0566-0070

[(E)-3-oxo-3-phenylprop-1-enyl] thiocyanate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_37
Screen concentration 1.6 μM
Source ChemDiv (Drug-like library)
PubChem CID 1626686
SMILES C1=CC=C(C=C1)C(=O)C=CSC#N
Standardized SMILES O=C(C=CSC#N)c1ccccc1
Molecular weight 189.2337
ALogP 2.13
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 44.22
% growth inhibition (Hom. pool) 19.58


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1626686
Download HIP data (tab-delimited text)  (excel)
Gene:DRS1(YLL008W)|FD-Score:-3.13|P-value:8.72E-4|Clearance:0||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:FCP1(YMR277W)|FD-Score:-3.29|P-value:5.07E-4|Clearance:0||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:LCB2(YDR062W)|FD-Score:3.52|P-value:2.18E-4|Clearance:0.14||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:NIP7(YPL211W)|FD-Score:-3.39|P-value:3.45E-4|Clearance:0||SGD DESC:Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p Gene:NOP4(YPL043W)|FD-Score:-4.26|P-value:1.01E-5|Clearance:0||SGD DESC:Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) Gene:NOP56(YLR197W)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.17||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NUP145(YGL092W)|FD-Score:3.35|P-value:4.01E-4|Clearance:0.17||SGD DESC:Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 Gene:PRP3(YDR473C)|FD-Score:4.18|P-value:1.43E-5|Clearance:0.08||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:PRP38(YGR075C)|FD-Score:3.12|P-value:9.08E-4|Clearance:0.03||SGD DESC:Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly Gene:PRP9(YDL030W)|FD-Score:3.38|P-value:3.66E-4|Clearance:0.03||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex Gene:RPS31(YLR167W)|FD-Score:4.3|P-value:8.59E-6|Clearance:0.11||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:RRP42(YDL111C)|FD-Score:3.19|P-value:7.19E-4|Clearance:0.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:RSE1(YML049C)|FD-Score:-3.21|P-value:6.59E-4|Clearance:0||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:SEC65(YML105C)|FD-Score:3.38|P-value:3.61E-4|Clearance:0||SGD DESC:Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19 Gene:SPB4(YFL002C)|FD-Score:4.1|P-value:2.03E-5|Clearance:0.42||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:TFA1(YKL028W)|FD-Score:-4.2|P-value:1.36E-5|Clearance:0||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TIF6(YPR016C)|FD-Score:4.83|P-value:6.76E-7|Clearance:0.53||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:YDL196W(YDL196W_d)|FD-Score:-3.39|P-value:3.55E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:DRS1(YLL008W)|FD-Score:-3.13|P-value:8.72E-4|Clearance:0||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:FCP1(YMR277W)|FD-Score:-3.29|P-value:5.07E-4|Clearance:0||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:LCB2(YDR062W)|FD-Score:3.52|P-value:2.18E-4|Clearance:0.14||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:NIP7(YPL211W)|FD-Score:-3.39|P-value:3.45E-4|Clearance:0||SGD DESC:Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p Gene:NOP4(YPL043W)|FD-Score:-4.26|P-value:1.01E-5|Clearance:0||SGD DESC:Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) Gene:NOP56(YLR197W)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.17||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NUP145(YGL092W)|FD-Score:3.35|P-value:4.01E-4|Clearance:0.17||SGD DESC:Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 Gene:PRP3(YDR473C)|FD-Score:4.18|P-value:1.43E-5|Clearance:0.08||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:PRP38(YGR075C)|FD-Score:3.12|P-value:9.08E-4|Clearance:0.03||SGD DESC:Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly Gene:PRP9(YDL030W)|FD-Score:3.38|P-value:3.66E-4|Clearance:0.03||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex Gene:RPS31(YLR167W)|FD-Score:4.3|P-value:8.59E-6|Clearance:0.11||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:RRP42(YDL111C)|FD-Score:3.19|P-value:7.19E-4|Clearance:0.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:RSE1(YML049C)|FD-Score:-3.21|P-value:6.59E-4|Clearance:0||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:SEC65(YML105C)|FD-Score:3.38|P-value:3.61E-4|Clearance:0||SGD DESC:Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19 Gene:SPB4(YFL002C)|FD-Score:4.1|P-value:2.03E-5|Clearance:0.42||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:TFA1(YKL028W)|FD-Score:-4.2|P-value:1.36E-5|Clearance:0||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TIF6(YPR016C)|FD-Score:4.83|P-value:6.76E-7|Clearance:0.53||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:YDL196W(YDL196W_d)|FD-Score:-3.39|P-value:3.55E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1626686
Download HOP data (tab-delimited text)  (excel)
Gene:ADH6(YMR318C)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:AIM9(YER080W)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:AMS1(YGL156W)|FD-Score:3.87|P-value:5.34E-5||SGD DESC:Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway Gene:ARC18(YLR370C)|FD-Score:-3.13|P-value:8.74E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARG81(YML099C)|FD-Score:-6.15|P-value:3.91E-10||SGD DESC:Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p Gene:ATG19(YOL082W)|FD-Score:-4.43|P-value:4.77E-6||SGD DESC:Receptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathway; delivers cargo proteins aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p) to the phagophore assembly site for packaging into Cvt vesicles Gene:ATP7(YKL016C)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BIK1(YCL029C)|FD-Score:5.79|P-value:3.62E-9||SGD DESC:Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 Gene:BLM10(YFL007W)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Proteasome activator; binds the core proteasome and stimulates proteasome-mediated protein degradation by inducing gate opening; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200 Gene:BUD19(YJL188C_d)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUD28(YLR062C_d)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CCZ1(YBR131W)|FD-Score:3.74|P-value:9.34E-5||SGD DESC:Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway Gene:CRT10(YOL063C)|FD-Score:-3.12|P-value:9.17E-4||SGD DESC:Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat Gene:CSR2(YPR030W)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Nuclear ubiquitin protein ligase binding protein; may regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p; CSR2 has a paralog, ECM21, that arose from the whole genome duplication Gene:DID4(YKL002W)|FD-Score:5.89|P-value:1.90E-9||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:DOC1(YGL240W)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain Gene:DPH5(YLR172C)|FD-Score:4.61|P-value:1.99E-6||SGD DESC:Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm Gene:ELM1(YKL048C)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:ELP2(YGR200C)|FD-Score:4.72|P-value:1.17E-6||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:ELP3(YPL086C)|FD-Score:4.96|P-value:3.61E-7||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin Gene:ELP6(YMR312W)|FD-Score:3.98|P-value:3.38E-5||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:EMI2(YDR516C)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:EXG2(YDR261C)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:FIN1(YDR130C)|FD-Score:-4.45|P-value:4.34E-6||SGD DESC:Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress Gene:GLE2(YER107C)|FD-Score:5|P-value:2.84E-7||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:HOM6(YJR139C)|FD-Score:8.76|P-value:1.01E-18||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HSC82(YMR186W)|FD-Score:3.13|P-value:8.70E-4||SGD DESC:Cytoplasmic chaperone of the Hsp90 family; redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock; contains two acid-rich unstructured regions that promote the solubility of chaperone-substrate complexes; HSC82 has a paralog, HSP82, that arose from the whole genome duplication Gene:IKI1(YHR187W)|FD-Score:4.55|P-value:2.63E-6||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:IKI3(YLR384C)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) Gene:IRE1(YHR079C)|FD-Score:-3.17|P-value:7.66E-4||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:IZH4(YOL101C)|FD-Score:-5.25|P-value:7.49E-8||SGD DESC:Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress Gene:KGD1(YIL125W)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA Gene:LCB5(YLR260W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:LCL2(YLR104W_p)|FD-Score:-3.9|P-value:4.85E-5||SGD DESC:Putative protein of unknown function; mutant is deficient in amounts of cell wall mannosylphosphate and has long chronological lifespan; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:LDB7(YBL006C)|FD-Score:-5.42|P-value:2.99E-8||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LOC1(YFR001W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:LOS1(YKL205W)|FD-Score:3.12|P-value:9.10E-4||SGD DESC:Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:LPX1(YOR084W)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Oleic acid-inducible, peroxisomal matrix localized lipase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; peroxisomal import is dependent on the PTS1 receptor, Pex5p and on self-interaction Gene:LSM1(YJL124C)|FD-Score:4|P-value:3.23E-5||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:LSM7(YNL147W)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:MGA1(YGR249W)|FD-Score:4.04|P-value:2.72E-5||SGD DESC:Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants Gene:MLP1(YKR095W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs Gene:MOG1(YJR074W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:NBA1(YOL070C)|FD-Score:-3.22|P-value:6.37E-4||SGD DESC:Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate Gene:NUT1(YGL151W)|FD-Score:5.54|P-value:1.49E-8||SGD DESC:Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription Gene:OPI9(YLR338W_d)|FD-Score:-6.55|P-value:2.79E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:OSH3(YHR073W)|FD-Score:-4.25|P-value:1.09E-5||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PEP7(YDR323C)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PPT2(YPL148C)|FD-Score:8.74|P-value:1.19E-18||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PUN1(YLR414C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Plasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress Gene:RIM8(YGL045W)|FD-Score:-4.13|P-value:1.81E-5||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:ROM2(YLR371W)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPE1(YJL121C)|FD-Score:5.12|P-value:1.49E-7||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL34B(YIL052C)|FD-Score:-3.3|P-value:4.86E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPS21A(YKR057W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication Gene:RPS25A(YGR027C)|FD-Score:3.79|P-value:7.39E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:RRP8(YDR083W)|FD-Score:3.83|P-value:6.38E-5||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:RSC1(YGR056W)|FD-Score:-3.3|P-value:4.83E-4||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:RSC2(YLR357W)|FD-Score:5.34|P-value:4.63E-8||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance Gene:SAP190(YKR028W)|FD-Score:7.66|P-value:9.65E-15||SGD DESC:Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication Gene:SEC72(YLR292C)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SER1(YOR184W)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress Gene:SOD2(YHR008C)|FD-Score:3.76|P-value:8.58E-5||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SOL1(YNR034W)|FD-Score:-3.3|P-value:4.88E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SOL2(YCR073W-A)|FD-Score:-3.1|P-value:9.80E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SPT4(YGR063C)|FD-Score:3.11|P-value:9.27E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SWF1(YDR126W)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:THI74(YDR438W)|FD-Score:-3.89|P-value:5.02E-5||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:THR1(YHR025W)|FD-Score:32|P-value:4.16E-225||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TOM7(YNL070W)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:VAC14(YLR386W)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VAM7(YGL212W)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress Gene:VBA3(YCL069W)|FD-Score:3.72|P-value:9.93E-5||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPS27(YNR006W)|FD-Score:4.89|P-value:5.09E-7||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS3(YDR495C)|FD-Score:4.53|P-value:2.93E-6||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS4(YPR173C)|FD-Score:4.84|P-value:6.53E-7||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:VPS41(YDR080W)|FD-Score:3.14|P-value:8.48E-4||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:VPS60(YDR486C)|FD-Score:4.29|P-value:9.04E-6||SGD DESC:Cytoplasmic and vacuolar membrane protein involved in late endosome to vacuole transport; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p Gene:VPS68(YOL129W)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:YBR200W-A(YBR200W-A_p)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCR050C(YCR050C_p)|FD-Score:5.9|P-value:1.85E-9||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YEL014C(YEL014C_d)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL165C(YGL165C_d)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CUP2/YGL166W Gene:YGL218W(YGL218W_d)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance Gene:YGR161W-C(YGR161W-C_p)|FD-Score:3.74|P-value:9.36E-5||SGD DESC:Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species Gene:YHL008C(YHL008C_p)|FD-Score:-3.19|P-value:7.04E-4||SGD DESC:Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YIM1(YMR152W)|FD-Score:-4.13|P-value:1.83E-5||SGD DESC:Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:YJL120W(YJL120W_d)|FD-Score:3.11|P-value:9.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJR061W(YJR061W_p)|FD-Score:3.73|P-value:9.51E-5||SGD DESC:Putative protein of unknown function; non-essential gene; transcription repressed by Rm101p; YJR061W has a paralog, MNN4, that arose from the whole genome duplication Gene:YJU3(YKL094W)|FD-Score:3.76|P-value:8.53E-5||SGD DESC:Monoglyceride lipase (MGL), functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family Gene:YKL030W(YKL030W_d)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene MAE1 Gene:YKL136W(YKL136W_d)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C Gene:YLR072W(YLR072W_p)|FD-Score:-3.1|P-value:9.56E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene Gene:YLR169W(YLR169W_d)|FD-Score:3.1|P-value:9.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR177W(YLR177W_p)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; not an essential gene; YLR177W has a paralog, PSP1, that arose from the whole genome duplication Gene:YLR415C(YLR415C_p)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Putative protein of unknown function; YLR415C is not an essential gene Gene:YLR422W(YLR422W_p)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Protein of unknown function with similarity to human DOCK proteins (guanine nucleotide exchange factors); interacts with Ino4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, YLR422W is not an essential protein Gene:YNL035C(YNL035C_p)|FD-Score:-3.36|P-value:3.96E-4||SGD DESC:Protein of unknown function; contains WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YOR059C(YOR059C_p)|FD-Score:-4.16|P-value:1.63E-5||SGD DESC:Lipid particle protein of unknown function; contains a putative lipase serine active site; induced by transcription factor Rpn4p; protein abundance increases in response to DNA replication stress Gene:YOR309C(YOR309C_d)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YPR096C(YPR096C)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YTA7(YGR270W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p Gene:ADH6(YMR318C)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:AIM9(YER080W)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:AMS1(YGL156W)|FD-Score:3.87|P-value:5.34E-5||SGD DESC:Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway Gene:ARC18(YLR370C)|FD-Score:-3.13|P-value:8.74E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARG81(YML099C)|FD-Score:-6.15|P-value:3.91E-10||SGD DESC:Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p Gene:ATG19(YOL082W)|FD-Score:-4.43|P-value:4.77E-6||SGD DESC:Receptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathway; delivers cargo proteins aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p) to the phagophore assembly site for packaging into Cvt vesicles Gene:ATP7(YKL016C)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BIK1(YCL029C)|FD-Score:5.79|P-value:3.62E-9||SGD DESC:Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 Gene:BLM10(YFL007W)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Proteasome activator; binds the core proteasome and stimulates proteasome-mediated protein degradation by inducing gate opening; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200 Gene:BUD19(YJL188C_d)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUD28(YLR062C_d)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CCZ1(YBR131W)|FD-Score:3.74|P-value:9.34E-5||SGD DESC:Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway Gene:CRT10(YOL063C)|FD-Score:-3.12|P-value:9.17E-4||SGD DESC:Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat Gene:CSR2(YPR030W)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Nuclear ubiquitin protein ligase binding protein; may regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p; CSR2 has a paralog, ECM21, that arose from the whole genome duplication Gene:DID4(YKL002W)|FD-Score:5.89|P-value:1.90E-9||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:DOC1(YGL240W)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain Gene:DPH5(YLR172C)|FD-Score:4.61|P-value:1.99E-6||SGD DESC:Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm Gene:ELM1(YKL048C)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:ELP2(YGR200C)|FD-Score:4.72|P-value:1.17E-6||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:ELP3(YPL086C)|FD-Score:4.96|P-value:3.61E-7||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin Gene:ELP6(YMR312W)|FD-Score:3.98|P-value:3.38E-5||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:EMI2(YDR516C)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:EXG2(YDR261C)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:FIN1(YDR130C)|FD-Score:-4.45|P-value:4.34E-6||SGD DESC:Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress Gene:GLE2(YER107C)|FD-Score:5|P-value:2.84E-7||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:HOM6(YJR139C)|FD-Score:8.76|P-value:1.01E-18||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HSC82(YMR186W)|FD-Score:3.13|P-value:8.70E-4||SGD DESC:Cytoplasmic chaperone of the Hsp90 family; redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock; contains two acid-rich unstructured regions that promote the solubility of chaperone-substrate complexes; HSC82 has a paralog, HSP82, that arose from the whole genome duplication Gene:IKI1(YHR187W)|FD-Score:4.55|P-value:2.63E-6||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:IKI3(YLR384C)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) Gene:IRE1(YHR079C)|FD-Score:-3.17|P-value:7.66E-4||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:IZH4(YOL101C)|FD-Score:-5.25|P-value:7.49E-8||SGD DESC:Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress Gene:KGD1(YIL125W)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA Gene:LCB5(YLR260W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:LCL2(YLR104W_p)|FD-Score:-3.9|P-value:4.85E-5||SGD DESC:Putative protein of unknown function; mutant is deficient in amounts of cell wall mannosylphosphate and has long chronological lifespan; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:LDB7(YBL006C)|FD-Score:-5.42|P-value:2.99E-8||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LOC1(YFR001W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:LOS1(YKL205W)|FD-Score:3.12|P-value:9.10E-4||SGD DESC:Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:LPX1(YOR084W)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Oleic acid-inducible, peroxisomal matrix localized lipase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; peroxisomal import is dependent on the PTS1 receptor, Pex5p and on self-interaction Gene:LSM1(YJL124C)|FD-Score:4|P-value:3.23E-5||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:LSM7(YNL147W)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:MGA1(YGR249W)|FD-Score:4.04|P-value:2.72E-5||SGD DESC:Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants Gene:MLP1(YKR095W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs Gene:MOG1(YJR074W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:NBA1(YOL070C)|FD-Score:-3.22|P-value:6.37E-4||SGD DESC:Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate Gene:NUT1(YGL151W)|FD-Score:5.54|P-value:1.49E-8||SGD DESC:Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription Gene:OPI9(YLR338W_d)|FD-Score:-6.55|P-value:2.79E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:OSH3(YHR073W)|FD-Score:-4.25|P-value:1.09E-5||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PEP7(YDR323C)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PPT2(YPL148C)|FD-Score:8.74|P-value:1.19E-18||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PUN1(YLR414C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Plasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress Gene:RIM8(YGL045W)|FD-Score:-4.13|P-value:1.81E-5||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:ROM2(YLR371W)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPE1(YJL121C)|FD-Score:5.12|P-value:1.49E-7||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL34B(YIL052C)|FD-Score:-3.3|P-value:4.86E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPS21A(YKR057W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication Gene:RPS25A(YGR027C)|FD-Score:3.79|P-value:7.39E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:RRP8(YDR083W)|FD-Score:3.83|P-value:6.38E-5||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:RSC1(YGR056W)|FD-Score:-3.3|P-value:4.83E-4||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:RSC2(YLR357W)|FD-Score:5.34|P-value:4.63E-8||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance Gene:SAP190(YKR028W)|FD-Score:7.66|P-value:9.65E-15||SGD DESC:Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication Gene:SEC72(YLR292C)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SER1(YOR184W)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress Gene:SOD2(YHR008C)|FD-Score:3.76|P-value:8.58E-5||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SOL1(YNR034W)|FD-Score:-3.3|P-value:4.88E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SOL2(YCR073W-A)|FD-Score:-3.1|P-value:9.80E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SPT4(YGR063C)|FD-Score:3.11|P-value:9.27E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SWF1(YDR126W)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:THI74(YDR438W)|FD-Score:-3.89|P-value:5.02E-5||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:THR1(YHR025W)|FD-Score:32|P-value:4.16E-225||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TOM7(YNL070W)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:VAC14(YLR386W)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VAM7(YGL212W)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress Gene:VBA3(YCL069W)|FD-Score:3.72|P-value:9.93E-5||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPS27(YNR006W)|FD-Score:4.89|P-value:5.09E-7||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS3(YDR495C)|FD-Score:4.53|P-value:2.93E-6||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS4(YPR173C)|FD-Score:4.84|P-value:6.53E-7||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:VPS41(YDR080W)|FD-Score:3.14|P-value:8.48E-4||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:VPS60(YDR486C)|FD-Score:4.29|P-value:9.04E-6||SGD DESC:Cytoplasmic and vacuolar membrane protein involved in late endosome to vacuole transport; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p Gene:VPS68(YOL129W)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:YBR200W-A(YBR200W-A_p)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCR050C(YCR050C_p)|FD-Score:5.9|P-value:1.85E-9||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YEL014C(YEL014C_d)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL165C(YGL165C_d)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CUP2/YGL166W Gene:YGL218W(YGL218W_d)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance Gene:YGR161W-C(YGR161W-C_p)|FD-Score:3.74|P-value:9.36E-5||SGD DESC:Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species Gene:YHL008C(YHL008C_p)|FD-Score:-3.19|P-value:7.04E-4||SGD DESC:Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YIM1(YMR152W)|FD-Score:-4.13|P-value:1.83E-5||SGD DESC:Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:YJL120W(YJL120W_d)|FD-Score:3.11|P-value:9.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJR061W(YJR061W_p)|FD-Score:3.73|P-value:9.51E-5||SGD DESC:Putative protein of unknown function; non-essential gene; transcription repressed by Rm101p; YJR061W has a paralog, MNN4, that arose from the whole genome duplication Gene:YJU3(YKL094W)|FD-Score:3.76|P-value:8.53E-5||SGD DESC:Monoglyceride lipase (MGL), functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family Gene:YKL030W(YKL030W_d)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene MAE1 Gene:YKL136W(YKL136W_d)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C Gene:YLR072W(YLR072W_p)|FD-Score:-3.1|P-value:9.56E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene Gene:YLR169W(YLR169W_d)|FD-Score:3.1|P-value:9.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR177W(YLR177W_p)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; not an essential gene; YLR177W has a paralog, PSP1, that arose from the whole genome duplication Gene:YLR415C(YLR415C_p)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Putative protein of unknown function; YLR415C is not an essential gene Gene:YLR422W(YLR422W_p)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Protein of unknown function with similarity to human DOCK proteins (guanine nucleotide exchange factors); interacts with Ino4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, YLR422W is not an essential protein Gene:YNL035C(YNL035C_p)|FD-Score:-3.36|P-value:3.96E-4||SGD DESC:Protein of unknown function; contains WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YOR059C(YOR059C_p)|FD-Score:-4.16|P-value:1.63E-5||SGD DESC:Lipid particle protein of unknown function; contains a putative lipase serine active site; induced by transcription factor Rpn4p; protein abundance increases in response to DNA replication stress Gene:YOR309C(YOR309C_d)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YPR096C(YPR096C)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YTA7(YGR270W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR016C4.836.76E-70.53TIF6Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
YLR167W4.308.59E-60.11RPS31Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog
YDR473C4.181.43E-50.08PRP3Splicing factor, component of the U4/U6-U5 snRNP complex
YFL002C4.102.03E-50.42SPB4Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients
YLR197W3.681.15E-40.17NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YDR062W3.522.18E-40.14LCB2Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
YML105C3.383.61E-40.00SEC65Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19
YDL030W3.383.66E-40.03PRP9Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex
YGL092W3.354.01E-40.17NUP145Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98
YDL111C3.197.19E-40.07RRP42Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
YGR075C3.129.08E-40.03PRP38Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly
YDR188W3.090.001010.15CCT6Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif
YLR229C2.940.001660.06CDC42Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins
YLR230W_d2.870.002030.07YLR230W_dDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity
YJL195C_d2.800.002560.06YJL195C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR025W32.004.16E-225THR1Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway
YJR139C8.761.01E-18HOM6Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions
YPL148C8.741.19E-18PPT2Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation
YKR028W7.669.65E-15SAP190Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication
YCR050C_p5.901.85E-9YCR050C_pNon-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein
YKL002W5.891.90E-9DID4Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis
YCL029C5.793.62E-9BIK1Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170
YGL151W5.541.49E-8NUT1Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription
YLR357W5.344.63E-8RSC2Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance
YJL121C5.121.49E-7RPE1D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress
YER107C5.002.84E-7GLE2RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1
YPL086C4.963.61E-7ELP3Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin
YNR006W4.895.09E-7VPS27Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)
YPR173C4.846.53E-7VPS4AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism
YGR200C4.721.17E-6ELP2Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin

GO enrichment analysis for SGTC_37
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3343.08E-152SGTC_460329-0187a 2.2 μMChemDiv (Drug-like library)464948170.111111
0.2785.05E-105SGTC_11951502-0147 41.1 μMChemDiv (Drug-like library)8179400.109091
0.2465.04E-82SGTC_1070358-0022 2.7 μMChemDiv (Drug-like library)5211060.139535SWF1 & branched chain AA biosynthesis
0.2177.61E-64SGTC_900870-0863 4.6 μMChemDiv (Drug-like library)17381640.218182
0.1885.19E-48SGTC_1954234-0266 16.7 μMChemDiv (Drug-like library)23033540.0714286
0.1879.71E-47SGTC_3710195-0006 7.4 μMChemDiv (Drug-like library)39285930.130435
0.1782.70E-43SGTC_1771327-0068 8.4 μMChemDiv (Drug-like library)153899000.215686
0.1641.03E-36SGTC_1696st025591 34.8 μMTimTec (Natural product derivative library)27871470.155172SWF1 & branched chain AA biosynthesis
0.1582.80E-34SGTC_2689tacrolimus 100.0 μMNIH Clinical Collection235817960.0442478
0.1571.23E-33SGTC_2233sulbentine 40.4 μMMiscellaneous676860.117647
0.1511.37E-31SGTC_163TUB2.036 7.5 μMChemDiv (Drug-like library)159922070.0615385
0.1504.02E-31SGTC_1593970-0959 50.0 μMChemDiv (Drug-like library)66156530.0985916
0.1345.08E-25SGTC_1844rapamycin 4.0 nMMiscellaneous52846160.0238095
0.1345.51E-25SGTC_451chloral hydrate 100.0 μMMiscellaneous2707NA
0.1314.37E-24SGTC_24305571206 200.0 μMMiscellaneous17317710.131148

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1608st00147340.5 μM0.4210537292267TimTec (Natural product derivative library)242.700244.36601
SGTC_26044'-hydroxychalcone11.32 μM0.3846155282362Microsource (Natural product library)224.254583.4612
SGTC_1691st01993384.6 μM0.3809526176082TimTec (Natural product derivative library)236.308344.67401
SGTC_19284'-methoxychalcone40.3 μM0.357143641818TimTec (Natural product derivative library)238.281163.68502
SGTC_24984'-Methoxychalcone54.63 μM0.357143641818Microsource (Natural product library)238.281163.68502RPP1 & pyrimidine depletion
SGTC_7991310-0557151 μM0.3555565354492ChemDiv (Drug-like library)252.264683.4703superoxide
SGTC_1813st0512468.6 μM0.3488375291959TimTec (Natural product derivative library)253.252743.59603TSC3-RPN4
SGTC_2701st07776714.72 μM0.3478265721188TimTec (Natural product derivative library)281.329044.46903
SGTC_1726st03555614.58 μM0.3181825919439TimTec (Natural product derivative library)260.6907034.57102
SGTC_6001495-041841.6 μM0.3043485377768ChemDiv (Drug-like library)267.345524.36603RPP1 & pyrimidine depletion