133-0054

(2-amino-5-bromophenyl)-phenylmethanone

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_35
Screen concentration 64.1 μM
Source ChemDiv (Drug-like library)
PubChem CID 99677
SMILES C1=CC=C(C=C1)C(=O)C2=C(C=CC(=C2)Br)N
Standardized SMILES Nc1ccc(Br)cc1C(=O)c2ccccc2
Molecular weight 276.1286
ALogP 3.24
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 57.16
% growth inhibition (Hom. pool) 17.92


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 99677
Download HIP data (tab-delimited text)  (excel)
Gene:ALA1(YOR335C)|FD-Score:-4.54|P-value:2.78E-6|Clearance:0||SGD DESC:Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog Gene:APC1(YNL172W)|FD-Score:5.72|P-value:5.43E-9|Clearance:0.72||SGD DESC:Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:BCP1(YDR361C)|FD-Score:3.1|P-value:9.57E-4|Clearance:0.03||SGD DESC:Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport Gene:CDC5(YMR001C)|FD-Score:3.17|P-value:7.56E-4|Clearance:0.06||SGD DESC:Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate Gene:COG4(YPR105C)|FD-Score:-3.71|P-value:1.04E-4|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:FCP1(YMR277W)|FD-Score:3.28|P-value:5.18E-4|Clearance:0.11||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:FOL2(YGR267C)|FD-Score:-3.79|P-value:7.48E-5|Clearance:0||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:HEM2(YGL040C)|FD-Score:3.51|P-value:2.21E-4|Clearance:0.01||SGD DESC:Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus Gene:HYP2(YEL034W)|FD-Score:3.5|P-value:2.32E-4|Clearance:0.09||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:KAR1(YNL188W)|FD-Score:-5.01|P-value:2.79E-7|Clearance:0||SGD DESC:Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p Gene:LCB2(YDR062W)|FD-Score:3.9|P-value:4.76E-5|Clearance:0.05||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:LST8(YNL006W)|FD-Score:3.31|P-value:4.72E-4|Clearance:0.03||SGD DESC:Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat Gene:PBN1(YCL052C)|FD-Score:4.13|P-value:1.84E-5|Clearance:0.1||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:PDS1(YDR113C)|FD-Score:-4.78|P-value:8.59E-7|Clearance:0||SGD DESC:Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:PGS1(YCL004W)|FD-Score:4.88|P-value:5.39E-7|Clearance:0.72||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:PRI1(YIR008C)|FD-Score:-3.38|P-value:3.58E-4|Clearance:0||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RPB5(YBR154C)|FD-Score:4.02|P-value:2.87E-5|Clearance:0.06||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPC11(YDR045C)|FD-Score:5.37|P-value:3.84E-8|Clearance:0.72||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:RPC31(YNL151C)|FD-Score:3.66|P-value:1.28E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C31 Gene:RPL33A(YPL143W)|FD-Score:3.11|P-value:9.28E-4|Clearance:0.01||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:SEC61(YLR378C)|FD-Score:-3.29|P-value:5.05E-4|Clearance:0||SGD DESC:Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER Gene:SLD5(YDR489W)|FD-Score:-3.96|P-value:3.74E-5|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:SMD3(YLR147C)|FD-Score:3.96|P-value:3.70E-5|Clearance:0.06||SGD DESC:Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 Gene:SPC97(YHR172W)|FD-Score:4.15|P-value:1.64E-5|Clearance:0.03||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SRP72(YPL210C)|FD-Score:3.73|P-value:9.45E-5|Clearance:0.08||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:THS1(YIL078W)|FD-Score:-5.68|P-value:6.59E-9|Clearance:0||SGD DESC:Threonyl-tRNA synthetase, essential cytoplasmic protein Gene:TIM17(YJL143W)|FD-Score:3.58|P-value:1.71E-4|Clearance:0.01||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TOA2(YKL058W)|FD-Score:-3.4|P-value:3.31E-4|Clearance:0||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:TRS31(YDR472W)|FD-Score:-3.29|P-value:5.01E-4|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:UTR5(YEL035C_p)|FD-Score:3.57|P-value:1.75E-4|Clearance:0.01||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:YGR265W(YGR265W_d)|FD-Score:3.85|P-value:5.82E-5|Clearance:0.12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:YJL032W(YJL032W_d)|FD-Score:3.41|P-value:3.20E-4|Clearance:0.11||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase Gene:YLR140W(YLR140W_d)|FD-Score:3.57|P-value:1.81E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I Gene:YPL142C(YPL142C_d)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Gene:YTH1(YPR107C)|FD-Score:-5.22|P-value:8.98E-8|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ALA1(YOR335C)|FD-Score:-4.54|P-value:2.78E-6|Clearance:0||SGD DESC:Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog Gene:APC1(YNL172W)|FD-Score:5.72|P-value:5.43E-9|Clearance:0.72||SGD DESC:Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:BCP1(YDR361C)|FD-Score:3.1|P-value:9.57E-4|Clearance:0.03||SGD DESC:Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport Gene:CDC5(YMR001C)|FD-Score:3.17|P-value:7.56E-4|Clearance:0.06||SGD DESC:Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate Gene:COG4(YPR105C)|FD-Score:-3.71|P-value:1.04E-4|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:FCP1(YMR277W)|FD-Score:3.28|P-value:5.18E-4|Clearance:0.11||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:FOL2(YGR267C)|FD-Score:-3.79|P-value:7.48E-5|Clearance:0||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:HEM2(YGL040C)|FD-Score:3.51|P-value:2.21E-4|Clearance:0.01||SGD DESC:Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus Gene:HYP2(YEL034W)|FD-Score:3.5|P-value:2.32E-4|Clearance:0.09||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:KAR1(YNL188W)|FD-Score:-5.01|P-value:2.79E-7|Clearance:0||SGD DESC:Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p Gene:LCB2(YDR062W)|FD-Score:3.9|P-value:4.76E-5|Clearance:0.05||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:LST8(YNL006W)|FD-Score:3.31|P-value:4.72E-4|Clearance:0.03||SGD DESC:Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat Gene:PBN1(YCL052C)|FD-Score:4.13|P-value:1.84E-5|Clearance:0.1||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:PDS1(YDR113C)|FD-Score:-4.78|P-value:8.59E-7|Clearance:0||SGD DESC:Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:PGS1(YCL004W)|FD-Score:4.88|P-value:5.39E-7|Clearance:0.72||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:PRI1(YIR008C)|FD-Score:-3.38|P-value:3.58E-4|Clearance:0||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RPB5(YBR154C)|FD-Score:4.02|P-value:2.87E-5|Clearance:0.06||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPC11(YDR045C)|FD-Score:5.37|P-value:3.84E-8|Clearance:0.72||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:RPC31(YNL151C)|FD-Score:3.66|P-value:1.28E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C31 Gene:RPL33A(YPL143W)|FD-Score:3.11|P-value:9.28E-4|Clearance:0.01||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:SEC61(YLR378C)|FD-Score:-3.29|P-value:5.05E-4|Clearance:0||SGD DESC:Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER Gene:SLD5(YDR489W)|FD-Score:-3.96|P-value:3.74E-5|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:SMD3(YLR147C)|FD-Score:3.96|P-value:3.70E-5|Clearance:0.06||SGD DESC:Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 Gene:SPC97(YHR172W)|FD-Score:4.15|P-value:1.64E-5|Clearance:0.03||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SRP72(YPL210C)|FD-Score:3.73|P-value:9.45E-5|Clearance:0.08||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:THS1(YIL078W)|FD-Score:-5.68|P-value:6.59E-9|Clearance:0||SGD DESC:Threonyl-tRNA synthetase, essential cytoplasmic protein Gene:TIM17(YJL143W)|FD-Score:3.58|P-value:1.71E-4|Clearance:0.01||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TOA2(YKL058W)|FD-Score:-3.4|P-value:3.31E-4|Clearance:0||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:TRS31(YDR472W)|FD-Score:-3.29|P-value:5.01E-4|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:UTR5(YEL035C_p)|FD-Score:3.57|P-value:1.75E-4|Clearance:0.01||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:YGR265W(YGR265W_d)|FD-Score:3.85|P-value:5.82E-5|Clearance:0.12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:YJL032W(YJL032W_d)|FD-Score:3.41|P-value:3.20E-4|Clearance:0.11||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase Gene:YLR140W(YLR140W_d)|FD-Score:3.57|P-value:1.81E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I Gene:YPL142C(YPL142C_d)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Gene:YTH1(YPR107C)|FD-Score:-5.22|P-value:8.98E-8|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 99677
Download HOP data (tab-delimited text)  (excel)
Gene:ADE17(YMR120C)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine Gene:ADH5(YBR145W)|FD-Score:4.67|P-value:1.48E-6||SGD DESC:Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication Gene:AHC2(YCR082W)|FD-Score:5.19|P-value:1.04E-7||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:ALG12(YNR030W)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:APE4(YHR113W)|FD-Score:4.62|P-value:1.95E-6||SGD DESC:Cytoplasmic aspartyl aminopeptidase that may also function in the vacuole; CVT pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family Gene:APQ13(YJL075C_d)|FD-Score:-3.16|P-value:7.76E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene NET1; null mutant is sensitive to sorbate Gene:ASE1(YOR058C)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Mitotic spindle midzone localized protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress Gene:BFA1(YJR053W)|FD-Score:-3.87|P-value:5.40E-5||SGD DESC:Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis Gene:BIO5(YNR056C)|FD-Score:4.41|P-value:5.14E-6||SGD DESC:Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CHS6(YJL099W)|FD-Score:3.96|P-value:3.78E-5||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:COG6(YNL041C)|FD-Score:4.34|P-value:7.12E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTK1(YKL139W)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:CTP1(YBR291C)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family Gene:CUP9(YPL177C)|FD-Score:-5.09|P-value:1.75E-7||SGD DESC:Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:DAL3(YIR032C)|FD-Score:-3.84|P-value:6.17E-5||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DAL81(YIR023W)|FD-Score:4|P-value:3.21E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DNL4(YOR005C)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth Gene:ERG2(YMR202W)|FD-Score:7.69|P-value:7.52E-15||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FMP25(YLR077W)|FD-Score:3.09|P-value:9.87E-4||SGD DESC:Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria Gene:GCR2(YNL199C)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GCS1(YDL226C)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GPD1(YDL022W)|FD-Score:5.13|P-value:1.44E-7||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:GRE1(YPL223C)|FD-Score:-6.24|P-value:2.16E-10||SGD DESC:Hydrophilin essential in dessication-rehydration process; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway Gene:GUF1(YLR289W)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor Gene:HAM1(YJR069C)|FD-Score:3.91|P-value:4.63E-5||SGD DESC:Nucleoside triphosphate pyrophosphohydrolase; active against a wide range of substrates including ITP, dITP and XTP; mediates exclusion of noncanonical purines and pyrimadines from deoxyribonucleoside triphosphate pools; mutant is sensitive to the base analog 6-N-hydroxylaminopurine; protein abundance increases in response to DNA replication stress Gene:HSE1(YHL002W)|FD-Score:-3.2|P-value:6.88E-4||SGD DESC:Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes Gene:HXT8(YJL214W)|FD-Score:4.42|P-value:5.03E-6||SGD DESC:Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:ICP55(YER078C)|FD-Score:4.35|P-value:6.79E-6||SGD DESC:Mitochondrial aminopeptidase; cleaves the N termini of at least 38 imported proteins after cleavage by the mitochondrial processing peptidase (MPP), thereby increasing their stability; member of the aminopeptidase P family Gene:ILM1(YJR118C)|FD-Score:-3.38|P-value:3.68E-4||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:IRC4(YDR540C)|FD-Score:3.86|P-value:5.57E-5||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:JNM1(YMR294W)|FD-Score:4.4|P-value:5.47E-6||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LDB19(YOR322C)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LEU9(YOR108W)|FD-Score:-3.1|P-value:9.65E-4||SGD DESC:Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication Gene:MDM30(YLR368W)|FD-Score:5.66|P-value:7.67E-9||SGD DESC:F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains Gene:MEC3(YLR288C)|FD-Score:-3.24|P-value:5.95E-4||SGD DESC:DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 Gene:MEI4(YER044C-A)|FD-Score:-3.13|P-value:8.79E-4||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MFT1(YML062C)|FD-Score:4.31|P-value:8.19E-6||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MND2(YIR025W)|FD-Score:4.01|P-value:3.05E-5||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MOS2(YGR235C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Mitochondrial inner membrane protein; non-essential component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:MRPL37(YBR268W)|FD-Score:-3.15|P-value:8.28E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSC1(YML128C)|FD-Score:-3.48|P-value:2.50E-4||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated Gene:MSH3(YCR092C)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:NAM8(YHR086W)|FD-Score:-3.53|P-value:2.06E-4||SGD DESC:RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function Gene:NCR1(YPL006W)|FD-Score:5.14|P-value:1.36E-7||SGD DESC:Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein Gene:NEM1(YHR004C)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:PAH1(YMR165C)|FD-Score:-3.21|P-value:6.74E-4||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PFA4(YOL003C)|FD-Score:3.88|P-value:5.12E-5||SGD DESC:Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases Gene:PFS1(YHR185C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation Gene:PIM1(YBL022C)|FD-Score:4.48|P-value:3.69E-6||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PNP1(YLR209C)|FD-Score:-3.29|P-value:5.09E-4||SGD DESC:Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway Gene:PRS2(YER099C)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication Gene:PSY4(YBL046W)|FD-Score:3.87|P-value:5.40E-5||SGD DESC:Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2 Gene:PUS6(YGR169C)|FD-Score:-3.22|P-value:6.49E-4||SGD DESC:tRNA:pseudouridine synthase, catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability Gene:QRI5(YLR204W)|FD-Score:-5.57|P-value:1.28E-8||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RAD18(YCR066W)|FD-Score:3.89|P-value:4.99E-5||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD51(YER095W)|FD-Score:-3.72|P-value:9.79E-5||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RFM1(YOR279C)|FD-Score:-3.79|P-value:7.49E-5||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RGL1(YPL066W)|FD-Score:6.26|P-value:1.97E-10||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RGT1(YKL038W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:RPS22A(YJL190C)|FD-Score:4.51|P-value:3.27E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RTA1(YGR213C)|FD-Score:-4.21|P-value:1.29E-5||SGD DESC:Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein Gene:SET4(YJL105W)|FD-Score:-3.96|P-value:3.70E-5||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SGE1(YPR198W)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SLH1(YGR271W)|FD-Score:-3.74|P-value:9.09E-5||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SLX5(YDL013W)|FD-Score:-4.23|P-value:1.14E-5||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SMY2(YBR172C)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Protein of unknown function involved in COPII vesicle formation; interacts with the Sec23p/Sec24p subcomplex; overexpression suppresses the temperature sensitivity of a myo2 mutant; similar to S. pombe Mpd2; SMY2 has a paralog, SYH1, that arose from the whole genome duplication Gene:SPO22(YIL073C)|FD-Score:-5.63|P-value:8.96E-9||SGD DESC:Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis Gene:STP22(YCL008C)|FD-Score:3.74|P-value:9.32E-5||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:THI22(YPR121W)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:Protein with similarity to hydroxymethylpyrimidine phosphate kinases; member of a gene family with THI20 and THI21; not required for thiamine biosynthesis Gene:THR1(YHR025W)|FD-Score:-5.45|P-value:2.49E-8||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TLG2(YOL018C)|FD-Score:4.59|P-value:2.26E-6||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TNA1(YGR260W)|FD-Score:3.42|P-value:3.19E-4||SGD DESC:High affinity nicotinic acid plasma membrane permease, responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA) Gene:TPM2(YIL138C)|FD-Score:3.99|P-value:3.36E-5||SGD DESC:Minor isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p; TPM2 has a paralog, TPM1, that arose from the whole genome duplication Gene:TRX1(YLR043C)|FD-Score:-3.24|P-value:5.90E-4||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:TUS1(YLR425W)|FD-Score:5.79|P-value:3.46E-9||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:ULA1(YPL003W)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation Gene:VBA2(YBR293W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:WSC3(YOL105C)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity; involved in the response to heat shock and other stressors; regulates 1,3-beta-glucan synthesis Gene:YAR030C(YAR030C_d)|FD-Score:-3.16|P-value:8.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAR029W and the verified gene PRM9 Gene:YAT1(YAR035W)|FD-Score:-4.13|P-value:1.79E-5||SGD DESC:Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated Gene:YBR182C-A(YBR182C-A_p)|FD-Score:3.9|P-value:4.89E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR242W(YBR242W_p)|FD-Score:-3.28|P-value:5.25E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YDL071C(YDL071C_d)|FD-Score:-3.96|P-value:3.78E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W Gene:YDL085C-A(YDL085C-A_p)|FD-Score:-4.5|P-value:3.43E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YDR034W-B(YDR034W-B_p)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR524W-C(YDR524W-C_p)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin Gene:YGK3(YOL128C)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YGL214W(YGL214W_d)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C Gene:YGR012W(YGR012W)|FD-Score:5.29|P-value:6.13E-8||SGD DESC:Putative cysteine synthase, localized to the mitochondrial outer membrane Gene:YGR064W(YGR064W_d)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YHL005C(YHL005C_d)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YHL004W Gene:YHR033W(YHR033W_p)|FD-Score:-3.83|P-value:6.39E-5||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YHR033W has a paralog, PRO1, that arose from the whole genome duplication Gene:YIL086C(YIL086C_d)|FD-Score:-4.77|P-value:9.04E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIR024C(YIR024C)|FD-Score:4.59|P-value:2.27E-6||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect Gene:YJL169W(YJL169W_d)|FD-Score:-3.31|P-value:4.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Gene:YLR342W-A(YLR342W-A_p)|FD-Score:5.12|P-value:1.53E-7||SGD DESC:Putative protein of unknown function Gene:YMR087W(YMR087W)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain Gene:YMR114C(YMR114C)|FD-Score:-3.79|P-value:7.43E-5||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene Gene:YMR158W-B(YMR158W-B_d)|FD-Score:-4.3|P-value:8.53E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene ATG16/YMR159C Gene:YNL122C(YNL122C_p)|FD-Score:4.45|P-value:4.37E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YNR062C(YNR062C_p)|FD-Score:5.34|P-value:4.65E-8||SGD DESC:Putative membrane protein of unknown function Gene:YNR064C(YNR064C)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:YNR065C(YNR065C_p)|FD-Score:4.5|P-value:3.39E-6||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOR225W(YOR225W_d)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR289W(YOR289W_p)|FD-Score:-3.6|P-value:1.59E-4||SGD DESC:Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPL034W(YPL034W_p)|FD-Score:-4.31|P-value:8.20E-6||SGD DESC:Putative protein of unknown function; YPL034W is not essential gene Gene:YPL102C(YPL102C_d)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Gene:YPR059C(YPR059C_d)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W Gene:YPR096C(YPR096C)|FD-Score:6.5|P-value:4.08E-11||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YPR097W(YPR097W)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPS6(YIR039C)|FD-Score:4.94|P-value:3.85E-7||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance Gene:ZIP1(YDR285W)|FD-Score:-4.79|P-value:8.28E-7||SGD DESC:Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate Gene:ADE17(YMR120C)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine Gene:ADH5(YBR145W)|FD-Score:4.67|P-value:1.48E-6||SGD DESC:Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication Gene:AHC2(YCR082W)|FD-Score:5.19|P-value:1.04E-7||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:ALG12(YNR030W)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:APE4(YHR113W)|FD-Score:4.62|P-value:1.95E-6||SGD DESC:Cytoplasmic aspartyl aminopeptidase that may also function in the vacuole; CVT pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family Gene:APQ13(YJL075C_d)|FD-Score:-3.16|P-value:7.76E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene NET1; null mutant is sensitive to sorbate Gene:ASE1(YOR058C)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Mitotic spindle midzone localized protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress Gene:BFA1(YJR053W)|FD-Score:-3.87|P-value:5.40E-5||SGD DESC:Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis Gene:BIO5(YNR056C)|FD-Score:4.41|P-value:5.14E-6||SGD DESC:Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CHS6(YJL099W)|FD-Score:3.96|P-value:3.78E-5||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:COG6(YNL041C)|FD-Score:4.34|P-value:7.12E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTK1(YKL139W)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:CTP1(YBR291C)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family Gene:CUP9(YPL177C)|FD-Score:-5.09|P-value:1.75E-7||SGD DESC:Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:DAL3(YIR032C)|FD-Score:-3.84|P-value:6.17E-5||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DAL81(YIR023W)|FD-Score:4|P-value:3.21E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DNL4(YOR005C)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth Gene:ERG2(YMR202W)|FD-Score:7.69|P-value:7.52E-15||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FMP25(YLR077W)|FD-Score:3.09|P-value:9.87E-4||SGD DESC:Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria Gene:GCR2(YNL199C)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GCS1(YDL226C)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GPD1(YDL022W)|FD-Score:5.13|P-value:1.44E-7||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:GRE1(YPL223C)|FD-Score:-6.24|P-value:2.16E-10||SGD DESC:Hydrophilin essential in dessication-rehydration process; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway Gene:GUF1(YLR289W)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor Gene:HAM1(YJR069C)|FD-Score:3.91|P-value:4.63E-5||SGD DESC:Nucleoside triphosphate pyrophosphohydrolase; active against a wide range of substrates including ITP, dITP and XTP; mediates exclusion of noncanonical purines and pyrimadines from deoxyribonucleoside triphosphate pools; mutant is sensitive to the base analog 6-N-hydroxylaminopurine; protein abundance increases in response to DNA replication stress Gene:HSE1(YHL002W)|FD-Score:-3.2|P-value:6.88E-4||SGD DESC:Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes Gene:HXT8(YJL214W)|FD-Score:4.42|P-value:5.03E-6||SGD DESC:Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:ICP55(YER078C)|FD-Score:4.35|P-value:6.79E-6||SGD DESC:Mitochondrial aminopeptidase; cleaves the N termini of at least 38 imported proteins after cleavage by the mitochondrial processing peptidase (MPP), thereby increasing their stability; member of the aminopeptidase P family Gene:ILM1(YJR118C)|FD-Score:-3.38|P-value:3.68E-4||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:IRC4(YDR540C)|FD-Score:3.86|P-value:5.57E-5||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:JNM1(YMR294W)|FD-Score:4.4|P-value:5.47E-6||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LDB19(YOR322C)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LEU9(YOR108W)|FD-Score:-3.1|P-value:9.65E-4||SGD DESC:Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication Gene:MDM30(YLR368W)|FD-Score:5.66|P-value:7.67E-9||SGD DESC:F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains Gene:MEC3(YLR288C)|FD-Score:-3.24|P-value:5.95E-4||SGD DESC:DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 Gene:MEI4(YER044C-A)|FD-Score:-3.13|P-value:8.79E-4||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MFT1(YML062C)|FD-Score:4.31|P-value:8.19E-6||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MND2(YIR025W)|FD-Score:4.01|P-value:3.05E-5||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MOS2(YGR235C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Mitochondrial inner membrane protein; non-essential component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:MRPL37(YBR268W)|FD-Score:-3.15|P-value:8.28E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSC1(YML128C)|FD-Score:-3.48|P-value:2.50E-4||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated Gene:MSH3(YCR092C)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:NAM8(YHR086W)|FD-Score:-3.53|P-value:2.06E-4||SGD DESC:RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function Gene:NCR1(YPL006W)|FD-Score:5.14|P-value:1.36E-7||SGD DESC:Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein Gene:NEM1(YHR004C)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:PAH1(YMR165C)|FD-Score:-3.21|P-value:6.74E-4||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PFA4(YOL003C)|FD-Score:3.88|P-value:5.12E-5||SGD DESC:Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases Gene:PFS1(YHR185C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation Gene:PIM1(YBL022C)|FD-Score:4.48|P-value:3.69E-6||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PNP1(YLR209C)|FD-Score:-3.29|P-value:5.09E-4||SGD DESC:Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway Gene:PRS2(YER099C)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication Gene:PSY4(YBL046W)|FD-Score:3.87|P-value:5.40E-5||SGD DESC:Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2 Gene:PUS6(YGR169C)|FD-Score:-3.22|P-value:6.49E-4||SGD DESC:tRNA:pseudouridine synthase, catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability Gene:QRI5(YLR204W)|FD-Score:-5.57|P-value:1.28E-8||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RAD18(YCR066W)|FD-Score:3.89|P-value:4.99E-5||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD51(YER095W)|FD-Score:-3.72|P-value:9.79E-5||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RFM1(YOR279C)|FD-Score:-3.79|P-value:7.49E-5||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RGL1(YPL066W)|FD-Score:6.26|P-value:1.97E-10||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RGT1(YKL038W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:RPS22A(YJL190C)|FD-Score:4.51|P-value:3.27E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RTA1(YGR213C)|FD-Score:-4.21|P-value:1.29E-5||SGD DESC:Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein Gene:SET4(YJL105W)|FD-Score:-3.96|P-value:3.70E-5||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SGE1(YPR198W)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SLH1(YGR271W)|FD-Score:-3.74|P-value:9.09E-5||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SLX5(YDL013W)|FD-Score:-4.23|P-value:1.14E-5||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SMY2(YBR172C)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Protein of unknown function involved in COPII vesicle formation; interacts with the Sec23p/Sec24p subcomplex; overexpression suppresses the temperature sensitivity of a myo2 mutant; similar to S. pombe Mpd2; SMY2 has a paralog, SYH1, that arose from the whole genome duplication Gene:SPO22(YIL073C)|FD-Score:-5.63|P-value:8.96E-9||SGD DESC:Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis Gene:STP22(YCL008C)|FD-Score:3.74|P-value:9.32E-5||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:THI22(YPR121W)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:Protein with similarity to hydroxymethylpyrimidine phosphate kinases; member of a gene family with THI20 and THI21; not required for thiamine biosynthesis Gene:THR1(YHR025W)|FD-Score:-5.45|P-value:2.49E-8||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TLG2(YOL018C)|FD-Score:4.59|P-value:2.26E-6||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TNA1(YGR260W)|FD-Score:3.42|P-value:3.19E-4||SGD DESC:High affinity nicotinic acid plasma membrane permease, responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA) Gene:TPM2(YIL138C)|FD-Score:3.99|P-value:3.36E-5||SGD DESC:Minor isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p; TPM2 has a paralog, TPM1, that arose from the whole genome duplication Gene:TRX1(YLR043C)|FD-Score:-3.24|P-value:5.90E-4||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:TUS1(YLR425W)|FD-Score:5.79|P-value:3.46E-9||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:ULA1(YPL003W)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation Gene:VBA2(YBR293W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:WSC3(YOL105C)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity; involved in the response to heat shock and other stressors; regulates 1,3-beta-glucan synthesis Gene:YAR030C(YAR030C_d)|FD-Score:-3.16|P-value:8.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAR029W and the verified gene PRM9 Gene:YAT1(YAR035W)|FD-Score:-4.13|P-value:1.79E-5||SGD DESC:Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated Gene:YBR182C-A(YBR182C-A_p)|FD-Score:3.9|P-value:4.89E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR242W(YBR242W_p)|FD-Score:-3.28|P-value:5.25E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YDL071C(YDL071C_d)|FD-Score:-3.96|P-value:3.78E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W Gene:YDL085C-A(YDL085C-A_p)|FD-Score:-4.5|P-value:3.43E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YDR034W-B(YDR034W-B_p)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR524W-C(YDR524W-C_p)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin Gene:YGK3(YOL128C)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YGL214W(YGL214W_d)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C Gene:YGR012W(YGR012W)|FD-Score:5.29|P-value:6.13E-8||SGD DESC:Putative cysteine synthase, localized to the mitochondrial outer membrane Gene:YGR064W(YGR064W_d)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YHL005C(YHL005C_d)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YHL004W Gene:YHR033W(YHR033W_p)|FD-Score:-3.83|P-value:6.39E-5||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YHR033W has a paralog, PRO1, that arose from the whole genome duplication Gene:YIL086C(YIL086C_d)|FD-Score:-4.77|P-value:9.04E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIR024C(YIR024C)|FD-Score:4.59|P-value:2.27E-6||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect Gene:YJL169W(YJL169W_d)|FD-Score:-3.31|P-value:4.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Gene:YLR342W-A(YLR342W-A_p)|FD-Score:5.12|P-value:1.53E-7||SGD DESC:Putative protein of unknown function Gene:YMR087W(YMR087W)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain Gene:YMR114C(YMR114C)|FD-Score:-3.79|P-value:7.43E-5||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene Gene:YMR158W-B(YMR158W-B_d)|FD-Score:-4.3|P-value:8.53E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene ATG16/YMR159C Gene:YNL122C(YNL122C_p)|FD-Score:4.45|P-value:4.37E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YNR062C(YNR062C_p)|FD-Score:5.34|P-value:4.65E-8||SGD DESC:Putative membrane protein of unknown function Gene:YNR064C(YNR064C)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:YNR065C(YNR065C_p)|FD-Score:4.5|P-value:3.39E-6||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOR225W(YOR225W_d)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR289W(YOR289W_p)|FD-Score:-3.6|P-value:1.59E-4||SGD DESC:Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPL034W(YPL034W_p)|FD-Score:-4.31|P-value:8.20E-6||SGD DESC:Putative protein of unknown function; YPL034W is not essential gene Gene:YPL102C(YPL102C_d)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Gene:YPR059C(YPR059C_d)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W Gene:YPR096C(YPR096C)|FD-Score:6.5|P-value:4.08E-11||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YPR097W(YPR097W)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPS6(YIR039C)|FD-Score:4.94|P-value:3.85E-7||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance Gene:ZIP1(YDR285W)|FD-Score:-4.79|P-value:8.28E-7||SGD DESC:Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL172W5.725.43E-90.72APC1Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress
YDR045C5.373.84E-80.72RPC11RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS
YCL004W4.885.39E-70.72PGS1Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis
YHR172W4.151.64E-50.03SPC97Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YCL052C4.131.84E-50.10PBN1Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X
YBR154C4.022.87E-50.06RPB5RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation
YLR147C3.963.70E-50.06SMD3Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3
YDR062W3.904.76E-50.05LCB2Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
YGR265W_d3.855.82E-50.12YGR265W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase
YPL210C3.739.45E-50.08SRP72Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane
YNL151C3.661.28E-40.00RPC31RNA polymerase III subunit C31
YPL142C_d3.651.29E-40.07YPL142C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit
YJL143W3.581.71E-40.01TIM17Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex
YEL035C_p3.571.75E-40.01UTR5_pProtein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions
YLR140W_d3.571.81E-40.05YLR140W_dDubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR202W7.697.52E-15ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YPR096C6.504.08E-11YPR096CProtein of unknown function that may interact with ribosomes, based on co-purification experiments
YPL066W6.261.97E-10RGL1Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
YLR425W5.793.46E-9TUS1Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate
YLR368W5.667.67E-9MDM30F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains
YNR062C_p5.344.65E-8YNR062C_pPutative membrane protein of unknown function
YGR012W5.296.13E-8YGR012WPutative cysteine synthase, localized to the mitochondrial outer membrane
YCR082W5.191.04E-7AHC2Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex
YPL006W5.141.36E-7NCR1Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein
YDL022W5.131.44E-7GPD1NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity
YLR342W-A_p5.121.53E-7YLR342W-A_pPutative protein of unknown function
YIR039C4.943.85E-7YPS6Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance
YBR145W4.671.48E-6ADH5Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication
YHR113W4.621.95E-6APE4Cytoplasmic aspartyl aminopeptidase that may also function in the vacuole; CVT pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family
YOL018C4.592.26E-6TLG2Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16)

GO enrichment analysis for SGTC_35
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1024.69E-15SGTC_420133-0086 60.7 μMChemDiv (Drug-like library)1629230.638889
0.0961.54E-13SGTC_13601598-0022 39.8 μMChemDiv (Drug-like library)7615250.132075ERG2
0.0952.56E-13SGTC_130199-0087 65.0 μMChemDiv (Drug-like library)45898250.333333ERG2
0.0945.43E-13SGTC_12440416-0015 52.5 μMChemDiv (Drug-like library)962010.0681818ERG2
0.0939.01E-13SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.0909091ERG2
0.0921.48E-12SGTC_20555235184 200.0 μMChembridge (Fragment library)28360340.0588235ERG2
0.0832.07E-10SGTC_7420kpi-0133 140.0 μMChemDiv (Drug-like library)27288520.163636ERG2
0.0791.17E-9SGTC_20265148700 152.0 μMChembridge (Fragment library)798380.0363636
0.0772.89E-9SGTC_6341611-4317 11.2 μMChemDiv (Drug-like library)28435220.116667ERG2
0.0773.71E-9SGTC_9741326-0144 138.0 μMChemDiv (Drug-like library)54118560.0769231ERG2
0.0756.71E-9SGTC_5181021-0119 62.6 μMChemDiv (Drug-like library)10977620.138462ERG2
0.0758.69E-9SGTC_12063978-0017 132.0 μMChemDiv (Drug-like library)38736770.234375ERG2
0.0741.06E-8SGTC_28207989630 71.4 μMChembridge (Drug-like library)29840980.174603ERG2
0.0741.41E-8SGTC_32259130819 49.5 μMChembridge (Drug-like library)45471210.206897amide catabolism
0.0723.77E-8SGTC_254nsc-64875 3.8 μMMiscellaneous56993NAergosterol depletion effects on membrane

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_420133-008660.65 μM0.638889162923ChemDiv (Drug-like library)310.573663.912
SGTC_12700831-117017.3 μM0.4782616764716ChemDiv (Drug-like library)380.234664.25113
SGTC_5651418-000144.5 μM0.4523812862145ChemDiv (Drug-like library)245.704183.63812
SGTC_310508-197457.24 μM0.4313736810525ChemDiv (Drug-like library)414.679724.6213
SGTC_7560508-199928.3 μM0.4038466753853ChemDiv (Drug-like library)394.261244.44213RPP1 & pyrimidine depletion
SGTC_102gallobenzophenone83.62 μM0.39473770837ChemDiv (Drug-like library)230.21612.50834redox potentiating
SGTC_140354-0009295 μM0.3617021713507ChemDiv (Drug-like library)338.626824.51612
SGTC_12640783-0040158 μM0.3488372855798ChemDiv (Drug-like library)339.207642.34613
SGTC_130199-008765 μM0.3333334589825ChemDiv (Drug-like library)354.229326.15902ERG2
SGTC_80354-000170 μM0.324993152ChemDiv (Drug-like library)354.229326.15902