0180-0345

3,5-dichloro-2-hydroxy-N-(3,4,5-trichlorophenyl)benzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_370
Screen concentration 38.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 3989527
SMILES C1=C(C=C(C(=C1Cl)Cl)Cl)NC(=O)C2=CC(=CC(=C2O)Cl)Cl
Standardized SMILES Oc1c(Cl)cc(Cl)cc1C(=O)Nc2cc(Cl)c(Cl)c(Cl)c2
Molecular weight 385.4572
ALogP 5.69
H-bond donor count 2
H-bond acceptor count 2
Response signature DNA intercalators

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.35
% growth inhibition (Hom. pool) 2.62


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3989527
Download HIP data (tab-delimited text)  (excel)
Gene:AOS1(YPR180W)|FD-Score:-3.39|P-value:3.46E-4|Clearance:0||SGD DESC:Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability Gene:ARC19(YKL013C)|FD-Score:3.47|P-value:2.60E-4|Clearance:0.03||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:CDC16(YKL022C)|FD-Score:-3.71|P-value:1.02E-4|Clearance:0||SGD DESC:Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation Gene:ERG12(YMR208W)|FD-Score:5.58|P-value:1.22E-8|Clearance:0.84||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:GLC7(YER133W)|FD-Score:4.74|P-value:1.06E-6|Clearance:0.32||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:IPP1(YBR011C)|FD-Score:3.3|P-value:4.91E-4|Clearance:0.11||SGD DESC:Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase Gene:MCD4(YKL165C)|FD-Score:4.2|P-value:1.35E-5|Clearance:0.14||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:NOC3(YLR002C)|FD-Score:-3.98|P-value:3.45E-5|Clearance:0||SGD DESC:Component of a nuclear complex with Noc2p and pre-replicative complexes; the Noc2p-Noc3p complex binds to 66S ribosomal precursors to mediate their maturation and intranuclear transport; binds to chromatin at active replication origins, and is required for pre-RC formation and maintenance during DNA replication licensing Gene:PFS2(YNL317W)|FD-Score:3.9|P-value:4.78E-5|Clearance:0.43||SGD DESC:Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex Gene:PKC1(YBL105C)|FD-Score:3.34|P-value:4.20E-4|Clearance:0.04||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PMI40(YER003C)|FD-Score:3.44|P-value:2.86E-4|Clearance:0.11||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:RPC11(YDR045C)|FD-Score:4.06|P-value:2.47E-5|Clearance:0.16||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:RRN7(YJL025W)|FD-Score:4.29|P-value:9.05E-6|Clearance:0.03||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SEC26(YDR238C)|FD-Score:-5.27|P-value:6.80E-8|Clearance:0||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SKP1(YDR328C)|FD-Score:4.42|P-value:4.87E-6|Clearance:0.14||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:SPC97(YHR172W)|FD-Score:7.65|P-value:1.03E-14|Clearance:2.07||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TFB4(YPR056W)|FD-Score:3.19|P-value:7.12E-4|Clearance:0.12||SGD DESC:Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p Gene:TIF35(YDR429C)|FD-Score:-3.19|P-value:7.21E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:UFE1(YOR075W)|FD-Score:4.26|P-value:1.04E-5|Clearance:0.06||SGD DESC:t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER Gene:UTP4(YDR324C)|FD-Score:-4.06|P-value:2.44E-5|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YPL044C(YPL044C_d)|FD-Score:-3.48|P-value:2.49E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W Gene:YPR142C(YPR142C_d)|FD-Score:-6.83|P-value:4.12E-12|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing Gene:AOS1(YPR180W)|FD-Score:-3.39|P-value:3.46E-4|Clearance:0||SGD DESC:Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability Gene:ARC19(YKL013C)|FD-Score:3.47|P-value:2.60E-4|Clearance:0.03||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:CDC16(YKL022C)|FD-Score:-3.71|P-value:1.02E-4|Clearance:0||SGD DESC:Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation Gene:ERG12(YMR208W)|FD-Score:5.58|P-value:1.22E-8|Clearance:0.84||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:GLC7(YER133W)|FD-Score:4.74|P-value:1.06E-6|Clearance:0.32||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:IPP1(YBR011C)|FD-Score:3.3|P-value:4.91E-4|Clearance:0.11||SGD DESC:Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase Gene:MCD4(YKL165C)|FD-Score:4.2|P-value:1.35E-5|Clearance:0.14||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:NOC3(YLR002C)|FD-Score:-3.98|P-value:3.45E-5|Clearance:0||SGD DESC:Component of a nuclear complex with Noc2p and pre-replicative complexes; the Noc2p-Noc3p complex binds to 66S ribosomal precursors to mediate their maturation and intranuclear transport; binds to chromatin at active replication origins, and is required for pre-RC formation and maintenance during DNA replication licensing Gene:PFS2(YNL317W)|FD-Score:3.9|P-value:4.78E-5|Clearance:0.43||SGD DESC:Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex Gene:PKC1(YBL105C)|FD-Score:3.34|P-value:4.20E-4|Clearance:0.04||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PMI40(YER003C)|FD-Score:3.44|P-value:2.86E-4|Clearance:0.11||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:RPC11(YDR045C)|FD-Score:4.06|P-value:2.47E-5|Clearance:0.16||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:RRN7(YJL025W)|FD-Score:4.29|P-value:9.05E-6|Clearance:0.03||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SEC26(YDR238C)|FD-Score:-5.27|P-value:6.80E-8|Clearance:0||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SKP1(YDR328C)|FD-Score:4.42|P-value:4.87E-6|Clearance:0.14||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:SPC97(YHR172W)|FD-Score:7.65|P-value:1.03E-14|Clearance:2.07||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TFB4(YPR056W)|FD-Score:3.19|P-value:7.12E-4|Clearance:0.12||SGD DESC:Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p Gene:TIF35(YDR429C)|FD-Score:-3.19|P-value:7.21E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:UFE1(YOR075W)|FD-Score:4.26|P-value:1.04E-5|Clearance:0.06||SGD DESC:t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER Gene:UTP4(YDR324C)|FD-Score:-4.06|P-value:2.44E-5|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YPL044C(YPL044C_d)|FD-Score:-3.48|P-value:2.49E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W Gene:YPR142C(YPR142C_d)|FD-Score:-6.83|P-value:4.12E-12|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3989527
Download HOP data (tab-delimited text)  (excel)
Gene:ARC1(YGL105W)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress Gene:ARC18(YLR370C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ASI1(YMR119W)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:BUD25(YER014C-A)|FD-Score:3.84|P-value:6.18E-5||SGD DESC:Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern Gene:BUD32(YGR262C)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Protein kinase, component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:CHD1(YER164W)|FD-Score:4.76|P-value:9.88E-7||SGD DESC:Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes Gene:CUL3(YGR003W)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3 Gene:EFT1(YOR133W)|FD-Score:5.11|P-value:1.60E-7||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication Gene:EMP24(YGL200C)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:ERP3(YDL018C)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:GCV3(YAL044C)|FD-Score:-3.38|P-value:3.62E-4||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GPB2(YAL056W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication Gene:GTO3(YMR251W)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:HBT1(YDL223C)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HHT2(YNL031C)|FD-Score:-3.81|P-value:7.01E-5||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation Gene:HOM2(YDR158W)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:ISF1(YMR081C)|FD-Score:-4.65|P-value:1.67E-6||SGD DESC:Serine-rich, hydrophilic protein with similarity to Mbr1p; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; expression is under glucose control; cotranscribed with NAM7 in a cyp1 mutant Gene:IST3(YIR005W)|FD-Score:-3.22|P-value:6.48E-4||SGD DESC:Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing Gene:JHD2(YJR119C)|FD-Score:-3.19|P-value:7.18E-4||SGD DESC:JmjC domain family histone demethylase specific for H3-K4 (histone H3 Lys4); removes methyl groups specifically added by Set1p methyltransferase; protein levels regulated by Not4p (E3 ubiquitin ligase) polyubiquitin-mediated degradation Gene:LDH1(YBR204C)|FD-Score:-5.85|P-value:2.50E-9||SGD DESC:Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif) Gene:ORT1(YOR130C)|FD-Score:6.05|P-value:7.24E-10||SGD DESC:Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome Gene:PEP3(YLR148W)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PIB1(YDR313C)|FD-Score:-3.32|P-value:4.49E-4||SGD DESC:RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain Gene:PMT2(YAL023C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:PNT1(YOR266W)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Mitochondrial integral inner membrane protein involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p; deletion mutant sensitive to the anti-Pneumocystis carinii drug pentamidine Gene:RAD5(YLR032W)|FD-Score:-4.9|P-value:4.75E-7||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RIC1(YLR039C)|FD-Score:7.99|P-value:6.66E-16||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RIP1(YEL024W)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly Gene:ROM1(YGR070W)|FD-Score:3.87|P-value:5.44E-5||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:RPL8A(YHL033C)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RPS14A(YCR031C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RTC5(YOR118W)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; null mutation suppresses cdc13-1 temperature sensitivity Gene:SET5(YHR207C)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SMI1(YGR229C)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SMK1(YPR054W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p Gene:STV1(YMR054W)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:SUR4(YLR372W)|FD-Score:7.05|P-value:8.78E-13||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TES1(YJR019C)|FD-Score:-3.2|P-value:6.88E-4||SGD DESC:Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids Gene:TSR2(YLR435W)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:UBP12(YJL197W)|FD-Score:-4.64|P-value:1.76E-6||SGD DESC:Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm Gene:VID22(YLR373C)|FD-Score:-4.22|P-value:1.20E-5||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:YAL037C-A(YAL037C-A_p)|FD-Score:-4.26|P-value:1.02E-5||SGD DESC:Putative protein of unknown function Gene:YBL028C(YBL028C)|FD-Score:-3.48|P-value:2.46E-4||SGD DESC:Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:YBR090C(YBR090C_p)|FD-Score:-3.09|P-value:9.96E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YDR109C(YDR109C_p)|FD-Score:-3.1|P-value:9.57E-4||SGD DESC:Putative kinase Gene:YEL010W(YEL010W_d)|FD-Score:4.56|P-value:2.52E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL088W(YGL088W_d)|FD-Score:4.33|P-value:7.59E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YGR153W(YGR153W_p)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Putative protein of unknown function Gene:YJL119C(YJL119C_d)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL030W(YKL030W_d)|FD-Score:-3.88|P-value:5.28E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene MAE1 Gene:YKL100C(YKL100C_p)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Putative protein of unknown function with similarity to a human minor histocompatibility antigen and signal peptide peptidases; YKL100C is not an essential gene Gene:YKR078W(YKR078W)|FD-Score:-4.46|P-value:4.02E-6||SGD DESC:Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:YLL047W(YLL047W_d)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1 Gene:YLR279W(YLR279W_d)|FD-Score:-3.37|P-value:3.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL057W(YNL057W_d)|FD-Score:-5.31|P-value:5.38E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL285W(YNL285W_d)|FD-Score:4.94|P-value:3.87E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR199W(YOR199W_d)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR293C-A(YOR293C-A_p)|FD-Score:3.99|P-value:3.29E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR366W(YOR366W_d)|FD-Score:3.73|P-value:9.59E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C Gene:ARC1(YGL105W)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress Gene:ARC18(YLR370C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ASI1(YMR119W)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:BUD25(YER014C-A)|FD-Score:3.84|P-value:6.18E-5||SGD DESC:Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern Gene:BUD32(YGR262C)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Protein kinase, component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:CHD1(YER164W)|FD-Score:4.76|P-value:9.88E-7||SGD DESC:Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes Gene:CUL3(YGR003W)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3 Gene:EFT1(YOR133W)|FD-Score:5.11|P-value:1.60E-7||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication Gene:EMP24(YGL200C)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:ERP3(YDL018C)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:GCV3(YAL044C)|FD-Score:-3.38|P-value:3.62E-4||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GPB2(YAL056W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication Gene:GTO3(YMR251W)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:HBT1(YDL223C)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HHT2(YNL031C)|FD-Score:-3.81|P-value:7.01E-5||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation Gene:HOM2(YDR158W)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:ISF1(YMR081C)|FD-Score:-4.65|P-value:1.67E-6||SGD DESC:Serine-rich, hydrophilic protein with similarity to Mbr1p; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; expression is under glucose control; cotranscribed with NAM7 in a cyp1 mutant Gene:IST3(YIR005W)|FD-Score:-3.22|P-value:6.48E-4||SGD DESC:Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing Gene:JHD2(YJR119C)|FD-Score:-3.19|P-value:7.18E-4||SGD DESC:JmjC domain family histone demethylase specific for H3-K4 (histone H3 Lys4); removes methyl groups specifically added by Set1p methyltransferase; protein levels regulated by Not4p (E3 ubiquitin ligase) polyubiquitin-mediated degradation Gene:LDH1(YBR204C)|FD-Score:-5.85|P-value:2.50E-9||SGD DESC:Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif) Gene:ORT1(YOR130C)|FD-Score:6.05|P-value:7.24E-10||SGD DESC:Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome Gene:PEP3(YLR148W)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PIB1(YDR313C)|FD-Score:-3.32|P-value:4.49E-4||SGD DESC:RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain Gene:PMT2(YAL023C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:PNT1(YOR266W)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Mitochondrial integral inner membrane protein involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p; deletion mutant sensitive to the anti-Pneumocystis carinii drug pentamidine Gene:RAD5(YLR032W)|FD-Score:-4.9|P-value:4.75E-7||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RIC1(YLR039C)|FD-Score:7.99|P-value:6.66E-16||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RIP1(YEL024W)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly Gene:ROM1(YGR070W)|FD-Score:3.87|P-value:5.44E-5||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:RPL8A(YHL033C)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RPS14A(YCR031C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RTC5(YOR118W)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; null mutation suppresses cdc13-1 temperature sensitivity Gene:SET5(YHR207C)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SMI1(YGR229C)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SMK1(YPR054W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p Gene:STV1(YMR054W)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:SUR4(YLR372W)|FD-Score:7.05|P-value:8.78E-13||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TES1(YJR019C)|FD-Score:-3.2|P-value:6.88E-4||SGD DESC:Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids Gene:TSR2(YLR435W)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:UBP12(YJL197W)|FD-Score:-4.64|P-value:1.76E-6||SGD DESC:Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm Gene:VID22(YLR373C)|FD-Score:-4.22|P-value:1.20E-5||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:YAL037C-A(YAL037C-A_p)|FD-Score:-4.26|P-value:1.02E-5||SGD DESC:Putative protein of unknown function Gene:YBL028C(YBL028C)|FD-Score:-3.48|P-value:2.46E-4||SGD DESC:Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:YBR090C(YBR090C_p)|FD-Score:-3.09|P-value:9.96E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YDR109C(YDR109C_p)|FD-Score:-3.1|P-value:9.57E-4||SGD DESC:Putative kinase Gene:YEL010W(YEL010W_d)|FD-Score:4.56|P-value:2.52E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL088W(YGL088W_d)|FD-Score:4.33|P-value:7.59E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YGR153W(YGR153W_p)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Putative protein of unknown function Gene:YJL119C(YJL119C_d)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL030W(YKL030W_d)|FD-Score:-3.88|P-value:5.28E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene MAE1 Gene:YKL100C(YKL100C_p)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Putative protein of unknown function with similarity to a human minor histocompatibility antigen and signal peptide peptidases; YKL100C is not an essential gene Gene:YKR078W(YKR078W)|FD-Score:-4.46|P-value:4.02E-6||SGD DESC:Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:YLL047W(YLL047W_d)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1 Gene:YLR279W(YLR279W_d)|FD-Score:-3.37|P-value:3.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL057W(YNL057W_d)|FD-Score:-5.31|P-value:5.38E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL285W(YNL285W_d)|FD-Score:4.94|P-value:3.87E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR199W(YOR199W_d)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR293C-A(YOR293C-A_p)|FD-Score:3.99|P-value:3.29E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR366W(YOR366W_d)|FD-Score:3.73|P-value:9.59E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR172W7.651.03E-142.07SPC97Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YMR208W5.581.22E-80.84ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YER133W4.741.06E-60.32GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YDR328C4.424.87E-60.14SKP1Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
YJL025W4.299.05E-60.03RRN7Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
YOR075W4.261.04E-50.06UFE1t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER
YKL165C4.201.35E-50.14MCD4Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes
YDR045C4.062.47E-50.16RPC11RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS
YNL317W3.904.78E-50.43PFS2Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex
YKL013C3.472.60E-40.03ARC19Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YER003C3.442.86E-40.10PMI40Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
YBL105C3.344.20E-40.04PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YBR011C3.304.91E-40.11IPP1Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase
YPR056W3.197.12E-40.12TFB4Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p
YFR027W3.070.001070.01ECO1Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR039C7.996.66E-16RIC1Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes
YLR372W7.058.78E-13SUR4Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
YOR130C6.057.24E-10ORT1Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome
YOR133W5.111.60E-7EFT1Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication
YNL285W_d4.943.87E-7YNL285W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YER164W4.769.88E-7CHD1Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes
YEL010W_d4.562.52E-6YEL010W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YGL088W_d4.337.59E-6YGL088W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing
YHL033C4.052.57E-5RPL8ARibosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication
YGR229C4.022.88E-5SMI1Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity
YOR293C-A_p3.993.29E-5YOR293C-A_pPutative protein of unknown function; identified by expression profiling and mass spectrometry
YGR070W3.875.44E-5ROM1GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP
YER014C-A3.846.18E-5BUD25Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern
YOR366W_d3.739.59E-5YOR366W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C
YOR199W_d3.671.21E-4YOR199W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_370
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1552.77E-32SGTC_30409091894 49.5 μMChembridge (Drug-like library)252369660.0882353DNA intercalators
0.1518.87E-31SGTC_4711-acyl-paf 4.0 μMICCB bioactive library167593670.04DNA intercalators
0.1295.93E-23SGTC_8670518-0118 155.0 μMChemDiv (Drug-like library)68481580.160714DNA intercalators
0.1203.85E-20SGTC_1533dihydroergotamine mesylate 29.4 μMTimTec (Pure natural product library)711710.0642202DNA intercalators
0.1198.94E-20SGTC_14153966-0071 78.1 μMChemDiv (Drug-like library)28931850.0961538DNA intercalators
0.1152.14E-18SGTC_264promethazine 12.5 mMMiscellaneous60140.0344828DNA intercalators
0.1072.42E-16SGTC_1250133-0007 11.8 μMChemDiv (Drug-like library)265410.0434783DNA intercalators
0.1034.37E-15SGTC_512prima-1 270.0 μMICCB bioactive library3229680.0576923DNA intercalators
0.0966.04E-13SGTC_1460k062-0059 9.2 μMChemDiv (Drug-like library)13730850.22807Golgi
0.0906.52E-12SGTC_511blebbistatin 171.0 μMICCB bioactive library34769860.0724638DNA intercalators
0.0891.38E-11SGTC_1444161-2749 112.4 μMChemDiv (Drug-like library)7975010.169492DNA intercalators
0.0872.81E-11SGTC_850868-0259 160.1 μMChemDiv (Drug-like library)831730.14545560S ribosome export
0.0832.97E-10SGTC_1926methyl fluorone black 53.6 μMTimTec (Natural product derivative library)727210.103448DNA intercalators
0.0817.60E-10SGTC_470aa-861 153.0 μMICCB bioactive library19670.0517241sphingolipid biosynthesis & PDR1
0.0792.01E-9SGTC_2740felodipine 17.1 μMMiscellaneous33330.138462

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1310141-027733.85 μM0.4680851547771ChemDiv (Drug-like library)419.49575.0222
SGTC_700141-028943.28 μM0.444444352408ChemDiv (Drug-like library)405.469124.53322mitochondrial stress
SGTC_1190121-004324.82 μM0.4166671092378ChemDiv (Drug-like library)405.469124.53322mitochondrial stress
SGTC_2931901467455.67 μM0.3921572996810Chembridge (Drug-like library)326.174624.03323
SGTC_2230bromochlorosalicylanilide954.5 nM0.39130477254Miscellaneous326.573063.78522
SGTC_2872904403725.97 μM0.3777786465195Chembridge (Drug-like library)284.1131234.14912
SGTC_2942905277762.25 μM0.3541676469503Chembridge (Drug-like library)284.1131234.14912
SGTC_3650121-005213.6 μM0.3529413101739ChemDiv (Drug-like library)361.564684.2624mitochondrial response to ROS
SGTC_7600568-072582.9 μM0.343916171ChemDiv (Drug-like library)380.010345.16613
SGTC_1270180-033359.13 μM0.333333771601ChemDiv (Drug-like library)324.158743.71113mitochondrial response to ROS