0081-0058

1,2,3,3-tetramethyl-5-phenylindol-1-ium

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_359
Screen concentration 15.9 μM
Source ChemDiv (Drug-like library)
PubChem CID 3494159
SMILES CC1=[N+](C2=C(C1(C)C)C=C(C=C2)C3=CC=CC=C3)C
Standardized SMILES CC1=[N+](C)c2ccc(cc2C1(C)C)c3ccccc3
Molecular weight 250.3581
ALogP 4.1
H-bond donor count 0
H-bond acceptor count 0
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.78
% growth inhibition (Hom. pool) -1.41


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3494159
Download HIP data (tab-delimited text)  (excel)
Gene:BRR2(YER172C)|FD-Score:4.09|P-value:2.19E-5|Clearance:0.05||SGD DESC:RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD Gene:CDC39(YCR093W)|FD-Score:3.8|P-value:7.28E-5|Clearance:0.17||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CDC6(YJL194W)|FD-Score:-6.25|P-value:2.08E-10|Clearance:0||SGD DESC:Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:CKS1(YBR135W)|FD-Score:-4.18|P-value:1.47E-5|Clearance:0||SGD DESC:Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters Gene:CLP1(YOR250C)|FD-Score:3.33|P-value:4.31E-4|Clearance:0.03||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:ESS1(YJR017C)|FD-Score:7.27|P-value:1.79E-13|Clearance:0.97||SGD DESC:Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3 Gene:FBA1(YKL060C)|FD-Score:-3.94|P-value:4.04E-5|Clearance:0||SGD DESC:Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress Gene:FOL1(YNL256W)|FD-Score:3.91|P-value:4.68E-5|Clearance:0.11||SGD DESC:Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities Gene:GUS1(YGL245W)|FD-Score:3.62|P-value:1.45E-4|Clearance:0.03||SGD DESC:Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:LSG1(YGL099W)|FD-Score:11.4|P-value:1.19E-30|Clearance:4.18||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LSM4(YER112W)|FD-Score:3.27|P-value:5.43E-4|Clearance:0.03||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MRD1(YPR112C)|FD-Score:3.42|P-value:3.10E-4|Clearance:0.02||SGD DESC:Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region Gene:NET1(YJL076W)|FD-Score:3.46|P-value:2.67E-4|Clearance:0.04||SGD DESC:Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication Gene:NMD3(YHR170W)|FD-Score:4.82|P-value:7.30E-7|Clearance:0.23||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:ORC4(YPR162C)|FD-Score:-4.71|P-value:1.25E-6|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PCF11(YDR228C)|FD-Score:3.19|P-value:7.17E-4|Clearance:0.05||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:PDS5(YMR076C)|FD-Score:4.28|P-value:9.48E-6|Clearance:0.19||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:PTA1(YAL043C)|FD-Score:-3.36|P-value:3.94E-4|Clearance:0||SGD DESC:Subunit of holo-CPF, a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII Gene:PWP2(YCR057C)|FD-Score:-3.14|P-value:8.52E-4|Clearance:0||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:RPB10(YOR210W)|FD-Score:-3.47|P-value:2.61E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPN11(YFR004W)|FD-Score:4.59|P-value:2.26E-6|Clearance:0.27||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RPN12(YFR052W)|FD-Score:4.04|P-value:2.72E-5|Clearance:0.13||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress Gene:RPO31(YOR116C)|FD-Score:4.97|P-value:3.42E-7|Clearance:0.15||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RRN5(YLR141W)|FD-Score:3.35|P-value:4.04E-4|Clearance:0.02||SGD DESC:Protein involved in transcription of rDNA by RNA polymerase I; transcription factor, member of UAF (upstream activation factor) family along with Rrn9p and Rrn10p Gene:RRP5(YMR229C)|FD-Score:6.3|P-value:1.47E-10|Clearance:0.47||SGD DESC:RNA binding protein involved in synthesis of both 18S and 5.8S rRNAs; component of both the ribosomal small subunit (SSU) processosome and the 90S preribosome; has binding preference for single stranded tracts of U's; relocalizes from nucleolus to nucleus upon DNA replication stress Gene:RTP1(YMR185W_p)|FD-Score:3.23|P-value:6.10E-4|Clearance:0.05||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:SCC2(YDR180W)|FD-Score:-3.48|P-value:2.53E-4|Clearance:0||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin Gene:SCD5(YOR329C)|FD-Score:5.31|P-value:5.53E-8|Clearance:0.34||SGD DESC:Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency Gene:SDH3(YKL141W)|FD-Score:4.09|P-value:2.18E-5|Clearance:0||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex Gene:SMP3(YOR149C)|FD-Score:5.74|P-value:4.81E-9|Clearance:0.43||SGD DESC:Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure Gene:SSC1(YJR045C)|FD-Score:3.37|P-value:3.80E-4|Clearance:0.02||SGD DESC:Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication Gene:TAF5(YBR198C)|FD-Score:3.14|P-value:8.43E-4|Clearance:0.11||SGD DESC:Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:THS1(YIL078W)|FD-Score:3.38|P-value:3.66E-4|Clearance:0.01||SGD DESC:Threonyl-tRNA synthetase, essential cytoplasmic protein Gene:ULP1(YPL020C)|FD-Score:3.31|P-value:4.72E-4|Clearance:0.04||SGD DESC:Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions Gene:UTP23(YOR004W)|FD-Score:5.83|P-value:2.71E-9|Clearance:0.1||SGD DESC:Component of the small subunit processome, involved in 40S ribosomal subunit biogenesis; interacts with snR30 and is required for dissociation of snR30 from large pre-ribosomal particles; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function; essential protein Gene:YDL163W(YDL163W_d)|FD-Score:4.31|P-value:8.09E-6|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase Gene:YDL196W(YDL196W_d)|FD-Score:3.59|P-value:1.62E-4|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YDR341C(YDR341C)|FD-Score:-5.74|P-value:4.66E-9|Clearance:0||SGD DESC:Arginyl-tRNA synthetase, proposed to be cytoplasmic but the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YJL015C(YJL015C_d)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W Gene:YLR458W(YLR458W_d)|FD-Score:-3.17|P-value:7.58E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis Gene:YNL150W(YNL150W_d)|FD-Score:-3.46|P-value:2.70E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking Gene:YRB1(YDR002W)|FD-Score:-3.22|P-value:6.50E-4|Clearance:0||SGD DESC:Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1 Gene:BRR2(YER172C)|FD-Score:4.09|P-value:2.19E-5|Clearance:0.05||SGD DESC:RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD Gene:CDC39(YCR093W)|FD-Score:3.8|P-value:7.28E-5|Clearance:0.17||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CDC6(YJL194W)|FD-Score:-6.25|P-value:2.08E-10|Clearance:0||SGD DESC:Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:CKS1(YBR135W)|FD-Score:-4.18|P-value:1.47E-5|Clearance:0||SGD DESC:Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters Gene:CLP1(YOR250C)|FD-Score:3.33|P-value:4.31E-4|Clearance:0.03||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:ESS1(YJR017C)|FD-Score:7.27|P-value:1.79E-13|Clearance:0.97||SGD DESC:Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3 Gene:FBA1(YKL060C)|FD-Score:-3.94|P-value:4.04E-5|Clearance:0||SGD DESC:Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress Gene:FOL1(YNL256W)|FD-Score:3.91|P-value:4.68E-5|Clearance:0.11||SGD DESC:Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities Gene:GUS1(YGL245W)|FD-Score:3.62|P-value:1.45E-4|Clearance:0.03||SGD DESC:Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:LSG1(YGL099W)|FD-Score:11.4|P-value:1.19E-30|Clearance:4.18||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LSM4(YER112W)|FD-Score:3.27|P-value:5.43E-4|Clearance:0.03||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MRD1(YPR112C)|FD-Score:3.42|P-value:3.10E-4|Clearance:0.02||SGD DESC:Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region Gene:NET1(YJL076W)|FD-Score:3.46|P-value:2.67E-4|Clearance:0.04||SGD DESC:Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication Gene:NMD3(YHR170W)|FD-Score:4.82|P-value:7.30E-7|Clearance:0.23||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:ORC4(YPR162C)|FD-Score:-4.71|P-value:1.25E-6|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PCF11(YDR228C)|FD-Score:3.19|P-value:7.17E-4|Clearance:0.05||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:PDS5(YMR076C)|FD-Score:4.28|P-value:9.48E-6|Clearance:0.19||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:PTA1(YAL043C)|FD-Score:-3.36|P-value:3.94E-4|Clearance:0||SGD DESC:Subunit of holo-CPF, a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII Gene:PWP2(YCR057C)|FD-Score:-3.14|P-value:8.52E-4|Clearance:0||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:RPB10(YOR210W)|FD-Score:-3.47|P-value:2.61E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPN11(YFR004W)|FD-Score:4.59|P-value:2.26E-6|Clearance:0.27||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RPN12(YFR052W)|FD-Score:4.04|P-value:2.72E-5|Clearance:0.13||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress Gene:RPO31(YOR116C)|FD-Score:4.97|P-value:3.42E-7|Clearance:0.15||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RRN5(YLR141W)|FD-Score:3.35|P-value:4.04E-4|Clearance:0.02||SGD DESC:Protein involved in transcription of rDNA by RNA polymerase I; transcription factor, member of UAF (upstream activation factor) family along with Rrn9p and Rrn10p Gene:RRP5(YMR229C)|FD-Score:6.3|P-value:1.47E-10|Clearance:0.47||SGD DESC:RNA binding protein involved in synthesis of both 18S and 5.8S rRNAs; component of both the ribosomal small subunit (SSU) processosome and the 90S preribosome; has binding preference for single stranded tracts of U's; relocalizes from nucleolus to nucleus upon DNA replication stress Gene:RTP1(YMR185W_p)|FD-Score:3.23|P-value:6.10E-4|Clearance:0.05||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:SCC2(YDR180W)|FD-Score:-3.48|P-value:2.53E-4|Clearance:0||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin Gene:SCD5(YOR329C)|FD-Score:5.31|P-value:5.53E-8|Clearance:0.34||SGD DESC:Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency Gene:SDH3(YKL141W)|FD-Score:4.09|P-value:2.18E-5|Clearance:0||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex Gene:SMP3(YOR149C)|FD-Score:5.74|P-value:4.81E-9|Clearance:0.43||SGD DESC:Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure Gene:SSC1(YJR045C)|FD-Score:3.37|P-value:3.80E-4|Clearance:0.02||SGD DESC:Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication Gene:TAF5(YBR198C)|FD-Score:3.14|P-value:8.43E-4|Clearance:0.11||SGD DESC:Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:THS1(YIL078W)|FD-Score:3.38|P-value:3.66E-4|Clearance:0.01||SGD DESC:Threonyl-tRNA synthetase, essential cytoplasmic protein Gene:ULP1(YPL020C)|FD-Score:3.31|P-value:4.72E-4|Clearance:0.04||SGD DESC:Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions Gene:UTP23(YOR004W)|FD-Score:5.83|P-value:2.71E-9|Clearance:0.1||SGD DESC:Component of the small subunit processome, involved in 40S ribosomal subunit biogenesis; interacts with snR30 and is required for dissociation of snR30 from large pre-ribosomal particles; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function; essential protein Gene:YDL163W(YDL163W_d)|FD-Score:4.31|P-value:8.09E-6|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase Gene:YDL196W(YDL196W_d)|FD-Score:3.59|P-value:1.62E-4|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YDR341C(YDR341C)|FD-Score:-5.74|P-value:4.66E-9|Clearance:0||SGD DESC:Arginyl-tRNA synthetase, proposed to be cytoplasmic but the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YJL015C(YJL015C_d)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W Gene:YLR458W(YLR458W_d)|FD-Score:-3.17|P-value:7.58E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis Gene:YNL150W(YNL150W_d)|FD-Score:-3.46|P-value:2.70E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking Gene:YRB1(YDR002W)|FD-Score:-3.22|P-value:6.50E-4|Clearance:0||SGD DESC:Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3494159
Download HOP data (tab-delimited text)  (excel)
Gene:AAH1(YNL141W)|FD-Score:-3.33|P-value:4.39E-4||SGD DESC:Adenine deaminase (adenine aminohydrolase), converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome Gene:AAT2(YLR027C)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ACA1(YER045C)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:ATF/CREB family basic leucine zipper (bZIP) transcription factor; binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA; important for carbon source utilization; target genes include GRE2 and COS8; ACA1 has a paralog, CST6, that arose from the whole genome duplication Gene:ADA2(YDR448W)|FD-Score:-5.52|P-value:1.70E-8||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:ALD2(YMR170C)|FD-Score:4.4|P-value:5.33E-6||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:APL6(YGR261C)|FD-Score:-14|P-value:5.20E-45||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:BEM4(YPL161C)|FD-Score:3.79|P-value:7.62E-5||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BTS1(YPL069C)|FD-Score:-3.79|P-value:7.68E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CBP4(YGR174C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex Gene:CDC73(YLR418C)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CLB6(YGR109C)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 Gene:COG5(YNL051W)|FD-Score:-3.66|P-value:1.24E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX23(YHR116W)|FD-Score:-3.74|P-value:9.08E-5||SGD DESC:Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs Gene:CPR8(YNR028W)|FD-Score:4.34|P-value:7.00E-6||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway Gene:CTK1(YKL139W)|FD-Score:-3.92|P-value:4.37E-5||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:CUE3(YGL110C)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination Gene:DCW1(YKL046C)|FD-Score:3.22|P-value:6.30E-4||SGD DESC:Putative mannosidase, GPI-anchored membrane protein required for cell wall biosynthesis in bud formation;homologous to Dfg5p Gene:DDR2(YOL052C-A)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:DPP1(YDR284C)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:DUG2(YBR281C)|FD-Score:5.22|P-value:8.72E-8||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:EDS1(YBR033W_p)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:EEB1(YPL095C)|FD-Score:-4.48|P-value:3.73E-6||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication Gene:ELP2(YGR200C)|FD-Score:-4.35|P-value:6.71E-6||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:ERI1(YPL096C-A)|FD-Score:-4.54|P-value:2.86E-6||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:FKH1(YIL131C)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Forkhead family transcription factor; has a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; binds to the recombination enhancer near HML, and regulates donor preference during mating-type switching by positioning HML near the MAT locus through FHA-domain mediated interactions with proteins near the DSB; FKH1 has a paralog, FKH2, that arose from the whole genome duplication Gene:FLO10(YKR102W)|FD-Score:3.86|P-value:5.77E-5||SGD DESC:Member of the FLO family of cell wall flocculation proteins; not expressed in most lab strains; overproduction induces flocculation that can be inhibited by mannose, sucrose, or glucose; overproduction also promotes haploid invasive growth and diploid filamentous growth Gene:FLO8(YER109C)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene Gene:FMP10(YER182W_p)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRD1(YEL047C)|FD-Score:-3.82|P-value:6.57E-5||SGD DESC:Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication Gene:FRE1(YLR214W)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low copper and iron levels Gene:GDE1(YPL110C)|FD-Score:4.28|P-value:9.39E-6||SGD DESC:Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes Gene:GPT2(YKR067W)|FD-Score:-5.78|P-value:3.79E-9||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:GTR2(YGR163W)|FD-Score:4.42|P-value:4.90E-6||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:GUT1(YHL032C)|FD-Score:12.7|P-value:3.37E-37||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:HRB1(YNL004W)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; HRB1 has a paralog, GBP2, that arose from the whole genome duplication Gene:IDH2(YOR136W)|FD-Score:11.3|P-value:8.84E-30||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IES2(YNL215W)|FD-Score:7.44|P-value:5.01E-14||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:IES5(YER092W)|FD-Score:-3.36|P-value:3.91E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions Gene:IGO2(YHR132W-A)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo1p Gene:IMG2(YCR071C)|FD-Score:-5.87|P-value:2.13E-9||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:IML2(YJL082W)|FD-Score:7.24|P-value:2.24E-13||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication Gene:IRC8(YJL051W)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Bud tip localized protein of unknown function; mRNA is targeted to the bud by a She2p dependent transport system; mRNA is cell cycle regulated via Fkh2p, peaking in G2/M phase; null mutant displays increased levels of spontaneous Rad52p foc Gene:ISU1(YPL135W)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable Gene:KTI11(YBL071W-A)|FD-Score:5.45|P-value:2.48E-8||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:LIF1(YGL090W)|FD-Score:3.94|P-value:4.02E-5||SGD DESC:Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein Gene:LYP1(YNL268W)|FD-Score:10|P-value:6.40E-24||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:MDH1(YKL085W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated Gene:NAP1(YKR048C)|FD-Score:3.84|P-value:6.16E-5||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:NDI1(YML120C)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:NADH:ubiquinone oxidoreductase; transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; phosphorylated; involved in Mn and H2O2 induced apoptosis; upon apoptotic stress, Ndip is activated in the mitochondria by N-terminal cleavage, and the truncated protein translocates to the cytoplasm to induce apoptosis; homolog of human AMID Gene:NMD4(YLR363C)|FD-Score:4.43|P-value:4.72E-6||SGD DESC:Protein that may be involved in nonsense-mediated mRNA decay; interacts with Nam7p, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NUC1(YJL208C)|FD-Score:4.98|P-value:3.14E-7||SGD DESC:Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy Gene:OSH3(YHR073W)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PAD1(YDR538W)|FD-Score:4.94|P-value:3.85E-7||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PAM17(YKR065C)|FD-Score:-4.36|P-value:6.40E-6||SGD DESC:Constituent of the TIM23 complex; proposed alternatively to be a component of the import motor (PAM complex) or to interact with and modulate the core TIM23 (Translocase of the Inner mitochondrial Membrane) complex; protein abundance increases in response to DNA replication stress Gene:PAU11(YGL261C_p)|FD-Score:4.15|P-value:1.64E-5||SGD DESC:Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 Gene:PCA1(YBR295W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function Gene:PDC5(YLR134W)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism Gene:PEX29(YDR479C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PPH3(YDR075W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes Gene:PTC3(YBL056W)|FD-Score:4.49|P-value:3.62E-6||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication Gene:PTC7(YHR076W)|FD-Score:-3.37|P-value:3.74E-4||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:PTR2(YKR093W)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:PUB1(YNL016W)|FD-Score:4.91|P-value:4.45E-7||SGD DESC:Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress Gene:PUF6(YDR496C)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Pumilio-homology domain protein that binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; also co-sediments with the 60S ribosomal subunit and is required for its biogenesis Gene:QCR7(YDR529C)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:QDR1(YIL120W)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for resistance to quinidine, ketoconazole, fluconazole, and barban; QDR1 has a paralog, AQR1, that arose from the whole genome duplication Gene:RAD34(YDR314C)|FD-Score:-3.17|P-value:7.66E-4||SGD DESC:Protein involved in nucleotide excision repair (NER); homologous to RAD4 Gene:RAM1(YDL090C)|FD-Score:-4.44|P-value:4.50E-6||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RGM1(YMR182C)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Putative zinc finger DNA binding transcription factor; contains two N-terminal C2H2 zinc fingers and C-terminal proline rich domain; overproduction impairs cell growth and induces expression of genes involved in monosaccharide catabolism and aldehyde metabolism; regulates expression of of Y-prime telomeric elements and subtelomeric COS genes; RGM1 has a paralog, USV1, that arose from the whole genome duplication Gene:RGT2(YDL138W)|FD-Score:3.81|P-value:6.91E-5||SGD DESC:Plasma membrane high glucose sensor that regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; RGT2 has a paralog, SNF3, that arose from the whole genome duplication Gene:RIM20(YOR275C)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation Gene:RIM4(YHL024W)|FD-Score:-3.56|P-value:1.85E-4||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RPA14(YDR156W)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:RNA polymerase I subunit A14 Gene:RPL24B(YGR148C)|FD-Score:6.71|P-value:9.88E-12||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPL31A(YDL075W)|FD-Score:-5.08|P-value:1.88E-7||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RTS3(YGR161C_p)|FD-Score:3.85|P-value:5.92E-5||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:SER3(YER081W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:SGO1(YOR073W)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SIR1(YKR101W)|FD-Score:4.82|P-value:7.35E-7||SGD DESC:Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin Gene:SKN7(YHR206W)|FD-Score:-4|P-value:3.15E-5||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SLI15(YBR156C)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), which regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly Gene:SPO75(YLL005C)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:SPT4(YGR063C)|FD-Score:-3.28|P-value:5.27E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SRO77(YBL106C)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SUL1(YBR294W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SYN8(YAL014C)|FD-Score:5.12|P-value:1.53E-7||SGD DESC:Endosomal SNARE related to mammalian syntaxin 8 Gene:TDA1(YMR291W)|FD-Score:-3.12|P-value:8.94E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:THR1(YHR025W)|FD-Score:-3.7|P-value:1.06E-4||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TNA1(YGR260W)|FD-Score:-3.16|P-value:7.88E-4||SGD DESC:High affinity nicotinic acid plasma membrane permease, responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA) Gene:TRR2(YHR106W)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Mitochondrial thioredoxin reductase; involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD; TRR2 has a paralog, TRR1, that arose from the whole genome duplication Gene:TUM1(YOR251C)|FD-Score:4.69|P-value:1.35E-6||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:UBA3(YPR066W)|FD-Score:6.56|P-value:2.71E-11||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UBC4(YBR082C)|FD-Score:-3.24|P-value:6.01E-4||SGD DESC:Ubiquitin-conjugating enzyme (E2); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication Gene:VFA1(YER128W)|FD-Score:5.26|P-value:7.37E-8||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:VMA9(YCL005W-A)|FD-Score:5.43|P-value:2.83E-8||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VPS52(YDR484W)|FD-Score:-6.4|P-value:7.92E-11||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:VPS65(YLR322W_d)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:YBR013C(YBR013C_p)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YBR056W(YBR056W_p)|FD-Score:-3.27|P-value:5.42E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YBR139W(YBR139W)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner Gene:YCL074W(YCL074W)|FD-Score:3.8|P-value:7.12E-5||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YCR051W(YCR051W_p)|FD-Score:-3.44|P-value:2.96E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene Gene:YCR085W(YCR085W_d)|FD-Score:-3.27|P-value:5.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR149C(YDR149C_d)|FD-Score:4.63|P-value:1.83E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YER053C-A(YER053C-A_p)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YFL012W(YFL012W_p)|FD-Score:-7.72|P-value:5.94E-15||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YFR018C(YFR018C_p)|FD-Score:-3.3|P-value:4.83E-4||SGD DESC:Putative protein of unknown function Gene:YGR053C(YGR053C_p)|FD-Score:-3.28|P-value:5.10E-4||SGD DESC:Putative protein of unknown function Gene:YGR122C-A(YGR122C-A_d)|FD-Score:4.09|P-value:2.11E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W Gene:YGR137W(YGR137W_d)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR174W-A(YGR174W-A_p)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Putative protein of unknown function; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YIR024C(YIR024C)|FD-Score:-3.37|P-value:3.82E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect Gene:YJR115W(YJR115W_p)|FD-Score:9.09|P-value:5.16E-20||SGD DESC:Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication Gene:YKU80(YMR106C)|FD-Score:5.63|P-value:9.00E-9||SGD DESC:Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair Gene:YLL059C(YLL059C_d)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR307C-A(YLR307C-A_p)|FD-Score:3.86|P-value:5.61E-5||SGD DESC:Putative protein of unknown function Gene:YLR349W(YLR349W_d)|FD-Score:5.84|P-value:2.58E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified ORF DIC1/YLR348C Gene:YLR374C(YLR374C_d)|FD-Score:3.81|P-value:7.08E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YML054C-A(YML054C-A_p)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Putative protein of unknown function Gene:YMR103C(YMR103C_d)|FD-Score:4.86|P-value:5.85E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR141C(YMR141C_d)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR230W-A(YMR230W-A_p)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Putative protein of unknown function Gene:YMR306C-A(YMR306C-A_d)|FD-Score:5.07|P-value:2.00E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL097C-B(YNL097C-B_p)|FD-Score:5.65|P-value:8.23E-9||SGD DESC:Putative protein of unknown function Gene:YOL047C(YOL047C_p)|FD-Score:3.76|P-value:8.52E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YOR1(YGR281W)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:YOR121C(YOR121C_d)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YOR314W(YOR314W_d)|FD-Score:4.29|P-value:8.92E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR376W-A(YOR376W-A_p)|FD-Score:-3.15|P-value:8.16E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPK9(YOR291W)|FD-Score:7.27|P-value:1.73E-13||SGD DESC:Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome Gene:YPL229W(YPL229W_p)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication Gene:YPR027C(YPR027C_p)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Putative protein of unknown function Gene:YPR053C(YPR053C_d)|FD-Score:-7.09|P-value:6.69E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene NHP6A/YPR052C Gene:YPR146C(YPR146C_d)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YTP1(YNL237W)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:AAH1(YNL141W)|FD-Score:-3.33|P-value:4.39E-4||SGD DESC:Adenine deaminase (adenine aminohydrolase), converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome Gene:AAT2(YLR027C)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ACA1(YER045C)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:ATF/CREB family basic leucine zipper (bZIP) transcription factor; binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA; important for carbon source utilization; target genes include GRE2 and COS8; ACA1 has a paralog, CST6, that arose from the whole genome duplication Gene:ADA2(YDR448W)|FD-Score:-5.52|P-value:1.70E-8||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:ALD2(YMR170C)|FD-Score:4.4|P-value:5.33E-6||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:APL6(YGR261C)|FD-Score:-14|P-value:5.20E-45||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:BEM4(YPL161C)|FD-Score:3.79|P-value:7.62E-5||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BTS1(YPL069C)|FD-Score:-3.79|P-value:7.68E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CBP4(YGR174C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex Gene:CDC73(YLR418C)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CLB6(YGR109C)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 Gene:COG5(YNL051W)|FD-Score:-3.66|P-value:1.24E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX23(YHR116W)|FD-Score:-3.74|P-value:9.08E-5||SGD DESC:Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs Gene:CPR8(YNR028W)|FD-Score:4.34|P-value:7.00E-6||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway Gene:CTK1(YKL139W)|FD-Score:-3.92|P-value:4.37E-5||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:CUE3(YGL110C)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination Gene:DCW1(YKL046C)|FD-Score:3.22|P-value:6.30E-4||SGD DESC:Putative mannosidase, GPI-anchored membrane protein required for cell wall biosynthesis in bud formation;homologous to Dfg5p Gene:DDR2(YOL052C-A)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:DPP1(YDR284C)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:DUG2(YBR281C)|FD-Score:5.22|P-value:8.72E-8||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:EDS1(YBR033W_p)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:EEB1(YPL095C)|FD-Score:-4.48|P-value:3.73E-6||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication Gene:ELP2(YGR200C)|FD-Score:-4.35|P-value:6.71E-6||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:ERI1(YPL096C-A)|FD-Score:-4.54|P-value:2.86E-6||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:FKH1(YIL131C)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Forkhead family transcription factor; has a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; binds to the recombination enhancer near HML, and regulates donor preference during mating-type switching by positioning HML near the MAT locus through FHA-domain mediated interactions with proteins near the DSB; FKH1 has a paralog, FKH2, that arose from the whole genome duplication Gene:FLO10(YKR102W)|FD-Score:3.86|P-value:5.77E-5||SGD DESC:Member of the FLO family of cell wall flocculation proteins; not expressed in most lab strains; overproduction induces flocculation that can be inhibited by mannose, sucrose, or glucose; overproduction also promotes haploid invasive growth and diploid filamentous growth Gene:FLO8(YER109C)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene Gene:FMP10(YER182W_p)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRD1(YEL047C)|FD-Score:-3.82|P-value:6.57E-5||SGD DESC:Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication Gene:FRE1(YLR214W)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low copper and iron levels Gene:GDE1(YPL110C)|FD-Score:4.28|P-value:9.39E-6||SGD DESC:Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes Gene:GPT2(YKR067W)|FD-Score:-5.78|P-value:3.79E-9||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:GTR2(YGR163W)|FD-Score:4.42|P-value:4.90E-6||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:GUT1(YHL032C)|FD-Score:12.7|P-value:3.37E-37||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:HRB1(YNL004W)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; HRB1 has a paralog, GBP2, that arose from the whole genome duplication Gene:IDH2(YOR136W)|FD-Score:11.3|P-value:8.84E-30||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IES2(YNL215W)|FD-Score:7.44|P-value:5.01E-14||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:IES5(YER092W)|FD-Score:-3.36|P-value:3.91E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions Gene:IGO2(YHR132W-A)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo1p Gene:IMG2(YCR071C)|FD-Score:-5.87|P-value:2.13E-9||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:IML2(YJL082W)|FD-Score:7.24|P-value:2.24E-13||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication Gene:IRC8(YJL051W)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Bud tip localized protein of unknown function; mRNA is targeted to the bud by a She2p dependent transport system; mRNA is cell cycle regulated via Fkh2p, peaking in G2/M phase; null mutant displays increased levels of spontaneous Rad52p foc Gene:ISU1(YPL135W)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable Gene:KTI11(YBL071W-A)|FD-Score:5.45|P-value:2.48E-8||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:LIF1(YGL090W)|FD-Score:3.94|P-value:4.02E-5||SGD DESC:Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein Gene:LYP1(YNL268W)|FD-Score:10|P-value:6.40E-24||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:MDH1(YKL085W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated Gene:NAP1(YKR048C)|FD-Score:3.84|P-value:6.16E-5||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:NDI1(YML120C)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:NADH:ubiquinone oxidoreductase; transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; phosphorylated; involved in Mn and H2O2 induced apoptosis; upon apoptotic stress, Ndip is activated in the mitochondria by N-terminal cleavage, and the truncated protein translocates to the cytoplasm to induce apoptosis; homolog of human AMID Gene:NMD4(YLR363C)|FD-Score:4.43|P-value:4.72E-6||SGD DESC:Protein that may be involved in nonsense-mediated mRNA decay; interacts with Nam7p, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NUC1(YJL208C)|FD-Score:4.98|P-value:3.14E-7||SGD DESC:Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy Gene:OSH3(YHR073W)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PAD1(YDR538W)|FD-Score:4.94|P-value:3.85E-7||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PAM17(YKR065C)|FD-Score:-4.36|P-value:6.40E-6||SGD DESC:Constituent of the TIM23 complex; proposed alternatively to be a component of the import motor (PAM complex) or to interact with and modulate the core TIM23 (Translocase of the Inner mitochondrial Membrane) complex; protein abundance increases in response to DNA replication stress Gene:PAU11(YGL261C_p)|FD-Score:4.15|P-value:1.64E-5||SGD DESC:Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 Gene:PCA1(YBR295W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function Gene:PDC5(YLR134W)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism Gene:PEX29(YDR479C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PPH3(YDR075W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes Gene:PTC3(YBL056W)|FD-Score:4.49|P-value:3.62E-6||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication Gene:PTC7(YHR076W)|FD-Score:-3.37|P-value:3.74E-4||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:PTR2(YKR093W)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:PUB1(YNL016W)|FD-Score:4.91|P-value:4.45E-7||SGD DESC:Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress Gene:PUF6(YDR496C)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Pumilio-homology domain protein that binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; also co-sediments with the 60S ribosomal subunit and is required for its biogenesis Gene:QCR7(YDR529C)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:QDR1(YIL120W)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for resistance to quinidine, ketoconazole, fluconazole, and barban; QDR1 has a paralog, AQR1, that arose from the whole genome duplication Gene:RAD34(YDR314C)|FD-Score:-3.17|P-value:7.66E-4||SGD DESC:Protein involved in nucleotide excision repair (NER); homologous to RAD4 Gene:RAM1(YDL090C)|FD-Score:-4.44|P-value:4.50E-6||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RGM1(YMR182C)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Putative zinc finger DNA binding transcription factor; contains two N-terminal C2H2 zinc fingers and C-terminal proline rich domain; overproduction impairs cell growth and induces expression of genes involved in monosaccharide catabolism and aldehyde metabolism; regulates expression of of Y-prime telomeric elements and subtelomeric COS genes; RGM1 has a paralog, USV1, that arose from the whole genome duplication Gene:RGT2(YDL138W)|FD-Score:3.81|P-value:6.91E-5||SGD DESC:Plasma membrane high glucose sensor that regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; RGT2 has a paralog, SNF3, that arose from the whole genome duplication Gene:RIM20(YOR275C)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation Gene:RIM4(YHL024W)|FD-Score:-3.56|P-value:1.85E-4||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RPA14(YDR156W)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:RNA polymerase I subunit A14 Gene:RPL24B(YGR148C)|FD-Score:6.71|P-value:9.88E-12||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPL31A(YDL075W)|FD-Score:-5.08|P-value:1.88E-7||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RTS3(YGR161C_p)|FD-Score:3.85|P-value:5.92E-5||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:SER3(YER081W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:SGO1(YOR073W)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SIR1(YKR101W)|FD-Score:4.82|P-value:7.35E-7||SGD DESC:Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin Gene:SKN7(YHR206W)|FD-Score:-4|P-value:3.15E-5||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SLI15(YBR156C)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), which regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly Gene:SPO75(YLL005C)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:SPT4(YGR063C)|FD-Score:-3.28|P-value:5.27E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SRO77(YBL106C)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SUL1(YBR294W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SYN8(YAL014C)|FD-Score:5.12|P-value:1.53E-7||SGD DESC:Endosomal SNARE related to mammalian syntaxin 8 Gene:TDA1(YMR291W)|FD-Score:-3.12|P-value:8.94E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:THR1(YHR025W)|FD-Score:-3.7|P-value:1.06E-4||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TNA1(YGR260W)|FD-Score:-3.16|P-value:7.88E-4||SGD DESC:High affinity nicotinic acid plasma membrane permease, responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA) Gene:TRR2(YHR106W)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Mitochondrial thioredoxin reductase; involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD; TRR2 has a paralog, TRR1, that arose from the whole genome duplication Gene:TUM1(YOR251C)|FD-Score:4.69|P-value:1.35E-6||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:UBA3(YPR066W)|FD-Score:6.56|P-value:2.71E-11||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UBC4(YBR082C)|FD-Score:-3.24|P-value:6.01E-4||SGD DESC:Ubiquitin-conjugating enzyme (E2); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication Gene:VFA1(YER128W)|FD-Score:5.26|P-value:7.37E-8||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:VMA9(YCL005W-A)|FD-Score:5.43|P-value:2.83E-8||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VPS52(YDR484W)|FD-Score:-6.4|P-value:7.92E-11||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:VPS65(YLR322W_d)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:YBR013C(YBR013C_p)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YBR056W(YBR056W_p)|FD-Score:-3.27|P-value:5.42E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YBR139W(YBR139W)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner Gene:YCL074W(YCL074W)|FD-Score:3.8|P-value:7.12E-5||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YCR051W(YCR051W_p)|FD-Score:-3.44|P-value:2.96E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene Gene:YCR085W(YCR085W_d)|FD-Score:-3.27|P-value:5.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR149C(YDR149C_d)|FD-Score:4.63|P-value:1.83E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YER053C-A(YER053C-A_p)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YFL012W(YFL012W_p)|FD-Score:-7.72|P-value:5.94E-15||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YFR018C(YFR018C_p)|FD-Score:-3.3|P-value:4.83E-4||SGD DESC:Putative protein of unknown function Gene:YGR053C(YGR053C_p)|FD-Score:-3.28|P-value:5.10E-4||SGD DESC:Putative protein of unknown function Gene:YGR122C-A(YGR122C-A_d)|FD-Score:4.09|P-value:2.11E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W Gene:YGR137W(YGR137W_d)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR174W-A(YGR174W-A_p)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Putative protein of unknown function; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YIR024C(YIR024C)|FD-Score:-3.37|P-value:3.82E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect Gene:YJR115W(YJR115W_p)|FD-Score:9.09|P-value:5.16E-20||SGD DESC:Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication Gene:YKU80(YMR106C)|FD-Score:5.63|P-value:9.00E-9||SGD DESC:Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair Gene:YLL059C(YLL059C_d)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR307C-A(YLR307C-A_p)|FD-Score:3.86|P-value:5.61E-5||SGD DESC:Putative protein of unknown function Gene:YLR349W(YLR349W_d)|FD-Score:5.84|P-value:2.58E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified ORF DIC1/YLR348C Gene:YLR374C(YLR374C_d)|FD-Score:3.81|P-value:7.08E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YML054C-A(YML054C-A_p)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Putative protein of unknown function Gene:YMR103C(YMR103C_d)|FD-Score:4.86|P-value:5.85E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR141C(YMR141C_d)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR230W-A(YMR230W-A_p)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Putative protein of unknown function Gene:YMR306C-A(YMR306C-A_d)|FD-Score:5.07|P-value:2.00E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL097C-B(YNL097C-B_p)|FD-Score:5.65|P-value:8.23E-9||SGD DESC:Putative protein of unknown function Gene:YOL047C(YOL047C_p)|FD-Score:3.76|P-value:8.52E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YOR1(YGR281W)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:YOR121C(YOR121C_d)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YOR314W(YOR314W_d)|FD-Score:4.29|P-value:8.92E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR376W-A(YOR376W-A_p)|FD-Score:-3.15|P-value:8.16E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPK9(YOR291W)|FD-Score:7.27|P-value:1.73E-13||SGD DESC:Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome Gene:YPL229W(YPL229W_p)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication Gene:YPR027C(YPR027C_p)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Putative protein of unknown function Gene:YPR053C(YPR053C_d)|FD-Score:-7.09|P-value:6.69E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene NHP6A/YPR052C Gene:YPR146C(YPR146C_d)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YTP1(YNL237W)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W11.401.19E-304.18LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YJR017C7.271.79E-130.97ESS1Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3
YMR229C6.301.47E-100.47RRP5RNA binding protein involved in synthesis of both 18S and 5.8S rRNAs; component of both the ribosomal small subunit (SSU) processosome and the 90S preribosome; has binding preference for single stranded tracts of U's; relocalizes from nucleolus to nucleus upon DNA replication stress
YOR004W5.832.71E-90.10UTP23Component of the small subunit processome, involved in 40S ribosomal subunit biogenesis; interacts with snR30 and is required for dissociation of snR30 from large pre-ribosomal particles; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function; essential protein
YOR149C5.744.81E-90.43SMP3Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure
YOR329C5.315.53E-80.34SCD5Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency
YOR116C4.973.42E-70.15RPO31RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
YHR170W4.827.30E-70.23NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YFR004W4.592.26E-60.27RPN11Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
YDL163W_d4.318.09E-60.04YDL163W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase
YMR076C4.289.48E-60.19PDS5Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis
YKL141W4.092.18E-58.89E-4SDH3Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex
YER172C4.092.19E-50.05BRR2RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD
YFR052W4.042.72E-50.13RPN12Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress
YNL256W3.914.68E-50.11FOL1Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHL032C12.703.37E-37GUT1Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p
YOR136W11.308.84E-30IDH2Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated
YNL268W10.006.40E-24LYP1Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids
YJR115W_p9.095.16E-20YJR115W_pPutative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication
YNL215W7.445.01E-14IES2Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress
YOR291W7.271.73E-13YPK9Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome
YJL082W7.242.24E-13IML2Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication
YGR148C6.719.88E-12RPL24BRibosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication
YPR066W6.562.71E-11UBA3Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern
YLR349W_d5.842.58E-9YLR349W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified ORF DIC1/YLR348C
YNL097C-B_p5.658.23E-9YNL097C-B_pPutative protein of unknown function
YMR106C5.639.00E-9YKU80Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair
YBL071W-A5.452.48E-8KTI11Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs
YCL005W-A5.432.83E-8VMA9Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis
YER128W5.267.37E-8VFA1Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology

GO enrichment analysis for SGTC_359
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1233.16E-21SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.073170760S ribosome export
0.1224.31E-21SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.096774260S ribosome export
0.1187.25E-20SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.081081160S ribosome export
0.1071.97E-16SGTC_1522st012842 45.6 μMTimTec (Pure natural product library)34910670.0595238RNA pol III & RNase P/MRP
0.1041.27E-15SGTC_1350933-0004 26.3 μMChemDiv (Drug-like library)212306170.07812560S ribosome export
0.1024.34E-15SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.12560S ribosome export
0.1025.24E-15SGTC_22067254556 147.0 μMChembridge (Fragment library)43989050.081967260S ribosome export
0.0971.05E-13SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.10769260S ribosome export
0.0961.32E-13SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.081967260S ribosome export
0.0939.94E-13SGTC_490717-0965 32.1 μMChemDiv (Drug-like library)58893490.12560S ribosome export
0.0921.39E-12SGTC_5794491-1009 110.0 μMChemDiv (Drug-like library)12710410.16363660S ribosome export
0.0911.89E-12SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.14516160S ribosome export
0.0895.77E-12SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.13432860S ribosome export
0.0849.05E-11SGTC_32809140961 49.5 μMChembridge (Drug-like library)49012260.11940360S ribosome export
0.0831.89E-10SGTC_31009120536 49.5 μMChembridge (Drug-like library)164211960.0882353

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3451362-0200395 μM0.6578951419823ChemDiv (Drug-like library)264.384684.45100
SGTC_230MPP+141.67 μM0.34285739484Miscellaneous170.230382.95300
SGTC_2181631624054.26 μM0.28571412779990Chembridge (Fragment library)200.233182.50122
SGTC_550767-02856.56 μM0.2631583116166ChemDiv (Drug-like library)325.446086.93500
SGTC_1130099-02212.5 μM0.26315889219ChemDiv (Drug-like library)409.585726.64601
SGTC_6090986-002686.3 μM0.252848610ChemDiv (Drug-like library)302.38965.17901calcium & mitochondrial duress
SGTC_20084012253172 μM0.25603940Chembridge (Fragment library)185.225142.05513
SGTC_15091326-113180 μM0.244444768313ChemDiv (Drug-like library)239.312283.09402
SGTC_8380109-020371.4 μM0.229167759422ChemDiv (Drug-like library)208.258483.75611
SGTC_21945927483104.55 μM0.229167760979Chembridge (Fragment library)201.221241.89112