0121-0052

5-chloro-N-(2,6-dichloro-4-nitrophenyl)-2-hydroxybenzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_365
Screen concentration 13.6 μM
Source ChemDiv (Drug-like library)
PubChem CID 3101739
SMILES C1=CC(=C(C=C1Cl)C(=O)NC2=C(C=C(C=C2Cl)[N+](=O)[O-])Cl)O
Standardized SMILES Oc1ccc(Cl)cc1C(=O)Nc2c(Cl)cc(cc2Cl)[N+](=O)[O-]
Molecular weight 361.5647
ALogP 4.26
H-bond donor count 2
H-bond acceptor count 4
Response signature mitochondrial response to ROS

Pool Growth Kinetics
% growth inhibition (Het. pool) 66.34
% growth inhibition (Hom. pool) 6.59


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3101739
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:-3.87|P-value:5.38E-5|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ARP3(YJR065C)|FD-Score:-4.26|P-value:1.03E-5|Clearance:0||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:CDC39(YCR093W)|FD-Score:-6.43|P-value:6.37E-11|Clearance:0||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CRM1(YGR218W)|FD-Score:5.58|P-value:1.21E-8|Clearance:0.68||SGD DESC:Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin Gene:DIM1(YPL266W)|FD-Score:4.89|P-value:4.93E-7|Clearance:0.01||SGD DESC:Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing Gene:FAS1(YKL182W)|FD-Score:4.04|P-value:2.62E-5|Clearance:0.08||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:FCP1(YMR277W)|FD-Score:3.36|P-value:3.84E-4|Clearance:0.23||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:GLC7(YER133W)|FD-Score:-3.97|P-value:3.65E-5|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:KOG1(YHR186C)|FD-Score:-3.43|P-value:3.06E-4|Clearance:0||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:LCB2(YDR062W)|FD-Score:3.96|P-value:3.69E-5|Clearance:0.1||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:MGE1(YOR232W)|FD-Score:4.88|P-value:5.31E-7|Clearance:0.41||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:NIP1(YMR309C)|FD-Score:-3.15|P-value:8.30E-4|Clearance:0||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:PAB1(YER165W)|FD-Score:-3.23|P-value:6.26E-4|Clearance:0||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:PRE6(YOL038W)|FD-Score:-4.94|P-value:3.82E-7|Clearance:0||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRP24(YMR268C)|FD-Score:-4.47|P-value:3.94E-6|Clearance:0||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RPP1(YHR062C)|FD-Score:-4.11|P-value:1.97E-5|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS31(YLR167W)|FD-Score:-3.21|P-value:6.58E-4|Clearance:0||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:RPS5(YJR123W)|FD-Score:-3.25|P-value:5.68E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:RRB1(YMR131C)|FD-Score:3.81|P-value:7.04E-5|Clearance:0.24||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:RRP7(YCL031C)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.16||SGD DESC:Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress Gene:SNU56(YDR240C)|FD-Score:3.87|P-value:5.52E-5|Clearance:0.06||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex Gene:SPT14(YPL175W)|FD-Score:-3.59|P-value:1.68E-4|Clearance:0||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:SRP101(YDR292C)|FD-Score:5.66|P-value:7.51E-9|Clearance:0.68||SGD DESC:Signal recognition particle (SRP) receptor alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with the beta subunit, Srp102p Gene:TRS31(YDR472W)|FD-Score:-3.61|P-value:1.54E-4|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:USB1(YLR132C)|FD-Score:-3.19|P-value:7.19E-4|Clearance:0||SGD DESC:Putative phosphodiesterase specific for U6 snRNA 3' end modification; trims the 3' poly(u) tract to leave a terminal 3' phosphate; human homolog, hUSB1 (aka C16orf57) produces a 2',3' cyclic phosphate; mutations in hUSB1 are associated with a rare skin condition (OMIM 604173); essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants Gene:UTP4(YDR324C)|FD-Score:-4.12|P-value:1.90E-5|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YDL196W(YDL196W_d)|FD-Score:4.47|P-value:3.85E-6|Clearance:0.43||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YGL074C(YGL074C_d)|FD-Score:3.57|P-value:1.81E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor Gene:YJR012C(YJR012C_p)|FD-Score:3.13|P-value:8.63E-4|Clearance:0.09||SGD DESC:Essential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W Gene:ACT1(YFL039C)|FD-Score:-3.87|P-value:5.38E-5|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ARP3(YJR065C)|FD-Score:-4.26|P-value:1.03E-5|Clearance:0||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:CDC39(YCR093W)|FD-Score:-6.43|P-value:6.37E-11|Clearance:0||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CRM1(YGR218W)|FD-Score:5.58|P-value:1.21E-8|Clearance:0.68||SGD DESC:Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin Gene:DIM1(YPL266W)|FD-Score:4.89|P-value:4.93E-7|Clearance:0.01||SGD DESC:Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing Gene:FAS1(YKL182W)|FD-Score:4.04|P-value:2.62E-5|Clearance:0.08||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:FCP1(YMR277W)|FD-Score:3.36|P-value:3.84E-4|Clearance:0.23||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:GLC7(YER133W)|FD-Score:-3.97|P-value:3.65E-5|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:KOG1(YHR186C)|FD-Score:-3.43|P-value:3.06E-4|Clearance:0||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:LCB2(YDR062W)|FD-Score:3.96|P-value:3.69E-5|Clearance:0.1||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:MGE1(YOR232W)|FD-Score:4.88|P-value:5.31E-7|Clearance:0.41||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:NIP1(YMR309C)|FD-Score:-3.15|P-value:8.30E-4|Clearance:0||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:PAB1(YER165W)|FD-Score:-3.23|P-value:6.26E-4|Clearance:0||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:PRE6(YOL038W)|FD-Score:-4.94|P-value:3.82E-7|Clearance:0||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRP24(YMR268C)|FD-Score:-4.47|P-value:3.94E-6|Clearance:0||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RPP1(YHR062C)|FD-Score:-4.11|P-value:1.97E-5|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS31(YLR167W)|FD-Score:-3.21|P-value:6.58E-4|Clearance:0||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:RPS5(YJR123W)|FD-Score:-3.25|P-value:5.68E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:RRB1(YMR131C)|FD-Score:3.81|P-value:7.04E-5|Clearance:0.24||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:RRP7(YCL031C)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.16||SGD DESC:Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress Gene:SNU56(YDR240C)|FD-Score:3.87|P-value:5.52E-5|Clearance:0.06||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex Gene:SPT14(YPL175W)|FD-Score:-3.59|P-value:1.68E-4|Clearance:0||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:SRP101(YDR292C)|FD-Score:5.66|P-value:7.51E-9|Clearance:0.68||SGD DESC:Signal recognition particle (SRP) receptor alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with the beta subunit, Srp102p Gene:TRS31(YDR472W)|FD-Score:-3.61|P-value:1.54E-4|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:USB1(YLR132C)|FD-Score:-3.19|P-value:7.19E-4|Clearance:0||SGD DESC:Putative phosphodiesterase specific for U6 snRNA 3' end modification; trims the 3' poly(u) tract to leave a terminal 3' phosphate; human homolog, hUSB1 (aka C16orf57) produces a 2',3' cyclic phosphate; mutations in hUSB1 are associated with a rare skin condition (OMIM 604173); essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants Gene:UTP4(YDR324C)|FD-Score:-4.12|P-value:1.90E-5|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YDL196W(YDL196W_d)|FD-Score:4.47|P-value:3.85E-6|Clearance:0.43||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YGL074C(YGL074C_d)|FD-Score:3.57|P-value:1.81E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor Gene:YJR012C(YJR012C_p)|FD-Score:3.13|P-value:8.63E-4|Clearance:0.09||SGD DESC:Essential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3101739
Download HOP data (tab-delimited text)  (excel)
Gene:ADE3(YGR204W)|FD-Score:-3.74|P-value:9.21E-5||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:AIF1(YNR074C)|FD-Score:-3.35|P-value:4.11E-4||SGD DESC:Mitochondrial cell death effector that translocates to the nucleus in response to apoptotic stimuli, homolog of mammalian Apoptosis-Inducing Factor, putative reductase Gene:ALT1(YLR089C)|FD-Score:-3.1|P-value:9.81E-4||SGD DESC:Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthetic and catabolic processes; expression is induced in the presence of alanine; repression is mediated by Nrg1p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; paralogous gene ALT2 is catalytically inactive Gene:ARL1(YBR164C)|FD-Score:4.67|P-value:1.51E-6||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARO2(YGL148W)|FD-Score:5.21|P-value:9.39E-8||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATG18(YFR021W)|FD-Score:5.93|P-value:1.51E-9||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:ATP25(YMR098C)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene Gene:ATP3(YBR039W)|FD-Score:6.94|P-value:1.98E-12||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:ATP7(YKL016C)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BAS1(YKR099W)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes Gene:BSP1(YPR171W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:CCM1(YGR150C)|FD-Score:7.01|P-value:1.21E-12||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CHO1(YER026C)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline Gene:CIN4(YMR138W)|FD-Score:5.08|P-value:1.92E-7||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:CKI1(YLR133W)|FD-Score:-4.73|P-value:1.12E-6||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:CLC1(YGR167W)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CNM67(YNL225C)|FD-Score:-3.85|P-value:5.87E-5||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COG8(YML071C)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ8(YGL119W)|FD-Score:4.99|P-value:3.07E-7||SGD DESC:Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication Gene:COX6(YHR051W)|FD-Score:4.59|P-value:2.19E-6||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:CRD1(YDL142C)|FD-Score:3.75|P-value:8.86E-5||SGD DESC:Cardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function; also required for normal vacuolar ion homeostasis Gene:CSG2(YBR036C)|FD-Score:-4.54|P-value:2.80E-6||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CTK1(YKL139W)|FD-Score:7.63|P-value:1.20E-14||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:EAP1(YKL204W)|FD-Score:-3.84|P-value:6.15E-5||SGD DESC:eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade Gene:ECI1(YLR284C)|FD-Score:-5.02|P-value:2.53E-7||SGD DESC:Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced Gene:ECM33(YBR078W)|FD-Score:8.95|P-value:1.75E-19||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ELP2(YGR200C)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:ELP6(YMR312W)|FD-Score:-4.64|P-value:1.74E-6||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ERD1(YDR414C)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:ERG28(YER044C)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p Gene:ERV14(YGL054C)|FD-Score:5.2|P-value:1.01E-7||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FAB1(YFR019W)|FD-Score:4.03|P-value:2.77E-5||SGD DESC:1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis Gene:FCJ1(YKR016W)|FD-Score:4.38|P-value:5.81E-6||SGD DESC:Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane Gene:FRA2(YGL220W)|FD-Score:-3.8|P-value:7.16E-5||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:FTR1(YER145C)|FD-Score:-7|P-value:1.27E-12||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:FZO1(YBR179C)|FD-Score:5.7|P-value:6.11E-9||SGD DESC:Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system Gene:GCN2(YDR283C)|FD-Score:3.24|P-value:6.04E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GEP4(YHR100C)|FD-Score:4.66|P-value:1.61E-6||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GLO3(YER122C)|FD-Score:4.28|P-value:9.29E-6||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p Gene:GTF1(YGR102C)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria Gene:HFI1(YPL254W)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HFM1(YGL251C)|FD-Score:-3.29|P-value:5.05E-4||SGD DESC:Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity Gene:HST1(YOL068C)|FD-Score:-5.43|P-value:2.88E-8||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:IBA57(YJR122W)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:ICL2(YPR006C)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:2-methylisocitrate lyase of the mitochondrial matrix, functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol Gene:IRC3(YDR332W)|FD-Score:4.46|P-value:4.02E-6||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:IRS4(YKR019C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:IST2(YBR086C)|FD-Score:-3.37|P-value:3.77E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process Gene:KAP120(YPL125W)|FD-Score:5.1|P-value:1.69E-7||SGD DESC:Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p Gene:LGE1(YPL055C)|FD-Score:-3.36|P-value:3.95E-4||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LIA1(YJR070C)|FD-Score:6.12|P-value:4.74E-10||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:MAK3(YPR051W)|FD-Score:4.48|P-value:3.71E-6||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:MAL32(YBR299W)|FD-Score:-5.07|P-value:2.04E-7||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MEF2(YJL102W)|FD-Score:-3.19|P-value:7.20E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MIP1(YOR330C)|FD-Score:8.82|P-value:5.61E-19||SGD DESC:Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit Gene:MMT1(YMR177W)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p Gene:MPA43(YNL249C)|FD-Score:3.92|P-value:4.46E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MRM1(YOR201C)|FD-Score:3.89|P-value:5.01E-5||SGD DESC:Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA Gene:MRP1(YDR347W)|FD-Score:5.53|P-value:1.62E-8||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein Gene:MRPL20(YKR085C)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL33(YMR286W)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL7(YDR237W)|FD-Score:3.72|P-value:9.88E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:MRPL9(YGR220C)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS16(YPL013C)|FD-Score:5.34|P-value:4.58E-8||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPS28(YDR337W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPS5(YBR251W)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSR1(YHR091C)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Mitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6 Gene:MST1(YKL194C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:MTG2(YHR168W)|FD-Score:4.1|P-value:2.04E-5||SGD DESC:Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly Gene:NAM2(YLR382C)|FD-Score:5.3|P-value:5.79E-8||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:NDE2(YDL085W)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain Gene:NTH2(YBR001C)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:OAC1(YKL120W)|FD-Score:-6.73|P-value:8.40E-12||SGD DESC:Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family Gene:PEP12(YOR036W)|FD-Score:7.2|P-value:2.91E-13||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP3(YLR148W)|FD-Score:3.82|P-value:6.72E-5||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEP7(YDR323C)|FD-Score:16.5|P-value:3.39E-61||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PET100(YDR079W)|FD-Score:4.62|P-value:1.91E-6||SGD DESC:Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme Gene:PET112(YBL080C)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PET123(YOR158W)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:PET130(YJL023C)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PET309(YLR067C)|FD-Score:3.31|P-value:4.58E-4||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PFK2(YMR205C)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:POR2(YIL114C)|FD-Score:-4.17|P-value:1.51E-5||SGD DESC:Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication Gene:PPQ1(YPL179W)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Putative protein serine/threonine phosphatase; null mutation enhances efficiency of translational suppressors Gene:PPT2(YPL148C)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PUS2(YGL063W)|FD-Score:-4.19|P-value:1.37E-5||SGD DESC:Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification Gene:RCK2(YLR248W)|FD-Score:3.72|P-value:9.83E-5||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RHR2(YIL053W)|FD-Score:-4.96|P-value:3.46E-7||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RIP1(YEL024W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly Gene:RMD9(YGL107C)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes Gene:RML2(YEL050C)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPL12B(YDR418W)|FD-Score:-3.28|P-value:5.12E-4||SGD DESC:Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication Gene:RPO41(YFL036W)|FD-Score:20.1|P-value:3.83E-90||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RPS11B(YBR048W)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication Gene:RPS27A(YKL156W)|FD-Score:5.07|P-value:1.97E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27A has a paralog, RPS27B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RRG9(YNL213C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RSM19(YNR037C)|FD-Score:4.72|P-value:1.17E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein Gene:RSM22(YKL155C)|FD-Score:5.6|P-value:1.10E-8||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:RSM23(YGL129C)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase Mca1p Gene:RSM25(YIL093C)|FD-Score:3.73|P-value:9.45E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SAC6(YDR129C)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SEC66(YBR171W)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SGN1(YIR001C)|FD-Score:3.73|P-value:9.67E-5||SGD DESC:Cytoplasmic RNA-binding protein, contains an RNA recognition motif (RRM); may have a role in mRNA translation, as suggested by genetic interactions with genes encoding proteins involved in translational initiation Gene:SKG6(YHR149C)|FD-Score:-3.21|P-value:6.75E-4||SGD DESC:Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p Gene:SLA1(YBL007C)|FD-Score:4.05|P-value:2.56E-5||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SMM1(YNR015W)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Dihydrouridine synthase, member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs Gene:SNF7(YLR025W)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNX4(YJL036W)|FD-Score:-6.08|P-value:5.98E-10||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPC1(YJR010C-A)|FD-Score:3.89|P-value:4.93E-5||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:TLG2(YOL018C)|FD-Score:6.81|P-value:4.94E-12||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TPS2(YDR074W)|FD-Score:4.62|P-value:1.88E-6||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:TRP1(YDR007W)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:7.34|P-value:1.08E-13||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TUP1(YCR084C)|FD-Score:-3.3|P-value:4.76E-4||SGD DESC:General repressor of transcription, forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes Gene:UBI4(YLL039C)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress Gene:UBP12(YJL197W)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm Gene:VAC14(YLR386W)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VID22(YLR373C)|FD-Score:-3.97|P-value:3.57E-5||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:VPS25(YJR102C)|FD-Score:4.31|P-value:8.11E-6||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS27(YNR006W)|FD-Score:4.08|P-value:2.23E-5||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS4(YPR173C)|FD-Score:8.06|P-value:3.93E-16||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:VPS45(YGL095C)|FD-Score:7.65|P-value:1.01E-14||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS52(YDR484W)|FD-Score:3.09|P-value:0.00100||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:VPS70(YJR126C)|FD-Score:4.39|P-value:5.72E-6||SGD DESC:Protein of unknown function involved in vacuolar protein sorting Gene:VPS74(YDR372C)|FD-Score:4.54|P-value:2.77E-6||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YAR1(YPL239W)|FD-Score:-4.14|P-value:1.76E-5||SGD DESC:Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock Gene:YBR141C(YBR141C_p)|FD-Score:-4.06|P-value:2.40E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YCR075W-A(YCR075W-A_p)|FD-Score:-3.2|P-value:6.98E-4||SGD DESC:Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication Gene:YDL009C(YDL009C_p)|FD-Score:-4.61|P-value:2.01E-6||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:4.1|P-value:2.11E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR115W(YDR115W_p)|FD-Score:4.93|P-value:4.16E-7||SGD DESC:Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress Gene:YDR230W(YDR230W_d)|FD-Score:3.11|P-value:9.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20 Gene:YDR338C(YDR338C_p)|FD-Score:5.34|P-value:4.72E-8||SGD DESC:Putative protein of unknown function, member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily Gene:YDR445C(YDR445C_d)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER121W(YER121W_p)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy Gene:YFL019C(YFL019C_d)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YGR021W(YGR021W_p)|FD-Score:-7.9|P-value:1.35E-15||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YHB1(YGR234W)|FD-Score:-3.39|P-value:3.55E-4||SGD DESC:Nitric oxide oxidoreductase; flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:YIL012W(YIL012W_d)|FD-Score:5.1|P-value:1.72E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL032C(YIL032C_d)|FD-Score:-4.13|P-value:1.80E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL022W(YJL022W_d)|FD-Score:8.89|P-value:3.07E-19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Gene:YKE2(YLR200W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YKL068W-A(YKL068W-A_p)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YLR217W(YLR217W_d)|FD-Score:5.5|P-value:1.89E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CPR6 Gene:YMR119W-A(YMR119W-A_d)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR245W(YMR245W_d)|FD-Score:3.2|P-value:6.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR272W-B(YMR272W-B_p)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YNL184C(YNL184C_p)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOL106W(YOL106W_d)|FD-Score:5.16|P-value:1.25E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR139C(YOR139C_d)|FD-Score:-3.47|P-value:2.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YOR200W(YOR200W_d)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YPK1(YKL126W)|FD-Score:5.94|P-value:1.42E-9||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:ZAP1(YJL056C)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:ADE3(YGR204W)|FD-Score:-3.74|P-value:9.21E-5||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:AIF1(YNR074C)|FD-Score:-3.35|P-value:4.11E-4||SGD DESC:Mitochondrial cell death effector that translocates to the nucleus in response to apoptotic stimuli, homolog of mammalian Apoptosis-Inducing Factor, putative reductase Gene:ALT1(YLR089C)|FD-Score:-3.1|P-value:9.81E-4||SGD DESC:Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthetic and catabolic processes; expression is induced in the presence of alanine; repression is mediated by Nrg1p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; paralogous gene ALT2 is catalytically inactive Gene:ARL1(YBR164C)|FD-Score:4.67|P-value:1.51E-6||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARO2(YGL148W)|FD-Score:5.21|P-value:9.39E-8||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATG18(YFR021W)|FD-Score:5.93|P-value:1.51E-9||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:ATP25(YMR098C)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene Gene:ATP3(YBR039W)|FD-Score:6.94|P-value:1.98E-12||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:ATP7(YKL016C)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BAS1(YKR099W)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes Gene:BSP1(YPR171W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:CCM1(YGR150C)|FD-Score:7.01|P-value:1.21E-12||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CHO1(YER026C)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline Gene:CIN4(YMR138W)|FD-Score:5.08|P-value:1.92E-7||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:CKI1(YLR133W)|FD-Score:-4.73|P-value:1.12E-6||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:CLC1(YGR167W)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CNM67(YNL225C)|FD-Score:-3.85|P-value:5.87E-5||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COG8(YML071C)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ8(YGL119W)|FD-Score:4.99|P-value:3.07E-7||SGD DESC:Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication Gene:COX6(YHR051W)|FD-Score:4.59|P-value:2.19E-6||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:CRD1(YDL142C)|FD-Score:3.75|P-value:8.86E-5||SGD DESC:Cardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function; also required for normal vacuolar ion homeostasis Gene:CSG2(YBR036C)|FD-Score:-4.54|P-value:2.80E-6||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CTK1(YKL139W)|FD-Score:7.63|P-value:1.20E-14||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:EAP1(YKL204W)|FD-Score:-3.84|P-value:6.15E-5||SGD DESC:eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade Gene:ECI1(YLR284C)|FD-Score:-5.02|P-value:2.53E-7||SGD DESC:Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced Gene:ECM33(YBR078W)|FD-Score:8.95|P-value:1.75E-19||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ELP2(YGR200C)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:ELP6(YMR312W)|FD-Score:-4.64|P-value:1.74E-6||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ERD1(YDR414C)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:ERG28(YER044C)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p Gene:ERV14(YGL054C)|FD-Score:5.2|P-value:1.01E-7||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FAB1(YFR019W)|FD-Score:4.03|P-value:2.77E-5||SGD DESC:1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis Gene:FCJ1(YKR016W)|FD-Score:4.38|P-value:5.81E-6||SGD DESC:Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane Gene:FRA2(YGL220W)|FD-Score:-3.8|P-value:7.16E-5||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:FTR1(YER145C)|FD-Score:-7|P-value:1.27E-12||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:FZO1(YBR179C)|FD-Score:5.7|P-value:6.11E-9||SGD DESC:Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system Gene:GCN2(YDR283C)|FD-Score:3.24|P-value:6.04E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GEP4(YHR100C)|FD-Score:4.66|P-value:1.61E-6||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GLO3(YER122C)|FD-Score:4.28|P-value:9.29E-6||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p Gene:GTF1(YGR102C)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria Gene:HFI1(YPL254W)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HFM1(YGL251C)|FD-Score:-3.29|P-value:5.05E-4||SGD DESC:Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity Gene:HST1(YOL068C)|FD-Score:-5.43|P-value:2.88E-8||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:IBA57(YJR122W)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:ICL2(YPR006C)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:2-methylisocitrate lyase of the mitochondrial matrix, functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol Gene:IRC3(YDR332W)|FD-Score:4.46|P-value:4.02E-6||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:IRS4(YKR019C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:IST2(YBR086C)|FD-Score:-3.37|P-value:3.77E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process Gene:KAP120(YPL125W)|FD-Score:5.1|P-value:1.69E-7||SGD DESC:Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p Gene:LGE1(YPL055C)|FD-Score:-3.36|P-value:3.95E-4||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LIA1(YJR070C)|FD-Score:6.12|P-value:4.74E-10||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:MAK3(YPR051W)|FD-Score:4.48|P-value:3.71E-6||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:MAL32(YBR299W)|FD-Score:-5.07|P-value:2.04E-7||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MEF2(YJL102W)|FD-Score:-3.19|P-value:7.20E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MIP1(YOR330C)|FD-Score:8.82|P-value:5.61E-19||SGD DESC:Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit Gene:MMT1(YMR177W)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p Gene:MPA43(YNL249C)|FD-Score:3.92|P-value:4.46E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MRM1(YOR201C)|FD-Score:3.89|P-value:5.01E-5||SGD DESC:Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA Gene:MRP1(YDR347W)|FD-Score:5.53|P-value:1.62E-8||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein Gene:MRPL20(YKR085C)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL33(YMR286W)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL7(YDR237W)|FD-Score:3.72|P-value:9.88E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:MRPL9(YGR220C)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS16(YPL013C)|FD-Score:5.34|P-value:4.58E-8||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPS28(YDR337W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPS5(YBR251W)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSR1(YHR091C)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Mitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6 Gene:MST1(YKL194C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:MTG2(YHR168W)|FD-Score:4.1|P-value:2.04E-5||SGD DESC:Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly Gene:NAM2(YLR382C)|FD-Score:5.3|P-value:5.79E-8||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:NDE2(YDL085W)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain Gene:NTH2(YBR001C)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:OAC1(YKL120W)|FD-Score:-6.73|P-value:8.40E-12||SGD DESC:Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family Gene:PEP12(YOR036W)|FD-Score:7.2|P-value:2.91E-13||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP3(YLR148W)|FD-Score:3.82|P-value:6.72E-5||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEP7(YDR323C)|FD-Score:16.5|P-value:3.39E-61||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PET100(YDR079W)|FD-Score:4.62|P-value:1.91E-6||SGD DESC:Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme Gene:PET112(YBL080C)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PET123(YOR158W)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:PET130(YJL023C)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PET309(YLR067C)|FD-Score:3.31|P-value:4.58E-4||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PFK2(YMR205C)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:POR2(YIL114C)|FD-Score:-4.17|P-value:1.51E-5||SGD DESC:Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication Gene:PPQ1(YPL179W)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Putative protein serine/threonine phosphatase; null mutation enhances efficiency of translational suppressors Gene:PPT2(YPL148C)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PUS2(YGL063W)|FD-Score:-4.19|P-value:1.37E-5||SGD DESC:Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification Gene:RCK2(YLR248W)|FD-Score:3.72|P-value:9.83E-5||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RHR2(YIL053W)|FD-Score:-4.96|P-value:3.46E-7||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RIP1(YEL024W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly Gene:RMD9(YGL107C)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes Gene:RML2(YEL050C)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPL12B(YDR418W)|FD-Score:-3.28|P-value:5.12E-4||SGD DESC:Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication Gene:RPO41(YFL036W)|FD-Score:20.1|P-value:3.83E-90||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RPS11B(YBR048W)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication Gene:RPS27A(YKL156W)|FD-Score:5.07|P-value:1.97E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27A has a paralog, RPS27B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RRG9(YNL213C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RSM19(YNR037C)|FD-Score:4.72|P-value:1.17E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein Gene:RSM22(YKL155C)|FD-Score:5.6|P-value:1.10E-8||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:RSM23(YGL129C)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase Mca1p Gene:RSM25(YIL093C)|FD-Score:3.73|P-value:9.45E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SAC6(YDR129C)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SEC66(YBR171W)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SGN1(YIR001C)|FD-Score:3.73|P-value:9.67E-5||SGD DESC:Cytoplasmic RNA-binding protein, contains an RNA recognition motif (RRM); may have a role in mRNA translation, as suggested by genetic interactions with genes encoding proteins involved in translational initiation Gene:SKG6(YHR149C)|FD-Score:-3.21|P-value:6.75E-4||SGD DESC:Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p Gene:SLA1(YBL007C)|FD-Score:4.05|P-value:2.56E-5||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SMM1(YNR015W)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Dihydrouridine synthase, member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs Gene:SNF7(YLR025W)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNX4(YJL036W)|FD-Score:-6.08|P-value:5.98E-10||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPC1(YJR010C-A)|FD-Score:3.89|P-value:4.93E-5||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:TLG2(YOL018C)|FD-Score:6.81|P-value:4.94E-12||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TPS2(YDR074W)|FD-Score:4.62|P-value:1.88E-6||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:TRP1(YDR007W)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:7.34|P-value:1.08E-13||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TUP1(YCR084C)|FD-Score:-3.3|P-value:4.76E-4||SGD DESC:General repressor of transcription, forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes Gene:UBI4(YLL039C)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress Gene:UBP12(YJL197W)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm Gene:VAC14(YLR386W)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VID22(YLR373C)|FD-Score:-3.97|P-value:3.57E-5||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:VPS25(YJR102C)|FD-Score:4.31|P-value:8.11E-6||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS27(YNR006W)|FD-Score:4.08|P-value:2.23E-5||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS4(YPR173C)|FD-Score:8.06|P-value:3.93E-16||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:VPS45(YGL095C)|FD-Score:7.65|P-value:1.01E-14||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS52(YDR484W)|FD-Score:3.09|P-value:0.00100||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:VPS70(YJR126C)|FD-Score:4.39|P-value:5.72E-6||SGD DESC:Protein of unknown function involved in vacuolar protein sorting Gene:VPS74(YDR372C)|FD-Score:4.54|P-value:2.77E-6||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YAR1(YPL239W)|FD-Score:-4.14|P-value:1.76E-5||SGD DESC:Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock Gene:YBR141C(YBR141C_p)|FD-Score:-4.06|P-value:2.40E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YCR075W-A(YCR075W-A_p)|FD-Score:-3.2|P-value:6.98E-4||SGD DESC:Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication Gene:YDL009C(YDL009C_p)|FD-Score:-4.61|P-value:2.01E-6||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:4.1|P-value:2.11E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR115W(YDR115W_p)|FD-Score:4.93|P-value:4.16E-7||SGD DESC:Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress Gene:YDR230W(YDR230W_d)|FD-Score:3.11|P-value:9.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20 Gene:YDR338C(YDR338C_p)|FD-Score:5.34|P-value:4.72E-8||SGD DESC:Putative protein of unknown function, member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily Gene:YDR445C(YDR445C_d)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER121W(YER121W_p)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy Gene:YFL019C(YFL019C_d)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YGR021W(YGR021W_p)|FD-Score:-7.9|P-value:1.35E-15||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YHB1(YGR234W)|FD-Score:-3.39|P-value:3.55E-4||SGD DESC:Nitric oxide oxidoreductase; flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:YIL012W(YIL012W_d)|FD-Score:5.1|P-value:1.72E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL032C(YIL032C_d)|FD-Score:-4.13|P-value:1.80E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL022W(YJL022W_d)|FD-Score:8.89|P-value:3.07E-19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Gene:YKE2(YLR200W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YKL068W-A(YKL068W-A_p)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YLR217W(YLR217W_d)|FD-Score:5.5|P-value:1.89E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CPR6 Gene:YMR119W-A(YMR119W-A_d)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR245W(YMR245W_d)|FD-Score:3.2|P-value:6.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR272W-B(YMR272W-B_p)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YNL184C(YNL184C_p)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOL106W(YOL106W_d)|FD-Score:5.16|P-value:1.25E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR139C(YOR139C_d)|FD-Score:-3.47|P-value:2.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YOR200W(YOR200W_d)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YPK1(YKL126W)|FD-Score:5.94|P-value:1.42E-9||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:ZAP1(YJL056C)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YDR292C5.667.51E-90.68SRP101Signal recognition particle (SRP) receptor alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with the beta subunit, Srp102p
YGR218W5.581.21E-80.68CRM1Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin
YPL266W4.894.93E-70.01DIM1Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing
YOR232W4.885.31E-70.41MGE1Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE
YDL196W_d4.473.85E-60.43YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YKL182W4.042.62E-50.08FAS1Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
YDR062W3.963.69E-50.10LCB2Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
YDR240C3.875.52E-50.06SNU56Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex
YMR131C3.817.04E-50.24RRB1Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p
YGL074C_d3.571.81E-40.04YGL074C_dDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor
YCL031C3.532.09E-40.16RRP7Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress
YMR277W3.363.84E-40.23FCP1Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)
YJR012C_p3.138.63E-40.09YJR012C_pEssential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W
YLR355C3.050.001150.04ILV5Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
YLL008W3.010.001300.04DRS1Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YFL036W20.103.83E-90RPO41Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
YDR323C16.503.39E-61PEP7Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
YBR078W8.951.75E-19ECM33GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p
YJL022W_d8.893.07E-19YJL022W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130
YOR330C8.825.61E-19MIP1Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
YPR173C8.063.93E-16VPS4AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism
YGL095C7.651.01E-14VPS45Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
YKL139W7.631.20E-14CTK1Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13
YKL211C7.341.08E-13TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YOR036W7.202.91E-13PEP12Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
YGR150C7.011.21E-12CCM1Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport
YBR039W6.941.98E-12ATP3Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis
YOL018C6.814.94E-12TLG2Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16)
YJR070C6.124.74E-10LIA1Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress
YKL126W5.941.42E-9YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication

GO enrichment analysis for SGTC_365
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3359.97E-155SGTC_275trichlorophene 5.7 μMMiscellaneous626160.265306mitochondrial stress
0.2783.25E-105SGTC_9083448-7491 47.7 μMChemDiv (Drug-like library)12030310.0793651mitochondrial stress
0.2261.84E-69SGTC_1190121-0043 24.8 μMChemDiv (Drug-like library)10923780.315789mitochondrial stress
0.2201.35E-65SGTC_748act1.228 77.9 μMChemDiv (Drug-like library)44215050.150943redox potentiating
0.2142.07E-62SGTC_700141-0289 43.3 μMChemDiv (Drug-like library)3524080.333333mitochondrial stress
0.2111.50E-60SGTC_12400356-0016 42.1 μMChemDiv (Drug-like library)43056130.0972222mitochondrial response to ROS
0.2074.13E-58SGTC_3861509-0011 3.1 μMChemDiv (Drug-like library)31072800.220339
0.2075.64E-58SGTC_2361509-0011 5.3 μMChemDiv (Drug-like library)31072800.220339
0.1882.62E-48SGTC_1593justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one 54.9 μMTimTec (Pure natural product library)4428820.0649351redox potentiating
0.1823.05E-45SGTC_11881403-0014 4.6 μMChemDiv (Drug-like library)96148670.0746269mitochondrial stress
0.1701.12E-39SGTC_100279-0212 42.6 μMChemDiv (Drug-like library)398510.125TRP & mitochondrial translation
0.1651.75E-37SGTC_1731273-0061 2.7 μMChemDiv (Drug-like library)28474140.0434783mitochondrial stress
0.1653.39E-37SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous12732100.0645161TRP & mitochondrial translation
0.1582.71E-34SGTC_1635st007035 42.4 μMTimTec (Natural product derivative library)37164030.0481928
0.1503.54E-31SGTC_6704204-0025 119.0 μMChemDiv (Drug-like library)7190390.147059copper-dependent oxidative stress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_7600568-072582.9 μM0.5416673916171ChemDiv (Drug-like library)380.010345.16613
SGTC_7251497-096319 μM0.4897963549977ChemDiv (Drug-like library)356.117783.73215copper-dependent oxidative stress
SGTC_1240136-02177.12 μM0.4655173976905ChemDiv (Drug-like library)476.223245.16417mitochondrial stress
SGTC_2230bromochlorosalicylanilide954.5 nM0.4489877254Miscellaneous326.573063.78522
SGTC_9252064-127259.6 μM0.3770496815817ChemDiv (Drug-like library)333.726481.44825
SGTC_3700180-034538.7 μM0.3529413989527ChemDiv (Drug-like library)385.457245.69422DNA intercalators
SGTC_6260214-000933 μM0.3518523437874ChemDiv (Drug-like library)332.225222.29717copper-dependent oxidative stress
SGTC_11971601-0112267 μM0.357350313ChemDiv (Drug-like library)299.281422.31625
SGTC_2449581298245.77 μM0.339286776185Miscellaneous292.6895033.78214
SGTC_1301naphtanilide LB4.81 μM0.33898367238ChemDiv (Drug-like library)336.771724.5332heme requiring