0155-0182

[4-(dimethylamino)phenyl]methylidene-(2-hydroxyphenyl)azanium

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_372
Screen concentration 15.9 μM
Source ChemDiv (Drug-like library)
PubChem CID 771486
SMILES CN(C)C1=CC=C(C=C1)C=[NH+]C2=CC=CC=C2O
Standardized SMILES CN(C)c1ccc(C=[NH+]c2ccccc2O)cc1
Molecular weight 241.3083
ALogP 3.24
H-bond donor count 2
H-bond acceptor count 2
Response signature mitochondrial response to ROS

Pool Growth Kinetics
% growth inhibition (Het. pool) 64.3
% growth inhibition (Hom. pool) 12.83


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 771486
Download HIP data (tab-delimited text)  (excel)
Gene:ACP1(YKL192C)|FD-Score:3.33|P-value:4.41E-4|Clearance:0.08||SGD DESC:Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p Gene:ACT1(YFL039C)|FD-Score:-4.86|P-value:5.89E-7|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:AUR1(YKL004W)|FD-Score:-4.45|P-value:4.24E-6|Clearance:0||SGD DESC:Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance Gene:BUR6(YER159C)|FD-Score:-4.05|P-value:2.57E-5|Clearance:0||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha Gene:CAB2(YIL083C)|FD-Score:-7.04|P-value:9.54E-13|Clearance:0||SGD DESC:Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity) Gene:CCT4(YDL143W)|FD-Score:-6.73|P-value:8.31E-12|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC20(YGL116W)|FD-Score:-5.12|P-value:1.51E-7|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC39(YCR093W)|FD-Score:-9.81|P-value:5.21E-23|Clearance:0||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CDC42(YLR229C)|FD-Score:-4.59|P-value:2.16E-6|Clearance:0||SGD DESC:Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins Gene:CDC48(YDL126C)|FD-Score:-5.39|P-value:3.55E-8|Clearance:0||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:DIP2(YLR129W)|FD-Score:-5.36|P-value:4.23E-8|Clearance:0||SGD DESC:Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex Gene:DNA2(YHR164C)|FD-Score:-6.65|P-value:1.43E-11|Clearance:0||SGD DESC:Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress Gene:DPB11(YJL090C)|FD-Score:-5.35|P-value:4.33E-8|Clearance:0||SGD DESC:DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress Gene:DUT1(YBR252W)|FD-Score:-3.35|P-value:4.04E-4|Clearance:0||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:EFB1(YAL003W)|FD-Score:-4.47|P-value:4.00E-6|Clearance:0||SGD DESC:Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site Gene:ERG11(YHR007C)|FD-Score:-6.25|P-value:2.02E-10|Clearance:0||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ESP1(YGR098C)|FD-Score:-6.95|P-value:1.89E-12|Clearance:0||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:FAS1(YKL182W)|FD-Score:-3.42|P-value:3.14E-4|Clearance:0||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:FAS2(YPL231W)|FD-Score:-7.87|P-value:1.79E-15|Clearance:0||SGD DESC:Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities Gene:GCD2(YGR083C)|FD-Score:-3.76|P-value:8.37E-5|Clearance:0||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GLC7(YER133W)|FD-Score:-11.4|P-value:1.78E-30|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GLN4(YOR168W)|FD-Score:-5.15|P-value:1.32E-7|Clearance:0||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:GPI11(YDR302W)|FD-Score:-4.08|P-value:2.22E-5|Clearance:0||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:GPM1(YKL152C)|FD-Score:3.24|P-value:5.94E-4|Clearance:0.05||SGD DESC:Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis Gene:HRR25(YPL204W)|FD-Score:-4.99|P-value:3.08E-7|Clearance:0||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:HSH49(YOR319W)|FD-Score:-5.43|P-value:2.78E-8|Clearance:0||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:IFH1(YLR223C)|FD-Score:3.65|P-value:1.32E-4|Clearance:0.06||SGD DESC:Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing Gene:ILS1(YBL076C)|FD-Score:-3.9|P-value:4.87E-5|Clearance:0||SGD DESC:Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A Gene:LCB2(YDR062W)|FD-Score:7.72|P-value:5.84E-15|Clearance:1.27||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:LCP5(YER127W)|FD-Score:-5.54|P-value:1.55E-8|Clearance:0||SGD DESC:Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus Gene:LSM5(YER146W)|FD-Score:-3.51|P-value:2.24E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MCM10(YIL150C)|FD-Score:-4.1|P-value:2.09E-5|Clearance:0||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:MED7(YOL135C)|FD-Score:-4.02|P-value:2.90E-5|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MEX67(YPL169C)|FD-Score:-4.97|P-value:3.38E-7|Clearance:0||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:MIM1(YOL026C)|FD-Score:-8.29|P-value:5.59E-17|Clearance:0||SGD DESC:Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex Gene:MMS21(YEL019C)|FD-Score:-7.34|P-value:1.09E-13|Clearance:0||SGD DESC:SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination Gene:MRS6(YOR370C)|FD-Score:-7.89|P-value:1.56E-15|Clearance:0||SGD DESC:Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress Gene:NDC1(YML031W)|FD-Score:-3.5|P-value:2.31E-4|Clearance:0||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:NIP1(YMR309C)|FD-Score:-6.57|P-value:2.60E-11|Clearance:0||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NOP58(YOR310C)|FD-Score:-3.96|P-value:3.69E-5|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NUP1(YOR098C)|FD-Score:6.45|P-value:5.54E-11|Clearance:0.85||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153 Gene:ORC1(YML065W)|FD-Score:-3.42|P-value:3.19E-4|Clearance:0||SGD DESC:Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity Gene:ORC4(YPR162C)|FD-Score:3.58|P-value:1.69E-4|Clearance:0.01||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:ORC6(YHR118C)|FD-Score:-5.27|P-value:6.92E-8|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p Gene:PGA2(YNL149C)|FD-Score:-6.82|P-value:4.68E-12|Clearance:0||SGD DESC:Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect Gene:PRE10(YOR362C)|FD-Score:-3.95|P-value:3.95E-5|Clearance:0||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE2(YPR103W)|FD-Score:-3.14|P-value:8.43E-4|Clearance:0||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:PRE5(YMR314W)|FD-Score:-4.75|P-value:9.93E-7|Clearance:0||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE6(YOL038W)|FD-Score:-9.75|P-value:8.99E-23|Clearance:0||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRP24(YMR268C)|FD-Score:-9.48|P-value:1.30E-21|Clearance:0||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:PRP39(YML046W)|FD-Score:-5.53|P-value:1.60E-8|Clearance:0||SGD DESC:U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats Gene:PRP45(YAL032C)|FD-Score:-3.4|P-value:3.39E-4|Clearance:0||SGD DESC:Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene Gene:PRP9(YDL030W)|FD-Score:-4.28|P-value:9.34E-6|Clearance:0||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex Gene:PUP1(YOR157C)|FD-Score:-6.68|P-value:1.16E-11|Clearance:0||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:PUP2(YGR253C)|FD-Score:-3.17|P-value:7.56E-4|Clearance:0||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:PUP3(YER094C)|FD-Score:-4.25|P-value:1.09E-5|Clearance:0||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:RAD3(YER171W)|FD-Score:-5.06|P-value:2.15E-7|Clearance:0||SGD DESC:5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress Gene:RET3(YPL010W)|FD-Score:3.2|P-value:6.98E-4|Clearance:0.05||SGD DESC:Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RNA14(YMR061W)|FD-Score:-4.66|P-value:1.59E-6|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RNA15(YGL044C)|FD-Score:-6.78|P-value:6.18E-12|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPB5(YBR154C)|FD-Score:-5.35|P-value:4.29E-8|Clearance:0||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPB8(YOR224C)|FD-Score:-3.9|P-value:4.83E-5|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPN11(YFR004W)|FD-Score:-3.11|P-value:9.50E-4|Clearance:0||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RPO26(YPR187W)|FD-Score:-3.45|P-value:2.80E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPP1(YHR062C)|FD-Score:-5.63|P-value:9.14E-9|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS13(YDR064W)|FD-Score:-3.1|P-value:9.58E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPS15(YOL040C)|FD-Score:-5.25|P-value:7.72E-8|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPS31(YLR167W)|FD-Score:-11.3|P-value:5.74E-30|Clearance:0||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:RPS5(YJR123W)|FD-Score:-7.72|P-value:5.81E-15|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:RRP43(YCR035C)|FD-Score:-3.42|P-value:3.18E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:RTT105(YER104W)|FD-Score:-6.89|P-value:2.75E-12|Clearance:0||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SAM50(YNL026W)|FD-Score:-3.26|P-value:5.49E-4|Clearance:0||SGD DESC:Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 Gene:SAS10(YDL153C)|FD-Score:-5.23|P-value:8.59E-8|Clearance:0||SGD DESC:Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance Gene:SDA1(YGR245C)|FD-Score:-10.6|P-value:1.26E-26|Clearance:0||SGD DESC:Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis Gene:SEC21(YNL287W)|FD-Score:3.15|P-value:8.25E-4|Clearance:0.29||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SEC26(YDR238C)|FD-Score:5.6|P-value:1.09E-8|Clearance:0.35||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SEC31(YDL195W)|FD-Score:-4.83|P-value:6.86E-7|Clearance:0||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEC62(YPL094C)|FD-Score:3.43|P-value:3.02E-4|Clearance:0.1||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SES1(YDR023W)|FD-Score:-4.39|P-value:5.69E-6|Clearance:0||SGD DESC:Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p Gene:SKP1(YDR328C)|FD-Score:-3.42|P-value:3.17E-4|Clearance:0||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:SMC5(YOL034W)|FD-Score:-6.14|P-value:4.23E-10|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair Gene:SMD1(YGR074W)|FD-Score:-4.02|P-value:2.94E-5|Clearance:0||SGD DESC:Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress Gene:SNM1(YDR478W)|FD-Score:-4.19|P-value:1.41E-5|Clearance:0||SGD DESC:Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP Gene:SNU56(YDR240C)|FD-Score:3.7|P-value:1.10E-4|Clearance:0.05||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex Gene:SOF1(YLL011W)|FD-Score:-11.8|P-value:2.32E-32|Clearance:0||SGD DESC:Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p Gene:SPC42(YKL042W)|FD-Score:-7.15|P-value:4.42E-13|Clearance:0||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:SPT14(YPL175W)|FD-Score:3.57|P-value:1.76E-4|Clearance:0.14||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:SRB4(YER022W)|FD-Score:-4.49|P-value:3.54E-6|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination Gene:STU2(YLR045C)|FD-Score:-3.24|P-value:5.96E-4|Clearance:0||SGD DESC:Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p Gene:SUI1(YNL244C)|FD-Score:-6.53|P-value:3.26E-11|Clearance:0||SGD DESC:Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase Gene:THS1(YIL078W)|FD-Score:-9.47|P-value:1.42E-21|Clearance:0||SGD DESC:Threonyl-tRNA synthetase, essential cytoplasmic protein Gene:TIM17(YJL143W)|FD-Score:-3.35|P-value:4.04E-4|Clearance:0||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TOM22(YNL131W)|FD-Score:-3.68|P-value:1.15E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:TRS130(YMR218C)|FD-Score:-5|P-value:2.84E-7|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy Gene:TRS31(YDR472W)|FD-Score:-6.66|P-value:1.38E-11|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TSR1(YDL060W)|FD-Score:-4.72|P-value:1.15E-6|Clearance:0||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:TTI1(YKL033W)|FD-Score:-5.26|P-value:7.32E-8|Clearance:0||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies Gene:UTP14(YML093W)|FD-Score:-7.47|P-value:3.87E-14|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:UTP15(YMR093W)|FD-Score:-6.79|P-value:5.45E-12|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTP5(YDR398W)|FD-Score:-5.03|P-value:2.41E-7|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:WRS1(YOL097C)|FD-Score:-6.01|P-value:8.99E-10|Clearance:0||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YGL069C(YGL069C_d)|FD-Score:5.24|P-value:7.86E-8|Clearance:0.36||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance Gene:YGL074C(YGL074C_d)|FD-Score:-13.6|P-value:1.25E-42|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor Gene:YGR073C(YGR073C_d)|FD-Score:-4.58|P-value:2.34E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein Gene:YKL153W(YKL153W_d)|FD-Score:-4.66|P-value:1.57E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant Gene:YLR140W(YLR140W_d)|FD-Score:4.88|P-value:5.32E-7|Clearance:0.95||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I Gene:YLR317W(YLR317W_d)|FD-Score:3.93|P-value:4.24E-5|Clearance:0.23||SGD DESC:Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C Gene:YNL114C(YNL114C_d)|FD-Score:-3.21|P-value:6.62E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YPL251W(YPL251W_d)|FD-Score:-3.99|P-value:3.35E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C Gene:ACP1(YKL192C)|FD-Score:3.33|P-value:4.41E-4|Clearance:0.08||SGD DESC:Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p Gene:ACT1(YFL039C)|FD-Score:-4.86|P-value:5.89E-7|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:AUR1(YKL004W)|FD-Score:-4.45|P-value:4.24E-6|Clearance:0||SGD DESC:Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance Gene:BUR6(YER159C)|FD-Score:-4.05|P-value:2.57E-5|Clearance:0||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha Gene:CAB2(YIL083C)|FD-Score:-7.04|P-value:9.54E-13|Clearance:0||SGD DESC:Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity) Gene:CCT4(YDL143W)|FD-Score:-6.73|P-value:8.31E-12|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC20(YGL116W)|FD-Score:-5.12|P-value:1.51E-7|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC39(YCR093W)|FD-Score:-9.81|P-value:5.21E-23|Clearance:0||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CDC42(YLR229C)|FD-Score:-4.59|P-value:2.16E-6|Clearance:0||SGD DESC:Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins Gene:CDC48(YDL126C)|FD-Score:-5.39|P-value:3.55E-8|Clearance:0||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:DIP2(YLR129W)|FD-Score:-5.36|P-value:4.23E-8|Clearance:0||SGD DESC:Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex Gene:DNA2(YHR164C)|FD-Score:-6.65|P-value:1.43E-11|Clearance:0||SGD DESC:Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress Gene:DPB11(YJL090C)|FD-Score:-5.35|P-value:4.33E-8|Clearance:0||SGD DESC:DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress Gene:DUT1(YBR252W)|FD-Score:-3.35|P-value:4.04E-4|Clearance:0||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:EFB1(YAL003W)|FD-Score:-4.47|P-value:4.00E-6|Clearance:0||SGD DESC:Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site Gene:ERG11(YHR007C)|FD-Score:-6.25|P-value:2.02E-10|Clearance:0||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ESP1(YGR098C)|FD-Score:-6.95|P-value:1.89E-12|Clearance:0||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:FAS1(YKL182W)|FD-Score:-3.42|P-value:3.14E-4|Clearance:0||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:FAS2(YPL231W)|FD-Score:-7.87|P-value:1.79E-15|Clearance:0||SGD DESC:Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities Gene:GCD2(YGR083C)|FD-Score:-3.76|P-value:8.37E-5|Clearance:0||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GLC7(YER133W)|FD-Score:-11.4|P-value:1.78E-30|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GLN4(YOR168W)|FD-Score:-5.15|P-value:1.32E-7|Clearance:0||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:GPI11(YDR302W)|FD-Score:-4.08|P-value:2.22E-5|Clearance:0||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:GPM1(YKL152C)|FD-Score:3.24|P-value:5.94E-4|Clearance:0.05||SGD DESC:Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis Gene:HRR25(YPL204W)|FD-Score:-4.99|P-value:3.08E-7|Clearance:0||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:HSH49(YOR319W)|FD-Score:-5.43|P-value:2.78E-8|Clearance:0||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:IFH1(YLR223C)|FD-Score:3.65|P-value:1.32E-4|Clearance:0.06||SGD DESC:Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing Gene:ILS1(YBL076C)|FD-Score:-3.9|P-value:4.87E-5|Clearance:0||SGD DESC:Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A Gene:LCB2(YDR062W)|FD-Score:7.72|P-value:5.84E-15|Clearance:1.27||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:LCP5(YER127W)|FD-Score:-5.54|P-value:1.55E-8|Clearance:0||SGD DESC:Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus Gene:LSM5(YER146W)|FD-Score:-3.51|P-value:2.24E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MCM10(YIL150C)|FD-Score:-4.1|P-value:2.09E-5|Clearance:0||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:MED7(YOL135C)|FD-Score:-4.02|P-value:2.90E-5|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MEX67(YPL169C)|FD-Score:-4.97|P-value:3.38E-7|Clearance:0||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:MIM1(YOL026C)|FD-Score:-8.29|P-value:5.59E-17|Clearance:0||SGD DESC:Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex Gene:MMS21(YEL019C)|FD-Score:-7.34|P-value:1.09E-13|Clearance:0||SGD DESC:SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination Gene:MRS6(YOR370C)|FD-Score:-7.89|P-value:1.56E-15|Clearance:0||SGD DESC:Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress Gene:NDC1(YML031W)|FD-Score:-3.5|P-value:2.31E-4|Clearance:0||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:NIP1(YMR309C)|FD-Score:-6.57|P-value:2.60E-11|Clearance:0||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NOP58(YOR310C)|FD-Score:-3.96|P-value:3.69E-5|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NUP1(YOR098C)|FD-Score:6.45|P-value:5.54E-11|Clearance:0.85||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153 Gene:ORC1(YML065W)|FD-Score:-3.42|P-value:3.19E-4|Clearance:0||SGD DESC:Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity Gene:ORC4(YPR162C)|FD-Score:3.58|P-value:1.69E-4|Clearance:0.01||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:ORC6(YHR118C)|FD-Score:-5.27|P-value:6.92E-8|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p Gene:PGA2(YNL149C)|FD-Score:-6.82|P-value:4.68E-12|Clearance:0||SGD DESC:Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect Gene:PRE10(YOR362C)|FD-Score:-3.95|P-value:3.95E-5|Clearance:0||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE2(YPR103W)|FD-Score:-3.14|P-value:8.43E-4|Clearance:0||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:PRE5(YMR314W)|FD-Score:-4.75|P-value:9.93E-7|Clearance:0||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE6(YOL038W)|FD-Score:-9.75|P-value:8.99E-23|Clearance:0||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRP24(YMR268C)|FD-Score:-9.48|P-value:1.30E-21|Clearance:0||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:PRP39(YML046W)|FD-Score:-5.53|P-value:1.60E-8|Clearance:0||SGD DESC:U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats Gene:PRP45(YAL032C)|FD-Score:-3.4|P-value:3.39E-4|Clearance:0||SGD DESC:Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene Gene:PRP9(YDL030W)|FD-Score:-4.28|P-value:9.34E-6|Clearance:0||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex Gene:PUP1(YOR157C)|FD-Score:-6.68|P-value:1.16E-11|Clearance:0||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:PUP2(YGR253C)|FD-Score:-3.17|P-value:7.56E-4|Clearance:0||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:PUP3(YER094C)|FD-Score:-4.25|P-value:1.09E-5|Clearance:0||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:RAD3(YER171W)|FD-Score:-5.06|P-value:2.15E-7|Clearance:0||SGD DESC:5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress Gene:RET3(YPL010W)|FD-Score:3.2|P-value:6.98E-4|Clearance:0.05||SGD DESC:Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RNA14(YMR061W)|FD-Score:-4.66|P-value:1.59E-6|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RNA15(YGL044C)|FD-Score:-6.78|P-value:6.18E-12|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPB5(YBR154C)|FD-Score:-5.35|P-value:4.29E-8|Clearance:0||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPB8(YOR224C)|FD-Score:-3.9|P-value:4.83E-5|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPN11(YFR004W)|FD-Score:-3.11|P-value:9.50E-4|Clearance:0||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RPO26(YPR187W)|FD-Score:-3.45|P-value:2.80E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPP1(YHR062C)|FD-Score:-5.63|P-value:9.14E-9|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS13(YDR064W)|FD-Score:-3.1|P-value:9.58E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPS15(YOL040C)|FD-Score:-5.25|P-value:7.72E-8|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPS31(YLR167W)|FD-Score:-11.3|P-value:5.74E-30|Clearance:0||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:RPS5(YJR123W)|FD-Score:-7.72|P-value:5.81E-15|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:RRP43(YCR035C)|FD-Score:-3.42|P-value:3.18E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:RTT105(YER104W)|FD-Score:-6.89|P-value:2.75E-12|Clearance:0||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SAM50(YNL026W)|FD-Score:-3.26|P-value:5.49E-4|Clearance:0||SGD DESC:Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 Gene:SAS10(YDL153C)|FD-Score:-5.23|P-value:8.59E-8|Clearance:0||SGD DESC:Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance Gene:SDA1(YGR245C)|FD-Score:-10.6|P-value:1.26E-26|Clearance:0||SGD DESC:Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis Gene:SEC21(YNL287W)|FD-Score:3.15|P-value:8.25E-4|Clearance:0.29||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SEC26(YDR238C)|FD-Score:5.6|P-value:1.09E-8|Clearance:0.35||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SEC31(YDL195W)|FD-Score:-4.83|P-value:6.86E-7|Clearance:0||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEC62(YPL094C)|FD-Score:3.43|P-value:3.02E-4|Clearance:0.1||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SES1(YDR023W)|FD-Score:-4.39|P-value:5.69E-6|Clearance:0||SGD DESC:Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p Gene:SKP1(YDR328C)|FD-Score:-3.42|P-value:3.17E-4|Clearance:0||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:SMC5(YOL034W)|FD-Score:-6.14|P-value:4.23E-10|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair Gene:SMD1(YGR074W)|FD-Score:-4.02|P-value:2.94E-5|Clearance:0||SGD DESC:Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress Gene:SNM1(YDR478W)|FD-Score:-4.19|P-value:1.41E-5|Clearance:0||SGD DESC:Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP Gene:SNU56(YDR240C)|FD-Score:3.7|P-value:1.10E-4|Clearance:0.05||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex Gene:SOF1(YLL011W)|FD-Score:-11.8|P-value:2.32E-32|Clearance:0||SGD DESC:Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p Gene:SPC42(YKL042W)|FD-Score:-7.15|P-value:4.42E-13|Clearance:0||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:SPT14(YPL175W)|FD-Score:3.57|P-value:1.76E-4|Clearance:0.14||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:SRB4(YER022W)|FD-Score:-4.49|P-value:3.54E-6|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination Gene:STU2(YLR045C)|FD-Score:-3.24|P-value:5.96E-4|Clearance:0||SGD DESC:Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p Gene:SUI1(YNL244C)|FD-Score:-6.53|P-value:3.26E-11|Clearance:0||SGD DESC:Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase Gene:THS1(YIL078W)|FD-Score:-9.47|P-value:1.42E-21|Clearance:0||SGD DESC:Threonyl-tRNA synthetase, essential cytoplasmic protein Gene:TIM17(YJL143W)|FD-Score:-3.35|P-value:4.04E-4|Clearance:0||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TOM22(YNL131W)|FD-Score:-3.68|P-value:1.15E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:TRS130(YMR218C)|FD-Score:-5|P-value:2.84E-7|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy Gene:TRS31(YDR472W)|FD-Score:-6.66|P-value:1.38E-11|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TSR1(YDL060W)|FD-Score:-4.72|P-value:1.15E-6|Clearance:0||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:TTI1(YKL033W)|FD-Score:-5.26|P-value:7.32E-8|Clearance:0||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies Gene:UTP14(YML093W)|FD-Score:-7.47|P-value:3.87E-14|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:UTP15(YMR093W)|FD-Score:-6.79|P-value:5.45E-12|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTP5(YDR398W)|FD-Score:-5.03|P-value:2.41E-7|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:WRS1(YOL097C)|FD-Score:-6.01|P-value:8.99E-10|Clearance:0||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YGL069C(YGL069C_d)|FD-Score:5.24|P-value:7.86E-8|Clearance:0.36||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance Gene:YGL074C(YGL074C_d)|FD-Score:-13.6|P-value:1.25E-42|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor Gene:YGR073C(YGR073C_d)|FD-Score:-4.58|P-value:2.34E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein Gene:YKL153W(YKL153W_d)|FD-Score:-4.66|P-value:1.57E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant Gene:YLR140W(YLR140W_d)|FD-Score:4.88|P-value:5.32E-7|Clearance:0.95||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I Gene:YLR317W(YLR317W_d)|FD-Score:3.93|P-value:4.24E-5|Clearance:0.23||SGD DESC:Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C Gene:YNL114C(YNL114C_d)|FD-Score:-3.21|P-value:6.62E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YPL251W(YPL251W_d)|FD-Score:-3.99|P-value:3.35E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 771486
Download HOP data (tab-delimited text)  (excel)
Gene:ADH4(YGL256W)|FD-Score:-3.52|P-value:2.13E-4||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:AFI1(YOR129C)|FD-Score:4.82|P-value:7.09E-7||SGD DESC:Arf3p polarization-specific docking factor, required for the polarized distribution of the ADP-ribosylation factor, Arf3p; participates in polarity development and maintenance of a normal haploid budding pattern; interacts with Cnm7p Gene:AFT1(YGL071W)|FD-Score:-5.2|P-value:9.72E-8||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:APM3(YBR288C)|FD-Score:-3.68|P-value:1.19E-4||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:AQY2(YLL052C)|FD-Score:-3.14|P-value:8.45E-4||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:ARA1(YBR149W)|FD-Score:3.77|P-value:8.22E-5||SGD DESC:NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product Gene:ARO7(YPR060C)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ATG22(YCL038C)|FD-Score:4.76|P-value:9.75E-7||SGD DESC:Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation Gene:BER1(YLR412W)|FD-Score:4.95|P-value:3.78E-7||SGD DESC:Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene Gene:BIM1(YER016W)|FD-Score:5.03|P-value:2.45E-7||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:BTS1(YPL069C)|FD-Score:3.78|P-value:7.78E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUB3(YOR026W)|FD-Score:-3.23|P-value:6.21E-4||SGD DESC:Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p Gene:CBP2(YHL038C)|FD-Score:-3.88|P-value:5.26E-5||SGD DESC:Required for splicing of the group I intron bI5 of the COB pre-mRNA; nuclear-encoded mitochondrial protein that binds to the RNA to promote splicing; also involved in but not essential for splicing of the COB bI2 intron and the intron in the 21S rRNA gene Gene:CDC50(YCR094W)|FD-Score:5.09|P-value:1.78E-7||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CNM67(YNL225C)|FD-Score:-3.77|P-value:8.16E-5||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:CSR1(YLR380W)|FD-Score:3.9|P-value:4.75E-5||SGD DESC:Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress Gene:CTK1(YKL139W)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:DAL2(YIR029W)|FD-Score:-3.18|P-value:7.47E-4||SGD DESC:Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DPH6(YLR143W_p)|FD-Score:-3.92|P-value:4.35E-5||SGD DESC:Diphthamide synthetase; catalyzes the last amidation step of diphthamide biosynthesis using ammonium and ATP; evolutionarily conserved in eukaryotes; dph6 mutants exhibit diphthine accumulation and resistance to sordarin, which is indicative of defects in diphthamide formation on EF2; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; DPH6/YLR143W is not an essential gene Gene:DRS2(YAL026C)|FD-Score:7.92|P-value:1.19E-15||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:DSE3(YOR264W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DUG1(YFR044C)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:EBS1(YDR206W)|FD-Score:-3.72|P-value:9.91E-5||SGD DESC:Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors Gene:ECM33(YBR078W)|FD-Score:5.9|P-value:1.77E-9||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ELP6(YMR312W)|FD-Score:-5.53|P-value:1.61E-8||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:EPS1(YIL005W)|FD-Score:3.79|P-value:7.39E-5||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:FAR7(YFR008W)|FD-Score:3.85|P-value:5.80E-5||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FKS1(YLR342W)|FD-Score:-4.35|P-value:6.96E-6||SGD DESC:Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling Gene:FUN14(YAL008W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Mitochondrial protein of unknown function Gene:FUN26(YAL022C)|FD-Score:4.46|P-value:4.01E-6||SGD DESC:Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis Gene:GCS1(YDL226C)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GDH1(YOR375C)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication Gene:GDH2(YDL215C)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:NAD(+)-dependent glutamate dehydrogenase, degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels Gene:GRX3(YDR098C)|FD-Score:5.34|P-value:4.76E-8||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; evidence exists indicating that the translation start site is not Met1 as currently annotated, but rather Met36 Gene:GTT2(YLL060C)|FD-Score:-4.55|P-value:2.70E-6||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:HOP2(YGL033W)|FD-Score:4.36|P-value:6.59E-6||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:HTA1(YDR225W)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IST2(YBR086C)|FD-Score:-3.7|P-value:1.07E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process Gene:KAP120(YPL125W)|FD-Score:-3.1|P-value:9.64E-4||SGD DESC:Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p Gene:LDB19(YOR322C)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:MEF2(YJL102W)|FD-Score:-8.78|P-value:7.86E-19||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MHO1(YJR008W)|FD-Score:5.54|P-value:1.51E-8||SGD DESC:Protein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility Gene:MHT1(YLL062C)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:S-methylmethionine-homocysteine methyltransferase, functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio Gene:MRC1(YCL061C)|FD-Score:-4.57|P-value:2.38E-6||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:MSC3(YLR219W)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress Gene:MTC3(YGL226W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:MVB12(YGR206W)|FD-Score:5.09|P-value:1.82E-7||SGD DESC:ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin Gene:NAP1(YKR048C)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:NMA111(YNL123W)|FD-Score:3.14|P-value:8.39E-4||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NPT1(YOR209C)|FD-Score:5.02|P-value:2.59E-7||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:NUR1(YDL089W)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:ORM2(YLR350W)|FD-Score:5.28|P-value:6.31E-8||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:OXR1(YPL196W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes Gene:PAU13(YHL046C)|FD-Score:-3.47|P-value:2.58E-4||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock Gene:PDX1(YGR193C)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core Gene:PEP7(YDR323C)|FD-Score:4.37|P-value:6.11E-6||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PHO84(YML123C)|FD-Score:-9.5|P-value:1.00E-21||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PST1(YDR055W)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 Gene:PTC1(YDL006W)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PUT2(YHR037W)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism Gene:RAD14(YMR201C)|FD-Score:6.1|P-value:5.43E-10||SGD DESC:Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein Gene:RAS1(YOR101W)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress Gene:RCY1(YJL204C)|FD-Score:6.51|P-value:3.83E-11||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RGA1(YOR127W)|FD-Score:-3.15|P-value:8.14E-4||SGD DESC:GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RPL35B(YDL136W)|FD-Score:-3.17|P-value:7.68E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPO41(YFL036W)|FD-Score:6.47|P-value:4.86E-11||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RTC4(YNL254C)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:RTC6(YPL183W-A)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:RUP1(YOR138C)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SHC1(YER096W)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication Gene:SIR1(YKR101W)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin Gene:SNO4(YMR322C_p)|FD-Score:-4.27|P-value:9.59E-6||SGD DESC:Possible chaperone and cysteine protease, similar to bacterial Hsp31 and yeast Hsp31p, Hsp32p, and Hsp33p; DJ-1/ThiJ/PfpI superfamily member; predicted involvement in pyridoxine metabolism; induced by mild heat stress and copper deprivation Gene:SNX4(YJL036W)|FD-Score:-3.15|P-value:8.10E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPL2(YHR136C)|FD-Score:5.98|P-value:1.09E-9||SGD DESC:Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm Gene:SSN2(YDR443C)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SSP1(YHR184W)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Protein involved in the control of meiotic nuclear division and coordination of meiosis with spore formation; transcription is induced midway through meiosis Gene:SWP82(YFL049W)|FD-Score:-4|P-value:3.12E-5||SGD DESC:Member of the SWI/SNF chromatin remodeling complex; has an as yet unidentified role in the complex; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p; relocates to the cytosol under hypoxic conditions Gene:SYN8(YAL014C)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Endosomal SNARE related to mammalian syntaxin 8 Gene:THI22(YPR121W)|FD-Score:3.25|P-value:5.81E-4||SGD DESC:Protein with similarity to hydroxymethylpyrimidine phosphate kinases; member of a gene family with THI20 and THI21; not required for thiamine biosynthesis Gene:TPO1(YLL028W)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane Gene:UBC8(YEL012W)|FD-Score:5.53|P-value:1.60E-8||SGD DESC:Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro Gene:UBR2(YLR024C)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex Gene:VTA1(YLR181C)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Multivesicular body (MVB) protein involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain Gene:YBL055C(YBL055C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YCR050C(YCR050C_p)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YDL034W(YDL034W_d)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene Gene:YER034W(YER034W_p)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YHR180W(YHR180W_d)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL024C(YIL024C_p)|FD-Score:4.5|P-value:3.42E-6||SGD DESC:Putative protein of unknown function; non-essential gene; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p Gene:YIL092W(YIL092W_p)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YJL027C(YJL027C_p)|FD-Score:3.79|P-value:7.41E-5||SGD DESC:Putative protein of unknown function Gene:YKR011C(YKR011C_p)|FD-Score:-3.29|P-value:5.00E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress Gene:YKU70(YMR284W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair Gene:YLR012C(YLR012C_p)|FD-Score:3.13|P-value:8.89E-4||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YLR063W(YLR063W_p)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene Gene:YLR072W(YLR072W_p)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene Gene:YLR252W(YLR252W_d)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism Gene:YMR074C(YMR074C)|FD-Score:5.14|P-value:1.39E-7||SGD DESC:Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YMR242W-A(YMR242W-A_p)|FD-Score:-4.52|P-value:3.06E-6||SGD DESC:Putative protein of unknown function Gene:YMR304C-A(YMR304C-A_d)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10 Gene:YNL057W(YNL057W_d)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL153C(YOL153C)|FD-Score:-3.17|P-value:7.63E-4||SGD DESC:Hypothetical protein Gene:YPL073C(YPL073C_d)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Gene:ADH4(YGL256W)|FD-Score:-3.52|P-value:2.13E-4||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:AFI1(YOR129C)|FD-Score:4.82|P-value:7.09E-7||SGD DESC:Arf3p polarization-specific docking factor, required for the polarized distribution of the ADP-ribosylation factor, Arf3p; participates in polarity development and maintenance of a normal haploid budding pattern; interacts with Cnm7p Gene:AFT1(YGL071W)|FD-Score:-5.2|P-value:9.72E-8||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:APM3(YBR288C)|FD-Score:-3.68|P-value:1.19E-4||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:AQY2(YLL052C)|FD-Score:-3.14|P-value:8.45E-4||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:ARA1(YBR149W)|FD-Score:3.77|P-value:8.22E-5||SGD DESC:NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product Gene:ARO7(YPR060C)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ATG22(YCL038C)|FD-Score:4.76|P-value:9.75E-7||SGD DESC:Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation Gene:BER1(YLR412W)|FD-Score:4.95|P-value:3.78E-7||SGD DESC:Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene Gene:BIM1(YER016W)|FD-Score:5.03|P-value:2.45E-7||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:BTS1(YPL069C)|FD-Score:3.78|P-value:7.78E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUB3(YOR026W)|FD-Score:-3.23|P-value:6.21E-4||SGD DESC:Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p Gene:CBP2(YHL038C)|FD-Score:-3.88|P-value:5.26E-5||SGD DESC:Required for splicing of the group I intron bI5 of the COB pre-mRNA; nuclear-encoded mitochondrial protein that binds to the RNA to promote splicing; also involved in but not essential for splicing of the COB bI2 intron and the intron in the 21S rRNA gene Gene:CDC50(YCR094W)|FD-Score:5.09|P-value:1.78E-7||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CNM67(YNL225C)|FD-Score:-3.77|P-value:8.16E-5||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:CSR1(YLR380W)|FD-Score:3.9|P-value:4.75E-5||SGD DESC:Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress Gene:CTK1(YKL139W)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:DAL2(YIR029W)|FD-Score:-3.18|P-value:7.47E-4||SGD DESC:Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DPH6(YLR143W_p)|FD-Score:-3.92|P-value:4.35E-5||SGD DESC:Diphthamide synthetase; catalyzes the last amidation step of diphthamide biosynthesis using ammonium and ATP; evolutionarily conserved in eukaryotes; dph6 mutants exhibit diphthine accumulation and resistance to sordarin, which is indicative of defects in diphthamide formation on EF2; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; DPH6/YLR143W is not an essential gene Gene:DRS2(YAL026C)|FD-Score:7.92|P-value:1.19E-15||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:DSE3(YOR264W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DUG1(YFR044C)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:EBS1(YDR206W)|FD-Score:-3.72|P-value:9.91E-5||SGD DESC:Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors Gene:ECM33(YBR078W)|FD-Score:5.9|P-value:1.77E-9||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ELP6(YMR312W)|FD-Score:-5.53|P-value:1.61E-8||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:EPS1(YIL005W)|FD-Score:3.79|P-value:7.39E-5||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:FAR7(YFR008W)|FD-Score:3.85|P-value:5.80E-5||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FKS1(YLR342W)|FD-Score:-4.35|P-value:6.96E-6||SGD DESC:Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling Gene:FUN14(YAL008W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Mitochondrial protein of unknown function Gene:FUN26(YAL022C)|FD-Score:4.46|P-value:4.01E-6||SGD DESC:Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis Gene:GCS1(YDL226C)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GDH1(YOR375C)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication Gene:GDH2(YDL215C)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:NAD(+)-dependent glutamate dehydrogenase, degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels Gene:GRX3(YDR098C)|FD-Score:5.34|P-value:4.76E-8||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; evidence exists indicating that the translation start site is not Met1 as currently annotated, but rather Met36 Gene:GTT2(YLL060C)|FD-Score:-4.55|P-value:2.70E-6||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:HOP2(YGL033W)|FD-Score:4.36|P-value:6.59E-6||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:HTA1(YDR225W)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IST2(YBR086C)|FD-Score:-3.7|P-value:1.07E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process Gene:KAP120(YPL125W)|FD-Score:-3.1|P-value:9.64E-4||SGD DESC:Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p Gene:LDB19(YOR322C)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:MEF2(YJL102W)|FD-Score:-8.78|P-value:7.86E-19||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MHO1(YJR008W)|FD-Score:5.54|P-value:1.51E-8||SGD DESC:Protein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility Gene:MHT1(YLL062C)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:S-methylmethionine-homocysteine methyltransferase, functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio Gene:MRC1(YCL061C)|FD-Score:-4.57|P-value:2.38E-6||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:MSC3(YLR219W)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress Gene:MTC3(YGL226W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:MVB12(YGR206W)|FD-Score:5.09|P-value:1.82E-7||SGD DESC:ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin Gene:NAP1(YKR048C)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:NMA111(YNL123W)|FD-Score:3.14|P-value:8.39E-4||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NPT1(YOR209C)|FD-Score:5.02|P-value:2.59E-7||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:NUR1(YDL089W)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:ORM2(YLR350W)|FD-Score:5.28|P-value:6.31E-8||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:OXR1(YPL196W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes Gene:PAU13(YHL046C)|FD-Score:-3.47|P-value:2.58E-4||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock Gene:PDX1(YGR193C)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core Gene:PEP7(YDR323C)|FD-Score:4.37|P-value:6.11E-6||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PHO84(YML123C)|FD-Score:-9.5|P-value:1.00E-21||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PST1(YDR055W)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 Gene:PTC1(YDL006W)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PUT2(YHR037W)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism Gene:RAD14(YMR201C)|FD-Score:6.1|P-value:5.43E-10||SGD DESC:Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein Gene:RAS1(YOR101W)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress Gene:RCY1(YJL204C)|FD-Score:6.51|P-value:3.83E-11||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RGA1(YOR127W)|FD-Score:-3.15|P-value:8.14E-4||SGD DESC:GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RPL35B(YDL136W)|FD-Score:-3.17|P-value:7.68E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPO41(YFL036W)|FD-Score:6.47|P-value:4.86E-11||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RTC4(YNL254C)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:RTC6(YPL183W-A)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:RUP1(YOR138C)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SHC1(YER096W)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication Gene:SIR1(YKR101W)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin Gene:SNO4(YMR322C_p)|FD-Score:-4.27|P-value:9.59E-6||SGD DESC:Possible chaperone and cysteine protease, similar to bacterial Hsp31 and yeast Hsp31p, Hsp32p, and Hsp33p; DJ-1/ThiJ/PfpI superfamily member; predicted involvement in pyridoxine metabolism; induced by mild heat stress and copper deprivation Gene:SNX4(YJL036W)|FD-Score:-3.15|P-value:8.10E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPL2(YHR136C)|FD-Score:5.98|P-value:1.09E-9||SGD DESC:Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm Gene:SSN2(YDR443C)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SSP1(YHR184W)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Protein involved in the control of meiotic nuclear division and coordination of meiosis with spore formation; transcription is induced midway through meiosis Gene:SWP82(YFL049W)|FD-Score:-4|P-value:3.12E-5||SGD DESC:Member of the SWI/SNF chromatin remodeling complex; has an as yet unidentified role in the complex; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p; relocates to the cytosol under hypoxic conditions Gene:SYN8(YAL014C)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Endosomal SNARE related to mammalian syntaxin 8 Gene:THI22(YPR121W)|FD-Score:3.25|P-value:5.81E-4||SGD DESC:Protein with similarity to hydroxymethylpyrimidine phosphate kinases; member of a gene family with THI20 and THI21; not required for thiamine biosynthesis Gene:TPO1(YLL028W)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane Gene:UBC8(YEL012W)|FD-Score:5.53|P-value:1.60E-8||SGD DESC:Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro Gene:UBR2(YLR024C)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex Gene:VTA1(YLR181C)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Multivesicular body (MVB) protein involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain Gene:YBL055C(YBL055C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YCR050C(YCR050C_p)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YDL034W(YDL034W_d)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene Gene:YER034W(YER034W_p)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YHR180W(YHR180W_d)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL024C(YIL024C_p)|FD-Score:4.5|P-value:3.42E-6||SGD DESC:Putative protein of unknown function; non-essential gene; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p Gene:YIL092W(YIL092W_p)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YJL027C(YJL027C_p)|FD-Score:3.79|P-value:7.41E-5||SGD DESC:Putative protein of unknown function Gene:YKR011C(YKR011C_p)|FD-Score:-3.29|P-value:5.00E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress Gene:YKU70(YMR284W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair Gene:YLR012C(YLR012C_p)|FD-Score:3.13|P-value:8.89E-4||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YLR063W(YLR063W_p)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene Gene:YLR072W(YLR072W_p)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene Gene:YLR252W(YLR252W_d)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism Gene:YMR074C(YMR074C)|FD-Score:5.14|P-value:1.39E-7||SGD DESC:Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YMR242W-A(YMR242W-A_p)|FD-Score:-4.52|P-value:3.06E-6||SGD DESC:Putative protein of unknown function Gene:YMR304C-A(YMR304C-A_d)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10 Gene:YNL057W(YNL057W_d)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL153C(YOL153C)|FD-Score:-3.17|P-value:7.63E-4||SGD DESC:Hypothetical protein Gene:YPL073C(YPL073C_d)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR062W7.725.84E-151.27LCB2Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
YOR098C6.455.54E-110.85NUP1FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153
YDR238C5.601.09E-80.35SEC26Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
YGL069C_d5.247.86E-80.36YGL069C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance
YLR140W_d4.885.32E-70.95YLR140W_dDubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I
YLR317W_d3.934.24E-50.23YLR317W_dDubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C
YDR240C3.701.10E-40.05SNU56Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex
YLR223C3.651.32E-40.06IFH1Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing
YPR162C3.581.69E-40.01ORC4Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
YPL175W3.571.76E-40.14SPT14UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins
YPL094C3.433.02E-40.10SEC62Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YKL192C3.334.41E-40.08ACP1Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p
YKL152C3.245.94E-40.05GPM1Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis
YPL010W3.206.98E-40.05RET3Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
YNL287W3.158.25E-40.29SEC21Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YAL026C7.921.19E-15DRS2Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease
YJL204C6.513.83E-11RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YFL036W6.474.86E-11RPO41Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
YMR201C6.105.43E-10RAD14Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein
YHR136C5.981.09E-9SPL2Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm
YBR078W5.901.77E-9ECM33GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p
YJR008W5.541.51E-8MHO1Protein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility
YEL012W5.531.60E-8UBC8Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro
YDR098C5.344.76E-8GRX3Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; evidence exists indicating that the translation start site is not Met1 as currently annotated, but rather Met36
YLR350W5.286.31E-8ORM2Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress
YMR074C5.141.39E-7YMR074CProtein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress
YCR094W5.091.78E-7CDC50Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
YGR206W5.091.82E-7MVB12ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
YER016W5.032.45E-7BIM1Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally
YOR209C5.022.59E-7NPT1Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus

GO enrichment analysis for SGTC_372
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1963.37E-51SGTC_7783456-2434 196.0 μMChemDiv (Drug-like library)55390630.12069mitochondrial response to ROS
0.1762.14E-41SGTC_30629077494 49.5 μMChembridge (Drug-like library)164596330.0746269mitochondrial response to ROS
0.1691.81E-38SGTC_13531498-1206 8.1 μMChemDiv (Drug-like library)67568080.192308mitochondrial response to ROS
0.1581.07E-33SGTC_1160155-0182 31.8 μMChemDiv (Drug-like library)7714861mitochondrial response to ROS
0.1551.54E-32SGTC_490phenamil 164.0 μMMiscellaneous47550.0793651heme requiring
0.1521.76E-31SGTC_29659082772 38.6 μMChembridge (Drug-like library)164596340.0869565mitochondrial response to ROS
0.1522.66E-31SGTC_1410443-0274 32.3 μMChemDiv (Drug-like library)16272410.222222mitochondrial response to ROS
0.1501.55E-30SGTC_3650121-0052 13.6 μMChemDiv (Drug-like library)31017390.0967742mitochondrial response to ROS
0.1371.04E-25SGTC_29259010536 32.5 μMChembridge (Drug-like library)29941770.0833333azole & statin
0.1353.68E-25SGTC_20545233173 129.0 μMChembridge (Fragment library)68890.0588235mitochondrial response to ROS
0.1267.86E-22SGTC_5471120-0019 610.0 nMChemDiv (Drug-like library)36769390.0875azole & statin
0.1242.32E-21SGTC_3832324-0395 7.4 μMChemDiv (Drug-like library)31464210.1azole & statin
0.1121.46E-17SGTC_13471493-0251 600.0 nMChemDiv (Drug-like library)39863780.261905mitochondrial response to ROS
0.1105.04E-17SGTC_1036k216-0215 33.5 μMChemDiv (Drug-like library)6223300.0909091mitochondrial response to ROS
0.1098.31E-17SGTC_2739tacrine 100.9 μMMiscellaneous19350.0943396heme requiring

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1160155-018231.79 μM1771486ChemDiv (Drug-like library)241.308283.24322mitochondrial response to ROS
SGTC_630099-024226.7 μM0.3333333898502ChemDiv (Drug-like library)265.372744.0501mitochondrial stress
SGTC_2566crystal violet carbinol226 nM0.32432468050Miscellaneous389.533144.76314
SGTC_774k060-0086136 μM0.3018875911235ChemDiv (Drug-like library)280.324443.67815
SGTC_2042p-Dimethylaminoacetophenone200 μM0.28205175037Chembridge (Fragment library)163.216321.73202
SGTC_6390833-0042114 μM0.2692315978911ChemDiv (Drug-like library)252.311042.71303
SGTC_13471493-0251600 nM0.2619053986378ChemDiv (Drug-like library)438.583884.99803mitochondrial response to ROS
SGTC_7334069-000756.1 μM0.2592595717425ChemDiv (Drug-like library)309.193764.32814ERAD & cell cycle
SGTC_15852',4'-dihydroxychalcone10.4 μM0.2553195357218TimTec (Pure natural product library)240.253983.21823
SGTC_25732',4'-dihydroxychalcone15.04 μM0.2553195357218TimTec (Pure natural product library)240.253983.21823
SGTC_25742',4'-dihydroxychalcone14.64 μM0.2553195357218TimTec (Pure natural product library)240.253983.21823
SGTC_26602',4'-dihydroxychalcone17.14 μM0.2553195357218TimTec (Pure natural product library)240.253983.21823
SGTC_26682',4'-dihydroxychalcone15.08 μM0.2553195357218TimTec (Pure natural product library)240.253983.21823
SGTC_11730443-0020122 μM0.2549025331295ChemDiv (Drug-like library)254.280563.44313TSC3-RPN4