3970-0862

7-(hydroxymethyl)-2-(4-methyl-2-nitrophenyl)-4,7a-dihydro-3aH-octahydro-1H-4,7-epoxyisoindole-1,3-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_376
Screen concentration 59.1 μM
Source ChemDiv (Drug-like library)
PubChem CID 3135186
SMILES CC1=CC(=C(C=C1)N2C(=O)C3C4C=CC(C3C2=O)(O4)CO)[N+](=O)[O-]
Standardized SMILES Cc1ccc(N2C(=O)C3C4OC(CO)(C=C4)C3C2=O)c(c1)[N+](=O)[O-]
Molecular weight 330.2922
ALogP 0.54
H-bond donor count 1
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 34.49
% growth inhibition (Hom. pool) 20.3


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3135186
Download HIP data (tab-delimited text)  (excel)
Gene:ALG1(YBR110W)|FD-Score:4.15|P-value:1.63E-5|Clearance:0.02||SGD DESC:Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog Gene:DML1(YMR211W)|FD-Score:7.81|P-value:2.89E-15|Clearance:1.76||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:DNA2(YHR164C)|FD-Score:3.1|P-value:9.63E-4|Clearance:0.01||SGD DESC:Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress Gene:ECM9(YKR004C)|FD-Score:3.72|P-value:1.01E-4|Clearance:0.17||SGD DESC:Non-essential protein of unknown function Gene:EMG1(YLR186W)|FD-Score:-3.32|P-value:4.43E-4|Clearance:0||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:ERG11(YHR007C)|FD-Score:4.33|P-value:7.54E-6|Clearance:0.1||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:GPI14(YJR013W)|FD-Score:3.21|P-value:6.62E-4|Clearance:0.02||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:GPI8(YDR331W)|FD-Score:3.35|P-value:4.06E-4|Clearance:0.1||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:HSH49(YOR319W)|FD-Score:3.77|P-value:8.07E-5|Clearance:0.06||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:KAP95(YLR347C)|FD-Score:4.45|P-value:4.31E-6|Clearance:0.12||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:KRE29(YER038C)|FD-Score:3.25|P-value:5.73E-4|Clearance:0.04||SGD DESC:Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:LCB2(YDR062W)|FD-Score:4.23|P-value:1.18E-5|Clearance:0.07||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:LSM3(YLR438C-A)|FD-Score:3.36|P-value:3.94E-4|Clearance:0.01||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MET30(YIL046W)|FD-Score:-4.87|P-value:5.58E-7|Clearance:0||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:NOP8(YOL144W)|FD-Score:3.19|P-value:7.11E-4|Clearance:0.07||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:ORC4(YPR162C)|FD-Score:-3.55|P-value:1.92E-4|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:RAP1(YNL216W)|FD-Score:3.41|P-value:3.28E-4|Clearance:0.05||SGD DESC:Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) Gene:RPL5(YPL131W)|FD-Score:-4.06|P-value:2.49E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:SGD1(YLR336C)|FD-Score:3.55|P-value:1.96E-4|Clearance:0.09||SGD DESC:Essential nuclear protein, required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia Gene:SHQ1(YIL104C)|FD-Score:7.55|P-value:2.17E-14|Clearance:1.76||SGD DESC:Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones Gene:SKP1(YDR328C)|FD-Score:-3.45|P-value:2.80E-4|Clearance:0||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:SMD1(YGR074W)|FD-Score:5.79|P-value:3.60E-9|Clearance:1.34||SGD DESC:Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress Gene:SQT1(YIR012W)|FD-Score:3.09|P-value:9.89E-4|Clearance:0.02||SGD DESC:Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:SRP1(YNL189W)|FD-Score:3.46|P-value:2.70E-4|Clearance:0.05||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:STS1(YIR011C)|FD-Score:3.79|P-value:7.50E-5|Clearance:0.02||SGD DESC:Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation Gene:TFC1(YBR123C)|FD-Score:3.12|P-value:9.13E-4|Clearance:0.02||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:TIM54(YJL054W)|FD-Score:4.13|P-value:1.78E-5|Clearance:0.34||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:TUB1(YML085C)|FD-Score:-6.75|P-value:7.26E-12|Clearance:0||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YDR327W(YDR327W_d)|FD-Score:-3.69|P-value:1.14E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1 Gene:ALG1(YBR110W)|FD-Score:4.15|P-value:1.63E-5|Clearance:0.02||SGD DESC:Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog Gene:DML1(YMR211W)|FD-Score:7.81|P-value:2.89E-15|Clearance:1.76||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:DNA2(YHR164C)|FD-Score:3.1|P-value:9.63E-4|Clearance:0.01||SGD DESC:Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress Gene:ECM9(YKR004C)|FD-Score:3.72|P-value:1.01E-4|Clearance:0.17||SGD DESC:Non-essential protein of unknown function Gene:EMG1(YLR186W)|FD-Score:-3.32|P-value:4.43E-4|Clearance:0||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:ERG11(YHR007C)|FD-Score:4.33|P-value:7.54E-6|Clearance:0.1||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:GPI14(YJR013W)|FD-Score:3.21|P-value:6.62E-4|Clearance:0.02||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:GPI8(YDR331W)|FD-Score:3.35|P-value:4.06E-4|Clearance:0.1||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:HSH49(YOR319W)|FD-Score:3.77|P-value:8.07E-5|Clearance:0.06||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:KAP95(YLR347C)|FD-Score:4.45|P-value:4.31E-6|Clearance:0.12||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:KRE29(YER038C)|FD-Score:3.25|P-value:5.73E-4|Clearance:0.04||SGD DESC:Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:LCB2(YDR062W)|FD-Score:4.23|P-value:1.18E-5|Clearance:0.07||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:LSM3(YLR438C-A)|FD-Score:3.36|P-value:3.94E-4|Clearance:0.01||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MET30(YIL046W)|FD-Score:-4.87|P-value:5.58E-7|Clearance:0||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:NOP8(YOL144W)|FD-Score:3.19|P-value:7.11E-4|Clearance:0.07||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:ORC4(YPR162C)|FD-Score:-3.55|P-value:1.92E-4|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:RAP1(YNL216W)|FD-Score:3.41|P-value:3.28E-4|Clearance:0.05||SGD DESC:Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) Gene:RPL5(YPL131W)|FD-Score:-4.06|P-value:2.49E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:SGD1(YLR336C)|FD-Score:3.55|P-value:1.96E-4|Clearance:0.09||SGD DESC:Essential nuclear protein, required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia Gene:SHQ1(YIL104C)|FD-Score:7.55|P-value:2.17E-14|Clearance:1.76||SGD DESC:Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones Gene:SKP1(YDR328C)|FD-Score:-3.45|P-value:2.80E-4|Clearance:0||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:SMD1(YGR074W)|FD-Score:5.79|P-value:3.60E-9|Clearance:1.34||SGD DESC:Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress Gene:SQT1(YIR012W)|FD-Score:3.09|P-value:9.89E-4|Clearance:0.02||SGD DESC:Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:SRP1(YNL189W)|FD-Score:3.46|P-value:2.70E-4|Clearance:0.05||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:STS1(YIR011C)|FD-Score:3.79|P-value:7.50E-5|Clearance:0.02||SGD DESC:Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation Gene:TFC1(YBR123C)|FD-Score:3.12|P-value:9.13E-4|Clearance:0.02||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:TIM54(YJL054W)|FD-Score:4.13|P-value:1.78E-5|Clearance:0.34||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:TUB1(YML085C)|FD-Score:-6.75|P-value:7.26E-12|Clearance:0||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YDR327W(YDR327W_d)|FD-Score:-3.69|P-value:1.14E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3135186
Download HOP data (tab-delimited text)  (excel)
Gene:BST1(YFL025C)|FD-Score:5.85|P-value:2.46E-9||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BUD7(YOR299W)|FD-Score:-3.29|P-value:5.01E-4||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BUD7 has a paralog, BCH1, that arose from the whole genome duplication Gene:CBR1(YIL043C)|FD-Score:3.12|P-value:9.08E-4||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CKI1(YLR133W)|FD-Score:-6|P-value:1.00E-9||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:CLC1(YGR167W)|FD-Score:5|P-value:2.86E-7||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COX23(YHR116W)|FD-Score:3.8|P-value:7.11E-5||SGD DESC:Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs Gene:CSF1(YLR087C)|FD-Score:4.72|P-value:1.20E-6||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CSG2(YBR036C)|FD-Score:-3.27|P-value:5.41E-4||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:DBP1(YPL119C)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress Gene:DIG2(YDR480W)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DIT1(YDR403W)|FD-Score:-3.54|P-value:2.04E-4||SGD DESC:Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure Gene:ECM33(YBR078W)|FD-Score:5.56|P-value:1.36E-8||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ERG4(YGL012W)|FD-Score:6.68|P-value:1.22E-11||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERV46(YAL042W)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:FIR1(YER032W)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Protein involved in 3' mRNA processing, interacts with Ref2p; potential Cdc28p substrate Gene:FRA1(YLL029W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:GGA1(YDR358W)|FD-Score:5.1|P-value:1.69E-7||SGD DESC:Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Gene:GPB1(YOR371C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication Gene:GSH1(YJL101C)|FD-Score:3.82|P-value:6.61E-5||SGD DESC:Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress Gene:GTB1(YDR221W)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress Gene:GUP1(YGL084C)|FD-Score:5.03|P-value:2.51E-7||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HBT1(YDL223C)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HPA2(YPR193C)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity Gene:IFA38(YBR159W)|FD-Score:4.58|P-value:2.38E-6||SGD DESC:Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides Gene:IZH3(YLR023C)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity Gene:LDB19(YOR322C)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LYS4(YDR234W)|FD-Score:-3.23|P-value:6.12E-4||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MBF1(YOR298C-A)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress Gene:MDM35(YKL053C-A)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MFT1(YML062C)|FD-Score:4.45|P-value:4.22E-6||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MGS1(YNL218W)|FD-Score:-4|P-value:3.17E-5||SGD DESC:Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress Gene:MTM1(YGR257C)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:NIP100(YPL174C)|FD-Score:-3.27|P-value:5.39E-4||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:NOT3(YIL038C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:PAC2(YER007W)|FD-Score:6.66|P-value:1.34E-11||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PAH1(YMR165C)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PDR16(YNL231C)|FD-Score:4.66|P-value:1.58E-6||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PMU1(YKL128C)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Putative phosphomutase, contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant Gene:PRK1(YIL095W)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication Gene:RGL1(YPL066W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RPL1A(YPL220W)|FD-Score:3.79|P-value:7.56E-5||SGD DESC:Ribosomal 60S subunit protein L1A; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal Gene:RPS17A(YML024W)|FD-Score:-3.48|P-value:2.54E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:RPS30A(YLR287C-A)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:RRM3(YHR031C)|FD-Score:-3.23|P-value:6.23E-4||SGD DESC:DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p Gene:SKI8(YGL213C)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype Gene:SMM1(YNR015W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Dihydrouridine synthase, member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs Gene:SPC72(YAL047C)|FD-Score:4.7|P-value:1.33E-6||SGD DESC:Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization Gene:SSK22(YCR073C)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; functionally redundant with, and homologous to, Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p Gene:SWR1(YDR334W)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TSC3(YBR058C-A)|FD-Score:3.84|P-value:6.11E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:6.57|P-value:2.54E-11||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:VPS27(YNR006W)|FD-Score:4.39|P-value:5.63E-6||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS9(YML097C)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:WWM1(YFL010C)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:WW domain containing protein of unknown function; binds to Mca1p, a caspase-related protease that regulates H2O2-induced apoptosis; overexpression causes G1 phase growth arrest and clonal death that is suppressed by overexpression of MCA1 Gene:YAP1(YML007W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YBR056W(YBR056W_p)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YBR113W(YBR113W_d)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8 Gene:YCR022C(YCR022C_d)|FD-Score:-3.24|P-value:6.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene Gene:YEH2(YLR020C)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism Gene:YHR130C(YHR130C_d)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIH1(YCR059C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YKL053W(YKL053W_d)|FD-Score:3.96|P-value:3.69E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 Gene:YKR075C(YKR075C_p)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YLR455W(YLR455W_p)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YML119W(YML119W_p)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate Gene:YMR181C(YMR181C_p)|FD-Score:3.87|P-value:5.46E-5||SGD DESC:Protein of unknown function; mRNA transcribed as part of a bicistronic transcript with a predicted transcriptional repressor RGM1/YMR182C; mRNA is destroyed by nonsense-mediated decay (NMD); not an essential gene; YMR181C has a paralog, YPL229W, that arose from the whole genome duplication Gene:YPL225W(YPL225W)|FD-Score:-3.16|P-value:7.88E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPR071W(YPR071W_p)|FD-Score:4.98|P-value:3.20E-7||SGD DESC:Putative membrane protein; YPR071W is not an essential gene; YPR071W has paralog YIL029C Gene:YPR159C-A(YPR159C-A_p)|FD-Score:3.91|P-value:4.52E-5||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:BST1(YFL025C)|FD-Score:5.85|P-value:2.46E-9||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BUD7(YOR299W)|FD-Score:-3.29|P-value:5.01E-4||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BUD7 has a paralog, BCH1, that arose from the whole genome duplication Gene:CBR1(YIL043C)|FD-Score:3.12|P-value:9.08E-4||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CKI1(YLR133W)|FD-Score:-6|P-value:1.00E-9||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:CLC1(YGR167W)|FD-Score:5|P-value:2.86E-7||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COX23(YHR116W)|FD-Score:3.8|P-value:7.11E-5||SGD DESC:Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs Gene:CSF1(YLR087C)|FD-Score:4.72|P-value:1.20E-6||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CSG2(YBR036C)|FD-Score:-3.27|P-value:5.41E-4||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:DBP1(YPL119C)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress Gene:DIG2(YDR480W)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DIT1(YDR403W)|FD-Score:-3.54|P-value:2.04E-4||SGD DESC:Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure Gene:ECM33(YBR078W)|FD-Score:5.56|P-value:1.36E-8||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ERG4(YGL012W)|FD-Score:6.68|P-value:1.22E-11||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERV46(YAL042W)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:FIR1(YER032W)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Protein involved in 3' mRNA processing, interacts with Ref2p; potential Cdc28p substrate Gene:FRA1(YLL029W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:GGA1(YDR358W)|FD-Score:5.1|P-value:1.69E-7||SGD DESC:Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Gene:GPB1(YOR371C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication Gene:GSH1(YJL101C)|FD-Score:3.82|P-value:6.61E-5||SGD DESC:Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress Gene:GTB1(YDR221W)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress Gene:GUP1(YGL084C)|FD-Score:5.03|P-value:2.51E-7||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HBT1(YDL223C)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HPA2(YPR193C)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity Gene:IFA38(YBR159W)|FD-Score:4.58|P-value:2.38E-6||SGD DESC:Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides Gene:IZH3(YLR023C)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity Gene:LDB19(YOR322C)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LYS4(YDR234W)|FD-Score:-3.23|P-value:6.12E-4||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MBF1(YOR298C-A)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress Gene:MDM35(YKL053C-A)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MFT1(YML062C)|FD-Score:4.45|P-value:4.22E-6||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MGS1(YNL218W)|FD-Score:-4|P-value:3.17E-5||SGD DESC:Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress Gene:MTM1(YGR257C)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:NIP100(YPL174C)|FD-Score:-3.27|P-value:5.39E-4||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:NOT3(YIL038C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:PAC2(YER007W)|FD-Score:6.66|P-value:1.34E-11||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PAH1(YMR165C)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PDR16(YNL231C)|FD-Score:4.66|P-value:1.58E-6||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PMU1(YKL128C)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Putative phosphomutase, contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant Gene:PRK1(YIL095W)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication Gene:RGL1(YPL066W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RPL1A(YPL220W)|FD-Score:3.79|P-value:7.56E-5||SGD DESC:Ribosomal 60S subunit protein L1A; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal Gene:RPS17A(YML024W)|FD-Score:-3.48|P-value:2.54E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:RPS30A(YLR287C-A)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:RRM3(YHR031C)|FD-Score:-3.23|P-value:6.23E-4||SGD DESC:DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p Gene:SKI8(YGL213C)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype Gene:SMM1(YNR015W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Dihydrouridine synthase, member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs Gene:SPC72(YAL047C)|FD-Score:4.7|P-value:1.33E-6||SGD DESC:Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization Gene:SSK22(YCR073C)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; functionally redundant with, and homologous to, Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p Gene:SWR1(YDR334W)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TSC3(YBR058C-A)|FD-Score:3.84|P-value:6.11E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:6.57|P-value:2.54E-11||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:VPS27(YNR006W)|FD-Score:4.39|P-value:5.63E-6||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS9(YML097C)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:WWM1(YFL010C)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:WW domain containing protein of unknown function; binds to Mca1p, a caspase-related protease that regulates H2O2-induced apoptosis; overexpression causes G1 phase growth arrest and clonal death that is suppressed by overexpression of MCA1 Gene:YAP1(YML007W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YBR056W(YBR056W_p)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YBR113W(YBR113W_d)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8 Gene:YCR022C(YCR022C_d)|FD-Score:-3.24|P-value:6.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene Gene:YEH2(YLR020C)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism Gene:YHR130C(YHR130C_d)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIH1(YCR059C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YKL053W(YKL053W_d)|FD-Score:3.96|P-value:3.69E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 Gene:YKR075C(YKR075C_p)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YLR455W(YLR455W_p)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YML119W(YML119W_p)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate Gene:YMR181C(YMR181C_p)|FD-Score:3.87|P-value:5.46E-5||SGD DESC:Protein of unknown function; mRNA transcribed as part of a bicistronic transcript with a predicted transcriptional repressor RGM1/YMR182C; mRNA is destroyed by nonsense-mediated decay (NMD); not an essential gene; YMR181C has a paralog, YPL229W, that arose from the whole genome duplication Gene:YPL225W(YPL225W)|FD-Score:-3.16|P-value:7.88E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPR071W(YPR071W_p)|FD-Score:4.98|P-value:3.20E-7||SGD DESC:Putative membrane protein; YPR071W is not an essential gene; YPR071W has paralog YIL029C Gene:YPR159C-A(YPR159C-A_p)|FD-Score:3.91|P-value:4.52E-5||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR211W7.812.89E-151.76DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YIL104C7.552.17E-141.76SHQ1Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones
YGR074W5.793.60E-91.34SMD1Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress
YLR347C4.454.31E-60.12KAP95Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p
YHR007C4.337.54E-60.10ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YDR062W4.231.18E-50.07LCB2Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
YBR110W4.151.63E-50.02ALG1Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog
YJL054W4.131.78E-50.34TIM54Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane
YIR011C3.797.50E-50.02STS1Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation
YOR319W3.778.07E-50.06HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YKR004C3.721.01E-40.17ECM9Non-essential protein of unknown function
YLR336C3.551.96E-40.09SGD1Essential nuclear protein, required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia
YNL189W3.462.70E-40.05SRP1Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation
YNL216W3.413.28E-40.05RAP1Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain)
YLR438C-A3.363.94E-40.01LSM3Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL012W6.681.22E-11ERG4C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
YER007W6.661.34E-11PAC2Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
YML124C6.572.54E-11TUB3Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
YFL025C5.852.46E-9BST1GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules
YBR078W5.561.36E-8ECM33GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p
YDR358W5.101.69E-7GGA1Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi
YGL084C5.032.51E-7GUP1Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport
YGR167W5.002.86E-7CLC1Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W
YPR071W_p4.983.20E-7YPR071W_pPutative membrane protein; YPR071W is not an essential gene; YPR071W has paralog YIL029C
YLR087C4.721.20E-6CSF1Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YAL047C4.701.33E-6SPC72Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization
YNL231C4.661.58E-6PDR16Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress
YBR159W4.582.38E-6IFA38Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides
YML062C4.454.22E-6MFT1Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance
YNR006W4.395.63E-6VPS27Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)

GO enrichment analysis for SGTC_376
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2622.55E-93SGTC_3773970-0964 33.1 μMChemDiv (Drug-like library)164812350.303797
0.2055.85E-57SGTC_22006600604 21.9 μMChembridge (Fragment library)29047770.25tubulin folding & SWR complex
0.1925.05E-50SGTC_1503970-0969 77.2 μMChemDiv (Drug-like library)66156400.397436
0.1653.69E-37SGTC_2103551-0445 56.8 μMChemDiv (Drug-like library)28651800.136364
0.1591.30E-34SGTC_20775212015 200.0 μMChembridge (Fragment library)7809000.0519481
0.1553.01E-33SGTC_21415621638 7.7 μMChembridge (Fragment library)28562380.378788tubulin folding & SWR complex
0.1482.97E-30SGTC_5891227-0070 34.1 μMChemDiv (Drug-like library)28413840.178082
0.1462.38E-29SGTC_1683970-0862 530.5 μMChemDiv (Drug-like library)31351861
0.1453.95E-29SGTC_8470342-0274 5.5 μMChemDiv (Drug-like library)180970.262295
0.1454.50E-29SGTC_286k060-0067 22.9 μMChemDiv (Drug-like library)59850510.0666667tubulin folding & SWR complex
0.1426.49E-28SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.0779221TSC3-RPN4
0.1419.00E-28SGTC_229benomyl 22.9 μMMiscellaneous287800.0674157tubulin folding & SWR complex
0.1403.19E-27SGTC_23196033012 152.6 μMChembridge (Fragment library)8472460.05tubulin folding & SWR complex
0.1399.34E-27SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.0641026
0.1368.42E-26SGTC_21615662504 8.5 μMChembridge (Fragment library)28593340.246154tubulin folding & SWR complex

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1683970-0862530.54 μM13135186ChemDiv (Drug-like library)330.292160.54116
SGTC_1503970-096977.2 μM0.3974366615640ChemDiv (Drug-like library)450.78341.13809
SGTC_214156216387.67 μM0.3787882856238Chembridge (Fragment library)289.713681.71303tubulin folding & SWR complex
SGTC_3803970-09686 μM0.3717952891601ChemDiv (Drug-like library)416.338340.47309
SGTC_20985376439882.5 nM0.3593752844116Chembridge (Fragment library)255.268621.04903
SGTC_1493970-082246.1 μM0.3333333114250ChemDiv (Drug-like library)313.347761.90504tubulin folding & SWR complex
SGTC_1893970-07422.12 μM0.3064522891807ChemDiv (Drug-like library)297.06172.03104
SGTC_3773970-096433.1 μM0.30379716481235ChemDiv (Drug-like library)413.377460.31908
SGTC_3793970-09725.9 μM0.32898042ChemDiv (Drug-like library)401.366760.56208tubulin folding & SWR complex
SGTC_2144373-349670 μM0.2976193779166ChemDiv (Drug-like library)443.403440.78309